CN104488702B - The selection of the glutinous two-line sterile line of purple leaf upright spike length fringe neck - Google Patents
The selection of the glutinous two-line sterile line of purple leaf upright spike length fringe neck Download PDFInfo
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- CN104488702B CN104488702B CN201510019862.3A CN201510019862A CN104488702B CN 104488702 B CN104488702 B CN 104488702B CN 201510019862 A CN201510019862 A CN 201510019862A CN 104488702 B CN104488702 B CN 104488702B
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Abstract
The invention discloses the selection of the glutinous two-line sterile line of a kind of purple leaf upright spike length fringe neck, belong to Special rice breeding technical field.This method with purple leaf paddy rice, erect head paddy rice, long fringe neck paddy rice and glutinous two-line sterile line for parent, by hybridization, polymerization of reestablishing diplomatic relations and backcross, purple leaf, erect head, long fringe neck and these 4 kinds of proterties glutinous are aggregated in two-line sterile line material, backcross through many generations and screen, be bred as economical character, sterile phase conversion temperature, sterility excellence, have the two-line sterile line of purple leaf, erect head, long fringe neck and waxy 4 kinds of special beneficial characteristics, this glutinous rice two-line sterile line has that purity is high, hybrid seed yield is high, beat the advantages such as gibberellin less.
Description
Technical field
The present invention is specifically related to the selection of the glutinous two-line sterile line of a kind of purple leaf upright spike length fringe neck, belongs to Special rice breeding technical field.
Background technology
Paddy rice, as the first cereal crops of China, makes a great contribution to solution Food Security in China and raising people ' s health level.Glutinous rice is one of traditional grain crop of China, and be also the important source material that non-staple food is produced, the seed selection of glutinous rice has broad application prospects.At present, the glutinous rice variety in production is still based on conventional glutinous rice, and waxy hybrid rice combination is less, and the waxy hybrid rice combination identity of seed selection to assembly high yield and high quality of glutinous rice two-line sterile line is great.Two-line sterile line also exists the defect of inheritance Bao Sui, seed produces must use gibberellin, not only increase the cost of seed produces, also create environmental pollution, seed selection long fringe neck two-line sterile line can reach heredity to be removed or alleviates Bao Sui, use no or little gibberellin, thus the seed produces cost of hybrid rice is declined, seed quality improves and reduces environmental pollution.The rice panicle type group structure of erect head type is reasonable, and the ecological conditions such as canopy intensity of illumination, temperature, humidity are superior, is conducive to improving fertilizer resistance anti-lodging, also helps pollen transmission, improve outcrossing seed-setting rate.Two-line sterile line of rice occurs replying because fertility is subject to the impact of anomalous weather, causes hybrid F
1purity decline in various degree, production of hybrid seeds production risk coefficient is higher.Using purple leaf gene by hybridization method transformation to two-line sterile line as mark property, mix plant to remove, can greatly improve hybrid F
1purity.Purple leaf, erect head, long fringe neck and these 4 kinds of proterties glutinous are aggregated in two-line sterile line simultaneously, the glutinous rice two-line sterile line possessing multiple advantage can be bred as.
Summary of the invention
The object of the present invention is to provide the selection of the glutinous two-line sterile line of a kind of purple leaf upright spike length fringe neck, thus improve seed production purity, the hybrid seed yield of glutinous two-line sterile line, reduce breeding cost.This method with purple leaf paddy rice, erect head paddy rice, long fringe neck paddy rice and glutinous two-line sterile line for parent, by hybridization, polymerization of reestablishing diplomatic relations and backcross, purple leaf, erect head, long fringe neck and these 4 kinds of proterties glutinous are aggregated in two-line sterile line material, backcross through many generations and screen, be bred as economical character, sterile phase conversion temperature, sterility excellence, there is the two-line sterile line of purple leaf, erect head, long fringe neck and waxy 4 kinds of special beneficial characteristics.
