CN102726286A - Rapid backcross breeding method of super-sweet waxy corn selfing line - Google Patents
Rapid backcross breeding method of super-sweet waxy corn selfing line Download PDFInfo
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- CN102726286A CN102726286A CN201210184782XA CN201210184782A CN102726286A CN 102726286 A CN102726286 A CN 102726286A CN 201210184782X A CN201210184782X A CN 201210184782XA CN 201210184782 A CN201210184782 A CN 201210184782A CN 102726286 A CN102726286 A CN 102726286A
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Abstract
The invention discloses a rapid backcross breeding method of a super-sweet waxy corn selfing line. The method comprises the following steps: 1, preparing a three-way hybrid through treating a purple waxy corn elite selfing line JS0582 as a female parent and a super-sweet corn hybrid Jingtian No.3 as a male parent; 2, backcrossing through treating the JS0582 as the female parent and the three-way hybrid as the male parent; 3, selecting grains generated through the backcross in a selfing and backcrossing manner; 4, repeatedly step 3 five times; and 5, planting selected and remained 1 spikes, bagging, selfing, and selecting and remaining white super-sweet grains after maturation. 2-generation selfing is carried out, hereditary stable strain lines are selected, and a super-sweet waxy corn selfing line JS0681 is bred. The method solves a problem that how to rapidly introduce recessive super-sweet properties into the common waxy corn selfing line. According to the invention, recessive endosperm mutant gene is transferred through adopting the direct continuous backcross method, so near half of the breeding process is shortened, and the breeding efficiency is improved.
Description
Technical field
The present invention relates to a kind of quick backcross breeding method of ultra sweet-waxy maizes inbred line, belong to agriculture new variety selective breeding technology field.
Background technology
Waxy corn is also claimed waxy corn, and thicker wax shape endosperm is arranged in the seed.Its grain endosperm starch is amylopectin all almost, has higher viscosity.Several mineral materials nutritive elements such as waxy corn rich in proteins, several amino acids, calcium and phosphorus, nutritious, unique flavor, good palatability is easy to digest and assimilate, and its nutrition health-care functions has been increasing town and country consumer favor.
Along with improving constantly of the level of consumption, people also have higher requirement to the fresh edible maize edible quality, not only are satisfied with edible waxy corn, but hope to taste not only sweet but also glutinous specific type fresh edible maize.The seed selection of good sweet-waxy maizes inbred line is the basis of sweet-waxy maizes culturing cenospecies.
WxBe to influence the synthetic relevant gene of corn embryosperm amylopectin, the glutinous property that its recessive mutation proterties causes is widely used in the crossbreeded glutiuous maize seed selection.
Su,
Sh2,
BtDeng endosperm recessive gene mutant special separately gene effect is arranged all, relevant with the metabolism of corn embryosperm carbohydrate, show as saccharoid.Have
Sh2Genotypic corn is the wider super-sweet corn of a kind of present application, and milk ripe stage increases soluble sugar content greatly, and the ripe seed top depression is simply transparent.How apace the ultra sweet gene of recessiveness being imported to the key inbred line of existing core waxy corn, the ultra sweet-waxy maizes inbred line of seed selection is an a great problem.
Summary of the invention
Technical problem
The objective of the invention is provides a kind of method for fast the ultra sweet gene of recessiveness being imported in the key waxy corn inbred line, for cultivation high-quality, high ultra sweet-waxy maizes crossbreed anti-, high yield lay the first stone.
Technical scheme
1) be female parent with the key inbred line JS0582 of purple crlutinous maize core, the super-sweet corn crossbreed is a male parent brilliant sweet No. 3, is mixed with the basic material of triple hybrid as seed selection.
2) being maternal with JS0582, is that male parent is backcrossed with the triple hybrid, obtains the fruit ear of backcrossing.
3) seed of backcrossing adopts selfing+backcross method to select: i.e. seedling 10 strains and numbering are stayed in sowing arbitrarily, obtain 10 on selfing fruit ear after the selfing; Simultaneously be maternal with JS0582, the pollen of getting 10 strains respectively is that male parent is backcrossed and numbered, and 10 on the fruit ear of obtaining backcrossing divides the fringe threshing.
Separate the corresponding fruit ear of backcrossing of numbering with it of then selecting and remain if occur ultra sweet and normal seed on certain selfing fruit ear.
4) with 3), continue to adopt selfing+backcross method to backcross 5 times.
The fruit ear plantation back selfing of 5) selecting and remain, the wherein white ultra sweet seed of selecting and remain is promptly bred ultra sweet glutinous type corn inbred line.
The white ultra sweet seed of wherein selecting and remain after 2 generations of selfing, is selected the economical character head progeny row close with inbred line JS0582 again.
Separate if occur ultra sweet and normal seed in the step 3) on certain selfing fruit ear, the corresponding fruit ear of backcrossing of numbering with it of then selecting and remain can be chosen a fruit ear wantonly and be used for next step selfing+backcross method selection.
