CN108184656B - A kind of selection of the dedicated japonica rice variety in fish pond - Google Patents
A kind of selection of the dedicated japonica rice variety in fish pond Download PDFInfo
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- CN108184656B CN108184656B CN201810145661.1A CN201810145661A CN108184656B CN 108184656 B CN108184656 B CN 108184656B CN 201810145661 A CN201810145661 A CN 201810145661A CN 108184656 B CN108184656 B CN 108184656B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of selections of the dedicated japonica rice variety in fish pond, this method is that parent hybridizes with temperature sensitive type japonica rice and tall and big strong rod-type long-grained nonglutinous rice, and it is returned with temperature sensitive type japonica rice, the gene of control plant type is carried by molecular mark technology screening, strong and morning jointing stage of earing the sizing rice varieties through interval sowing screening temperature sensitivity, the kind not only has that tall and big strong bar, plant type is compact, tiller is more, disease-resistant and high yield characteristic, is suitable for fish pond rice special；Also with the characteristic of high zinc content.
Description
Technical field
The present invention relates to a kind of selections of the dedicated japonica rice variety in rice breeding field more particularly to fish pond.
Background technique
With the increasingly raising of living standard, great change has occurred in the eating patterns of China resident；Wherein, the common people are to excellent
The demand of matter aquatic products is in substantial increase, so that culture fishery development is swift and violent, it is even more to produce high benefit in land of plenty
Aquaculture and kind rice produce grain significant contradiction.
At the same time, during the feeding of the special aquatic products animal such as freshwater shrimp and lake crab, since feeding habits lead to feed
Utilization rate it is low, in addition artificially launch fertilizer, the significant exceeded of the nutrients such as Water, phosphorus is caused, so as to cause increasingly
The eutrophication of the farms such as more lake, reservoir, pond and surrounding body.So the improvement of water eutrophication is aquatic products
Cultivate sustainable development and the dirty important topic of agricultural drop, research and development clean-up effect is good, high efficiency novel crops and vertical
Body agricultural be particularly important with urgently.
The breeding of fish pond rice special kind helps to establish using efficient ecological type rice as the purification of the breeding water body of core
New technology, while solving agricultural environment pollution, more preferable promotion economic benefit, and the environmental superior rice that can keep the safety in production, and
The new way for having expanded Rice Production innovates the new farming mode of the comprehensive breeding in land of plenty with growing with each passing hour.
But the research about the selection of fish pond rice special kind does not have been reported that also at present, it is also not relevant
Rice special kind passes through authorization.Since fish pond generally has the higher depth of water, it is higher to it requires that rice special kind will have
Plant height；And in order to ensure the fish, shrimp, crab of cultivation will not because of high temperature and yield reduce, in full summer high temperature arrive before fish pond
It can be cooled down by way of filling deep water；Although and existing individual conventional japonica rices and some Japonica Hybrid maturity period plant heights also phase
To higher, but have the characteristics that photosensitivity is strong, when being in short-day weather, can not ear jointing, and summer High Temperature is caused to arrive
Plant height before is lower, generally at 40~50 centimetres or so, can not adapt to fish pond and fill deep water mode resistant to high temperatures, easily cause yield
The problems such as low, so being not suitable as fish pond rice special.
Therefore, it is necessary to provide a kind of selection of dedicated japonica rice variety in fish pond to overcome above-mentioned technical problem.
Summary of the invention
The sizing strain that the selection is bred as has temperature sensitive properties, can show tall and big strong bar and plant type is tight
The economical character to gather is suitable for the japonica rice variety as the comprehensive new farming mode of breeding of fishing/rice.
Specific technical solution is as follows:
A kind of selection of the dedicated japonica rice variety in fish pond, comprising:
(1) select temperature sensitive type japonica rice for parent A, tall and big strong rod-type long-grained nonglutinous rice is parent B, using parent A as male parent, by parent A
Hybridize with parent B, obtains F1For seed；
(2) F is planted1In generation, is returned with parent A, obtains backcrossing BC1F1For seed；
(3) BC is planted1F1Generation, selfing harvest BC1F2For seed；
(4) BC is planted1F2In generation, extracts the DNA of plant, and using molecular mark technology, first selection carries tiller angle
The single plant of controlling gene is spent, then by identifying that indica rice type therefrom selects the single plant of japonica-cline type；Then, it is further chosen to
Single plant with tall and big strong bar feature when the ripe phase harvests BC1F3For seed；
(5) BC is planted1F3In generation, is selected to have temperature sensitive properties and be eared morning jointing stage by way of interval sowing
Strain harvests BC1F4For seed；
(6) BC is planted1F4In generation, selects before Basalinternodelength, heading jointing 110 centimetres of plant height >, and the strain at the maturity period
The strain of 160 centimetres of high > harvests BC1F5For seed；
(7) BC is planted1F5Generation and BC1F6In generation, is continuously marked with rice blast resistance composite index < 9 grade and rice quality up to country
The standard selected strain of quasi- three-level or more harvests BC1F7For seed；
(8) BC is planted1F7In generation, selects tall and big strong bar, the strain that plant type is compact and disease-resistant, rice quality is excellent, it is special to obtain fish pond
Use japonica rice variety.
