CN105875398A - Method for breeding normal corn inbred line by using hard grain type waxy corn inbred line - Google Patents

Method for breeding normal corn inbred line by using hard grain type waxy corn inbred line Download PDF

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Publication number
CN105875398A
CN105875398A CN201610221344.4A CN201610221344A CN105875398A CN 105875398 A CN105875398 A CN 105875398A CN 201610221344 A CN201610221344 A CN 201610221344A CN 105875398 A CN105875398 A CN 105875398A
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corn
glutinous
solids
seed
fruit ear
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CN201610221344.4A
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CN105875398B (en
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刘宁
刘西美
石绪海
鲁亚萍
杨蒙
姬艳艳
张文超
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Shandong Zhongnong Tiantai Seeds Co Ltd
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Shandong Zhongnong Tiantai Seeds Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a method for breeding a normal corn inbred line by using a hard grain type waxy corn inbred line. According to the method, a corn inbred line is bred with a method of one-time hybridization of the early-maturing hard grain type waxy corn inbred line and repeated backcrossing of normal corn, so that rapid breeding of a new normal corn species with excellent quality, high unit weight and early maturity is realized. With the adoption of the method, the quality of the normal corn inbred line is improved, the hereditary basis of normal corn is widened, types of the normal corn are enriched, the corn growth period is shortened, and the method has the important social significance and economic value.

