CN104126497A - Method for breeding high-stress-resistance giant embryo rice sterile line - Google Patents
Method for breeding high-stress-resistance giant embryo rice sterile line Download PDFInfo
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- CN104126497A CN104126497A CN201410332924.1A CN201410332924A CN104126497A CN 104126497 A CN104126497 A CN 104126497A CN 201410332924 A CN201410332924 A CN 201410332924A CN 104126497 A CN104126497 A CN 104126497A
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- giant embryo
- alkali tolerance
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 117
- 235000009566 rice Nutrition 0.000 title claims abstract description 117
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 97
- 238000000034 method Methods 0.000 title claims abstract description 8
- 238000009395 breeding Methods 0.000 title abstract description 8
- 230000001488 breeding effect Effects 0.000 title abstract description 8
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 116
- 230000000306 recurrent effect Effects 0.000 claims abstract description 25
- 239000003513 alkali Substances 0.000 claims description 68
- 150000003839 salts Chemical class 0.000 claims description 48
- 201000010099 disease Diseases 0.000 claims description 29
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 29
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 28
- 239000011780 sodium chloride Substances 0.000 claims description 28
- 241000196324 Embryophyta Species 0.000 claims description 25
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 25
- 238000002474 experimental method Methods 0.000 claims description 20
- 238000009396 hybridization Methods 0.000 claims description 19
- 235000021329 brown rice Nutrition 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 238000009331 sowing Methods 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 230000000379 polymerizing effect Effects 0.000 abstract 2
- 230000009418 agronomic effect Effects 0.000 abstract 1
- 238000012423 maintenance Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 230000007850 degeneration Effects 0.000 description 22
- 239000002689 soil Substances 0.000 description 11
- 230000002595 cold damage Effects 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 4
- 238000013475 authorization Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 229960003692 gamma aminobutyric acid Drugs 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/13—Abiotic stress
- Y02A40/135—Plants tolerant to salinity
Abstract
The invention discloses a method for breeding a high-stress-resistance giant embryo rice sterile line, and belongs to the technical field of stress-resistant breeding of special rice. The method comprises the following steps: polymerizing three types of stress-resistant rice resistant resource genes, namely, a drought-resistant variety Lvhan No.1, a hardy variety Dongye No.1 and a saline-alkaline-tolerant variety Dongdao No.4 serving as resistant resource parents into a giant embryo rice three-line maintenance line geB serving as recurrent parent into giant embryo rice three-line maintainer line geB serving as a recurrent parent through a hybridizing, backcrossing and polymerizing method; performing backcrossing and screening for a plurality of generations to breed a giant embryo three-line maintainer line (stress-resistant geB system) which has agronomic and yield characters being kept highly consistent with the original giant embryo rice three-line maintainer system as well as high stress resistance; and hybridizing and backcrossing the stress-resistant geB system and a corresponding giant embryo rice sterile line for three generations to breed a high-stress-resistance giant embryo rice sterile line, and breeding giant embryo hybrid rice with high nutrition and a high stress resistance level by taking the high-stress-resistance giant embryo rice sterile line as a female parent.
Description
Technical field
The present invention is specifically related to a kind of selection of high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line, belongs to Special Rices resistance breeding technical field.