For reaching above object, the present invention by the following technical solutions:
1) select purple leaf rice varieties (A), erect head rice varieties (B), long fringe neck rice varieties (C), waxy rice two-line sterile line (D), make hybridization A/B and D/C respectively, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, must reestablish diplomatic relations F
1, with F
1-ABCDrepresent;
3) F is planted
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1200-1400 strain, carries out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, maturing stage is individual plant sowing respectively, obtains F
3-ABCDseed;
4) by 3) gained F
3-ABCDseed shells out rough, selects the brown rice grain with glutinous rice proterties;
5) strain plantation 4) gained has the F of purple leaf, long fringe neck, erect head and waxy shape
3-ABCDsingle-strain seed, each strain plantation 40-50 strain, plant glutinous two-line sterile line D simultaneously, flowering stage is identified each strain, select to show purple leaf, long fringe neck with erect head proterties and without the strain be separated simultaneously, the individual plant 5-10 of economical character excellence is therefrom selected to make female parent with glutinous two-line sterile line D() hybridize, must F be hybridized
1in generation, with SF
1-ABCDrepresent;
6) 5 are planted) gained SF
1-ABCDseed, selfing obtains SF
2-ABCD, strain plantation SF
2-ABCD, colony, in 500-800 strain, carries out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, maturing stage is individual plant sowing respectively, obtains SF
3-ABCDseed;
7) 6 are planted) gained SF
3-ABCDseed, selects the SF of economical character excellence flowering stage
3-ABCDindividual plant 5 and glutinous two-line sterile line D(make female parent) backcross, obtain 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, colony is in 500-600 strain, carry out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select to have that long fringe neck and erect head proterties, economical character are excellent, the most similar to glutinous two-line sterile line D, pollen sterility individual plant 50 strain thoroughly listing mark simultaneously, shift-in temperature is that the climatic cabinate of 23 degree cuts pocket and regenerates, flowering stage observes pollen fertility, selects individual plant 10 sowings that pollen fertility is good, ripening rate is high;
8) strain plantation 7) selected 10 individual plants, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, select economical character similar to glutinous two-line sterile line D, there is purple leaf, long fringe neck and erect head proterties, sterile phase pollen sterility is thorough and namely sterile conversion temperature is close with glutinous two-line sterile line D single-plant propagation seed is bred as the purple leaf upright spike length glutinous two-line sterile line of fringe neck " ZZewxS ".
Purple leaf rice varieties (A) is IR1552, erect head rice varieties (B) opens book label etc. for 259(. the genetic analysis of the cultivation of long-grained nonglutinous rice erect head mutant, qualification and mutant character thereof. and nuclear agricultural science report, 2007,21 (3): 209-211), long fringe neck rice varieties (C) for eR127, II-32eB1 or association blue or green early eB1, waxy rice two-line sterile line (D) be bright glutinous S-1.
beneficial effect of the present invention:
Effect of the present invention is respectively the gene controlling purple leaf, erect head, long fringe neck and these 4 kinds of proterties glutinous to be backcrossed and triage techniques through many generation hybridization, be aggregated in same two-line sterile line of rice, be bred as economical character, sterile phase conversion temperature, sterility excellence, have the two-line sterile line of purple leaf, erect head, long fringe neck and waxy 4 kinds of special beneficial characteristics, this glutinous rice two-line sterile line has that male sterile line purity is high, hybrid seed yield is high, hybrid F
1purity is high, the advantage such as gibberellin, decreasing pollution is beaten in the production of hybrid seeds less, and every mu of seed farm can reduce costs 80-100 unit, increase yield 5-10%.