160 centimetres of plant height > and 1.0 centimetres of Trunk diameter > when heretofore described tall and big strong bar refers to the maturity period
Plant.The temperature sensitive type refers to responsive to temperature type, i.e., sensitive to the accumulated temperature of growing environment, and early take out can be realized by planting early enough
Fringe is mature；The plant type is compact to refer to rice tillering angle small (< 25 degree), stem vertical growth.The Basalinternodelength
Refer to that rice can reach 8-10 centimetres close to the extended length of each and every one several internodes on ground.
It is respectively selfing for the mode of reproduction of plant in step (4)~(8).
Existing routine or Japonica Hybrid it is also higher although maturity period plant height, because light sensitive property it is strong, early period does not arrive short
Plant height always very short (40-50 centimetres), is not suitable for planting in fish pond when sunshine weather.
Preferably, the parent A is ZDJR01.
ZDJR01 is Zhejiang University's breeding responsive to temperature type japonica rice strain, can give in or be purchased from agricultural college, Zhejiang University.
The kind plant is short and small (90 centimetres of plant height), and stem is thin (0.5 centimetre of diameter), and plant type is compact, with general common japonica rice to photosensitive
Feel significantly different, show strong temperature sensitivity, being can the japonica rice that early ears of planting early enough.
Preferably, the parent B is Zhejiang rice No. 1.
Zhejiang rice No. 1 be Zhejiang University's breeding and authorize New species right in plant rice varieties (new varieties Authorization Notice No.:
CNA20120031.5), agricultural college, Zhejiang University can be purchased from.The kind plant type is tall and big (185 centimetres of plant height), the sturdy (diameter of stem
1.5 centimetres), plant type is loose, the typical long-grained nonglutinous rice insensitive to light.
It selects ZDJR01 and Zhejiang rice No. 1 to be hybridized, then is returned with ZDJR01, the compact temperature of the tall and big strong bar of breeding, plant type
Quick type japonica rice strain.
Further, in step (4), the tillering angle control gene is TAC1 gene；Specific molecular labeling auxiliary
The method (design of primers and operating method) of breeding referring to Yu B, Lin Z, Li H, Li X, Li J, Wang Y, Zhang X,
Zhu Z,Zhai W,Wang X,Xie D,Sun C(2007).TAC1,a major quanti tative trait locus
controlling tiller angle in rice.Plant J,52,891-898。
Further, in step (4), for identifying the molecular labeling of indica rice type for InDel label.InDel label
Method utilizes Indica or japonica [J] Fujian agriculture of the derivative strain of InDel Marker Identification japonica Cross referring to: such as Chen Pingping
Report, 2014,29 (6): 519-523.
Preferably, passing through the method for interval sowing selection temperature sensitive type strain are as follows: mid-April to May in step (5)
The middle ten days carries out interval sowing, is divided into for 7 phases, is spaced 7 days for each issue；Heading period is sowed than the 7th phase in the single plant for selecting for the 1st phase sowed
Single plant heading period long 20 days or more single plants.
Further, in step (7), resisted with the rice blast that standard GB/T/T 15790-2009 is foundation identification plant
Property composite index；Eating rice varieties quality with Ministry of Agriculture professional standard NY/T593-2002 is the rice product according to identification plant
Matter.
Compared with prior art, the invention has the benefit that
(1) the method for the present invention with temperature sensitive type japonica rice and tall and big strong rod-type long-grained nonglutinous rice is that parent hybridizes, and with temperature sensitive type round-grained rice
Rice backcrossing, the gene of control plant type is carried by molecular mark technology screening, quick through interval sowing screening temperature
Perceptual strong and morning jointing stage of earing sizing rice varieties, the kind not only have that tall and big strong bar, plant type is compact, tiller is more, anti-
The characteristic of disease and high yield, is suitable for fish pond rice special；Also with the characteristic of high zinc content.
(2) the dedicated japonica rice variety in fish pond that the method for the present invention is bred as, not only facilitating foundation with efficient ecological type rice is
The breeding water body technology for clarifying system of core, solves the problems, such as N/P emission reduction, and can produce environmental superior rice, expands innovation not
It is same as " fish pond kind rice " new farming mode of rice field fish culture.
