CN107581055A - A kind of breeding method of blue or green stalk preserved egg dish - Google Patents
A kind of breeding method of blue or green stalk preserved egg dish Download PDFInfo
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- CN107581055A CN107581055A CN201710754227.9A CN201710754227A CN107581055A CN 107581055 A CN107581055 A CN 107581055A CN 201710754227 A CN201710754227 A CN 201710754227A CN 107581055 A CN107581055 A CN 107581055A
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Abstract
The invention discloses a kind of breeding method of blue or green stalk preserved egg dish, comprise the following steps：（1）Different types of preserved egg dish resource is collected, carries out florescence selfing, self-compatable index is chosen and is less than 2.0, the not affine individual plant of selfing that bouquet is loose, bennet is dark green；（2）Continue to choose the individual plant for meeting objective trait in not affine individual plant is selfed, stripping flower bud was selfed for 56 generations, chooses florescence self-compatable index and is less than 0.5, flower bud phase self-compatable index is more than 10.0 stable self incompatible line；（3）Two complementary parents of inhereditary feature are chosen in self incompatible line is stablized, reciprocal cross is carried out and flower is pollinated, while choose positive and negative two cross combination that florescence hybridization index of affinity is more than 8.0, are reserved seed for planting.The double sowings all prepared using two parents for self incompatible line, genetic complementarity is high, and heterosis, hybrid vigor is obvious, strong adaptability, and two parents, which produce seed, can harvest utilization, can adapt to the different market demands simultaneously.
Description
Technical field
The present invention relates to a kind of selection of preserved egg dish, and in particular to a kind of breeding method of blue or green stalk preserved egg dish, belongs to
Field of plant variety breeding technology.
Background technology
In recent years, bouquet loose type cauliflower（Abbreviation preserved egg dish, similarly hereinafter）Won with the quality of its light green bennet, crisp sweet tea
Liking for consumer, popular city is popular rapidly at home, compared with the more close bouquet of general cauliflower, preserved egg cauliflower
Ball is loose, bennet is long, bennet is green, sweet tea of tasting, crisp, tender.When stir-frying and eating and cooking, it is not easy rotten, bennet color becomes more after heating
Green, condition is good；During dehydration processing, based on edible bennet, stalk is grown, and stalk color is dark green.By to preserved egg dish Main Nutrient Quality
Analysis, the μ gg of chlorophyll content 12.8-1(FW), the μ gg of carotenoid content 3.3-1(FW), VC contents 629mgkg-1 Can
Dissolubility sugared content 2.82%, compared with close type cauliflower, chlorophyll content wants high 2.7 times, and carotenoid content is high by 1.5
Times, soluble sugar is higher by nearly 10%.Preserved egg dish is most early in Fujian and Taiwan plantation consumption, in recent years from Fujian Zhejiang area
Develop into China rapidly.At present, the main production distribution of China's preserved egg dish is in the middle and lower reach of Yangtze River, Fujian, Zhejiang, Hubei Province alpine region,
Lanzhou, Bashang Grassland area, and North China, the extensive Relatively centralized again of planting area, have basically formed the confession of preserved egg dish anniversary
The production pattern in market is answered, cultivated area is up to 20,000 hm2More than, the 10% of cauliflower production area is accounted for, with consumer's
Accreditation receives, and preserved egg dish will plant extensively at home, and one of main Types as cauliflower consumption, and cultivated area will be up to 10
Ten thousand hm2More than, development potentiality is huge.
Preserved egg dish belongs to cross pollinated plant, heterosis, hybrid vigor performance is obvious, commodity, resists as cruciate flower class crop
Characteristic of disease, adaptability etc. shows obvious heterosis, hybrid vigor, but existing breeding technique is by a self incompatible line（It is female
This）With a self-mating system（Male parent）Plant in proportion, so as to harvest the seed produced on self incompatible line, and male parent is typically awarded
Powder is pulled out after terminating, and so causes seed production relatively low, land wastage, cost increase.And utilize two self incompatible line parents
The advantages that this difference preparing hybrid kind simultaneously, has that hybridization is easy, time and labour saving, inheritance stability, and seed production is high, and can be with
Abundant excellent genes performance, once harvests two distinct types of cenospecies, application prospect is wide in terms of preserved egg dish crossbreeding
It is wealthy.
