CN104642099B - A kind of selection with specialized character rice restorer - Google Patents

A kind of selection with specialized character rice restorer Download PDF

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CN104642099B
CN104642099B CN201510108044.0A CN201510108044A CN104642099B CN 104642099 B CN104642099 B CN 104642099B CN 201510108044 A CN201510108044 A CN 201510108044A CN 104642099 B CN104642099 B CN 104642099B
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CN104642099A (en
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陈幼玉
章清杞
李晓燕
黄荣华
陈志伟
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WUHAN LONGFECUND AGRICULTURE DEVELOPMENT CO., LTD.
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Fujian Agriculture and Forestry University
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Abstract

The invention discloses a kind of selection with specialized character rice restorer, belong to Special Rices breeding technical field.The present invention selects 4 to be respectively provided with naked body, premature senescence resistance, storage endurance, the waxy Special rice kind of yellow seed coat is material, by hybridization, reestablish diplomatic relations and to segregating generation naked body, premature senescence resistance, storage endurance, yellow seed coat, waxy, economical character, the screening of yield traits etc., incubation has naked body, premature senescence resistance, the yellow Oryza sativa L. var. glutinosa Matsum. restorer of storage endurance characteristic, assemble with its sterile line of character as having and can be bred as naked body, premature senescence resistance, the glutinous hybrid paddy rice of Huang of storage endurance, this hybrid paddy rice energy premature senescence resistance, storage endurance, yield is high, it is prone to mechanized harvest, rice yellow, rice is soft, in good taste, rich in multiple nutrients material.

Description

A kind of selection with specialized character rice restorer
Technical field
Present invention relates particularly to a kind of selection with specialized character rice restorer, belong to Special rice breeding skill Art field.
Background technology
People by grain husk shell, blade in common cultivated rice without the type of hair, referred to as naked body rice.The rice varieties of the U.S. is for adapting to The needs of mechanization, major part is all naked body rice, because its meter of matter is preferable, also has the characteristics such as specular removal, at the conventional product of China Plant the example that in selection-breeding, existing success utilizes." naked body rice ", because whole body is without hair, disease pest is difficult to attachment and invades, and performance has stronger Resistant to diseases and insects, skin can be reduced when manual harvesting and threshing and itch and damage to skin, it is easy to mechanized harvest, add Man-hour free from dust, it is possible to reduce environmental pollution, so also known as " environmental protection Oryza sativa L. ".Rice premature refers to late growth stage of rice stem and leaf etc. During the physiological function at position occurs the phenomenon of decline, maturation too early, stem and leaf is withered and yellow, affects photosynthesis, causes yield to decline. Senilism is caused by the characteristic of environment and kind, and a key factor of restriction Indica Rice output increased is exactly paddy growth at present The senilism phenomenon in later stage, the grain yield loss caused because of rice premature every year with regard to one, Jiangxi province reaches 10-15 hundred million kilograms.? Rice maturity functional leaf often extends 1 day and carries out photosynthesis, can increase by the yield of about 1%.Solve rice premature problem Good method is exactly the rice varieties of selection-breeding premature senescence resistance.The shoddy change of rice during rice storage, germination percentage decline always puzzlement Production, accumulating and a difficult problem of operating division, thereby result in China's foodstuff preservation loss average out to 3.05%.Rice paddy seed exists Storage also causes huge economic loss because vigor declines, and also the growth shape of percentage of seedgermination and seedling can be affected Condition.Therefore, cultivate storage endurance new rice variety, have great importance for solving the problems such as rice Deterioration.Semen setariae is Being produced yellow rind by the anthocyanidin in peel with yellow, anthocyanidin is described as " the special effect element of life ", and Semen setariae is the richest Containing vitamin A and several amino acids, nutrient composition content is high.Oryza glutinosa is nutritious, in good taste, is to manufacture viscosity snack and various The primary raw material of sweets.
