CN106508665A - Method for selecting seeds of high sweet-resistant waxy corn varieties - Google Patents

Method for selecting seeds of high sweet-resistant waxy corn varieties Download PDF

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CN106508665A
CN106508665A CN201610958615.4A CN201610958615A CN106508665A CN 106508665 A CN106508665 A CN 106508665A CN 201610958615 A CN201610958615 A CN 201610958615A CN 106508665 A CN106508665 A CN 106508665A
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seed
resistant
corn
plantation
waxy
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章清杞
陈幼玉
杨帆
李美德
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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Abstract

The invention discloses a method for selecting seeds of high sweet-resistant waxy corn varieties, which belongs to the technical field of corn disease-resistant breeding. The method takes high-yield high-quality sweet corn varieties, waxy corn varieties, high bipolaris maydis-resistant varieties, high sphacelotheca reiliana-resistant varieties, high ostrinia nubilalis-resistant varieties, and high puccinia polysora-resistant varieties as a parent body, through hybridization and double-cross polymerization, through waxy performance, sweet degree and disease and insect resistance of multi-generation seeds are identified and screened, and the sweet waxy corn varieties capable of resisting four insect diseases, having waxy performance, sweet degree, and excellent agronomic and output property can be obtained.

Description

A kind of selection of high anti-sweet-waxy maizes kind
Technical field
Present invention relates particularly to a kind of selection of high anti-sweet-waxy maizes kind, belongs to corn breading for pest resistance technology Field.
Background technology
Corn seed milk stage sugar content is high, rich in water-soluble polysaccharide, protein, various free in its milk stage seed Amino acid, vitamin etc., also referred to as fruit-vegetable type corn, are increasingly subject to consumers in general with its unique local flavor and nutritive value Favor, and those thin skins are also referred to as fruit corn without slag, the sweet crisp high-quality corn that can be eaten something rare of mouthfeel.Waxy corn is also referred to as wax Matter corn, is the new type of Jing artificially breedings of being undergone mutation again by conventional corn, its seed endosperm starch be 100% Chain starch, glues soft and is rich in waxy, be commonly called as glutinous corn after boiling.Due in waxy corn seed starch completely by amylopectin structure Into, and by waxy control so as to there is in edible quality and industrial production special purposes.Corn and waxy corn due to It is with higher nutritive value and special local flavor and mouthfeel, deep to be welcome by consumer, it is the higher corn product of economic benefit Type, planting this two classes corn at present has become one of effective way of China's agricultural volume increase and increasing peasant income.With economy Sustainable development and living standards of the people raising, while people are sharply increased to fresh edible maize demand, to fresh edible maize Quality it is also proposed higher requirement, be no longer satisfied with edible waxy corn and corn, and be intended to increase waxy corn Sugariness, sweet-waxy maizes have the local flavor of corn and waxy corn concurrently, very popular, and its market price is also higher.Due to The harm of Corn diseases and insect pests, annual production loss 10-15%, reaches 30-40% during big generation, serious plot even has no harvest, it has also become carry The limiting factor of high corn yield and quality.Corn Common Diseases have ten several, due to the popularization and application of corn hybrid seed, corn Helminthosporium maydis, maize head smut, corn borer, corn embroider the rapid prevalence of disease, become the Major Diseases in production, in recent years, these Pest and disease damage increases year by year in China's occurrence degree, it has also become cause the Major Diseases of the corn underproduction.The preventing and treating of maize diseases, to adopt It is with disease-resistant variety, especially simultaneous to resist how anti-kind to be most economical effective measures generally acknowledged in the world.However, due to China it is disease-resistant Sweet-waxy maizes germ plasm resource is few, and mostly monoclonal antibody kind, and disease-resistant, the wide disease-resistant sweet-waxy maizes kind of seed selection height is particularly significant.
The content of the invention
It is an object of the invention to provide a kind of selection of high anti-sweet-waxy maizes kind, so as to greatly improve sweet-waxy maizes product The resistance planted.