Specific embodiment
Below with reference to embodiment, present invention is further described in detail.
Embodiment 1
1, breeding material:
(1) with ZDJR01 for parent A.
ZDJR01 is the responsive to temperature type japonica rice strain of Zhejiang University's breeding, can give in or be purchased from Zhejiang University's agronomy
Institute.The kind plant is short and small (basal internode is short, only 36 centimetres of plant height before jointing of earing, 90 centimetres of maturity period plant height), stem
Relatively thin, plant type is compact, significantly different with general photosensitive common japonica rice, shows strong temperature sensitivity, is that can pass through
The early japonica rice variety for realizing that early heading is mature is broadcast in advance.
(2) with Zhejiang rice No. 1 for parent B.
Zhejiang rice No. 1 be Zhejiang University's breeding and authorize New species right in plant rice varieties (new varieties Authorization Notice No.:
CNA20120031.5), agricultural college, Zhejiang University can be purchased from.The kind plant type is tall and big (Basalinternodelength can reach 8-10 centimetres),
123 centimetres of plant height before heading jointing, 185 centimetres of maturity period plant height), stem is sturdy, and plant type is loose, the typical case insensitive to light
Long-grained nonglutinous rice.
2, the Breeding Process of the dedicated japonica rice variety in fish pond are as follows:
(1) 2012 year summer and autumn, Zhejiang Hangzhou select responsive to temperature type japonica rice variety ZDJR01 for parent A, tall and big strong bar
Rice Zhejiang rice No. 1 is parent B, and parent A hybridizes as male parent with parent B, obtains F1Generation (Zhejiang ZDJR01/ rice No. 1) 5, seed；
F is planted in (2) 2013 year spring, Hainan Lingshui1Generation 5 plants of (Zhejiang ZDJR01/ rice No. 1), as male parent, with ZDJR01
Backcrossing obtains backcrossing BC1F1Generation (Zhejiang ZDJR01//ZDJR01/ rice No. 1) 12, seed；
(3) 2013 year summer, Zhejiang Hangzhou, plantation backcrossing BC1F1For 11 plants of plant, harvest backcrossing BC1F2For seed 2743
Grain.
(4) 2014 year spring, Hainan Lingshui, by single-strain planting BC1F2In generation, extracts BC1F2For the DNA of plant, using molecule
Supplementary means is marked, selection carries single plant 1109 of tillering angle controlling gene TAC1, recycles molecular labeling nondominant hand
Section identifies indica rice type, selects single plant 282 of japonica-cline type, and single plant 58 of tall and big strong bar are selected when mature, harvests BC1F3
For seed；
(5) 2014 year summer, Zhejiang Hangzhou plant BC by strain1F3Generation, since April 15 to mid-May every 7 days
Divide 7 phases sowing (I, II, III, IV, V, VI, VII), first stage sowing is selected to sow early heading 20 days or more temperature than VII phase
The excellent japonica rice strain of responsive type 37 harvests BC1F4For seed；
(6) 2015 year summer, Zhejiang Hangzhou plant BC1F4In generation, was sowed before mid-May, selected basal internode very long, took out
Be greater than strain 29 of 110 centimetres before fringe jointing, then therefrom selection maturity period plant height up to 160 centimetres or more of excellent strain 18
It is a, harvest BC1F5For seed；
BC is planted in (7) 2016 year spring, Hainan Lingshui1F5In generation, selects the excellent product of rice blast resistance composite index < 9 grade
It is 11, harvests BC1F6For seed；
(8) 2016 year summer, Zhejiang Hangzhou plant BC1F6In generation, selects quality to promulgate up to the Ministry of Agriculture by quality breeding
Third or more excellent strain 3 of edible high quality paddy, harvest BC1F7Generation；
(9) 2017 year summer, Zhejiang Hangzhou plant BC by strain1F7Generation, the temperature sensitive type for selecting tall and big strong bar, plant type compact
Japonica rice, and disease-resistant and quality sizing strain 1 is taken into account to get the dedicated japonica rice variety in fish pond.
The dedicated japonica rice variety in the fish pond of the present embodiment breeding is temperature sensitive type japonica rice, and showing tall and big strong bar, (basal internode is very
Long, 113 centimetres of plant height before jointing of earing, 164 centimetres of maturity period plant height), Trunk diameter slightly up to 1.3 centimetres, plant type is compact, tiller
Angle be 15 degree or sos, show big fringe grain it is more (spike length be averaged 23.6 centimetres, every fringe be averaged 347), 25 grams of mass of 1000 kernel, crop field kind
Per mu yield 700kg is planted, is planting about 350 kgs/acre of yield by 60 cm x, 60 centimetres of fish ponds.