By in above 4 kinds of character polymerization Introduced into Rice restorer, can be bred as having naked body, premature senescence resistance, storage endurance characteristic Yellow Oryza sativa L. var. glutinosa Matsum. restorer, assembles the glutinous hybridization of Huang that can be bred as naked body, premature senescence resistance, storage endurance with its sterile line of character as having Rice, this hybrid paddy rice energy premature senescence resistance, storage endurance, yield is high, be prone to mechanized harvest, rice yellow, and rice is soft, in good taste, rich in Multiple nutrients material, has wide market prospect and Development volue, and its market price is higher than common rice 3.5-4.5 times, pushes away Extensive farming plants the income for improving vast kind of rice peasant, for improving the trophic level of broad masses of the people, improves health All there is certain meaning.
Summary of the invention
It is an object of the invention to provide a kind of selection with specialized character rice restorer, utilize the method energy Select the glutinous restorer of Huang of naked body, premature senescence resistance, storage endurance.
The present invention selects naked body rice varieties " light 158 " and " light 159 ", premature senescence resistance rice varieties " grand round-grained rice 62 ", storage endurance Rice varieties " cloud extensive 290 " and yellow glutinous rice variety " Semen setariae is glutinous " are material, by hybridization, reestablish diplomatic relations and to segregating generation naked body, Premature senescence resistance, storage endurance, yellow seed coat, waxy, the screening of economical character, yield traits etc., be bred as having naked body, premature senescence resistance, resistance to The yellow Oryza sativa L. var. glutinosa Matsum. restorer of storage characteristics.
Technical scheme is as follows:
1) naked body rice varieties " light 158 " or " light 159 " (A), premature senescence resistance rice varieties " grand round-grained rice 62 " (B), storage endurance are selected Rice varieties " cloud extensive 290 " (C), yellow glutinous rice variety " Semen setariae is glutinous " (D) (Chen great Zhou, indica type Semen setariae Oryza sativa L. var. glutinosa Matsum. Breeding for restoration lines and profit Use technology. Agriculture in Jiangxi journal, 1993,5 (1): 7-20), make hybridization A/B and C/D, F must be hybridized1In generation, respectively with F1-ABWith F1-CDRepresent, plantation hybridization F1In generation, make hybridization F in florescence1-AB/F1-CD, period of maturation sowing must be reestablished diplomatic relations F1For seed, with F1-ABCD Represent;
2) plantation F1-ABCDSeed, selfing sowing obtains F2-ABCD, plant F2-ABCD, colony's 900-1000 strain, enter in the early tillering stage Row blade naked body character screening, the period of maturation carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 200-250 strain individual plant to gather in the crops seed (F3-ABCD), every part of seed carries out yellow seed coat, waxy and storage endurance is identified, screening retain performance yellow seed coat, waxy regardless of From and artificial accelerated aging process after germination percentage high 75% individual plant enter the next generation plantation;
3) strain plantation 2) screen the F obtained3-ABCD, the period of maturation select premature senescence resistance strain and therefrom select economical character, The individual plant 200-250 strain individual plant sowing (F that yield traits is excellent4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification (because naked body and waxy character are Recessive genes control, when showing this character, gene isozygotys, will not separate again), retains performance The individual plant that yellow seed coat does not separates and after artificial accelerated aging process, germination percentage is high enters next generation's plantation;
4) strain plantation 3) F of gained4-ABCD, the period of maturation selects the strain of premature senescence resistance and therefrom selects economical character, yield The individual plant 180-200 strain individual plant sowing (F that character is excellent5-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, protects Stay performance yellow seed coat not separate and after artificial accelerated aging process, the germination percentage individual plant higher than 75% enters next generation's plantation;
5) strain plantation 4) F of gained5-ABCD, the period of maturation selects the strain of premature senescence resistance and therefrom selects economical character, yield The individual plant 150-180 strain individual plant sowing (F that character is excellent6-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, protects Stay performance yellow seed coat not separate and after artificial accelerated aging process, the germination percentage individual plant higher than 75% enters next generation's plantation;
6) strain plantation 5) F of gained6-ABCD, select florescence population characteristics not separate, pollen loose powder is good, agronomy The individual plant listing mark that character is excellent, and the restorative of individual plant of listing is measured, select restorative good, fringe head is big, solid The individual plant 5 strain sizing that rate is high is i.e. bred as the yellow Oryza sativa L. var. glutinosa Matsum. restorer with naked body, premature senescence resistance, storage endurance.