The present invention is with high yield and high quality Sweet corn variety, glutinous corn variety, high-resistance corn snout moth's larva kind, high-resistance corn head smut It is parent that kind, high-resistance corn helminthosporium maydis kind, high-resistance corn embroider sick kind, by the polymerization that hybridizes, reestablishes diplomatic relations, and the kind in Jing many generations Sub waxy, sugariness, Resistant evaluation and screening, being bred as can simultaneously high anti-4 kinds of pest and disease damages, high waxy good, sugariness, agronomy and yield The excellent high anti-sweet-waxy maizes kind of shape.The kind has excellent agronomy and yield traits, plants high anti-sweet-waxy maizes kind Environmental pollution can also be reduced while yield is increased.
To reach object above, the present invention is employed the following technical solutions：
1）With Sweet corn variety（P0）, glutinous corn variety（P1）, high-resistance corn head smut kind（P2）, high-resistance corn snout moth's larva kind （P3）, high-resistance corn helminthosporium maydis kind（P4）, high-resistance corn embroider sick kind（P5）For hybrid strain, make hybridization P0/P2, P0/P3, P0/P4, P0/P5, P1/P2, P1/P3, P1/P4, P1/P5, obtain 8 hybridization F1, respectively with F1-P02、F1-P03、F1-P04、 F1-P05 、F1-P12、F1-P13、F1-P14、F1- P15 is represented；
2）Plantation F1-P02、F1-P03、F1-P04、F1-P05 、F1-P12、F1-P13、F1-P14、F1- P15 is each 10 plants, florescence Bagging selfing, obtains F2For seed F2-P02、F2-P03、F2-P04、F2-P05 、F2-P12、F2-P13、F2-P14、F2-P15；
3）F is planted respectively2For seed, each combination plantation 40-50 strain, and Resistant identification is carried out, each combination only identifies which Corresponding resistant parent institute disease and insect resistance species, filters out the excellent individual plant 6-8 of high disease and insect resistance, leaf morphology and yield traits Bagging selfing, maturity period individual plant sowing, F2-P02、F2-P03、F2-P04、F2Seed on-P05 plant carries out sugariness identification, F2- P12、F2-P13、F2-P14、F2Seed on-P15 plant carries out waxy indentification, and performance sugariness proterties is selected in each combination respectively Or the seed of waxy proterties is each 20, obtains F3For seed F3-P02、F3-P03、F3-P04、F3-P05 、F3-P12、F3-P13、F3- P14、F3-P15；
4）Plantation F3For seed, florescence makees hybridization F3-P02/F3-P03、F3-P04/F3-P05、F3-P12/F3-P13、F3- P14/F3- P15, must hybridize F1, respectively with F1-P023、F1-P045 、F1-P123、F1- P145 is represented；
5）Plantation F1-P023、F1-P045 、F1-P123、F1- P145, bagging selfing obtain F2For seed F2-P023、F2-P045、 F2-P123、F2-P145；
6）Plantation F2-P023、F2-P045、F2-P123、F2- P145 is each 40 plants, and carries out Resistant identification, and each combination is only reflected Fixed its corresponding 2 resistant parents institute disease and insect resistance species, filters out the excellent list of high disease and insect resistance, leaf morphology and yield traits The each 5 bagging selfings of strain, maturity period individual plant sowing, F2-P023、F2Seed on-P045 plant carries out sugariness identification, F2- P123、F2Seed on-P145 plant carries out waxy indentification, and performance sugariness proterties or waxy proterties are selected in each combination respectively Seed is each 20, obtains F3For seed F3-P023、F3-P045、F3-P123、F3-P145；
7）Plantation parent F3-P023、F3-P045、F3-P123、F3- P145 seeds, florescence make hybridization F3-P023/F3-P045、 F3-P123/F3- P145, must reestablish diplomatic relations F1, with F1-02345、F1- 12345 represent；
8）Plantation F1-02345、F1- 12345, florescence makees hybridization F1-02345/F1- 12345, obtain F1, with F1- 012345 represents；
9）Plantation F1- 012345, bagging selfing obtains F2- 012345, plant F2- 012345 common 60-70 strains, and carry out Resistant mirror Fixed, the whole 4 resistant parent institute disease and insect resistance species of identification filter out high anti-4 kinds of pest and disease damages, leaf morphology and yield traitses excellent Different 8-10 bagging selfing of individual plant, maturity period individual plant sowing, seed carry out sugariness and waxy indentification, select while showing sugariness 20, the seed of proterties and waxy proterties, obtains F3For seed F3-012345；
10）Plantation F3For seed F3- 012345 totally 20 plants, and Resistant identification is carried out, all 4 resistant parents institutes are disease-resistant for identification Insect pest species, filters out high 5 bagging selfings of individual plant for resisting 4 kinds of pest and disease damages, leaf morphology and yield traitses excellent, maturity period list Strain sowing, obtains F4For seed F4-012345；
11）Strain plants F4For seed F4- 012345 totally 5 strains, each 30 plants of strain plantation, the maturity period carries out leaf morphology Identify with yield traits, screen 1 most excellent strain of leaf morphology and yield traits, therefrom select 2-3 elite seedling set Bag selfing, i.e. incubation leaf morphology, agronomy and yield traits are excellent, the high sweet glutinous maize self-bred line for resisting 4 kinds of pest and disease damages.
