CN102217524B

CN102217524B - Breeding method for sweet glutinous maize self-bred line

Info

Publication number: CN102217524B
Application number: CN201110120903XA
Authority: CN
Inventors: 戴惠学; 熊元忠; 陈舜权; 李英; 胡俏强
Original assignee: NANJING INSTITUTE OF VEGETABLE SCIENCE
Current assignee: NANJING INSTITUTE OF VEGETABLE SCIENCE
Priority date: 2011-05-11
Filing date: 2011-05-11
Publication date: 2012-11-14
Anticipated expiration: 2031-05-11
Also published as: CN102217524A

Abstract

The invention relates to a breeding method for a sweet glutinous maize self-bred line, which comprises the following steps of: 1) hybridizing Nannongziyunuo No.1 serving as purple glutinous maize and Jingtian No.3 serving as ultra-sweet maize to obtain hybridized ears; 2) planting purple seeds, and backcrossing by using the Nannongziyunuo No.1 as a male parent to obtain backcrossed ears; 3) planting purple glutinous seeds, performing bagging selfing, namely selecting and remaining the ears of ultra-sweet seeds, picking all purple glutinous seeds of the ears out, and performing single-grain sowing and single-plant bagging selfing; and 4) selecting and remaining the ears of the ultra-sweet seeds, picking white ultra-sweet seeds of the ears, sowing, performing 3 to 4 generations of continuous selfing by combining stress resistance and quality characters and selecting excellent individual plants to breed a stable sweet glutinous maize self-bred line TN-01. By the breeding method, the advantages of high quality, high stress resistance and high adaptability of the bred hybrid seeds are ensured, and the bred self-bred line can hybridize the glutinous maize and the ultra-sweet maize.

Description

A kind of selection of sweet-waxy maizes inbred line

Technical field

The present invention relates to a kind of selection of sweet-waxy maizes inbred line, belong to agriculture new variety selective breeding technology field.

Background technology

The seed selection of good sweet-waxy maizes inbred line is the basis of rearing new variety.Along with the raising of living standards of the people and the reinforcement of health care consciousness; Quality and commodity to sweet-waxy maizes have proposed new requirement; It is more and more welcome particularly to add sweet-waxy maizes (glutinous sweet seed is along with arranging on the fruit ear) and super-sweet corn; Area under cultivation enlarges year by year, but the relative vacancy of good kind, and it is particularly important to select therefore that the dulcification that can be mixed with high-quality is glutinous, the inbred line of super-sweet corn seems.The inbred line that at present the waxy corn material is obtained with conventional selection can not make and add sweet-waxy maizes, can not with super-sweet corn inbred line preparing hybrid kind, can not share the advantage between glutinous and sweet two mutation types.

Summary of the invention

The objective of the invention is provides a kind of method for the sweet-waxy maizes inbred line of seed selection high-quality, better resistance, so cultivate high-quality, better resistance, commodity good add sweet-waxy maizes and super-sweet corn new varieties, meet the need of market.

The present invention adopts following technical scheme:

1) selects brilliant sweet No. 3 hybridization of purple waxy corn Nannongziyunuo No.1 and super-sweet corn kind, obtain hybridizing fruit ear;

2) the purple seed that will hybridize on the fruit ear is chosen the back plantation, backcrosses as male parent with Nannongziyunuo No.1, obtains the fruit ear of backcrossing;

3) the glutinous matter seed plantation of the purple back bagging selfing on the fruit ear of selecting to backcross, selecting and remain the fruit ear of ultra sweet seed occurs, the glutinous matter seed of its all purples is selected and monoseeding the selfing of individual plant bagging;

Aforementioned ultra sweet seed with the naked eye can be distinguished, and is the seed of shrivelled shrinkage.

4) selecting and remain in the selfing fruit ear that obtains the fruit ear of ultra sweet seed occurs, and the ultra sweet seed of its white is chosen the back sowing, selects the continuous selfing 3-4 of fine individual plant generation in conjunction with resistance, quality trait, breeds stable sweet-waxy maizes inbred line TN-01.

Above-mentioned steps 1) and 2) the purple waxy corn Nannongziyunuo No.1 of described selection is brilliant sweet No. 3 of maternal, super-sweet corn

-1-

Be paternal hybrid, from the hybridization fruit ear, select purple seed plantation back to backcross as male parent with Nannongziyunuo No.1.

Above-mentioned steps 3) describedly on the fruit ear of backcrossing, select the glutinous matter seed plantation of purple back selfing, with the glutinous matter seed of all purples on the fruit ear that ultra sweet seed occurs select, monoseeding and bagging selfing;

Above-mentioned steps 4) fruit ear of ultra sweet seed appears in described selection, sows after choosing the ultra sweet seed of white, combines resistance, the continuous selfing 3-4 of quality trait selection fine individual plant generation, has guaranteed the stability and the resistance of selected sweet-waxy maizes inbred line.

