CN106171965A - A kind of super many tassel row numbers Maize DH Lines leads to the selection of 15D969 - Google Patents
A kind of super many tassel row numbers Maize DH Lines leads to the selection of 15D969 Download PDFInfo
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- CN106171965A CN106171965A CN201610726826.5A CN201610726826A CN106171965A CN 106171965 A CN106171965 A CN 106171965A CN 201610726826 A CN201610726826 A CN 201610726826A CN 106171965 A CN106171965 A CN 106171965A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention belongs to corn variety breeding technique field, it is specifically related to the selection that a kind of super many tassel row numbers Maize DH Lines leads to 15D969, mainly include assembling the cultivation seed of single cross with Semen Maydis A6 and Semen Maydis PHB1M, the induction seed of single cross obtains monoploid, plantation double obtain Maize DH Lines, through screen described Maize DH Lines leads to the several step of 15D969.The Maize DH Lines that the selection provided by the present invention is obtained leads to 15D969, and tassel row number is stable at 30 row, far beyond 14～18 row tassel row numbers of ordinary maize kind, accordingly, has had Semen Maydis per mu yield and has been substantially improved.Meanwhile, the Maize DH Lines that the present invention selects leads to 15D969 also with good Comprehensive Traits, includes but not limited to lodging resistance, eurytopicity, yielding ability etc..
Description
Technical field
The invention belongs to corn variety breeding technique field, be specifically related to a kind of super many tassel row numbers Maize DH Lines and lead to 15D969
Selection.
Background technology
Tassel row number is the key factor determining corn yield, and at present, China produces the Corn Single-Cross Stock fringe of upper popularization and application
Line number majority is 16-18 row.
Forward position about corncob line number is studied, and presently preferred progress still can only achieve 20-22 row, and is widely popularized
The corn variety of 16-18 tassel row number does not has substantial breakthrough, even if being the corn variety of 20-22 tassel row number, the freshest
See.
To sum up, how by suitable selection-breeding means, cultivate the corn variety having essence breakthrough development on tassel row number,
So that corn yield is greatly improved, it it is corn breeding field problem demanding prompt solution.
Summary of the invention
In order to solve the problems referred to above that prior art exists, the invention provides a kind of super many line numbers Maize DH Lines and lead to
The selection of 15D969.The Maize DH Lines finally obtained by the selection of the present invention leads to 15D969, and tassel row number is up to 30
OK, it is significantly larger than the 16-18 tassel row number corn variety promoted at present, and compares to the 20-22 head progeny row of forefront, this area mutually
Number corn variety, it may have the breakthrough of essence, and then significantly improve the yield of Semen Maydis.Breakthrough development except tassel row number
Outward, the logical 15D969 of the DH system that the inventive method obtains also has every good Comprehensive Traits concurrently, in the side such as lodging resistance, eurytopicity
Also there is excellent behavior in face.
Corn Single-Cross Stock is assembled by maternal selfing line × male parent selfing line, and parent's tassel row number determines the seed of single cross
Tassel row number.The technology design of the present invention is i.e. to utilize haploid breeding technology, material based on A6 × PHB1M, through inducing,
Hainan doubles, locally selects, and finishing screen is selected the purpose Maize DH Lines of super many tassel row numbers and led to 15D969 kind.
At present, corn in Northeast China commercial variety (seed of single cross) is logical educates 99 (Tong Huayuan brings out) and the beautifulst 696 (pioneer's public affairs
Department) all there is bigger popularizing area.Logical educating 99 and be combined as PH6WC × A6, the beautifulst 696 are combined as PH6WC × PHB1M.Aforementioned
Technology design and on the basis of extensively the analyzing of the character of existing corn variety, the present invention establishes and utilizes A6 × PHB1M
Based on material, double to select tassel row number more than male parent DH system less than PHB1M of PHB1M, plant height and fringe position by induction
Basic research thinking.This thinking is by the recycling to original favored pattern, thus on the basis of tassel row number makes a breakthrough, with
Time maintain the logical lodging resistance educating 99 and the yielding ability of the beautifulst 696 and eurytopicity.
