CN110073972A - A kind of selection of purple leaf white flower cabbage type rape hybrid combination - Google Patents
A kind of selection of purple leaf white flower cabbage type rape hybrid combination Download PDFInfo
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- CN110073972A CN110073972A CN201910486088.5A CN201910486088A CN110073972A CN 110073972 A CN110073972 A CN 110073972A CN 201910486088 A CN201910486088 A CN 201910486088A CN 110073972 A CN110073972 A CN 110073972A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of selections of purple leaf white flower cabbage type rape hybrid combination.Selection of the invention, include the following steps: that (one) purple leaf white flower keeps system and sterile line breeding: being to keep being in white flower Shanghai oil 17 by the greenery white flower cabbage type rape cytoplasmic male sterility that purple green vegetables purple leaf character transformation to deposit number is CGMCC No.17384, breeding purple leaf white flower cabbage type rape cytoplasmic male sterility keeps system.(2) purple leaf white flower breeding of hybrid combination: purple leaf white flower sterile line hybridizes with common greenery cabbage type rape cytoplasmic male sterility restorer, the combination of breeding purple leaf white flower cross-bred rape.It can the combination of breeding purple leaf white flower Wild cabbage type cytoplasmic male sterility cross-bred rape using this method.
Description
Technical field
The invention belongs to rapeseed breeding fields, are related to a kind of selection of purple leaf white flower cabbage type rape hybrid combination.
Background technique
Rape is one of big oil crops in China three, and the rapeseed area and yield in China account for about the world 30%.In recent years,
Due to Rape-seed production Mechanization Level and comparative effectiveness is relatively low that China's rapeseed cultivation area is caused to decline year after year.Rape oil removing
With outside basic function, also having the function of that dish is used, fertilizer is used, feeding, sweet use and ornamental etc..It gives full play to the multifunctionality of rape, add
Fast rape comprehensive exploitation and utilization are to improve Rape-seed production benefit, stablize rapeseed cultivation area, push the restorative increasing of Rape-seed production
Long effective way.
Cabbage type rape blade is usually green, and flower is usually white, and cabbage type rape purple leaf character and white flower character are all
It is a kind of incomplete dominance character.Blade purple Producing reason is accumulation of the anthocyanin in blade.Anthocyanin is as a kind of
Plant time metabolite, is widely present in the cytoplasm of the organs such as root, stem, leaf, flower, the fruit of plant.Purple leaf Wild cabbage type oil
The anthocyanin that dish is rich in has many biological functions such as anti-oxidant, antitumor, is a kind of guarantor with fine potentiality to be exploited
Strong vegetables；In addition, purple leaf white flower rape can be used as in seedling stage sees leaf crop, the florescence is used for landscape moulding as flower crop is seen.
Summary of the invention
An object of the present invention is to provide a kind of selection of purple leaf white flower cabbage type rape holding system.
A kind of purple leaf white flower cabbage type rape cytoplasmic male sterility provided by the invention keeps the selection of system, be by
The greenery white flower cabbage type rape cytoplasmic male sterility that purple green vegetables purple leaf character transformation is CGMCC No.17384 to deposit number
Holding is in white flower Shanghai oil 17, and breeding purple leaf white flower cabbage type rape cytoplasmic male sterility keeps system.
In the above method, the purple leaf character transformation by purple green vegetables to deposit number is that the greenery of CGMCC No.17384 are white
Flower cabbage type rape cytoplasmic male sterility keeps the method in system to include the following steps:
A) using the purple green vegetables as male parent, keep system for mother with the greenery white flower cabbage type rape cytoplasmic male sterility
This, is hybridized, and hybrid generation F is obtained1；
B) by the hybrid generation F1Selfing obtains F2Plant；
C) F is chosen2Seedling stage leaf is most purple in plant, florescence most white and 100% test cross infertility the single plant of flower is non-circulation
Parent keeps system for recurrent parent with the greenery white flower cabbage type rape cytoplasmic male sterility, carries out 3 backcrossings, obtains
Backcross progeny BC3F1Plant；
D) from the backcross progeny BC3F1Selection seedling stage leaf is most purple, florescence colored most white and 100% test cross infertility in plant
Individual plant selfing obtains BC3F2For plant, BC is chosen3F2, florescence flower most white and yield highest most purple for selection seedling stage leaf in plant
Strain, obtain purple leaf white flower cabbage type rape cytoplasmic male sterility and keep system.
