CN104642101A - Breeding method of two-line sterility line with special character - Google Patents
Breeding method of two-line sterility line with special character Download PDFInfo
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Abstract
The invention discloses a breeding method of a two-line sterility line with a special character, and belongs to the technical field of special rice breeding. The method comprises the following steps: selecting four special rice varieties respectively with glabrous property, premature senility resistance, endurable storage and yellow seed coat glutinous property as materials, hybridizing, multiple-crossing and backcrossing transforming, and screening strains with the glabrous property, premature senility resistance, endurable storage, yellow seed coat, glutinous property, agronomic trait, yield character and sterility of the segregative generation, breeding the screened strains into a yellow glutinous rice two-line sterility line with glabrous property, premature senility resistance and endurable storage, assembling the yellow glutinous rice two-line sterility line with a restoring line with the same characters to breed the yellow glutinous two-line hybrid rice with the glabrous property, premature senility resistance and endurable storage. The hybrid rice is capable of resisting premature senility, endurable in storage, high in yield, easy to mechanically harvest, the rice paddy is yellow, and the rice is soft and good in taste, and rich in various nutrients.
Description
Technical field
The present invention is specifically related to a kind of selection with specialized character two-line sterile line, belongs to Special rice breeding technical field.
Background technology
Shell clever in common cultivated rice, blade without the type of hair, are called naked body rice by people.The rice varieties of the U.S. is the needs adapting to mechanization, and major part is all naked body rice, because its meter of matter is better, also has the characteristics such as specular removal, has the example successfully utilized in the conventional variety seed selection of China." naked body rice ", because whole body is without hair, sick worm not easily adheres to and invades, and performance has stronger resistant to diseases and insects, skin can be reduced itch and damage to skin when manual harvesting and threshing, be easy to mechanized harvest, add free from dust in man-hour, environmental pollution can be reduced, so also known as " environmental protection paddy rice ".Rice premature refers to that the phenomenon failed appears in the physiological function at the positions such as late growth stage of rice cauline leaf too early, and ripening period cauline leaf is withered and yellow, affects photosynthesis, causes production declining.Early ageing is caused by the characteristic of environment and kind, and a key factor of restriction Indica Rice output increased is exactly the early ageing phenomenon of late growth stage of rice at present, and the grain yield loss caused because of rice premature every year with regard to one, Jiangxi province reaches 10-15 hundred million kilograms.Often extend 1 day at rice maturity functional leaf and carry out photosynthesis, can increase by the output of about 1%.Solve the rice varieties that rice premature problem the best way is exactly seed selection premature senescence resistance.It is that puzzlement is produced, a difficult problem of accumulating and operating division that the shoddy change of rice in rice storage process, germination rate decline always, causes China's foodstuff preservation loss average out to 3.05% thus.Rice paddy seed also causes huge economic loss because vigor declines in storage, and can affect the upgrowth situation of percentage of seedgermination and seedling.Therefore, cultivate storage endurance new rice variety, the problems such as solution rice Deterioration are had great importance.Glutinous millet produces yellow rind by having yellow anthocyan in pericarp, and anthocyan is described as " the special effect element of life ", and glutinous millet is also rich in vitamin A and several amino acids, and nutrient composition content is high.Glutinous rice is nutritious, and mouthfeel is good, is the primary raw material manufacturing viscosity snack and various sweets.
By in above 4 kinds of proterties polymerization Introduced into Rice two-line sterile line, can be bred as and there is naked body, premature senescence resistance, the yellow glutinous rice two-line sterile line of storage endurance characteristic, naked body can be bred as with the restorer assembly with same proterties with it, premature senescence resistance, the glutinous two-line hybrid rice of Huang of storage endurance, this hybrid rice energy premature senescence resistance, storage endurance, output is high, be easy to mechanized harvest, rice is yellow, rice is soft, mouthfeel is good, be rich in multiple nutrients material, there is wide market prospects and Development volue, its market price than common rice height 3.5-4.5 doubly, popularizing planting is for the income improving vast kind of rice peasant, for the trophic level improving broad masses of the people, improve health and all there is certain meaning.
Summary of the invention
The object of the present invention is to provide a kind of selection with specialized character two-line sterile line, utilize the method can select the glutinous two-line sterile line of Huang of naked body, premature senescence resistance, storage endurance.
