CN106804423A - A kind of breeding method of white glume barley variety - Google Patents
A kind of breeding method of white glume barley variety Download PDFInfo
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- CN106804423A CN106804423A CN201710034379.1A CN201710034379A CN106804423A CN 106804423 A CN106804423 A CN 106804423A CN 201710034379 A CN201710034379 A CN 201710034379A CN 106804423 A CN106804423 A CN 106804423A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The present invention is a kind of breeding method of white glume barley variety, belongs to field of plant variety breeding technology.Make male parent for No. 3 with malting barley variety salt wheat, make maternal with malting barley variety Supi 3, carry out artificial hybridization combo, F1In generation, carries out Anther Culture and EMS mutagenic treatments, the selection of the appearance character of continuous multi-generation, yield is carried out to its mutagenic treatment offspring, it is white, yield level high, disease-resistant to cultivate heading to glume and auricle during being in the milk, the ripe phase is moderate, wide adaptability, the excellent characteristic New Barley Variety salt 08036 of Comprehensive Traits.
Description
First, technical field
The present invention is a kind of breeding method of white glume barley variety, belongs to field of plant variety breeding technology.More specifically
Say it is to be exclusively used in screening special barley variety.
2nd, background technology
Barley is the fourth-largest cereal crop in the world, and planting range is wide, integrate brewage, it is feeding and edible, nearly tens
Nian Lai, world's barley sown area and total output are fastest-rising in cereal crops, and average growth rate is up to more than 80%.China
Although starting evening, germ plasm resource lacks, instrument and equipment and breeding technique fall behind for barley variety improvement work, but by a large amount of introductions
External high yield and high quality germ plasm resource and the unremitting effort of breeder, Barley Breeding level is progressively close to foreign countries' last century nine
The ten's level.Jiangsu is one of main producing region of China's malting barley, and since Eleventh Five-Year Plan, Jiangsu Province's Barley Breeding work is obtained
Remarkable break-throughs, Barley Breeding level maintains the leading position at home, successively selects a collection of high yield, high-quality, disease-resistant barley
New varieties, are the raising of China's barley production, are played the important and pivotal role.Since the end of last century, with people's lives
Level being enriched constantly and improving, and the Beer Industry of China has obtained fast development, as the first Beer Brewage in the world and disappears
Take big country, malting barley raw material more than 60% relies on import.Meanwhile, developing rapidly for animal husbandry also increases feed demand amount,
Barley is also preferable feedstuff.This requires domestic Barley Breeding and production work has to comply with actual demand, and progressively
Meet the breach of beer and Feed Manufacturing.However, the market management of confusion has a strong impact on and upset normal Seed Market, lead
Regular Seed enterprises and breeder is caused to sustain a great loss.If there is obvious and typical area in kind appearance characteristics feature
Not, it will help the Rapid identification of true and false seed, the legitimate rights and interests of protection Seed enterprises and breeder, so as to protect peasant not receive
Leading into a trap for fake and forged seed, it is overall to improve the barley level of production.
3rd, inventive technique content
Technical problem
The present invention is in existing barley variety seed selection, and barley variety growth period appearance character is not notable, distinguishes and differentiates tired
Difficulty, Seed Market personation deck kind is more to be had a strong impact on normal production, manages, sells, and is caused seriously to breeder, peasant
A kind of the problems such as economic loss, there is provided breeding method of white glume barley variety, cultivate and Screening and Identification goes out to adapt in the Yellow River and Huai He River
Area growth, white glume barley variety.
Technical scheme
The present invention provides a kind of breeding method of white glume barley variety, comprises the following steps：
Make male parent for No. 3 with salt wheat, Supi 3 makees maternal, carries out sexual hybridization, sexual hybridization F1Spend at training and EMS
It is the M of CGMCC No.12660 that preserving number is obtained after reason2The barley M20080805 of platinite glume, is carried out self propagated,
Selection：Plant type is compact, plant height 67-80cm, the 208-213 days time of infertility, base portion Trunk diameter 0.25-0.35cm, and length is saved under fringe
13.5-15.0cm, spike length 6.0-7.0cm, heading to glume before maturation and auricle are white, by planting mirror in selective nursery
It is fixed, obtain M3The white glume fine individual plant in generation.
