CN106804423B - A kind of breeding method of white glume barley variety - Google Patents
A kind of breeding method of white glume barley variety Download PDFInfo
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- CN106804423B CN106804423B CN201710034379.1A CN201710034379A CN106804423B CN 106804423 B CN106804423 B CN 106804423B CN 201710034379 A CN201710034379 A CN 201710034379A CN 106804423 B CN106804423 B CN 106804423B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The present invention is a kind of breeding method of white glume barley variety, belongs to field of plant variety breeding technology.Make male parent for No. 3 with malting barley variety salt wheat, makees maternal, progress artificial hybridization combo, F with malting barley variety Supi 31In generation, carries out Anther Culture and EMS mutagenic treatment, the selection of the appearance character, yield of continuous multi-generation is carried out to its mutagenic treatment offspring, cultivating heading to glume and auricle during being in the milk is that white, yield level are high, disease-resistant, the ripe phase is moderate, wide adaptability, the excellent characteristic New Barley Variety salt 08036 of Comprehensive Traits.
Description
One, technical field
The present invention is a kind of breeding method of white glume barley variety, belongs to field of plant variety breeding technology.More specifically
Say it is to be exclusively used in screening special barley variety.
Two, background technique
Barley is the fourth-largest cereal crop in the world, and planting range is wide, integrates brewing, feeding and edible, nearly tens
Nian Lai, world's barley sown area and total output be it is fastest-rising in cereal crops, average growth rate is up to 80% or more.China
Barley variety improves work although starting evening, and germ plasm resource lacks, instrument and equipment and breeding technique fall behind, but by largely introducing
The unremitting effort of external high yield and high quality germ plasm resource and breeder, Barley Breeding level is gradually close to external last century nine
The ten's are horizontal.Jiangsu is one of the main producing region of China's malting barley, and since Eleventh Five-Year Plan, Barley Breeding work in Jiangsu Province's is obtained
Remarkable break-throughs, Barley Breeding level maintain the leading position at home, successively select a collection of high yield, high-quality, disease-resistant barley
New varieties are the raising of China's barley production, play the important and pivotal role.Since the end of last century, with people's lives
Horizontal enriches constantly and improves, and the Beer Industry in China is grown rapidly, and becomes the first Beer Brewage in the world and disappears
Take big country, 60% or more malting barley raw material relies on import.Meanwhile the rapid development of animal husbandry, also feed demand is increased,
Barley is also preferable feedstuff.This requires domestic Barley Breeding and production works to have to comply with actual demand, and gradually
Meet the notch of beer and Feed Manufacturing.However, chaotic market management seriously affects and upset normal Seed Market, lead
Regular Seed enterprises and breeder are caused to sustain a great loss.If there is obvious and typical area in kind appearance characteristics feature
Not, it will help the Rapid identification of true and false seed protects the legitimate rights and interests of Seed enterprises and breeder, thus protect peasant not by
Fake and forged seed is led into a trap, whole to improve barley production level.
Three, inventive technique content
Technical problem
For the present invention in existing barley variety breeding, barley variety growth period appearance character is not significant, distinguishes to identify and be stranded
Difficulty, Seed Market personation deck kind is more to be seriously affected normal production, manages, sale, is caused seriously to breeder, peasant
The problems such as economic loss, provides a kind of breeding method of white glume barley variety, cultivates and screening and identification goes out to adapt in the Yellow River and Huai He River
Area growth, white glume barley variety.
Technical solution
The present invention provides a kind of breeding method of white glume barley variety, comprising the following steps:
Make male parent with salt wheat No. 3, Supi 3 makees maternal, progress sexual hybridization, sexual hybridization F1Spend at training and EMS
The M that deposit number is CGMCC No.12660 is obtained after reason2The barley M20080805 of platinite glume, is carried out self propagated,
Selection: plant type is compact, plant height 67-80cm, and the time of infertility 208-213 days, base portion Trunk diameter 0.25-0.35cm, section was grown under fringe
13.5-15.0cm, spike length 6.0-7.0cm, heading to glume before maturation and auricle is white, is reflected by planting in selective nursery
It is fixed, obtain M3The white glume fine individual plant in generation.