The technical solution adopted in the present invention particularly as follows:
A kind of super many tassel row numbers Maize DH Lines leads to the selection of 15D969, comprise the steps: S1, using Semen Maydis A6 as
Female parent, Semen Maydis PHB1M, as male parent, assembles cultivation and obtains seed of single cross seed of single cross F1 seed；S2, seed of single cross F1 seed is trained
Educate and induce and obtain monoploid seed；S3, monoploid seed is carried out plantation double cultivate, selfing i.e. obtains Maize DH Lines kind
Son；S4, plant and cultivate Maize DH Lines seed, lead to 15D969 through selecting described Maize DH Lines.
Stable in order to filter out character further, lodging tolerance is strong, yielding ability is strong, the super many tassel row numbers of eurytopicity
Maize DH Lines leads to 15D969, can be carried out the selection of the present invention further preferably by method parameter in detail below.
Semen Maydis A6 selection-breeding by the following method in step S1 and go out: with U.S. cenospecies as female parent, with red 340 miscellaneous for male parent
Hand over, obtain cenospecies；The continuous selfing of cenospecies, obtains described Semen Maydis A6.Described Semen Maydis A6 feature is: tassel row number 20-24 row, nothing point
Tiller, plant height less than 2m, emerge to loose powder number of days be 65 days and tassel branch 8.
The feature of Semen Maydis PHB1M described in step S1 is: has tiller, plant height 220cm～240cm, tassel row number 14-18 row, go out
Seedling to loose powder number of days is 65 days and tassel branch 12.
S2 induce haploid method particularly includes: plantation seed of single cross F1 seed, the long 5m of row, spacing in the rows 25, row spacing 60；
The second phase plantation of induction system point, first phase induction system and the seed of single cross F1 seed plantation same period, second phase induction system treats seed of single cross F1 seed
Plantation during grain Seed sprouting；When the female fringe of the F1 plant that seed of single cross F1 seed is grown up grows 3, overlap paper bag；When F1 plant is taken out
After silk 5d, utilizing the pollen mixture of the plant of induction system to pollinate the female fringe of F1 plant, the female fringe of each F1 plant all pollinates 2
Secondary；After pollination 3d, pluck paper bag；Lower fringe, selects top cover and is black and the colourless seed of seed embryo is monoploid seed, dry.
Described induction system lures SM6278-2 for Ji.
S3 doubles method particularly includes: in Hainan with row spacing 70 and spacing in the rows 15, monoploid seed is sowed, jointing
Obtaining haplobiont after phase roguing, after the flower pesticide maturation of haplobiont, the mature anther that cuts with a knife out is pollinated, and continues training
Educate and gather in the crops to obtain Maize DH Lines seed.To the pollination time of mature anther all after every morning 10.
In S4, plantation is cultivated method particularly includes: plant cultivating the Maize DH Lines seed obtained in Hainan after the accelerating germination of Jilin
Plant, after Jilin is screened, select described Maize DH Lines lead to 15D969.
In S4 screen specific standards be: field test: plant type is compact, Ye Shangju, plant height 145cm～250cm, fringe position 55cm
～150cm, spike length 12.2cm～19cm；Indoor species test standard: fringe is full without infected seed, long 0cm～5cm of the bald point of fringe, grain.
The invention have the benefit that
The concrete selection provided based on the above present invention, it is possible to quick, stable acquisition one tassel row number is stablized
The corn variety of 30 row.Genetic principle therein is: the seed of single cross F1 seed that material obtains based on A6 × PHB1M is to lose
Passing and separate the maximum generation, the monoploid grain that it induces is the haplotype (a set of chromosome) of the separated restructuring of gene, through nature
Each strain can the seed that is successfully obtained of selfing after doubling, it is simply that DH system seed, and this DH system seed is 100% to isozygoty.Through this locality
Select after qualification, become the logical 15D969 of DH system.The control gene of the tassel row number 30 of the logical 15D969 of DH system, is non-additive effect dystopy
Gene becomes the allele that can express after doubling, and is the present invention gene newly excavated of selection that passes through creativeness
Type.The appearance of this new gene type, extreme enrichment corn gene storehouse, have a good application prospect.
The present invention further studies confirmation, owing to logical 15D969 blood relationship is NSS (male parent system), with Reid female parent system group
Joining, the seed of single cross will have significant effect of increasing production (tassel row number increase), significant.Say from germplasm innovation angle, make Semen Maydis head progeny row
Number, far beyond traditional 16-18 tassel row number, reaches a new milestone.
