CN107347634A - A kind of method based on tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize self-mating system - Google Patents
A kind of method based on tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize self-mating system Download PDFInfo
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- CN107347634A CN107347634A CN201710823449.1A CN201710823449A CN107347634A CN 107347634 A CN107347634 A CN 107347634A CN 201710823449 A CN201710823449 A CN 201710823449A CN 107347634 A CN107347634 A CN 107347634A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a kind of method based on tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize self-mating system, described method is in the Breeding Process of pericarp anthocyanidin synthesis type fresh edible maize self-mating system, according to the color trait seed selection pericarp anthocyanidin synthesis type fresh edible maize self-mating system of the tip of a leaf, idiographic flow includes：Hybridization choosing system is carried out using pericarp anthocyanidin synthesis type fresh edible maize self-mating system and the colourless corn inbred line of pericarp, F1 generation selfing is reserved seed for planting, start the color trait according to the tip of a leaf in F2 generations, the individual plant of selection tip of a leaf purple stays seedling selfing to reserve seed for planting, planted by head progeny row, take into account the good individual plant of selection phenotypic character, continuous self cross and selection some generations, you can obtain the pericarp anthocyanidin synthesis type corn inbred line of stable heredity.Compared to prior art, this method can accelerate the seed selection process of pericarp anthocyanidin synthesis type fresh edible maize self-mating system, reduce workload, improve the Breeding Efficiency of colored corn variety.
Description
Technical field
The present invention relates to the technical field of Crop Genetic Breeding, more particularly to one kind to utilize tip of a leaf color trait
The method of assist-breeding pericarp anthocyanidin synthesis type fresh edible maize self-mating system.
Background technology
Anthocyanidin is a kind of water-soluble natural pigment synthesized in plant, and anthocyanidin is a kind of strong antioxidant,
A variety of benefits can be brought for human body, e.g., human body can be protected from the damage of free radical, strengthen blood vessel elasticity, improve cyclic system
System etc..The presence of anthocyanidin, such as corn, rice, sorghum, purple potato have been found that in plurality of cereals crop.
Eat the colored corn such as purple, black raw and be rich in anthocyanidin, because the content of its anthocyanidin is high, fat, protein, iron, calcium, ammonia
The rich content of the nutrients such as base acid, it is considered to be the health care gold food in fresh edible maize.Pericarp is color rich in anthocyanidin
One kind of color fresh edible maize, favor is enjoyed because its F1 generation fruit anthocyanidin content is higher.At present, domestic colored corn germplasm
Resource is extremely limited, turns into wound using corn germplasm of the colourless fresh edible maize orderly improvement pericarp rich in anthocyanidin of pericarp
Make a kind of conventional method of colored corn inbred line, it is expected to make up the deficient short slab of colored corn germ plasm resource, give full play to existing
There is the advantage of corn germ plasm resource, improve the Breeding Efficiency of colored corn variety.However, forefathers cultivate pericarp flower in orderly improvement
During blue or green plain synthesis type fresh edible maize self-mating system, usually because F1 generation, F2 do not produce separation for seed color and luster character and felt confused,
The selection that continues for abandoning progeny material is cultivated, to find out its cause, be due to that corn kernel pericarp is developed by ovary wall, fruit
The character of skin is presented with parent always, and the trait expression of seed is consistent with previous generation, F3 for when side occur full fringe pericarp without
Color and full fringe pericarp purple or single fringe of black, have further delayed pericarp anthocyanidin synthesis type to eat jade raw in F3 initially-separate
The seed selection process of rice self-mating system, adds the workload during Single-plant selection.And if entered again by trait segregation when F3 generations
Row screening, although can reach the purpose of directive breeding, so obviously increases workload, extends breeding cycle, unfavorable
In the quickly breeding of colored fresh edible maize self-mating system new varieties.
The content of the invention
Tip of a leaf color assist-breeding pericarp is based on it is an object of the invention to overcome the deficiencies of the prior art and provide a kind of
The method of anthocyanidin synthesis type fresh edible maize self-mating system, pericarp anthocyanidin synthesis type fresh edible maize is cultivated certainly to solve orderly improvement
When friendship is, the technical problem of breeding cycle length caused by F1 generation, F2 do not produce separation for seed color and luster character.
The present invention is achieved by the following technical solutions：
The invention provides a kind of based on tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize self-mating system
Method, this method carry out hybridization choosing using pericarp anthocyanidin synthesis type fresh edible maize self-mating system with the colourless corn inbred line of pericarp
System, F1 generation selfing are reserved seed for planting, and start the color trait according to the tip of a leaf in F2 generations, select the individual plant of tip of a leaf purple to stay seedling selfing to reserve seed for planting,
Planted by head progeny row, take into account the good individual plant of selection phenotypic character, continuous self cross and selection some generations, you can obtain the fruit of stable heredity
Peel anthocyanidin synthesis type corn inbred line.
