CN101647387A - Distant hybridization breeding method among three categories of shoal spartina alterniflora loisel, maize and rice - Google Patents
Distant hybridization breeding method among three categories of shoal spartina alterniflora loisel, maize and rice Download PDFInfo
- Publication number
- CN101647387A CN101647387A CN200910183938A CN200910183938A CN101647387A CN 101647387 A CN101647387 A CN 101647387A CN 200910183938 A CN200910183938 A CN 200910183938A CN 200910183938 A CN200910183938 A CN 200910183938A CN 101647387 A CN101647387 A CN 101647387A
- Authority
- CN
- China
- Prior art keywords
- corn
- paddy rice
- rice
- spartina alterniflora
- alterniflora loisel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a distant hybridization breeding method among three categories of shoal spartina alterniflora loisel, maize and rice. The method comprises the following steps: carrying out hybridization by using maize as a male parent and using rice as a female parent to obtain a first hybridized generation of the maize and the rice; carrying out hybridization by using shoal spartina alterniflora loisel as a male parent and using the first hybridized generation of the maize and the rice as a female parent to obtain a distant hybridized seed among the three categories of the shoal spartina alterniflora loisel, the maize and the rice; and carrying out pedigree selective breeding on the distant hybridized seed among the three categories of the shoal spartina alterniflora loisel, the maize and the rice so as to select and breed out a salt resistant and high-yield seed. The invention selects and breeds a new variety suitable for growing on shoals by excavating and creating the high-quality high-yield economic property of the maize and the rice and germplasm resources of salt resistant genes of the spartina alterniflora loisel, and the new variety can be used as a grain and a feed. The invention has huge economic value and social meaning to develop and utilize wide shoals of China.
Description
Technical field:
The present invention relates to a kind of plant hybridization breeding method.
Background technology:
At present, identified 14 kinds of rice grass in the world, that cultivated area is the widest on China seabeach is the spartina alterniflora loisel.Spartina alterniflora loisel (Spartina alterniflora) introduced China by professor Zhong Chongxin of Nanjing University from the U.S. in 1979.Belong to the grass family herbaceos perennial, have very strong salt tolerant, the anti-ability of flooding, the suitableeest intertidal zone, seabeach of being born in.Have the beach of guarantor shore protection, created land with silt, effects such as seabeach ecotope are improved at the greening seabeach.Because the spartina alterniflora loisel plant is tall and big, the grass yield height is fit to plant-eating animals such as free range husbandry ox, sheep, can be used as animal and fowl fodder, fish food, fertilizer and fuel, and widely people pay attention to.At present, the jiangsu coast beach major part spartina alterniflora loisel that growing.
This patent invention and applicant Chen Qikang researcher are engaged in solonchak agricultural, tidal land research and development 35 years, and research and utilization rice grass were raised cattle and sheep 35 years as feed, resolved motherland's tidal land is built up green Great Wall.Introduce a fine variety the rice grass in 1975, introduced a fine variety the spartina alterniflora loisel in 1980, set up 80,000 mu in tidal land rice grass pasture, created first rice grass Yang Chang---Qidong City Huanghai Sea sheep known for its fine thick wool field, Jiangsu Province of China.Bear State Scientific and Technological Commission's " experiment of pasture, beach " and Jiangsu Province, 8 scientific research projects of the Municipal Commission of Science and Technology.Utilize the careless free range husbandry cattle and sheep of rice, year raising cattle and sheep 3000 bulls, " experiment of pasture, beach " project was accredited as domestic initiation in 1985.
Discover, good characteristic such as a rice grass has salt tolerant really, anti-ly floods, fertility is strong, but rice grass seeds seed is little, and ripening rate is low, and is difficult to results.
Should study and utilize rice grass a salt tolerant, anti-ly flood, efficient genetic resources such as fertility is strong.
Should study and utilize full seed, the good quality and high output high efficiency gene resource of paddy rice.
Paddy rice belongs to grass family Oryza common cultivation kind.Paddy rice is one of main cereal crops, and it is staple food with rice that the whole world has 1/2 population approximately, and China's paddy rice cultivated area and gross yield all account for the No. 1 in the world.Variety resources of rice is rich and varied, and seed is full, and output height, straw can be used as the cattle and sheep feed again, for the improvement crop varieties provides good germ plasm resource condition.
Should study and utilize the stem of corn sturdy, efficient genetic resources such as drought-enduring, full seed.