Description

A kind of method utilizing Hard grain type Waxy Corn Inbred Lines selection-breeding conventional corn selfing line
Technical field
The present invention relates to a kind of method utilizing Hard grain type Waxy Corn Inbred Lines improvement selection-breeding conventional corn selfing line, belong to agriculture Industry new variety selective breeding technology field.
Background technology
Semen Maydis is China's one of staple food crop, at present in state-owned more than 500,000,000 mu of Semen Maydiss, annual production accounts for total grain output 35%, it may be said that Maize Production yield is most important to grain-production, animal husbandry development and life of farmers.
China's Semen Maydis per unit area yield and total increase produced are largely dependent upon improving constantly of corn breeding level.In many increasings In product factor, the contribution rate of corn variety is to 40%.The heaviest based on Semen Maydis status in China's grain-production , the selection-breeding of corn variety is more and more important.
The corn variety great majority promoted in the market are Dens Equi type, half dent corn, and generally there is period of duration The features such as longer, unit weight is low, poor quality.The corn variety of period of duration length is planted in double-cropping system region, can have a strong impact on down Season crop implantation time and yield, affect farmers' income.
Glutinous Semen Maydis grain endosperm mostly is cutin, seed small volume, quality better.But Glutinous Semen Maydis filling process shows volume And for a long time shorter than conventional corn, moisture content of kernels day fall off rate than conventional corn, the feature, i.e. pustulation period such as fast be short, filling speed Hurry up, seed is dehydrated feature fast, that period of duration is short.
In sum along with quickening and the transformation of people's lives mode of Development of China's Urbanization, urgent needs are of fine quality, unit weight is high, ripe Phase Semen Maydis improved seeds early.And the present invention utilizes the feature that Hard grain type glutinous corn variety has precocity, quality is excellent, unit weight is high to change Good selection-breeding conventional corn, had both remained that conventional corn yield is high, disease resistance is good, the feature of wide adaptability, shortened again common jade The period of duration of rice, by the improvement of Dens Equi type for Hard grain type, improve the unit weight of kind, improve quality, application prospect is extensive.
The unit weight of general Flint corn is more than the unit weight of dent corn, and it is Semen Maydis matter that quality is better than the unit weight of corn kernel One of important indicator of amount and grade.Owing to unit weight is the index such as fixed of Semen Maydis, the most relevant with corn price, peasant in order to and Improving the income of oneself, the corn variety that unit weight will necessarily be selected higher is increased economic efficiency.In general unit weight with Vitreousness is ordered as bursting type more than Hard grain type more than Dens Equi type, the highest corn quality of unit weight in extremely notable positive correlation, general unit weight The best..
Summary of the invention
For solving the problem that above-mentioned prior art exists, it is an object of the invention to utilize precocity, Hard grain type Waxy Corn Inbred Lines to lead to Cross and once hybridize, repeatedly backcross with conventional corn, be bred as corn inbred line, excellent for selection-breeding quality, unit weight is high, corn inbred line A kind of method is provided, and then cultivates high-quality, precocity, conventional corn new varieties that unit weight is high, meet the market demand.
For reaching above-mentioned purpose, the technical scheme is that
(1) selecting conventional corn to be improved for female parent, Hard grain type Waxy Corn Inbred Lines is paternal hybrid, obtains hybridizing fruit ear.
(2) will be that male parent backcrosses with conventional corn selfing line after the seed plantation of hybridization fruit ear, obtain the fruit ear that backcrosses, single fringe takes off Grain, selects solids non-Glutinous Semen Maydis seed.
(3) backcross selecting and remain after the solids non-glutinous seed simple grain plantation of fruit ear, then be that male parent backcrosses with conventional corn selfing line, Obtain the fruit ear that backcrosses, single fringe threshing, select solids non-Glutinous Semen Maydis seed.
(4) by the non-Glutinous Semen Maydis of solids selected and remain by head progeny row seed, after simple grain plantation, a part of plant is used for backcrossing, and residue is planted Strain uses backbone system to be measured, and selects the corn ear that combining ability is the highest, period of duration is shorter, single fringe threshing, the non-glutinous jade of solids of selecting and remain Rice seed.
(5) repeat step (4), then carry out twice backcrossing and being measured, select the Semen Maydis that combining ability is the highest, period of duration is shorter Fruit ear, single fringe threshing, solids of selecting and remain non-Glutinous Semen Maydis seed.
(6) the non-glutinous seed of solids that will select, all individual plant bagging selfings after simple grain plantation, selection combining ability is the highest, fertility Phase is shorter, the non-glutinous fruit ear of solids, single fringe threshing.
(7) fruit ear will selected and remain in step (6), plants into head progeny row, whole individual plant bagging selfings, selects the non-glutinous fruit of solids Fringe, threshing can obtain Hard grain type conventional corn material.
Described conventional corn resource can be Dens Equi type or half dent corn selfing line.
Described Waxy Corn Inbred Lines can be Hard grain type Glutinous Semen Maydis corn hybrid seed or the Waxy Corn Inbred Lines of Hard grain type.
The present invention is to utilize Glutinous Semen Maydis solids, characteristic early of ripe phase to improve conventional corn resource, improves conventional corn product Matter, ripe phase and unit weight.
Compared with the breeding technique of existing conventional corn selfing line, the conventional corn selfing line using the present invention to select has Following advantage.
1, the quality of conventional corn selfing line is improved: the fine quality of Glutinous Semen Maydis is proceeded to conventional corn so that the common jade of cultivation Rice selfing line is nutritious/best in quality/and commodity is good, it is received by the market.
2, by the improvement of Dens Equi type conventional corn for Hard grain type conventional corn: the Hard grain type Glutinous Semen Maydis of selection is as backcrossing male parent so that The conventional corn selfing line cultivated, grain type is become Hard grain type from Dens Equi type, improves the unit weight of conventional corn.
3, the hereditary basis of conventional corn has been widened: the inventive method breaches the technical bottleneck that waxy corn landrace improvement is common, will The hereditary basis transformation of Glutinous Semen Maydis, in conventional corn, enriches the type of conventional corn, has established base for cultivating precocious conventional corn Plinth.
4, conventional corn period of duration is shortened: characteristic short for Glutinous Semen Maydis period of duration is fully proceeded in conventional corn by the present invention so that choosing The conventional corn reduction in the life period educated.
Detailed description of the invention:
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
" utilize Hard grain type Waxy Corn Inbred Lines that conventional corn inbred line breeding becomes Hard grain type, early ripening maize selfing line " specifically to walk Rapid:
