CN102511382A - Method for breeding single cross hybrid of rough dwarf-resistant fresh sweet waxy maize - Google Patents
Method for breeding single cross hybrid of rough dwarf-resistant fresh sweet waxy maize Download PDFInfo
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Abstract
The invention discloses a method for breeding single cross hybrid of rough dwarf-resistant fresh sweet waxy maize, which comprises the following steps: selecting waxy maize germ-plasm Tongxi 5 to form a breeding basic group, continuously selfing the breeding basic group, and breeding to obtain a selfed line as a female parent of a hybrid; selecting waxy maize germ-plasm Hengbai 522, high-resistance germ-plasm T877 containing tropical germ-plasm and medium-resistance germ-plasm Putian 84, crossing with each other, and mixing the crossing combinations at an equal seed amount to form an original breeding group as a basic group for male parent line selection; breeding fine selfed lines; selecting three recessive homozygote excellent lines using a three-step method: identifying the maize rough dwarf resistance of the above three recessive genotype homozygotes using crossing combinations, and selecting seeds with maize rough dwarf resistance as a male parent of the hybrid; and hybridizing the obtained female parent and the male parent, and breeding to obtain a single cross hybrid. The method disclosed by the invention is easy in operation, and the bred maize variety has good appearance quality and eating quality and has excellent rough dwarf resistance.
Description
Technical field
The present invention relates to a kind of anti-rough dwarf disease and eat sweet-waxy maizes single cross hybrid selection raw.
Background technology
Waxy corn originates from China, so claim Chinese glutinous type corn (Chinese waxy corn).Because it contains waxy starch; Thereby determined it to have the industrialization potential higher than sweet corn; Both can be on starch the formation industry; Also can carry out high nutrition, the industrialization development of high-quality fresh edible maize in each side such as good palatability, high-lysine content, particularly China's most of areas waxy corn is main to eat raw for a long time, carries out in China and eats glutinous corn breeding and industrialization development raw and have advantageous advantage and have a extensive future.MRDV is the disease that has a strong impact on China's Maize Production in recent years, some local grave illness time even cause into ten thousand hectares of total crop failures.Can be on the conventional corn through ahead of time or delay sowing phase of causing a disease of staggering and reach the method for evading the rough dwarf disease evil and reduce the loss; Eat the necessity that waxy corn has determined its anniversary plantation raw; Even or the serious phase sowing of causing a disease of staggering; All eat waxy corn raw still will draw back sowing time as far as possible to alleviate circulating pressures such as concentrated market sale of going on the market and working ability restriction, carries out anti-rough dwarf disease and eats waxy corn single cross hybrid seed selection necessity and urgent raw.At present domestic anti-rough dwarf disease germ plasm resource is poor, focuses mostly on and is utilizing 78599 germplasms.
Summary of the invention
The object of the present invention is to provide the anti-rough dwarf disease of a kind of high-quality, tool rough dwarf disease resistance to eat sweet-waxy maizes single cross hybrid selection raw.
Technical solution of the present invention is:
A kind of anti-rough dwarf disease is eaten sweet-waxy maizes single cross hybrid selection raw, it is characterized in that: comprise the following steps:
(1) selecting the waxy corn germplasm logical is 5 to form [logical is 5 * Shendan No.7 number white grain variant) logical be 5] breeding basic population, and by the continuous selfing of this breeding basic population, seed selection becomes the female parent of inbred line as crossbreed again; Said [logical is 5 * Shendan No.7 number white grain variant) logical be 5] breeding basic population be select for use in two generations of Shendan No.7 selfing white grain variant with logical be the crossbreed that No. 5 hybridization obtains, again with logical be the breeding basic population of backcrossing and obtaining for No. 5;
(2) select the waxy corn germplasm weigh white 522 with the high anti-germplasm T877 that contains tropical germplasm, in anti-germplasm general sweet 84, the three is hybridized in twos, each hybrid combination mixes by the equivalent seed and forms original breeding population, as the basic population of male parent choosing system;
(3) the good inbred line of seed selection: original breeding population is fully mixed powder, obtain mixing powder colony, chain to break; Then from mixing powder colony selfing 50 ~ 100 fringes, selfing limit, limit is with non-assorted excellent crowd's inbred line test cross, according to output, the resistance index of test cross kind, and the final election major clique, and major clique fully mixed powder generation breeding population; The continuous selfing of above-mentioned breeding population is produced inbred line make the male parent group;
(4) utilize three-step approach final election three recessive homozygote major cliques:
The fruit ear of the segregative generation of the male parent group that the first step obtains from step (3) is selected glutinous grain; The choosing from the separation offspring of glutinous property seed of second step obtains sweet grain, in its sweet grain the sweet seed of general sweet and general sweet double reinforcement is arranged, and chooses earlier and does the slow person of not plump speed in the air-dry seed, and preliminary choosing obtains the sweet seed of general sweet double reinforcement; Under the 3rd sweet seed kind of general sweet double reinforcement of step with primary election, bright fringe field harvest time adopt half fringe or 1/3 fringe to taste to confirm sugariness high be three recessive gene type homozygotes;
(5) adopt hybrid combination to identify the homozygotic MRDV resistance of above-mentioned three recessive gene types, select to have the male parent of the seed of MRDV resistance as crossbreed;
(6) female parent that step (1) is obtained becomes single cross hybrid with the paternal hybrid seed selection that step (5) obtains.