Zea grass family Zea.Corn is one of the cereal crops the most widely that distribute in the world, and cultivated area is only second to wheat and paddy rice.The corn maturation is all arranged in the world every month in the whole year.Corn is the most important cereal crops of the U.S., and output accounts for half of world wide production, and wherein about 2/5 for export trade.China produces corn per year and accounts for the second place of the world, secondly is Brazil, Mexico, Argentina.Corn is in a large number as food, feed, the raw material of industry.As main food, is one of main food of northern China and southwestern mountain area and other winterbourne area people in many areas; Corn is the main body of cereals feed, also is the main energy feed of China; Corn is the primary raw material of industrial alcohol and liquor.
To one of optimum scientific method of rice grass species Ecological Control is substitution effect, can substitute the rice grass with rice grass and hybrid rice seed, and this is the difficult problem that this patent invention and applicant Chen Qikang researcher are researching and solving always.Distant hybridization can import the beneficial gene of different genera plant, improves and improve the economic characters of plant variety, incomparable diversity and the newtype of nearly edge hybridization occur.
Summary of the invention:
The object of the present invention is to provide a kind of can selecting to be fit to tidal land spartina alterniflora loisel, corn, the tidal land spartina alterniflora loisel of paddy rice ternary inter-genera distant hybridization kind, corn, the paddy rice ternary inter-genera distant hybridization breeding method that tidal land is grown.
Technical solution of the present invention is:
A kind of tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization breeding method is characterized in that: comprise the following steps:
(1) is that male parent, paddy rice are that female parent is hybridized with the corn, gets the first-filial generation of corn and paddy rice;
(2) be that the first-filial generation of male parent, corn and paddy rice is that female parent is hybridized with the tidal land spartina alterniflora loisel, tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization kind;
(3) tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization kind are carried out the pedigree seed selection, select salt tolerant, high-yielding seed.
Paddy rice emasculating method hot water emasculation method when hybridizing in the step (1): at this full heading time of father and mother, every morning is filled with warm water with basin before paddy rice blooms, water temperature is transferred to 41-42 ℃, spike of rice is immersed in the warm water handled 10 minutes, water temperature is controlled at 41-42 ℃, to reach gametocidal effect, after warm water is handled, open grain husk successively, the flowers are in blossom excision is not opened when intact grain husk flower, having opened the flower pesticide of grain husk also will remove, when removing flower pesticide, cut off 1/3 of clever shell top, to improve stigma exposing ratio and ripening rate; Castration is cut behind the grain husk immediately bagging and is isolated, the numbering of listing, and the clip corn colored fringe that has a male powder carries out artificial pollination then, and hybridisation events is afterwards write down in pollination.
When in step (2) when hybridization, castrate the first-filial generation of corn and paddy rice, at this full heading time of father and mother, every morning is before corn ♂ * paddy rice ♀ first-filial generation is bloomed, fill with warm water with basin, water temperature is transferred to 41-42 ℃, meeting growth, castrate in the corn ♂ * paddy rice ♀ first-filial generation fringe immersion warm water that requires and handled 10 minutes, water temperature is controlled at 41-42 ℃, to reach gametocidal effect, after warm water is handled, open grain husk successively, the grain husk flower that the flowers are in blossom must excise out when intact has been opened the flower pesticide of grain husk and also will have been removed, when removing flower pesticide, cut off 1/3 of clever shell top, to improve stigma exposing ratio and ripening rate; Castration is cut behind the grain husk immediately bagging and is isolated, the numbering of listing, and the clip spartina alterniflora loisel colored fringe that has a male powder carries out artificial pollination then, and hybridisation events is afterwards write down in pollination.
The concrete grammar of pedigree seed selection comprises the following steps: successively
(1) tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization kind are planted on greater than 5 ‰ tidal land in salt content, select the seedling of normal growth;
(2) with the seedling of normal growth after growth and maturity, select the individual plant of thousand grammes per square metres greater than 15 grams, single receipts singles, the results seed, and plant into plant in salt content on greater than 5 ‰ tidal land next year, therefrom select the individual plants of thousand grammes per square metres again greater than 15 grams, the results seed, and plant into strain system on greater than 5 ‰ tidal land in salt content in next year;
(3) select strain system and the individual plants of thousand grammes per square metres of individual plant greater than 20 grams from above-mentioned strain is, the upgrading of strain system enters the strain evaluation next year;
(4) select the strains system of thousand grammes per square metres of individual plant greater than 20 grams from the strain system that enters the strain evaluation, tentatively breeding seed carries out production test, breeding seed then.
