CN106613913B - Infrared rapid screening method in the near-infrared-of corn inbred line combination selection - Google Patents
Infrared rapid screening method in the near-infrared-of corn inbred line combination selection Download PDFInfo
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- CN106613913B CN106613913B CN201611207172.1A CN201611207172A CN106613913B CN 106613913 B CN106613913 B CN 106613913B CN 201611207172 A CN201611207172 A CN 201611207172A CN 106613913 B CN106613913 B CN 106613913B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of corn inbred line combination selection it is close in infrared rapid screening method, step is：(1) preparing experiment corn inbred line sample, including control male parent sample A and maternal sample B；(2) near infrared spectrum and middle infrared spectrum of prepared sample are scanned；(3) obtained near infrared spectrum and middle infrared spectrum are merged, the nearly mid-infrared light spectrum matrix after being merged；(4) the nearly mid-infrared light spectrum matrix of principal component analysis is used；(5) it screens and determines and compare sample similar in male parent sample A and maternal sample B；(6) apolegamy is combined to identified similar sample.The present invention overcomes the non-directiveness that traditional breeding way selects, basis is provided for the selection of parent in follow-up breeding process, this method is simple, science, screening efficiency are high, in high volume, quickly can go out the combination of target corn inbred line in early screening, save a large amount of manpower, material resources cost.
Description
Technical field
The invention belongs to crop seeds selection and breeding field, more particularly to a kind of corn inbred line combination selection it is close-in
Infrared rapid screening method.
Background technology
Corn is one of three big crop of the world, and seed demand is huge.By crop breeding Crop Improvement hereditary capacity,
It is to improve crop yield, quality, one of the effective way to improve People's livelihood of promoting economic development to cultivate fine quality.Current breeding
Mode includes mainly crossbreeding, the methods of back cross breeding, mostly uses pedigree method to select excellent generation and heterosis, hybrid vigor strong
Parent, spent time is long, and since in field trial, experimental condition is harsh, is highly prone to the influence of environmental condition, and
And due to the non-directional of traditional breeding way selection, cause the quantities such as collection seed great, extremely wasting manpower and material resources' resource.
Therefore, development is a kind of in high volume, in real time, quickly capable of going out the combination of target corn inbred line for breeding in early screening
Analysis method is very important.
Due to near-infrared, middle infrared spectrum characterize be organic molecule hydric group (C-H, N-H, O-H etc.) fundamental frequency, times
Frequently, sum of fundamental frequencies absorbs, and can provide the information such as molecular structure and composition state (such as protein, amino acid, starch and fat), therefore
Near-infrared, middle infrared spectrum as a kind of efficient, quick modern analytical technique, with its non-destructive, speed it is fast, at low cost etc.
Feature is to be widely used in quality of agricultural product detection.But near-infrared, middle infrared spectrum are used for corn inbred line group
The technology for closing apolegamy is not reported.
Invention content
For the deficiencies in the prior art, the present invention provides a kind of simple, science, screening efficiency high corn selfing
It is the near-to-mid infrared rapid screening method of combination selection.
The present invention is achieved by the following technical solutions：
Infrared rapid screening method in a kind of near-infrared-of corn inbred line combination selection, step are：
(1) multiple corn inbred line samples of 80 mesh particle sizes of preparing experiment, including control male parent sample A and female parent
Sample B；
(2) near infrared spectrum and middle infrared spectrum of sample prepared by scanning step (1), obtains experiment corn inbred line
The near infrared spectrum and middle infrared spectrum of sample；
(3) near infrared spectrum and middle infrared spectrum that step (2) obtains are merged, the experiment after being merged is beautiful
Rice self-mating system sample near-infrared-mid-infrared light spectrum matrix；
(4) principal component analysis experiment corn inbred line sample near-infrared-mid-infrared light spectrum matrix is used, experiment is obtained and uses
The shot chart of corn inbred line sample near-infrared-mid-infrared light spectrum matrix；
(5) the experiment corn inbred line sample near-infrared-mid-infrared light spectrum matrix shot chart obtained according to step (4),
It screens and determines and compare sample similar in male parent sample A and maternal sample B；
(6) to determined by step (5) with compare sample similar in male parent sample A and maternal sample B and be combined apolegamy.
Moreover, the wave band that the near infrared spectrum uses is 4000-10000cm-1.
Moreover, the wave band that the middle infrared spectrum uses is：650-4000cm-1.
It advantages of the present invention and has the beneficial effect that：
(1) the near-to-mid infrared rapid screening method of corn inbred line combination selection of the invention, relative to traditional breeding
Process in high volume, quickly can go out the combination of target corn inbred line in early screening.
(2) the near-to-mid infrared rapid screening method of corn inbred line combination selection of the invention, relative to traditional breeding
Process can save a large amount of manpower, material resources cost.
(3) method of the invention is simple, science, screening efficiency are high, extends to the self-mating system combination selection of other crops
In.
