CN107347625A - Strong applications of the female self-mating system LC-03 in pumpkin breeding - Google Patents
Strong applications of the female self-mating system LC-03 in pumpkin breeding Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
Strong applications of the female self-mating system LC 03 in pumpkin breeding.The invention discloses applications of the giant pumpkin LC 03 in pumpkin breeding.Giant pumpkin (Cucurbita maxima) LC 03, preservation registration number are CGMCC NO.12032, also known as strong female self-mating system LC 03.Strong female self-mating system LC 03 as it is maternal with the first female flower florescence of the filial generation of other self-mating systems earlier than its male parent, and female flower interval joint number shows the precocity in 1 generation of hybridization, melon code is close and ties melon growth potential also less than its male parent.It is can be seen that from the strong female height of transformation for self-mating system, effect of increasing production is obvious, therefore the discovery and application of strong female character, to promoting seed to use and meat pumpkin industry development, improving the effect of farmers' income will increasingly show, and the popularizing application prospect of such cross combination is very wide.
Description
Technical field
The present invention relates to applications of the strong female self-mating system LC-03 in pumpkin breeding.
Background technology
Seed-use pumpkin is one of northeast Techniques in Dry-farming Areas major economic crops, as domestic and international market dialogue melon seeds is (general
Referring to the seed of giant pumpkin kind with veriety) demand is continuously increased, and the income of planting household greatly improves, the enthusiasm of plantation
The reason for growing to even greater heights, while the continuous renewal of planting technology, farm machinery is perfect, and promoting cultivated area to expand year by year.
But because China's seed-use pumpkin breeding starting evening, the relative shortage of resource, higher level's department outlay support many reasons such as deficiency to make
The other ground family crops of breeding of new variety Comparision into seed-use pumpkin are relatively lagged behind, and old kind lacks again effectively to be purified
Rejuvenation, to have that regularity is poor, disease resistance weakens, yield is unstable etc. scarce for the conventional variety that largely uses in production at present
Point, can not meet production and the market demand of rapid growth, and peasant household is badly in need of having more precocious, disease-resistant, good quality and high output seeds to use
Pumpkin improved seeds.
Although some existing white melon seeds cenospecies enter market at present, because cenospecies is in the production of hybrid seeds, male flower is removed
Workload is big, cost is high, especially is not easy to observe, emasculation is incomplete during emasculation behind intermediary and later stages cauline leaf envelope ridge of blooming, it is difficult to
Ensure the purity of cenospecies, so as to influence the yield of white melon seeds and quality.How squash hybridization seed seed production purity could be realized
Height, steady quality are reliable, and the production cost for reducing the production of hybrid seeds is the problem that seed-use pumpkin industry is currently badly in need of solving.Practice card
Bright, the amount of labour used, saving labour can both have been reduced by carrying out hybrid seeding using sterile line, female series or strong female series, and and can is abundant
Ensure the purity of cenospecies, have the function that to the grain yield and quality for improving crops important.
The content of the invention
It is an object of the invention to provide applications of the giant pumpkin LC-03 in pumpkin breeding.
Giant pumpkin (Cucurbita maxima) LC-03, is preserved in Chinese microorganism strain on January 25th, 2016
(abbreviation CGMCC, address are preservation administration committee common micro-organisms center：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, in
Institute of microbiology of the academy of sciences of state), preservation registration number is CGMCC NO.12032.Giant pumpkin (Cucurbita maxima)
LC-03CGMCC NO.12032, abbreviation giant pumpkin LC-03, also known as strong female self-mating system LC-03.
Strong female self-mating system LC-03 as it is maternal with the first female flower florescence of the filial generation of other self-mating systems earlier than
Its male parent, and female flower interval joint number shows the precocity in 1 generation of hybridization, melon code is close and ties melon growth potential also less than its male parent.