Gained is stated into M3The selfing again of the fine individual plant in generation, 2 row areas/strain, line-spacing 30cm, spacing in the rows 2cm enter in selective nursery
Row selects M4For yield with to compare Supi 3 close, economical character requirement plant height 67-80cm, plant type are compact, grain husk before heading to maturation
Shell and auricle are white, the white glume individual plant of the effective fringe 52-62 of mu ten thousand.
Selected and remain after carrying out selection individual plant in the selective nursery, then during species test indoors the individual plant of more than mass of 1000 kernel 36g, directly
To M4In generation, has been bred as white glume barley variety.
A kind of breeding method of white glume barley variety, be bred as white glume New Barley Variety to name is salt
08036, the varietal characteristic is：The kind semi-winterness, seedling half is upright, and lowest order leaf leaf sheath is without fine hair, and leaf color is green, and heading is extremely
Glume and auricle are white before ripe, the effective fringe 52-62 of mu ten thousand, and heading stage is close with Supi 3, and pain phase 207- is given birth to entirely
212 days；Fringe appearance is upright, and the wheat head is in two ribs, fringe turriform, spike length 6.0-7.0cm, Defined daily doses 24-30, mass of 1000 kernel 36-
40g。
Beneficial effect
The present invention processes the white glume barley variety salt 08036 of continuous multi-generation selfed breeding by Anther Culture and EMS,
The kind semi-winterness, seedling half is upright, and lowest order leaf leaf sheath is without fine hair, and leaf color is green, and heading to glume before maturation and auricle is
White, boot leaf is hung down loosely, and stooling is stronger, and the percentage of earbearing tiller is high, the general effective fringe 52-62 ten thousand of mu, and heading stage is close with Supi 3,
The complete raw 208-213 days pain phase；Awns is more long and spinosity, awns tip very slight color, closes grain husk and blooms, and fringe appearance is upright, and the wheat head is in two ribs, fringe
Turriform, spike length 6.0-7.0cm, Defined daily doses 24-30, mass of 1000 kernel 36-40g.
The white glume barley variety salt 08036 that the present invention is cultivated has the following advantages that and good effect：
1st, yield is high, stable, and yield potential is big
This institute of the participation of salt 08,036 2013~2014 the new strain of barley qualification test, 478.4 kgs/acre of average product, than
Control Supi 3 is low by 1.2%, participates within 2014~2015 years the new strain of barley comparative test of this institute, and 492.3 kilograms of average product/
Mu, than control Supi 3 volume increase 0.8%.The variety yield is substantially close with Supi 3, and yield level is relatively stable, demonstration examination
Test and experiment in cultivation result shows：The average yield per mu level of salt 08036 is 450~500 kilograms, and yield potentiality is up to per mu yield 500
~530 kilograms.Spread, can not only effectively alleviate domestic beer and use and feeding breach pressure, while can also increase peasant
Income, creates social benefit.
2nd, varietY specificity is obvious
Salt 08036 is to make male parent by No. 3 with salt wheat, and Supi 3 makees maternal, sexual hybridization carried out, in its F1In generation, is carried out
Anther Culture and EMS treatment, select and stablize by many generations, the special barley variety of cultivation.The appearance characteristics of the kind are notable,
Before showing as heading stage to maturation, glume and auricle are white, hence it is evident that be different from other barley varieties.This feature is the kind
Identity business card, false proof anti-false effect can be effectively played.
3rd, with preferable ornamental value
Before heading to ripe annesl, glume and auricle position show to be different from the kind due to the missing of chlorophyll
The white of other barley varieties, sight is preferably.There can be preferable ornamental value as a kind of ornamental plant.
4th, biological deposits
White glume barley M20080805, Classification And Nomenclature is two rib barleys (Hordeum distichon), in 2016
, on August be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center for 22, and address Chaoyang District, Beijing City is northern
The institute 3 of occasion West Road 1, institute of microbiology of the Chinese Academy of Sciences, preserving number is：CGMCC No.12660.
5th, specific embodiment
White glume barley variety breeding method of the invention is further illustrated below in conjunction with accompanying drawing.
A) male parent is made with salt wheat No. 3, Supi 3 makees maternal, carries out sexual hybridization, obtains F0For seed.