The method of the glutinous grain of first step selection is to select endosperm to be the seed of wax in the step (4).
The method of the sweet grain of second step selection is that the seed of selection profile shrinkage is sweet grain in the step (4).
The corn that the present invention selects has following advantage:
1. exterior quality and edible quality are good.Corn exterior quality and edible quality that application the inventive method is bred are superior to contrast (Suyunuo No.1), and bright tassel seed sugariness is high, skin is thin, seed is bigger; Fruit ear seed marshalling, it is complete not bald seed; Seed color and luster white is evenly beautiful after the boiling; The fruit ear bract is complete and tighter, and a small amount of boot leaf is arranged, and keeps the shelf life of fine quality longer.
2. Other Main Agronomic Characters is good, shows as high yield, stable yields.Because making up used waxy corn, sweet corn and the conventional corn germplasm of breeding basic population not only has high-quality; And derive from different areas or planted in different ecological areas; Has blood relationship far away; The different hybrid vigour crowd of design parents is with the seed selection single cross hybrid, guaranteed stable high yield and the adaptability of improved crossing kind etc.General per mu yield Chinese olive fringe is 800～1000 ㎏, and the high-yield field can reach 1300 ㎏, and comparison is according to glutinous No. 5 raising the output about 10% of Su Yu.The sowing of sowing in spring is about about 94 days to gathering, and is similar with No. 1 ripe phase of Su Yu (glutinous).Growth potential is strong, and the plant type hemicompact is gathered, and regularity is better, and about plant height 220-240cm, about the 110cm of fringe position, dual spike rate about 6% becomes about 19 of strain total leaf numbers; Plant type is compact, ripe lover of later stage; Fruit ear is conical, about spike length 17-18 ㎝, and about bald long 1-1.5 cm, about the thick 4.5-4.6cm of fringe, cob white, about tassel row number 16 row, the row grain is several about 36, and fecundity is good; The seed pure white, glutinous sweet mating type contains 1/4 and strengthens sweet seed on the fruit ear.About thousand fresh weight 300g, bright seed-producing rate about 70%.Glutinous property purity is good, measures according to Agricultural College Affiliated to Yangzhou Univ., and amylopectin/total starch is 98.23%, and contrast Su Yu (glutinous) is for No. 1 98.24%.Exterior quality is 83.4 with tasting total points, and contrast Su Yu (glutinous) is for No. 1 82.9, and is close slightly excellent with contrast.Outward appearance and mouthfeel are all more excellent, should do the edible or quick-frozen processing variety utilization of fresh fruit fringe.
More anti-rough dwarf disease, incidence of disease 15-30%, contrast Suyunuo No.1 45-60%, and because the milpa that the present invention selects is high slightly, patience is stronger, majority still has commodity in the disease plant, is the stronger kind of rough dwarf disease patience.In addition, go back disease-resistanting leaf spot, banded sclerotial blight, stem rot etc., resistant to lodging, experimental planting test shows, contiguous provinces and cities spring and summer autumn sowing plantations such as suitable river, Zhejiang, Shanghai, Anhui.
Below in conjunction with embodiment the present invention is described further.