It can be seen that effect of increasing production is obvious for self-mating system from the strong female height of transformation, therefore the discovery and application of strong female character, to promoting seed
With with meat pumpkin industry development, improving the effect of farmers' income will increasingly show, the popularizing application prospect of such cross combination
It is very wide.The application of strong female character also has clear improvement to characters such as prematureness, bearing fruit property, resistance simultaneously.In seed south
In terms of melon, parent is using More female lines, its big plate rate (transverse diameter is in more than 12.5mm) is that AA level melon seeds has accounted for more than 80%, seed
Regularity and uniformity significantly improve, by per kilogram AA levels melon seeds than common melon seeds can more income 0.5-0.6 member, according to a preliminary estimate only
One index of big plate rate is per 667m2Nearly 50-60 members or so can be increased income.
The purpose of the breeding is the strong female offspring of acquisition.The Progeny plants that the purpose of the breeding increases for acquisition female flower.
The purpose of the breeding is obtains the close Progeny plants of melon code (melon code is close be direct result) more than female flower.The purpose of the breeding
To increase pumpkin yield.
The present invention also protects a kind of pumpkin breeding method, comprises the following steps：It regard strong female self-mating system LC-03 as mother
This, is hybridized with other pumpkins as male parent, obtains F1 generation.Strong female plant in the F1 generation is the target plant of breeding.
The purpose of the breeding is the strong female offspring of acquisition.The Progeny plants that the purpose of the breeding increases for acquisition female flower.The breeding
Purpose to obtain the close Progeny plants of melon code (melon code is close be direct result) more than female flower.The purpose of the breeding is increase south
Melon yield.In F1 generation plant, i.e., it is higher than the plant of the male parent containing female (it is more that female height is embodied as female flower).F1 generation is planted
In strain, i.e., containing the close plant in the male parent of melon code.In F1 generation plant, i.e., it is higher than the plant of the male parent containing yield.F1
The yield in generation is higher than the male parent.
Methods described also comprises the following steps：The F1 generation is selfed, obtains F2 generations.The purpose of the breeding is to obtain
Obtain female offspring by force.The Progeny plants that the purpose of the breeding increases for acquisition female flower.The purpose of the breeding is close to obtain melon code
Progeny plants (melon code is close be direct result) more than female flower.The purpose of the breeding is increase pumpkin yield.F2 in plant,
It is higher than the plant of the male parent containing female (it is more that female height is embodied as female flower).F2 is in plant, i.e., close containing melon code
In the plant of the male parent.F2 is higher than the plant of the male parent in plant containing yield.The yield in F2 generations is higher than the father
This.
Methods described also comprises the following steps：Using the F1 generation as the maternal strong female self-mating system LC- with as male parent
03 is returned, and obtains backcross progeny.The purpose of the breeding is the strong female offspring of acquisition.The purpose of the breeding is acquisition female flower
The Progeny plants increased.The purpose of the breeding for obtain the close Progeny plants of melon code (melon code is close be direct more than female flower after
Fruit).The purpose of the breeding is increase pumpkin yield.In backcross progeny, i.e., it is higher than the plant of the parent sire of hybrid pigs containing female
(it is more that female height is embodied as female flower).In backcross progeny, i.e., containing the close plant in the parent sire of hybrid pigs of melon code.Backcross progeny
In, i.e., containing plant of the yield higher than the parent sire of hybrid pigs.The yield of backcross progeny is higher than the parent sire of hybrid pigs.
Methods described also comprises the following steps：Carried out the F1 generation as maternal other pumpkins with as male parent
Backcrossing, obtains backcross progeny.The Progeny plants that the purpose of the breeding increases for acquisition female flower.The purpose of the breeding is acquisition
The close Progeny plants of melon code (melon code is close be direct result) more than female flower.The purpose of the breeding is increase pumpkin yield.Backcrossing
In offspring, i.e., it is higher than the plant of the parent sire of hybrid pigs containing female (it is more that female height is embodied as female flower).In backcross progeny, i.e.,
Contain the close plant in the parent sire of hybrid pigs of melon code.In backcross progeny, i.e., it is higher than the plant of the parent sire of hybrid pigs containing yield.Return
The yield of offspring is handed over to be higher than the parent sire of hybrid pigs.
The male parent of the hybridization is self-mating system seediness 265, the strong 8-1 of self-mating system or snow city No.1.
The male parent of the backcrossing is strong female self-mating system LC-03, self-mating system seediness 265, the strong 8-1 of self-mating system or snow city one
Number.