Beneficial effect
The present invention is based on the key waxy corn inbred line of existing core, and need be with the ultra sweet gene rapid transfer of recessiveness in key waxy corn inbred line.Because what use is that the key waxy corn inbred line of core is as recurrent parent; The ultra sweet-waxy maizes inbred line coordinate force of seed selection success, disease resistance, plant type etc. are similar with the recurrent parent height; Can test and direct assembling cross-fertilize seed without coordinate force, improve breeding efficiency.
The present invention is the recessive endosperm mutator gene of transformation, and the general procedure of breeding is to hybridize selfing more earlier to select recessive individual process, and what the present invention adopted is direct continuous backcross method, can shorten nearly half the breeding process, has improved breeding efficiency.
Because single recessive gene
Sh2Have when backcrossing 50% probability be heterozygous (
Sh2sh2), and 1-(50%)
10=1-0.0078125=0.9990, therefore in theory, 1 head progeny row only need be planted 10 strains in backcross process promptly has 99.9% probability to choose at least 1 to have the ultra sweet genotypic fruit ear of heterozygosis (Fig. 1).
Therefore the colony that uses of the present invention is less, and each is backcrossed in theory only needs plantation 10 strains can guarantee that 99.9% probability selects the individual plant that contains target gene from generation to generation, has dwindled the breeding scale greatly, practices thrift expenses such as human and material resources.
Because the ultra sweet seed on the selfing fruit ear shows as the clear-cut shrinkage of white, therefore very easily identification.
In addition, the present invention through the expansion can be widely used in as
Su,
Bt1,
Bt2,
Sh1Quick backcross breeding Deng single recessive gene.
Description of drawings
Fig. 1: the selfing+back-and-forth method of backcrossing diagram.
* explain that sweet and non-sweet separating appears in seed on arbitrary individual plant selfing fruit ear, then the corresponding fruit ear of backcrossing of numbering keeps optional wherein 1 fringe with it.For example, sweet and non-sweet the separation appear in seed on the selfing fruit ear of individual plant 2, and then the fruit ear of backcrossing of individual plant 2 keeps.If there is the more fruit ear of backcrossing to keep, then optional wherein 1 get final product.
Fig. 2: the application of the selfing+back-and-forth method of backcrossing in ultra sweet-waxy maizes inbred line breeding.
?
The practical implementation method
(Su Ke spends glutinous 2008 male parent to the key inbred line JS0582 of core, and Su Ke spends glutinous 2008 authorization bulletins: state examines beautiful 2008028; Zheng Zisong, Li Gang, Yuan Jianhua. Su Ke spends glutinous 2008 efficiency cultivation technology for high. and 2010, the Jilin vegetables, (2): 2-9.), brilliant sweet No. 3 commercially available getting.
1) be female parent with the key inbred line JS0582 of purple crlutinous maize core, brilliant sweet No. 3 is male parent, is mixed with the basic material of triple hybrid as seed selection.
2) being maternal with JS0582, is that male parent is backcrossed with the triple hybrid, obtains the fruit ear of backcrossing, and seed mixes and takes off.
3) backcross and mix seed plantation 1 district take off (stay seedling 10 strains and number), obtain 10 on selfing fruit ear after the selfing; Simultaneously be maternal with JS0582, the pollen of getting 10 strains respectively is that male parent is backcrossed and numbered, and 10 on the fruit ear of obtaining backcrossing divides the fringe threshing.
4) if occurring sweet and non-sweet seed on certain selfing fruit ear separates, the corresponding with it fruit ear of backcrossing of then selecting and remain, optional wherein 1 fruit ear.
5) with 3) and 4), continue to adopt selfing+backcross method to backcross 5 times.
The fruit ear plantation back bagging selfing of 6) selecting and remain, the white ultra sweet seed on the fruit ear of selecting and remain after the maturation is promptly bred ultra sweet glutinous type corn inbred line.
The ultra sweet glutinous type corn inbred line of being bred is successfully with the result of the ultra sweet gene rapid transfer of recessiveness to key waxy corn inbred line.Since use be key waxy corn inbred line as recurrent parent, the ultra sweet-waxy maizes inbred line coordinate force of seed selection success, disease resistance, plant type etc. are similar with the recurrent parent height, can be without the coordinate force test and direct assembling cross-fertilize seed, improve breeding efficiency.