Sweet corn variety be Shandong it is sweet beautiful No. 1 or Shandong it is sweet beautiful No. 2, glutinous corn variety is that Shanghai is beautiful glutinous No. 1 or SW22（Ding Xiangzhen, Waxy corn SW70 and SW22 are the new starch synthase gene afunction multimutation bodies of a class, molecular cytobiology report, 2008,41（05））, high-resistance corn head smut kind is Mo17 or lucky 465（Sun Zhichao, Jilin Province maize head smut research Progress and disease-resistant variety seed selection, Maize Sciences, 2007,15 (2)）, high-resistance corn snout moth's larva kind be middle section 1, high-resistance corn stigma Sick kind is C103（Ma Chunhong, impact (bulletin) of the Bipolaris maydis race T different disposal to 103 blade of corn C, plant Protection scientific and technical innovation and developing china plant protection association Annual Conference collection of thesis in 2008,2008：318-320）, Gao Kangyu It is neat 319 that rice embroiders sick kind（Ye Jincai, neat 319 and Lu Dan 50 corns of high-resistance corn rust, agricultural knowledge, 2000（07））.
Beneficial effects of the present invention：
Effect of the invention is that respectively control corn borer, maize head smut, corn southern leaf blight, corn are embroidered the anti-source base of disease Because importing in high yield sweet-waxy maizes kind by hybridization, the polymerization of back cross breeding method, leaf morphology, agronomy and yield traits are bred as Excellent, the high sweet glutinous maize self-bred line for resisting 4 kinds of pest and disease damages, high anti-sweet-waxy maizes kind is because of the common sweet-waxy maizes product of disease-resistant per mu of ratio Planting increases yield 15-20%, and spraying pesticide cost 100-150 is first for reduction, and per mu can increase economic benefit 350-400 unit.