A kind of production pumpkin of the present invention also protection or pumpkin submethod, comprise the following steps：Will strong female self-mating system LC-03
As female parent, hybridized with other pumpkins as male parent, obtain F1 generation；Pumpkin or pumpkin are harvested by cultivating the F1 generation
Son.
Methods described also comprises the following steps：The F1 generation is selfed, obtains F2 generations；Withheld by cultivating the F2
Obtain pumpkin or pumpkin seeds.
Methods described also comprises the following steps：Using the F1 generation as the maternal strong female self-mating system with as male parent
LC-03 is returned, and obtains backcross progeny；Pumpkin or pumpkin seeds are harvested by cultivating the backcross progeny.
Methods described also comprises the following steps：Carried out the F1 generation as maternal other pumpkins with as male parent
Backcrossing, obtains backcross progeny；Pumpkin or pumpkin seeds are harvested by cultivating the backcross progeny.
The male parent of the hybridization is self-mating system seediness 265, the strong 8-1 of self-mating system or snow city No.1.
The male parent of the backcrossing is strong female self-mating system LC-03, self-mating system seediness 265, the strong 8-1 of self-mating system or snow city one
Number.
The discovery of strong female series pumpkin resource also shows its prior effect in pumpkin breeding and the production of hybrid seeds etc., main
Superiority is wanted to be embodied in：
1. original seed easily retains and purity is high.Because no matter the strain is selfed, also with the characteristic for having male flower before 10 sections
It is that sister hands over breeding, in the absence of the cumbersome link of the male sterile line three series mating production of hybrid seeds.
2. only being needed once during hybrid seeding emasculation, secondary, emasculation three times can be saved, greatly save emasculation operation into
This, so as to reduce breeding cost.
3. cauline leaf does not seal ridge during due to emasculation, so easily observation, is less prone to the phenomenon that male flower is fallen in no marking, can guarantee that miscellaneous
The quality of the production of hybrid seeds is handed over, purity is high, stablizes high-quality F1 commodity seeds so as to obtain.
4. due to strong female series, the phase can substantially be distinguished before flowering with common pumpkin strain, and now impurity elimination is easy, and
Ensure the important measures of seed purity.
5. although strong female series pumpkin is not dominant in F1 generation, female-flower rate is also significantly more than common pumpkin variety, performance
For female flower appearance is early, seat melon is easy, yield potential is huge.
6. in the F2 generations that strong female series prepare combination, can show the strong female strain in part, or be backcrossing parent with strong female pumpkin
This, first backcross generation, strong female plants just show more than 50%, it is possible to the hereditability using this characteristic, cultivate more
Good strong female strain is used for the breed improvement and production of other pumpkins.
Embodiment
Following embodiment facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method, it is conventional method unless otherwise specified.Test material used in following embodiments, it is certainly unless otherwise specified
What routine biochemistry reagent shop was commercially available.Quantitative test in following examples, it is respectively provided with and repeats to test three times, as a result make even
Average.
Self-mating system seediness 265：Heilungkiang Baoqing County means of agricultural production shop.The strong 8-1 of self-mating system：Heilungkiang Baoqing County means of agricultural production shop." Huanan
Without wooden fork "：Heilungkiang Baoqing County means of agricultural production shop.Avenge city No.1 (Mei Yaxue cities)：Fujin City plum subspecies industry Co., Ltd of Heilongjiang Province.From
Friendship is that seediness 265, the strong 8-1 of self-mating system, " Huanan is without wooden fork " and snow city No.1 are pumpkin.
The acquisition of embodiment 1, strong female self-mating system LC-03
In Zhenhua from Heilungkiang Baoqing County farmers' strain a polygeny product are filtered out in earlier 1990s
System, named through inbreeding of more generation purification as seediness 265.The characteristics of seediness 265：Female-flower rate is especially high, and it is individual female that 2-3 occurs in an axil
Colored ratio is higher, and more toward the section position on plant top, female flower ratio is higher, but still has male flower appearance；Melon be celadon skin,
Oblateness, seed white, single melon produce seed 250 or so.