7) the ultra sweet seed of white after 2 generations of selfing, is selected the economical character head progeny row close with inbred line JS0582 again, i.e. the ultra sweet glutinous type corn inbred line of inheritance stability is named and is ultra sweet glutinous type corn inbred line JS0681.This inbred line can be mixed to hand over and bred original seed through selfing under the Artificial Control or isolated area.The feature of inbred line JS0681: this inbred line is sowed in spring about 115 days in the area, Nanjing the time of infertility, and seedling first leaf sheath is green, and the rough leaf cochlear becomes strain leaf look dark green; Cane is green, and leaf margin is green, and the plant type hemicompact is gathered; 170 centimetres of plant heights, the fringe position is high 66 centimetres, 17 of total leaf numbers; The fringe upper blade is 5, and the tassel branch is flourishing, and pollen amount is big.Male flower lepicena base portion is green, and clever shell is green, and flower pesticide is green, and female Honoka silk is dark green, the short cartridge type of fruit ear, 15 ~ 17 centimetres of spike lengths, tassel row number 16 ~ 18 row, several about 22 of row grain.The grain white shrinkage, cob white.
Claims (3)
1. the quick backcross breeding method of a ultra sweet-waxy maizes inbred line is characterized in that following 7 steps:
1) be female parent with the key inbred line JS0582 of purple crlutinous maize, the super-sweet corn crossbreed is a male parent brilliant sweet No. 3, is mixed with the basic material of triple hybrid as seed selection;
2) being maternal with JS0582, is that male parent is backcrossed with the triple hybrid, obtains the fruit ear of backcrossing;
3) seed of backcrossing adopts selfing+backcross method to select:
The fruit ear seed of promptly backcrossing after planting stays seedling 10 strains and numbering arbitrarily, obtains 10 on selfing fruit ear after the selfing;
Simultaneously be maternal with JS0582, the pollen of getting 10 strains of above-mentioned numbering respectively is that male parent is backcrossed and numbered, and 10 on the fruit ear of obtaining backcrossing divides the fringe threshing;
Separate the corresponding fruit ear of backcrossing of numbering with it of then selecting and remain if occur ultra sweet and normal seed on certain selfing fruit ear;
4) with 3), continue to adopt selfing+backcross method to backcross 5 times;
The fruit ear bagging selfing after planting of backcrossing of 5) selecting and remain white ultra sweet seed, be the ultra sweet glutinous type corn inbred line of being bred.
2. method according to claim 1 is characterized in that: the white ultra sweet seed that the selfing of step 5) bagging is selected and remain, again 2 generations of selfing, select the economical character head progeny row close with inbred line JS0582.
3. quick backcross breeding method according to claim 1 and 2; It is characterized in that: separate if occur ultra sweet and normal seed in the step 3) on certain selfing fruit ear; Then select and remain and number the corresponding fruit ear of backcrossing with it, a therefrom optional fruit ear is used for next step selfing+backcross method and selects.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986524A (en) * | 2012-12-17 | 2013-03-27 | 南京农业大学 | Breeding method for black and white sweet glutinous hybrid maize |
| CN105075856A (en) * | 2015-09-25 | 2015-11-25 | 云南省农业科学院粮食作物研究所 | Method for breeding double recessive homozygous sweet-waxy maize hybrid through sh2 gene type super-sweet maize mutant with high starch content |
| CN108935085A (en) * | 2018-07-20 | 2018-12-07 | 江苏省农业科学院 | A kind of selection of black sweet-waxy maizes cenospecies |
| CN110150135A (en) * | 2019-05-05 | 2019-08-23 | 海南绿川种苗有限公司 | A kind of corn breeding method of sugar glutinous fresh-eating |
| CN114568302A (en) * | 2022-04-01 | 2022-06-03 | 海南绿川种苗有限公司 | Breeding method of sweet waxy corn |
| CN115226627A (en) * | 2022-09-26 | 2022-10-25 | 北京市农林科学院 | Method for efficiently creating sweet waxy corn inbred line |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108935085A (en) * | 2018-07-20 | 2018-12-07 | 江苏省农业科学院 | A kind of selection of black sweet-waxy maizes cenospecies |
| CN108935085B (en) * | 2018-07-20 | 2021-11-19 | 江苏省农业科学院 | Breeding method of black sweet waxy corn hybrid |
| CN110150135A (en) * | 2019-05-05 | 2019-08-23 | 海南绿川种苗有限公司 | A kind of corn breeding method of sugar glutinous fresh-eating |
| CN114568302A (en) * | 2022-04-01 | 2022-06-03 | 海南绿川种苗有限公司 | Breeding method of sweet waxy corn |
| CN115226627A (en) * | 2022-09-26 | 2022-10-25 | 北京市农林科学院 | Method for efficiently creating sweet waxy corn inbred line |
| CN115226627B (en) * | 2022-09-26 | 2022-12-27 | 北京市农林科学院 | Method for creating sweet waxy corn inbred line and application |
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Effective date of registration: 20190304 Address after: 210014 5th Floor, Comprehensive Building, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Xuanwu District, Nanjing City, Jiangsu Province Patentee after: Jiangsu Agricultural Science and Technology Transfer Center Co., Ltd. Address before: 210014 No. 50 Zhongling Street, Xuanwu District, Nanjing City, Jiangsu Province Patentee before: Jiangsu Academy of Agricultural Sciences |
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