CN103798128B - A kind of method utilizing inbred line of sweet corn seed selection homotype Waxy Corn Inbred Lines - Google Patents
A kind of method utilizing inbred line of sweet corn seed selection homotype Waxy Corn Inbred Lines Download PDFInfo
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- CN103798128B CN103798128B CN201410046556.4A CN201410046556A CN103798128B CN 103798128 B CN103798128 B CN 103798128B CN 201410046556 A CN201410046556 A CN 201410046556A CN 103798128 B CN103798128 B CN 103798128B
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Abstract
The present invention relates to a kind of method utilizing inbred line of sweet corn seed selection homotype Waxy Corn Inbred Lines, comprise the steps: 1) select waxy corn landrace to be paternal hybrid for maternal, inbred line of sweet corn, obtain hybridizing fruit ear; 2) seed of hybridization fruit ear being planted rear inbred line of sweet corn is that male parent backcrosses, and obtains the fruit ear that backcrosses; 3) will backcross fruit ear threshing non-sweet seed of selecting and remain, all individual plant bagging selfings after simple grain plantation, select the fruit ear occurring glutinous matter seed, single fringe threshing, the non-sweet seed of glutinous matter of selecting and remain; 4) the non-sweet seed plantation of the glutinous matter of step 3) being selected and remain, repeats step 1) to step 3) three samsaras, obtains sweet corn from the waxy corn selfing material being homotype; 5), after selfing material simple grain plantation step 4) obtained, the selfing of individual plant bagging, selects not occur that the fruit ear threshing of sweet grain can obtain the Waxy Corn Inbred Lines of inbred line of sweet corn homotype.
Description
Technical field
The present invention relates to a kind of method utilizing inbred line of sweet corn seed selection homotype Waxy Corn Inbred Lines, belong to agriculture new variety selective breeding technology field.
Background technology
Inbred line breeding is the important step of agriculture rearing new variety, and Waxy Maize is no exception, and the cultivation of excellent Waxy Maize new varieties be unable to do without the seed selection of Elite inbred.In recent years, along with the raising of living standards of the people and the reinforcement of health care consciousness, people propose more and more higher requirement to the taste quality of Waxy Maize product, but the resource that existing glutinous corn breeding resource is relatively deficient, best in quality, resistance is good, output is outstanding especially lacks.Breeder is finding the New methods in working of waxy corn germplasm innovation always.Although waxy corn and sweet corn belong to fresh edible maize kind, controlled by different gene locis, become non-glutinous non-sweet corn after phase mutual cross, be ordinary maize, lose its original edibility.For this reason, waxy corn and Sweet Corn Germplasm resource be good modified utilization each other never, how the characteristic of sweet corn resource fine quality is improved the important subject that waxy corn landrace has become numerous breeders, sweet corn and waxy corn landrace are organically combined, the quality of improvement waxy corn landrace, is bred as the inbred line that product are of fine quality, resistance, coordinate force are high, cultivates excellent New Waxy Maize Hybrids, meet the new demand of market to Quality type waxy corn product, there are wide market prospects undoubtedly.
Summary of the invention
For solving above-mentioned prior art Problems existing, the object of the invention is to utilize inbred line of sweet corn to be bred as homotype Waxy Corn Inbred Lines by backcross transformation, for seed selection product are of fine quality, the Waxy Corn Inbred Lines of better resistance provides a kind of method, and then cultivate the good New Waxy Maize Hybrids of high-quality, high resistance, commodity, meet the need of market.
For achieving the above object, technical scheme of the present invention is:
Utilize a method for inbred line of sweet corn seed selection homotype Waxy Corn Inbred Lines, comprise the steps:
1) select waxy corn landrace to be paternal hybrid for maternal, inbred line of sweet corn, obtain hybridizing fruit ear;
2) seed of hybridization fruit ear being planted rear inbred line of sweet corn is that male parent backcrosses, and obtains the fruit ear that backcrosses;
3) will backcross fruit ear threshing non-sweet seed of selecting and remain, all individual plant bagging selfings after simple grain plantation, select the fruit ear occurring glutinous matter seed, single fringe threshing, the non-sweet seed of glutinous matter of selecting and remain;
4) the non-sweet seed plantation of the glutinous matter of step 3) being selected and remain, repeats step 1) to step 3) three samsaras, obtains the waxy corn selfing material of inbred line of sweet corn homotype;
5), after selfing material simple grain plantation step 4) obtained, the selfing of individual plant bagging, selects not occur that the fruit ear threshing of sweet grain can obtain the Waxy Corn Inbred Lines of inbred line of sweet corn homotype.
