CN109729969A - The breeding method of the new monoid PLR germplasm of corn - Google Patents
The breeding method of the new monoid PLR germplasm of corn Download PDFInfo
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Abstract
The invention discloses a kind of breeding method of the new monoid PLR germplasm of corn, this method, which is selected in the production of temperate zone, shows good cenospecies, i.e. the hybridization F under Reid × PB groups and Reid × Lan. mode1‑F2For germplasm, Breeding of Inbred Lines and coordinate force is measured respectively；PLR monoid germplasm is set up again using coordinate force height and R/P the and R/L class self-mating system of economical character complementation.Using morning for strange land selection+S3-S4 for haploid breeding, according to said method, plant type, resistance, panicled characters etc. are strictly selected in florescence, harvest time, selects a collection of Elite inbred, ear barren tip is reduced, resistance, coordinate force, envelope point, seed-producing rate, dehydration property and adaptability are improved；The technical issues of cenospecies assembled can satisfy production requirement.
Description
Technical field
The present invention relates to crop breeding technical field, the breeding method of specially a kind of new monoid PLR germplasm of corn.
Background technique
Corn is the important cereal crops in the world, has important strategic position in grain security；It is simultaneously also processing
The important raw material such as industry.In China, these three types germplasm is main breeding germplasm, and main advantage and disadvantage are as follows:
P monoid (78599 class): this kind of main self-mating system of germplasm: 9195, P178, P138, neat 319 etc., plant type half it is compact and
Blade profile loosely is draped over one's shoulders, preferable resistance is shown to corn leaf spot evil, that is, it is good to hold green property.The most of plant of the cenospecies assembled are high
Big and dehydration is slower, and serious folding (lodging) and putrefactive phenomenon can be occurred by such as encountering strong wind or wet weather in stage of wax ripeness.
Lan. germplasm is the important corn germplasm of China Temperate Region in China, this kind of main self-mating system of germplasm: Mo17, wood 6,
PH4CV etc., plant type is compacter, and fruit ear is long, is dehydrated fast, seed-producing rate height, coordinate force height, moderate growth duration etc., but in China south
Leaf spot resistance is planted in square then strain and adaptability is poor.In China representative kind be in single No. 2, and Wumeng Shan Mountain area mainly with
Tuxpeno is assembled, and integrated adaptability is poor.
R monoid germplasm: for the Rui De (Reid) and Reid Improved lines applied in China's production.Representing self-mating system mainly has
9046, PH6WC, Zheng 58,7854 etc..This kind of self-mating system plant type is compact or half is compact, and High planting is preferable, and lodging resistance is stronger, comprehensive
Conjunction resistance, such as rust, banded sclerotial blight are poor.It mainly assembles, represents with Tropical germplsam in the cenospecies that Southwestern China regional group is matched
Cenospecies Nongda108, refined beautiful 889, Guidan No.8, Huaxing are No. 7 single etc., and the cenospecies assembled in this mode has their own advantages, from
Since these variety popularizations, a collection of cenospecies has been authorized in modification breeding, but does not all break through these kinds from general performance,
Also there is their limitation in terms of adaptability.
According to the demand of Southwestern China ecological environment and working condition, this team is done harm to using the disease-resistanting leaf spot of P groups of germplasm,
The Optimalities the such as Lan. fruit ear of germplasm is long, dehydration is fast, seed-producing rate is high, and the High planting of R monoid germplasm is preferable, lodging resistance is stronger
Shape, PLR germplasm is set up in fusion according to a certain percentage.By using morning for strange land selection+S3-S4For haploid breeding, breeding
A batch polymerization in the good characteristic for applying preferable three monoid self-mating systems in production, have coordinate force is high, dehydration is fast, disease resistance compared with
New self-mating system good, fruit ear envelope point is good, seed-producing rate is high, integrated adaptability is wide and High planting is good, greatly improves production and answers
With level, the strong advantage corn hybrid seed for adapting to Southwestern China mountain area to breeding has a very important role；Utilize these three types
Germplasm be fused into PLR germplasm carry out innovation Upgrading have not been reported.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of breeding methods of the new monoid PLR germplasm of corn, it is solved
The problems such as yielding ability of self-mating system itself, disease resistance, dehydration property, envelope point, intend overcome the deficiencies in the prior art.
