CN105379616B - More ecological Wide-adaptive multiresistance and the incremental population improvement breeding system of Breeding Potential - Google Patents
More ecological Wide-adaptive multiresistance and the incremental population improvement breeding system of Breeding Potential Download PDFInfo
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- CN105379616B CN105379616B CN201510859952.3A CN201510859952A CN105379616B CN 105379616 B CN105379616 B CN 105379616B CN 201510859952 A CN201510859952 A CN 201510859952A CN 105379616 B CN105379616 B CN 105379616B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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Abstract
The invention discloses the incremental population improvement breeding system of a kind of how ecological Wide-adaptive multiresistance and Breeding Potential, including elite germplasm of three groups with recurrent selection initial population, resistance selection, Doubled haploid breeding, the test of DH systems, combining ability test, DH systems is selected to be applied to breeding and assemble the steps such as the second samsara initial population.The present invention advocates the recurrent selection for carrying out more pest and disease damage resistances, lodging resistance, yield etc. extensively under more ecological conditions, and carry out colony and exchange be combineding with each other, the more anti-, high yields of polymerization, the extensively excellent genes such as suitable, enable recurrent selection population hereditary basis constantly to widen, breeding performance is constantly lifted.
Description
Technical field
The present invention relates to the incremental population improvement breeding system of a kind of how ecological Wide-adaptive multiresistance and Breeding Potential.
Background technology
Corn is China's area and the first big crop of yield, and the big crop of the first in the world.China's corn per unit area yield is only at present
5416.7 kilograms/hectare（2008）, far below kilogram/hectare of the U.S. 10000（2005）.China's corn small area most high yield
Amount is up to 15000 kilograms/hectare or so, also far below kilogram/hectare of U.S.'s Yield contest 27750（U.S.'s Hawkeye State in 2002
Francis Childs create）.Over nearly one, 20 year, world's corn breeding trend is to improve the synthesis resistance of kind
To improve maize population yield, density is also being stepped up.Such as 20 th century America thirties, planting density less than 30000 plants/
hm2, yield 1200-2000kg/hm2Left and right;60-70 ages, density increase to 45000 plants/hm2, output increased to 3800-
5500 kg/hm2.Its planting density has increased to 67500 plants/hm at present2More than, output increased to 9000 kg/hm2More than；And its
The planting density in high-yield field is then up to 85500-109500 strains/hm2, yield reaches 15000 kg/hm2More than.The U.S. is in the world
The relatively early country with long-term development recurrent selection, the incomplete statistics only investigated according to the U.S. central and north, it improves colony for a long time
Type contains yield 44, yield and ripe phase 9, economical character 43, insect resistace 14, disease resistance 57, chemical composition
28, adaptability 15, the totally 246 population improvements work of other indefinite specific traits 36（Wherein use hybrid system method
60% is accounted for, other Reciprocal recurrent selections, half sisters, full sisters etc. account for 40%）, effectively promoted the development of Maize Production.Such as
BSSS（Iowa Jian Gan colonies）The population improvement up to more than 70 years is carried out, the Elite inbred such as seed selection B37, B73 is answered
For producing, the development of production has been promoted.At present, one of its population improvement Genetic improvement method still important as its.The U.S.
（Including America）The germplasm enhancement plan of new century, the major clique on the basis of breeding extensive work and cross combination before primarily resting on
Based on the method for inbred-variety cross of hybridization, although amount of increase in production is larger after an excellent germplasm is obtained, but the germplasm such as the torrid zone, subtropical zone has
The process of one domestication, its combining form are also similar to narrow basal profile, are difficult to carry out under the current breeding system in China.
MRDV is the disease for having a strong impact on China's Maize Production in recent years, and some local grave illness times even result in into
Ten thousand hectares of total crop failures.Domestic anti-rough dwarf disease germ plasm resource is poor at present, focuses mostly on and is utilizing 78599 germplasm.Stem rot is throughout the year tight
The disease of recurrence, grain reduces again after morbidity, plant lodging, serious threat mechanized harvest.Other such as gall smuts, to corn
Qualitative effects are larger；Dense planting is to improve one of most effective measure of yield, the resistance to close anti-fall and character that need to urgently improve at present.