The first step: the 5-9 month in 2006, is maternal at Linyi TT789, and sweet glutinous No. 3 is paternal hybrid 5-8 strain, obtains allocarpy Fringe, single fringe threshing.
Second step: in March ,-2007 in October, 2006, is that male parent backcrosses with TT789 after hybridization fruit ear is planted by Hainan In 5-8 strain generation, obtains the fruit ear that backcrosses, single fringe threshing, solids of selecting and remain non-Glutinous Semen Maydis seed.
3rd step: the 5-9 month in 2007, plants 50 strains in Hainan by the seed selected and remain, and is that male parent backcrosses with TT789, To the fruit ear that backcrosses, single fringe threshing, select solids non-Glutinous Semen Maydis seed.
4th step: in March ,-2008 in October, 2007, the seed will selected and remain in Linyi, by fruit ear, plant into head progeny row 30 Strain, wherein 15 strains are used for selfing;Other 15 strains utilize the mensuration that existing corn bone responsibilities carry out combining ability, and combining ability of selecting and remain is High, the period of duration milpa of 80-85 days, after single fringe threshing, solids of selecting and remain non-Glutinous Semen Maydis seed.
5th step: in March ,-2009 in October, 2008, in March ,-2010 in October, 2009, in Hainan, Linyi carries out Backcross and be measured for twice, select the corn ear that combining ability is the highest, period of duration is shorter, after single fringe threshing, the non-glutinous jade of solids of selecting and remain Rice seed.
6th step: in May, 2010 in JIUYUE ,-2010, the non-glutinous seed of solids that will select and remain, by fruit ear, plant into head progeny row 100 Strain, all individual plant bagging selfings after simple grain plantation, selection combining ability is the highest, the non-glutinous fruit ear of period of duration 80-85 days, solids, single Fringe threshing.
7th step: in March ,-2012 in October, 2011, the fruit ear will selected and remain in step (6), plants into head progeny row 50 strain, All individual plant bagging selfings, selection combining ability is the highest, the non-glutinous fruit ear of period of duration 80-85 days, solids, single fringe threshing, and threshing is i.e. Hard grain type conventional corn material TT201203056 can be obtained.
Embodiment 2
" utilize Hard grain type crossbreeded glutiuous maize that conventional corn selfing line (Dens Equi) selection-breeding becomes Hard grain type, early ripening maize selfing line " Concrete steps:
The first step: the 5-9 month in 2007, is maternal at Linyi Zheng Dan 958, and safe glutinous No. 3 of Waxy Corn Inbred Lines is paternal hybrid 5-8 Strain, obtains hybridizing fruit ear, single fringe threshing.
Second step: in March ,-2008 in October, 2007, returns for male parent with Zheng Dan 958 after hybridization fruit ear is planted by Hainan 5-8 strain generation is handed over to obtain the fruit ear that backcrosses, single fringe threshing, solids of selecting and remain non-Glutinous Semen Maydis seed.
3rd step: the 5-9 month in 2008, plants 50 strains in Hainan by the seed selected and remain, and backcrosses for male parent with Zheng Dan 958, Obtain the fruit ear that backcrosses, single fringe threshing, select solids non-Glutinous Semen Maydis seed.
4th step: in March ,-2009 in October, 2008, the seed will selected and remain in Linyi, by fruit ear, plant into head progeny row 30 Strain, wherein 15 strains are used for selfing;Other 15 strains utilize the mensuration that existing corn bone responsibilities carry out combining ability, and combining ability of selecting and remain is High, the period of duration milpa of 80-85 days, after single fringe threshing, solids of selecting and remain non-Glutinous Semen Maydis seed.
5th step: in March ,-2010 in October, 2009, in March ,-2011 in October, 2010, in Hainan, Linyi carries out Backcross and be measured for twice, select the corn ear that combining ability is the highest, period of duration is shorter, after single fringe threshing, the non-glutinous jade of solids of selecting and remain Rice seed.
6th step: in May, 2011 in JIUYUE ,-2011, the non-glutinous seed of solids that will select and remain, by fruit ear, plant into head progeny row 100 Strain, all individual plant bagging selfings after simple grain plantation, selection combining ability is the highest, the non-glutinous fruit ear of period of duration 80-85 days, solids, single Fringe threshing.
7th step: in March ,-2012 in October, 2011, the fruit ear will selected and remain in step (6), plants into head progeny row 50 strain, All individual plant bagging selfings, selection combining ability is the highest, the non-glutinous fruit ear of period of duration 80-85 days, solids, single fringe threshing, and threshing is i.e. Hard grain type conventional corn material TT201203086 can be obtained.
Embodiment 3
Conventional corn breeding of new variety method:
The first step: the 5-9 month in 2012, plants conventional corn selfing line TT201203056, TT201203086 each 250 in Linyi Strain, the 80 part conventional corn selfing linies different from source configure 160 parts Maize hybridized combinations, each cross combination hybridization 3 Fringe, the independent threshing of each cross combination is also numbered.
Second step: in March ,-2013 in October, 2012 in Hainan, in May, 2013 in JIUYUE ,-2013 in Linyi, Ji South, Heze, Jining, enter the seed of 180 cross combinations in zones of different in Hainan in March ,-2014 in October, 2013 Row anti-disease enzyme, annidation and Yield test, identified comparison, TTZH2014089, TTZH2014121, TTZH2014332 tri-combination performance is excellent, and principal character characteristic is as follows:
TTZH2014089 seedling sheath lilac, leaf color is dark green, and growing way is stronger.Plant type is compact, period of duration 101 days, plant height 277 lis Rice, Ear height 99 centimetres, Herb 18-20 sheet leaf, filigree lilac, flower pesticide lilac.Fruit ear cylinder type, spike length 17 centimetres, Thick 5 centimetres of fringe, average 16.8 row of tassel row number.Seed yellow, Hard grain type, cob is red.
TTZH2014121: summer sowing plant height 290 cm, fringe position 110 cm, plant type is compact, total leaf number About 19, about 6, leaf on fringe, spike length 19.5 centimetres, thick 5.0 centimetres of fringe, thick 3.0 centimetres of axle, tassel row number 16 is left The right side, fecundity is good, fruit ear cylindricality, and cob is red, Hard grain type, and grain yellow skin is resistance to close, disease-resistant, anti-fall.
TTZH2014332: seedling sheath lilac, leaf color is dark green, and growing way is stronger.Plant type is compact, plant height 257 centimetres, fringe position High 90 centimetres, Herb 18-20 sheet leaf, filigree lilac, flower pesticide lilac.Fruit ear cylinder type, spike length 18.2 centimetres, fringe Thick 5 centimetres, average 18 row of tassel row number.Seed yellow, Hard grain type, cob white.
The corn inbred line period of duration of above-mentioned institute selection-breeding about 83 days, plant type half is compact, plant height 168cm, Ear height 81cm, seedling sheath lilac, strain stem and leaf is green, filigree purple, the white axle of fruit ear, seed yellow, Hard grain type.Spike length 15 centimetres, thick 5 centimetres of fringe, tassel row number 16 row.
Period of duration about 83 days, plant type half is compact, plant height 173cm, Ear height 80cm, seedling sheath lilac, strain Stem and leaf light green, filigree is green, and flower pesticide is green, the red axle of fruit ear, seed yellow, Hard grain type.Spike length 13cm, thick 5 lis of fringe Rice, tassel row number 16-18 row.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is not limited thereto, and any The change expected without creative work work or replacement, all should contain within protection scope of the present invention.Therefore, originally The protection domain that the protection domain claim of invention is limited is as the criterion.