Gained is stated into M3The fine individual plant in generation is selfed again, 2 row areas/strain, line-spacing 30cm, spacing in the rows 2cm, in selective nursery into
Row selects M4For yield with to compare Supi 3 close, economical character requires that plant height 67-80cm, plant type be compact, grain husk before heading to maturation
Shell and auricle are the white glume single plant of white, the effective fringe 52-62 of mu ten thousand.
The single plant of mass of 1000 kernel 36g or more is selected and remain after carrying out selection single plant in the selective nursery, then when species test indoors, directly
To M4In generation, has been bred as white glume barley variety.
A kind of breeding method of white glume barley variety, is bred as white glume New Barley Variety and names as salt
08036, which is: the kind semi-winterness, seedling half is upright, and for lowest order leaf leaf sheath without fine hair, leaf color is green, and heading is extremely
Glume and auricle are white before maturation, and the effective fringe 52-62 of mu ten thousand, heading stage is close with Supi 3, complete raw pain phase 207-
212 days；Fringe appearance is upright, and the wheat head is in two ribs, and fringe is tower-shaped, spike length 6.0-7.0cm, and Defined daily doses 24-30, mass of 1000 kernel 36-
40g。
Beneficial effect
The present invention handles the white glume barley variety salt 08036 of continuous multi-generation selfed breeding by Anther Culture and EMS,
The kind semi-winterness, seedling half is upright, and for lowest order leaf leaf sheath without fine hair, leaf color is green, and heading to glume before maturation and auricle is
White, boot leaf are hung down loosely, and stooling is stronger, and the percentage of earbearing tiller is high, and effective fringe 52-62 ten thousand of general mu, heading stage is close with Supi 3,
The complete raw 208-213 days pain phase；Awns is longer and spinosity, awns tip very slight color close grain husk and bloom, and fringe appearance is upright, and the wheat head is in two ribs, fringe
It is tower-shaped, spike length 6.0-7.0cm, Defined daily doses 24-30, mass of 1000 kernel 36-40g.
The white glume barley variety salt 08036 that the present invention cultivates has the following advantages that and good effect:
1, yield is high, stablizes, and yield potential is big
Participation this institute the new strain of barley qualification test of salt 08,036 2013~2014,478.4 kgs/acre of average product, than
Supi 3 low 1.2% is compareed, participates within 2014~2015 years the new strain of barley comparative test of this institute, 492.3 kilograms of average product/
Mu, than control Supi 3 volume increase 0.8%.The variety yield is substantially close with Supi 3, and yield level is relatively stable, demonstration examination
Test and experiment in cultivation the result shows that: the average yield per mu level of salt 08036 be 450~500 kilograms, yield potentiality is up to per mu yield 500
~530 kilograms.It is widely applied, domestic beer use and feeding notch pressure can not only be effectively relieved, while peasant can also be increased
Income creates social benefit.
2, varietY specificity is obvious
Salt 08036 is by making male parent with salt wheat No. 3, and Supi 3 makees maternal, progress sexual hybridization, in its F1In generation, carries out
Anther Culture and EMS processing selected and stablize, the special barley variety of cultivation by mostly generation.The appearance characteristics of the kind are significant,
Before showing as heading stage to maturation, glume and auricle are white, hence it is evident that are different from other barley varieties.This feature is the kind
Identity business card, the effect of anti-fake anti-vacation can be effectively played.
3, there is preferable ornamental value
Before earing to mature annesl, glume and auricle position show to be different from the kind due to the missing of chlorophyll
The white of other barley varieties, ornamental value are preferable.It can be used as a kind of ornamental plant, there is preferable ornamental value.