Specific embodiment
Embodiment 1：
1）It is sweet beautiful No. 1 with Sweet corn variety Shandong（P0）, glutinous corn variety Shanghai is beautiful glutinous No. 1（P1）, high-resistance corn head smut kind Mo17（P2）, section 1 in high-resistance corn snout moth's larva kind（P3）, high-resistance corn helminthosporium maydis kind C103（P4）, the sick product of high-resistance corn embroidery Plant neat 319（P5）For hybrid strain, make hybridization P0/P2, P0/P3, P0/P4, P0/P5, P1/P2, P1/P3, P1/P4, P1/ P5, obtains 8 hybridization F1, respectively with F1-P02、F1-P03、F1-P04、F1-P05 、F1-P12、F1-P13、F1-P14、F1- P15 tables Show；
2）Plantation F1-P02、F1-P03、F1-P04、F1-P05、F1-P12、F1-P13、F1-P14、F1- P15 is each 10 plants, florescence Bagging selfing, obtains F2For seed F2-P02、F2-P03、F2-P04、F2-P05 、F2-P12、F2-P13、F2-P14、F2-P15；
3）F is planted respectively2For seed, each 40 plants of combination plantation, and Resistant identification is carried out, each combination only identifies which is corresponding Resistant parent institute disease and insect resistance species, filters out excellent 6 baggings of individual plant of high disease and insect resistance, leaf morphology and yield traits certainly Hand over, maturity period individual plant sowing, F2-P02、F2-P03、F2-P04、F2Seed on-P05 plant carries out sugariness identification, F2-P12、 F2-P13、F2-P14、F2Seed on-P15 plant carries out waxy indentification, and performance sugariness proterties or glutinous is selected in each combination respectively The seed of property proterties is each 20, obtains F3For seed F3-P02、F3-P03、F3-P04、F3-P05 、F3-P12、F3-P13、F3-P14、 F3-P15；
4）Plantation F3For seed, florescence makees hybridization F3-P02/F3-P03、F3-P04/F3-P05、F3-P12/F3-P13、F3- P14/F3- P15, must hybridize F1, respectively with F1-P023、F1-P045 、F1-P123、F1- P145 is represented；
5）Plantation F1-P023、F1-P045、F1-P123、F1- P145, bagging selfing obtain F2For seed F2-P023、F2-P045、F2- P123、F2-P145；
6）Plantation F2-P023、F2-P045、F2-P123、F2- P145 is each 40 plants, and carries out Resistant identification, and each combination is only reflected Fixed its corresponding 2 resistant parents institute disease and insect resistance species, filters out the excellent list of high disease and insect resistance, leaf morphology and yield traits The each 5 bagging selfings of strain, maturity period individual plant sowing, F2-P023、F2Seed on-P045 plant carries out sugariness identification, F2- P123、F2Seed on-P145 plant carries out waxy indentification, and performance sugariness proterties or waxy proterties are selected in each combination respectively Seed is each 20, obtains F3For seed F3-P023、F3-P045、F3-P123、F3-P145；
7）Plantation parent F3-P023、F3-P045、F3-P123、F3- P145 seeds, florescence make hybridization F3-P023/F3-P045、 F3-P123/F3- P145, must reestablish diplomatic relations F1, with F1-02345、F1- 12345 represent；
8）Plantation F1-02345、F1- 12345, florescence makees hybridization F1-02345/F1- 12345, obtain F1, with F1- 012345 represents；
9）Plantation F1- 012345, bagging selfing obtains F2- 012345, plant F2- 012345 totally 60 plants, and Resistant identification is carried out, The whole 4 resistant parent institute disease and insect resistance species of identification, filter out high anti-4 kinds of pest and disease damages, leaf morphology and yield traitses excellent 8 bagging selfings of individual plant, maturity period individual plant sowing, seed carry out sugariness and waxy indentification, select while showing sugariness proterties With 20, the seed of waxy proterties, F is obtained3For seed F3-012345；
10）Plantation F3For seed F3- 012345 totally 20 plants, and Resistant identification is carried out, all 4 resistant parents institutes are disease-resistant for identification Insect pest species, filters out high 5 bagging selfings of individual plant for resisting 4 kinds of pest and disease damages, leaf morphology and yield traitses excellent, maturity period list Strain sowing, obtains F4For seed F4-012345；
11）Strain plants F4For seed F4- 012345 totally 5 strains, each 30 plants of strain plantation, the maturity period carries out leaf morphology Identify with yield traits, screen 1 most excellent strain of leaf morphology and yield traits, therefrom select 2 elite seedling baggings Selfing, i.e. incubation leaf morphology, agronomy and yield traits are excellent, the high sweet glutinous maize self-bred line for resisting 4 kinds of pest and disease damages.
High anti-sweet-waxy maizes kind increases yield 15% because of the common sweet-waxy maizes kind of disease-resistant per mu of ratio, reduces spraying pesticide 100 yuan of cost, per mu can increase by 350 yuan of economic benefit.