The evaluation and screening method and steps of above-mentioned 4 kinds of specialized characters is: the screening of naked body character is by strain in the early tillering stage Carrying out the screening of blade naked body character, listing mark has the rice strain of naked body character, and premature senescence resistance screening is to list in the period of maturation Rice strain selects the individual plant sowing that rice straw is sturdy, blade keeps green not senilism, gained seed numbering is dried, stores after drying 20 days, every part of seed took part and carries out rough process, and naked eyes select brown rice performance yellow and unseparated individual plant, by its brown rice by Grain IKI carries out waxy indentification, and screening retains that performance is waxy and unseparated individual plant, and by its corresponding do not go out rough Some seeds carries out storage endurance qualification, and storage endurance is accredited as seed through-18 DEG C of refrigerator freezings 12 days and 44 DEG C of baking oven Dry 6 days, then temperature 42 DEG C, humidity 88% growth cabinet in carry out artificial accelerated aging 22d, take out seed and germinate Test, screening germination percentage is higher than 75%, shows yellow seed coat, the entrance next generation's plantation of waxy unseparated individual plant..
The beneficial effects of the present invention is: utilize the method can be respectively naked body character, premature senescence resistance, storage endurance, yellow kind Skin, waxy character import in restorer, and the restorer selected has naked body character, and premature senescence resistance, seed storage endurance, rice is waxy And seed coat is yellow, assemble the glutinous hybridization of Huang that can be bred as naked body, premature senescence resistance, storage endurance with its sterile line of character as having Rice, this hybrid paddy rice energy premature senescence resistance, storage endurance, yield is high, be prone to mechanized harvest, rice yellow, and rice is soft, in good taste, rich in Multiple nutrients material.This hybrid paddy rice increases production 5-7% than standard hybridization rice, and the storage phase extends 7-8 month, and price is general rice 3.5-4.5 times.
Detailed description of the invention:
Embodiment 1:
1) naked body rice varieties " light 158 " (A), premature senescence resistance rice varieties " grand round-grained rice 62 " (B), storage endurance rice varieties are selected " cloud extensive 290 " (C), yellow glutinous rice variety " Semen setariae is glutinous " (D), make hybridization A/B and C/D, must hybridize F1In generation, respectively with F1-ABAnd F1-CD Represent, plantation hybridization F1In generation, make hybridization F in florescence1-AB/F1-CD, period of maturation sowing must be reestablished diplomatic relations F1For seed, with F1-ABCDTable Show;
2) plantation F1-ABCDSeed, selfing sowing obtains F2-ABCD, plant F2-ABCD, colony 900 strain, carry out leaf in the early tillering stage Sheet naked body character screening, the period of maturation carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 200 strain individual plant to gather in the crops seed (F3-ABCD), Every part of seed carries out yellow seed coat, waxy and storage endurance is identified, screening has yellow seed coat, waxy do not separates and artificial acceleration After burin-in process, the germination percentage individual plant higher than 75% enters next generation's plantation;
3) strain plantation 2) screen the F obtained3-ABCD, the period of maturation select premature senescence resistance strain and therefrom select economical character, The individual plant 200 strain individual plant sowing (F that yield traits is excellent4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, protects Stay performance yellow seed coat not separate and after artificial accelerated aging process, the germination percentage individual plant higher than 75% enters next generation's plantation;
4) strain plantation 3) F of gained4-ABCD, the period of maturation selects the strain of premature senescence resistance and therefrom selects economical character, yield The individual plant 180 strain individual plant sowing (F that character is excellent5-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, reservation table The individual plant that existing yellow seed coat does not separates and after artificial accelerated aging process, germination percentage is high enters next generation's plantation;
5) strain plantation 4) F of gained5-ABCD, the period of maturation selects the strain of premature senescence resistance and therefrom selects economical character, yield The individual plant 150 strain individual plant sowing (F that character is excellent6-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, reservation table Existing yellow seed coat does not separates and after artificial accelerated aging process, the germination percentage individual plant higher than 75% enters next generation's plantation;
6) strain plantation 5) F of gained6-ABCD, select florescence population characteristics not separate, pollen loose powder is good, agronomy The individual plant listing mark that character is excellent, and the restorative of individual plant of listing is measured, select restorative good, fringe head is big, solid The individual plant 5 strain sizing that rate is high is i.e. bred as the yellow Oryza sativa L. var. glutinosa Matsum. restorer " special extensive No. 1 " with naked body, premature senescence resistance, storage endurance.