Background technology
Characters of Giant Embryo Rice is a kind of paddy rice with huge embryo, greatly improves because the increase of embryo makes the nutritive value of rice, has unique and potential economic use value.Giant embryo rice includes natural nutrition element and the physiological activators such as abundant γ-aminobutyric acid, protein and several mineral materials, amino acid, vitamin, and normal food can improve health, and regulates body ' s physiological rhythm.Paddy rice is called paddy rice anti contravariance to various stress factors as the resist ability of cold-resistant, drought resisting, anti-salt etc.In recent years, Global Natural Disasters frequency is many, population increase, shortage of water resources, soil alkaline desertification and agricultural workforce's shortage etc. simultaneously increasingly sharpen, that brings thus variously coerces the production that directly has influence on paddy rice, saline and alkaline, arid, low temperature etc. become the main cause that affects rice yield, in recent years breeding man resistance breeding mechanism and resistance breeding have been made to large quantity research, and have made some progress, also be bred as some resistance kinds, but be mostly single resistance kind.Cultivate comprehensive Salt And Alkali Tolerance, the high yielding variety that drought-resistant, lower temperature resistance is strong for breaking through the predicament in short supply of current China's resistance kind, the severe times of ecotope such as reply arid, saline land, for Food Security in China increases new technological reserve, ensure that national food has important practical significance safely.The inventive method is in maintainer material by the anti-source gene pyramiding of multiple paddy rice anti contravariance to Characters of Giant Embryo Rice three by the hybridization polymerization that backcrosses, being bred as agronomy is that maintainer height is consistent with yield traits with former Characters of Giant Embryo Rice three, the Characters of Giant Embryo Rice three with high resistance to cold and diseases is maintainer, backcross with corresponding Characters of Giant Embryo Rice male sterile line hybridization by resistance Characters of Giant Embryo Rice maintainer again, be bred as high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line, preparing three with its is that giant embryo hybrid rice can improve resistance level, adapt to arid, cold damage, the harsh weather such as saline land and soil condition, reduce the impact of disaster, ensure output.
summary of the invention
The object of the present invention is to provide a kind of selection of high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line, thereby greatly improve the resistance level of Characters of Giant Embryo Rice male sterile line.This method is with green non-irrigated No. 1 (authorization numbering: state examines rice 2005053 of drought-resistant variety, country's variety of crops power protection, notification number: CNA002893E), wild No. one of cold-resistant variety east (Chen great Zhou. Dongxiang Wild Rice cold resistance research and apply ［ D ］. Nanjing: Agricultural University Of Nanjing, 2004), Salt And Alkali Tolerance kind east rice No. four (authorization numberings: lucky careful rice 2010005) is resistant parent, be maintainer 1813geB with Characters of Giant Embryo Rice three, TgeB and the seed selection of Crop Genetic Breeding research institute of 98-14geB(University Of Agriculture and Forestry In Fujian) be recurrent parent, be in maintainer by the anti-source gene pyramiding of these 3 kinds of paddy rice anti contravariances to Characters of Giant Embryo Rice three by the hybridization polymerization that backcrosses, backcross and screen through many generations, be bred as giant embryo proterties, nutrient composition content, agronomy is consistent with former Characters of Giant Embryo Rice maintainer height with yield traits, the Characters of Giant Embryo Rice three with high resistance to cold and diseases is maintainer, degeneration-resistant Characters of Giant Embryo Rice three is that maintainer is hybridized and 3 generations that backcrossed with corresponding Characters of Giant Embryo Rice male sterile line, can be bred as high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line, preparing three with its is that giant embryo hybrid rice can improve resistance level, adapt to arid, cold damage, the harsh weather such as saline land and soil condition, ensure the yield level of giant embryo hybrid rice.