In the above method, 3 backcrossings are respectively that 100% test cross infertility and seedling stage leaf are chosen from last time backcross progeny
The most white plant of most purple and florescence flower is selfed, then choose from self progeny 100% test cross infertility and seedling stage leaf it is most purple and
The most white plant of florescence flower is as the nonrecurrent parent being returned next time；
Above-mentioned 100% test cross infertility refers to the strain and maternal cabbage type rape cytoplasmic male sterile line (such as Qin-you No.7
(state examines oil 2004014) selfing separation sterile plant) test cross, make 100% test cross infertility character of sterile line.
And/or 3 backcrossings include as follows
C-1, the F is chosen2Seedling stage leaf is most purple in plant and florescence most white and 100% test cross infertility the single plant of flower is non-wheel
Parent is returned, keeps system for recurrent parent with the greenery white flower cabbage type rape cytoplasmic male sterility, is returned 1 time, is returned
Hand over offspring BC1F1Plant；Again from the backcross progeny BC1F1Selection seedling stage leaf is most purple in plant and florescence flower is most white and 100% surveys
The individual plant selfing for handing over infertility, obtains BC1F2Plant；
C-2, the BC is chosen1F2The single plant that 100% test cross is sterile in plant and seedling stage leaf is most purple and florescence flower is most white is non-
Recurrent parent keeps system for recurrent parent, is returned 2 times, obtains with the greenery white flower cabbage type rape cytoplasmic male sterility
Backcross progeny BC2F1Plant；Again from the backcross progeny BC2F1Choose that seedling stage leaf is most purple and florescence flower is most white in plant and 100%
The individual plant selfing of test cross infertility, obtains BC2F2Plant；
C-3, the BC is chosen2F2The single plant that 100% test cross is sterile in plant and seedling stage leaf is most purple and florescence flower is most white is non-
Recurrent parent keeps system for recurrent parent, is returned 3 times, obtains with the greenery white flower cabbage type rape cytoplasmic male sterility
Backcross progeny BC3F1Plant.
In the above method, 100% test cross infertility is chosen into c-3 by the c-1 and seedling stage leaf is most purple and florescence flower is most white
Strain quantity is 20；
Or, choosing BC in the d3F2For 100% test cross infertility in plant and seedling stage leaf is most purple and florescence flower is most white and produces
Measuring highest strain quantity is 10；
Or, hybridizing in the method, being selfed, backcrossing and the place being selfed staggeredly carry out.
The cabbage type rape cytoplasmic male sterility strain is specially Qin-you No.7 male sterile plant.
Deposit number is that the holding of CGMCC NO.17384 greenery white flower cabbage type rape cytoplasmic male sterility is white flower Shanghai oil
17 application in the breeding that purple leaf white flower cabbage type rape cytoplasmic male sterility keeps system is also the scope of protection of the invention；
Or, the purple leaf white flower cabbage type rape cytoplasmic male sterility holding of above-mentioned breeding ties up to purple leaf white flower Wild cabbage type oil
Application in the breeding of dish cytoplasmic male sterile line is also the scope of protection of the invention；
Or, to tie up to purple leaf white flower Wild cabbage type thin for the purple leaf white flower cabbage type rape cytoplasmic male sterility holding of above-mentioned breeding
Application in the breeding of cytoplasmic male sterilty cross-bred rape combination is also the scope of protection of the invention.
Another object of the present invention is to provide a kind of breeding side of purple leaf white flower cabbage type rape cytoplasmic male sterile line
Method.
Method provided by the invention is that the purple leaf white flower cabbage type rape cytoplasmic male sterility of above-mentioned breeding is kept system
Wild cabbage type cytoplasmic male sterility cytoplasm is kept, purple leaf white flower cabbage type rape cytoplasmic male sterile line is obtained.
