CN102524052A - Breeding method for producing paddy rice with colorful rice producing function - Google Patents
Breeding method for producing paddy rice with colorful rice producing function Download PDFInfo
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- CN102524052A CN102524052A CN2012100066103A CN201210006610A CN102524052A CN 102524052 A CN102524052 A CN 102524052A CN 2012100066103 A CN2012100066103 A CN 2012100066103A CN 201210006610 A CN201210006610 A CN 201210006610A CN 102524052 A CN102524052 A CN 102524052A
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Abstract
The invention particularly relates to a breeding method for producing paddy rice with a colorful rice producing function and belongs to the field of special rice breeding. The breeding method comprises the following steps of: (1) selecting a three-line maintenance line B as a female parent to be hybridized with a black rice male parent, carrying out multi-generation backcrossing on the first filial generation and the maintenance line B, and breeding a maintenance line black B with black seed coats and with other properties the same as the maintenance line B; (2) using a sterile line A corresponding to the three-line maintenance line B to be hybridized with the maintenance line black B to produce a sterile line black A containing heterozygous black seed coat genes; (3) using the sterile line black A to be hybridized with a restore line endosperm R to produce first filial F1 generation seeds filial F1; and (4) using the first filial F1 generation seeds filial F1 as production seeds. The breeding method has the advantages that hybridized paddy rice seeds capable of being directly used for producing colorful rice can be produced through the breeding method, and the functional colorful rice can be produced through planting the seeds. The production method is simple, the efficiency is high, the benefits are good, and the produced rice is rich in various nutrition elements.
Description
Technical field
The present invention is specifically related to a kind of selection that produces colored Mi Gongneng rice that has, and belongs to special rice breeding field.
Background technology
In recent years, the seed selection that has special proterties rice has received various countries breeding work person's attention.There is abundant extraordinary rice germplasm resource in China, and like black rice rice, purple rice rice, glutinous rice, giant embryo rice, fragrant rice etc., the nutritive value of these special rices is very high, and its some contained functional components have unique effect to the health care of human body.Contain abundant GABA like giant embryo rice, black rice contains anthocyan, carotin and cardiac glycoside, integrates color and nutrition curative effect.More existing dissimilar rice mix on the domestic and international market at present " five colours rice " is sold; Its preparation method is the separately plantation production of dissimilar rice, husk rice; Mechanical mixture forms in proportion again, and this kind method operation is quite loaded down with trivial details, and production cost is high.
Summary of the invention
The object of the present invention is to provide a kind of selection that produces colored Mi Gongneng rice that has; Utilize genetic principle and cross breeding method, as long as the preparation of seed selection efficiently " colored rice " hybrid rice seed reaches a kind of hybrid rice seed of plantation; The paddy of its harvest can contain the purpose of polytype and color rice simultaneously; Not only can produce the functional rice of high nutrition, can also simplify the production process of " colored rice ", reduce production costs.
Principle of the present invention is: rice glutinous trait proterties, giant embryo proterties are controlled by single recessive gene, with common rice hybridization F
2Generation glutinous property (giant embryo): non-glutinous property (non-giant embryo) is 1:3 and separates; Paddy rice black race skin proterties is mainly by a pair of dominant gene control, with common rice hybridization F
2For black rice: rice is 3:1 and separates.At the hybridization generation kind period of the day from 11 p.m. to 1 a.m, the paddy rice through seed selection tool various trait characteristic does the father and mother parent respectively, and it hybridizes F
1Paddy (the F of seed plantation back results
2), because of the separation of these proterties produces dissimilar and rice different colours.
The technical scheme of taking of the present invention is following:
A kind of selection that produces colored Mi Gongneng rice that has of the present invention may further comprise the steps:
(1) select three to be that maintenance line B makes maternal and black rice rice paternal hybrid, the first generation of hybrid and maintenance line B are many, and in generation, backcross, and seed selection contains black race's skin and other proterties maintenance line black B consistent with maintenance line B;
(2) use and three be that the black A of male sterile line is produced in the black B hybridization of the corresponding male sterile line A of maintenance line B and maintenance line, contain heterozygosis black race scytoblastema because of;
(3) be that hybrid F is produced in the glutinous R hybridization of giant embryo with black A of male sterile line and recovery
1For seed " assorted F
1", contain heterozygosis black race skin, giant embryo and glutinous gene simultaneously;
(4) hybrid F
1For seed " assorted F
1" promptly can be used as and produce with planting.