The content of the invention
The technical problem of solution：For the deficiency of existing preserved egg dish crossbreeding technology, the invention provides a kind of blue or green stalk
The breeding method of preserved egg dish, using two self incompatible line parents to flower pollination hybridization, while select two different types
Preserved egg vegetable kind.
Technical scheme：A kind of breeding method of blue or green stalk preserved egg dish provided by the invention, comprises the following steps：
（1）Different types of preserved egg dish resource is collected, the observation of plant character within whole breeding time, evaluation resource is given birth to ripe
Property, adaptability, yielding ability, commodity, disease resistance, specificity, screen fine individual plant, carry out shelling the selfing of flower bud bagging at the florescence, mirror
Its fixed self-incompatibility, choose the not affine individual plant of selfing that self-compatable index is less than 2.0；
（2）In step（1）In the not affine individual plant of selfing of the self-compatable index of screening less than 2.0, continuing selection has target
The individual plant of character, in stripping flower bud bagging selfing 5-6 generations at florescence, choose florescence self-compatable index and be less than 0.5, flower bud phase self-compatible refers to
Stable self incompatible line of the number more than 10.0；
（3）In step（2）Two complementary parents of Multi-Objective Genetic character are chosen in the stable self incompatible line of gained, are carried out just
Reciprocal cross is pollinated to flower, identifies the hybridization affinity of positive and negative cenospecies respectively, while is chosen florescence hybridization index of affinity and be more than 8.0
Positive and negative two cross combination, reserves seed for planting.
Step（1）The fine individual plant is the individual plant that bouquet is loose, bennet is green, yield is high.Step（1）The florescence stripping
Flower bud bagging is selfed mode：Colored full bud can be opened after peelling off 3 days, exposes column cap, adopts the flower of morning on same day opening, often
Piece flower pollination 5 column caps, utilize parchment pollination bag bagging isolation.
Step（2）The objective trait individual plant is more than 23, blade, bennet green, growing way is strong, adaptability is good, yielding ability
Individual plant good, disease resistance is strong.
Step（3）The objective trait of one parent is：Growing way is strong, and siphonal lobe more than 23, bouquet is pure white, ball-type week
Just, bouquet surfacing, bennet green, comprehensive disease resistance is good, and yield is high, and Agronomic trait inheritance stability, development of floral organs is just
Often, nectary is flourishing；The objective trait of another parent is：Plant shape is upright, and siphonal lobe more than 23, bouquet is pure white, bennet length
And it is green, internal lobe is elongated, and comprehensive disease resistance is good, and Agronomic trait is stable, and development of floral organs is normal, and nectary is flourishing.
Beneficial effect：（1）On the premise of the current not a large amount of transformations of preserved egg dish resource are into sterile line, carried by inbreeding of more generation
It is pure, substantial amounts of different types of Agronomic trait stabilization is obtained, adaptability is good, and disease resistance is strong, the good good resource of yielding ability, special
It is not self incompatible line resource.
（2）Positive and negative two cenospecies are prepared simultaneously using the positive and negative hybridization of two self incompatible line parents, inhereditary feature is mutual
To mend, heterosis, hybrid vigor is obvious, strong adaptability, and two parents, which produce seed, can harvest utilization, can meet the different market demands simultaneously,
Yield increases nearly 1 times than the seed production way of single self incompatible line, economizes the land resource, time and labour saving, and the market competitiveness is strong.