Another that the present invention relates to studies confirmation further, will be the most excellent so that PH6WC × logical 15D969 assembles
The seed of single cross, on the basis of keeping tassel row number significantly to increase, other Comprehensive Traits every all show excellence.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of selection provided by the present invention.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme and corresponding effect are further explained, so that this
Skilled person can understand the present invention more fully.
In the present invention, as illustrated without other, term used is all generic term in industry, used method and apparatus
Be all industry universal method and apparatus, cited in the corn variety addressed be all industry in common commercially available prod.The present invention implements
The induction system used in example is that Ji lures SM6278-2, and buying is from academy of agricultural sciences of Jilin Province.
Embodiment 1:
The present embodiment 1 specifically provides the selection that a kind of super many tassel row numbers Maize DH Lines leads to 15D969, specific as follows:
The first step: in July, 2013, with Semen Maydis A6 as female parent, is father with Semen Maydis PHB1M and other corn varieties respectively
This, more than 10 maternal male parent combinations, assemble each 20kg of each combination respectively altogether.More than 10 maternal male parents are combined in the winter in 2013
In community, Sanya, Hainan Nan Bin farm, plantation is cultivated respectively, identifies the comprehensive resistance of these more than 10 maternal male parents combinations, finally with
A6 × PHB1M puts up the best performance, and tassel row number is 22 row, is assembled by A6 × PHB1M and cultivates the seed of single cross F1 seed that obtains and be applied to down
Step is cultivated.
A6 and PHB1M can be by commercially available purchase, it is also possible to cultivates by the following method and selects to obtain:
The selection-breeding of A6: with U.S. cenospecies as female parent, with red 340 as paternal hybrid, obtain cenospecies in 1998；Cenospecies is even
Continuous selfing, educates through Nan Fanbei, selected in 2002 and obtain described Semen Maydis A6.The feature of the A6 selected: tassel row number 20-24 row,
Without tiller, plant height less than 2m, emerge to loose powder number of days be 65 days and tassel branch 8.
The feature of selected PHB1M: have tiller, plant height 220cm～240cm, tassel row number 14-18 row, emerge to loose powder
Number of days is 65 days and tassel branch 12.
Second step: seed of single cross F1 seed is carried out great Qu cultivation and induces monoploid.2014 in the shore agriculture of Sanya, Hainan south
Seed of single cross F1 seed is planted with the long 5m of row, spacing in the rows 25, row spacing 60 kinds in field.Induction system lures SM6278-2 for Ji, point second phase plantation,
First phase induction system and the seed of single cross F1 seed plantation same period, second phase induction system plants when seed of single cross F1 seed Seed sprouting.
When the female fringe of the F1 plant that seed of single cross F1 seed is grown up grows 3, overlap paper bag.Induction is not bagging, when F1 plant is reeled off raw silk from cocoons 5d
After, utilize the pollen mixture of the plant of induction system that the female fringe of F1 plant is pollinated, the female fringe of each F1 plant is all pollinated 2 times；Award
After powder 3d, pluck paper bag.In mid-September, 2014 starts lower fringe, selects top cover and is black and the colourless seed of seed embryo is single times
Body seed, dries, and lower fringe continued on October 10th, 2014, amounts to and obtains monoploid seed totally 5238.
3rd step: acquisition monoploid seed is carried out cultivating plantation and induction doubles.On October 20th, 2014 is in Hainan three
Monoploid seed, with row spacing 70 and spacing in the rows 15, is sowed by farm, Ya Nan shore, obtains 2516 strain monoploid and plant after jointing stage roguing
Strain.After the flower pesticide maturation of haplobiont, the mature anther that cuts with a knife out is pollinated, and all exists the pollination time of mature anther
After every morning 10.Monoploid selfed seed 77 is gathered in the crops in the monoploid selfing that in mid-February, 2015 doubles for tassel altogether
Fringe, grain number per spike is 1～4, and adding multiplying power is 3.1%, collects monoploid selfed seed seed and i.e. obtains Maize DH Lines seed.
The tassel plant ratio that can double under field conditions (factors) is the highest, and mature anther is the most several, due to monoploid seed
Nutrition own is insufficient, and germination percentage is relatively low, high due to its temperature in Sanya, Hainan base, can quickly emerge under precision management；
Simultaneously as Sanya is tropical climate's feature, compares to the north mutually and can provide more favourable light for monoploid tassel Natural double
Temperature condition, is beneficial to improve the success rate of Natural double.
