CN104429934A - Method for breeding drought-resistant hybrid rice - Google Patents
Method for breeding drought-resistant hybrid rice Download PDFInfo
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- CN104429934A CN104429934A CN201410766615.5A CN201410766615A CN104429934A CN 104429934 A CN104429934 A CN 104429934A CN 201410766615 A CN201410766615 A CN 201410766615A CN 104429934 A CN104429934 A CN 104429934A
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Abstract
The invention discloses a method for breeding drought-resistant hybrid rice. The method comprises the following steps: introducing total DNA of barnyard grass into a restorer R and a maintainer line B, planting the introduced DNA progeny material, harvesting seeds of a single plant to obtain seed rice D1, planting each single plant seed of the D1 in a single plant line area by 10-15 lines, respectively performing mixed harvesting on the single plant to obtain seed rice D2, planting each single plant seed of the D2 by 20 lines, performing single plant harvesting on variant strains of leaf type, ear type and plant type character donor barnyard grass so as to obtain seed rice D3, performing conventional planting on single plant D3, screening to obtain a single plant with good characters so as to obtain seed rice D4, carrying out backcrossing on the selected maintainer line and 650A as well as the D4, thereby obtaining the offspring D5 with C4 plant resources. The method disclosed by the invention is simple and rapid, and the bred rice variety is excellent in drought resistance and has a high economic value.
Description
Technical field
The invention belongs to the technical field of agricultural breeding, be specifically related to the selection of a kind of drought resisting hybrid rice.
Background technology
Global water resources shortage, subterranean water level continuous decrease causes global Droughts frequently to occur.And China is large agricultural country, the agricultural production of arid serious harm China and grain security, the agricultural development of restriction China.Issuing statistical data 2009,2010,2011 according to State Flood Control and Drought Relief Headquarters loses serious in continuous 3 years by drought impact.Arid causes a large amount of losses of national income, seriously constrains the development of China's economic society.Therefore, find or accelerate to cultivate drought-resistant crops new varieties, contribute to stable and promote China's grain-production.
Paddy rice is as one of the staple food crop of China, and sowing area is very large, and paddy rice belongs to semi-aquatic plant, and drought resistance is between xerophytism plant and aquatic property plant.Along with the scarcity day by day of global water resources, arid becomes one of most regional the most serious natural calamity faced.And this disaster produces physiological stress to the growth of semi-aquatic plant, have a strong impact on the normal growth of paddy rice, constrained the sustainable development of Rice Production, brought crisis to grain security.
In order to resist this natural calamity, except adjustment cropping system, strengthen except irrigation ability, employing water-saving culture measure etc., screening or seed selection Drought-Resistant Rice Variety are one of directions of resisting arid Developing Water-saving Agriculture, but in fact the overwhelming majority is conventional upland rice variety in actual applications at present, because Drought-resistant Breeding difficulty is large, science research input far away from rice breeding, in the urgent need to using rice breeding new results fast, to improve upland rice breeding level, and accelerate its application of large.
Summary of the invention
The present invention is directed to the technology shortage of existing environment and drought resisting rice breeding aspect, provide the selection of a kind of drought resisting hybrid rice.
The present invention realizes especially by following technical scheme:
A selection for drought resisting hybrid rice, comprises the following steps:
1) barnyard grass STb gene is imported in restorer R, maintainer B;
2) plant importing DNA progeny material, individual plant sowing obtains D1 seed rice;
3) each individual plant kind 10 ~ 15 row of individual plant capable district plantation D1, and individual plant is mixed respectively gather in the crops to obtain D2 seed rice;
4) 20 row are planted to each individual plant kind of D2, individual plant sowing is carried out to the variant of the inclined donor barnyard grass of blade profile, fringe type and characters with plant and obtains D3 seed rice;
5) general planting D3 individual plant kind, the individual plant that screening acquired character is excellent obtains D4 seed rice;
6) choose maintainer and 650A backcrosses to D4, obtain there is C4 plant resources offspring D5.
C4 plant of the present invention is barnyard grass.
Restorer R of the present invention is extensive 7109R or 7108R in Chongqing.
Maintainer B of the present invention is Chongqing 805B.