There is one plant of variation for self-mating system in the high of seediness 265 in 2003, before the section of the variant root 10 raw male flower
And hermaphrodite flower, afterwards every axil raw female flower, no longer there is male flower, for this characteristic, carry out individual plant selfing pollination, together
Year September end harvests ripe one, melon.Sow 150 plants within 2004, show as strong female plant and account for 76%, remove different from breeding goal
After non-strong female plant, selfing purification is carried out every year to the individual plant for showing as strong female plant, through 4 years 5 generations (Hainan adds a numerous generation) seed selection
Go out more stable strong female self-mating system, be named as giant pumpkin LC-03.
Giant pumpkin (Cucurbita maxima) LC-03, is preserved in Chinese microorganism strain on January 25th, 2016
(abbreviation CGMCC, address are preservation administration committee common micro-organisms center：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, in
Institute of microbiology of the academy of sciences of state), preservation registration number is CGMCC NO.12032.Giant pumpkin (Cucurbita maxima)
LC-03CGMCC NO.12032, abbreviation giant pumpkin LC-03, also known as strong female self-mating system LC-03.
Strong female self-mating system LC-03 belongs to Curcurbitaceae Cucurbita giant pumpkin kind, and major traits are as follows：
(1) growing way is medium, long climing, climing long 3-4m, branch 2-3；
(2) blade is rounded, light green color, incise it is shallow, at every axil a raw tendril；
(3) before major branch 8-10 sections raw 8-12 male flower (including hermaphrodite flower), start female flower occur after 8-10 sections, from the
After one female flower occurs, per blade axil at raw female flower, female anthocaulus is longer, on calyx piece raw 5-6 small needles, side shoot is only
Raw female flower；
(4) tender melon melon color is white green and green in light gray, the white slightly ash of ripe melon and green, subcircular or oblateness,
Single melon weighs 3~4kg；
(5) 100~150 grams of seed of single melon production, seed production are about 70kg/667 ㎡ or so, mass of 1000 kernel 350-400g or so,
The average horizontal stem of seed × vertical stem is about 1.10~1.25 × 2.10~2.30cm or so, and seed kind skin is white, seed edge very little
Or nothing.
The application of embodiment 2, strong female self-mating system LC-03 (male parent is used as using self-mating system seediness 265)
First, pumpkin breeding is carried out using strong female self-mating system LC-03
1st, 2007, authorize the pollen of self-mating system seediness 265 to strong female self-mating system LC-03, normal management, obtain seed
(F1 generation).
2nd, 2008, the seed that plantation step 1 obtains, the pollen of male flower is authorized to female flower, normal management and continuous observation
Growing state, harvest seed (F2 generations).
2nd, character analysis
1st, in May, 2008, in Heilungkiang Baoqing County, sowing test seed, normal management and continuous observation growing state.Examination
It is respectively F1 generation (seed), strong female self-mating system LC-03 (seed), self-mating system seediness 265 (seed) to test seed, carries out parallel place
Reason.
2nd, in October, 2008, in Hainan Province, sowing test seed, normal management and continuous observation growing state.Experiment kind
Son is respectively " Huanan is without wooden fork " (seed), strong female self-mating system LC-03 (seed), self-mating system seediness 265 (seed), F1 generation (kind
Son) and F2 generations (seed), carry out parallel processing.
4th, in May, 2009, in Heilongjiang Province, sowing test seed, normal management and continuous observation growing state.Experiment kind
Son is respectively " Huanan is without wooden fork " (seed), strong female self-mating system LC-03 (seed), self-mating system seediness 265 (seed), F1 generation (kind
Son) and F2 generations (seed), carry out parallel processing.
5th, the comparison of parent and the average section position residing for F1 generation female flower
The statistical result of average section position residing for the first female time in parent's each time and female flower is shown in Table 1, and (each time is every
Kind parent counts the average value of 100 plants of plant).Average section position residing for first female time in F1 generation each time and first female flower
Statistical result is shown in Table 2 (each time counts the average value of 100 plants of plant).Strong female self-mating system LC-03 is average in 8.4 leaves
There is first female flower, the female flower of latter leaf one, occur without male flower.Self-mating system seediness 265 is average to occur head in 10.2 leaves
Female flower, later at interval of 5.6 leaves when occur female flower again.Strong female self-mating system LC-03 obtains with the hybridization of self-mating system seediness 265
F1 generation, it is average to occur first female flower in 9.7 leaves, later at interval of 5.3 leaves when occur female flower again.The first female time is grown
Go out first female time spent.1 female i.e. first female flower.2 female i.e. second female flower.3 female i.e. the 3rd female flowers.4 the female i.e. the 4th
Piece female flower.