Owing to selected the waxy corn of high-quality and super-sweet corn kind to come the seed selection inbred line, guaranteed the fine quality of selected inbred line, expanded hereditary basis, for the sweet-waxy maizes crossbreed of preparing high-quality, degeneration-resistant, eurytopicity, high-combining ability provides good parent.The inbred line TN-01 phenotype of institute's seed selection is the sweet glutinous dual-purpose type corn inbred line of ultra sugariness shape; Do not appear in the newspapers as yet at present; This inbred line TN-01 can obtain adding the sweet-waxy maizes new varieties with the waxy corn hybridization between selfed lines; The ratio of glutinous matter seed and saccharoid seed is about 3:1 on the fruit ear, has guaranteed glutinous property, has improved sugariness, has improved quality; Also can obtain the super-sweet corn new varieties of high-quality, good stress resistance, coordinate force height, wide adaptability with the super-sweet corn hybridization between selfed lines.

Compare with prior art, the sweet-waxy maizes inbred line TN-01 that adopts the present invention to select has following advantage:

1. obtain colour (purple white randomized arrangement) dulcification glutinous (glutinous matter seed and saccharoid seed ratio are about 3:1 on the fruit ear) corn variety with white waxy corn hybridization between selfed lines; This kind has not only guaranteed glutinous property, has increased the seed sugariness, and the outward appearance commodity is better.

2. (ratio of purple seed and white-kernel is about 3:1 to obtain colour with the hybridization between selfed lines of purple waxy corn; And the purple seed is that glutinous matter, white-kernel are ultra sweet type) dulcification glutinous (glutinous matter seed and saccharoid seed ratio are about 3:1 on the fruit ear) corn variety; Have glutinous during this kind is not only glutinous but also sweet, sweet; Purple seed ratio is about 3/4 on the fruit ear, has commodity preferably, has satisfied the market new demand.

3. obtain with the super-sweet corn hybridization between selfed lines that high-quality, better resistance, commodity are good, the super-sweet corn new varieties of wide adaptability.Obtain yellowish-white alternate kind, obtain pure white kind with yellow hybridization between selfed lines, can work as conventional super-sweet corn inbred line and use with white hybridization between selfed lines.

4. economical character is good.Inbred line tassel branch with the present invention breeds is flourishing, pollen amount is big, the pollen duration is longer, the suitable male parent of working as during hybrid seeding; Owing to combined the advantage of waxy corn and two mutation types of super-sweet corn, selected inbred line has wide adaptability, strong stress resistance and characteristics such as best in quality.

The feature of inbred line TN-01: this inbred line was sowed in spring 98 days, autumn sowing the time of infertility about 85 days in the area, Nanjing, the seedling leaf sheath is green, and the leaf look dark green, and cane is green, 182 centimetres of plant heights, and the fringe position is high 78 centimetres, total number of sheets 19～

-2-

20, the fringe upper blade is 5～6, and the tassel branch is flourishing, pollen amount is big, the green white of filigree, and the fruit ear cartridge type, spike length 15～

16 centimetres, tassel row number 14～16 row, several about 38 of row grain, grain white, thousand fringe 126 grams, the field performance:

Diseases such as the Chinese People's Anti-Japanese Military and Political College, helminthosporium maydis, head smut, banded sclerotial blight, rough dwarf disease.

Embodiment

Used Nannongziyunuo No.1 is commercially available getting with brilliant sweet No. 3 seeds among the present invention.

Use the concrete steps of the inventive method seed selection sweet-waxy maizes inbred line TN-01:

The first step: purple waxy corn Nannongziyunuo No.1 is hybridized for male parent for brilliant sweet No. 3 for maternal, super-sweet corn kind, obtain hybridizing fruit ear.

Second step: select the plantation of purple seed, backcross, obtain the fruit ear of backcrossing with Nannongziyunuo No.1.

The 3rd goes on foot: go on foot second and select the glutinous matter seed of purple on the fruit ear that obtains, and simple grain plantation and whole bagging selfings, the fruit ear of the ultra sweet seed of appearance of selecting and remain is selected the glutinous matter seed of its all purples and monoseeding the selfing of individual plant bagging.

The 4th step: with selecting the ultra sweet seed plantation of white on the 3rd fruit ear that obtain of step, combine quality, resistance and plant type to select 3～4 generations of continuous selfing, promptly breed sweet-waxy maizes inbred line TN-01, this inbred line can or be mixed friendship and breed original seed through selfing.

Claims

1. the selection of a sweet-waxy maizes inbred line is characterized in that comprising the steps:

1) selects purple waxy corn Nannongziyunuo No.1 to hybridize for male parent for brilliant sweet No. 3, obtain hybridizing fruit ear for maternal, super-sweet corn;

2) select purple seed plantation back to backcross as male parent, obtain the fruit ear of backcrossing with Nannongziyunuo No.1;

3) select the glutinous matter seed plantation of purple back bagging selfing, selecting and remain the fruit ear of ultra sweet seed occurs, the glutinous matter seed of its all purples is selected and monoseeding the selfing of individual plant bagging;

4) select and remain and the fruit ear of ultra sweet seed occurs, the ultra sweet seed of its white is chosen the back sowing, combine resistance, the continuous selfing 3-4 of quality trait selection fine individual plant generation, breed stable sweet-waxy maizes inbred line TN-01.

2. selection according to claim 1; It is characterized in that: the fruit ear of ultra sweet seed appears in the described selection of step 4); Sow after choosing the ultra sweet seed of white; Select 3～4 generations of the continuous selfing of fine individual plant in conjunction with resistance, quality trait, guaranteed the stability and the resistance of selected sweet-waxy maizes inbred line.