Description of the drawings
Fig. 1 is the near infrared spectrum of 33 corn inbred line samples in the embodiment of the present invention；
Fig. 2 is the middle infrared spectrum of 33 corn inbred line samples in the embodiment of the present invention；
Fig. 3 is the principal component scores of 33 corn inbred line sample near-infrared-middle infrared spectrums in the embodiment of the present invention
Figure.
Specific implementation mode
The present invention is described in further detail by following embodiment.It should be noted that：Following embodiments are illustrative, are not
It is limited, protection scope of the present invention cannot be limited with following embodiments.
A kind of near-to-mid infrared rapid screening method of corn inbred line combination selection, its step are as follows：
(1) 33 corn inbred line samples (serial number No.1-No.33) of 80 mesh particle sizes of preparing experiment, including it is right
According to male parent sample No.11 and maternal sample No.20；
(2) near infrared spectrum and middle infrared spectrum of 33 corn inbred line samples prepared by scanning step (1), obtains
The near infrared spectrum and middle infrared spectrum of experiment corn inbred line sample；Near infrared spectrum scanning wave-number range is 4000-
10000cm-1, it is 650-4000cm that middle infrared spectrum, which scans wave-number range,-1；
(3) near infrared spectrum of 33 corn inbred line samples that step (2) obtains and middle infrared spectrum are merged,
Near-infrared-mid-infrared light spectrum matrix of 33 corn inbred line samples after being merged；
(4) infrared in the near-infrared-of the 33 corn inbred line samples obtained using Principal Component Analysis analytical procedure (2)
Spectrum matrix obtains the shot chart of 33 corn inbred line sample near-to-mid infrared spectrum matrixes；
(5) shot chart of the 33 corn inbred line sample near-to-mid infrared spectrum matrixes obtained according to step (4), screening
And it determines and compares sample similar in male parent sample A No.11 and maternal sample No.20；
(6) to determined by step (5) with compare sample progress similar in male parent sample No.11 and maternal sample No.20
Combination selection.
33 corn inbred line samples provide for Hainan seeds company in the present embodiment.Using high speed Universal pulverizer
(FW100) each corn variety is crushed, and crosses 80 mesh sieve, packs standby survey.
Near infrared spectra collection in the present invention uses the Fourier transform near infrared spectrum of PerkinElmer companies of the U.S.
Instrument, InGaAs detectors, instrument carry integrating sphere accessory, spectral scanning range 4000-10000cm-1, resolution ratio 4cm-1,
Sweep spacing is 4cm-1, scanning times 16.The above-mentioned sample prepared is fitted into specimen cup, is flattened, and be placed on integrating sphere
On specimen rotating holder, using reference built in integrating sphere as background, the near-infrared diffusing reflection spectrum of each sample is acquired respectively.In it is red
External spectrum acquisition is carried using the Fourier transform mid-infrared light spectrometer of PerkinElmer companies of the U.S., DTGS detectors, instrument
Decaying be totally reflected (ATR) attachment, scanning range be 650-4 000cm-1, resolution ratio 4cm-1, sweep spacing 4cm-1, sweep
Retouch number 16.Equally, it by the above-mentioned sample prepared, is fitted into atr crystal pond, flattens, with air background, acquire respectively each
The decaying of a sample is totally reflected middle infrared spectrum.
Fig. 1 is 33 corn inbred line samples in 4000-10000cm-1The atlas of near infrared spectra in section.It can from figure
Go out, corn is in 4339cm-1、4755cm-1、5184cm-1、5744cm-1、6837cm-1And 8360cm-1It deposits all in absorption peak at place.Its
In in 5700-5560cm-1, 8316cm-1First and second absorption in over-frequency of the absorption peak essentially from C-H stretching vibrations；5172cm-1And 6840cm-1First and second absorption in over-frequency of the absorption peak essentially from O-H contracting vibrations；4744cm-1And 4266cm-1Absorption peak
It is absorbed essentially from the sum of fundamental frequencies of C-H stretching vibrations and bending vibration.
Fig. 2 is 33 corn inbred line samples in 650-4000cm-1The mid-infrared light spectrogram in section.These samples exist
839cm-1、1006cm-1、1754cm-1、2851cm-1And 2931cm-1There are common characteristic peaks at place, wherein 1754cm-1Place is
Fatty characteristic peak, is mainly caused by carbonylic stretching vibration in corn；2851cm-1And 2931cm-1Locate absorption peak by aliphatic
CH stretching vibrations cause；839cm-1And 1006cm-1Locate absorption peak respectively from the ethylene compounds and primary alconol in corn.
All 33 corn inbred line samples are can be seen that in close in infrared wave-number range from Fig. 1 and Fig. 2, spectrum shape
Shape, the position of characteristic peak are all closely similar, therefore, can not be filtered out according to these collection of illustrative plates and compare male parent sample No.11 and mother
Sample similar in this sample No.20.