The content of the invention
It is an object of the invention to provide the incremental group of a kind of good how ecological Wide-adaptive multiresistance of effect and Breeding Potential
The good breeding system of structural reform.
The present invention technical solution be：
A kind of how ecological Wide-adaptive multiresistance and the incremental population improvement breeding system of Breeding Potential, it is characterized in that：Including
The following steps：
（1）Three groups are selected to match somebody with somebody the elite germplasm of recurrent selection initial population；According to breeding pedigree and molecular labeling draw group according to
According to germplasm is divided into A, B, D tri- crowds：A female more fathers are respectively adopted in 3 monoid germplasm and mix powder hybridization, then plant this seed respectively
Three each 0.5 mu of isolated areas are implanted in, isolated area is not less than 500 meters with space length, or time isolation is not less than 20 days, obtains
Polling initial population；
（2）Resistance selects：Initial population is seeded under MRDV, stem rot susceptible disease environment, by susceptible strain emasculation
Or surgery superseded；
（3）Doubled haploid breeding：Using above three colony, in continent, autumn sowing or Hainan Island lure No. 1 to make male parent kind with height
Isolated area 1 is planted, area 1.5-2 mus, the seed for being pollen with induction is awarded after harvesting three mass emasculations, respectively in three monoids
By grain select with purple grain top, colourless plumule point, depression triangle embryo character quasi- monoploid seed；By three groups of quasi- monoploid
Seed spring or Hainan plantation Natural double；
Also can three groups of quasi- monoploid seeds plant and be unfavorable for haploid nature in adverse circumstance ecological condition, adverse circumstance ecological condition
Double, but be advantageous to the natural selection of haplobiont, easily obtain DH systems that are degeneration-resistant, extensively fitting, Natural double amount is 5~10%；
（4）DH systems test：Plant height, the Ear height of DH systems are recorded, pollination self phase, breeding time, is first sorted by plant height, from short
To height；Sorted again from morning to late pollination period or breeding time in short, middle and high bar, to register seed；The seed registered is planted in
Resistance to close lodging resistance is selected under 90000-120000 strains/ha environment, bar occurs for the pest and disease damage for being aided with the black powder of rough dwarf disease, stem rot, knurl
Part, select multiresistance；The measure of fast tester for water content progress dewatering speed, selection, record are also utilized simultaneously；
（5）Combining ability test：1 isolated area is planted after the DH systems registration of each two group, emasculation, utilizes 1 the 3rd group of generation
Table major clique is measured as Testers, is produced and is available for 3~9 resistance, agronomic characteristics, the seed of coordinate force test；Selection 3~9
The individual different pilot plantation of ecology, according to multi-point sampler result, choosing obtains more anti-, height and matches somebody with somebody, is dehydrated the excellent DH systems such as fast；Or choosing is obtained and had
Monoclonal antibody, height are matched somebody with somebody, are dehydrated fast, anti-fall extremely strong feature system；It is measured combined test density point density between 50000-90000 strains/ha
Carry out, identification traitses include 1,2 leaf angles, disease resistance on Yield and its components factor, lodging, fringe position, are tested and produced with major diagnostic
Measure coordinate force, thick contracting, stem rot, disease disease resistance, lodging resistance and the High planting of the black powder of knurl, the coordinate force of maturity period natural moisture content
Effect；
（6）DH systems are applied to breeding and assemble the second samsara initial population：Resistance and yield combining ability is excellent
DH systems directly apply to breeding combo；Simultaneously will distinctive elected DH systems in terms of most excellent and disease-resistant, anti-fall, coordinate force,
Carry out：1) phase mutual cross, the self-mating system germplasm of the multiple merits of selective polymerization is continued；2) formed newly after mixing powder by three monoids
Originally colony, newly originally it will mix and plant respectively in three isolated areas after colony's harvest, and obtain 3 the second samsara initial populations；3)
The colony after Breeding Potential is lifted, by DH breedings, all DH systems do not eliminate, while molecular labeling scanning is carried out, while entering
The thick contracting of row, the character multiple spot identification of stem rot, the pest and disease damage of the black powder of knurl, lodging resistance, dehydration property are related by the joint of repeatability
Analysis, carry out practicality Molecular Marker-assisted Selection and application；
（7）It will be selected via more ecotope Nature and Man works or the recurrence colony of recurrent selection, it is then abundant by isolated area
Mixed powder forms the colony that the Breeding Potentials such as new more anti-wide suitable yield get a promotion again.