In the above method, described method includes following steps:
It 1) is female parent with the cabbage type rape cytoplasmic male sterility strain, with claim 1-4 either method breeding
It is male parent that purple leaf white flower cabbage type rape cytoplasmic male sterility, which keeps system, is hybridized, obtains hybrid generation F1；
2) from the sterile line hybrid generation F1The middle selection seedling stage leaf most most white and 100% test cross infertility of pale reddish brown phase flower is non-
Recurrent parent keeps system for recurrent parent, is returned, is obtained with the purple leaf white flower cabbage type rape cytoplasmic male sterility
Backcross progeny, choose that seedling stage leaf most pale reddish brown phase flower is most white and 100% test cross infertility (Anther degeneration, sterility thoroughly, without micro flower
Powder) strain be purple leaf white flower cytoplasmic male sterile line.
In the above method, the backcrossing number is 6 times；
And/or each backcrossing is that 100% test cross sterile (Anther degeneration, sterility are chosen from last time backcross progeny
Thoroughly, without trace-pollen) and seedling stage leaf is most purple and the florescence most white plant of flower is as the next nonrecurrent parent being returned.
In the above method, the cabbage type rape cytoplasmic male sterility strain is cabbage type rape Qin-you No.7 sterile plant.
3rd purpose of the invention is to provide a kind of choosing of purple leaf white flower Wild cabbage type cytoplasmic male sterility cross-bred rape combination
Educate method.
Method provided by the invention includes the following steps: the purple leaf white flower cabbage type rape for obtaining above method breeding
Cytoplasmic male sterile line hybridizes with common greenery cabbage type rape restorer, realizes purple leaf white flower cabbage type rape cytoplasm male
The sterility cross-bred combined breeding of property.
Strain entitled 7P71,7P76 or the 7P83 of the cabbage type rape restorer.
Above-mentioned 7P76,7P71 and 7P83 are respectively by Qinyou No. 8 (state examines oil 2004015), miscellaneous No. 8 of China's oil (careful oil of state
2004007), the complete fertile strain test cross of Zhongyouza No.8 (state examines oil 2004004) separation is 100% strain restored through system
Breeding forms.
Above-mentioned white flower Shanghai oil 17 for Shanghai oil 17 (breeding of crop institute, Shanghai academy of agricultural sciences, state examine oily 2011006) white flower mutant strain,
Deposit number be CGMCC No.17384, China Committee for Culture Collection of Microorganisms's common micro-organisms center, abbreviation CGMCC,
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101, preservation day be
On 2 28th, 2019, classification naming was cabbage type rape Brassica napus.
The experiment proves that purple leaf character transformation to white flower cabbage type rape cytoplasmic male sterility can be kept
In system, breeding purple leaf white flower cabbage type rape cytoplasmic male sterility keeps system；It is thin with the purple leaf white flower cabbage type rape of breeding
Cytoplasmic male sterilty keeps system to keep Wild cabbage type cytoplasmic male sterility cytoplasm, and breeding purple leaf white flower cabbage type rape is thin
Cytoplasm male sterility line；With purple leaf white flower cabbage type rape cytoplasmic male sterile line and common greenery cabbage type rape cytoplasm
The combination of male sterile restoring line preparing hybrid, and the strong advantage of high yield " purple Ye Baihua " hybridization group is screened from the cross combination of preparation
It closes.
Detailed description of the invention
Fig. 1 is that purple leaf white flower cabbage type rape cytoplasmic male sterility holding is breeding flow chart.
Fig. 2 is purple leaf white flower cabbage type rape cytoplasmic male sterile line breeding flow chart.
Fig. 3 is purple leaf white flower cabbage type rape cytoplasmic male sterility breeding of hybrid combination flow chart.
Fig. 4 is that purple leaf white flower holding is seedling stage.
Fig. 5 is purple leaf white flower sterile line seedling stage.
Fig. 6 is the purple leaf white flower cross combination production of hybrid seeds (centre is purple leaf sterile line, and both sides are greenery restorer).
Fig. 7 is that purple leaf white flower cross-bred rape combines (right side) and greenery rape seedling compares (left side).
Fig. 8 is purple leaf white flower cross combination seedling stage.
Fig. 9 is purple leaf white flower cross combination squaring period.
Figure 10 is purple leaf white flower cross combination single plant inflorescence (seedling stage purple leaf gradually takes off into green).