Said recovery is that the selection of the glutinous R of giant embryo is: with giant embryo rice and glutinous rice hybridization, F
2The brown rice that the generation selection has giant embryo and glutinous proterties simultaneously is through F
3Generation-F
6Select for pedigree, selecting the glutinous recovery of giant embryo is the glutinous R of giant embryo, contains giant embryo and glutinous homozygous gene.
Said three is that maintenance line B is golden 23B or Long Tepu B.
Said hybrid F
1For seed " assorted F
1", its paddy hybrid F that gathers in the crops of plantation back
2In generation, produce the rice of the dissimilar and color of 8 kinds of common rice, black rice, glutinous rice, giant embryo rice, black glutinous rice, giant embryo glutinous rice, giant embryo black rice and giant embryo black glutinous rices owing to heterozygosis black race skin, giant embryo and glutinous Gene Isolation.
Concrete steps are following:
A. three is maintenance line B
/Black rice rice → maintenance line B
/F
1→ with maintenance line B as recurrent parent (female parent); Selection contains black race's skin and other proterties individual plant more consistent with former B done the male parent pollination; In many generations, backcross to breed and contain black race's skin and other proterties and " deceive B " with former B system consistent black rice maintenance line (genotype is: CCWxWxGeGe); " black B " contains black race's skin dominant gene (represent black race's skin dominant gene with CC, represent the equipotential white race skin recessive gene that it is corresponding with cc).
B. doing three is that (genotype is: ccWxWxGeGe) with (CCWxWxGeGe) hybridization of black rice maintenance line " black B ": (genotype is A the corresponding male sterile line A of maintenance line B: ccWxWxGeGe)
/(genotype is " black B " → black rice male sterile line " black A ": CcWxWxGeGe) (contain heterozygosis black race scytoblastema because of Cc; Wherein: wx-glutinous trait gene, Wx-non-glutinous trait gene, ge-giant embryo gene, Ge-non-giant embryo gene).
C. with giant embryo rice and glutinous rice hybridization, F
2In generation, go out the brown rice that rough selection has giant embryo and glutinous proterties simultaneously, through F
3Generation-F
6Becoming the glutinous recovery of giant embryo for seed selection is " the glutinous R of giant embryo " (genotype is: ccwxwxgege contains giant embryo and glutinous proterties).
D. " deceive A " (CcWxWxGeGe)
/" the glutinous R of giant embryo " (ccwxwxgege) → produce with kind an of " assorted F
1" (genotype is: CcWxwxGege and ccWxwxGege).
E. " F mixes
1" paddy (F of (CcWxwxGege and ccWxwxGege) field planting → results
2Generation); Have 27 kinds of genotype, comprising: ccWxWxGeGe, CcWxWxGeGe, CCWxWxGeGe, ccWxwxGeGe, CcWxwxGeGe, CCWxwxGeGe, ccwxwxGeGe, CcwxwxGeGe, CCwxwxGeGe, ccWxWxGege, CcWxWxGege, CCWxWxGege, ccWxwxGege, CcWxwxGege, CCWxwxGege, ccwxwxGege, CcwxwxGege, CCwxwxGege, ccWxWxgege, CcWxWxgege, CCWxWxgege, ccWxwxgege, CcWxwxgege, CCWxwxgege, ccwxwxgege, Ccwxwxgege, CCwxwxgege.More than 27 kinds of genotypic phenotypes have: common rice, black rice, glutinous rice, giant embryo rice, black glutinous rice, giant embryo glutinous rice, giant embryo black rice and 8 kinds of giant embryo black glutinous rices are dissimilar.That color has is pitch black, somber, milky white, transparent white etc.
Effect of the present invention is that the heterozygous genes polymerization of the black race of handle control simultaneously skin, giant embryo, three kinds of proterties of glutinous property is in hybrid F
1, its paddy of gathering in the crops of plantation back produces the rice of all kinds combination because of the separation and the reorganization of these genes in the production.Advantage of the present invention is the hybrid rice seed that can directly produce " colored rice " through breeding method, plants this seed and can produce functional form " colored rice ".Production method is simple, efficient is high, profitable, the rice of production is rich in various nutritive elements.