Beneficial effect of the present invention is: utilize biological technique method to extract the DNA of the barnyard grass that plant and leaf shape is good, blade is slightly involute in rice field, Chongqing region, by pollen tube passage method, the DNA of source far away resource between grass family genus is imported in Elite restorer line or maintainer, the beneficial genes such as straw specular removal, drought resisting, storage tolerance can be effectively utilized, expand the genetic distance of rice material, create excellent parent material, the special excellent combination of assembly; With the combination G46A/10486R that the restorer 10486R (change extensive 7109/ barnyard grass DNA) that the extensive 7109 importing barnyard grass STb gene in Elite restorer line Chongqing are bred as is measured with G46A, within 2014, carry out nonirrigated farmland plantation (transplanting after turning green no longer manual priming) under field conditions (factors), its ripening rate reaches 89%, and the paid per unit area yield in row district reaches 636.36 kilograms.The incubation of the excellent Combination nova such as G46A/10486R and application, effectively can reduce kind of a rice water consumption, saving water resource, preserve the ecological environment.
Embodiment
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed to the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, any simple modification done following examples according to technical spirit of the present invention or equivalent variations, all drop in protection scope of the present invention.
Embodiment 1
The selection of a kind of drought resisting hybrid rice, selecting that Chongqing local adaptation is strong, happiness is moist, like warm, drought-resistant C4 plant barnyard grass is donor, uses academy of agricultural sciences of Chongqing City " change excellent " change extensive 7109R, 7108 R and maintainer 805B of restorer that the comprehensive agronomy proterties that is bred as of paddy rice team is excellent are receptor parent;
(1) barnyard grass Exogenous DNA transfered: the First Year spring utilize pollen tube pathway to be imported to by barnyard grass STb gene in Chongqing Chongqing is extensive 7109, in extensive 7108, the maintainer Chongqing 805B of changing;
(2) barnyard grass DNA imports observation and the selection of progeny material: the First Year autumn carries out plantation and individual plant sowing (D1) in Hainan to the progeny material imported; The Second Year spring carries out the capable district of individual plant, and to plant each individual plant kind 10-15 capable and mix receipts (D2 generation all stable) respectively to individual plant in Chongqing; The Second Year autumn is each single-strain planting 20 row in Hainan, and carries out single receipts (D3) to the variant of the proterties inclined donor barnyard grasses such as blade profile, fringe type and plant type; 3rd year spring carried out the observation of row district in Chongqing to selected strain, and chose the excellent individual plant of Comprehensive Traits (D4) and carry out the general detection of DNA fingerprint figure; Choose the excellent maintainer of Comprehensive Traits and 650A etc. to carry out backcrossing making and protect, from detecting, the 3rd year autumn had that the selection list selecting economical character excellent barnyard grass resource offspring (D5) row district is received, maintainer proceeds the system that backcrosses and protects in Hainan simultaneously;
Lab simulation drought resisting qualification and the qualification of outdoor Water and Dry cultivation are carried out to selected material.
A, lab simulation drought resisting qualification:
To selected material in laboratory under 26 DEG C of conditions, compare with water (CK) with 15% polyethylene glycol (6000), seed germination under artificial drought conditions, filters out the material of the bud phase drought resisting standard that reaches (relative bud is long by≤40%, embryo root length≤75%, relatively bud do heavy≤45% relatively).
B, field test:
Paddy field and nonirrigated farmland natural conditions field plot test are carried out to selected material; All material is in nonirrigated farmland program request, and from being seeded into for six leaf phases, field keeps moisture state, does not rewater, then thinning (only staying a paddy rice shoot) after six leaf phases, and worm is controlled in field management, fertilising, weeding are the same with land for growing field crops.The material that after ripe, results do not lodge, filters out ripening rate height (≤50%), do not lodge, blast resisting material.Or all material sowing, transplant in paddy field, survive after rear water falls to doing naturally, throughout the growing season do not pour water always.Synthesis result therefrom filters out 7 parts of water, nonirrigated farmland ripening rate reach more than 80% drought resisting material, wherein 10486 (R7109 × barnyard grasses), 21288 (R7108 × barnyard grasses), 21281 (R7108 × barnyard grasses), 819A (650A/805B × barnyard grass) etc. are the progeny material importing edge barnyard grass resource far away respectively.
Embodiment 2
Autumn in 2013 imports the incubation of the barnyard grass matter source DNA restorer such as restorer 21281 (R7108 × barnyard grass), 21288 (R7108 × barnyard grasses), 10486 (R7109 × barnyard grasses) in Hainan is measured with Chongqing 650A, the 802A that changes, the male sterile lines such as 805A that change.
Spring in 2014 plants in Chongqing Agricultural Academy of Science Yu Xi science and technology demonstration district, Yongchuan District, Chongqing: in sowing on March 6, mulch film watery ricenursery, transplantation of seedlings in April 17, and planting specification is 33cm × 20cm, and each combination plants that 5-10 is capable, every row 12 nest.To transplant in latter 10 days field to fill with shallow water and be beneficial to rice shoot and be revival from transplanting, rice shoot survives after draining to the ripe field no longer manual priming of paddy rice; Before rice harves, each combination is averaged 5 nest investigation Spike granule character of spike number, by test, has therefrom filtered out the combination of the ripening rates such as 650A/21281R, 803A/21288R, 805A/10486 more than 85%.