Table 1
Table 2
6th, the ratio of F2 different type plant in
The definition of strong female plant：Counted since root, after the n-th 1 sections every axil raw female flower, no longer there is male flower,
N1 is less than 10 natural number.The definition of half strong female plant：Counted since root, after the n-th 2 sections every axil raw female flower,
No longer there is male flower, n2 is natural number and 15≤n2≤25.The definition of more female plants：Phenotype without strong female plant and without half
The phenotype of strong female plant, more than continuous 4 section go out female flower and/or a certain save out more than 4 female flowers.The definition of normal strain：It is not belonging to
State three kinds of situations for normal plant.
Based on above-mentioned standard, F2 is shown in Table 3 (each time counts 20 plants of plant) for the separation situation of plant.
Heilungkiang in 2009, the Relevant phenotype in 20 plants of F2 generations are shown in Table 4.Strain number is female by force for 3,4,10,13,14 and 20 plant
Strain, accounts for 30%.Strain number is the last half female plant for 1,11 plant, accounts for 10%.Strain number is more for 7,9,12,16,17 and 18 plant
Female plant, account for 30%.Strain number is normal strain for 2,5,6,8,15 and 19 plant, accounts for 30%.
As a result show, F2 generation there is female flower section position, the phenomenon that Female Flower Number is clearly separated.
Table 3
Table 4
7th, the yield of F1 generation
The yield of the F1 generation in each time is shown in Table 5, and (data in table 5 are the average statistics data of three cells, each cell
=6 rows × 10m × 0.68m=40.8 ㎡).Seed be relatively shown in Table 6 (data in table 6 for three cells average statistics data,
Each cell=6 rows × 10m × 0.68m=40.8 ㎡).
Table 5
Table 6
Compared with self-mating system seediness 265, the F1 generation and F2 cultivated under parallel condition increase for equal female flower, corresponding melon code
It is close, so as to yield increase.
The application of embodiment 3, strong female self-mating system LC-03 (using snow city No.1 or the strong 8-1 of self-mating system as male parent)
First, first hybridize and be selfed again (using snow city No.1 as male parent)
Self-mating system seediness 265 is replaced with snow city No.1, other are the same as embodiment 2.
It is (every that the statistical result of average section position residing for the first female time in each parent's each time and first female flower is shown in Table 7
Individual time every kind of parent counts the average value of 100 plants of plant).It is flat residing for the first female time in F1 generation each time and first female flower
The statistical result for saving position is shown in Table 8 (each time counts the average value of 100 plants of plant).Strong female self-mating system LC-03 averagely exists
Occur first female flower during 8.4 leaves, the female flower of latter leaf one, occur without male flower.Snow city No.1 is average to be occurred in 8.2 leaves
First female flower, later at interval of 5.2 leaves when occur female flower again.Strong female self-mating system LC-03 obtains with snow city No.1 hybridization
F1 generation, it is average the first female flower occur in 7.5 leaves, later at interval of 4.8 leaves when occur female flower again.
Table 7
Table 8
F2 is shown in Table 9 (each time counts 20 plants of plant) for the separation situation of plant.
Table 9
The yield of the F1 generation in each time is shown in Table 10, and (data in table 5 are the average statistics data of three cells, each cell
=6 rows × 10m × 0.68m=40.8 ㎡).Seed is relatively shown in Table 11, and (data in table 6 are the average statistics number of three cells
According to each cell=6 rows × 10m × 0.68m=40.8 ㎡).
Table 10
Table 11
Compared with avenging city No.1, the F1 generation and F2 cultivated under parallel condition increase for equal female flower, and corresponding melon code is close, so as to
Yield increase.