Step（1）In three monoids assembling be that A monoids are synthesized with the PA group in corn breeding with Reid group's germplasm, B classes
Group is synthesized with PB with Lancaster germplasm, and D monoids are synthesized with Lucia Red Cob group with siping head group.
The classification of the present invention has the following advantages that with breeding technique method：
Three heap sort methods are adapted to China's actual conditions.The present invention does not follow the most important reason of the miscellaneous excellent patterns of external SS × non-SS
It is that the maximum Huang-Huai-Hai main maize area in China, its most suitable miscellaneous excellent pattern is A × D（Such as Zheng Dan 958）, or B × D（As Shandong is single
981）, the siping head blood lineage particularly in D group.And A × B（Such as first jade 335）It is North China, the most important suitable miscellaneous excellent mould in northeast
Formula, and the Lucia Red Cob blood lineage in D group is in Huang-Huai-Hai（Such as Yedan No.13）And east, North China can be advantageously used.
There is hybrid vigour between three groups, be easily bred as improved seeds.A, B, D of synthesis tri- crowds, both suited China's breeding reality,
Matched again with world's Breeding trend.A × D mode, the seed of single cross is represented as Zheng Dan 958（Once first-class National Scientific and Technological Progress Award is obtained）、
Yedan No.13 etc.；B × D mode, the seed of single cross is represented as red jade 13（Once first-class National Scientific and Technological Progress Award is obtained）, Shandong single 981 etc.；A × B moulds
Formula, the seed of single cross is represented as first jade 335（Year promotes more than 2,000,000 hectares）, Yi Dan 9 etc., while be also once american corn breeding
Most prevailing model.
Three heap sort methods are advantageous to absorb the external and holding excellent genetic resources in native country.The monoid of A, B, D tri- is synthesized, enters road wheel
Selection is returned, the new system for not only improving A, B group of the absorption relationship from foreign countries enters recurrent selection, is advantageous to keep China native country again
The intrinsic excellent characteristic of germplasm, moreover it is possible to ensure China through adapting to the excellent genetic lasting of a variety of ecotypes formation for a long time
Constantly penetrate into.Really, U.S.'s Major Maize band 37 ° of N~45 ° N of latitude, its ecological environment and China's corn great Qu Huang-Huai-Hais, China
North, northeast are substantially similar, thus determine the Reid, Lancaster, Aiwa's dent in the U.S. etc. in China's Maize Production great Qu
（Reid especially adapts to Huang-Huai-Hai, and Lancaster North China, northeast are more suitable）Strong adaptability, better resistance, yield are high.It is and Chinese
These great Qu adaptation germplasm siping head, Lucia Red Cob monoid major clique also penetrated into american corn band corn breeding plan it
In.The present invention advocates this germplasm quasi-group classification method, plays one and casts a brick to attract jade, if following can have worldwide germplasm innovation
Joint, might have a more scientific sorting technique, such as merge Lucia Red Cob type in PA, Reid monoid to form SS
Group, siping head is merged in PB, blue Card Type to form non-SS group, or other means, make full use of worldwide life
State adapts to and favorable variation.