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1, breeding purple leaf white flower cabbage type rape cytoplasmic male sterility keep system
One, white flower cabbage type rape cytoplasmic male sterility holding is white flower Shanghai oil 17
White flower Shanghai oil 17, for Shanghai oil 17 (breeding of crop institute, Shanghai academy of agricultural sciences, state examine oil 2011006) white flower mutant strain,
Deposit number be CGMCC No.17384, China Committee for Culture Collection of Microorganisms's common micro-organisms center, abbreviation CGMCC,
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101, preservation day be
On 2 28th, 2019, classification naming was cabbage type rape Brassica napus.
Two, hybridize transformation
In March, 2010 carries out hybridization transformation according to Fig. 1:
1, hybridize
In March, 2010 is in Hefei with 2 plants of purple green vegetables (Fang Zhenzi green vegetables, production number: 7095, Hebei Qing County rich kind of industry of grain grain)
It is white flower Shanghai oil 17 for female parent with 5 plants of white flower cabbage type rape cytoplasmic male sterility holdings, hybridization obtains 10 parts for male parent
Filial generation F1Seed；
2, it is selfed
In May, 2010, by 10 parts of F of harvest1Hybrid seed takes Qinghai to, and in July, 2010 is from 10 parts of F1Middle selection seedling stage leaf
The most white 20 plants of bagging selfings of single plant of most purple and florescence flower, obtain 20 plants of F2Selfed seed；
3, repeatedly backcrossing
1) it is returned for the first time
In September, 2010, in 20 plants of F of plantation Qinghai, Hefei harvest2Selfed seed, every plant of F2It is selfed strain and plants 2 rows, totally 20
A F2Strain.In March, 2011 is from 20 F220 plants of Ye Zi, Hua Bai are selected in strain and 100% test cross infertility (refers to and female parent
(such as isolated sterile plant test cross of Qin-you No.7 (state examines oil 2004014) selfing, makes not cabbage type rape cytoplasmic male sterile line
Educating is 100% holding infertility character) it is used as male parent (nonrecurrent parent), 10 plants of white flower Shanghai oil 17 are maternal (recurrent parent), are returned
It hands over, obtains 20 parts of 1 generation group BC of backcrossing1F1Seed；
July in 2011 20 parts of 1 generation group BC of backcrossing1F1The corresponding BC of seed1F1Select seedling stage leaf most purple in strain and the florescence
The 20 plants of bagging selfings of most white and test cross 100% test cross infertility the single plant of flower, obtain 20 plants of selfing strain seeds and are denoted as BC1F2。
In September, 2011, in 20 plants of BC of plantation Qinghai, Hefei harvest1F2Seed obtains BC1F2Plant, every plant of 2 rows of plantation,
Totally 20 BC1F2Strain.
2) the 2nd backcrossing
In March, 2012 is from 20 BC1F2100% test cross infertility is selected in strain and seedling stage leaf is most purple and florescence flower is most white
20 plants of single plant；With this 20 plants of BC1F2As male parent (nonrecurrent parent), 10 plants of white flower Shanghai oil 17 are maternal (recurrent parent), are returned
It hands over, obtains 20 parts of 2 generation group BC of backcrossing2F1Seed；
In May, 2012, by the 20 parts of test cross offsprings and 20 parts of BC of harvest2F1Seed takes Qinghai plantation to, obtains BC2F1It plants
Strain, in July, 2012 is from 20 parts of BC2F1100% test cross infertility is selected in plant and seedling stage leaf is most purple and the florescence spends most white single plant 20
Strain bagging selfing, obtains 20 plants of selfing strain seeds, is denoted as BC2F2；
In September, 2012, in 20 plants of BC of plantation Qinghai, Hefei harvest2F2Seed obtains BC2F2Plant, every plant of 2 rows of plantation,
Totally 20 BC2F2Strain.