Claims (4)
1. a selection for drought resisting hybrid rice, is characterized in that: comprise the following steps:
1) barnyard grass STb gene is imported in restorer R, maintainer B;
2) plant importing DNA progeny material, individual plant sowing obtains D1 seed rice;
3) each individual plant kind 10 ~ 15 row of individual plant capable district plantation D1, and individual plant is mixed respectively gather in the crops to obtain D2 seed rice;
4) 20 row are planted to each individual plant kind of D2, individual plant sowing is carried out to the variant of the inclined donor barnyard grass of blade profile, fringe type and characters with plant and obtains D3 seed rice;
5) general planting D3 individual plant kind, the individual plant that screening acquired character is excellent obtains D4 seed rice;
6) choose maintainer and 650A backcrosses to D4, obtain there is C4 plant resources offspring D5.
2. the selection of drought resisting hybrid rice according to claim 1, is characterized in that: described C4 plant is barnyard grass.
3. the selection of drought resisting hybrid rice according to claim 1, is characterized in that: described restorer R is extensive 7109R or 7108R in Chongqing.
4. the selection of drought resisting hybrid rice according to claim 1, is characterized in that: described maintainer B is Chongqing 805B.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410766615.5A CN104429934A (en) | 2014-12-12 | 2014-12-12 | Method for breeding drought-resistant hybrid rice |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782479A (en) * | 2015-04-01 | 2015-07-22 | 重庆市农业科学院 | Method for breeding horticulture type clustered floret rice material |
| CN106069453A (en) * | 2016-07-19 | 2016-11-09 | 莆田秀屿区群韬农业技术开发有限公司 | The breeding method of a kind of drought resisting rice and implantation methods |
| CN106688874A (en) * | 2016-11-28 | 2017-05-24 | 安徽华安种业有限责任公司 | Method used for rapidly cultivating novel drought durable type rice product |
| CN106718828A (en) * | 2016-11-28 | 2017-05-31 | 安徽华安种业有限责任公司 | A kind of breeding method of drought-enduring type rice varieties |
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
| CN111631132A (en) * | 2020-07-16 | 2020-09-08 | 黑龙江省农业科学院绥化分院 | Breeding method of herbicide-resistant dry-planting rice variety |
| CN114651686A (en) * | 2022-04-11 | 2022-06-24 | 海南省农业科学院粮食作物研究所 | Method for breeding new red rice variety by utilizing medicinal wild rice |
-
2014
- 2014-12-12 CN CN201410766615.5A patent/CN104429934A/en active Pending
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| 余新桥等: "节水抗旱杂交稻的选育和应用前景", 《分子植物育种》 * |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782479A (en) * | 2015-04-01 | 2015-07-22 | 重庆市农业科学院 | Method for breeding horticulture type clustered floret rice material |
| CN106069453A (en) * | 2016-07-19 | 2016-11-09 | 莆田秀屿区群韬农业技术开发有限公司 | The breeding method of a kind of drought resisting rice and implantation methods |
| CN106069453B (en) * | 2016-07-19 | 2019-06-04 | 新昌县大船畈生物科技有限公司 | A kind of breeding method and implantation methods of drought resisting rice |
| CN106688874A (en) * | 2016-11-28 | 2017-05-24 | 安徽华安种业有限责任公司 | Method used for rapidly cultivating novel drought durable type rice product |
| CN106718828A (en) * | 2016-11-28 | 2017-05-31 | 安徽华安种业有限责任公司 | A kind of breeding method of drought-enduring type rice varieties |
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
| CN111631132A (en) * | 2020-07-16 | 2020-09-08 | 黑龙江省农业科学院绥化分院 | Breeding method of herbicide-resistant dry-planting rice variety |
| CN114651686A (en) * | 2022-04-11 | 2022-06-24 | 海南省农业科学院粮食作物研究所 | Method for breeding new red rice variety by utilizing medicinal wild rice |
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Inventor after: Li Jingyong Inventor after: Tang Yongqun Inventor after: Zhang Xianwei Inventor after: Wen Ming Inventor after: Yao Xiong Inventor after: Xiao Renpeng Inventor after: Gan Xingyou Inventor before: Li Jingyong Inventor before: Tang Yongqun Inventor before: Zhang Xianwei Inventor before: Wen Ming Inventor before: Yao Xiong Inventor before: Xiao Renpeng Inventor before: Gan Xingyou |
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Application publication date: 20150325 |
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