In order to effectively screen and compare sample similar in male parent and female parent, by the near-infrared of 33 corn inbred line samples
Spectrum and middle infrared spectrum are merged, the near-to-mid infrared spectrum matrix after being merged, and using Principal Component Analysis to it
It is analyzed.The first two principal component PC1 and PC2 explain that 73.43% and 21.56% original spectrum variable, Fig. 3 are 33 jade respectively
The shot chart of rice self-mating system near-to-mid infrared spectrum matrix the first two principal component PC1 and PC2.From figure 3, it can be seen that 33 jade
Rice self-mating system is divided into two major classes, and one kind is located at the negative regions PC1, in addition a kind of to be located at the positive regions PC1.And compare male parent sample
No.11 is located at the negative regions PC1, and the sample away from nearest neighbours is：No.32, No.26 and No.8；And compare maternal sample
No.20 is located at the positive regions PC1, and the sample away from nearest neighbours is：No.31, No.22 and No.21.It is preliminary by the above method
It filters out：Corn inbred line sample No.32, No.26 and No.8 can as new male parent, corn inbred line sample No.31,
No.22 and No.21 can be as new female parent.It is selected with compare male parent sample No.11 similar sample No.32, No.26
And No.8, it can mutually be assembled between maternal sample No.20 similar sample No.32, No.26 and No.8 with compareing.
It is above-mentioned with reference to embodiment to the detailed description of the near-to-mid infrared rapid screening method of corn inbred line combination selection,
It is illustrative without being restrictive, it should explanation is any simple in the case where not departing from core of the invention
Deformation, modification or other skilled in the art can not spend the equivalent replacement of creative work to each fall within guarantor of the invention
Protect range.
Claims (3)
1. infrared rapid screening method in a kind of near-infrared-of corn inbred line combination selection, it is characterised in that including following step
Suddenly：
(1) multiple corn inbred line samples of 80 mesh grain sizes of preparing experiment, including control male parent sample A and maternal sample B；
(2) near infrared spectrum and middle infrared spectrum of sample prepared by scanning step (1), obtains experiment corn inbred line sample
Near infrared spectrum and middle infrared spectrum；
(3) near infrared spectrum and middle infrared spectrum that step (2) obtains are merged, the experiment after being merged with corn from
Friendship is sample near-infrared-mid-infrared light spectrum matrix；
(4) it uses Principal Component Analysis to analyze experiment corn inbred line sample near-infrared-mid-infrared light spectrum matrix, is tested
With the shot chart of corn inbred line sample near-infrared-mid-infrared light spectrum matrix；
(5) the experiment corn inbred line sample near-infrared-mid-infrared light spectrum matrix shot chart obtained according to step (4), screening
And it determines and compares sample similar in male parent sample A and maternal sample B；
(6) to determined by step (5) with compare sample similar in male parent sample A and maternal sample B and be combined apolegamy.
2. the near-to-mid infrared rapid screening method of corn inbred line combination selection according to claim 1, feature exist
In：The wave band of the near infrared spectrum is 4000-10000cm-1。
3. the near-to-mid infrared rapid screening method of corn inbred line combination selection according to claim 2, feature exist
In：The wave band of the middle infrared spectrum is 650-4000cm-1。
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| CN107561031A (en) * | 2017-08-29 | 2018-01-09 | 天津农学院 | Based on two-dimentional near-infrared, in infrared Correlated Spectroscopy information fusion sesame oil in mix corn oil detection method |
| CN109540832A (en) * | 2018-11-23 | 2019-03-29 | 天津农学院 | A kind of detection method based on total nitrogen in the near-to-mid infrared spectrum large-scale milch cow farms liquid manure of fusion |
| CN114166788B (en) * | 2021-11-16 | 2024-02-20 | 华中农业大学 | Mid-infrared rapid batch detection method for total content of free amino acids in milk |
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| CN101819141A (en) * | 2010-04-28 | 2010-09-01 | 中国科学院半导体研究所 | Maize variety identification method based on near infrared spectrum and information processing |
| CN103020864A (en) * | 2012-12-07 | 2013-04-03 | 南通大学 | Corn fine breed breeding method |
| CN103404430A (en) * | 2013-08-16 | 2013-11-27 | 广西壮族自治区农业科学院玉米研究所 | Method for breeding high-pro-vitamin A content maize inbred lines |
| CN105224960A (en) * | 2015-11-04 | 2016-01-06 | 江南大学 | Based on the corn seed classification hyperspectral imagery model of cognition update method of clustering algorithm |
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Patent Citations (4)
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| CN101819141A (en) * | 2010-04-28 | 2010-09-01 | 中国科学院半导体研究所 | Maize variety identification method based on near infrared spectrum and information processing |
| CN103020864A (en) * | 2012-12-07 | 2013-04-03 | 南通大学 | Corn fine breed breeding method |
| CN103404430A (en) * | 2013-08-16 | 2013-11-27 | 广西壮族自治区农业科学院玉米研究所 | Method for breeding high-pro-vitamin A content maize inbred lines |
| CN105224960A (en) * | 2015-11-04 | 2016-01-06 | 江南大学 | Based on the corn seed classification hyperspectral imagery model of cognition update method of clustering algorithm |
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