CN104642099A - Breeding method of rice restoring line with special characters - Google Patents
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Abstract
The invention discloses a breeding method of a rice storing line with special characters, and belongs to the technical field of special rice breeding. The method comprises the following steps: selecting four special rice varieties respectively with glabrous property, premature senility resistance, endurable storage and yellow seed coat glutinous property, hybridizing and multi-crossing, screening the strains with glabrous property, premature senility resistance, endurable storage, yellow seed coat, glutinous property, agronomic character and yield character in segregative generation, breeding the yellow glutinous rice restoring line with the strains with glabrous property, premature senility resistance and endurable storage, assembling the yellow glutinous rice restoring line with the sterility line with the same characters to breed the glabrous, anti-premature senility and storage-endurable yellow glutinous hybrid rice. The hybrid rice is premature senility resistant, storage endurable, high in yield, easy for mechanically harvesting, the rice paddy is yellow, the rice is soft and good in taste and rich in various nutrients.
Description
Technical field
The present invention is specifically related to a kind of selection with specialized character rice restorer, belongs to Special rice breeding technical field.
Background technology
Shell clever in common cultivated rice, blade without the type of hair, are called naked body rice by people.The rice varieties of the U.S. is the needs adapting to mechanization, and major part is all naked body rice, because its meter of matter is better, also has the characteristics such as specular removal, has the example successfully utilized in the conventional variety seed selection of China." naked body rice ", because whole body is without hair, sick worm not easily adheres to and invades, and performance has stronger resistant to diseases and insects, skin can be reduced itch and damage to skin when manual harvesting and threshing, be easy to mechanized harvest, add free from dust in man-hour, environmental pollution can be reduced, so also known as " environmental protection paddy rice ".Rice premature refers to that the phenomenon failed appears in the physiological function at the positions such as late growth stage of rice cauline leaf too early, and ripening period cauline leaf is withered and yellow, affects photosynthesis, causes production declining.Early ageing is caused by the characteristic of environment and kind, and a key factor of restriction Indica Rice output increased is exactly the early ageing phenomenon of late growth stage of rice at present, and the grain yield loss caused because of rice premature every year with regard to one, Jiangxi province reaches 10-15 hundred million kilograms.Often extend 1 day at rice maturity functional leaf and carry out photosynthesis, can increase by the output of about 1%.Solve the rice varieties that rice premature problem the best way is exactly seed selection premature senescence resistance.It is that puzzlement is produced, a difficult problem of accumulating and operating division that the shoddy change of rice in rice storage process, germination rate decline always, causes China's foodstuff preservation loss average out to 3.05% thus.Rice paddy seed also causes huge economic loss because vigor declines in storage, and can affect the upgrowth situation of percentage of seedgermination and seedling.Therefore, cultivate storage endurance new rice variety, the problems such as solution rice Deterioration are had great importance.Glutinous millet produces yellow rind by having yellow anthocyan in pericarp, and anthocyan is described as " the special effect element of life ", and glutinous millet is also rich in vitamin A and several amino acids, and nutrient composition content is high.Glutinous rice is nutritious, and mouthfeel is good, is the primary raw material manufacturing viscosity snack and various sweets.
By in above 4 kinds of proterties polymerization Introduced into Rice restorer, can be bred as and there is naked body, premature senescence resistance, the yellow glutinous rice restorer of storage endurance characteristic, naked body can be bred as with the male sterile line assembly with same proterties with it, premature senescence resistance, the glutinous hybrid rice of Huang of storage endurance, this hybrid rice energy premature senescence resistance, storage endurance, output is high, be easy to mechanized harvest, rice is yellow, rice is soft, mouthfeel is good, be rich in multiple nutrients material, there is wide market prospects and Development volue, its market price than common rice height 3.5-4.5 doubly, popularizing planting is for the income improving vast kind of rice peasant, for the trophic level improving broad masses of the people, improve health and all there is certain meaning.