For achieving the above object, the present invention adopts following technical scheme:
The present invention selects naked body rice varieties " light 159 ", premature senescence resistance rice varieties " No. 62, grand round-grained rice ", storage endurance rice varieties " two autumns are short No. 1 " (Yu Liqin etc. the screening of Long-storage Rice Germplasm Resources. Agriculture in Jiangxi journal, 2008, 20 (4): 17-19), yellow glutinous rice variety " glutinous millet is glutinous " (Chen great Zhou, indica type glutinous millet Glutinous CMS line seed selection and utilize technology. Agriculture in Jiangxi journal, 1993, 5 (1): 7-20), two-line sterile line " 2018S ", " 6311S " and " C815S " is material, by hybridization, reestablish diplomatic relations and backcross transformation, and to segregative generation naked body, premature senescence resistance, storage endurance, yellow seed coat, waxy, economical character, the screening of yield traits and sterility etc., incubation has naked body, premature senescence resistance, the yellow glutinous rice two-line sterile line of storage endurance characteristic.
Concrete steps are as follows:
1) select naked body rice varieties " light 159 " (A), premature senescence resistance rice varieties " No. 62, grand round-grained rice " (B), storage endurance rice varieties " two autumns are short No. 1 " (C), yellow glutinous rice variety " glutinous millet is glutinous " (D), make hybridization A/B and C/D, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent, plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ F
1-CD, maturing stage sowing must be reestablished diplomatic relations F
1for seed, with F
1-ABCDrepresent;
2) F is planted
1-ABCDseed, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony 1100-1300 strain, carry out blade naked body character screening in the early tillering stage, the maturing stage carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 200-300 strain individual plant to gather in the crops seed (F
3-ABCD), every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation of future generation;
3) strain plantation 2) screen the F obtained
3-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 200-250 strain individual plant sowing (F of economical character, yield traits excellence
4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification (because naked body and waxy proterties are that Recessive genes controls, when showing this proterties, gene isozygotys, can not be separated again), screening retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation of future generation;
4) strain plantation 3) F of gained
4-ABCD, plant two-line sterile line S(two-line sterile line S is " 2018S ", " 6311S " and " C815S " simultaneously), select the strain of Agronomic excellence flowering stage and therefrom select elite seedling 5 strain, doing with two-line sterile line S maternal, making hybridization S/F
4-ABCD, plantation hybridization F
1in generation, is (with F
1-Srepresent), selfing sowing obtains F
2-S;
5) 4 are planted) F of gained
2-Scolony 800-900 strain, blade naked body character screening is carried out in the early tillering stage, maturing stage carries out premature senescence resistance screening, naked body premature senescence resistance individual plant 200-300 strain individual plant is selected to gather in the crops seed, every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation (F of future generation
3-S);
6) strain plantation 5) screen the F obtained
3-S, plant two-line sterile line S, flowering stage is done maternal with two-line sterile line S, backcross S/F simultaneously
3-S, must backcross BC
1f
1generation, plantation BC
1f
1in generation, selfing sowing obtains BC
1f
2, repeat step 5) and obtain BC
1f
3generation;
7) BC
1f
3generation-BC
1f
6in generation, presses pedigree method plantation, every Dai Jun carries out premature senescence resistance and storage endurance screening, upper menu strain is by strain plantation, therefrom select that plant cane is sturdy, (pollen is through microexamination for the sterile plant 50 of big panicle many grains per panicle again, completely sterile), cut shift-in climatic cabinate 23 degree regeneration after pocket, select pollen loose powder good, the individual plant sowing that ripening rate is high, at BC
1f
6select that population characteristics is stable not to be separated generation, sterility is stablized and namely the good strain sizing of Fertility al-teration is bred as the glutinous two-line sterile line of Huang with naked body, premature senescence resistance, storage endurance.