Brief description of the drawings
Fig. 1 is the flow chart of the blue or green stalk preserved egg dish breeding method of the embodiment of the present invention 1.
Embodiment
Technical scheme is described further below by the mode of embodiment, but the protection domain invented is simultaneously
Therefore following embodiments are not confined to, but are limited by description and claims of this specification.
Embodiment 1
1）According to the maritime regional preserved egg food market demand in Yunnan Yuxi（Plant type is upright, and bouquet is straight, green grass or young crops stalk, breeding time 65-75d,
More summer ability is strong, and disease resistance is strong）With Xuzhou preserved egg food market demand（Plant growing way is vigorous, and bouquet is straight, surfacing,
Bennet is long and green, breeding time 75-85d, and disease resistance is strong）, start the 51 portions of preserved egg dishes that will have been collected since 1997 in July, 2004
Resource carries out field observation, identification, and the first generation is selfed not affine identification, filters out selfing not list of the index of affinity less than 2
Strain, and individual plant is reserved seed for planting.
2) 2005 by the not affine individual plant of the selfing filtered out, according to objective trait, according to the respective plant type of material, and ball-type,
Bennet color, Peduncle Length, disease resistance, selfing not index of affinity, floral organ and nectary be oriented screening, finally from Japan with
Two plants of selfings for possessing required objective trait not affinitive material is filtered out in the preserved egg vegetable kind of TaiWan, China respectively.
3）By filter out two selfings, affinitive material is not carried out according to objective trait, separates, carried within 2006 to 2009
Pure, individual plant is reserved seed for planting, and is selfed the not affine lasting progress of identification.
By constantly purifying, two parts of stable materials 001-1-1 and 1123-2 of two characters are obtained in April, 2010.Its
In, its feature of 001-1-1 is：Growing way is vigorous, and bouquet is oblate, and ball-type is straight, and bouquet surface is smooth, and green grass or young crops stalk, disease resistance is good, puts down
Single ball weight 1200g, breeding time 80d；Another its feature of parent 1123-2 is：Erect plants, bouquet is pure white, bouquet taper, flower
Obstruct long and green, disease resistance is good, average single ball weight 800g, breeding time 75d.
4）In December, 2010,001-1-1 and 1123-2 is subjected to reciprocal cross to flower pollination trial at the florescence, identifies two parents
This hybridization affinity, it is found that two material reciprocal crosss can normally set seeds, and seed is normal, full.Wherein, 001-1-1 is mother
This numbering is that 1405,1123-2 is that maternal numbering is 1422.
5）1405 and 1422 are arranged within 2011 to carry out regional test in Yunnan Province's Yuxi and Jurong City of Jiangsu Province.Its
In, Yunnan Province sets two dates of seeding, May 15 sowing time and August 10 days, and resident time is respectively June 13 and September 11 days；
Jiangsu Province sets two dates of seeding, and the sowing time is respectively June 15 and July 13, and resident time is respectively July 13 and August
13 days.During experiment, spacing in the rows 50cm, line-spacing 50cm are colonized, other field management are normally carried out by local planting habit.Regionality examination
It is as shown in table 1 to test investigation result：
Table 1
6）By regional test, the survey showed that, 1405 Yunnan date of seeding in May and August date of seeding plant type, ball-type it is straight, fertility
Phase, yield, disease resistance, all accord with the demands of the market, accorded with the demands of the market in Jiangsu date of seeding in June, date of seeding in July due to later stage low temperature,
Risk be present；1522 because bennet is grown, and ball-type is taper, is not yet received by Yunnan market, its plant type, ball-type, life in Jiangsu
Educate phase, yield and disease resistance and all meet high-end market demand, and Planting risk be present due to later stage low temperature in sowing in July.