Embodiment 2：
1）It is sweet beautiful No. 2 with Sweet corn variety Shandong（P0）, glutinous corn variety SW22（P1）, high-resistance corn head smut kind Ji 465 （P2）, section 1 in high-resistance corn snout moth's larva kind（P3）, high-resistance corn helminthosporium maydis kind C103（P4）, it is neat that high-resistance corn embroiders sick kind 319（P5）For hybrid strain, make hybridization P0/P2, P0/P3, P0/P4, P0/P5, P1/P2, P1/P3, P1/P4, P1/P5, obtain 8 Individual hybridization F1, respectively with F1-P02、F1-P03、F1-P04、F1-P05 、F1-P12、F1-P13、F1-P14、F1- P15 is represented；
2）Plantation F1-P02、F1-P03、F1-P04、F1-P05、F1-P12、F1-P13、F1-P14、F1- P15 is each 10 plants, florescence Bagging selfing, obtains F2For seed F2-P02、F2-P03、F2-P04、F2-P05 、F2-P12、F2-P13、F2-P14、F2-P15；
3）F is planted respectively2For seed, each 50 plants of combination plantation, and Resistant identification is carried out, each combination only identifies which is corresponding Resistant parent institute disease and insect resistance species, filters out excellent 8 baggings of individual plant of high disease and insect resistance, leaf morphology and yield traits certainly Hand over, maturity period individual plant sowing, F2-P02、F2-P03、F2-P04、F2Seed on-P05 plant carries out sugariness identification, F2-P12、 F2-P13、F2-P14、F2Seed on-P15 plant carries out waxy indentification, and performance sugariness proterties or glutinous is selected in each combination respectively The seed of property proterties is each 20, obtains F3For seed F3-P02、F3-P03、F3-P04、F3-P05 、F3-P12、F3-P13、F3-P14、 F3-P15；
4）Plantation F3For seed, florescence makees hybridization F3-P02/F3-P03、F3-P04/F3-P05、F3-P12/F3-P13、F3- P14/F3- P15, must hybridize F1, respectively with F1-P023、F1-P045 、F1-P123、F1- P145 is represented；
5）Plantation F1-P023、F1-P045、F1-P123、F1- P145, bagging selfing obtain F2For seed F2-P023、F2-P045、F2- P123、F2-P145；
6）Plantation F2-P023、F2-P045、F2-P123、F2- P145 is each 40 plants, and carries out Resistant identification, and each combination is only reflected Fixed its corresponding 2 resistant parents institute disease and insect resistance species, filters out the excellent list of high disease and insect resistance, leaf morphology and yield traits The each 5 bagging selfings of strain, maturity period individual plant sowing, F2-P023、F2Seed on-P045 plant carries out sugariness identification, F2- P123、F2Seed on-P145 plant carries out waxy indentification, and performance sugariness proterties or waxy proterties are selected in each combination respectively Seed is each 20, obtains F3For seed F3-P023、F3-P045、F3-P123、F3-P145；
7）Plantation parent F3-P023、F3-P045、F3-P123、F3- P145 seeds, florescence make hybridization F3-P023/F3-P045、 F3-P123/F3- P145, must reestablish diplomatic relations F1, with F1-02345、F1- 12345 represent；
8）Plantation F1-02345、F1- 12345, florescence makees hybridization F1-02345/F1- 12345, obtain F1, with F1- 012345 represents；
9）Plantation F1- 012345, bagging selfing obtains F2- 012345, plant F2- 012345 totally 70 plants, and Resistant identification is carried out, The whole 4 resistant parent institute disease and insect resistance species of identification, filter out high anti-4 kinds of pest and disease damages, leaf morphology and yield traitses excellent 10 bagging selfings of individual plant, maturity period individual plant sowing, seed carry out sugariness and waxy indentification, select while showing sugariness proterties With 20, the seed of waxy proterties, F is obtained3For seed F3-012345；
10）Plantation F3For seed F3- 012345 totally 20 plants, and Resistant identification is carried out, all 4 resistant parents institutes are disease-resistant for identification Insect pest species, filters out high 5 bagging selfings of individual plant for resisting 4 kinds of pest and disease damages, leaf morphology and yield traitses excellent, maturity period list Strain sowing, obtains F4For seed F4-012345；
11）Strain plants F4For seed F4- 012345 totally 5 strains, each 30 plants of strain plantation, the maturity period carries out leaf morphology Identify with yield traits, screen 1 most excellent strain of leaf morphology and yield traits, therefrom select 3 elite seedling baggings Selfing, i.e. incubation leaf morphology, agronomy and yield traits are excellent, the high sweet glutinous maize self-bred line for resisting 4 kinds of pest and disease damages.