4th step: local plantation also selects super many tassel row numbers Semen Maydis.After accelerating germination, described Semen Maydis within 2015, is planted in Jilin
DH system seed 77 row, and the Maize DH Lines planted is carried out local screening, the specific standards of screening is: field test: plant type
Compact, Ye Shangju, plant height 145cm～250cm, fringe position 55cm～150cm, spike length 12.2cm～19cm；Indoor species test standard: fringe
Full without infected seed, long 0cm～5cm of the bald point of fringe, grain.26 parts of standard compliant DH systems are filtered out altogether based on this aforementioned screening criteria,
Selection percentage is 33.8%, and the design parameter index of these 26 parts of DH systems is listed in the table below 1.Selecting tassel row number has heredity prominent more than 30 row
The DH system of broken property progress is super many tassel row numbers Maize DH Lines and leads to 15D969, accounts for 1.3%, runic underline part in table 1
969 batch 2015DH systems be the logical 15D969 of the DH system that filters out.The fundamental characteristics of the logical 15D969 of DH system is: tassel row number is
30, resistant to lodging, disease-resistant, plant height and fringe position is relatively low, plant type is compact, emerging to loose powder number of days is 62d, tassel branch 12, blooms
Early (more Zao 3d than parents), pollen amount foot, loose powder time length, mid-early maturity.
Before select the logical 15D969 of DH system according to tassel row number, local screening also has following preferred screening criteria: field reflects
Fixed: select to emerge, plant type is compact, Ye Shangju, leaf are the narrowest, plant height, fringe position is low, tassel branch is many, pollen amount foot, the loose powder time
The diseases such as long, resistant to lodging, anti-tikka；Indoor qualification: fruit ear without infected seed, the bald point of fringe is short and small, grain is deep, grain is full.
Table 1
Embodiment 2:
The present embodiment 2 provides a kind of Maize DH Lines 15D969 selection-breeding extending further on the basis of embodiment 1 and improving
Method, during particularly as follows: the seed that super many tassel row numbers Maize DH Lines embodiment 1 obtained leads to 15D969 is planted, according to
Lower layout is planted: planting according to the mode of " wide narrow row intercycle " during plantation, the line-spacing of wide row is 95cm, narrow
Row line-spacing be 65cm, i.e. according to line-spacing " 95cm, 95cm, 65cm ", " 95cm, 95cm, 65cm ", " 95cm, 95cm,
65cm " .... form cycle alternation plantation.Two row Semen Maydiss of narrow row both sides are thin planting row, the adjacent jade in every thin planting row
The cave of rice kind plant hole is away from for 35cm, according to the mode maize planting of " four strain two ", described " four strain two " in every thin planting row
Two strain Semen Maydiss are planted, other maize seed in this thin planting row for the next maize seed plant hole at interval of four maize seed plant holes
Plant hole the most only plants a strain Semen Maydis.The thin planting row that a two row Semen Maydiss wherein behavior of wide row both sides shares with narrow row, another behavior
Dense planting row, according to the mode maize planting of " two strain three " in dense planting every day row, described " two strain three " are at interval of two jade
The next maize seed plant hole of rice kind plant hole plants three strain Semen Maydiss, and other maize seed plant hole in this dense planting row the most only plants two
Strain Semen Maydis.
Traditional corn planting layout type is to be equally spaced, so the most laborsaving during plantation, but does not but have
Field is made full use of, causes per mu yield the highest.Equidistantly the mode of plantation is planted in order to improving yield can select to increase
Density, can cause line space narrower so that field management is the most inconvenient after adding planting density.Prior art also has width
The mode of ridge interlaced arrangement, but it is low to yet suffer from land utilization ratio, the defect that yield is the highest.
The present embodiment 2, by the layout to field planting density and mode, has ensured making full use of of soil, field, simultaneously
There is the wide ridge row intersected, it is simple to field management.Meanwhile, prior, the layout type corn field of the present embodiment 2 ventilates suitable
Freely, illumination and land resources utilization are abundant, enabling obtain higher Semen Maydis per mu yield.In concrete example, it is directed to
Super many tassel row numbers Maize DH Lines that the embodiment of the present invention 1 provides leads to 15D969 and carries out contrast test, uses the field of the present embodiment 2
Layout planting patterns compares to tradition planting patterns and has increased production about 5.8%, and it is (under equal conditions, traditional that per mu yield reaches 698.2kg
Field layout planting patterns is 660kg).
