In recent years, because China has solved problem of food and clothing on the whole substantially, people's living standard improves constantly, and is more and more exquisite to the taste and the healthy nutritive value of food.Ever-increasing in order to satisfy people to high-quality, the demand of diversification food, the quality of improving the people's livelihood and eating habit, make agricultural production, especially land utilization efficiency is stablized, continue, develop in a healthy way, the adjustment of pattern of farming is being carried out in China's agricultural production, the cultivated area of special corn is increasing, sweet-waxy maizes particularly, utilize the fresh fruit fringe owing to pluck, southern area can be planted 2-4 season in 1 year, and economic benefit obviously improves, and one season of sweet-waxy maizes of one mu of moderate-yield can be received 750 kilograms of fresh fruit fringes, about thousand yuan of net incomes, exceed 4 times than the plantation conventional corn.But the sweet-waxy maizes kind is mainly white, yellow at present, and mouthfeel is uneven.Because the corn hybrid seed plant is genotype heterozygosis homogeneity (F from generation to generation
1), but the seed of tying belongs to F
2In generation, genotype occur to be separated, if therefore crossbreed father, this genotype of mother are inconsistent, then the seed of being tied on the crossbreed plant is occurred separating by the proterties of the Gene Handling of embryo own.The edible quality of corn ear is a comprehensive proterties, and not exclusively by the decision of plant gene, the proterties of some effects taste is by the gene decision of seed embryo itself.For guarantee on the crossbreed plant institute as a result the edible quality of fringe be consistent, the part edible quality proterties of crossbreed father, this inbred line of mother must be identical.At home and abroad there is no the report that utilizes the high-quality sweet glutinous aubergine hybrid maize of aubergine sweet-waxy maizes recurrent selection population seed selection.
The objective of the invention is to produce the single present situation of hybridized sweet and glutinous corn combination that goes up application at present, the breeding method of the new combination of a kind of seed selection hybridized sweet and glutinous corn is provided, breed high-quality aubergine sweet-waxy maizes crossbreed at China.
Technical scheme provided by the present invention is at first introducing the sweet corn of domestic and international different regions, super-sweet corn, waxy corn, coloured corn, the how anti-ordinary maize of high yield, resources such as high-combining ability ordinary maize inbred line germplasm, comprise Suyunuo No.1, U.S.'s super-sweet corn, bright No. 1 of Tianjin, Yedan No.13 number and tuck in single No. 4 crossbreed, 478, yellow morning four, the U8112 inbred line, Shuyang waxy corn landrace, granary waxy corn landrace, set up basic population through hybridization and mutual the friendship, make all genes of each starting material enter basic population, guarantee the genetic diversity of basic population by same ratio.Then seed redness, glutinous property, sweet taste proterties are carried out repeatedly recurrent selection, form aubergine sweet-waxy maizes recurrent selection population.The bright fringe part of corn edible quality proterties stable and consistent in this colony, but economical character is variant.Obtain a series of inbred lines through inbreeding of more generation, inbred line is identified the product ratio through combining ability test and hybrid combination, select plant type compactness, seed wine-colored sweet-waxy maizes inbred line 96-6 and plant type is loose, pollen amount is big, seed light red, the splendid sweet-waxy maizes inbred line 96-6 of edible quality, do father, mother respectively originally with inbred line 96-5 and inbred line 96-6, high-quality aubergine sweet-waxy maizes crossbreed Nannongziyunuo No.1 is bred in hybridization.
Have the required proterties of high edible quality and yielding ability, yield stability, the strong required proterties of adaptability owing to build up the selected germplasm of basic population, and derive from domestic and international different regions, guaranteed the genetic diversity of basic population.Seed redness, glutinous property, sweet taste proterties are carried out many aubergine sweet-waxy maizes recurrent selection populations for recurrent selection formation, and it is good and consistent to have edible quality, and economical character has more rich variation.Carry out combining ability test and hybrid combination evaluation product ratio to selfing is many for the inbred line that obtains, filter out the aubergine sweet-waxy maizes inbred line that economical character is better, comprehensive resistance good, adaptability is strong, coordinate force is high.Hybridize again between inbred line and just obtain high-quality aubergine hybridized sweet and glutinous corn combination easily.Below on the south agricultural Qarnet narrate advantage of the present invention and good effect for example glutinous No. 1:
1. exterior quality and edible quality are good.Nannongziyunuo No.1 presentation quality and edible quality proterties that application the inventive method is bred are better than contrasting Suyunuo No.1.Suyunuo No.1 seed color is a white, and glutinous property and sugariness are medium; Nannongziyunuo No.1 seed color is a peony, and bright fringe drum grain is after to boil color and luster vivid, the sugariness height, and it is long that sugariness is kept the phase, and stamen is tender and sweet, and stamen is thin, seed-producing rate height, slightly fragrance.Nannongziyunuo No.1 another one distinguishing feature is bright fringe drum grain kernel seed coat colour white in early stage, and sugariness is higher; Mid-term, the kernel seed coat colour light red was not only sweet but also fragrant; Later stage kernel seed coat colour aubergine, sweet taste descends, fragrance and the enhancing of glutinous property.Therefore, can select suitable harvesting period according to the food custom of different regions.