Embodiment:
Below be the example of practical implementation of the present invention, further describe the present invention, but the present invention is not limited only to this.
Embodiment one:
A. golden 23B
/No. 2 → golden 23B of Qin rice
/F
1→ with golden 23B as recurrent parent (female parent), select to contain black race's skin and other proterties individual plant more consistent with golden 23B pollinated, how to contain black race's skin and other proterties maintenance line " dark fund 23B " consistent with golden 23B for backcrossing to breed.
B. golden 23 A
/" dark fund 23B " → " dark fund 23A ".
C. the glutinous No. 6 → F of giant embryo rice MhgeR/ chaste tree
1→ F
2In generation, go out brown rice → F that rough selection has giant embryo and glutinous proterties simultaneously
3Generation-F
6Select seed selection to become the glutinous recovery system of giant embryo " the glutinous R of giant embryo " for pedigree.
D. " dark fund 23A "
/" the glutinous R of giant embryo " → produce with kind of " black huge glutinous No. 1 an of Jin You ".
E. field planting " Jin You black huge glutinous No. 1 " → results " colored rice " paddy; Go out rough; " colored rice ", contain the rice of the dissimilar and color of 8 kinds of common rice, black rice, glutinous rice, giant embryo rice, black glutinous rice, giant embryo glutinous rice, giant embryo black rice and giant embryo black glutinous rices.
Embodiment two:
A. Long Te Pu B
/No. 2 → Long Tepu of Qin rice B
/F
1→ with Long Tepu B as recurrent parent (female parent), select to contain black race's skin and other proterties individual plant more consistent with Long Tepu B pollinated, how to contain black race's skin and other proterties maintenance line " Hei Longtepu B " consistent with Long Tepu B for backcrossing to breed.
B. Long Te Pu A
/" Hei Longtepu B " → " Hei Longtepu A ".
C. the glutinous No. 6 → F of giant embryo rice MhgeR/ chaste tree
1→ F
2In generation, go out brown rice → F that rough selection has giant embryo and glutinous proterties simultaneously
3Generation-F
6Become the glutinous recovery system of giant embryo " the glutinous R of giant embryo " for seed selection.
D. " Hei Longtepu A "
/" the glutinous R of giant embryo " → produce with kind of " special excellent black huge glutinous No. 1 ".
E. field planting " special excellent black huge glutinous No. 1 " → results " colored rice " paddy; Go out rough; " colored rice ", contain the rice of the dissimilar and color of 8 kinds of common rice, black rice, glutinous rice, giant embryo rice, black glutinous rice, giant embryo glutinous rice, giant embryo black rice and giant embryo black glutinous rices.
Claims (4)
1. one kind has the selection that produces colored Mi Gongneng rice, and it is characterized in that: said selection may further comprise the steps:
(1) select three to be that maintenance line B makes maternal and black rice rice paternal hybrid, the first generation of hybrid and maintenance line B are many, and in generation, backcross, and seed selection contains black race's skin and other proterties maintenance line black B consistent with maintenance line B;
(2) use and three be that the black A of male sterile line is produced in the black B hybridization of the corresponding male sterile line A of maintenance line B and maintenance line, contain heterozygosis black race scytoblastema because of;
(3) be that hybrid F is produced in the glutinous R hybridization of giant embryo with black A of male sterile line and recovery
1For seed " assorted F
1", contain heterozygosis black race skin, giant embryo and glutinous gene simultaneously;
(4) hybrid F
1For seed " assorted F
1" promptly can be used as and produce with planting.
2. the selection with the colored Mi Gongneng rice of product according to claim 1, it is characterized in that: said recovery is that the selection of the glutinous R of giant embryo is: with giant embryo rice and glutinous rice hybridization, F
2The brown rice that the generation selection has giant embryo and glutinous proterties simultaneously is through F
3Generation-F
6Select for pedigree, selecting the glutinous recovery of giant embryo is the glutinous R of giant embryo, contains giant embryo and glutinous homozygous gene.
3. the selection with the colored Mi Gongneng rice of product according to claim 1, it is characterized in that: said three is that maintenance line B is golden 23B or Long Tepu B.
4. the selection with the colored Mi Gongneng rice of product according to claim 1 is characterized in that: said hybrid F
1For seed " assorted F
1", its paddy hybrid F that gathers in the crops of plantation back
2In generation, produce the rice of the dissimilar and color of 8 kinds of common rice, black rice, glutinous rice, giant embryo rice, black glutinous rice, giant embryo glutinous rice, giant embryo black rice and giant embryo black glutinous rices owing to heterozygosis black race skin, giant embryo and glutinous Gene Isolation.