3) the 3rd backcrossing
In March, 2013 is from 20 BC2F2100% test cross infertility is selected in strain and seedling stage leaf is most purple and florescence flower is most white
20 plants of single plant；With this 20 plants of BC2F2As male parent (nonrecurrent parent), 20 plants of white flower Shanghai oil 17 are maternal (recurrent parent), are returned
It hands over, obtains 20 parts of 3 generation group BC of backcrossing3F1Seed；
In May, 2013, by the 20 parts of test cross offsprings and 20 parts of BC of harvest3F1Seed takes Qinghai plantation to, obtains BC3F1It plants
Strain, in July, 2013 is from 20 parts of BC3F1100% test cross infertility is selected in plant and seedling stage leaf is most purple and the florescence spends most white single plant 10
Strain bagging selfing, obtains 10 plants of selfing strains and is denoted as BC3F2Seed；
10 parts of BC of Qinghai harvest are planted in September, 2013, Hefei3F2Seed obtains BC3F2Plant, every part is planted 100 plants,
Totally 10 strains.
In March, 2014 is in 10 BC3F2Breeding is isolated with the nylon wire accounts of 100 mesh respectively before just spending in strain, at the florescence,
Each strain is each to take 5 plants and Qin-you No.7 (state examines oil 2004014) sterile plant test cross at random；The purple of 10 strains is investigated in seedling stage
Leaf character, florescence investigate pattern；10 strain test cross results are identified in Qinghai in July, 2014；After rape is mature, plant division system harvest is surveyed
It produces.5 plant of 100% test cross infertility of test cross is screened, sterility is thorough, and seedling stage leaf is most purple, the florescence spends the highest strain of most white and yield
System is kept as the purple leaf white flower cabbage type rape cytoplasmic male sterility of middle choosing, is named as purple white 202B.
It is seedling stage (Fig. 4) as can be seen that purple white 202B seedling stage from the holding of purple leaf white flower Brassica napus cytoplasmic male sterility
The purple visual character of leaf is obvious and consistent.
Embodiment 2, breeding purple leaf white flower Brassica napus cytoplasmic male sterile line
Keep the purple white 202B continuous backcross breeding of system purple based on Qin-you No.7 sterile plant and purple leaf white flower cytoplasmic male sterility
The purple white 202A of leaf white flower cytoplasmic male sterile line
From 2014, hybridization and backcross transformation are carried out according to Fig. 2:
1, hybridize
In March, 2014 is female parent with 10 plants of cabbage type rape Qin-you No.7s (state examines oil 2004014) sterile plant, with 10 plants of realities
It is male parent that the purple leaf white flower cabbage type rape cytoplasmic male sterility for applying 1 breeding of example, which keeps the purple white 202B of system, and hybridization obtains 10 parts
Strain hybrid generation F1；Male parent takes the selfing of powder strain bagging to reserve seed for planting.
2, it is returned
1) the 1st backcrossing
In July, 2014, from 10 parts of hybrid generation F1It is middle that the seedling stage leaf most pale reddish brown phase in offspring is selected to spend most white and 100% survey
Infertility is handed over (to refer to maternal cabbage type rape cytoplasmic male sterile line (as Qin-you No.7 (state examines oil 2004014) selfing separates
Sterile plant test cross makes sterile line 100% keep sterile character)) strain, it is denoted as F1-H17- A, totally 10 plants.
With 10 plants of F1-H17For-A as female parent, 10 plants of white 202B of purple are male parent, and backcrossing obtains 1 generation group BC of backcrossing1F1；Father
Originally the selfing of powder strain bagging is taken to reserve seed for planting.
2) the 2nd backcrossing
In March, 2015 is from 10 parts of 1 generation group BC of backcrossing1F1Middle selection seedling stage leaf most most white and 100% test cross of pale reddish brown phase flower is not
The single plant educated, is denoted as BC1F1-H17- A, totally 10 plants.
With 10 plants of BC1F1-H17- A is used as female parent, and the most pale reddish brown phase flower of seedling stage leaf is most white in 10 plants of white 202B of purple and 100% surveys
The single plant for handing over infertility is male parent, and backcrossing obtains 10 parts of 2 generation group BC of backcrossing2F1；Male parent takes the selfing of powder strain bagging to reserve seed for planting.
3) the 3rd backcrossing
In July, 2015 is from 10 parts of 2 generation group BC of backcrossing2F1Middle selection seedling stage leaf is most purple, the florescence spends most white and 100% test cross
The single plant of infertility (Anther degeneration, sterility thoroughly, without trace-pollen), is denoted as BC2F1-H17- A, totally 10 plants.