Embodiment
A kind of anti-rough dwarf disease is eaten sweet-waxy maizes single cross hybrid selection raw, comprises the following steps:
(1) selecting the waxy corn germplasm logical is 5 to form [logical is 5 * Shendan No.7 number white grain variant) logical be 5] breeding basic population, and by the continuous selfing of this breeding basic population, seed selection becomes the female parent of inbred line as crossbreed again; Said [logical is 5 * Shendan No.7 number white grain variant) logical be 5] breeding basic population be select for use in two generations of Shendan No.7 selfing white grain variant with logical be the crossbreed that No. 5 hybridization obtains, again with logical be the breeding basic population of backcrossing and obtaining for No. 5;
(2) select the waxy corn germplasm weigh white 522 with the high anti-germplasm T877 that contains tropical germplasm, in anti-germplasm general sweet 84, the three is hybridized in twos, each hybrid combination mixes by 50 seeds and forms original breeding populations, as the basic population of male parent choosing system;
(3) the good inbred line of seed selection: original breeding population is fully mixed powder, obtain mixing powder colony, chain to break; Then from mixing powder colony selfing 50 ~ 100 fringes; Selfing limit, limit is with non-assorted excellent crowd's inbred line test cross; Output (selecting to surpass more than 10%), resistance index (the selection field resistance is good) according to the test cross kind than the glutinous output of control group Su Yu; The final election major clique, and with the fully mixed powder generation of major clique breeding population; The continuous selfing of above-mentioned breeding population is produced inbred line make the male parent group;
(4) utilize three-step approach final election three recessive homozygote major cliques, with general sweet su, strengthen sweet se, glutinous wx three bottom recessive final elections, reservation:
The fruit ear of the segregative generation of the male parent group that the first step obtains from step (3) is selected glutinous grain; The choosing from the separation offspring of glutinous property seed of second step obtains sweet grain, in its sweet grain the sweet seed of general sweet and general sweet double reinforcement is arranged, and chooses earlier and does the slow person of not plump speed (slow approximately 1 day) in the air-dry seed, and preliminary choosing obtains the sweet seed of general sweet double reinforcement; Under the 3rd sweet seed kind of general sweet double reinforcement of step with primary election, bright fringe field harvest time adopt half fringe or 1/3 fringe to taste to confirm sugariness high be three recessive gene type homozygotes;
(5) adopt hybrid combination to identify the homozygotic MRDV resistance of above-mentioned three recessive gene types, select to have the male parent of the seed of MRDV resistance as crossbreed;
(6) female parent that step (1) is obtained becomes single cross hybrid with the paternal hybrid seed selection that step (5) obtains.
The method of the glutinous grain of first step selection is to select endosperm to be the seed of wax in the step (4).
The method of the sweet grain of second step selection is that the seed of selection profile shrinkage is sweet grain in the step (4).
Claims (3)
1. an anti-rough dwarf disease is eaten sweet-waxy maizes single cross hybrid selection raw, it is characterized in that: comprise the following steps:
(1) selecting the waxy corn germplasm logical is 5 to form [logical is 5 * Shendan No.7 number white grain variant) logical be 5] breeding basic population, and by the continuous selfing of this breeding basic population, seed selection becomes the female parent of inbred line as crossbreed again; Said [logical is 5 * Shendan No.7 number white grain variant) logical be 5] breeding basic population be select for use in two generations of Shendan No.7 selfing white grain variant with logical be the crossbreed that No. 5 hybridization obtains, again with logical be the breeding basic population of backcrossing and obtaining for No. 5;
(2) select the waxy corn germplasm weigh white 522 with the high anti-germplasm T877 that contains tropical germplasm, in anti-germplasm general sweet 84, the three is hybridized in twos, each hybrid combination mixes by the equivalent seed and forms original breeding population, as the basic population of male parent choosing system;
(3) the good inbred line of seed selection: original breeding population is fully mixed powder, obtain mixing powder colony, chain to break; Then from mixing powder colony selfing 50 ~ 100 fringes, selfing limit, limit is with non-assorted excellent crowd's inbred line test cross, according to output, the resistance index of test cross kind, and the final election major clique, and major clique fully mixed powder generation breeding population; The continuous selfing of above-mentioned breeding population is produced inbred line make the male parent group;
(4) utilize three-step approach final election three recessive homozygote major cliques:
The fruit ear of the segregative generation of the male parent group that the first step obtains from step (3) is selected glutinous grain; The choosing from the separation offspring of glutinous property seed of second step obtains sweet grain, in its sweet grain the sweet seed of general sweet and general sweet double reinforcement is arranged, and chooses earlier and does the slow person of not plump speed in the air-dry seed, and preliminary choosing obtains the sweet seed of general sweet double reinforcement; Under the 3rd sweet seed kind of general sweet double reinforcement of step with primary election, bright fringe field harvest time adopt half fringe or 1/3 fringe to taste to confirm sugariness high be three recessive gene type homozygotes;
(5) adopt hybrid combination to identify the homozygotic MRDV resistance of above-mentioned three recessive gene types, select to have the male parent of the seed of MRDV resistance as crossbreed;
(6) female parent that step (1) is obtained becomes single cross hybrid with the paternal hybrid seed selection that step (5) obtains.