In above-mentioned Breeding Process, the evaluation and screening method of 4 kinds of specialized characters and step are: the screening of naked body proterties carries out the screening of blade naked body proterties in the early tillering stage by strain, listing mark has the rice strain of naked body proterties, premature senescence resistance screening selects rice straw sturdy in the maturing stage listing in rice strain, blade keeps the individual plant sowing of green not early ageing, gained seed numbering is dried, dry rear storage 20 days, every part of seed is got part and is carried out rough process, naked eyes select brown rice performance yellow and unseparated individual plant, its brown rice is carried out waxy indentification by grain IKI, screening retains the waxy and unseparated individual plant of performance, and its corresponding rough Some seeds that do not go out is carried out hot and humid artificial accelerated aging process, artificial accelerated aging processing method is dried 6 days through-18 DEG C of refrigerator freezings 12 days and 44 DEG C, baking oven by seed, again temperature 42 DEG C, artificial accelerated aging 22d is carried out in the climatic cabinate of humidity 88%, take out seed and carry out germination test, screening germination rate enters plantation of future generation higher than the individual plant of 78%.BC
1f
3generation-BC
1f
6dai Zhong, every step needing sowing, the flowering stage of its material plantation is all arranged in ear under two-line sterile line can educate phase temperature condition blooms, every need the step identified the sterile phase pollen sterility of material to be all arranged in the sterile phase temperature condition of two-line sterile line under ear and bloom, the procedure that the sterile conversion temperature of material is identified is carried out in climatic cabinate.
A kind of selection with specialized character two-line sterile line, the male sterile line of its seed selection has naked body proterties, premature senescence resistance, seed storage endurance, rice is yellow and be waxy, can be bred as the glutinous two-line hybrid rice of Huang of naked body, premature senescence resistance, storage endurance, this hybrid rice energy premature senescence resistance, storage endurance with it and the restorer assembly with same proterties, output is high, be easy to mechanized harvest, rice is yellow, and rice is soft, mouthfeel good, is rich in multiple nutrients material.
Beneficial effect of the present invention is: utilize the method can respectively naked body proterties, premature senescence resistance, storage endurance, yellow seed coat, waxy proterties imports in two-line sterile line, this male sterile line is utilized to go out high yield with the restorer energy assembly with same proterties, premature senescence resistance, storage endurance, be easy to mechanized harvest, rice is yellow, rice is soft, mouthfeel is good, be rich in the Special Rices kind of multiple nutrients material, this hybrid rice is than standard hybridization rice volume increase 5-7%, shelf time extends 7-8 month, nutrient composition content is high, rice is yellow, rice is soft glutinous, mouthfeel is good, there is good tonic effect, price is 3.5-4.5 times of general rice.
Embodiment
Embodiment 1:
1) select naked body rice varieties " light 159 " (A), premature senescence resistance rice varieties " No. 62, grand round-grained rice " (B), storage endurance rice varieties " two autumns are short No. 1 " (C), yellow glutinous rice variety " glutinous millet is glutinous " (D), make hybridization A/B and C/D, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent, plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ F
1-CD, maturing stage sowing must be reestablished diplomatic relations F
1for seed, with F
1-ABCDrepresent;
2) F is planted
1-ABCDseed, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony 1100 strain, carry out blade naked body character screening in the early tillering stage, the maturing stage carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 200 strain individual plant to gather in the crops seed (F
3-ABCD), every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation of future generation;
3) strain plantation 2) screen the F obtained
3-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 200 strain individual plant sowing (F of economical character, yield traits excellence
4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, screening retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation of future generation;
4) strain plantation 3) F of gained
4-ABCD, plant two-line sterile line " 2018S " simultaneously, select the strain of Agronomic excellence flowering stage and therefrom select elite seedling 5 strain, do with " 2018S " maternal, do to hybridize " 2018S "/F
4-ABCD, plantation hybridization F
1in generation, is (with F
1-Srepresent), selfing sowing obtains F
2-S;
5) 4 are planted) F of gained
2-Scolony 800 strain, blade naked body character screening is carried out in the early tillering stage, maturing stage carries out premature senescence resistance screening, naked body premature senescence resistance individual plant 200 strain individual plant is selected to gather in the crops seed, every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation (F of future generation
3-S);
6) strain plantation 5) screen the F obtained
3-S, plant " 2018S ", flowering stage is done maternal with " 2018S ", backcross " 2018S "/F simultaneously
3-S, must backcross BC
1f
1generation, plantation BC
1f
1in generation, selfing sowing obtains BC
1f
2, repeat step 5) and obtain BC
1f
3generation;
7) BC
1f
3generation-BC
1f
6in generation, presses pedigree method plantation, every Dai Jun carries out premature senescence resistance and storage endurance screening, upper menu strain is by strain plantation, therefrom select that plant cane is sturdy, (pollen is through microexamination for the sterile plant 50 of big panicle many grains per panicle again, completely sterile), cut shift-in climatic cabinate 23 degree regeneration after pocket, select pollen loose powder good, the individual plant sowing that ripening rate is high, at BC
1f
6select that population characteristics is stable not to be separated generation, sterility is stablized and namely the good strain sizing of Fertility al-teration is bred as the glutinous two-line sterile line of Huang " special 2018S " with naked body, premature senescence resistance, storage endurance.