High anti-sweet-waxy maizes kind increases yield 20% because of the common sweet-waxy maizes kind of disease-resistant per mu of ratio, reduces spraying pesticide 150 yuan of cost, per mu can increase by 400 yuan of economic benefit.
Embodiment 3：
1）It is sweet beautiful No. 2 with Sweet corn variety Shandong（P0）, glutinous corn variety Shanghai is beautiful glutinous No. 1（P1）, high-resistance corn head smut kind Mo17（P2）, section 1 in high-resistance corn snout moth's larva kind（P3）, high-resistance corn helminthosporium maydis kind C103（P4）, the sick product of high-resistance corn embroidery Plant neat 319（P5）For hybrid strain, make hybridization P0/P2, P0/P3, P0/P4, P0/P5, P1/P2, P1/P3, P1/P4, P1/ P5, obtains 8 hybridization F1, respectively with F1-P02、F1-P03、F1-P04、F1-P05 、F1-P12、F1-P13、F1-P14、F1- P15 tables Show；
2）Plantation F1-P02、F1-P03、F1-P04、F1-P05、F1-P12、F1-P13、F1-P14、F1- P15 is each 10 plants, florescence Bagging selfing, obtains F2For seed F2-P02、F2-P03、F2-P04、F2-P05 、F2-P12、F2-P13、F2-P14、F2-P15；
3）F is planted respectively2For seed, each 45 plants of combination plantation, and Resistant identification is carried out, each combination only identifies which is corresponding Resistant parent institute disease and insect resistance species, filters out excellent 7 baggings of individual plant of high disease and insect resistance, leaf morphology and yield traits certainly Hand over, maturity period individual plant sowing, F2-P02、F2-P03、F2-P04、F2Seed on-P05 plant carries out sugariness identification, F2-P12、 F2-P13、F2-P14、F2Seed on-P15 plant carries out waxy indentification, and performance sugariness proterties or glutinous is selected in each combination respectively The seed of property proterties is each 20, obtains F3For seed F3-P02、F3-P03、F3-P04、F3-P05 、F3-P12、F3-P13、F3-P14、 F3-P15；
4）Plantation F3For seed, florescence makees hybridization F3-P02/F3-P03、F3-P04/F3-P05、F3-P12/F3-P13、F3- P14/F3- P15, must hybridize F1, respectively with F1-P023、F1-P045 、F1-P123、F1- P145 is represented；
5）Plantation F1-P023、F1-P045、F1-P123、F1- P145, bagging selfing obtain F2For seed F2-P023、F2-P045、F2- P123、F2-P145；
6）Plantation F2-P023、F2-P045、F2-P123、F2- P145 is each 40 plants, and carries out Resistant identification, and each combination is only reflected Fixed its corresponding 2 resistant parents institute disease and insect resistance species, filters out the excellent list of high disease and insect resistance, leaf morphology and yield traits The each 5 bagging selfings of strain, maturity period individual plant sowing, F2-P023、F2Seed on-P045 plant carries out sugariness identification, F2- P123、F2Seed on-P145 plant carries out waxy indentification, and performance sugariness proterties or waxy proterties are selected in each combination respectively Seed is each 20, obtains F3For seed F3-P023、F3-P045、F3-P123、F3-P145；
7）Plantation parent F3-P023、F3-P045、F3-P123、F3- P145 seeds, florescence make hybridization F3-P023/F3-P045、 F3-P123/F3- P145, must reestablish diplomatic relations F1, with F1-02345、F1- 12345 represent；
8）Plantation F1-02345、F1- 12345, florescence makees hybridization F1-02345/F1- 12345, obtain F1, with F1- 012345 represents；
9）Plantation F1- 012345, bagging selfing obtains F2- 012345, plant F2- 012345 totally 65 plants, and Resistant identification is carried out, The whole 4 resistant parent institute disease and insect resistance species of identification, filter out high anti-4 kinds of pest and disease damages, leaf morphology and yield traitses excellent 9 bagging selfings of individual plant, maturity period individual plant sowing, seed carry out sugariness and waxy indentification, select while showing sugariness proterties With 20, the seed of waxy proterties, F is obtained3For seed F3-012345；