In order to achieve the above object, the present invention is achieved by the following technical programs: the breeding of the new monoid PLR germplasm of corn
Method includes the following steps:
Step 1: choosing and show good cenospecies in the production of temperate zone, i.e., in Reid × Lan. and Reid × P groups of moulds
Hybridization F under formula1-F2For germplasm, difference Breeding of Inbred Lines, and measure coordinate force；Breeding goal is determined according to production requirement, is selected
R/P the and R/L class self-mating system of coordinate force height and economical character complementation out determines P, L, R monoid germplasm in the way of hybridizing backcrossing
Ratio, set up PLR monoid germplasm, become S0Generation；By S0For mixed pollination, mixed planting, mixing threshing, S is obtained1Generation；
Step 2: the S that step 1 is obtained1After seed plantation, individual plant selfing, by the method for step 1, stringent basis are carried out
Breeding goal selects disease resistance, resistance, tree characteristics and panicled characters, selects disease-resistant/inverse property and panicled characters are good
Single plant alternatively object, such bagging is from being sent to S2-3The fruit ear that its head progeny row stays is mixed threshing by generation respectively, is obtained
S3Or S4For seed；
Step 3: by the S in step 23-4Hybridize 200-300 fruit ear with induction system for seed plantation, is educated using monoploid
Kind method, selects 300-500 DH system；Then, Resistance Identification and agronomy are carried out to these DH systems under different ecological condition
Shape is selected and makees coordinate force test.
The germplasm of PLR monoid described in step 1 includes the self-mating system of P monoid, Lan. monoid and Reid monoid.
The panicled characters refer to that well envelope point is good, seed-producing rate is high, the inclined solids of seed and dehydration property are good.
Different ecological condition described in step 3, which refers to, represents Southwest Mountainous Areas planted in different ecological areas, i.e., High aititude ecotope, in
Height above sea level ecotope and low altitude area ecotope.
Beneficial effect
Compared with prior art, the present invention shows good cenospecies in the production of temperate zone by selecting, i.e., in Reid
Hybridization F under × Lan. and Reid × P groups of modes1-F2For germplasm, coordinate force is measured into Breeding of Inbred Lines respectively；Utilize coordinate force
High and economical character complementation R/P and R/L class self-mating system sets up PLR monoid germplasm again.Using morning for strange land selection+S3-S4Generation
Haploid breeding, there is the self-mating system selected height to match, in resist, wide suitable, dehydration is fast, high yield, seed-producing rate are high, the inclined solids of seed or
The features such as solids, compact anti-fall and plant type.It is mentioned without ear barren tip, seed-producing rate compared with QB512 using the self-mating system QB2896 that this method is bred as
High by 3.5%, leaf spot improves resistance 2-4 grades compared with QB1018；The combination assembled increases production 8.5%, 6.3% respectively, illustrates this method
Yield combining ability is effectively raised, while also improving dehydration property, seed-producing rate, disease resistance, planting density and adapting to mechanization
The production requirement of harvest etc..
Detailed description of the invention
Fig. 1 is flow chart of the invention.
Specific embodiment
The embodiment of the present invention: choosing and show good cenospecies in the production of temperate zone, i.e., in Reid × Lan. and
Hybridization F under Reid × P groups of modes1-F2For germplasm, difference Breeding of Inbred Lines, and measure coordinate force；It is determined according to production requirement
Breeding goal selects R/P the and R/L class self-mating system of coordinate force height and economical character complementation, by hybridize backcrossing in the way of determine P,
L, the ratio of R monoid germplasm sets up PLR monoid germplasm, becomes S0Generation；By S0For mixed pollination, mixed planting, threshing is mixed,
Obtain S1Generation；
Step 2: the S that step 1 is obtained1After seed plantation, individual plant selfing, by the method for step 1, stringent basis are carried out
Breeding goal selects disease resistance, resistance, tree characteristics and panicled characters, selects disease-resistant/inverse property and panicled characters are good
Single plant alternatively object, such bagging is from being sent to S2-3The fruit ear that its head progeny row stays is mixed threshing by generation respectively, is obtained
S3Or S4For seed；
Step 3: by the S in step 23-4Hybridize 200-300 fruit ear with induction system for seed plantation, is educated using monoploid
Kind method, selects 300-500 DH system；Then, Resistance Identification and agronomy are carried out to these DH systems under different ecological condition
Shape is selected and makees combining ability test examination.
The germplasm of PLR monoid described in step 1 includes the self-mating system of P monoid, Lan. monoid and Reid monoid.
The panicled characters refer to that well envelope point is good, seed-producing rate is high, the inclined solids of seed and dehydration property are good.
Different ecological condition described in step 3, which refers to, represents Southwest Mountainous Areas planted in different ecological areas (high, medium and low height above sea level).