B) the F of above-mentioned acquisition0For seed, carry out planting to obtain F1For plant, F is taken1Monokaryon middle and advanced stage is in for pollen development
Tassel carries out Anther Culture, after the callus of Anther Culture is soaked 3 hours in 0.4% EMS solution, aseptic flushing,
It is inoculated in inducing culture, differential medium culture is transferred to after 5 days.By obtaining M after EMS treatment121 plants of Dai Lvmiao.
C) the M that will be obtained121 plants of green seeds of mutagenesis of generation are planted in crop field, and selfing harvests M1For seed, by M1Fringe is planted for seed
OK, row 2m, line-spacing 30cm long, in Breeding Process, in M2Find that glume and auricle are during finding heading to booting in generation white
1 plant of the individual plant of color, is marked, and maturation harvests M2For single-strain seed.After results, by the individual plant M of white2For Seed Deposit, preservation
Number it is:CGMCC No.12660.
D) the white glume M that will be harvested2Self propagated is carried out for seed, the plant of selection traits stabilization is further identified.
Selection：White glume and auricle, plant type are compact, 67~80cm of plant height, 208~213 days time of infertility, base portion Trunk diameter
13.5-15.0cm long is saved under 0.25-0.35cm, fringe, heading to glume before maturation and auricle is the M of white3White glume is excellent
Good individual plant.
E) and then in selfing select, 2 row areas/strain, line-spacing 30cm, spacing in the rows 2cm, from M3In generation, starts, by generation in seed selection
Glume and auricle color, plant type, fringe color, leaf color Identification, Yield comparison, economical character and individual plant after results are carried out in garden
The selection of shape.Yield is similar with Supi 3 (control), and economical character requirement plant height 67-80cm, plant type are compact, the effective fringe 52 of mu
~62 ten thousand, heading to glume before maturation and auricle is white；Carry out artificial threshing after results again indoors again, selected and remain during species test
Grain plumpness is good；The individual plant that more than 36 grams of mass of 1000 kernel；The fine individual plant that reservation meets breeding objective enters next breeding generation
In generation, carries out cultivation work.From M1In generation, starts, and white glume barley variety salt 08036 has been bred as after the generation of selfing 4.
Subordinate list：The choosing of educating of white glume barley variety salt 08036 of the invention is passed through
The white cultural technigue of glume barley variety salt 08036：
(1) elite seed：Seed cleaning is carried out before sowing, it is ensured that purity more than 99%, cleanliness more than 95%, selected mistake
Strictly controlled in journey, prevent mechanical admixture, percentage of seedgermination from must reach more than 85%.
(2) arrange field, begin sowing in good time：Before jiangsu coast Saline Region, plantation barley, it is necessary to carry out field whole
Reason, keeps field face smooth.After plantation, discharge conduit is excavated, the channel depth will be wanted in 30~40cm or so, discharge conduit networking
It is close, to ensure that pouring salt salt discharge of the rainfall to soil is acted on, mitigate stress of the soil salt to barley growth.Broadcast on salinization soil
Kind of time should on October 23 between November 5, the desalination soil upper sowing time select be within 15th to November on October 28
Preferably.
(3) rational close planting：In Yancheng Littoral Zone, Jiangsu Province, under conditions of proper seeding, Basic Seedling is with 18 on salinization soil
Ten thousand or so are advisable, and sowing date for early sowing or fertility level are higher, the relatively low field of soil salt content, and application rate can reduce by 10~
20%.Desalination soil field Basic Seedling is advisable with 160,000, and sowing is late or the poor visual concrete condition of fertility suitably increases Basic Seedling,
Guarantee has certain colony.
(4) Optimum：According to Growth and Development of Barley rule and the feature of salt 08036, before the principle of fertilising should be
Rush, middle control, rear benefit, base manure may advantageously facilitate wheat seeding morning tiller enough, and stem tiller number reaches 50~550,000, the control of highest stem tiller before making the winter
Below 1,100,000, suitably small amount supplement nitrogenous fertilizer, extends the functional period of blade after jointing, increases yield.In 400 kilograms of yield
Under level, optimum nitrogen rate is 25~30 purity nitrogens, and wherein base manure accounts for 70% or so, topdresses and account for 30%, in 2~3 ‰ salt marsh
Change on soil, nitrogenous fertilizer 30kg/ mus, phosphate fertilizer 20kg/ mus, on desalination soil, nitrogenous fertilizer 35kg/ mus, phosphate fertilizer 25kg/ mus is big optimum
The growth of wheat.