2nd, first hybridize and be selfed again (using the strong 8-1 of self-mating system as male parent)
Self-mating system seediness 265 is replaced with the strong 8-1 of self-mating system, other are the same as embodiment 2.
The statistical result of average section position residing for the first female time in each parent's each time and female flower was shown in Table for 12 (each years
The every kind of parent of part counts the average value of 100 plants of plant).Average section residing for the first female time in F1 generation each time and first female flower
The statistical result of position is shown in Table 13 (each time counts the average value of 100 plants of plant).Strong female self-mating system LC-03 is average 8.4
Occur first female flower during piece leaf, the female flower of latter leaf one, occur without male flower.The strong 8-1 of self-mating system is average to be gone out in 10.1 leaves
Existing head female flowers, later at interval of 5.1 leaves when occur female flower again.Strong female self-mating system LC-03 8-1 hybridization strong with self-mating system obtains
F1 generation, it is average the first female flower occur in 9.3 leaves, later at interval of 4.8 leaves when occur female flower again.
Table 12
Table 13
F2 is shown in Table 14 (each time counts 20 plants of plant) for the separation situation of plant.
Table 14
The yield of the F1 generation in each time is shown in Table 15, and (data in table 5 are the average statistics data of three cells, each cell
=6 rows × 10m × 0.68m=40.8 ㎡).Seed is relatively shown in Table 16, and (data in table 6 are the average statistics number of three cells
According to each cell=6 rows × 10m × 0.68m=40.8 ㎡).
Table 15
Table 16
Compared with the strong 8-1 of self-mating system, the F1 generation and F2 cultivated under parallel condition increase for equal female flower, and corresponding melon code is close,
So as to yield increase.
3rd, first hybridize and be returned again
Scheme 1：(strong female self-mating system LC-03 × snow city No.1) × strong female self-mating system LC-03；I.e. strong female self-mating system
LC-03 as the snow city No.1 of male parent as maternal with hybridizing to obtain F1 generation, and F1 generation is as the maternal strong female with as male parent
Self-mating system LC-03 is returned；
Scheme 2：(strong female self-mating system LC-03 × strong 8-1 of self-mating system) × strong female self-mating system LC-03；I.e. strong female is certainly
Friendship is that LC-03 hybridizes to obtain F1 generation as maternal and as male parent the strong 8-1 of self-mating system, and F1 generation is as female parent and as male parent
Strong female self-mating system LC-03 backcrossings；
Scheme 3：(strong female self-mating system LC-03 × snow city No.1) × snow city No.1；I.e. strong female self-mating system LC-03 conducts
Maternal and as male parent snow city No.1 hybridizes to obtain F1 generation, and F1 generation is returned as the maternal snow city No.1 with as male parent；
Scheme 4：(strong female self-mating system LC-03 × strong 8-1 of self-mating system) strong 8-1 of × self-mating system；I.e. strong female self-mating system LC-
03 hybridizes to obtain F1 generation as maternal and as male parent the strong 8-1 of self-mating system, and F1 generation is as the maternal self-mating system with as male parent
Strong 8-1 backcrossings.
" (strong female self-mating system LC-03 × snow city No.1) × strong female self-mating system LC-03 ", " (strong female self-mating system LC-
The strong 8-1 of 03 × self-mating system) in the backcross progenies that obtain of × strong female self-mating system LC-03 ", female flower section rate shows as normal strain：By force
Female plant is close to 1：1." (strong female self-mating system LC-03 × snow city No.1) × snow city No.1 ", " (strong female self-mating system LC-03 × from
Friendship is strong 8-1) in the backcross progenies that obtain of the strong 8-1 " of × self-mating system, female flower section rate shows same backcross parent, does not occur strong female
Strain.
Correlated results in step 2 and step 3.In filial generation, female flower section position trait expression tends to male parent material, does not have
There are the typical properties and characteristicses for showing strong female maternal female flower section position.F2 shows obvious separation, segregation ratio for female flower section rate
Example is normal strain：Strong female plant ≈ 3：1.