The present invention excavates and innovation by corn, paddy rice good quality and high output economic characters and spartina alterniflora loisel's resistant gene of salt germ plasm resource, selects the new varieties that are fit to the tidal land growth, has good quality and high output salt tolerant, performance, can raise dual-purpose for grain.The wide tidal land of development and utilization China had huge economic and social effect.
Tidal land spartina alterniflora loisel ♂ * (corn ♂ * paddy rice ♀) ♀ ternary distant hybridization test effect:
Wherein: Su Yu F
133 are meant that the present application people trained short 64S/04141 (♀) hybridization in 2005 with corn variety Suyunuo No.1 (♂) and paddy rice and obtains the plant seed No. 33.The F of cultivation in 2006
1Plant, spartina alterniflora loisel (♂) * (Suyunuo No.1 (♂) * paddy rice is trained short 64S/04141 (♀)) carried out three way cross, to observe the three way cross effect.
The invention will be further described below in conjunction with embodiment.
Embodiment:
A kind of tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization breeding method, it comprises the following steps:
(1) is that male parent, paddy rice are that female parent is hybridized with the corn, gets the first-filial generation of corn and paddy rice, the first-filial generation that obtains is carried out Molecular Detection and cytological analysis, confirm that it contains the genetic constitution of corn and paddy rice;
(2) be that the first-filial generation of male parent, corn and paddy rice is that female parent is hybridized with the tidal land spartina alterniflora loisel, get tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization kind, the crossbreed that obtains is carried out Molecular Detection and cytological analysis, confirm that it contains corn, paddy rice and spartina alterniflora loisel's genetic constitution;
(3) tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization kind are carried out the pedigree seed selection, select salt tolerant, high-yielding seed.
Paddy rice emasculating method hot water emasculation method when hybridizing in the step (1): at this full heading time of father and mother, every morning is filled with warm water with basin before paddy rice blooms, water temperature is transferred to 41-42 ℃, spike of rice is immersed in the warm water handled 10 minutes, water temperature is controlled at 41-42 ℃, to reach gametocidal effect, after warm water is handled, open grain husk successively, the flowers are in blossom excision is not opened when intact grain husk flower, having opened the flower pesticide of grain husk also will remove, when removing flower pesticide, cut off 1/3 of clever shell top, to improve stigma exposing ratio and ripening rate; Castration is cut behind the grain husk immediately bagging and is isolated, the numbering of listing, and the clip corn colored fringe that has a male powder carries out artificial pollination then, and hybridisation events is afterwards write down in pollination.
When in step (2) when hybridization, castrate the first-filial generation of corn and paddy rice, at this full heading time of father and mother, every morning is before corn ♂ * paddy rice ♀ first-filial generation is bloomed, fill with warm water with basin, water temperature is transferred to 41-42 ℃, meeting growth, castrate in the corn ♂ * paddy rice ♀ first-filial generation fringe immersion warm water that requires and handled 10 minutes, water temperature is controlled at 41-42 ℃, to reach gametocidal effect, after warm water is handled, open grain husk successively, the grain husk flower that the flowers are in blossom must excise out when intact has been opened the flower pesticide of grain husk and also will have been removed, when removing flower pesticide, cut off 1/3 of clever shell top, to improve stigma exposing ratio and ripening rate; Castration is cut behind the grain husk immediately bagging and is isolated, the numbering of listing, and the clip spartina alterniflora loisel colored fringe that has a male powder carries out artificial pollination then, and hybridisation events is afterwards write down in pollination.
The concrete grammar of pedigree seed selection comprises the following steps: successively
(1) tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization kind are planted on greater than 5 ‰ tidal land in salt content, select the seedling of normal growth;
(2) with the seedling of normal growth after growth and maturity, select the individual plant of thousand grammes per square metres greater than 15 grams, single receipts singles, the results seed, and plant into plant in salt content on greater than 5 ‰ tidal land next year, therefrom select the individual plants of thousand grammes per square metres again greater than 15 grams, the results seed, and plant into strain system on greater than 5 ‰ tidal land in salt content in next year;
(3) select strain system and the individual plants of thousand grammes per square metres of individual plant greater than 20 grams from above-mentioned strain is, carry out molecular marker assisted selection, confirm that it contains corn, paddy rice and spartina alterniflora loisel's genetic constitution, the upgrading of strain system enters the strain evaluation next year;
(4) select the strains system of thousand grammes per square metres of individual plant greater than 20 grams from the strain system that enters the strain evaluation, tentatively breeding seed carries out production test, breeding seed then.