With 10 plants of BC2F1- A is male parent as female parent, 10 plants of white 202B of purple, and backcrossing obtains 10 parts of 3 generation groups of backcrossing
BC3F1；Male parent takes the selfing of powder strain bagging to reserve seed for planting.
4) the 4th is returned
In March, 2016 is from 10 plants of 3 generation group BC of backcrossing3F1Middle selection seedling stage leaf is most purple, the florescence spends most white and 100% test cross
The single plant of infertility (Anther degeneration, sterility thoroughly, without trace-pollen), is denoted as BC3F1-H17- A, totally 10 plants.
With 10 plants of BC3F1- A is male parent as female parent, 10 plants of white 202B of purple, and backcrossing obtains 10 parts of 4 generation groups of backcrossing
BC4F1；Male parent takes the selfing of powder strain bagging to reserve seed for planting.
5) the 5th is returned
In July, 2016 is from 10 parts of 4 generation group BC of backcrossing4F1Middle selection seedling stage leaf is most purple, the florescence spends most white and 100% test cross
The single plant of infertility (Anther degeneration, sterility thoroughly, without trace-pollen) is denoted as BC4F1-H17- A, totally 5 plants.
With 5 plants of BC4F1-H17- A is male parent as female parent, 5 plants of white 202B of purple, and backcrossing obtains 5 parts of 5 generation groups of backcrossing
BC5F1；Male parent takes the selfing of powder strain bagging to reserve seed for planting.
6) the 6th backcrossing
In March, 2017 is from 10 parts of 5 generation group BC of backcrossing5F1Middle selection seedling stage leaf is most purple, the florescence spends most white and 100% test cross
The single plant of infertility (Anther degeneration, sterility thoroughly, without trace-pollen) is denoted as BC5F1-H17- A, totally 5 plants.
With 5 plants of BC5F1-H17- A is male parent as female parent, 5 plants of white 202B of purple, and backcrossing obtains 5 parts of 6 generation groups of backcrossing
BC6F1；Male parent takes the selfing of powder strain bagging to reserve seed for planting.
3, sterile line is formed
In July, 2017, by 5 parts of 6 generation sterile line BC of backcrossing6F1-H17- 1, BC6F1-H17- 2, BC6F1-H17- 3, BC6F1-H17-
4, BC6F1-H17- 5 various 100,5 strain leaf colors of seedlings investigation, the florescence investigates pattern.Determine that seedling stage leaf is most purple, florescence flower
The BC of most white and 100% test cross infertility (Anther degeneration, sterility thoroughly, without trace-pollen)6F1-H17- 1 strain is the purple of middle choosing
Leaf white flower cytoplasmic male sterile line is named as purple white 202A (godfather originally takes powder strain to be determined as purple white 202B thereon).
From purple leaf white flower Brassica napus cytoplasmic male sterile line seedling stage (Fig. 5) as can be seen that purple white 202A purple Ye Xingzhuan
It is obvious and stable.
The purple white 202A petal of purple leaf white flower cytoplasmic male sterile line is about 0.5cm, wide 0.3cm, anther long 1.5mm, wide
0.6mm, the long 0.5cm of stamen, the long 1cm of gynoecium, the white triangle of anther, abortive degree are high.Solarium's production of hybrid seeds F1Sterile plant rate is low
In 0.1%.It blooms relatively smooth, nothing closes flower bud.Stable fertility and thoroughly.Plant height 155cm, branch amount 11, the time of infertility about 232 days,
It is 2 days more early than control (breeding of academy of agricultural sciences, the Anhui Province) breeding time of Anhui oil 14, single plant effective angle number about 400, about 22 of every seed number per pod
3.9 grams of mass of 1000 kernel.
Embodiment 3, purple leaf white flower breeding of hybrid combination
1, breeding
Screening is combined according to Fig. 3 within 2017:
In July, 2017 is female parent with the purple white 202A of the purple leaf white flower cytoplasmic male sterile line of the breeding of 50 plants of embodiments 2,
(7P76,7P71 and 7P83 are respectively by Qin You 8 by cabbage type rape restorer 7P76,7P71 and 7P83 of Agricultural University Of Anhui's breeding
Number (state examine oil 2004015), miscellaneous No. 8 of China's oil (state examines oil 2004007), Zhongyouza No.8 (state examines oil 2004004) the complete of separation can
Educating strain test cross is that 100% strain restored is formed through systematic breeding) it is paternal hybrid, obtain 3 parts of cross combination F1Seed.