Three heap sort methods are advantageous to the mutual improvement of major clique between group.Breeding practice shows, the formation of Heterosis of Maize Hybrid group
It is a dynamic process with development, for example PB monoids are selected from U.S.'s seed of single cross 78599, come from Reid germplasm and are divided into
There is hybrid vigour with Reid germplasm.According to incompletely statistics, the U.S. there are about 40 excellent seeds of single cross and isolate some major cliques and push away
Wide application, has developed into the excellent germplasm group for having hybrid vigour with Reid germplasm（Like 78599 groups, China）.This is simultaneously
Illustrate to be mainly derived from the Reid germplasm of the hard stalk collective species in Iowa, its hereditary basis is wide, descendant's general combining ability is high and suitable
Ying Xingqiang.Domestic multiple improved seeds are two groups of intermolecular hybrids, are then matched somebody with somebody with the 3rd group, such as agriculture China 101 and the Lushui River jade 13（It is long beautiful
13）, i.e.,（A×B）Self progeny again × D；Good beautiful 99, female parent is to be returned Zheng again using after first beautiful 335 cenospecies improvement Zheng 58
58, male parent is（Pellet 598 × prosperous 7-2）× prosperous 7-2 self progeny, cross combination [（A×B）× A] self progeny again ×
[(（D×B）× D) × D] self progeny, grand flat 206 is also similar, calabash shell serving as a dipper beautiful 35 is then（58 × Mo17 of Zheng）After the selfing of × Zheng 58
Dai Zaiyu G. eurycarpa combos；And middle list 909 to be then 58 × HD568 of Zheng be A with [（A×B）× D] self progeny's combo shape
Formula etc..
The present invention is advantageous to private kind enterprise and adds germplasm innovation system.The private kind enterprise major clique in the U.S. adds germplasm enhancement meter
Draw, need to will be discharged into that germplasm enhancement system is public, and public welfare department can after business self-mating system and the hybridization of the feature filtered out germplasm
Discharge the self-mating system selected.The ecological germplasm more than three groups of the present invention, is easy to the private enterprise to add this germplasm innovation system, simultaneously
Also it is easy to germplasm performance to improve internationalization, is also convenient for breeding man and Genetic improvement system is added in the case of germplasm origin is clear, make
Mass breeding potentiality are continually incremented or constantly widened Germplasms.
Combining ability test is passed through by the breeding of DH systems again, genetic distance between three groups can be widened, it is degeneration-resistant to be bred as more high yield
Improved seeds.
Improved by colony's samsara of 3 big monoids, be advantageous to excellent genes in the germplasm group in polymorphic type excellent genes storehouse
Accumulation reaches the purpose of Germplasms amplification with progressively polymerizeing；Choosing system afterwards is special with progressively improveing in propulsion simultaneously
It is not that a large amount of specific germplasm can be found in the yield combining ability and resistance test process of Doubled haploid line, then in same class
Character excellent genes germplasm out of the ordinary is selected in group polling offspring, progressively hybridization polymerization, reaches the multiple characters under similar genetic background
It is polygenic to be accumulated by generation, the breeding time limit is substantially shorter, realizes that the how anti-precocious dehydration of high yield waits multiple characters excellent genes soon
Polymerization.
It is purposefully added between this Genetic improvement planning authorities, mass breeding potentiality can be promoted to be incremented by, and order by signing an agreement
Vertical colony or feature germplasm exchange shared agreement.Via more ecotope Nature and Man works selection or recurrent selection recurrence colony,
Chain continuation natural selection and artificial selection are broken by the fully mixed powder of isolated area again, the how anti-eurytopicity yield of colony can be improved
Deng Breeding Potential.
With reference to embodiment, the invention will be further described.
Embodiment
A kind of how ecological Wide-adaptive multiresistance and the incremental population improvement breeding system of Breeding Potential, comprise the following steps：
（1）Determine germplasm：The elite germplasm of recurrent selection initial population is matched somebody with somebody by the group of final election three.
（2）Group's foundation is drawn according to breeding pedigree and molecular labeling, germplasm is divided into three groups：A monoids are with corn breeding
PA group synthesizes with Reid group's germplasm（Can be that to include the PAReid group such as Zheng 58, S951, N18S951, H991 be 15）, B monoids
Synthesized with PB with Lancaster germplasm（Can include S651, T877, YJ7, Shen 137, the blue card groups of the excellent PB of Mo17, SwanRU2
It is 18）, D monoids synthesize with Lucia Red Cob group with siping head group（Can be include pellet 340, E28, prosperous 7-2, comprehensive 3 prosperous 7-2,
414th, the major clique such as S44S 16）, a female more fathers are respectively adopted in 3 monoid germplasm and mix powder hybridization, are planted respectively after harvest each at three
0.5 mu of isolated area, isolated area are not less than 500 meters with space length, or time isolation is not less than 20 days, obtains polling initial population
Body.