In above-mentioned Breeding Process, the evaluation and screening method of 4 kinds of specialized characters and step are: the screening of naked body proterties carries out the screening of blade naked body proterties in the early tillering stage by strain, listing mark has the rice strain of naked body proterties, premature senescence resistance screening selects rice straw sturdy in the maturing stage listing in rice strain, blade keeps the individual plant sowing of green not early ageing, gained seed numbering is dried, dry rear storage 20 days, every part of seed is got part and is carried out rough process, naked eyes select brown rice performance yellow and unseparated individual plant, its brown rice is carried out waxy indentification by grain IKI, screening retains the waxy and unseparated individual plant of performance, and its corresponding rough Some seeds that do not go out is carried out hot and humid artificial accelerated aging process, artificial accelerated aging processing method is dried 6 days through-18 DEG C of refrigerator freezings 12 days and 44 DEG C, baking oven by seed, again temperature 42 DEG C, artificial accelerated aging 22d is carried out in the climatic cabinate of humidity 88%, take out seed and carry out germination test, screening germination rate enters plantation of future generation higher than the individual plant of 78%.BC
1f
3generation-BC
1f
6dai Zhong, every step needing sowing, the flowering stage of its material plantation is all arranged in ear under two-line sterile line can educate phase temperature condition blooms, every need the step identified the sterile phase pollen sterility of material to be all arranged in the sterile phase temperature condition of two-line sterile line under ear and bloom, the procedure that the sterile conversion temperature of material is identified is carried out in climatic cabinate.
" special 2018S " is utilized to go out high yield, premature senescence resistance, storage endurance with the restorer energy assembly with same proterties, be easy to mechanized harvest, rice is yellow, rice soft glutinous, mouthfeel good, be rich in the Special Rices kind of multiple nutrients material, this hybrid rice is than standard hybridization rice volume increase about 5%, shelf time extends about 7 months, nutrient composition content is high, and rice is yellow, rice soft glutinous, mouthfeel good, have good tonic effect, price is 3.5-4.5 times of general rice.
Embodiment 2:
1) select naked body rice varieties " light 159 " (A), premature senescence resistance rice varieties " No. 62, grand round-grained rice " (B), storage endurance rice varieties " two autumns are short No. 1 " (C), yellow glutinous rice variety " glutinous millet is glutinous " (D), make hybridization A/B and C/D, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent, plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ F
1-CD, maturing stage sowing must be reestablished diplomatic relations F
1for seed, with F
1-ABCDrepresent;
2) F is planted
1-ABCDseed, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony 1200 strain, carry out blade naked body character screening in the early tillering stage, the maturing stage carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 250 strain individual plant to gather in the crops seed (F
3-ABCD), every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation of future generation;
3) strain plantation 2) screen the F obtained
3-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 230 strain individual plant sowing (F of economical character, yield traits excellence
4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, screening retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation of future generation;
4) strain plantation 3) F of gained
4-ABCD, plant two-line sterile line " 6311S " simultaneously, select the strain of Agronomic excellence flowering stage and therefrom select elite seedling 5 strain, do with " 6311S " maternal, do to hybridize " 6311S "/F
4-ABCD, plantation hybridization F
1in generation, is (with F
1-Srepresent), selfing sowing obtains F
2-S;
5) 4 are planted) F of gained
2-Scolony 850 strain, blade naked body character screening is carried out in the early tillering stage, maturing stage carries out premature senescence resistance screening, naked body premature senescence resistance individual plant 250 strain individual plant is selected to gather in the crops seed, every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation (F of future generation
3-S);
6) strain plantation 5) screen the F obtained
3-S, plant " 6311S ", flowering stage is done maternal with " 6311S ", backcross " 6311S "/F simultaneously
3-S, must backcross BC
1f
1generation, plantation BC
1f
1in generation, selfing sowing obtains BC
1f
2, repeat step 5) and obtain BC
1f
3generation;
7) BC
1f
3generation-BC
1f
6in generation, presses pedigree method plantation, every Dai Jun carries out premature senescence resistance and storage endurance screening, upper menu strain is by strain plantation, therefrom select that plant cane is sturdy, (pollen is through microexamination for the sterile plant 50 of big panicle many grains per panicle again, completely sterile), cut shift-in climatic cabinate 23 degree regeneration after pocket, select pollen loose powder good, the individual plant sowing that ripening rate is high, at BC
1f
6select that population characteristics is stable not to be separated generation, sterility is stablized and namely the good strain sizing of Fertility al-teration is bred as the glutinous two-line sterile line of Huang " special 6311S " with naked body, premature senescence resistance, storage endurance.