In September, 2017 plants 3 parts of cross combinations each 100；In May, 2018 identifies cross combination F1。
Fig. 6 is the purple leaf white flower cross combination production of hybrid seeds (centre is purple leaf sterile line, and both sides are greenery restorer).Fig. 7 is purple
Leaf white flower cross-bred rape combines (right side) and greenery rape seedling comparison (left side).Fig. 8 is purple leaf white flower cross combination seedling stage.Fig. 9 is
Purple leaf white flower cross combination squaring period.Figure 10 is purple leaf white flower cross combination single plant inflorescence (seedling stage purple leaf gradually takes off into green).
2, purple leaf white flower cross-bred rape combination, which is surveyed, produces
Species test in May, 2018 simultaneously surveys production, and 3 combination species tests and survey produce and the results are shown in Table 1, it can be seen that it is high to match combination yield
And there is purple leaf white flower character.
Table 1 is with the economical character and yield result that purple leaf 202A is that 3 maternal purple leaves combine
Claims (10)
- It is by purple green vegetables purple Ye Xingzhuan 1. a kind of purple leaf white flower cabbage type rape cytoplasmic male sterility keeps the selection of system Transformation keeps being white flower Shanghai oil to the greenery white flower cabbage type rape cytoplasmic male sterility that deposit number is CGMCC No.17384 In 17, breeding purple leaf white flower cabbage type rape cytoplasmic male sterility keeps system.
- 2. according to the method described in claim 1, it is characterized by:The purple leaf character transformation by purple green vegetables is thin to the greenery white flower cabbage type rape that deposit number is CGMCC No.17384 Cytoplasmic male sterilty keeps the method in system to include the following steps:A) using the purple green vegetables as male parent, keep system for female parent with the greenery white flower cabbage type rape cytoplasmic male sterility, Hybridized, obtains hybrid generation F1；B) by the hybrid generation F1Selfing obtains F2Plant；C) F is chosen2Seedling stage leaf is most purple in plant, florescence most white and 100% test cross infertility the single plant of flower is nonrecurrent parent, It keeps system for recurrent parent with the greenery white flower cabbage type rape cytoplasmic male sterility, 3 backcrossings is carried out, after obtaining backcrossing For BC3F1Plant；D) from the backcross progeny BC3F1Choose that seedling stage leaf is most purple, florescence most white and 100% test cross infertility the single plant of flower in plant Selfing, obtains BC3F2For plant, BC is chosen3F2, florescence flower most white and yield highest strain most purple for selection seedling stage leaf in plant System obtains purple leaf white flower cabbage type rape cytoplasmic male sterility and keeps system.
- 3. method according to claim 1 or 2, it is characterised in that:100% test cross infertility is respectively chosen from last time backcross progeny for 3 backcrossings and seedling stage leaf is most purple and the florescence is spent most White plant is selfed, then the plant that 100% test cross is sterile and seedling stage leaf is most purple and florescence flower is most white is chosen from self progeny Strain is as the nonrecurrent parent being returned next time；And/or 3 backcrossings include as followsC-1, the F is chosen2Seedling stage leaf is most purple in plant and florescence most white and 100% test cross infertility the single plant of flower is non-circulation parent This, keeps system for recurrent parent, is returned 1 time, after obtaining backcrossing with the greenery white flower cabbage type rape cytoplasmic male sterility For BC1F1Plant；Again from the backcross progeny BC1F1Selection seedling stage leaf is most purple in plant and the florescence spends most white and 100% test cross not The individual plant selfing educated, obtains BC1F2Plant；C-2, the BC is chosen1F2The single plant that 100% test cross is sterile in plant and seedling stage leaf is most purple and florescence flower is most white is non-circulation Parent keeps system for recurrent parent, is returned 2 times, is returned with the greenery white flower cabbage type rape cytoplasmic male sterility Offspring BC2F1Plant；Again from the backcross progeny BC2F1Selection seedling stage leaf is most purple in plant and the florescence spends most white and 100% test cross The individual plant selfing of infertility, obtains BC2F2Plant；C-3, the BC is chosen2F2The single plant that 100% test cross is sterile in plant and seedling stage leaf is most purple and florescence flower is most white is non-circulation Parent keeps system for recurrent parent, is returned 3 times, is returned with the greenery white flower cabbage type rape cytoplasmic male sterility Offspring BC3F1Plant.