Summary of the invention
The object of the present invention is to provide a kind of selection with specialized character rice restorer, utilize the method can select the glutinous restorer of Huang of naked body, premature senescence resistance, storage endurance.
The present invention selects naked body rice varieties " light 158 " and " light 159 ", premature senescence resistance rice varieties " No. 62, grand round-grained rice ", storage endurance rice varieties " cloud extensive 290 " and yellow glutinous rice variety " glutinous millet is glutinous " to be material, by hybridization, reestablish diplomatic relations and screening to segregative generation naked body, premature senescence resistance, storage endurance, yellow seed coat, waxy, economical character, yield traits etc., be bred as the yellow glutinous rice restorer with naked body, premature senescence resistance, storage endurance characteristic.
Technical scheme of the present invention is as follows:
1) naked body rice varieties " light 158 " or " light 159 " (A), premature senescence resistance rice varieties " No. 62, grand round-grained rice " (B), storage endurance rice varieties " cloud extensive 290 " (C), yellow glutinous rice variety " glutinous millet is glutinous " (D) (Chen great Zhou is selected, indica type glutinous millet glutinous rice Breeding for restoration lines and utilize technology. Agriculture in Jiangxi journal, 1993,5 (1): 7-20), make hybridization A/B and C/D, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent, plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ F
1-CD, maturing stage sowing must be reestablished diplomatic relations F
1for seed, with F
1-ABCDrepresent;
2) F is planted
1-ABCDseed, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony 900-1000 strain, carry out blade naked body character screening in the early tillering stage, the maturing stage carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 200-250 strain individual plant to gather in the crops seed (F
3-ABCD), every part of seed carries out yellow seed coat, waxy and storage endurance qualification, and screening retains performance yellow seed coat, not waxyly to be separated and after artificial accelerated aging process, the individual plant of germination rate high 75% enters plantation of future generation;
3) strain plantation 2) screen the F obtained
3-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 200-250 strain individual plant sowing (F of economical character, yield traits excellence
4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification (because naked body and waxy proterties are that Recessive genes controls, when showing this proterties, gene isozygotys, can not be separated again), reservation performance yellow seed coat is not separated and the individual plant that after artificial accelerated aging process, germination rate is high enters plantation of future generation;
4) strain plantation 3) F of gained
4-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 180-200 strain individual plant sowing (F of economical character, yield traits excellence
5-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
5) strain plantation 4) F of gained
5-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 150-180 strain individual plant sowing (F of economical character, yield traits excellence
6-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
6) strain plantation 5) F of gained
6-ABCDselect flowering stage population characteristics to stablize not to be separated, pollen loose powder is good, the individual plant listing mark of economical character excellence, and the restorative of individual plant of listing is measured, select restorative good, that fringe head is large, ripening rate is high individual plant 5 strain sizing to be namely bred as the yellow glutinous rice restorer with naked body, premature senescence resistance, storage endurance.
Evaluation and screening method and the step of above-mentioned 4 kinds of specialized characters are: the screening of naked body proterties carries out the screening of blade naked body proterties in the early tillering stage by strain, listing mark has the rice strain of naked body proterties, premature senescence resistance screening selects rice straw sturdy in the maturing stage listing in rice strain, blade keeps the individual plant sowing of green not early ageing, gained seed numbering is dried, dry rear storage 20 days, every part of seed is got part and is carried out rough process, naked eyes select brown rice performance yellow and unseparated individual plant, its brown rice is carried out waxy indentification by grain IKI, screening retains the waxy and unseparated individual plant of performance, and its corresponding rough Some seeds that do not go out is carried out storage endurance qualification, storage endurance is accredited as and is dried 6 days through-18 DEG C of refrigerator freezings 12 days and 44 DEG C, baking oven by seed, again temperature 42 DEG C, artificial accelerated aging 22d is carried out in the climatic cabinate of humidity 88%, take out seed and carry out germination test, screening germination rate is higher than 75%, performance yellow seed coat, waxy unseparated individual plant enters plantation of future generation.。
Beneficial effect of the present invention is: utilize the method respectively naked body proterties, premature senescence resistance, storage endurance, yellow seed coat, waxy proterties to be imported in restorer, the restorer selected has naked body proterties, premature senescence resistance, seed storage endurance, rice is waxy and seed coat is yellow, the glutinous hybrid rice of Huang of naked body, premature senescence resistance, storage endurance can be bred as with it and the male sterile line assembly with same proterties, this hybrid rice energy premature senescence resistance, storage endurance, output is high, be easy to mechanized harvest, rice is yellow, rice is soft, mouthfeel good, is rich in multiple nutrients material.This hybrid rice is than standard hybridization rice volume increase 5-7%, and shelf time extends 7-8 month, and price is 3.5-4.5 times of general rice.