The evaluation and screening method and steps of above-mentioned 4 kinds of specialized characters is: the screening of naked body character is by strain in the early tillering stage Carrying out the screening of blade naked body character, listing mark has the rice strain of naked body character, and premature senescence resistance screening is to list in the period of maturation Rice strain selects the individual plant sowing that rice straw is sturdy, blade keeps green not senilism, gained seed numbering is dried, stores after drying 20 days, every part of seed took part and carries out rough process, and naked eyes select brown rice performance yellow and unseparated individual plant, by its brown rice by Grain IKI carries out waxy indentification, and screening retains that performance is waxy and unseparated individual plant, and by its corresponding do not go out rough Some seeds carries out storage endurance qualification, and storage endurance is accredited as seed through-18 DEG C of refrigerator freezings 12 days and 44 DEG C of baking oven Dry 6 days, then temperature 42 DEG C, humidity 88% growth cabinet in carry out artificial accelerated aging 22d, take out seed and germinate Test, screening germination percentage is higher than 75%, shows yellow seed coat, the entrance next generation's plantation of waxy unseparated individual plant.
The restorer " special extensive No. 1 " of the selection-breeding of the inventive method has naked body character, premature senescence resistance, seed storage endurance, rice Waxy and seed coat is yellow, assemble with its sterile line of character as having can be bred as naked body, premature senescence resistance, storage endurance Huang glutinous Hybrid paddy rice, this hybrid paddy rice energy premature senescence resistance, storage endurance, yield is high, be prone to mechanized harvest, rice yellow, and rice is soft, in good taste, Rich in multiple nutrients material.This hybrid paddy rice increases production about 5% than standard hybridization rice, and the storage phase extends 7 months, and price is common 3.5-4.5 times of rice.
