CN103404430A - Method for breeding high-pro-vitamin A content maize inbred lines - Google Patents
Method for breeding high-pro-vitamin A content maize inbred lines Download PDFInfo
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- CN103404430A CN103404430A CN2013103577020A CN201310357702A CN103404430A CN 103404430 A CN103404430 A CN 103404430A CN 2013103577020 A CN2013103577020 A CN 2013103577020A CN 201310357702 A CN201310357702 A CN 201310357702A CN 103404430 A CN103404430 A CN 103404430A
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Abstract
The invention provides a method for breeding high-pro-vitamin A content maize inbred lines, which comprises the following steps, obtaining hybrid strains by selecting maize inbred lines with a pro-vitamin A content of greater than or equal to 5 mu g/g as a donor parent, and selecting low-vitamin A-source-content maize inbred lines with an excellent field adaptability as a receptor parent; after carrying out back cross on the hybrid strains and the receptor parent, planting the obtained seeds; planting a hybrid BClF2 family in an isolation area, eliminating obvious diseased or infected plants, carrying out natural pollination on the obtained plants; carrying out hybrid harvesting on the obtained plants; and carrying out homozygosis on the obtained products so as to obtain high-pro-vitamin A maize inbred lines with stable and consistent characters and a pro-vitamin A content of greater than 11.5 mu g/g. The invention also provides a high-pro-vitamin A maize inbred line bred by using the method. According to the method, an obtained maize variety is rich in a pro-vitamin A, and has no linkage inheritance of deleterious trait genes such as susceptible genes.
Description
Technical field
The invention belongs to the agricultural breeding field, particularly the selection of a kind of homovitamin A source corn inbred line.
Background technology
The corn of producing at present upper plantation is the conventional corn kind mostly, nutritional quality is poor, lack human body and nonruminant grow essential lysine, tryptophan and vitamin A and iron etc., take the microelement deficiencies such as crowd nutritional deficiency, particularly vitamin A of corn as staple food thereby cause.VAD is the interior great public disease of a kind of worldwide that the United Nations's health organization is generally acknowledged, approximately 100,000,000 people of developing country just deeply hurt, especially women and children.The caused ophthalmoxerosis that VAD is serious and nyctotyphlosis.Malnutritive not only give the poverty-stricken area crowd healthy brought impact, also causes the tremendous economic loss simultaneously.
At present, the Southwestern China mountain area also has greatly the crowd to take corn to be staple food, and take the common white corn as main.The main source of vitamin A is animal food.Due to backward, animal food intake wretched insufficiency, the phenomenon of VAD quite general (section Chile etc., 2007).
Utilize corn to carry out biological strengthening research as mode crop and be significant, by GENERALIZATION OF MODERN BREEDING TECHNIQUE, improving corn kernel vitamin a source content is the main path (Wurtzel, 2010) that solves global VAD problem.In corn kernel, directly do not contain vitamin A, enrich vitamin a source-carotenoid but contain.In human body, carotenoid transforms can form vitamin A.In white maize, the content of carotenoid is almost nil, but in yellow corn, is containing (the Chander et al.2008b of vitamin a source genetic variation very widely; Egesel et al.2003; Kurilich and Juvik1999; Menkir et al.2008).Carotenoid difference is but very large, and the vitamin a source content of the inbred line of using in breeding at present only is 1-2 μ g/g, and in some donor materials, vitamin a source content is up to 10 μ g/g (Pfeiffer and McClafferty2007; Pixley et al.2010).Utilize existing germ plasm resource by the homovitamin A source proterties transformation in donor to the corn inbred line that can select the homovitamin A source in the acceptor with good economical character; By the combination of different inbred lines, can make the carotenoid content of F1 reach higher, this seed selection for high-load carotenoid corn provides may.