Test example 1:
The present embodiment 1 Maize DH Lines 15D969 to obtaining in embodiment 1 carries out plantation and cultivates in Jilin, and observes
Itself and other corn variety character pair ratios.
Within 2015, it is respectively provided with Maize DH Lines in Jilin and leads to 15D969 selfing experimental plot, Semen Maydis A6 selfing experimental plot, jade
Rice PHB1M selfing experimental plot, A6 × PHB1M single cross experimental plot.Each experimental plot is all planted 4 row, row long 5m, spacing in the rows 25cm,
Row spacing 60, and keep the field management in 4 selfing experimental plots and control condition to keep consistent.After plantation cultivation terminates, sampling
All sample three times in interline continuous sampling 10 strain of each experimental plot, each experimental plot, and take the average of three times during comparison.
Result of the test is as follows:
Maize DH Lines leads to 15D969 selfing experimental plot: per mu yield is 660kg, and logical phene is: without tiller, average plant height
180 centimetres, 80 centimetres, average fringe position, emerge to loose powder number of days be 62d, average tassel branch number 12, average spike length 13 centimetres,
Average tassel row number 30 row, average 100-grain weight 28.8g, grain is deep.
A6 experimental plot: per mu yield is 418kg, logical phene is: without tiller, plant height 185, fringe position 72, emerge to
Loose powder number of days is 65d, tassel branch 8, spike length 15, tassel row number 20-24 row, 100-grain weight 27g.
PHB1M experimental plot: per mu yield is 589kg, logical phene is: has tiller, plant height 230, fringe position 90, emerges
It is 65d to loose powder number of days, tassel branch 12, spike length 16, tassel row number 14-18 row, 100-grain weight 37g.
A6 × PHB1M single cross experimental plot: per mu yield is 1034kg, logical phene is: tiller is many, mid-early maturity, emerge to
Loose powder number of days is 61d, plant height 330, fringe position 135, spike length 20, tassel row number 22,100-grain weight 41g.
By above contrast, the Maize DH Lines that selection of the present invention obtains leads to 15D969, has in terms of per mu yield
Pole is obviously improved (tassel row number pole significantly improves), and plant height decreases, and lodging tolerance is higher.Selection selection-breeding of the present invention
The Maize DH Lines gone out leads to 15D969 selfing can promote 71kg～242kg, growth ratio compared to existing corn variety per mu yield
12%～57.8%, and this is only in terms of selfing production.After assembling production according to the hybridization of industry corn variety or single cross
The industry experience deduction that yield is double, the logical 15D969 of the DH system of the present invention produces in popularization, with other corn variety groups concrete
Join single cross or hybridization produces, higher volume increase income can be obtained.
Test example 2:
This test example 2 specifically provides the lodging tolerance examination of the Maize DH Lines 15D969 that selection of the present invention obtains
Testing, method is as follows:
Within 2015, it is respectively provided with Maize DH Lines in Jilin and leads to 15D969 selfing experimental plot, Semen Maydis A6 selfing experimental plot, jade
Rice PHB1M selfing experimental plot, logical educate 99 (PH6WC × A6) single cross experimental plot, the beautifulst 696 (PH6WC × PHB1M) single cross test
Field.Each experimental plot is all planted 4 row, row long 5m, spacing in the rows 25cm, row spacing 60, and keeps the field in 4 selfing experimental plots to manage
Reason and control condition keep consistent.Record comparison each experimental plot late growth stage situation resistant to lodging.
Result of the test is as follows:
15D969 selfing experimental plot is led in DH system: upright, does not has tilt phenomenon.
A6 selfing experimental plot: major part is upright, a small amount of small angle inclination.
PHB1M selfing experimental plot: about 30% occurs.
Logical 99 (PH6WC × A6) single cross experimental plot of educating: major part is upright, a small amount of small angle inclination.
The beautifulst 696 (PH6WC × PHB1M) single cross experimental plot: about 30% occurs.
Interpretation of result: the logical 15D969 of DH system inherits and present the excellent lodging resistance of A6.
The present invention is not limited to above-mentioned preferred forms, and anyone can show that under the enlightenment of the present invention other are various
The product of form, no matter but in its details, make any change, every have same as the present application or akin technical scheme,
Within all falling within protection scope of the present invention.