Claims (3)

1. the method utilizing Hard grain type Waxy Corn Inbred Lines selection-breeding conventional corn selfing line, it is characterised in that comprise the following steps:
(1) selecting conventional corn to be improved for female parent, Hard grain type Waxy Corn Inbred Lines is paternal hybrid, obtains hybridizing fruit ear;
(2) will be that male parent backcrosses with conventional corn selfing line after the seed plantation of hybridization fruit ear, obtain the fruit ear that backcrosses, single fringe threshing, select Solids non-Glutinous Semen Maydis seed;
(3) backcross selecting and remain after the solids non-glutinous seed simple grain plantation of fruit ear, then be that male parent backcrosses with conventional corn selfing line, obtain Backcross fruit ear, single fringe threshing, selects solids non-Glutinous Semen Maydis seed;
(4) by the non-Glutinous Semen Maydis of solids selected and remain by head progeny row seed, after simple grain plantation, a part of plant is used for backcrossing, and residue is adopted by plant It is measured with backbone system, selects the corn ear that combining ability is the highest, period of duration is shorter, single fringe threshing, solids of selecting and remain non-Glutinous Semen Maydis seed Grain;
(5) repeat step (4), then carry out twice backcrossing and being measured, select the corn ear that combining ability is the highest, period of duration is shorter, single Fringe threshing, solids of selecting and remain non-Glutinous Semen Maydis seed;
(6) the non-glutinous seed of solids that will select, all individual plant bagging selfings after simple grain plantation, select that combining ability is the highest, period of duration relatively The non-glutinous fruit ear of short, solids, single fringe threshing;
(7) fruit ear will selected and remain in step (6), plants into head progeny row, whole individual plant bagging selfings, selects the non-glutinous fruit ear of solids, threshing Hard grain type conventional corn material can be obtained.
Method the most according to claim 1, it is characterised in that: step 1) described in conventional corn resource to be improved can With Dens Equi type or the conventional corn selfing line of half Dens Equi type or cenospecies.
Method the most according to claim 1, it is characterised in that: step 1) described in Waxy Corn Inbred Lines can be Hard grain type Waxy Corn Inbred Lines or sweet glutinous maize self-bred line.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106171965A (en) * 2016-08-25 2016-12-07 通化市农业科学研究院 A kind of super many tassel row numbers Maize DH Lines leads to the selection of 15D969
CN111357645A (en) * 2020-05-12 2020-07-03 山东中农天泰种业有限公司 Method for breeding inbred line of dwarf corn
CN113615570A (en) * 2021-09-03 2021-11-09 山东中农天泰种业有限公司 Method for breeding maize inbred line resistant to ear rot

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CN1367999A (en) * 2001-02-06 2002-09-11 刘绍东 Method for breeding hybridized corn

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106171965A (en) * 2016-08-25 2016-12-07 通化市农业科学研究院 A kind of super many tassel row numbers Maize DH Lines leads to the selection of 15D969
CN111357645A (en) * 2020-05-12 2020-07-03 山东中农天泰种业有限公司 Method for breeding inbred line of dwarf corn
CN113615570A (en) * 2021-09-03 2021-11-09 山东中农天泰种业有限公司 Method for breeding maize inbred line resistant to ear rot

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Denomination of invention: A method for breeding common maize inbred lines by using hard kernel waxy maize inbred lines

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