Four, biological deposits
White glume barley M20080805, classification naming is two rib barleys (Hordeum distichon), in 2016
It is preserved within August 22 days China Committee for Culture Collection of Microorganisms's common micro-organisms center, address Chaoyang District, Beijing City north year
The institute 3 of occasion West Road 1, institute of microbiology, the Chinese Academy of Sciences, deposit number are as follows: CGMCC No.12660.
Five, specific embodiment
The following is further explained with reference to the attached drawings white glume barley variety breeding method of the invention.
A) make male parent with salt wheat No. 3, Supi 3 makees maternal, progress sexual hybridization, acquisition F0For seed.
B) the F of above-mentioned acquisition0For seed, carry out planting to obtain F1For plant, F is taken1Monokaryon middle and advanced stage is in for pollen development
Tassel carries out Anther Culture, after the callus of Anther Culture is impregnated 3 hours in 0.4% EMS solution, sterile flushing,
It is inoculated in induced medium, differential medium culture is transferred to after 5 days.M is obtained after EMS is handled121 plants of Dai Lvmiao.
C) by the M of acquisition1The green seed of 21 plants of mutagenesis of generation plants Yu great Tian, selfing harvest M1For seed, by M1Fringe is planted for seed
Row, row long 2m, line-spacing 30cm, in Breeding Process, in M2Discovery heading to discovery glume and auricle during booting is white in generation
1 plant of the single plant of color, is marked, maturation harvest M2For single-strain seed.After harvest, by the single plant M of white2For Seed Deposit, preservation
Number are as follows: CGMCC No.12660.
D) by the white glume M of harvest2Self propagated is carried out for seed, the stable plant of selection traits is further identified.
Selection: white glume and auricle, plant type is compact, 67~80cm of plant height, and the time of infertility 208~213 days, base portion Trunk diameter
0.25-0.35cm saves long 13.5-15.0cm under fringe, ear to glume before maturation and auricle be white M3White glume is excellent
Good single plant.
E it) is then selected in selfing, 2 row areas/strain, line-spacing 30cm, spacing in the rows 2cm, from M3In generation, starts, and is choosing seeds by generation
Single plant after glume and auricle color, plant type, fringe color, leaf color Identification, Yield comparison, economical character and harvest is carried out in garden
The selection of shape.Yield and Supi 3 (control) are similar, and economical character requires that plant height 67-80cm, plant type be compact, the effective fringe 52 of mu
~62 ten thousand, heading to glume before maturation and auricle is white；Carry out artificial threshing after harvest again indoors again, when species test selects and remain
Grain plumpness is good；36 grams of mass of 1000 kernel or more of single plant；The fine individual plant that reservation meets breeding objective enters next breeding generation
In generation, carries out cultivation work.From M1In generation, starts, and has been bred as white glume barley variety salt 08036 after being selfed for 4 generations.
Subordinate list: the choosing of educating of present invention white glume barley variety salt 08036 is passed through
White 08036 cultural technigue of glume barley variety salt:
(1) elite seed: will carry out seed cleaning before sowing, guarantee 99% or more purity, 95% or more cleanliness, selected mistake
Strict control in journey, prevents mechanical admixture, and percentage of seedgermination must reach 85% or more.
(2) it arranges field, begin sowing in good time: in jiangsu coast Saline Region, before planting barley, it is necessary to it is whole to carry out field
Reason keeps field face smooth.After plantation, discharge conduit is excavated, which will want in 30~40cm or so, discharge conduit network
It is close, to guarantee that rainfall acts on the leaching salt salt discharge of soil, mitigate soil salt to the stress of barley growth.It is broadcast on salinization soil
Kind of time should October 23 between November 5, on desalination soil the sowing time select October 28 to November 15 for
Preferably.
(3) rational close planting: in Yancheng Littoral Zone, Jiangsu Province, under conditions of proper seeding, Basic Seedling is on salinization soil with 18
Ten thousand or so are advisable, and sowing date for early sowing or fertility level are higher, the lower field of soil salt content, and application rate can reduce by 10~
20%.Desalination soil field Basic Seedling is advisable with 160,000, and the visual concrete condition that sowing is slow or fertility is poor suitably increases Basic Seedling,
Guarantee has certain group.