The method of existing seed selection homovitamin A source corn inbred line is mainly to adopt the method for two ring choosing systems, and namely after donor and acceptor hybridization, selfing on earth, finally obtains the corn inbred line in homovitamin A source.Yet, having now and utilizing two ring choosings is the method for seed selection homovitamin A source corn inbred line, because being all generally uses 2 hybridizations between selfed lines, offspring's self cross and selection, cause that easily the breeding material hereditary basis is narrow, the corn variety of follow-up incubation has high certain disease of sense and causes the risk of large loss.And homovitamin A source precursor related gene often with some bad character genes (as susceptible gene etc.) linkage inheritance.Therefore, in the industry in the urgent need to a kind of method of new seed selection homovitamin A source corn inbred line is provided, to overcome the existing problem of prior art.
Summary of the invention
In order to solve the technical problem existed in prior art, the present invention proposes the method for a kind of seed selection homovitamin A source corn inbred line, and it comprises the steps:
Choose corn inbred line that vitamin a source content is more than or equal to 5 μ g/g as donor parents, select adaptability performance excellent low vitamin a source content corn inbred line in field as receptor parent, obtain crossbreed;
By crossbreed and the receptor parent rear plantation BClF1 family that backcrosses;
In the BClF2 family that the isolated area plantation mixes, eliminate obvious diseased plant, open-pollinated, mix results;
Isozygoty, the vitamin a source content that obtains the proterties stable and consistent is greater than the corn inbred line of 11.5 μ g/g.
The method of the seed selection homovitamin A source corn inbred line that the present invention proposes, it comprises the steps:
Choose corn inbred line that vitamin a source (pVAC) content is more than or equal to 5 μ g/g as donor parents, select adaptability performance excellent low vitamin a source content corn inbred line in field as receptor parent, obtain first-filial generation (F1);
F1 and receptor parent are backcrossed, plantation BClF1 family;
According to field adaptability, select in the BClF1 family 5～50%, preferably 10～30%, the individual plant of proterties optimum, pedigree method results;
According to pVAC content in seed, from fruit ear, selecting 2～20%, preferred 5-10%, the fruit ear that pVAC content is the highest mixes;
In the BClF2 family that the isolated area plantation mixes, eliminate obvious diseased plant, open-pollinated, mix results;
Plantation mixes the BClF3 family of results, and each individual plant and Testers hybridization selfing are reserved seed for planting;
Multiple spot is identified the test cross kind, according to the performance of test cross kind, selects 2～20%, preferably 5～15%, more preferably 10%, and the individual plant of proterties optimum, the single-strain seed of middle choosing are in isolated area mixed planting, and open-pollinated, mix results;
Plantation BClF4 mixes seed flowering stage and the haploid inducing line hybridized induction monoploid of results, the monoploid material obtained carries out genome duplication and obtains the corn inbred line that isozygotys, measure pVAC content, choose the corn inbred line that vitamin a source (pVAC) content is greater than 11.5 μ g/g, be the corn inbred line in homovitamin A source.
Described breeding method, wherein utilize high performance liquid chromatography (HPLC), chemical titration, Ultra Performance Liquid Chromatography (UPLC) and/or near infrared spectroscopy (NIR) to measure the content of vitamin a source.
Described breeding method, wherein choose vitamin a source content and be the corn inbred line of 5.0～10.0ug/g as donor parents.
Above related percentage is with respect to the percentage of plant, fruit ear or seed total amount separately, the excellent corn inbred line of described field adaptability performance refers to and can reach more than 3 grades local corn Major Diseases resistance, and it is above that self output reaches 300kg/ mu.Described proterties optimum refers to that output is the highest and can reach more than 3 grades local corn Major Diseases resistance.
The present invention also provides a kind of homovitamin A source corn inbred line by the seed selection of preceding method institute.
The accompanying drawing explanation
Fig. 1 is based on the exemplary breeding line spectrogram of the inventive method.
Embodiment
The present invention is described in detail below in conjunction with accompanying drawing.
Embodiment:
The Breeding Process of homovitamin A source corn inbred line
Take respectively hv12, hv13, hv15 and the hv28 donor (in Table 1) as the homovitamin A source precursor in March, 2009; With CML161, GML33, two M9 and M9 totally 4 selfings that local adaptation is good be acceptor (in Table 1), the method configuration combination by NC II experimental scheme, obtain 16 hybrid combinations altogether.