In above-mentioned Breeding Process, the evaluation and screening method of 4 kinds of specialized characters and step are: the screening of naked body proterties carries out the screening of blade naked body proterties in the early tillering stage by strain, listing mark has the rice strain of naked body proterties, premature senescence resistance screening selects rice straw sturdy in the maturing stage listing in rice strain, blade keeps the individual plant sowing of green not early ageing, gained seed numbering is dried, dry rear storage 20 days, every part of seed is got part and is carried out rough process, naked eyes select brown rice performance yellow and unseparated individual plant, its brown rice is carried out waxy indentification by grain IKI, screening retains the waxy and unseparated individual plant of performance, and its corresponding rough Some seeds that do not go out is carried out hot and humid artificial accelerated aging process, artificial accelerated aging processing method is dried 6 days through-18 DEG C of refrigerator freezings 12 days and 44 DEG C, baking oven by seed, again temperature 42 DEG C, artificial accelerated aging 22d is carried out in the climatic cabinate of humidity 88%, take out seed and carry out germination test, screening germination rate enters plantation of future generation higher than the individual plant of 78%.BC
1f
3generation-BC
1f
6dai Zhong, every step needing sowing, the flowering stage of its material plantation is all arranged in ear under two-line sterile line can educate phase temperature condition blooms, every need the step identified the sterile phase pollen sterility of material to be all arranged in the sterile phase temperature condition of two-line sterile line under ear and bloom, the procedure that the sterile conversion temperature of material is identified is carried out in climatic cabinate.
" special 6311S " has naked body proterties, premature senescence resistance, seed storage endurance, rice is yellow and be waxy, the glutinous two-line hybrid rice of Huang of naked body, premature senescence resistance, storage endurance can be bred as with it and the restorer assembly with same proterties, this hybrid rice high yield, premature senescence resistance, storage endurance, be easy to mechanized harvest, rice is yellow, rice soft glutinous, mouthfeel good, be rich in multiple nutrients material, this hybrid rice is than standard hybridization rice volume increase about 6%, shelf time extends about 8 months, and price is 3.5-4.5 times of general rice.
Embodiment 3:
1) select naked body rice varieties " light 159 " (A), premature senescence resistance rice varieties " No. 62, grand round-grained rice " (B), storage endurance rice varieties " two autumns are short No. 1 " (C), yellow glutinous rice variety " glutinous millet is glutinous " (D), make hybridization A/B and C/D, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent, plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ F
1-CD, maturing stage sowing must be reestablished diplomatic relations F
1for seed, with F
1-ABCDrepresent;
2) F is planted
1-ABCDseed, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony 1300 strain, carry out blade naked body character screening in the early tillering stage, the maturing stage carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 300 strain individual plant to gather in the crops seed (F
3-ABCD), every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation of future generation;
3) strain plantation 2) screen the F obtained
3-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 250 strain individual plant sowing (F of economical character, yield traits excellence
4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, screening retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation of future generation;
4) strain plantation 3) F of gained
4-ABCD, plant two-line sterile line " C815S " simultaneously, select the strain of Agronomic excellence flowering stage and therefrom select elite seedling 5 strain, do with " C815S " maternal, do to hybridize " C815S "/F
4-ABCD, plantation hybridization F
1in generation, is (with F
1-Srepresent), selfing sowing obtains F
2-S;
5) 4 are planted) F of gained
2-Scolony 900 strain, blade naked body character screening is carried out in the early tillering stage, maturing stage carries out premature senescence resistance screening, naked body premature senescence resistance individual plant 300 strain individual plant is selected to gather in the crops seed, every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation (F of future generation
3-S);
6) strain plantation 5) screen the F obtained
3-S, plant " C815S ", flowering stage is done maternal with " C815S ", backcross " C815S "/F simultaneously
3-S, must backcross BC
1f
1generation, plantation BC
1f
1in generation, selfing sowing obtains BC
1f
2, repeat step 5) and obtain BC
1f
3generation;
7) BC
1f
3generation-BC
1f
6in generation, presses pedigree method plantation, every Dai Jun carries out premature senescence resistance and storage endurance screening, upper menu strain is by strain plantation, therefrom select that plant cane is sturdy, (pollen is through microexamination for the sterile plant 50 of big panicle many grains per panicle again, completely sterile), cut shift-in climatic cabinate 23 degree regeneration after pocket, select pollen loose powder good, the individual plant sowing that ripening rate is high, at BC
1f
6select that population characteristics is stable not to be separated generation, sterility is stablized and namely the good strain sizing of Fertility al-teration is bred as the glutinous two-line sterile line of Huang " special 815S " with naked body, premature senescence resistance, storage endurance.