Embodiment
Embodiment 1:
1) select purple leaf rice varieties IR1552(A), erect head rice varieties 259(B), long fringe neck rice varieties eR127(C), the bright glutinous S-1(D of waxy rice two-line sterile line), respectively do hybridization A/B and D/C, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, must reestablish diplomatic relations F
1, with F
1-ABCDrepresent;
3) F is planted
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1200 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains F
3-ABCDseed;
4) by 3) gained F
3-ABCDseed shells out rough, selects the brown rice grain with glutinous rice proterties;
5) strain plantation 4) gained has the F of purple leaf, long fringe neck, erect head and waxy shape
3-ABCDsingle-strain seed, each strain plants 40 strains, plant the bright glutinous S-1 of glutinous two-line sterile line simultaneously, flowering stage is identified each strain, select to show purple leaf, long fringe neck with erect head proterties and without the strain be separated simultaneously, the individual plant 5 of economical character excellence and the bright glutinous S-1(of glutinous two-line sterile line is therefrom selected to make female parent) hybridize, must F be hybridized
1in generation, with SF
1-ABCDrepresent;
6) 5 are planted) gained SF
1-ABCDseed, selfing obtains SF
2-ABCD, strain plantation SF
2-ABCD, colony, in 500 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains SF
3-ABCDseed;
7) 6 are planted) gained SF
3-ABCDseed, selects the SF of economical character excellence flowering stage
3-ABCDindividual plant 5 and the bright glutinous S-1(of glutinous two-line sterile line make female parent) backcross, obtain 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, colony is in 500 strains, carry out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select to have that long fringe neck and erect head proterties, economical character are excellent, pollen sterility thoroughly individual plant 50 strain listing mark the most similar to the bright glutinous S-1 of glutinous two-line sterile line simultaneously, shift-in temperature is that the climatic cabinate of 23 degree cuts pocket and regenerates, flowering stage observes pollen fertility, selects individual plant 10 sowings that pollen fertility is good, ripening rate is high;
8) strain plantation 7) selected 10 individual plants, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, select economical character similar to the bright glutinous S-1 of glutinous two-line sterile line, there is purple leaf, long fringe neck and erect head proterties, sterile phase pollen sterility is thorough and namely sterile conversion temperature is close with the bright glutinous S-1 of glutinous two-line sterile line single-plant propagation seed is bred as the purple leaf upright spike length glutinous two-line sterile line of fringe neck " ZZewxS-1 ".
Glutinous rice two-line sterile line " ZZewxS-1 " has that male sterile line purity is high, hybrid seed yield is high, hybrid F
1purity is high, the advantage such as gibberellin, decreasing pollution is beaten in the production of hybrid seeds less, and every mu of seed farm can reduce costs 80 yuan, increase yield 5%.
Embodiment 2:
1) select purple leaf rice varieties IR1552(A), erect head rice varieties 259(B), the blue or green early eB1(C of long fringe neck rice varieties association), the bright glutinous S-1(D of waxy rice two-line sterile line), make hybridization A/B and D/C respectively, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, must reestablish diplomatic relations F
1, with F
1-ABCDrepresent;
3) F is planted
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1300 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains F
3-ABCDseed;
4) by 3) gained F
3-ABCDseed shells out rough, selects the brown rice grain with glutinous rice proterties;
5) strain plantation 4) gained has the F of purple leaf, long fringe neck, erect head and waxy shape
3-ABCDsingle-strain seed, each strain plants 45 strains, plant the bright glutinous S-1 of glutinous two-line sterile line simultaneously, flowering stage is identified each strain, select to show purple leaf, long fringe neck with erect head proterties and without the strain be separated simultaneously, the individual plant 8 of economical character excellence and the bright glutinous S-1(of glutinous two-line sterile line is therefrom selected to make female parent) hybridize, must F be hybridized
1in generation, with SF
1-ABCDrepresent;
6) 5 are planted) gained SF
1-ABCDseed, selfing obtains SF
2-ABCD, strain plantation SF
2-ABCD, colony, in 600 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains SF
3-ABCDseed;
7) 6 are planted) gained SF
3-ABCDseed, selects the SF of economical character excellence flowering stage
3-ABCDindividual plant 5 and the bright glutinous S-1(of glutinous two-line sterile line make female parent) backcross, obtain 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, colony is in 550 strains, carry out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select to have that long fringe neck and erect head proterties, economical character are excellent, pollen sterility thoroughly individual plant 50 strain listing mark the most similar to the bright glutinous S-1 of glutinous two-line sterile line simultaneously, shift-in temperature is that the climatic cabinate of 23 degree cuts pocket and regenerates, flowering stage observes pollen fertility, selects individual plant 10 sowings that pollen fertility is good, ripening rate is high;
8) strain plantation 7) selected 10 individual plants, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, select economical character similar to the bright glutinous S-1 of glutinous two-line sterile line, there is purple leaf, long fringe neck and erect head proterties, sterile phase pollen sterility is thorough and namely sterile conversion temperature is close with the bright glutinous S-1 of glutinous two-line sterile line single-plant propagation seed is bred as the purple leaf upright spike length glutinous two-line sterile line of fringe neck " ZZewxS-2 ".