In August, 2009, backcross 16 F1 and receptor parent, plants 16 BClF1 familys, selects to account for respectively in the BClF1 family according to field adaptability:
5% (family of hv12 donor);
10% (family of hv13 donor),
30% (family of hv15 donor),
The individual plant of the proterties optimum of 50% (family of hv28 donor),
The pedigree method results.
According to pVAC content in seed, in following ratio from each family, selecting respectively the highest fruit ear of pVAC content to mix,
2% (family of hv12 donor);
5% (family of hv13 donor),
10% (family of hv15 donor),
20% (family of hv28 donor).
In March, 2010.4 isolated areas, each isolated area plantation derives from same donor, mixes the BClF2 family of results, eliminates obvious diseased plant, open-pollinated, and each isolated area is mixed results.Break bad gene linkage.
In August, 2010, the donor parents of take is unit, and plantation mixes the BClF3 family of results in right amount respectively, and each individual plant and Testers hybridization selfing are reserved seed for planting, and seed is placed in freezer and uses after the evaluation of test cross kind.
In March, 2011, multiple spot is identified the test cross kind, and according to the performance of test cross kind, each family is selected respectively optimum individual plant in following ratio, and the single-strain seed of middle choosing is in isolated area mixed planting, and open-pollinated, mix results,
5% (family of hv12 donor);
10% (family of hv13 donor),
10% (family of hv15 donor),
20% (family of hv28 donor).
In August, 2011, plant respectively BClF4 and mix the seed of results, flowering stage and haploid inducing line hybridized induction monoploid, the monoploid material obtained carries out genome duplication and obtains the corn inbred line that isozygotys, measure pVAC content, choose the corn inbred line that vitamin a source (pVAC) content is greater than 11.5 μ g/g, can obtain corn inbred line HPV68, HPV76, HPV90 and the HPV98 (seeing attached list) in homovitamin A source.
Table 1 test reaches the new inbred line information that is bred as with corn inbred line
New corn inbred line HPV68, HPV76, HPV90 and the HPV98 be bred as, vitamin a source content is respectively 12.95,12.92,11.51 and 13.96 μ g/g, and vitamin a source content improves obviously.
With the homovitamin A source corn inbred line that the method that two ring choosings are is bred as, easily feel fringe Maize Ear Rot (illustrating that the related gene of controlling corn homovitamin A source and the related gene of controlling Maize Ear Rot have the trend of linkage inheritance), and this situation seldom appears in the homovitamin A source corn inbred line of this method incubation.In 4 colonies (16 combinations) of our configuration, the Hv15 of only take finds the sense Maize Ear Rot in the breeding system of donor, this scheme has finally obtained 12 parts of corn inbred lines that isozygoty, only has a sense Maize Ear Rot, account for 8.3%, finally selected the good homovitamin A source corn inbred line of this part of HPV90.And if by the method for two ring choosing systems, susceptible probability tends to reach more than 55%.
Claims (9)
1. the method for a seed selection homovitamin A source corn inbred line, it comprises the steps:
Choose corn inbred line that vitamin a source content is more than or equal to 5 μ g/g as donor parents, select adaptability performance excellent low vitamin a source content corn inbred line in field as receptor parent, obtain crossbreed;
By crossbreed and the receptor parent rear plantation BClF1 family that backcrosses;
In the BClF2 family that the isolated area plantation mixes, eliminate obvious diseased plant, open-pollinated, mix results;
Isozygoty, obtain the corn inbred line that vitamin a source content is greater than the homovitamin A source of 11.5 μ g/g of getting of proterties stable and consistent.