Further, above-mentioned steps 1) described in waxy corn landrace can be waxy corn Hybrid, waxy corn landrace and Waxy Corn Inbred Lines.
Further, above-mentioned steps 1) described in inbred line of sweet corn can be general sweet, strengthen sweet and super-sweet corn inbred line.
The present invention utilizes the colory characteristic of sweet corn to improve waxy corn landrace, improve the quality of waxy corn, enriched the hereditary basis of waxy corn, enhance resistance, seed selection in Breeding Process, transfer method have convenient selection and operability in addition, for cultivation high-quality, high resistance Waxy Corn Inbred Lines provide new channel, and then seed selection waxy corn elite hybrid, meet the need of market.The method has not yet to see report.
Compare with the breeding technique of existing Waxy Corn Inbred Lines, the Waxy Corn Inbred Lines tool adopting the present invention to select has the following advantages:
1, the quality of waxy corn is improved.The fine quality of sweet corn is proceeded to waxy corn, make to cultivate thinner, the sugariness of Waxy Corn Inbred Lines pericarp increases, glutinous middle band is sweet, mouthfeel is unique, the crossbreeded glutiuous maize of seed selection is nutritious, best in quality, commodity is good, is received by the market.
2, the hereditary basis of waxy corn has been widened.The inventive method breaches the technical bottleneck that Sweety and waxy corn resource can not improve each other, by the hereditary basis transformation of sweet corn in waxy corn, has enriched the type of waxy corn, so for cultivate strong stress resistance, wide adaptability crossbreeded glutiuous maize lay the foundation.
3, waxy corn Heterosis has been enriched.The inbred line of sweet corn pairing backcrossing transformation being in different Heterotic Groups is become homotype Waxy Corn Inbred Lines, directly can assembling cross-fertilize seed, ensure that the hybrid vigour of F1, the blindness avoiding waxy corn combination is measured, decrease breeding cost and labour, improve breeding efficiency.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1
The concrete steps " utilizing Sweet corn inbred line that crossbreeded glutiuous maize backcross breeding is become homotype Waxy Corn Inbred Lines ":
The first step: 3 ~ June in 2005 is at Nanjing use " Yunuo No.7 (state examines beautiful 2003069) " (genotype wxwxSu
1su
1) be maternal, Sweet corn inbred line " 984 " (genotype WxWxsu
1su
1) be paternal hybrid 2 ~ 3 strain, obtain hybridization fruit ear (genotype WxwxSu
1su
1), single fringe threshing;
Second step: 7 ~ October in 2005 to backcross 2 ~ 3 strains for male parent with " 984 " after hybridization fruit ear seed is planted by Nanjing, obtaining backcrossing, (seed genotype is WxSu to fruit ear
1su
1or Wxsu
1su
1), single fringe threshing, select and remain non-sweet seed (genotype WxSu
1su
1);
3rd step: the non-sweet seed simple grain that second step obtains in Hainan year February by November, 2005 ~ 2006 plants 100 strains, the whole bagging selfing of individual plant, selects the fruit ear occurring glutinous matter seed, single fringe threshing, non-sweet seed (the genotype wxwxSu of glutinous matter of selecting and remain
1);
4th step: the non-sweet seed of glutinous matter the 3rd step selected and remain in Nanjing in March, 2006 plants 20 strains, between in March, 2006 ~ 2009 year February, repeat the first step to three samsaras of the 3rd step in Nanjing or Hainan, obtain the waxy corn selfing material (984wxwxSu of " 984 " homotype
1);
5th step: 3 ~ June in 2009, the whole bagging selfing of individual plant, selected not occur that the fruit ear threshing of sweet grain can obtain the Waxy Corn Inbred Lines (984wxwxSu of " 984 " homotype after the selfing material simple grain that the 4th step obtains by Nanjing plants 50 strains
1su
1), expand numerous by selfing or the mixed method handed over.