(4) Optimum: according to the feature of Growth and Development of Barley rule and salt 08036, before the principle of fertilising should be
Promote, middle control, rear benefit, base manure may advantageously facilitate wheat seeding morning tiller enough, so that stem tiller number before the winter is reached 50~550,000, the control of highest stem tiller
1,100,000 hereinafter, suitably small amount supplements nitrogenous fertilizer after jointing, extend the functional period of blade, increases yield.In 400 kilograms of yield
Under level, optimum nitrogen rate is 25~30 purity nitrogens, and wherein base manure accounts for 70% or so, and top dressing accounts for 30%, in 2~3 ‰ salt marsh
Change on soil, 30kg/ mus of nitrogenous fertilizer, 20kg/ mus of phosphate fertilizer, on desalination soil, 35kg/ mus of nitrogenous fertilizer, 25kg/ mus of phosphate fertilizer be that optimum is big
The growth of wheat.
(5) it harvests: when salt 08036 has the bending of 30% tassel, can harvest, early receive influences its germination percentage in due course.And and
When threshing, ted and put in storage, can't stacking, influence the germination percentage and its seed color of wheat, guarantee seed hair with higher
Bud rate improves its commodity.
Claims (4)
1. a kind of breeding method of white glume barley variety, comprising:
The M for being CGMCC No.12660 with deposit number2Platinite glume barley M20080805, is carried out self propagated, selection:
Plant type is compact, plant height 67-80cm, the time of infertility 208-213 days, base portion Trunk diameter 0.25-0.35cm, saves long 13.5- under fringe
15.0cm, spike length 6.0-7.0cm, heading are white to glume before maturation and auricle, by the field plot test in selective nursery, are obtained
Obtain M3The fine individual plant of platinite glume.
2. a kind of breeding method of white glume barley variety according to claim 1, comprising:
By the M3The fine individual plant in generation is selfed again, and 2 row areas/strain, line-spacing 30cm, spacing in the rows 2cm are selected in selective nursery
M4For yield with to compare Supi 3 close, economical character require plant height 67-80cm, plant type compact, heading to glume before maturation and
Auricle is the white glume single plant of white, the effective fringe 52-62 of mu ten thousand.
3. a kind of breeding method of white glume barley variety according to claim 2, comprising:
The single plant that mass of 1000 kernel 36g or more is selected and remain after carrying out selection single plant in the selective nursery, then when species test indoors, until M4
In generation, has been bred as white glume barley variety.
4. a kind of breeding method of white glume barley variety according to claim 3, it is fixed to be bred as white glume barley variety
Entitled salt 08036, the varietal characteristic are:
The kind semi-winterness, seedling half is upright, and for lowest order leaf leaf sheath without fine hair, leaf color is green, heading to glume before maturation and auricle
It is white, the effective fringe 52-62 of mu ten thousand, heading stage is close with Supi 3, and the time of infertility 207-212 days；Fringe appearance is upright, wheat
Fringe is in two ribs, and fringe is tower-shaped, spike length 6.0-7.0cm, and Defined daily doses 24-30, mass of 1000 kernel 36-40g.
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| CN109997688A (en) * | 2019-05-10 | 2019-07-12 | 浙江省农业科学院 | The New Barley Variety method for integrate ornamental and feeding is cultivated in a kind of chemical mutagenesis |
| CN112369320A (en) * | 2020-12-02 | 2021-02-19 | 盐城师范学院 | Breeding method of multi-edge hybrid barley |
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Non-Patent Citations (2)
| Title |
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| "二棱大麦白化颖壳和旗叶紫耳突变的遗传分析";韩月澎等;《中国农业科学》;20031231;第36卷(第6期);第722-725页 * |
| "二棱大麦白化颖壳性状的遗传";张国荣等;《上海农业学报》;20001231;第16卷(第1期);第20-22页 * |
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