For reaching above object, the present invention by the following technical solutions:
A selection for high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line, comprises the following steps:
1) selecting Salt And Alkali Tolerance kind east rice No. four (A), cold-resistant variety east wild No. one (B), drought-resistant varieties green non-irrigated No. 1 (C) is resistant parent, and Characters of Giant Embryo Rice three is that maintainer geB is recurrent parent (D), makes hybridization A/B, must hybridize F
1in generation,, with F
1-ABrepresent;
2) plantation F
1-ABwith green non-irrigated No. 1 (C), make hybridization C/F in flowering stage
1-AB, F must reestablish diplomatic relations
1(F
1-ABC), plantation F
1-ABC, selfing obtains F
2-ABC;
3) plantation F
2-ABCwith recurrent parent geB(D), F
2-ABCcolony is in 1000-1200 strain, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select the individual plant of listing mark and agronomy and yield traits excellence flowering stage, make hybridization D/F
2-ABCobtain F
1-ABCD;
4) plantation F
1-ABCD, selfing obtains F
2-ABCD, by F
2-ABCDseed shells into brown rice, select the brown rice sowing with giant embryo proterties, colony is in 500-600 strain, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, D/F backcrosses
2-ABCDobtain first backcross generation BC
1f
1;
5) plantation BC
1f
1, selfing obtains BC
1f
2, plantation BC
1f
2colony is in 500-600 strain, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select the individual plant of listing mark and agronomy and yield traits excellence flowering stage, D/ BC backcrosses
1f
2obtain second backcross generation BC
2f
1;
6) continue to be returned to BC by the program of step 5)
4f
1, selfing obtains BC
4f
2generation, plantation BC
4f
2generation, plant the giant embryo male sterile line geA that recurrent parent geB is corresponding simultaneously, seedling stage is to BC
4f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, the high drought resisting of listing mark, height resist cold, the rice seedling of high Salt And Alkali Tolerance, and selecting flowering stage economical character excellence, the individual plant the most similar to recurrent parent geB can be fixed to high resistance to cold and diseases giant embryo three is maintainer (degeneration-resistant geB is);
7) degeneration-resistant geB system and former geB are that corresponding giant embryo male sterile line geA is hybridized and 3 generations that backcrossed, and can be bred as high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line (degeneration-resistant geA is).
The selection of high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line recited above, its recurrent parent giant embryo three is that maintainer geB is 1813geB, TgeB or 98-14geB, its corresponding Characters of Giant Embryo Rice male sterile line geA is 1813geA, TgeA or 98-14geA.
The selection of high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line recited above, the high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line of its incubation has the characteristic of giant embryo proterties, drought-resistant, cold-resistant, Salt And Alkali Tolerance simultaneously.
beneficial effect of the present invention:
The present invention is respectively controlling drought resisting, cold-resistant, the anti-source gene of Salt And Alkali Tolerance imports in the male sterile line of Special Rices Characters of Giant Embryo Rice by hybridization and back cross breeding method, be bred as identical with former Characters of Giant Embryo Rice male sterile line genetic background but there is the Characters of Giant Embryo Rice male sterile line of high resistance to cold and diseases, the Characters of Giant Embryo Rice male sterile line agronomy of high resistance to cold and diseases is consistent with former Characters of Giant Embryo Rice male sterile line height with yield traits, still possesses giant embryo, high nutrition, high yield, high-quality characteristic, but the giant embryo hybrid rice good stress resistance of the Characters of Giant Embryo Rice male sterile line of high resistance to cold and diseases preparation, can adapt to arid, cold damage, the harsh weather such as saline land and soil condition, under harsh weather and soil condition, every mu can increase output 20-26% than check variety.
Embodiment
The present invention further illustrates the present invention with the following example, but protection scope of the present invention is not limited to the following example.
Embodiment 1
1) selecting Salt And Alkali Tolerance kind east rice No. four (A), cold-resistant variety east wild No. one (B), drought-resistant varieties green non-irrigated No. 1 (C) is resistant parent, and Characters of Giant Embryo Rice three is that maintainer 1813geB is recurrent parent (D), makes hybridization A/B, must hybridize F
1in generation,, with F
1-ABrepresent;
2) plantation F
1-ABwith green non-irrigated No. 1 (C), make hybridization C/F in flowering stage
1-AB, F must reestablish diplomatic relations
1(F
1-ABC), plantation F
1-ABC, selfing obtains F
2-ABC;
3) plantation F
2-ABCwith recurrent parent 1813geB(D), F
2-ABCcolony is in 1000 strains, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, make hybridization D/F
2-ABCobtain F
1-ABCD;
4) plantation F
1-ABCD, selfing obtains F
2-ABCD, by F
2-ABCDseed shells into brown rice, select the brown rice sowing with giant embryo proterties, colony is in 500 strains, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, D/F backcrosses
2-ABCDobtain first backcross generation BC
1f
1;
5) plantation BC
1f
1, selfing obtains BC
1f
2, plantation BC
1f
2colony is in 500 strains, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, D/ BC backcrosses
1f
2obtain second backcross generation BC
2f
1;
6) continue to be returned to BC by the program of step 5)
4f
1, selfing obtains BC
4f
2generation, plantation BC
4f
2generation, plant the giant embryo male sterile line 1813geA that recurrent parent 1813geB is corresponding simultaneously, seedling stage is to BC
4f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, the high drought resisting of listing mark, height resist cold, the rice seedling of high Salt And Alkali Tolerance, and selecting flowering stage economical character excellence, individual plant 5 strains the most similar to recurrent parent 1813geB can be fixed to high resistance to cold and diseases giant embryo three is maintainer " degeneration-resistant 1813geB ";
7) degeneration-resistant 1813geB and 1813geA are hybridized and 3 generations that backcrossed, and can be bred as high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line " degeneration-resistant 1813geA ".