embodiment:
Embodiment 1:
1) select naked body rice varieties " light 158 " (A), premature senescence resistance rice varieties " No. 62, grand round-grained rice " (B), storage endurance rice varieties " cloud extensive 290 " (C), yellow glutinous rice variety " glutinous millet is glutinous " (D), make hybridization A/B and C/D, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent, plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ F
1-CD, maturing stage sowing must be reestablished diplomatic relations F
1for seed, with F
1-ABCDrepresent;
2) F is planted
1-ABCDseed, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony 900 strain, carry out blade naked body character screening in the early tillering stage, the maturing stage carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 200 strain individual plant to gather in the crops seed (F
3-ABCD), every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening have yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
3) strain plantation 2) screen the F obtained
3-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 200 strain individual plant sowing (F of economical character, yield traits excellence
4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
4) strain plantation 3) F of gained
4-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 180 strain individual plant sowing (F of economical character, yield traits excellence
5-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, and reservation performance yellow seed coat is not separated and the individual plant that after artificial accelerated aging process, germination rate is high enters plantation of future generation;
5) strain plantation 4) F of gained
5-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 150 strain individual plant sowing (F of economical character, yield traits excellence
6-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
6) strain plantation 5) F of gained
6-ABCDselect flowering stage population characteristics to stablize not to be separated, pollen loose powder is good, the individual plant listing mark of economical character excellence, and the restorative of individual plant of listing is measured, select restorative good, that fringe head is large, ripening rate is high individual plant 5 strain sizing to be namely bred as the yellow glutinous rice restorer " special extensive No. 1 " with naked body, premature senescence resistance, storage endurance.
Evaluation and screening method and the step of above-mentioned 4 kinds of specialized characters are: the screening of naked body proterties carries out the screening of blade naked body proterties in the early tillering stage by strain, listing mark has the rice strain of naked body proterties, premature senescence resistance screening selects rice straw sturdy in the maturing stage listing in rice strain, blade keeps the individual plant sowing of green not early ageing, gained seed numbering is dried, dry rear storage 20 days, every part of seed is got part and is carried out rough process, naked eyes select brown rice performance yellow and unseparated individual plant, its brown rice is carried out waxy indentification by grain IKI, screening retains the waxy and unseparated individual plant of performance, and its corresponding rough Some seeds that do not go out is carried out storage endurance qualification, storage endurance is accredited as and is dried 6 days through-18 DEG C of refrigerator freezings 12 days and 44 DEG C, baking oven by seed, again temperature 42 DEG C, artificial accelerated aging 22d is carried out in the climatic cabinate of humidity 88%, take out seed and carry out germination test, screening germination rate is higher than 75%, performance yellow seed coat, waxy unseparated individual plant enters plantation of future generation.
The restorer " special extensive No. 1 " of the seed selection of the inventive method has naked body proterties, premature senescence resistance, seed storage endurance, rice is waxy and seed coat is yellow, the glutinous hybrid rice of Huang of naked body, premature senescence resistance, storage endurance can be bred as with it and the male sterile line assembly with same proterties, this hybrid rice energy premature senescence resistance, storage endurance, output is high, be easy to mechanized harvest, and rice is yellow, rice is soft, mouthfeel good, is rich in multiple nutrients material.This hybrid rice is than standard hybridization rice volume increase about 5%, and shelf time extends 7 months, and price is 3.5-4.5 times of general rice.