Embodiment 2:
1) naked body rice varieties " light 159 " (A), premature senescence resistance rice varieties " grand round-grained rice 62 " (B), storage endurance rice varieties are selected " cloud extensive 290 " (C), yellow glutinous rice variety " Semen setariae is glutinous " (D), make hybridization A/B and C/D, must hybridize F1In generation, respectively with F1-ABAnd F1-CD Represent, plantation hybridization F1In generation, make hybridization F in florescence1-AB/F1-CD, period of maturation sowing must be reestablished diplomatic relations F1For seed, with F1-ABCDTable Show;
2) plantation F1-ABCDSeed, selfing sowing obtains F2-ABCD, plant F2-ABCD, colony 1000 strain, carry out leaf in the early tillering stage Sheet naked body character screening, the period of maturation carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 250 strain individual plant to gather in the crops seed (F3-ABCD), Every part of seed carries out yellow seed coat, waxy and storage endurance is identified, screening has yellow seed coat, waxy do not separates and artificial acceleration After burin-in process, the germination percentage individual plant higher than 75% enters next generation's plantation;
3) strain plantation 2) screen the F obtained3-ABCD, the period of maturation select premature senescence resistance strain and therefrom select economical character, The individual plant 250 strain individual plant sowing (F that yield traits is excellent4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, protects The individual plant that performance yellow seed coat does not separates and after artificial accelerated aging process, germination percentage is high is stayed to enter next generation's plantation;
4) strain plantation 3) F of gained4-ABCD, the period of maturation selects the strain of premature senescence resistance and therefrom selects economical character, yield The individual plant 200 strain individual plant sowing (F that character is excellent5-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, reservation table Existing yellow seed coat does not separates and after artificial accelerated aging process, the germination percentage individual plant higher than 75% enters next generation's plantation;
5) strain plantation 4) F of gained5-ABCD, the period of maturation selects the strain of premature senescence resistance and therefrom selects economical character, yield The individual plant 180 strain individual plant sowing (F that character is excellent6-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, reservation table Existing yellow seed coat does not separates and after artificial accelerated aging process, the germination percentage individual plant higher than 75% enters next generation's plantation;
6) strain plantation 5) F of gained6-ABCD, select florescence population characteristics not separate, pollen loose powder is good, agronomy The individual plant listing mark that character is excellent, and the restorative of individual plant of listing is measured, select restorative good, fringe head is big, solid The individual plant 5 strain sizing that rate is high is i.e. bred as the yellow Oryza sativa L. var. glutinosa Matsum. restorer " special extensive No. 2 " with naked body, premature senescence resistance, storage endurance.
The evaluation and screening method and steps of above-mentioned 4 kinds of specialized characters is: the screening of naked body character is by strain in the early tillering stage Carrying out the screening of blade naked body character, listing mark has the rice strain of naked body character, and premature senescence resistance screening is to list in the period of maturation Rice strain selects the individual plant sowing that rice straw is sturdy, blade keeps green not senilism, gained seed numbering is dried, stores after drying 20 days, every part of seed took part and carries out rough process, and naked eyes select brown rice performance yellow and unseparated individual plant, by its brown rice by Grain IKI carries out waxy indentification, and screening retains that performance is waxy and unseparated individual plant, and by its corresponding do not go out rough Some seeds carries out storage endurance qualification, and storage endurance is accredited as seed through-18 DEG C of refrigerator freezings 12 days and 44 DEG C of baking oven Dry 6 days, then temperature 42 DEG C, humidity 88% growth cabinet in carry out artificial accelerated aging 22d, take out seed and germinate Test, screening germination percentage is higher than 75%, shows yellow seed coat, the entrance next generation's plantation of waxy unseparated individual plant.
The restorer " special extensive No. 2 " that the inventive method selects has naked body character, premature senescence resistance, seed storage endurance, rice Waxy and seed coat is yellow, assemble with its sterile line of character as having can be bred as naked body, premature senescence resistance, storage endurance Huang glutinous Hybrid paddy rice, this hybrid paddy rice energy premature senescence resistance, storage endurance, yield is high, be prone to mechanized harvest, rice yellow, and rice is soft, in good taste, Rich in multiple nutrients material.This hybrid paddy rice increases production about 7% than standard hybridization rice, and the storage phase extends 8 months, and price is common 3.5-4.5 times of rice.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with Modify, all should belong to the covering scope of the present invention.