Claims (4)
1, a kind of tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization breeding method is characterized in that: comprise the following steps:
(1) is that male parent, paddy rice are that female parent is hybridized with the corn, gets the first-filial generation of corn and paddy rice;
(2) be that the first-filial generation of male parent, corn and paddy rice is that female parent is hybridized with the tidal land spartina alterniflora loisel, tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization kind;
(3) tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization kind are carried out the pedigree seed selection, select salt tolerant, high-yielding seed.
2, tidal land spartina alterniflora loisel according to claim 1, corn, paddy rice ternary inter-genera distant hybridization breeding method, it is characterized in that: paddy rice emasculating method hot water emasculation method when hybridizing in the step (1): at this full heading time of father and mother, every morning is before paddy rice blooms, fill with warm water with basin, water temperature is transferred to 41-42 ℃, handling 10 minutes in the spike of rice immersion warm water, water temperature is controlled at 41-42 ℃, to reach gametocidal effect, after warm water is handled, open grain husk successively, the flowers are in blossom excision is not opened when intact grain husk flower has been opened the flower pesticide of grain husk and also will have been removed, when removing flower pesticide, cut off 1/3 of clever shell top, to improve stigma exposing ratio and ripening rate; Castration is cut behind the grain husk immediately bagging and is isolated, the numbering of listing, and the clip corn colored fringe that has a male powder carries out artificial pollination then, and hybridisation events is afterwards write down in pollination.
3, tidal land spartina alterniflora loisel according to claim 1, corn, paddy rice ternary inter-genera distant hybridization breeding method, it is characterized in that: when step (2) when hybridization castrates the first-filial generation of corn and paddy rice, at this full heading time of father and mother, every morning is before corn ♂ * paddy rice ♀ first-filial generation is bloomed, fill with warm water with basin, water temperature is transferred to 41-42 ℃, meeting growth, castrate in the corn ♂ * paddy rice ♀ first-filial generation fringe immersion warm water that requires and handled 10 minutes, water temperature is controlled at 41-42 ℃, to reach gametocidal effect, after warm water is handled, open grain husk successively, the grain husk flower that the flowers are in blossom must excise out when intact, having opened the flower pesticide of grain husk also will remove, when removing flower pesticide, cut off 1/3 of clever shell top, to improve stigma exposing ratio and ripening rate; Castration is cut behind the grain husk immediately bagging and is isolated, the numbering of listing, and the clip spartina alterniflora loisel colored fringe that has a male powder carries out artificial pollination then, and hybridisation events is afterwards write down in pollination.
4, according to claim 1,2 or 3 described tidal land spartina alterniflora loisels, corn, paddy rice ternary inter-genera distant hybridization breeding method, it is characterized in that: the concrete grammar of pedigree seed selection comprises the following steps: successively
(1) tidal land spartina alterniflora loisel, corn, paddy rice ternary inter-genera distant hybridization kind are planted on greater than 5 ‰ tidal land in salt content, select the seedling of normal growth;
(2) with the seedling of normal growth after growth and maturity, select the individual plant of thousand grammes per square metres greater than 15 grams, single receipts singles, the results seed, and plant into plant in salt content on greater than 5 ‰ tidal land next year, therefrom select the individual plants of thousand grammes per square metres again greater than 15 grams, the results seed, and plant into strain system on greater than 5 ‰ tidal land in salt content in next year;
(3) select strain system and the individual plants of thousand grammes per square metres of individual plant greater than 20 grams from above-mentioned strain is, the upgrading of strain system enters the strain evaluation next year;
(4) select the strains system of thousand grammes per square metres of individual plant greater than 20 grams from the strain system that enters the strain evaluation, tentatively breeding seed carries out production test, breeding seed then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910183938A CN101647387A (en) | 2009-08-13 | 2009-08-13 | Distant hybridization breeding method among three categories of shoal spartina alterniflora loisel, maize and rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910183938A CN101647387A (en) | 2009-08-13 | 2009-08-13 | Distant hybridization breeding method among three categories of shoal spartina alterniflora loisel, maize and rice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101647387A true CN101647387A (en) | 2010-02-17 |