" degeneration-resistant 1813geA " has the characteristic of giant embryo proterties, drought-resistant, cold-resistant, Salt And Alkali Tolerance simultaneously." degeneration-resistant 1813geA " not only has giant embryo proterties with the giant embryo hybrid rice " degeneration-resistant 1813geA/geR186 " of giant embryo restorer geR186 preparation, nutrient composition content is high, also there is good stress resistance, can adapt to harsh weather and the soil conditions such as arid, cold damage, saline land, under harsh weather and soil condition, every mu increases output 20% than check variety " 1813geA/geR186 ".
Embodiment 2:
1) selecting Salt And Alkali Tolerance kind east rice No. four (A), cold-resistant variety east wild No. one (B), drought-resistant varieties green non-irrigated No. 1 (C) is resistant parent, and Characters of Giant Embryo Rice three is that maintainer TgeB is recurrent parent (D), makes hybridization A/B, must hybridize F
1in generation,, with F
1-ABrepresent;
2) plantation F
1-ABwith green non-irrigated No. 1 (C), make hybridization C/F in flowering stage
1-AB, F must reestablish diplomatic relations
1(F
1-ABC), plantation F
1-ABC, selfing obtains F
2-ABC;
3) plantation F
2-ABCwith recurrent parent TgeB(D), F
2-ABCcolony is in 1200 strains, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, make hybridization D/F
2-ABCobtain F
1-ABCD;
4) plantation F
1-ABCD, selfing obtains F
2-ABCD, by F
2-ABCDseed shells into brown rice, select the brown rice sowing with giant embryo proterties, colony is in 600 strains, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, D/F backcrosses
2-ABCDobtain first backcross generation BC
1f
1;
5) plantation BC
1f
1, selfing obtains BC
1f
2, plantation BC
1f
2colony is in 600 strains, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, D/ BC backcrosses
1f
2obtain second backcross generation BC
2f
1;
6) continue to be returned to BC by the program of step 5)
4f
1, selfing obtains BC
4f
2generation, plantation BC
4f
2generation, plant the giant embryo male sterile line TgeA that recurrent parent TgeB is corresponding simultaneously, seedling stage is to BC
4f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, the high drought resisting of listing mark, height resist cold, the rice seedling of high Salt And Alkali Tolerance, and selecting flowering stage economical character excellence, individual plant 5 strains the most similar to recurrent parent TgeB can be fixed to high resistance to cold and diseases giant embryo three is maintainer " degeneration-resistant TgeB ";
7) degeneration-resistant TgeB and TgeA are hybridized and 3 generations that backcrossed, and can be bred as high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line " degeneration-resistant TgeA ".
" degeneration-resistant TgeA " has the characteristic of giant embryo proterties, drought-resistant, cold-resistant, Salt And Alkali Tolerance simultaneously." degeneration-resistant TgeA " not only has giant embryo proterties with the giant embryo hybrid rice " degeneration-resistant TgeA/geR186 " of giant embryo restorer geR186 preparation, nutrient composition content is high, also there is good stress resistance, can adapt to harsh weather and the soil conditions such as arid, cold damage, saline land, under harsh weather and soil condition, every mu increases output 26% than check variety " TgeA/geR186 ".