The concrete steps of embodiment 2 " utilizing reinforcement inbred line of sweet corn that Waxy Corn Inbred Lines backcross breeding is become homotype Waxy Corn Inbred Lines ":
The first step: 3 ~ June in 2006 is at Nanjing Waxy Corn Inbred Lines " logical is 5 " (genotype wxwxSu
1su
1seSe) be maternal, strengthen inbred line of sweet corn " Mo17su
1su
1sese " (genotype WxWxsu
1su
1sese) be paternal hybrid 2 ~ 3 strain, obtain hybridization fruit ear (genotype WxwxSu
1su
1sese), single fringe threshing;
Second step: 7 ~ October in 2006 uses " Mo17su after hybridization fruit ear seed is planted by Nanjing
1su
1sese " for male parent backcrosses 2 ~ 3 strains, obtaining backcrossing, (seed genotype is WxSu to fruit ear
1su
1or Wxsu
1su
1), single fringe threshing, select and remain non-sweet seed (genotype WxSu
1su
1);
3rd step: the non-sweet seed simple grain that second step obtains in Hainan year February by November, 2006 ~ 2007 plants 100 strains, the whole bagging selfing of individual plant, selects the fruit ear occurring glutinous matter seed, single fringe threshing, non-sweet seed (the genotype wxwxSu of glutinous matter of selecting and remain
1);
4th step: the non-sweet seed of glutinous matter the 3rd step selected and remain in Nanjing in March, 2007 plants 20 strains, repeats the first step to three samsaras of the 3rd step in Nanjing or Hainan, obtains " Mo17su between in March, 2007 ~ 2010 year February
1su
1sese " the waxy corn selfing material (Mo17wxwxSu of homotype
1);
5th step: 3 ~ June in 2010, the whole bagging selfing of individual plant, selected not occur that the fruit ear threshing of sweet grain can obtain " Mo17su after the selfing material simple grain that the 4th step obtains by Nanjing plants 50 strains
1su
1sese " Waxy Corn Inbred Lines (Mo17wxwxSu of homotype
1su
1), expand numerous by selfing or the mixed method handed over.
Embodiment 3: the concrete steps of " utilizing super-sweet corn inbred line that waxy corn landrace backcross breeding is become homotype Waxy Corn Inbred Lines ":
The first step: 3 ~ June in 2006 is at Nanjing " four tunnels glutinous (Yunnan local kind) " (genotype wxwxSh
2sh
2) be maternal, super-sweet corn inbred line " 403-11 " (genotype WxWxsh
2sh
2) be paternal hybrid 2 ~ 3 strain, obtain hybridization fruit ear (genotype WxwxSh
2sh
2), single fringe threshing;
Second step: 7 ~ October in 2006 to backcross 2 ~ 3 strains for male parent with " 403-11 " after hybridization fruit ear seed is planted by Nanjing, obtaining backcrossing, (seed genotype is WxSh to fruit ear
2sh
2or Wxsh
2sh
2), single fringe threshing, select and remain non-sweet seed (genotype WxSh
2sh
2);
3rd step: the non-sweet seed simple grain that second step obtains in Hainan year February by November, 2006 ~ 2007 plants 100 strains, the whole bagging selfing of individual plant, selects the fruit ear occurring glutinous matter seed, single fringe threshing, non-sweet seed (the genotype wxwxSh of glutinous matter of selecting and remain
2);
4th step: the non-sweet seed of glutinous matter the 3rd step selected and remain in Nanjing in March, 2007 plants 20 strains, between in March, 2007 ~ 2010 year February, repeat the first step to three samsaras of the 3rd step in Nanjing or Hainan, obtain the waxy corn selfing material (403-11wxwxSh of " 403-11 " homotype
2);
5th step: 3 ~ June in 2010, the whole bagging selfing of individual plant, selected not occur that the fruit ear threshing of sweet grain can obtain the Waxy Corn Inbred Lines (403-11wxwxSh of " 403-11 " homotype after the selfing material simple grain that the 4th step obtains by Nanjing plants 50 strains
2sh
2), expand numerous by selfing or the mixed method handed over.
Embodiment 4 New Waxy Maize Hybrids selection:
The first step: 3 ~ June in 2012 is in each 100 strains of Nanjing plantation Waxy Corn Inbred Lines 984wxwx, Mo17wxwx and 403-11wxwx, 58 part (number: SN1 ~ SN58) Waxy Corn Inbred Lines different from source prepare 174 waxy corn hybrid combinations, each combined hybrid 3 fringe, mixing threshing is also numbered;
Second step: 7 ~ September in 2012 in Nanjing, in November, 2012 ~ 2013 year January in Hainan, the seed of 174 hybrid combinations carries out field plot test in Various Seasonal and ecotope in Nanjing by 3 ~ June in 2013, product are carried out than selecting to tree characteristics, output, quality, commodity and resistance etc., result shows: 984wxwx Χ SN-06, Mo17wxwx Χ SN-15, Mo17wxwx Χ SN-29,403-11wxwx Χ SN-34 tetra-combine general performance excellent, its principal character characteristic:
1) 984wxwx Χ SN-06: about 84 days, In Nanjing breeding time (emerging to fresh fringe of gathering), total number of sheets 18 ~ 19, plant type half is compact, plant height 199 centimetres, Ear height 85 centimetres, average spike weight 246 grams, the fresh fringe of per mu yield about 830 kilograms.Spike length 19.8 centimetres, thick 4.6 ~ 4.9 centimetres of fringe, tassel row number 14 ~ 16, several 46 of row grain, neat kernel arrangement, grain white, and uniformity, best in quality, resistance is better.