2. exterior quality and edible quality stable and consistent.Because Nannongziyunuo No.1 two parental inbred line 96-5,96-6 all are that seed selection forms from aubergine sweet-waxy maizes recurrent selection population, therefore by bright fringe exterior quality and the edible quality proterties stable and consistent of inbred line 96-5 with the crossbreed of 96-6 hybrid seeding generation, plant experimentally through multiple years and to show, the coefficient of variation of Nannongziyunuo No.1 seed color, glutinous property, sweet taste proterties is 0-0.5%, almost do not separate, its stability is better than contrasting Suyunuo No.1.
3. Other Main Agronomic Characters is good, shows as high yield, stable yields.Because building up the selected germplasm of basic population has comprised how anti-ordinary maize of part high yield and high-combining ability ordinary maize inbred line, two inbred line 96-5 that selected in aubergine sweet-waxy maizes recurrent selection population that economical character is better, comprehensive resistance is better, coordinate force is higher and 96-6 have guaranteed the high yield and the stable yields characteristic of Nannongziyunuo No.1 as the parent of aubergine hybridized sweet and glutinous corn Nannongziyunuo No.1.Nannongziyunuo No.1 is sowed in spring 105 days breeding times in Nanjing area, and 88 days breeding times of summer sowing, plant is hung down loosely, and has to tiller and many fringes phenomenon, and the field comprehensive resistance is stronger.The fruit ear diameter is thin, and Dan Xiansui weighs about 130 grams, and the seed marshalling is full complete, uniformity, and quality is tight, and is no bald.About 600 kilograms of the bright fringes of per mu yield.Participated in Jiangsu Province's special corn regional test in 1999, bright fringe per mu yield is a little less than the contrast Suyunuo No.1, and difference is not remarkable, but exterior quality and edible quality are better than Suyunuo No.1.(seeing Table 1)
4. wide adaptability.Owing to build up the selected germplasm origin of basic population in domestic and international different regions, also comprised the adaptable ordinary maize kind of part, guaranteed to select the Nannongziyunuo No.1 of wide adaptability.Nannongziyunuo No.1 has been generalized to 20 provinces and cities such as Jilin, Hebei, Henan, Hubei, Fujian, Guangxi, Shanxi, Heilungkiang, Hainan, Yunnan, Hunan, Jiangxi, Anhui, Shandong, Zhejiang, Jiangsu, Beijing, Shanghai at present, plants experimentally in Spain in 99 years.
5. male parent inbred line pollen amount is big.The inbred line 96-5 flower pesticide of breeding with the inventive method is big, and pollen amount is many, and maternal 96-6 economical character is good, the grain yield height.Production of hybrid seeds test shows Nannongziyunuo No.1 seed farm ripening rate height, and the per mu yield of spring system has guaranteed that up to the 200-250 kilogram breeding cost is lower, helps a large amount of production of hybrid seeds and large tracts of land and promotes.Table 1 Other Main Agronomic Characters comparative test result (experiment station, Agricultural University Of Nanjing Jiangpu in 1999)
Kind (being) | The sowing to take out hero (my god) | Bright fringe breeding time (my god) | Plant height (cm) | The seed color | Bright spike length degree (cm) | Bald sharp length (cm) | Bright fringe heavy (g) | Hundred fresh weights (g) | Bright fringe output (kg/ mu) |
Nannongziyunuo No.1 | ????68 | ????88 | ????215 | Dark red | ????15.7 | ????0.3 | ????129 | ????27.7 | ????594.8 |
Suyunuo No.1 | ????64 | ????85 | ????210 | In vain | ????15.9 | ????0.4 | ????124 | ????23.3 | ????608.1 |
Inbred line 95-5 | ????71 | ????90 | ????160 | Pale red | ????14.4 | ????0.2 | ????115 | ????21.5 | ????311.8 |
Inbred line 96-6 | ????68 | ????87 | ????170 | Dark red | ????15.2 | ????0.8 | ????137 | ????27.6 | ????381.5 |
Annotate: P
1, P
2..., P
32It is respectively the original kind code name of setting up basic population.Use the concrete steps of the inventive method seed selection Nannongziyunuo No.1:
First step work: set up basic population.By breeding objective (seed selection high-quality, high yield, stable yields, good, the adaptable aubergine hybridized sweet and glutinous corn of comprehensive resistance) select to have high edible quality proterties and yielding ability, yield stability, the germ plasm resource Suyunuo No.1 of adaptable domestic and international different regions, U.S.'s super-sweet corn, bright No. 1 of Tianjin, Yedan No.13 number and tuck in single No. 4 crossbreed, 478, yellow early four, U8112 inbred line, Shuyang waxy corn landrace, granary waxy corn landrace, through hybridization with hand over mutually and set up basic population with each all gene of starting material.
Second step work: set up aubergine sweet-waxy maizes recurrent selection population.In basic population, seed redness, glutinous property, sweet taste proterties are carried out the recurrent selection of 5 generations, form that bright fringe exterior quality is consistent with the part edible quality, the separative aubergine sweet-waxy maizes of economical character recurrent selection population.
The 3rd step work: seed selection aubergine sweet-waxy maizes inbred line.In aubergine sweet-waxy maizes recurrent selection population, obtain a series of inbred lines through inbreeding of more generation, identify the product ratio through combining ability test and hybrid combination, filter out economical character is better, comprehensive resistance good, adaptability is strong, coordinate force is high aubergine sweet-waxy maizes inbred line 95-5 and 95-6, obtain Nannongziyunuo No.1.
The 4th step work: inbred line 96-5 and inbred line 96-6 original seed be self propagated respectively, makes father, this hybrid seeding of mother respectively with inbred line 96-5 and inbred line 96-6.