Embodiment 3:
1) selecting Salt And Alkali Tolerance kind east rice No. four (A), cold-resistant variety east wild No. one (B), drought-resistant varieties green non-irrigated No. 1 (C) is resistant parent, and Characters of Giant Embryo Rice three is that maintainer 98-14geB is recurrent parent (D), makes hybridization A/B, must hybridize F
1in generation,, with F
1-ABrepresent;
2) plantation F
1-ABwith green non-irrigated No. 1 (C), make hybridization C/F in flowering stage
1-AB, F must reestablish diplomatic relations
1(F
1-ABC), plantation F
1-ABC, selfing obtains F
2-ABC;
3) plantation F
2-ABCwith recurrent parent 98-14geB(D), F
2-ABCcolony is in 1100 strains, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, make hybridization D/F
2-ABCobtain F
1-ABCD;
4) plantation F
1-ABCD, selfing obtains F
2-ABCD, by F
2-ABCDseed shells into brown rice, select the brown rice sowing with giant embryo proterties, colony is in 550 strains, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, D/F backcrosses
2-ABCDobtain first backcross generation BC
1f
1;
5) plantation BC
1f
1, selfing obtains BC
1f
2, plantation BC
1f
2colony is in 550 strains, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select individual plant 5 strains of listing mark and agronomy and yield traits excellence flowering stage, D/ BC backcrosses
1f
2obtain second backcross generation BC
2f
1;
6) continue to be returned to BC by the program of step 5)
4f
1, selfing obtains BC
4f
2generation, plantation BC
4f
2generation, plant the giant embryo male sterile line 98-14geA that recurrent parent 98-14geB is corresponding simultaneously, seedling stage is to BC
4f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, the high drought resisting of listing mark, height resist cold, the rice seedling of high Salt And Alkali Tolerance, and selecting flowering stage economical character excellence, individual plant 5 strains the most similar to recurrent parent 98-14geB can be fixed to high resistance to cold and diseases giant embryo three is maintainer " degeneration-resistant 98-14geB ";
7) degeneration-resistant 98-14geB and 98-14geA are hybridized and 3 generations that backcrossed, and can be bred as high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line " degeneration-resistant 98-14geA ".
" degeneration-resistant 98-14ge " has the characteristic of giant embryo proterties, drought-resistant, cold-resistant, Salt And Alkali Tolerance simultaneously." degeneration-resistant 98-14geA " not only has giant embryo proterties with the giant embryo hybrid rice " degeneration-resistant 98-14geA/geR186 " of giant embryo restorer geR186 preparation, nutrient composition content is high, also there is good stress resistance, can adapt to harsh weather and the soil conditions such as arid, cold damage, saline land, under harsh weather and soil condition, every mu increases output 23% than check variety " 98-14geA/geR186 ".
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (3)
1. a selection for high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line, is characterized in that: described selection comprises the following steps:
1) selecting Salt And Alkali Tolerance kind east rice No. four (A), cold-resistant variety east wild No. one (B), drought-resistant varieties green non-irrigated No. 1 (C) is resistant parent, and Characters of Giant Embryo Rice three is that maintainer geB is recurrent parent (D), makes hybridization A/B, must hybridize F
1in generation,, with F
1-ABrepresent;
2) plantation F
1-ABwith green non-irrigated No. 1 (C), make hybridization C/F in flowering stage
1-AB, F must reestablish diplomatic relations
1(F
1-ABC), plantation F
1-ABC, selfing obtains F
2-ABC;
3) plantation F
2-ABCwith recurrent parent geB(D), F
2-ABCcolony is in 1000-1200 strain, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select the individual plant of listing mark and agronomy and yield traits excellence flowering stage, make hybridization D/F
2-ABCobtain F
1-ABCD;
4) plantation F
1-ABCD, selfing obtains F
2-ABCD, by F
2-ABCDseed shells into brown rice, select the brown rice sowing with giant embryo proterties, colony is in 500-600 strain, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select the individual plant of listing mark and agronomy and yield traits excellence flowering stage, D/F backcrosses
2-ABCDobtain first backcross generation BC
1f
1;
5) plantation BC
1f
1, selfing obtains BC
1f
2, plantation BC
1f
2colony is in 500-600 strain, carry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, high drought resisting, high rice shoot cold-resistant, high Salt And Alkali Tolerance are carried out to listing mark, select the individual plant of listing mark and agronomy and yield traits excellence flowering stage, D/ BC backcrosses
1f
2obtain second backcross generation BC
2f
1;
6) continue to be returned to BC by the program of step 5)
4f
1, selfing obtains BC
4f
2generation, plantation BC
4f
2generation, plant the giant embryo male sterile line geA that recurrent parent geB is corresponding simultaneously, seedling stage is to BC
4f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, and the rice seedling of the high drought resisting of listing mark, high cold-resistant, high Salt And Alkali Tolerance is selected economical character excellence, the individual plant the most similar to recurrent parent geB flowering stage, and being fixed to high resistance to cold and diseases giant embryo three is maintainer;
7) high resistance to cold and diseases giant embryo three be maintainer is that corresponding giant embryo male sterile line geA is hybridized and 3 generations that backcrossed with former geB, is bred as high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line.