2. anti-rough dwarf disease according to claim 1 is eaten sweet-waxy maizes single cross hybrid selection raw, it is characterized in that: the method for the glutinous grain of first step selection is to select endosperm to be the seed of wax in the step (4).
3. anti-rough dwarf disease according to claim 1 and 2 is eaten sweet-waxy maizes single cross hybrid selection raw, it is characterized in that: the method for the sweet grain of second step selection is that the seed of selection profile shrinkage is sweet grain in the step (4).
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986524A (en) * | 2012-12-17 | 2013-03-27 | 南京农业大学 | Breeding method for black and white sweet glutinous hybrid maize |
| CN103404430A (en) * | 2013-08-16 | 2013-11-27 | 广西壮族自治区农业科学院玉米研究所 | Method for breeding high-pro-vitamin A content maize inbred lines |
| CN105075856A (en) * | 2015-09-25 | 2015-11-25 | 云南省农业科学院粮食作物研究所 | Method for breeding double recessive homozygous sweet-waxy maize hybrid through sh2 gene type super-sweet maize mutant with high starch content |
| CN105284603A (en) * | 2015-12-01 | 2016-02-03 | 江苏沿江地区农业科学研究所 | Fresh maize heterosis group step-by-step classification germplasm improvement and breeding technology system |
| CN105379616A (en) * | 2015-12-01 | 2016-03-09 | 江苏沿江地区农业科学研究所 | Multi-ecological wide-adaptation multi-resistance and breeding-potentiality-incremental population improvement breeding system |
| CN106106156A (en) * | 2016-06-29 | 2016-11-16 | 无锡南理工科技发展有限公司 | A kind of breeding method of anti-rough dwarf disease Semen Maydis |
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| CN1411700A (en) * | 2002-04-08 | 2003-04-23 | 黄炳生 | Method of breeding sweet glutinous hybrid maize by utilizing sweet glutinous double recessive or triple recessive gene line |
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| CN1411700A (en) * | 2002-04-08 | 2003-04-23 | 黄炳生 | Method of breeding sweet glutinous hybrid maize by utilizing sweet glutinous double recessive or triple recessive gene line |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986524A (en) * | 2012-12-17 | 2013-03-27 | 南京农业大学 | Breeding method for black and white sweet glutinous hybrid maize |
| CN103404430A (en) * | 2013-08-16 | 2013-11-27 | 广西壮族自治区农业科学院玉米研究所 | Method for breeding high-pro-vitamin A content maize inbred lines |
| CN103404430B (en) * | 2013-08-16 | 2015-12-23 | 广西壮族自治区农业科学院玉米研究所 | A kind of method of cultivating high-pro-vitamin A corn inbred line |
| CN105075856A (en) * | 2015-09-25 | 2015-11-25 | 云南省农业科学院粮食作物研究所 | Method for breeding double recessive homozygous sweet-waxy maize hybrid through sh2 gene type super-sweet maize mutant with high starch content |
| CN105284603A (en) * | 2015-12-01 | 2016-02-03 | 江苏沿江地区农业科学研究所 | Fresh maize heterosis group step-by-step classification germplasm improvement and breeding technology system |
| CN105379616A (en) * | 2015-12-01 | 2016-03-09 | 江苏沿江地区农业科学研究所 | Multi-ecological wide-adaptation multi-resistance and breeding-potentiality-incremental population improvement breeding system |
| CN105284603B (en) * | 2015-12-01 | 2017-11-24 | 江苏沿江地区农业科学研究所 | The miscellaneous excellent group's step-by-step classifier Genetic improvement of fresh edible maize and breeding technique system |
| CN105379616B (en) * | 2015-12-01 | 2017-11-24 | 江苏沿江地区农业科学研究所 | More ecological Wide-adaptive multiresistance and the incremental population improvement breeding system of Breeding Potential |
| CN106106156A (en) * | 2016-06-29 | 2016-11-16 | 无锡南理工科技发展有限公司 | A kind of breeding method of anti-rough dwarf disease Semen Maydis |
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Application publication date: 20120627 |