Embodiment 2:
1) select naked body rice varieties " light 159 " (A), premature senescence resistance rice varieties " No. 62, grand round-grained rice " (B), storage endurance rice varieties " cloud extensive 290 " (C), yellow glutinous rice variety " glutinous millet is glutinous " (D), make hybridization A/B and C/D, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent, plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ F
1-CD, maturing stage sowing must be reestablished diplomatic relations F
1for seed, with F
1-ABCDrepresent;
2) F is planted
1-ABCDseed, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony 1000 strain, carry out blade naked body character screening in the early tillering stage, the maturing stage carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 250 strain individual plant to gather in the crops seed (F
3-ABCD), every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening have yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
3) strain plantation 2) screen the F obtained
3-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 250 strain individual plant sowing (F of economical character, yield traits excellence
4-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, and reservation performance yellow seed coat is not separated and the individual plant that after artificial accelerated aging process, germination rate is high enters plantation of future generation;
4) strain plantation 3) F of gained
4-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 200 strain individual plant sowing (F of economical character, yield traits excellence
5-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
5) strain plantation 4) F of gained
5-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 180 strain individual plant sowing (F of economical character, yield traits excellence
6-ABCD), every part of seed carries out yellow seed coat and storage endurance qualification, retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
6) strain plantation 5) F of gained
6-ABCDselect flowering stage population characteristics to stablize not to be separated, pollen loose powder is good, the individual plant listing mark of economical character excellence, and the restorative of individual plant of listing is measured, select restorative good, that fringe head is large, ripening rate is high individual plant 5 strain sizing to be namely bred as the yellow glutinous rice restorer " special extensive No. 2 " with naked body, premature senescence resistance, storage endurance.
Evaluation and screening method and the step of above-mentioned 4 kinds of specialized characters are: the screening of naked body proterties carries out the screening of blade naked body proterties in the early tillering stage by strain, listing mark has the rice strain of naked body proterties, premature senescence resistance screening selects rice straw sturdy in the maturing stage listing in rice strain, blade keeps the individual plant sowing of green not early ageing, gained seed numbering is dried, dry rear storage 20 days, every part of seed is got part and is carried out rough process, naked eyes select brown rice performance yellow and unseparated individual plant, its brown rice is carried out waxy indentification by grain IKI, screening retains the waxy and unseparated individual plant of performance, and its corresponding rough Some seeds that do not go out is carried out storage endurance qualification, storage endurance is accredited as and is dried 6 days through-18 DEG C of refrigerator freezings 12 days and 44 DEG C, baking oven by seed, again temperature 42 DEG C, artificial accelerated aging 22d is carried out in the climatic cabinate of humidity 88%, take out seed and carry out germination test, screening germination rate is higher than 75%, performance yellow seed coat, waxy unseparated individual plant enters plantation of future generation.