2. the selection of a kind of high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line according to claim 1, it is characterized in that: recurrent parent Characters of Giant Embryo Rice three is that maintainer geB is 1813geB, TgeB or 98-14geB, its corresponding Characters of Giant Embryo Rice male sterile line geA is 1813geA, TgeA or 98-14geA.
3. a high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line for the method for claim 1 seed selection, is characterized in that: the high resistance to cold and diseases Characters of Giant Embryo Rice male sterile line of incubation has the characteristic of giant embryo proterties, high nutrient composition content, drought-resistant, cold-resistant, Salt And Alkali Tolerance.
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Cited By (4)
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| CN104488700A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of CMS lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
| CN104488698A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Streak leaf long-stigma elongated-spike-neck light body three-line sterile line breeding method |
| CN104585019A (en) * | 2015-01-15 | 2015-05-06 | 福建农林大学 | Breeding method of erect panicle type long stigma and elongated upmost internode three-line sterile line |
| CN110249942A (en) * | 2019-07-29 | 2019-09-20 | 海南广陵高科实业有限公司 | A kind of hybrid rice breeding method |
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| CN1429479A (en) * | 2001-12-30 | 2003-07-16 | 福建农林大学 | Breeding method of large embryo rice variety |
| CN103125368A (en) * | 2013-03-13 | 2013-06-05 | 福建农林大学 | Breeding method of insect-resistant big-stigma three-line sterile line |
| CN103891603A (en) * | 2014-04-04 | 2014-07-02 | 福建农林大学 | Breeding method for multifunctional rice three-line sterile line |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1429479A (en) * | 2001-12-30 | 2003-07-16 | 福建农林大学 | Breeding method of large embryo rice variety |
| CN103125368A (en) * | 2013-03-13 | 2013-06-05 | 福建农林大学 | Breeding method of insect-resistant big-stigma three-line sterile line |
| CN103891603A (en) * | 2014-04-04 | 2014-07-02 | 福建农林大学 | Breeding method for multifunctional rice three-line sterile line |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488700A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of CMS lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
| CN104488698A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Streak leaf long-stigma elongated-spike-neck light body three-line sterile line breeding method |
| CN104585019A (en) * | 2015-01-15 | 2015-05-06 | 福建农林大学 | Breeding method of erect panicle type long stigma and elongated upmost internode three-line sterile line |
| CN104488700B (en) * | 2015-01-15 | 2016-01-06 | 福建农林大学 | The selection of the glutinous CMS line of purple leaf upright spike length fringe neck |
| CN104585019B (en) * | 2015-01-15 | 2016-06-08 | 福建农林大学 | The selection of the long fringe neck CMS line of erect head long stigma |
| CN110249942A (en) * | 2019-07-29 | 2019-09-20 | 海南广陵高科实业有限公司 | A kind of hybrid rice breeding method |
| CN110249942B (en) * | 2019-07-29 | 2021-07-20 | 海南广陵高科实业有限公司 | Hybrid rice breeding method |
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