In above-mentioned Breeding Process, the evaluation and screening method of 4 kinds of specialized characters and step are: the screening of naked body proterties carries out the screening of blade naked body proterties in the early tillering stage by strain, listing mark has the rice strain of naked body proterties, premature senescence resistance screening selects rice straw sturdy in the maturing stage listing in rice strain, blade keeps the individual plant sowing of green not early ageing, gained seed numbering is dried, dry rear storage 20 days, every part of seed is got part and is carried out rough process, naked eyes select brown rice performance yellow and unseparated individual plant, its brown rice is carried out waxy indentification by grain IKI, screening retains the waxy and unseparated individual plant of performance, and its corresponding rough Some seeds that do not go out is carried out hot and humid artificial accelerated aging process, artificial accelerated aging processing method is dried 6 days through-18 DEG C of refrigerator freezings 12 days and 44 DEG C, baking oven by seed, again temperature 42 DEG C, artificial accelerated aging 22d is carried out in the climatic cabinate of humidity 88%, take out seed and carry out germination test, screening germination rate enters plantation of future generation higher than the individual plant of 78%.BC
1f
3generation-BC
1f
6dai Zhong, every step needing sowing, the flowering stage of its material plantation is all arranged in ear under two-line sterile line can educate phase temperature condition blooms, every need the step identified the sterile phase pollen sterility of material to be all arranged in the sterile phase temperature condition of two-line sterile line under ear and bloom, the procedure that the sterile conversion temperature of material is identified is carried out in climatic cabinate.
" special 815S " has naked body proterties, premature senescence resistance, seed storage endurance, rice is yellow and be waxy, naked body can be bred as with the restorer assembly with same proterties with it, premature senescence resistance, the glutinous two-line hybrid rice of Huang of storage endurance, this hybrid rice energy premature senescence resistance, storage endurance, output is high, than standard hybridization rice volume increase about 7%, be easy to mechanized harvest, rice is yellow, rice is soft, mouthfeel is good, be rich in multiple nutrients material, shelf time extends about 8 months, nutrient composition content is high, rice is yellow, rice is soft glutinous, mouthfeel is good, there is good tonic effect, price is 3.5-4.5 times of general rice.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (4)
1. there is a selection for specialized character two-line sterile line, it is characterized in that: concrete steps are as follows:
Select naked body rice varieties-A, premature senescence resistance rice varieties-B, storage endurance rice varieties-C, yellow glutinous rice rice varieties-D, make hybridization A/B and C/D, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent, plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ F
1-CD, maturing stage sowing must be reestablished diplomatic relations F
1for seed, with F
1-ABCDrepresent;
Plantation F
1-ABCDseed, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony 1100-1300 strain, carry out blade naked body character screening in the early tillering stage, the maturing stage carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 200-300 strain individual plant sowing F
3-ABCDevery part of seed carries out yellow seed coat, waxy indentification, screening performance yellow seed coat, waxy unseparated individual plant carry out storage endurance qualification, and storage endurance qualification adopts artificial accelerated aging processing method, after the process of screening artificial accelerated aging germination rate higher than 78% individual plant enter plantation of future generation;
Strain plantation 2) screen the F obtained
3-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 200-250 strain individual plant sowing F of economical character, yield traits excellence
4-ABCD, every part of seed carries out yellow seed coat qualification, and the unseparated individual plant of screening performance yellow seed coat carries out artificial accelerated aging, and screening germination rate enters plantation of future generation higher than the individual plant of 78%;
Strain plantation 3) screen the F obtained
4-ABCD, plant two-line sterile line S simultaneously, select the strain of Agronomic excellence flowering stage and therefrom select elite seedling 5 strain, do with two-line sterile line S maternal, make hybridization S/F