It was verified that using preferable breeding hybridized self-mating system is applied in production, then pass through the advantage and disadvantage for pressing self-mating system
New monoid kind is set up, its advantage respectively is excavated, so that reaching disease-resistant, dehydration is fast, seed-producing rate height, wide adaptability, coordinate force
High and good plant type self-mating system is breeding objective；Therefore, it is preferable miscellaneous to be selected from performance in the production of Temperate Region in China for inventor seminar
Hand over kind of single 933 (R/P) of the Liao Dynasty and first beautiful serial (R/L), difference breeding at stabilization PR class self-mating system QB512, QB506 and LR class
Self-mating system QB1018, QB1013 etc., then PLR monoid germplasm is set up by certain ratio, by using morning for strange land selection+
S3-S4For haploid breeding means, be bred as a batch there is height to match, in resist, wide excellent selfing suitable, dehydration is fast, high yield and plant type are good
System, such as QB2894, QB2896, QB2908；The cenospecies assembled is participating in areas at different levels (pre-) Varieties Guizhou Province jade 966, north
More than 10, jade 1507 etc..
Be combined within 2014 identify and the economical character of self-mating system is recorded, the self-mating system of breeding name for
QB2896.Plant type is compact, and leaf color is dark green, and breeding time 110 days or so, 171 centimetres of plant height, 72 centimetres of Ear height, neat and consistent.Fringe
It is 16.1 centimetres long, thick 4.9 centimetres of fringe, 14 row of tassel row number, row grain number 36.6,31.6 grams of 100-grain weight, seed-producing rate 88%.Tassel
Primary branch 6, male flower sterile lemma, clever base do not develop the color, and anther does not develop the color；Female fringe filigree green.Seed yellow, Hard grain type, cob
White, fruit ear cartridge type, general combining ability are higher.The advantages of three (P, L, R) monoids of the selfing assembly, have height match,
In resist, the features such as wide suitable, dehydration is fast, high yield, seed-producing rate height, seed solids, compact anti-fall and plant type.
Plantation in 2014 is measured cross combination after self-mating system QB2896 is bred as, and selects miscellaneous excellent mode as PLR × Suwan and changes
Good system, QB2896 × XZ50612 identified that germplasm density is 4200 plants/acre in Guizhou, Yunnan multiple spot in 2015, examine after harvest
20.2 centimetres of seed pod spike length, thick 5.2 centimetres of fringe, all pilots do not have an ear barren tip, 16.2 row of tassel row number, and row grain number 36.6, hundred
Grain weighs 33.9 grams, which is half solids, and seed is big, deep, and seed-producing rate 88%, average yield per mu is up to 763.8 kilograms.Combine strain
It is 272.6 centimetres high, 82 centimetres of Ear height, drape over one's shoulders sparse under blade is slight, High planting is preferable.Disease-resistanting leaf spot reaches anti-above, the anti-fringe
Rotten and lodging.
Combination QB2896 × XZ50612, which names, participates in variety comparative test, yield and resistance for north 1507,2016-2017 of jade
It has outstanding performance；Participation association, Yunnan Province test in 2018 carries out machine acceptance test in Dongzhou Period in Chuxiong, and yield is compared up to 982 kilograms
Zhaohai standing grain 2 volume increase 31%；The breeding of the combination confirm a P groups, L groups, R monoid be fused into PLR new germ plasm monoid have height
Match, in resist, distinguishing features, this method such as wide suitable, dehydration is fast, high yield, seed-producing rate height, seed solids, anti-fall and plant type are compact
The self-mating system of cultivation, the cenospecies that assembles adapt to the ecological environment of Southwestern China mountain area complexity, improve dehydration property, seed-producing rate,
The production requirement of disease resistance, planting density and adaptation mechanized harvest etc..
By this method, a collection of excellent corn inbred line such as QB2894, QB2896, QB2908 etc. have finally been selected,
A collection of superior corn cross combination, which has been bred as, using them is participating in (pre-) the examination combination Guizhou Province jade 966 in areas at different levels, north jade 1507 etc.
10.It participates in the experiment and is mainly assembled with PLR × Suwan (or Improved lines) now, other group modes are in progress.
By utilizing the property such as PLR germplasm of the present invention and PR germplasm, LR kind confrontation ear barren tip length, seed-producing rate, water content, disease resistance
Shape compares, and QB2896 improves 3.5% compared with QB512 without ear barren tip, seed-producing rate, and leaf spot improves resistance 2-4 grades compared with QB1018.
1 PLR germplasm of table is compared with PR germplasm, LR germplasm objective trait
Three self-mating systems are identified after hybridizing with Testers QR273, are compared to combination yield, are bred as using this method
Self-mating system Q2896 compared with the combination that QR512, QB1018 are assembled, increase production 8.5%, 6.3% respectively, illustrate that this method effectively mentions
High yield combining ability.In addition, also increasing in terms of adaptability.