（3）Resistance selects：Initial population is planted in the date of seeding that rough dwarf disease easily sends out by 90000 plants/ha or so density（Such as Jiangsu
Save after the April 15 sowed in spring to mid or late May, before June first arrival mid-June of summer sowing）, then be aided with the jointing stage in bearing desire and overflow
Fill once（It is allowed to dry after soaked 10 hours or is allowed to dry behind 1-2 days again soaked 10 hours）, grouting mid-term flood irrigation once（Soaked 10 is small
When after be allowed to dry or be allowed to dry behind 1-2 days again soaked 10 hours）To induce stem rot, eliminate folding strain, rough dwarf disease, stem rot
The serious strains of disease pest such as disease, the black powder of knurl；
（4）Dihaploid（DH）Breeding：Using above three colony, autumn sowing or Hainan Island in continent（Seed is larger to be beneficial to
Selection）No. 1 is lured to make male parent plantation isolated area 1 with height（1.5-2 mu）, harvest three mass emasculations after award with induce be pollen
Seed, select single times of the standard with characters such as purple grain top, colourless plumule point, depression triangle embryos by grain in three monoids respectively
Body seed；By three groups of quasi- monoploid seed spring or Hainan plantation Natural double [environmental benefits that weather is nice and cool, day and night temperature is big
In the Natural double of haplobiont；Adverse circumstance ecological condition（Thermal extremes, arid, low temperature and less sunshine, barren etc.）It is unfavorable for single times
The Natural double of body, but be advantageous to the natural selection of haplobiont, easily obtains degeneration-resistant, wide suitable DH systems, and general 5~10%, pole
Few germplasm doubles required artificial doubling below 3% or not）, or use colchicine（There is severe toxicity）+（Dimethyl alum（DMSO）, tween
80（Tween-80）With the usual auxiliaries such as the basic element of cell division）Seed soaking is doubled, or the herbicide first with certain concentration
Base amiprophos（APM, Amipro-phosmethyl）, propyzamide（Pronamide, Pronamide）, dinitroaniline（Fluorine
Happy spirit, Trifluralin）And oryzalin（Oryzalin）Deng herbicide, have in the leaf phase of monoploid seedling 5~7 to seedling sprinkling good
Good doubles effect." double the haploid method of corn in addition, China Agricultural University has been developed that using herbicide and special remove
Careless agent " patented technology（The patent No.：201110325133）It is available for utilizing.Obtain the DH systems seed that can be selfed.Due to monoploid quilt
Inductivity from Natural doubling rate because breeding basic material is different, and its haploid-induction of DH systems offspring with double rate can mention it is tired
Add lifting, therefore make base material with the material and excellent DH systems offspring for doubling rate using height induction and easily get twice the result with half the effort.
（5）DH systems test：Plant height, the Ear height of DH systems are recorded, pollination self phase, breeding time, is first sorted by plant height, from short
To height；Sorted again from morning to late pollination period or breeding time in short, middle and high bar, to register seed.The seed registered is planted in
Resistance to close lodging resistance is selected under 90000-120000 strains/ha environment, is aided with the pest and disease damages such as the black powder of rough dwarf disease, stem rot, knurl and bar occurs
Part, select multiresistance；Measure, the selection of dewatering speed etc. can also be carried out using auxiliary such as fast tester for water content simultaneously
Or record, the superior do self propagated more.