10）Plantation F3For seed F3- 012345 totally 20 plants, and Resistant identification is carried out, all 4 resistant parents institutes are disease-resistant for identification Insect pest species, filters out high 5 bagging selfings of individual plant for resisting 4 kinds of pest and disease damages, leaf morphology and yield traitses excellent, maturity period list Strain sowing, obtains F4For seed F4-012345；
11）Strain plants F4For seed F4- 012345 totally 5 strains, each 30 plants of strain plantation, the maturity period carries out leaf morphology Identify with yield traits, screen 1 most excellent strain of leaf morphology and yield traits, therefrom select 2 elite seedling baggings Selfing, i.e. incubation leaf morphology, agronomy and yield traits are excellent, the high sweet glutinous maize self-bred line for resisting 4 kinds of pest and disease damages.
High anti-sweet-waxy maizes kind increases yield 17% because of the common sweet-waxy maizes kind of disease-resistant per mu of ratio, reduces spraying pesticide 130 yuan of cost, per mu can increase by 380 yuan of economic benefit.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with Modification, should all belong to the covering scope of the present invention.

Claims (2)

1. a kind of selection of high anti-sweet-waxy maizes kind, it is characterised in that the selection includes following methods with step Suddenly：
1）With Sweet corn variety P0, glutinous corn variety P1, high-resistance corn head smut kind P2, high-resistance corn snout moth's larva kind P3, height It is hybrid strain that anti-corn southern leaf blight kind P4, high-resistance corn embroider sick kind P5, makees hybridization P0/P2, P0/P3, P0/P4, P0/ P5, P1/P2, P1/P3, P1/P4, P1/P5, obtain 8 hybridization F1, respectively with F1-P02、F1-P03、F1-P04、F1-P05 、F1- P12、F1-P13、F1-P14、F1- P15 is represented；
2）Plantation F1-P02、F1-P03、F1-P04、F1-P05 、F1-P12、F1-P13、F1-P14、F1- P15 is each 10 plants, florescence Bagging selfing, obtains F2For seed F2-P02、F2-P03、F2-P04、F2-P05 、F2-P12、F2-P13、F2-P14、F2-P15；
3）F is planted respectively2For seed, each combination plantation 40-50 strain, and Resistant identification is carried out, its phase is only identified in each combination Resistant parent institute disease and insect resistance species is answered, the excellent individual plant of high disease and insect resistance, leaf morphology and yield traits is filtered out each 6-8 Bagging selfing, maturity period individual plant sowing, F2-P02、F2-P03、F2-P04、F2Seed on-P05 plant carries out sugariness identification, F2- P12、F2-P13、F2-P14、F2Seed on-P15 plant carries out waxy indentification, and performance sugariness proterties is selected in each combination respectively Or the seed of waxy proterties is each 20, obtains F3For seed F3-P02、F3-P03、F3-P04、F3-P05 、F3-P12、F3-P13、F3- P14、F3-P15；
4）Plantation F3For seed, florescence makees hybridization F3-P02/F3-P03、F3-P04/F3-P05、F3-P12/F3-P13、F3-P14/ F3- P15, must hybridize F1, respectively with F1-P023、F1-P045 、F1-P123、F1- P145 is represented；
5）Plantation F1-P023、F1-P045 、F1-P123、F1- P145, bagging selfing obtain F2For seed F2-P023、F2-P045、F2- P123、F2-P145；
6）Plantation F2-P023、F2-P045、F2-P123、F2- P145 is each 40 plants, and carries out Resistant identification, and each combination is only identified Its corresponding 2 resistant parents institute disease and insect resistance species, filters out the excellent individual plant of high disease and insect resistance, leaf morphology and yield traits Each 5 bagging selfings, maturity period individual plant sowing, F2-P023、F2Seed on-P045 plant carries out sugariness identification, F2-P123、 F2Seed on-P145 plant carries out waxy indentification, and the seed of performance sugariness proterties or waxy proterties is selected in each combination respectively Each 20, obtain F3For seed F3-P023、F3-P045、F3-P123、F3-P145；