Glutinous rice two-line sterile line " ZZewxS-2 " has that male sterile line purity is high, hybrid seed yield is high, hybrid F
1purity is high, the advantage such as gibberellin, decreasing pollution is beaten in the production of hybrid seeds less, and every mu of seed farm can reduce costs 90 yuan, increase yield 8%.
Embodiment 3:
1) select purple leaf rice varieties IR1552(A), erect head rice varieties 259(B), long fringe neck rice varieties II-32eB1(C), the bright glutinous S-1(D of waxy rice two-line sterile line), respectively do hybridization A/B and D/C, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, must reestablish diplomatic relations F
1, with F
1-ABCDrepresent;
3) F is planted
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1400 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains F
3-ABCDseed;
4) by 3) gained F
3-ABCDseed shells out rough, selects the brown rice grain with glutinous rice proterties;
5) strain plantation 4) gained has the F of purple leaf, long fringe neck, erect head and waxy shape
3-ABCDsingle-strain seed, each strain plantation 40-50 strain, plant the bright glutinous S-1 of glutinous two-line sterile line simultaneously, flowering stage is identified each strain, select to show purple leaf, long fringe neck with erect head proterties and without the strain be separated simultaneously, the individual plant 10 of economical character excellence and the bright glutinous S-1(of glutinous two-line sterile line is therefrom selected to make female parent) hybridize, must F be hybridized
1in generation, with SF
1-ABCDrepresent;
6) 5 are planted) gained SF
1-ABCDseed, selfing obtains SF
2-ABCD, strain plantation SF
2-ABCD, colony, in 800 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains SF
3-ABCDseed;
7) 6 are planted) gained SF
3-ABCDseed, selects the SF of economical character excellence flowering stage
3-ABCDindividual plant 5 and the bright glutinous S-1(of glutinous two-line sterile line make female parent) backcross, obtain 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, colony is in 600 strains, carry out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select to have that long fringe neck and erect head proterties, economical character are excellent, pollen sterility thoroughly individual plant 50 strain listing mark the most similar to the bright glutinous S-1 of glutinous two-line sterile line simultaneously, shift-in temperature is that the climatic cabinate of 23 degree cuts pocket and regenerates, flowering stage observes pollen fertility, selects individual plant 10 sowings that pollen fertility is good, ripening rate is high;
8) strain plantation 7) selected 10 individual plants, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, select economical character similar to the bright glutinous S-1 of glutinous two-line sterile line, there is purple leaf, long fringe neck and erect head proterties, sterile phase pollen sterility is thorough and namely sterile conversion temperature is close with the bright glutinous S-1 of glutinous two-line sterile line single-plant propagation seed is bred as the purple leaf upright spike length glutinous two-line sterile line of fringe neck " ZZewxS-3 ".