Specifically include following steps：
(1) selected from ordinary maize Germplasm Bank the corn inbred line of pericarp black or purple and the colourless corn of pericarp from
Hand over system to be hybridized, obtain F1 generation；
(2) F1 generation selfing is reserved seed for planting, obtains F2 generations；
(3) F2 is selected tip of a leaf color as the individual plant of purple, takes into account the good individual plant of selection phenotypic character for mixed seeding
Carry out selfing and stay single fringe, obtain F3 generations；
(4) in F3 generations, are planted by head progeny row, it is the individual plant of purple that tip of a leaf color is selected in head progeny row, takes into account selection phenotypic character
Good individual plant carries out selfing and stays single fringe, obtains F4 generations；
(5) the seed selection step of offspring's repeat step (4), Fn generations are obtained；
(6) in Fn generations, are planted by head progeny row, it is the individual plant of purple that tip of a leaf color is selected in head progeny row, takes into account selection phenotypic character
Good individual plant, selfing are reserved seed for planting, that is, cultivate the pericarp anthocyanidin synthesis type fresh edible maize self-mating system new varieties for obtaining stable heredity.
Further, in the step (1), the corn inbred line of pericarp black or purple is selfed for corn 0414 or 0425
System, the colourless corn inbred line of pericarp is the self-mating system of corn 3370, derives from academy of agricultural sciences of Anhui Province corn germ plasm resource storehouse.
Further, in the step (5), n=5~7, i.e., tip of a leaf color phenotype character is begun to focus in F2 generations, from fringe
Single fringe of the tip of a leaf color for purple is left and taken in row selection, is planted by single fringe, after the continuous generation of self cross and selection 2~4, obtains stable heredity
Pericarp anthocyanidin synthesis type fresh edible maize self-mating system.
Further, the phenotypic character includes plant type, blade profile, disease resistance.
The present invention has advantages below compared with prior art：Present invention firstly discovers that corn tip of a leaf anthocyanidin and pericarp cyanine
Linkage inheritance relation between element, and using the method for conventional hybridization, there is provided one kind utilizes tip of a leaf color trait assist-breeding
The method of pericarp anthocyanidin synthesis type fresh edible maize self-mating system, it the method overcome orderly improvement and cultivate pericarp anthocyanidin synthesis type
During fresh edible maize self-mating system, the technology of seed selection can not be started in F2 generations because F1 generation, F2 do not produce separation for seed color and luster character
Problem, the accurate selection of colored fresh edible maize self-mating system and the cultivation process of new varieties are accelerated, reduce workload, improved colored
The Breeding Efficiency of corn variety.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
It is selfed the invention provides one kind using tip of a leaf color trait assist-breeding pericarp anthocyanidin synthesis type fresh edible maize
The method of system, comprises the following steps：
(1) corn inbred line 0414 of the pericarp black corn inbred line 3370 colourless with pericarp is hybridized, obtains F1 generation；
(2) F1 generation selfing is reserved seed for planting, obtains F2 generations；
(3) F2 is selected tip of a leaf color as the individual plant of purple, takes into account selection plant type, blade profile, disease resistance etc. for mixed seeding
The good individual plant of phenotypic character carries out selfing and stays single fringe, obtains F3 generations；
(4) in F3 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character carries out selfing and stays single fringe, obtains F4 generations；
(5) in F4 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character carries out selfing and stays single fringe, obtains F5 generations；
(6) in F5 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character carries out selfing and stays single fringe, obtains F6 generations；
(7) in F6 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character carries out selfing and stays single fringe, obtains F7 generations；
(8) in F7 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character, selfing are reserved seed for planting, and obtain the pericarp anthocyanidin synthesis type fresh edible maize of stable heredity
Self-mating system, 25 parts altogether.
Embodiment 2
The invention provides another kind to utilize tip of a leaf color trait assist-breeding pericarp anthocyanidin synthesis type fresh edible maize certainly
The method for handing over system, comprises the following steps：
(1) corn inbred line 0425 of the pericarp purple corn inbred line 3370 colourless with pericarp is hybridized, obtains F1 generation；
(2) F1 generation selfing is reserved seed for planting, obtains F2 generations；
(3) F2 is selected tip of a leaf color as the individual plant of purple, takes into account selection plant type, blade profile, disease resistance etc. for mixed seeding
The good individual plant of phenotypic character carries out selfing and stays single fringe, obtains F3 generations；
(4) in F3 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character carries out selfing and stays single fringe, obtains F4 generations；
(5) in F4 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character carries out selfing and stays single fringe, obtains F5 generations；
(6) in F5 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character carries out selfing and stays single fringe, obtains F6 generations；
(7) in F6 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character, selfing are reserved seed for planting, and obtain the pericarp anthocyanidin synthesis type fresh edible maize of stable heredity
Self-mating system, 19 parts altogether.