（6）Combining ability test：1 isolated area is planted after the DH systems registration of each two group, emasculation, utilizes 1 the 3rd group of generation
Table major clique is measured as Testers, is produced and is available for 3~9 resistance, agronomic characteristics, the seed of coordinate force test.Selection 3~9
The individual different pilot plantation of ecology, according to multi-point sampler result, choosing obtains more anti-, height and matches somebody with somebody, is dehydrated the excellent DH systems such as fast；Or choosing is obtained and had
Monoclonal antibody, height are matched somebody with somebody, are dehydrated fast, anti-fall extremely strong feature system.Be measured combined test density can divide between 50000-90000 strains/ha it is close
Degree is carried out, and identification traitses include Yield and its components factor, lodging（Root falls, stem fractures）, 1,2 leaf angles, disease resistance on fringe position（Slightly
Contracting, stem rot, the black powder of knurl, fringe corruption etc.）, with the disease disease resistance such as major diagnostic test yield combining ability, thick contracting, stem rot, the black powder of knurl,
The combining ability effect of lodging resistance and High planting, maturity period natural moisture content etc..
（7）DH systems are applied to breeding and assemble the second samsara initial population：Resistance and yield combining ability is excellent
DH systems directly apply to breeding combo；Simultaneously will distinctive elected DH systems in terms of most excellent and disease-resistant, anti-fall, coordinate force,
1) phase mutual cross, the self-mating system germplasm of the multiple merits of selective polymerization is continued；2) formed newly originally after mixing powder by three monoids
Colony, newly originally it will mix and plant respectively in three isolated areas after colony's harvest, and obtain 3 the second samsara initial populations.3) lifted
Colony after Breeding Potential, by DH breedings, all DH systems do not eliminate, while molecular labeling scanning is carried out, while carrying out thick
The character multiple spots such as the pest and disease damages such as contracting, stem rot, the black powder of knurl, lodging resistance, dehydration property are identified, are divided by the way that the joint of repeatability is related
Analysis, carry out practicality Molecular Marker-assisted Selection and application.
（8）By relational approaches such as production, teaching ＆ research cooperations, the division unit for being purposefully added the plan of this Genetic improvement is absorbed extensively,
By signing an agreement, promote mass breeding potentiality to be incremented by, and conclude colony and exchange agreement.Will be via more ecotope Nature and Man works
Selection or the recurrence colony of recurrent selection, then the Breeding Potentials such as new more anti-extensively suitable yield are formed again by the fully mixed powder of isolated area
The secondary colony to get a promotion.
Claims (1)
1. a kind of how ecological Wide-adaptive multiresistance and the incremental population improvement breeding method of Breeding Potential, it is characterized in that：Including under
Row step：
(1) three groups are selected to match somebody with somebody the elite germplasm of recurrent selection initial population；Group's foundation is drawn according to breeding pedigree and molecular labeling, will
Germplasm is divided into A, B, D tri- crowds：A female more fathers are respectively adopted in 3 monoid germplasm and mix powder hybridization, are then planted in this seed respectively
Three each 0.5 mu of isolated areas, isolated area are not less than 500 meters with space length, or time isolation is not less than 20 days, obtains polling
Initial population；
(2) resistance selects：Initial population is seeded under MRDV, stem rot susceptible disease environment, by susceptible strain emasculation or is cut
Except superseded；
(3) Doubled haploid breeding：Using above three colony, in continent autumn sowing or Hainan Island with height lure No. 1 make male parent plantation every
1 from area, area 1.5-2 mus harvest to award after three mass emasculations and lure the seed of No. 1 pollen with height, respectively in three monoids by
Grain select with purple grain top, colourless plumule point, depression triangle embryo character quasi- monoploid seed；By three groups of quasi- monoploid kinds
Sub- spring or Hainan plantation Natural double；
Or three groups of quasi- monoploid seeds are planted in adverse circumstance ecological condition, adverse circumstance ecological condition is unfavorable for haploid naturally adding
Times, but be advantageous to the natural selection of haplobiont, DH systems that are degeneration-resistant, extensively fitting easily are obtained, Natural double amount is 5~10%；