Claims (10)
1. super many tassel row numbers Maize DH Lines leads to the selection of 15D969, it is characterised in that comprise the steps:
S1, using Semen Maydis A6 as female parent, Semen Maydis PHB1M, as male parent, assembles cultivation and obtains seed of single cross F1 seed；
S2, carry out cultivating and inducing obtaining monoploid seed by seed of single cross F1 seed；
S3, monoploid seed is carried out plantation double cultivate, selfing i.e. obtains Maize DH Lines seed；
S4, plant and cultivate Maize DH Lines seed, lead to 15D969 through selecting described Maize DH Lines.
Selection the most according to claim 1, it is characterised in that: Semen Maydis A6 selection-breeding by the following method in step S1 and
Go out: with U.S. cenospecies as female parent, with red 340 as paternal hybrid, obtain cenospecies；The continuous selfing of cenospecies, obtains described Semen Maydis
A6。
Selection the most according to claim 2, it is characterised in that: described Semen Maydis A6 feature is: tassel row number 20-24 row,
Without tiller, plant height less than 2m, emerge to loose powder number of days be 65 days and tassel branch 8.
Selection the most according to claim 1, it is characterised in that: the feature of Semen Maydis PHB1M described in step S1 is: have point
Tiller, plant height 220cm～240cm, tassel row number 14-18 row, emerge to loose powder number of days be 65 days and tassel branch 12.
Selection the most according to claim 1, it is characterised in that: S2 induce haploid method particularly includes: plantation
Seed of single cross F1 seed, the long 5m of row, spacing in the rows 25, row spacing 60；The second phase plantation of induction system point, first phase induction system and seed of single cross F1
The seed plantation same period, second phase induction system plants when seed of single cross F1 seed Seed sprouting；Treat the F1 that seed of single cross F1 seed is grown up
When the female fringe of plant grows 3, overlap paper bag；After F1 plant reels off raw silk from cocoons 5d, utilize the pollen mixture of the plant of induction system that F1 is planted
The female fringe pollination of strain, the female fringe of each F1 plant is all pollinated 2 times；After pollination 3d, pluck paper bag；Lower fringe, select top cover be black and
The colourless seed of seed embryo is monoploid seed, dries.
Selection the most according to claim 5, it is characterised in that: described induction system lures SM6278-2 for Ji.
Selection the most according to claim 1, it is characterised in that: S3 doubles method particularly includes: at Hainan Yi Long
Away from 70 and spacing in the rows 15, monoploid seed is sowed, after jointing stage roguing haplobiont, the flower pesticide of haplobiont becomes
After ripe, cut mature anther and pollinate, continue cultivation and gather in the crops to obtain Maize DH Lines seed.
Selection the most according to claim 7, it is characterised in that: to the pollination time of mature anther all in every morning
After 10.
Selection the most according to claim 8, it is characterised in that: in S4, plantation is cultivated method particularly includes: will be in sea
South is cultivated the Maize DH Lines seed obtained and is planted after the accelerating germination of Jilin, selects described Maize DH Lines and lead to after Jilin is screened
15D969。
Selection the most according to claim 9, it is characterised in that: screening specific standards in S4 is: field test: strain
Type is compact, Ye Shangju, plant height 145cm～250cm, fringe position 55cm～150cm, spike length 12.2cm～19cm；Indoor species test standard:
Fringe is full without infected seed, long 0cm～5cm of the bald point of fringe, grain.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106718834A (en) * | 2016-12-09 | 2017-05-31 | 通化市农业科学研究院 | A kind of selection of the logical D127 9 of corn Ear weight high-combining ability DH systems |
| WO2020112479A1 (en) * | 2018-11-27 | 2020-06-04 | The United States Of America, As Represented By The Secretary Of Agriculture | Production of haploid lolium |
| CN116724882A (en) * | 2023-07-07 | 2023-09-12 | 云南省农业科学院粮食作物研究所 | Method for cultivating multi-spike small waxy corn with 8-10 spike rows |
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| US11154022B2 (en) | 2018-11-27 | 2021-10-26 | The United States Of America, As Represented By The Secretary Of Agriculture | Production of haploid lolium |
| CN116724882A (en) * | 2023-07-07 | 2023-09-12 | 云南省农业科学院粮食作物研究所 | Method for cultivating multi-spike small waxy corn with 8-10 spike rows |
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| CN106171965B (en) | 2018-08-17 |
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