Embodiment 3
The invention provides another kind to utilize tip of a leaf color trait assist-breeding pericarp anthocyanidin synthesis type fresh edible maize certainly
The method for handing over system, comprises the following steps：
(1) corn inbred line 0414 of the pericarp black corn inbred line 3370 colourless with pericarp is hybridized, obtains F1 generation；
(2) F1 generation selfing is reserved seed for planting, obtains F2 generations；
(3) F2 is selected tip of a leaf color as the individual plant of purple, takes into account selection plant type, blade profile, disease resistance etc. for mixed seeding
The good individual plant of phenotypic character carries out selfing and stays single fringe, obtains F3 generations；
(4) in F3 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character carries out selfing and stays single fringe, obtains F4 generations；
(5) in F4 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character carries out selfing and stays single fringe, obtains F5 generations；
(6) in F5 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, take into account selection plant type, leaf
The good individual plant of type, disease resistance isophenous character, selfing are reserved seed for planting, and obtain the pericarp anthocyanidin synthesis type fresh edible maize of stable heredity
31 parts of self-mating system.
Be above a kind of detailed embodiment of the invention and specific operating process, be using technical solution of the present invention before
Put and implemented, method therefor is known to those skilled in the art the conventional method of dawn unless otherwise instructed, involved
Germ plasm resource, derive from the corn germ plasm resource storehouse of Tobacco Research Institute, Anhui Academy of Agricultural Sciences, but the protection of the present invention
Scope is not limited to the above embodiments.
Claims (5)
1. a kind of method based on tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize self-mating system, its feature exist
In step includes：Hybridized using the pericarp anthocyanidin synthesis type fresh edible maize self-mating system corn inbred line colourless with pericarp
Choosing system, F1 generation selfing are reserved seed for planting, and start the color trait according to the tip of a leaf in F2 generations, select the individual plant of tip of a leaf purple to stay seedling selfing to stay
Kind, planted by head progeny row, take into account the good individual plant of selection phenotypic character, in continuous self cross and selection some generations, obtain stable hereditary fruit
Peel anthocyanidin synthesis type corn inbred line.
It is 2. according to claim 1 a kind of based on the selfing of tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize
The method of system, it is characterised in that the described method comprises the following steps：
(1) corn inbred line of pericarp black or purple and the colourless corn inbred line of pericarp are selected from ordinary maize Germplasm Bank
Hybridized, obtain F1 generation；
(2) F1 generation selfing is reserved seed for planting, obtains F2 generations；
(3) F2 is selected tip of a leaf color as the individual plant of purple, takes into account the good individual plant of selection phenotypic character and carry out for mixed seeding
Single fringe is stayed in selfing, obtains F3 generations；
(4) in F3 generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, it is good to take into account selection phenotypic character
Individual plant carry out selfing and stay single fringe, obtain F4 generations；
(5) the seed selection step of offspring's repeat step (4), Fn generations are obtained；
(6) in Fn generations, are planted by head progeny row, tip of a leaf color is selected in head progeny row as the individual plant of purple, it is good to take into account selection phenotypic character
Individual plant, selfing reserves seed for planting, and obtains the pericarp anthocyanidin synthesis type fresh edible maize self-mating system kind of stable heredity.
It is 3. according to claim 2 a kind of based on the selfing of tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize
The method of system, it is characterised in that in the step (1), the corn inbred line of pericarp black or purple is corn 0414 or 0425
Self-mating system, the colourless corn inbred line of pericarp are the self-mating system of corn 3370.
It is 4. according to claim 2 a kind of based on the selfing of tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize
The method of system, it is characterised in that in the step (5), n=5~7.
It is 5. according to claim 1 a kind of based on the selfing of tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize
The method of system, it is characterised in that the phenotypic character includes plant type, blade profile, disease resistance.
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| CN201710823449.1A CN107347634B (en) | 2017-09-13 | 2017-09-13 | A method of based on tip of a leaf color assist-breeding pericarp anthocyanidin synthesis type fresh edible maize self-mating system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110731264A (en) * | 2019-11-22 | 2020-01-31 | 山西大丰种业有限公司 | method for identifying hybrid plants in corn haploid plants |
| CN111165344A (en) * | 2020-02-26 | 2020-05-19 | 广东省农业科学院作物研究所 | Method for rapidly creating sweet corn inbred line rich in anthocyanin |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101663995A (en) * | 2009-09-25 | 2010-03-10 | 沈阳农业大学 | Selective breeding technique of anthocyanin type purple corn |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101663995A (en) * | 2009-09-25 | 2010-03-10 | 沈阳农业大学 | Selective breeding technique of anthocyanin type purple corn |
Non-Patent Citations (2)
| Title |
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| 史振声等: "鲜食玉米新品种选育原理及技术技巧", 《玉米科学》 * |
| 贾耀唐等: "《作物遗传育种》", 31 March 1984 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110731264A (en) * | 2019-11-22 | 2020-01-31 | 山西大丰种业有限公司 | method for identifying hybrid plants in corn haploid plants |
| CN110731264B (en) * | 2019-11-22 | 2022-07-26 | 山西大丰种业有限公司 | Method for identifying hybrid plants in corn haploid plants |
| CN111165344A (en) * | 2020-02-26 | 2020-05-19 | 广东省农业科学院作物研究所 | Method for rapidly creating sweet corn inbred line rich in anthocyanin |
| CN111165344B (en) * | 2020-02-26 | 2022-01-18 | 广东省农业科学院作物研究所 | Method for rapidly creating sweet corn inbred line rich in anthocyanin |
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