Glutinous rice two-line sterile line " ZZewxS-3 " has that male sterile line purity is high, hybrid seed yield is high, hybrid F
1purity is high, the advantage such as gibberellin, decreasing pollution is beaten in the production of hybrid seeds less, and every mu of seed farm can reduce costs 100 yuan, increase yield 10%.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. the selection of the glutinous two-line sterile line of purple leaf upright spike length fringe neck, is characterized in that: described selection comprises the following steps:
1) select purple leaf rice varieties (A), erect head rice varieties (B), long fringe neck rice varieties (C), waxy rice two-line sterile line (D), make hybridization A/B and D/C respectively, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, must reestablish diplomatic relations F
1, with F
1-ABCDrepresent;
3) F is planted
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1200-1400 strain, carries out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, maturing stage is individual plant sowing respectively, obtains F
3-ABCDseed;
4) by 3) gained F
3-ABCDseed shells out rough, selects the brown rice grain with glutinous rice proterties;
5) strain plantation 4) gained has the F of purple leaf, long fringe neck, erect head and waxy shape
3-ABCDsingle-strain seed, each strain plantation 40-50 strain, plant glutinous two-line sterile line D simultaneously, flowering stage is identified each strain, select to show purple leaf, long fringe neck with erect head proterties and without the strain be separated simultaneously, therefrom select the individual plant 5-10 of economical character excellence to hybridize with glutinous two-line sterile line D, glutinous two-line sterile line D does maternal, must hybridize F
1in generation, with SF
1-ABCDrepresent;
6) 5 are planted) gained SF
1-ABCDseed, selfing obtains SF
2-ABCD, strain plantation SF
2-ABCD, colony, in 500-800 strain, carries out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, maturing stage is individual plant sowing respectively, obtains SF
3-ABCDseed;
7) 6 are planted) gained SF
3-ABCDseed, selects the SF of economical character excellence flowering stage
3-ABCDindividual plant 5 and glutinous two-line sterile line D backcross, and glutinous two-line sterile line D does maternal, obtains 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, colony is in 500-600 strain, carry out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select to have that long fringe neck and erect head proterties, economical character are excellent, the most similar to glutinous two-line sterile line D, pollen sterility individual plant 50 strain thoroughly listing mark simultaneously, shift-in temperature is that the climatic cabinate of 23 degree cuts pocket and regenerates, flowering stage observes pollen fertility, selects individual plant 10 sowings that pollen fertility is good, ripening rate is high;
8) strain plantation 7) selected 10 individual plants, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, select economical character similar to glutinous two-line sterile line D, there is purple leaf, long fringe neck and erect head proterties, sterile phase pollen sterility is thorough and namely sterile conversion temperature is close with glutinous two-line sterile line D single-plant propagation seed is bred as the purple leaf upright spike length glutinous two-line sterile line of fringe neck " ZZewxS ".
2. the selection of the glutinous two-line sterile line of purple leaf according to claim 1 upright spike length fringe neck, is characterized in that: purple leaf rice varieties (A) is IR1552, erect head rice varieties (B) is 259, for eR127, II-32eB1 or association green grass or young crops, early eB1, waxy rice two-line sterile line (D) are bright glutinous S-1 to long fringe neck rice varieties (C).
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CN105706913B (en) * | 2016-03-09 | 2018-01-12 | 福建农林大学 | A kind of selection of feeding upland rice two-line sterile line |
CN106993528A (en) * | 2017-04-06 | 2017-08-01 | 中国水稻研究所 | A kind of dense cluster type two-line sterile line of rice selection |
CN109964807B (en) * | 2019-03-29 | 2022-03-25 | 福建省农业科学院水稻研究所 | Method for cultivating upright ear type black glutinous rice three-line sterile line |
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CN103229712A (en) * | 2013-05-06 | 2013-08-07 | 福建农林大学 | Breeding method of polyclonal antibody cold-resistant two-line sterile line |
CN103891605A (en) * | 2014-04-04 | 2014-07-02 | 福建农林大学 | Breeding method for disease-resistant functional rice two-line sterile line |
CN104137765A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of high-stress resistance elongated upper internode two-line genic male sterile line |
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CN103229712A (en) * | 2013-05-06 | 2013-08-07 | 福建农林大学 | Breeding method of polyclonal antibody cold-resistant two-line sterile line |
CN103891605A (en) * | 2014-04-04 | 2014-07-02 | 福建农林大学 | Breeding method for disease-resistant functional rice two-line sterile line |
CN104137765A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of high-stress resistance elongated upper internode two-line genic male sterile line |
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