With reference to the above results, it can be initially believed that female character is a pair of relativity to the normal character pair of female flower section rate by force, by
A pair of Relative gene control, wherein strong female character is recessive character, normal female flower section rate is dominant character.In the practices of breeding,
It was found that the section position height of female flower, quantity are simultaneously also very big by such environmental effects, therefore the quantity that female flower also can be explained can
Also it can be controlled by multiple minor genes.
Claims (9)
1. applications of the giant pumpkin LC-03 in pumpkin breeding；The giant pumpkin LC-03 is that preservation registration number is CGMCC
NO.12032 giant pumpkin (Cucurbita maxima) LC-03.
2. a kind of pumpkin breeding method, comprises the following steps：Using giant pumpkin LC-03 as female parent, and as the other of male parent
Pumpkin is hybridized, and obtains F1 generation；The giant pumpkin LC-03 is the giant pumpkin that preservation registration number is CGMCC NO.12032
(Cucurbita maxima)LC-03。
3. method as claimed in claim 2, it is characterised in that：Methods described also comprises the following steps：The F1 generation is carried out
Selfing, obtains F2 generations.
4. method as claimed in claim 2, it is characterised in that：Methods described also comprises the following steps：Using the F1 generation as
Female parent is returned with the giant pumpkin LC-03 as male parent, obtains backcross progeny.
5. method as claimed in claim 2, it is characterised in that：Methods described also comprises the following steps：Using the F1 generation as
Female parent is returned with other pumpkins as male parent, obtains backcross progeny.
6. one kind production pumpkin or pumpkin submethod, comprise the following steps：Using giant pumpkin LC-03 as female parent, and as father
This other pumpkins are hybridized, and obtain F1 generation；Pumpkin or pumpkin seeds are harvested by cultivating the F1 generation；The giant pumpkin
LC-03 is giant pumpkin (Cucurbita maxima) LC-03 that preservation registration number is CGMCC NO.12032.
7. method as claimed in claim 6, it is characterised in that：Methods described also comprises the following steps：The F1 generation is carried out
Selfing, obtains F2 generations；Withheld by cultivating the F2 and obtain pumpkin or pumpkin seeds.
8. method as claimed in claim 6, it is characterised in that：Methods described also comprises the following steps：Using the F1 generation as
Female parent is returned with the giant pumpkin LC-03 as male parent, obtains backcross progeny；Received by cultivating the backcross progeny
Obtain pumpkin or pumpkin seeds.
9. method as claimed in claim 6, it is characterised in that：Methods described also comprises the following steps：Using the F1 generation as
Female parent is returned with other pumpkins as male parent, obtains backcross progeny；By cultivating the backcross progeny harvest south
Melon or pumpkin seeds.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112586344A (en) * | 2020-12-09 | 2021-04-02 | 湖南人文科技学院 | Breeding method of pumpkin of male sterile dual-purpose line GMS-Ma1802 |
| CN113179944A (en) * | 2020-12-07 | 2021-07-30 | 湖南人文科技学院 | Application of waterlogging-resistant melon M13-6-3 in melon breeding |
| CN113287516A (en) * | 2021-06-11 | 2021-08-24 | 上海曹野农业发展有限公司 | Fruit pumpkin polymerization breeding method taking fruit indexes as breeding standards |
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| WO2009085982A1 (en) * | 2007-12-19 | 2009-07-09 | Monsanto Technology Llc | Method to enhance yield and purity of hybrid crops |
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| CN113179944A (en) * | 2020-12-07 | 2021-07-30 | 湖南人文科技学院 | Application of waterlogging-resistant melon M13-6-3 in melon breeding |
| CN112586344A (en) * | 2020-12-09 | 2021-04-02 | 湖南人文科技学院 | Breeding method of pumpkin of male sterile dual-purpose line GMS-Ma1802 |
| CN113287516A (en) * | 2021-06-11 | 2021-08-24 | 上海曹野农业发展有限公司 | Fruit pumpkin polymerization breeding method taking fruit indexes as breeding standards |
| CN113287516B (en) * | 2021-06-11 | 2023-08-25 | 上海曹野农业发展有限公司 | Fruit pumpkin polymerization breeding method taking fruit index as breeding standard |
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| CN107347625B (en) | 2019-11-12 |
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