4-ABCD, plantation hybridization F
1in generation, with F
1-Srepresent, selfing sowing obtains F
2-S;
Plantation 4) screen the F obtained
2-Scolony 800-900 strain, blade naked body character screening is carried out in the early tillering stage, maturing stage carries out premature senescence resistance screening, naked body premature senescence resistance individual plant 200-300 strain individual plant is selected to gather in the crops seed, every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 78% individual plant enter plantation F of future generation
3-S;
Strain plantation 5) screen the F obtained
3-S, plant two-line sterile line S, flowering stage is done maternal with two-line sterile line S, backcross S/F simultaneously
3-S, must backcross BC
1f
1generation, plantation BC
1f
1in generation, selfing sowing obtains BC
1f
2, repeat step 5) and obtain BC
1f
3generation;
BC
1f
3generation-BC
1f
6in generation, presses pedigree method plantation, and every Dai Jun carries out premature senescence resistance and storage endurance screening, and upper menu strain is by strain plantation, therefrom select that plant cane is sturdy, the sterile plant 50 of big panicle many grains per panicle again, cut shift-in climatic cabinate 23 DEG C regeneration after pocket, selection pollen loose powder is good, the individual plant sowing that ripening rate is high, at BC
1f
6select that population characteristics is stable not to be separated generation, sterility is stablized and namely the good strain sizing of Fertility al-teration is bred as the glutinous two-line sterile line of Huang with naked body, premature senescence resistance, storage endurance;
Described naked body rice varieties is " light 159 ", and premature senescence resistance rice varieties is " No. 62, grand round-grained rice ", and storage endurance rice varieties is " two autumns are short No. 1 ", and yellow glutinous rice variety is " glutinous millet is glutinous ", and two-line sterile line S is " 2018S ", " 6311S " and " C815S ".
2. the selection with specialized character two-line sterile line according to claim 1, it is characterized in that: the evaluation and screening method of 4 kinds of specialized characters and step are: the screening of naked body proterties carries out the screening of blade naked body proterties in the early tillering stage by strain, listing mark has the rice strain of naked body proterties, premature senescence resistance screening selects rice straw sturdy in the maturing stage listing in rice strain, blade keeps the individual plant sowing of green not early ageing, gained seed numbering is dried, dry rear storage 20 days, every part of seed is got part and is carried out rough process, naked eyes select brown rice performance yellow and unseparated individual plant, its brown rice is carried out waxy indentification by grain IKI, screening retains the waxy and unseparated individual plant of performance, and its corresponding rough Some seeds that do not go out is carried out hot and humid artificial accelerated aging process, artificial accelerated aging processing method is: dried 6 days through-18 DEG C of refrigerator freezings 12 days and 44 DEG C, baking oven by seed, again temperature 42 DEG C, artificial accelerated aging 22d is carried out in the climatic cabinate of humidity 88%, take out seed and carry out germination test, screening germination rate enters plantation of future generation higher than the individual plant of 78%.
3. the selection with specialized character two-line sterile line according to claim 1, is characterized in that: BC
1f
3generation-BC
1f
6dai Zhong, every step needing sowing, the flowering stage of its material plantation is all arranged in ear under two-line sterile line can educate phase temperature condition blooms, every need the step identified the sterile phase pollen sterility of material to be all arranged in the sterile phase temperature condition of two-line sterile line under ear and bloom, the procedure that the sterile conversion temperature of material is identified is carried out in climatic cabinate.
4. the selection with specialized character two-line sterile line according to claim 1, it is characterized in that: the male sterile line of seed selection has naked body proterties, premature senescence resistance, seed storage endurance, rice is yellow and be waxy, is bred as the glutinous two-line hybrid rice of Huang of naked body, premature senescence resistance, storage endurance with it and the restorer assembly with same proterties.
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