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|---|---|---|---|
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891605A (en) * | 2014-04-04 | 2014-07-02 | 福建农林大学 | Breeding method for disease-resistant functional rice two-line sterile line |
| CN104642099A (en) * | 2015-03-12 | 2015-05-27 | 福建农林大学 | Breeding method of rice restoring line with special characters |
| CN104823839A (en) * | 2015-05-22 | 2015-08-12 | 福建农林大学 | Breeding method of anthocyanin-rich black rice thermo-sensitive two-line sterile line |
| CN105981644A (en) * | 2015-10-28 | 2016-10-05 | 江西省农业科学院农产品质量安全与标准研究所 | Breeding method of rice new variety with pressure reducing function |
| CN106069729A (en) * | 2016-07-07 | 2016-11-09 | 福建农林大学 | A kind of selection of colorful wheat kind |
| CN107047285A (en) * | 2017-04-18 | 2017-08-18 | 福建省农业科学院水稻研究所 | A kind of selection for viewing and admiring colored rice varieties |
| CN108575727A (en) * | 2018-04-04 | 2018-09-28 | 中国水稻研究所 | A kind of breeding method of colour rice |
| CN111264381A (en) * | 2020-03-23 | 2020-06-12 | 浙江大学 | Breeding method of colored rice three-line hybrid combination |
| WO2021190130A1 (en) * | 2020-03-23 | 2021-09-30 | 浙江大学 | Method for selective breeding of colorful two-line hybrid rice |
| WO2021190139A1 (en) * | 2020-03-23 | 2021-09-30 | 浙江大学 | Method for breeding colored rice two-line hybrid rice |
| CN114145226A (en) * | 2021-07-22 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of sterile line Hongde A of three-line red rice of rice |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891605B (en) * | 2014-04-04 | 2015-06-03 | 福建农林大学 | Breeding method for disease-resistant functional rice two-line sterile line |
| CN103891605A (en) * | 2014-04-04 | 2014-07-02 | 福建农林大学 | Breeding method for disease-resistant functional rice two-line sterile line |
| CN104642099A (en) * | 2015-03-12 | 2015-05-27 | 福建农林大学 | Breeding method of rice restoring line with special characters |
| CN104823839A (en) * | 2015-05-22 | 2015-08-12 | 福建农林大学 | Breeding method of anthocyanin-rich black rice thermo-sensitive two-line sterile line |
| CN105981644A (en) * | 2015-10-28 | 2016-10-05 | 江西省农业科学院农产品质量安全与标准研究所 | Breeding method of rice new variety with pressure reducing function |
| CN106069729B (en) * | 2016-07-07 | 2018-10-23 | 江苏福多美生物科技有限公司 | A kind of selection of colorful wheat kind |
| CN106069729A (en) * | 2016-07-07 | 2016-11-09 | 福建农林大学 | A kind of selection of colorful wheat kind |
| CN107047285A (en) * | 2017-04-18 | 2017-08-18 | 福建省农业科学院水稻研究所 | A kind of selection for viewing and admiring colored rice varieties |
| CN107047285B (en) * | 2017-04-18 | 2020-08-25 | 福建省农业科学院水稻研究所 | Breeding method of ornamental colored rice variety |
| CN108575727A (en) * | 2018-04-04 | 2018-09-28 | 中国水稻研究所 | A kind of breeding method of colour rice |
| CN111264381A (en) * | 2020-03-23 | 2020-06-12 | 浙江大学 | Breeding method of colored rice three-line hybrid combination |
| WO2021190131A1 (en) * | 2020-03-23 | 2021-09-30 | 浙江大学 | Breeding method for three-line hybrid combination of colored rice |
| WO2021190130A1 (en) * | 2020-03-23 | 2021-09-30 | 浙江大学 | Method for selective breeding of colorful two-line hybrid rice |
| WO2021190139A1 (en) * | 2020-03-23 | 2021-09-30 | 浙江大学 | Method for breeding colored rice two-line hybrid rice |
| CN114145226A (en) * | 2021-07-22 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of sterile line Hongde A of three-line red rice of rice |
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