7）Plantation parent F3-P023、F3-P045、F3-P123、F3- P145 seeds, florescence make hybridization F3-P023/F3-P045、 F3-P123/F3- P145, must reestablish diplomatic relations F1, with F1-02345、F1- 12345 represent；
8）Plantation F1-02345、F1- 12345, florescence makees hybridization F1-02345/F1- 12345, obtain F1, with F1- 012345 represents；
9）Plantation F1- 012345, bagging selfing obtains F2- 012345, plant F2- 012345 common 60-70 strains, and carry out Resistant mirror Fixed, the whole 4 resistant parent institute disease and insect resistance species of identification filter out high anti-4 kinds of pest and disease damages, leaf morphology and yield traitses excellent Different 8-10 bagging selfing of individual plant, maturity period individual plant sowing, seed carry out sugariness and waxy indentification, select while showing sugariness 20, the seed of proterties and waxy proterties, obtains F3For seed F3-012345；
10）Plantation F3For seed F3- 012345 totally 20 plants, and Resistant identification is carried out, all 4 resistant parents institutes are disease-resistant for identification Insect pest species, filters out high 5 bagging selfings of individual plant for resisting 4 kinds of pest and disease damages, leaf morphology and yield traitses excellent, maturity period list Strain sowing, obtains F4For seed F4-012345；
11）Strain plants F4For seed F4- 012345 totally 5 strains, each 30 plants of strain plantation, the maturity period carries out leaf morphology Identify with yield traits, screen 1 most excellent strain of leaf morphology and yield traits, therefrom select 2-3 elite seedling set Bag selfing, i.e. incubation leaf morphology, agronomy and yield traits are excellent, the high sweet glutinous maize self-bred line for resisting 4 kinds of pest and disease damages.
2. selection according to claim 1, it is characterised in that Sweet corn variety is Shandong sweet beautiful No. 1 or Shandong sweet beautiful 2 Number, glutinous corn variety is that Shanghai is beautiful glutinous No. 1 or SW22, and high-resistance corn head smut kind is Mo17 or Ji 465, high-resistance corn snout moth's larva product It is middle section 1 to plant, and high-resistance corn helminthosporium maydis kind is C103, and it is neat 319 that high-resistance corn embroiders sick kind.
CN201610958615.4A 2016-10-28 2016-10-28 Method for selecting seeds of high sweet-resistant waxy corn varieties Pending CN106508665A (en)

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CN102217524A (en) * 2011-05-11 2011-10-19 南京市蔬菜科学研究所 Breeding method for sweet glutinous maize self-bred line
US20160135402A1 (en) * 2014-11-13 2016-05-19 Seminis Vegetable Seeds, Inc. Sweet corn hybrid sv1446sd and parents thereof
CN105941137A (en) * 2016-07-07 2016-09-21 福建农林大学 Breeding method of broad-resistance green soybean variety

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Publication number Priority date Publication date Assignee Title
CN1930950A (en) * 2006-07-20 2007-03-21 王志刚 Breeding process of fruit-vegetable type sweet glutinous hybrid corn
CN102217524A (en) * 2011-05-11 2011-10-19 南京市蔬菜科学研究所 Breeding method for sweet glutinous maize self-bred line
US20160135402A1 (en) * 2014-11-13 2016-05-19 Seminis Vegetable Seeds, Inc. Sweet corn hybrid sv1446sd and parents thereof
CN105941137A (en) * 2016-07-07 2016-09-21 福建农林大学 Breeding method of broad-resistance green soybean variety

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Application publication date: 20170322