Claims (2)

1. a selection with specialized character rice restorer, it is characterised in that: specifically comprise the following steps that
Select naked body rice varieties-A, premature senescence resistance rice varieties-B, storage endurance rice varieties-C, yellow Oryza glutinosa rice varieties-D, make hybridization A/B And C/D, F must be hybridized1In generation, respectively with F1-ABAnd F1-CDRepresent, plantation hybridization F1In generation, make hybridization F in florescence1-AB/F1-CD, ripe Phase sowing must be reestablished diplomatic relations F1For seed, with F1-ABCDRepresent;
Plantation F1-ABCDSeed, selfing sowing obtains F2-ABCD, plant F2-ABCD, colony's 900-1000 strain, carry out blade in the early tillering stage Naked body character screening, the period of maturation carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 200-250 strain individual plant sowing F3-ABCD, every part Seed carries out yellow seed coat, waxy and storage endurance is identified, screening retains performance yellow seed coat, waxy do not separates and artificial acceleration After burin-in process, the germination percentage individual plant higher than 75% enters next generation's plantation;
Strain plantation 2) screen the F obtained3-ABCD, the period of maturation selects the strain of premature senescence resistance and therefrom selects economical character, yield Individual plant 200-250 strain individual plant sowing F that shape is excellent4-ABCD, every part of seed carries out yellow seed coat and storage endurance qualification, and screening is protected Stay performance yellow seed coat not separate and after artificial accelerated aging process, the germination percentage individual plant higher than 75% enters next generation's plantation;
Strain plantation 3) screen the F obtained4-ABCD, the period of maturation selects the strain of premature senescence resistance and therefrom selects economical character, yield Individual plant 180-200 strain individual plant sowing F that shape is excellent5-ABCD, every part of seed carries out yellow seed coat and storage endurance qualification, and screening is protected The individual plant that performance yellow seed coat does not separates and after artificial accelerated aging process, germination percentage is high is stayed to enter next generation's plantation;
Strain plantation 4) screen the F obtained5-ABCD, the period of maturation selects the strain of premature senescence resistance and therefrom selects economical character, yield Individual plant 150-180 strain individual plant sowing F that shape is excellent6-ABCD, every part of seed carries out yellow seed coat and storage endurance qualification, and screening is protected Stay performance yellow seed coat not separate and after artificial accelerated aging process, the germination percentage individual plant higher than 75% enters next generation's plantation;
Strain plantation 5) screen the F obtained6-ABCD, select florescence population characteristics not separate, pollen loose powder is good, agronomy The individual plant listing mark that shape is excellent, and the restorative of individual plant of listing is measured, select restorative good, fringe head big, setting percentage High individual plant 5 strain sizing is i.e. bred as the yellow Oryza sativa L. var. glutinosa Matsum. restorer with naked body, premature senescence resistance, storage endurance;
Described naked body rice varieties is " light 158 " or " light 159 ", and premature senescence resistance rice varieties is " grand round-grained rice 62 ", storage endurance Oryza sativa L. Kind is " cloud extensive 290 ", and yellow glutinous rice variety is " Semen setariae is glutinous ";
The evaluation and screening method and steps of 4 kinds of specialized characters is: the screening of naked body character is to carry out blade in the early tillering stage by strain The screening of naked body character, listing mark has the rice strain of naked body character, and premature senescence resistance screening is to select in rice strain of listing in the period of maturation Select the individual plant sowing that rice straw is sturdy, blade keeps green not senilism, gained seed numbering is dried, storage 20 days after drying, often Part seed takes part and carries out rough process, and naked eyes select brown rice performance yellow and unseparated individual plant, by its brown rice by grain with iodo- Potassium iodide carries out waxy indentification, and screening retains that performance is waxy and unseparated individual plant, and corresponding it is not gone out rough Some Species Son carries out storage endurance qualification, and storage endurance authentication method is that through-18 DEG C of refrigerator freezings 12 days and 44 DEG C of baking oven, seed is dried 6 My god, then temperature 42 DEG C, humidity 88% growth cabinet in carry out artificial accelerated aging 22d, take out seed and carry out examination of germinateing Testing, the screening germination percentage individual plant higher than 75% enters next generation's plantation.
The selection with specialized character rice restorer the most according to claim 1, it is characterised in that: selection-breeding extensive Multiple cording has naked body character, premature senescence resistance, seed storage endurance, rice waxy and seed coat is yellow, by its character as having Sterile line assembles incubation naked body, premature senescence resistance, the glutinous hybrid paddy rice of Huang of storage endurance.
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