(5) harvest in good time：When salt 08036 has 30% tassel to bend, you can harvest, early receipts influences its germination percentage.And and
When threshing, ted and put in storage, can't stacking, influence wheat germination percentage and its seed color and luster, it is ensured that seed have hair higher
Bud rate, improves its commodity.
Claims (5)
1. a kind of breeding method of white glume barley variety, including：
Make male parent for No. 3 with salt wheat, Supi 3 makees maternal, sexual hybridization F1Acquisition preserving number is after carrying out spending training and EMS treatment
The M of CGMCC No.126602Platinite glume barley M20080805, is carried out self propagated, selection：Plant type is compact, plant height
67-80cm, the 208-213 days time of infertility, base portion Trunk diameter 0.25-0.35cm saves 13.5-15.0cm long, spike length under fringe
6.0-7.0cm, heading to glume before maturation and auricle is white, by the field plot test in selective nursery, obtains M3Platinite
The fine individual plant of glume.
2. a kind of selection of white glume barley variety according to claim 1, including：
By M described in claim 13The selfing again of the fine individual plant in generation, 2 row areas/strain, line-spacing 30cm, spacing in the rows 2cm, in selective nursery
Carry out selection M4For yield with to compare Supi 3 close, economical character requirement plant height 67-80cm, plant type are compact, heading to maturation
Preceding glume and auricle are white, the white glume individual plant of the effective fringe 52-62 of mu ten thousand.
3. the selection of a kind of white glume barley variety according to claim 1 or claim 2, including：
Select and remain more than mass of 1000 kernel 36g after carrying out selection individual plant in selective nursery described in claim 1 or 2, then during species test indoors
Individual plant, until M4In generation, has been bred as white glume barley variety.
4. a kind of breeding method of white glume barley variety according to claim 1 or claim 2, is bred as white glume barley new product
It is salt 08036 that kind is named, and the varietal characteristic is：
The kind semi-winterness, seedling half is upright, and lowest order leaf leaf sheath is without fine hair, and leaf color is green, heading to glume before maturation and auricle
It is white, the effective fringe 52-62 of mu ten thousand, heading stage is close with Supi 3, the complete raw 207-212 days pain phase；Fringe appearance is upright, wheat
Fringe is in two ribs, fringe turriform, spike length 6.0-7.0cm, Defined daily doses 24-30, mass of 1000 kernel 36-40g.
5. a kind of breeding method of white glume barley variety according to claim 3, is bred as white glume New Barley Variety
It is salt 08036 to name, and the varietal characteristic is：
The kind semi-winterness, seedling half is upright, and lowest order leaf leaf sheath is without fine hair, and leaf color is green, heading to glume before maturation and auricle
It is white, the effective fringe 52-62 of mu ten thousand, heading stage is close with Supi 3, the complete raw 207-212 days pain phase；Fringe appearance is upright, wheat
Fringe is in two ribs, fringe turriform, spike length 6.0-7.0cm, Defined daily doses 24-30, mass of 1000 kernel 36-40g.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997688A (en) * | 2019-05-10 | 2019-07-12 | 浙江省农业科学院 | The New Barley Variety method for integrate ornamental and feeding is cultivated in a kind of chemical mutagenesis |
| CN112369320A (en) * | 2020-12-02 | 2021-02-19 | 盐城师范学院 | Breeding method of multi-edge hybrid barley |
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2017
- 2017-01-17 CN CN201710034379.1A patent/CN106804423B/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997688A (en) * | 2019-05-10 | 2019-07-12 | 浙江省农业科学院 | The New Barley Variety method for integrate ornamental and feeding is cultivated in a kind of chemical mutagenesis |
| CN112369320A (en) * | 2020-12-02 | 2021-02-19 | 盐城师范学院 | Breeding method of multi-edge hybrid barley |
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Address after: No. 59 Open Avenue, Yancheng City, Jiangsu Province, 224002 Patentee after: Jiangsu Inst. of Coastal Area Agricultural Sciences Address before: 224002 No. 59 Open Avenue, Huaian City, Jiangsu Province Patentee before: Jiangsu Inst. of Coastal Area Agricultural Sciences |