2) Mo17wxwx Χ SN-15: about 81 days breeding time of In Nanjing, total number of sheets 18, plant type is compact, plant height 189 centimetres, Ear height 72 centimetres, average single fringe fringe 234 grams, the fresh fringe of per mu yield about 800 kilograms.Spike length 19.6 centimetres, thick 4.7 centimetres of fringe, tassel row number 14 ~ 16, several 42 of row grain, fringe shape is attractive in appearance, seed is neat, waxy strong, product are of fine quality.
3) Mo17wxwx Χ SN-29: about 88 days breeding time of In Nanjing, total number of sheets 19 ~ 20, plant type half is compact, plant height 213 centimetres, Ear height 88 centimetres, average single fringe fringe 252 grams, the fresh fringe of per mu yield about 880 kilograms.Spike length 21 centimetres, thick 4.9 ~ 5.1 centimetres of fringe, tassel row number 14 ~ 16, several 48 of row grain, fruit ear marshalling, grain white, waxy strong, pericarp is thin, comprehensive resistance is excellent.
4) 403-11wxwx Χ SN-34: about 86 days breeding time of In Nanjing, total number of sheets 19 ~ 20, plant type half is compact, plant height 206 centimetres, Ear height 81 centimetres, average single fringe fringe 232 grams, the fresh fringe of per mu yield about 860 kilograms.Spike length 20.5 centimetres, thick 4.7 ~ 4.9 centimetres of fringe, tassel row number 12 ~ 16, several 42 of row grain, fruit ear arrangement closely, grain white, and bright color after boiling, waxy strong, pericarp is thin, the multiple diseases such as the Chinese People's Anti-Japanese Military and Political College, helminthosporium maydis, banded sclerotial blight, rust.
The above, be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any change of expecting without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (1)
1. utilize a method for inbred line of sweet corn seed selection homotype Waxy Corn Inbred Lines, it is characterized in that comprising the steps:
1) select waxy corn landrace to be paternal hybrid for maternal, inbred line of sweet corn, obtain hybridizing fruit ear;
2) seed of hybridization fruit ear being planted rear inbred line of sweet corn is that male parent backcrosses, and obtains the fruit ear that backcrosses;
3) will backcross fruit ear threshing non-sweet seed of selecting and remain, all individual plant bagging selfings after simple grain plantation, select the fruit ear occurring glutinous matter seed, single fringe threshing, the non-sweet seed of glutinous matter of selecting and remain;
4) by step 3) the non-sweet seed plantation of the glutinous matter of selecting and remain, repeat step 1) to step 3) three samsaras, obtain the waxy corn selfing material of inbred line of sweet corn homotype;
5) by step 4) after the plantation of the selfing material simple grain that obtains, the selfing of individual plant bagging, selects not occur that the fruit ear threshing of sweet grain can obtain the Waxy Corn Inbred Lines of inbred line of sweet corn homotype;
Described female parent is genotype wxwxSu
1su
1" Yunuo No.7, male parent is genotype WxWxsu
1su
1sweet corn inbred line " 984 ";
Or female parent is waxy corn genotype wxwxSu
1su
1seSe inbred line " logical is 5 ", male parent is genotype WxWxsu
1su
1sese strengthens inbred line of sweet corn " Mo17su
1su
1sese ";
Or female genotype wxwxSh
2sh
2" four tunnels glutinous (Yunnan local kind) ", male parent is genotype WxWxsh
2sh
2super-sweet corn inbred line " 403-11 ".
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| CN105961186A (en) * | 2016-05-11 | 2016-09-28 | 南京市蔬菜科学研究所 | Breeding method of excellent super sweet corn inbreeding line |
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| CN102217524B (en) * | 2011-05-11 | 2012-11-14 | 南京市蔬菜科学研究所 | Breeding method for sweet glutinous maize self-bred line |
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