7）It is final to determine that the regional demonstration that numerous double sowings carry out next step is expanded in production by regional test result,
In July, 2012 carries out the breeding processed work of double sowings in Yunnan Province's Yuanmou County, using hole plate seedling growth, according to 001-1-1 and 1123-
2 breeding time carries out intermission seeding, and controlling the mode for cutting the ball time to adjust, full-bloom stage is consistent, and multiplication fieldses processed are in florescence previous star
Every mu of phase places 1-2 casees honeybee and carries out supple-mentary pollination, and more than periphery 1500m does not have other kind cauliflower, broccoli and wild cabbages
Class vegetables seed farm.
Claims (5)
1. a kind of breeding method of blue or green stalk preserved egg dish, it is characterised in that comprise the following steps：
（1）Different types of preserved egg dish resource is collected, the observation of plant character within whole breeding time, evaluation resource is given birth to ripe
Property, adaptability, yielding ability, commodity, disease resistance, specificity, screen fine individual plant, carry out shelling the selfing of flower bud bagging at the florescence, mirror
Its fixed self-incompatibility, choose the not affine individual plant of selfing that self-compatable index is less than 2.0；
（2）In step（1）In the not affine individual plant of selfing of the self-compatable index of screening less than 2.0, continuing selection has target
The individual plant of character, in stripping flower bud bagging selfing 5-6 generations at florescence, choose florescence self-compatable index and be less than 0.5, flower bud phase self-compatible refers to
Stable self incompatible line of the number more than 10.0；
（3）In step（2）Two complementary parents of Multi-Objective Genetic character are chosen in the stable self incompatible line of gained, are carried out just
Reciprocal cross is pollinated to flower, identifies the hybridization affinity of positive and negative cenospecies respectively, while is chosen florescence hybridization index of affinity and be more than 8.0
Positive and negative two cross combination, reserves seed for planting.
A kind of 2. breeding method of blue or green stalk preserved egg dish according to claim 1, it is characterised in that step（1）It is described excellent
Individual plant is the individual plant that bouquet is loose, bennet is green, yield is high.
A kind of 3. breeding method of blue or green stalk preserved egg dish according to claim 1, it is characterised in that step（1）The florescence
Stripping flower bud bagging is selfed mode：Colored full bud can be opened after peelling off 3 days, exposes column cap, adopts the flower of morning on same day opening,
Every flower 5 column caps of pollination, utilize parchment pollination bag bagging isolation.
A kind of 4. breeding method of blue or green stalk preserved egg dish according to claim 1, it is characterised in that step（2）The target
Character individual plant is more than 23, blade, bennet green, the individual plant that growing way is strong, adaptability is good, yielding ability is good, disease resistance is strong.
A kind of 5. breeding method of blue or green stalk preserved egg dish according to claim 1, it is characterised in that step（3）It is one
The objective trait of parent is：Growing way is strong, and siphonal lobe more than 23, bouquet is pure white, and ball-type is straight, bouquet surfacing, bennet green,
Comprehensive disease resistance is good, and yield is high, and Agronomic trait inheritance stability, development of floral organs is normal, and nectary is flourishing；Another described parent
Objective trait be：Plant shape is upright, and siphonal lobe more than 23, bouquet is pure white, and bennet is long and green, and internal lobe is elongated, and comprehensive disease resistance is good,
Agronomic trait is stable, and development of floral organs is normal, and nectary is flourishing.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113287471A (en) * | 2021-04-22 | 2021-08-24 | 云南省农业科学院热区生态农业研究所 | Stock breeding method of broccoli |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104160946A (en) * | 2014-07-30 | 2014-11-26 | 镇江瑞繁农艺有限公司 | Breeding method for cytoplasmic male sterility line and maintainer line of brassica oleracea |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104160946A (en) * | 2014-07-30 | 2014-11-26 | 镇江瑞繁农艺有限公司 | Breeding method for cytoplasmic male sterility line and maintainer line of brassica oleracea |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113287471A (en) * | 2021-04-22 | 2021-08-24 | 云南省农业科学院热区生态农业研究所 | Stock breeding method of broccoli |
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