The Yield comparison of table 2 three self-mating systems and Testers QR273 cross combination
Above description has shown and described several preferred embodiments of invention, but as previously described, it should be understood that invention is not
It is confined to form disclosed herein, should not be regarded as an exclusion of other examples, and can be used for various other combinations, modification
And environment, and can be carried out within that scope of the inventive concept describe herein by the above teachings or related fields of technology or knowledge
Change.And changes and modifications made by those skilled in the art do not depart from the spirit and scope of invention, then it all should be in the appended power of invention
In the protection scope that benefit requires.
Claims (4)
1. a kind of breeding method of the new monoid PLR germplasm of corn, which comprises the steps of:
Step 1: choosing and show good cenospecies in the production of temperate zone, i.e., under Reid × Lan. and Reid × P groups of modes
Hybridization F1-F2For germplasm, difference Breeding of Inbred Lines, and measure coordinate force；Breeding goal is determined according to production requirement, is selected and is matched
The high R/P and R/L class self-mating system with economical character complementation of resultant force, determines the ratio of P, L, R monoid germplasm in the way of hybridizing backcrossing
Example sets up PLR monoid germplasm, becomes S0Generation；By S0For mixed planting, mixed pollination, mixing threshing, S is obtained1Generation；
Step 2: the S that step 1 is obtained1After seed plantation, individual plant selfing is carried out, by the method for step 1, strictly according to breeding
Target selects disease resistance, resistance, tree characteristics and panicled characters, selects disease-resistant/inverse property and the good list of panicled characters
Alternatively object, such bagging are sent to S certainly for strain2-3The fruit ear that its head progeny row stays is mixed threshing respectively, obtains S by generation3Or S4
For seed；
Step 3: by the S in step 23-4Hybridize 200-300 fruit ear with induction system for seed plantation, using haploid breeding side
Method selects 300-500 DH system；Then, under different ecological condition to these DH systems carry out Resistance Identification and economical character into
Row selects and makees coordinate force test.
2. the breeding method of the new monoid PLR germplasm of corn according to claim 1, it is characterised in that: described in step 1
PLR monoid germplasm includes the self-mating system of P monoid, Lan. monoid and Reid monoid.
3. the breeding method of the new monoid PLR germplasm of corn according to claim 1, it is characterised in that: the fringe portion
Shape refers to that well envelope point is good, seed-producing rate is high, the inclined solids of seed and dehydration property are good.
4. the breeding method of the new monoid PLR germplasm of corn according to claim 1, it is characterised in that: described in step 3
Different ecological condition, which refers to, represents Southwest Mountainous Areas planted in different ecological areas, i.e., High aititude ecotope, intermediate altitude ecotope and low altitude area are raw
State area.
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Cited By (5)
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| CN110679475A (en) * | 2019-11-12 | 2020-01-14 | 北京市农林科学院 | Innovative breeding method for yellow European group germplasm |
| CN110679476A (en) * | 2019-11-12 | 2020-01-14 | 北京市农林科学院 | Innovative breeding method of Huangrui group germplasm |
| CN111837942A (en) * | 2020-08-19 | 2020-10-30 | 江苏沿江地区农业科学研究所 | Valence-by-valence incremental germplasm innovation method for corn resistance yield multi-trait combined improvement |
| CN112514791A (en) * | 2020-12-18 | 2021-03-19 | 云南点谷农业科技有限公司 | Breeding method for breeding maize inbred line through high-medium-low altitude cyclic planting |
| CN113973709A (en) * | 2021-12-01 | 2022-01-28 | 安徽省农业科学院烟草研究所 | Method for creating high-yield multi-resistance maize inbred line suitable for 33-degree northern latitude areas |
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|---|---|---|---|---|
| CN110679475A (en) * | 2019-11-12 | 2020-01-14 | 北京市农林科学院 | Innovative breeding method for yellow European group germplasm |
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| CN111837942A (en) * | 2020-08-19 | 2020-10-30 | 江苏沿江地区农业科学研究所 | Valence-by-valence incremental germplasm innovation method for corn resistance yield multi-trait combined improvement |
| CN112514791A (en) * | 2020-12-18 | 2021-03-19 | 云南点谷农业科技有限公司 | Breeding method for breeding maize inbred line through high-medium-low altitude cyclic planting |
| CN113973709A (en) * | 2021-12-01 | 2022-01-28 | 安徽省农业科学院烟草研究所 | Method for creating high-yield multi-resistance maize inbred line suitable for 33-degree northern latitude areas |
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