The restorer " special extensive No. 2 " that the inventive method selects has naked body proterties, premature senescence resistance, seed storage endurance, rice is waxy and seed coat is yellow, the glutinous hybrid rice of Huang of naked body, premature senescence resistance, storage endurance can be bred as with it and the male sterile line assembly with same proterties, this hybrid rice energy premature senescence resistance, storage endurance, output is high, be easy to mechanized harvest, and rice is yellow, rice is soft, mouthfeel good, is rich in multiple nutrients material.This hybrid rice is than standard hybridization rice volume increase about 7%, and shelf time extends 8 months, and price is 3.5-4.5 times of general rice.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (3)
1. there is a selection for specialized character rice restorer, it is characterized in that: concrete steps are as follows:
Select naked body rice varieties-A, premature senescence resistance rice varieties-B, storage endurance rice varieties-C, yellow glutinous rice rice varieties-D, make hybridization A/B and C/D, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent, plantation hybridization F
1in generation, make hybridization F in flowering stage
1-AB/ F
1-CD, maturing stage sowing must be reestablished diplomatic relations F
1for seed, with F
1-ABCDrepresent;
Plantation F
1-ABCDseed, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony 900-1000 strain, carry out blade naked body character screening in the early tillering stage, the maturing stage carries out premature senescence resistance screening, selects naked body premature senescence resistance individual plant 200-250 strain individual plant sowing F
3-ABCD, every part of seed carries out yellow seed coat, waxy and storage endurance qualification, screening retain performance yellow seed coat, waxy be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
Strain plantation 2) screen the F obtained
3-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 200-250 strain individual plant sowing F of economical character, yield traits excellence
4-ABCD, every part of seed carries out yellow seed coat and storage endurance qualification, screening retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
Strain plantation 3) screen the F obtained
4-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 180-200 strain individual plant sowing F of economical character, yield traits excellence
5-ABCD, every part of seed carries out yellow seed coat and storage endurance qualification, and screening reservation performance yellow seed coat is not separated and the individual plant that after artificial accelerated aging process, germination rate is high enters plantation of future generation;
Strain plantation 4) screen the F obtained
5-ABCD, the maturing stage is selected the strain of premature senescence resistance and therefrom selects the individual plant 150-180 strain individual plant sowing F of economical character, yield traits excellence
6-ABCD, every part of seed carries out yellow seed coat and storage endurance qualification, screening retain performance yellow seed coat be not separated and after artificial accelerated aging process germination rate higher than 75% individual plant enter plantation of future generation;
Strain plantation 5) screen the F obtained
6-ABCDselect flowering stage population characteristics to stablize not to be separated, pollen loose powder is good, economical character is excellent individual plant listing mark, and the restorative of individual plant of listing is measured, select restorative good, that fringe head is large, ripening rate is high individual plant 5 strain sizing to be namely bred as the yellow glutinous rice restorer with naked body, premature senescence resistance, storage endurance;
Described naked body rice varieties is " light 158 " and " light 159 ", and premature senescence resistance rice varieties is " No. 62, grand round-grained rice ", and storage endurance rice varieties is " cloud extensive 290 ", and yellow glutinous rice variety is " glutinous millet is glutinous ".
2. the selection with specialized character rice restorer according to claim 1, it is characterized in that: the evaluation and screening method of 4 kinds of specialized characters and step are: the screening of naked body proterties carries out the screening of blade naked body proterties in the early tillering stage by strain, listing mark has the rice strain of naked body proterties, premature senescence resistance screening selects rice straw sturdy in the maturing stage listing in rice strain, blade keeps the individual plant sowing of green not early ageing, gained seed numbering is dried, dry rear storage 20 days, every part of seed is got part and is carried out rough process, naked eyes select brown rice performance yellow and unseparated individual plant, its brown rice is carried out waxy indentification by grain IKI, screening retains the waxy and unseparated individual plant of performance, and its corresponding rough Some seeds that do not go out is carried out storage endurance qualification, storage endurance authentication method is dried 6 days through-18 DEG C of refrigerator freezings 12 days and 44 DEG C, baking oven by seed, again temperature 42 DEG C, artificial accelerated aging 22d is carried out in the climatic cabinate of humidity 88%, take out seed and carry out germination test, screening germination rate enters plantation of future generation higher than the individual plant of 75%.
3. the selection with specialized character rice restorer according to claim 1, it is characterized in that: the restorer of seed selection has the waxy and seed coat of naked body proterties, premature senescence resistance, seed storage endurance, rice for yellow, is bred as the glutinous hybrid rice of Huang of naked body, premature senescence resistance, storage endurance with it and the male sterile line assembly with same proterties.
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| CN105941137A (en) * | 2016-07-07 | 2016-09-21 | 福建农林大学 | Breeding method of broad-resistance green soybean variety |
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