Family
ID=41669800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910183938A Pending CN101647387A (en) | 2009-08-13 | 2009-08-13 | Distant hybridization breeding method among three categories of shoal spartina alterniflora loisel, maize and rice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101647387A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102783408A (en) * | 2012-08-20 | 2012-11-21 | 江苏丰源种业有限公司 | Method for killing male of japonica rice by warm water |
| CN105145341A (en) * | 2015-09-10 | 2015-12-16 | 陈启康 | Positive and reversed distant hybridization method of tideland smooth cordgrass and rice |
| CN107155872A (en) * | 2017-07-03 | 2017-09-15 | 河北省农林科学院旱作农业研究所 | Improve the warm water immersion column cap method of wheat ×maize hybridization pollination success rate |
| CN108040864A (en) * | 2017-12-28 | 2018-05-18 | 云南省农业科学院粮食作物研究所 | A kind of rice × corn hybridization generates the haploid method of rice |
| CN108476975A (en) * | 2018-04-13 | 2018-09-04 | 吉林省农业科学院 | One cultivates peanut emasculating method |
-
2009
- 2009-08-13 CN CN200910183938A patent/CN101647387A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102783408A (en) * | 2012-08-20 | 2012-11-21 | 江苏丰源种业有限公司 | Method for killing male of japonica rice by warm water |
| CN102783408B (en) * | 2012-08-20 | 2015-04-01 | 江苏丰源种业有限公司 | Method for killing male of japonica rice by warm water |
| CN105145341A (en) * | 2015-09-10 | 2015-12-16 | 陈启康 | Positive and reversed distant hybridization method of tideland smooth cordgrass and rice |
| CN107155872A (en) * | 2017-07-03 | 2017-09-15 | 河北省农林科学院旱作农业研究所 | Improve the warm water immersion column cap method of wheat ×maize hybridization pollination success rate |
| CN108040864A (en) * | 2017-12-28 | 2018-05-18 | 云南省农业科学院粮食作物研究所 | A kind of rice × corn hybridization generates the haploid method of rice |
| CN108476975A (en) * | 2018-04-13 | 2018-09-04 | 吉林省农业科学院 | One cultivates peanut emasculating method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ashok Kumar et al. | Recent advances in sorghum genetic enhancement research at ICRISAT | |
| CN101336611B (en) | Breeding method of good quality, double-low, disease-resistant, high yield, lodging-resistant rape | |
| CN106034683A (en) | Forage grass planting method | |
| CN106342679A (en) | Breeding method of perennial rice and application | |
| CN104429934A (en) | Method for breeding drought-resistant hybrid rice | |
| CN101647387A (en) | Distant hybridization breeding method among three categories of shoal spartina alterniflora loisel, maize and rice | |
| CN109618911A (en) | A kind of winter wheat strange land of simple and effective low cost adds for breeding method | |
| CN107046993A (en) | A kind of breeding method of sorghum | |
| CN101919353A (en) | Breeding method of hybrid rice seed | |
| CN101647388A (en) | Method for breeding tidal marsh spartina, sweet sorghum, rice hybrid feed and energy resource crop | |
| CN103355080B (en) | A kind of cultural method of perennial forage maize | |
| CN100397977C (en) | Breed method for rice reproduction heterosis fixation | |
| CN107155866A (en) | The method that perennial forage maize is cultivated using non-multiple corn allopolyploid | |
| CN102138517A (en) | Breeding method for maize variety | |
| CN110100722B (en) | Breeding method of purple-leaf white-flower cabbage type landscape rape conventional variety | |
| CN102124944A (en) | Breeding method of hybrid corn | |
| CN103461098A (en) | Breeding method for rape | |
| CN102668975A (en) | Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker | |
| CN101006767B (en) | Seashore spartina anglica and rice interspecies distant hybrid breeding method | |
| CN101773065B (en) | Culture and application of No.3 North China hologynic cucumber of hologynic North China type cucumbers | |
| Karlsson et al. | Early growth and development of Ensete ventricosum (Musaceae) seedlings | |
| CN104782371B (en) | A kind of ratooning rice for being suitable for southern ricefish system, which stores, educates cultural method | |
| Saxena et al. | Challenges and opportunities of breeding early maturing pigeonpea hybrids | |
| CN102960238A (en) | Method for breeding new variety of sweet-straw rice for both grain and feed | |
| CN108184656B (en) | A kind of selection of the dedicated japonica rice variety in fish pond |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Chen Qikang Inventor after: Dai Hui Inventor after: Jiang Yinrong Inventor before: Chen Qikang Inventor before: Dai Hui |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: CHEN QIKANG DAI HUI TO: CHEN QIKANG DAI HUI JIANG YINRONG |
|
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20100217 |