After screening obtains above-mentioned sizing rice, the sizing rice and its parent are specially planted in the crab pool of lake, carried out
Breeding combines plantation, and is surveyed to the mineral nutrient elements such as calcium, iron, zinc in its seed and several pesticide residue contents
Fixed, concrete outcome is shown in Table 1.
The main mineral nutrition and content of beary metal of 1 Zhejiang rice of table No. 1, ZDJR01 and approved product compare
Remarks: the measuring method of each index is as follows in table 1: calcium: GB/T5009.92-2003；Iron: GB/
T5009.90-2003；Zinc: GB/T5009.14-2003；Carbofuran: NY/T 761-2008 butachlor: GB/T5009.164-
2003；Poison cicada with poison: GB/T5009.145-2003；Isoprothiolane: GB/T5009.155-2003.
On beneficial Mineral nutrients, iron content and 2 parents are similar in approved product, but may be because cultivating in the pool
The reason of lake crab high there are crab shell calcium constituent, the calcium content in approved product are significantly higher than 2 parents.Meanwhile always by fish pond
" adverse circumstance " stress of deep water cultivation, the content of the zinc in approved product are higher than 2 parents.
Carbofuran, butachlor are not detected in main pesticide residue aspect, 2 parents and approved product and poison cicada agriculture with poison
Medicine residual；And because occurring more serious rice blast disease in local paddy field, serious disease also occurs for 2 parents, inspection
Minimal amount of agricultural chemical ' Daowenling ' residual is measured, but is also to meet the regulation of national safety standard, and approved product does not detect rice
Pest spirit.
Therefore, this sizing rice is not only more safer than parent production when carrying out feeding kind of combination, is more favorable to promote
Into human intelligence development, skeleton development, growth and development and nutrition and health care element, especially calcium and zinc natural enhancement, it is nontoxic and
It is without side-effects.
Technical solution of the present invention and beneficial effect is described in detail in embodiment described above, it should be understood that
Above is only a specific embodiment of the present invention, it is not intended to restrict the invention, it is all to be done in spirit of the invention
Any modification, supplementary, and equivalent replacement etc., should all be included in the protection scope of the present invention.
Claims (2)
1. a kind of selection of the dedicated japonica rice variety in fish pond characterized by comprising
(1) select temperature sensitive type japonica rice for parent A, tall and big strong rod-type long-grained nonglutinous rice is parent B, using parent A as male parent, by parent A and parent
This B is hybridized, and F is obtained1For seed；
(2) F is planted1In generation, is returned with parent A, obtains backcrossing BC1F1For seed；
(3) BC is planted1F1Generation, selfing harvest BC1F2For seed；
(4) BC is planted1F2In generation, extracts the DNA of plant, and using InDel molecular mark technology, first selection carries TAC1
The single plant of controlling gene, then by identifying that indica rice type therefrom selects the single plant of japonica-cline type；Then, further selection is mature
Single plant with tall and big strong bar feature when the phase harvests BC1F3For seed；
(5) BC is planted1F3In generation, carries out interval sowing mid-April to mid-May, was divided into for 7 phases by way of interval sowing,
Each issue be spaced 7 days；Heading period was sowed than the 7th phase in the single plant for selecting for the 1st phase sowed single plant heading period long 20 days or more
Single plant, select have temperature sensitive properties and ear jointing stage morning strain, harvest BC1F4For seed；
(6) BC is planted1F4In generation, selects before Basalinternodelength, heading jointing 110 centimetres of plant height >, and the plant height > at the maturity period
160 centimetres of strain harvests BC1F5For seed；
(7) BC is planted1F5Generation and BC1F6In generation, continuously reaches national standard three with rice blast resistance composite index < 9 grade and rice quality
Grade or more standard selected strain, harvest BC1F7For seed；
(8) BC is planted1F7In generation, selects tall and big strong bar, the strain that plant type is compact and disease-resistant, rice quality is excellent, obtains the dedicated round-grained rice in fish pond
Rice varieties；
The parent A is ZDJR01；The parent B is Zhejiang rice No. 1.
2. selection according to claim 1, which is characterized in that in step (7), with standard GB/T/T 15790-
2009 be the rice blast resistance composite index according to identification plant；Rice varieties are eaten with Ministry of Agriculture professional standard NY/T593-2002
Quality is the rice quality according to identification plant.
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| CN106900539A (en) * | 2017-02-14 | 2017-06-30 | 浙江大学 | A kind of high-resistance starch and the method for breeding nonglutinous rice of zinc high polymerization |
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