(4) DH systems test：Record plant height, the Ear height of DH systems, pollination self phase, breeding time, first sorted by plant height, from it is short to
It is high；Short, middle and high bar presses pollination period or breeding time again, is sorted from morning to evening, to register seed；The seed registered is planted in
Resistance to close lodging resistance is selected under 90000-120000 strains/ha environment, bar occurs for the pest and disease damage for being aided with the black powder of rough dwarf disease, stem rot, knurl
Part, select multiresistance；The measure of fast tester for water content progress dewatering speed, selection, record are also utilized simultaneously；
(5) combining ability test：1 isolated area is planted after the DH systems registration of each two group, emasculation, is represented using 1 the 3rd group excellent
System is measured as Testers, produces and is available for 3~9 resistance, agronomic characteristics, the seed of coordinate force test；Select 3~9 lifes
The different pilot plantation of state, according to multi-point sampler result, choosing obtains more anti-, height and matches somebody with somebody, is dehydrated fast excellent DH systems；Or choosing is obtained with single
It is anti-, high to match somebody with somebody, be dehydrated fast, anti-fall extremely strong feature system；Combined test density point density between 50000-90000 strains/ha is measured to enter
OK, identification traitses include 1,2 leaf angles, disease resistance on Yield and its components factor, lodging, fringe position, are coordinated with characterization test yield
Power, thick contracting, stem rot, disease disease resistance, lodging resistance and the High planting of the black powder of knurl, the combining ability effect of maturity period natural moisture content；
(6) DH systems are applied to breeding and assemble the second samsara initial population：By resistance and the excellent DH systems of yield combining ability
Directly apply to breeding combo；Simultaneously will distinctive elected DH systems, progress in terms of most excellent and disease-resistant, anti-fall, coordinate force：
1) phase mutual cross, the self-mating system germplasm of the multiple merits of selective polymerization is continued；2) new originally group is formed after mixing powder by three monoids
Body, newly originally it will mix and plant respectively in three isolated areas after colony's harvest, and obtain 3 the second samsara initial populations；3) lifting is educated
Colony after kind of potentiality, by DH breedings, all DH systems do not eliminate, and carry out molecular labeling scanning on one side, while progress slightly contract,
The character multiple spot identification of the pest and disease damage, lodging resistance, dehydration property of the black powder of stem rot, knurl, by the joint correlation analysis of repeatability, enters
Row practicality Molecular Marker-assisted Selection and application；
(7) it will be selected via more ecotope Nature and Man works or the recurrence colony of recurrent selection, then pass through the fully mixed powder of isolated area
Form the colony that the Breeding Potential of new more anti-wide suitable yield gets a promotion again；
Three monoids assembled in step (1) are that A monoids are synthesized with the PA group in corn breeding with Reid group's germplasm, B monoids with
PB is synthesized with Lancaster germplasm, and D monoids are synthesized with Lucia Red Cob group with siping head group.
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| CN105379616B true CN105379616B (en) | 2017-11-24 |
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| CN111837942B (en) * | 2020-08-19 | 2022-06-28 | 江苏沿江地区农业科学研究所 | Valence-by-valence incremental germplasm innovation method for corn resistance yield multi-trait combined improvement |
| CN116406614B (en) * | 2021-12-31 | 2024-07-30 | 北京市农林科学院 | Corn germplasm population construction and line selection method for rapid enrichment of multiple excellent genes |
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| CN102511382A (en) * | 2011-12-15 | 2012-06-27 | 江苏沿江地区农业科学研究所 | Method for breeding single cross hybrid of rough dwarf-resistant fresh sweet waxy maize |
| US8492620B1 (en) * | 2010-02-26 | 2013-07-23 | Syngenta Participations Ag | Variety corn line NPAA3676 |
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| US8492620B1 (en) * | 2010-02-26 | 2013-07-23 | Syngenta Participations Ag | Variety corn line NPAA3676 |
| CN102511382A (en) * | 2011-12-15 | 2012-06-27 | 江苏沿江地区农业科学研究所 | Method for breeding single cross hybrid of rough dwarf-resistant fresh sweet waxy maize |
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| Hybrid maize breeding with doubled haploids: III. Efficiency of early testing prior to doubled haploid production in two-stage selection for testcross performance;C. Friedrich H. Longin et.al.,;《Theor Appl Genet》;20070629;第115卷;第519-527页 * |
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