- 4. according to the method described in claim 3, it is characterized by:The strain quantity that the c-1 chooses 100% test cross infertility into c-3 and seedling stage leaf is most purple and florescence flower is most white is 20；Or, choosing BC in the d3F2For 100% test cross infertility in plant and seedling stage leaf is most purple and the florescence spends most white and yield most High strain quantity is 10；Or, hybridizing in the method, being selfed, backcrossing and the place being selfed staggeredly carry out.
- 5. deposit number is that the holding of CGMCC NO.17384 greenery white flower cabbage type rape cytoplasmic male sterility is white flower Shanghai oil 17 Application in the breeding that purple leaf white flower cabbage type rape cytoplasmic male sterility keeps system；Or, the purple leaf white flower cabbage type rape cytoplasmic male sterility holding of claim 1-4 either method breeding ties up to purple leaf Application in the breeding of white flower cabbage type rape cytoplasmic male sterile line；Or, the purple leaf white flower cabbage type rape cytoplasmic male sterility holding of claim 1-4 either method breeding ties up to purple leaf Application in the breeding of white flower Wild cabbage type cytoplasmic male sterility cross-bred rape combination.
- 6. a kind of selection of purple leaf white flower cabbage type rape cytoplasmic male sterile line, be by claim 1-4 either one The purple leaf white flower cabbage type rape cytoplasmic male sterility of method breeding keeps system to carry out Wild cabbage type cytoplasmic male sterility cytoplasm It keeps, obtains purple leaf white flower cabbage type rape cytoplasmic male sterile line.
- 7. according to the method described in claim 6, it is characterized by:Described method includes following steps:It 1) is female parent with the cabbage type rape cytoplasmic male sterility strain, with the purple leaf of claim 1-4 either method breeding It is male parent that white flower cabbage type rape cytoplasmic male sterility, which keeps system, is hybridized, obtains hybrid generation F1；2) from the sterile line hybrid generation F1The middle selection seedling stage leaf most most white and 100% test cross infertility of pale reddish brown phase flower is non-circulation Parent keeps system for recurrent parent, is returned, is returned with the purple leaf white flower cabbage type rape cytoplasmic male sterility Offspring, choosing seedling stage leaf most most white and 100% test cross infertility the strain of pale reddish brown phase flower is purple leaf white flower cytoplasmic male sterile line.
- 8. according to the method described in claim 7, it is characterized by:The backcrossing number is 6 times；And/or to choose from last time backcross progeny, 100% test cross is sterile and seedling stage leaf is most purple and florescence flower for each backcrossing Nonrecurrent parent of the most white plant as backcrossing next time.
- 9. according to method as claimed in claim 6 to 8, it is characterised in that:The cabbage type rape cytoplasmic male sterility strain is cabbage type rape Qin-you No.7 sterile plant.
- 10. a kind of selection of purple leaf white flower Wild cabbage type cytoplasmic male sterility cross-bred rape combination, including following method: will The purple leaf white flower cabbage type rape cytoplasmic male sterile line that any method the method breeding of claim 6-9 obtains Hybridize with common greenery cabbage type rape restorer, realizes purple leaf white flower cabbage type rape cytoplasmic male sterility cross combination Breeding.
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| CN105766626A (en) * | 2016-05-12 | 2016-07-20 | 安徽农业大学 | Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination |
| CN107683764A (en) * | 2017-09-06 | 2018-02-13 | 浙江省农业科学院 | A kind of selection of purple leaf color Chinese cabbage triple crossing kind with a tight waist |
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| CN105766626A (en) * | 2016-05-12 | 2016-07-20 | 安徽农业大学 | Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination |
| CN107683764A (en) * | 2017-09-06 | 2018-02-13 | 浙江省农业科学院 | A kind of selection of purple leaf color Chinese cabbage triple crossing kind with a tight waist |
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