2. method according to claim 1, it comprises the steps:
Choose corn inbred line that vitamin a source content is more than or equal to 5 μ g/g as donor parents, select adaptability performance excellent corn inbred line in field as receptor parent, obtain F1;
F1 and receptor parent are backcrossed, plantation BCl F1 family;
According to field adaptability, select the individual plant of 5～50% proterties optimums in BCl F1 family, the pedigree method results;
According to vitamin a source content in seed, mix from fruit ear, selecting 2～20% fruit ears that vitamin a source content is the highest;
In the BClF2 family that the isolated area plantation mixes, eliminate obvious diseased plant, open-pollinated, mix results;
Plantation mixes the BClF3 family of results, and each individual plant and Testers hybridization selfing are reserved seed for planting;
Multiple spot is identified the test cross kind, according to the performance of test cross kind, selects the individual plant of 2～20% proterties optimums, and the single-strain seed of middle choosing is in isolated area mixed planting, and open-pollinated, mix results;
Plantation BClF4 mixes seed flowering stage and the haploid inducing line hybridized induction monoploid of results, the monoploid material obtained carries out genome duplication and obtains the corn inbred line that isozygotys, get the corn inbred line that vitamin a source content is greater than 11.5 μ g/g, be the corn inbred line in homovitamin A source.
3. method according to claim 1 and 2, wherein select the individual plant of 10～30% proterties optimums in the BClF1 family, pedigree method results according to field adaptability.
4. method according to claim 2, wherein according to vitamin a source in seed, mix from fruit ear, selecting the fruit ear that 5-10% vitamin a source content is the highest.
5. method according to claim 2, wherein multiple spot is identified the test cross kind, and the individual plant of 5～15% proterties optimums is selected in performance according to the test cross kind, and the single-strain seed of middle choosing is in isolated area mixed planting, and open-pollinated, mix results.
6. method according to claim 5, wherein show according to the test cross kind individual plant of selecting 10% proterties optimum, and the single-strain seed of middle choosing is in isolated area mixed planting, and open-pollinated, mix results.
7. method according to claim 1 and 2, wherein utilize high performance liquid chromatography, chemical titration, Ultra Performance Liquid Chromatography and/or near infrared spectroscopy to measure vitamin a source content.
8. method according to claim 1 and 2, wherein choose vitamin a source content and be the corn inbred line of 5 μ g/g～10.0 μ g/g as donor parents.
9. homovitamin A source corn inbred line, it is the seed selection of either method institute in claim 1-8.
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Cited By (4)
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| CN106613913A (en) * | 2016-12-23 | 2017-05-10 | 天津农学院 | Near infrared-intermediate infrared rapid selection method for corn inbred line combination selection |
| CN106577267B (en) * | 2016-12-23 | 2018-07-20 | 天津农学院 | The gas chromatography mass spectrometry rapid screening method of corn inbred line combination selection |
| CN108834885A (en) * | 2018-05-25 | 2018-11-20 | 吉林省农业科学院 | A kind of corn inbred lines method |
| CN109588308A (en) * | 2019-01-22 | 2019-04-09 | 重庆市农业科学院 | A kind of selection of VII type amylomaize material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106577267B (en) * | 2016-12-23 | 2018-07-20 | 天津农学院 | The gas chromatography mass spectrometry rapid screening method of corn inbred line combination selection |
| CN108834885A (en) * | 2018-05-25 | 2018-11-20 | 吉林省农业科学院 | A kind of corn inbred lines method |
| CN109588308A (en) * | 2019-01-22 | 2019-04-09 | 重庆市农业科学院 | A kind of selection of VII type amylomaize material |
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Inventor after: Wu Yongsheng Inventor after: Huang Kaijian Inventor after: Tan Hua Inventor after: Huang Aihua Inventor after: Wei Xinxing Inventor after: Zheng Debo Inventor after: Zou Chenglin Inventor after: Mo Runxiu Inventor before: Wu Yongsheng Inventor before: Huang Kaijian Inventor before: Tan Hua Inventor before: Huang Aihua Inventor before: Wei Xinxing Inventor before: Zheng Debo Inventor before: Zhou Chenglin Inventor before: Mo Runxiu |
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