CN108476975A - One cultivates peanut emasculating method - Google Patents
One cultivates peanut emasculating method Download PDFInfo
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- CN108476975A CN108476975A CN201810332471.0A CN201810332471A CN108476975A CN 108476975 A CN108476975 A CN 108476975A CN 201810332471 A CN201810332471 A CN 201810332471A CN 108476975 A CN108476975 A CN 108476975A
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- emasculation
- peanut
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- petal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides one and cultivates peanut emasculating method, includes the following steps：(1) emasculation solution is prepared, which contains the sucrose of the Boratex and 1wt% 8wt% of 0.01wt% 0.2wt%, remaining is water；(2) heating emasculation solution extremely kills pollen and does not damage the temperature of column cap, is then poured into the cool-bag inside holding equipped with cotton balls；(3) petal is removed：Remove 2 peanut petals；(4) emasculation：The cotton pellet for being soaked with emasculation solution is gently placed on tweezers and is removed in the spending of petal, 5 10min is placed, takes out cotton balls.It is peanut emasculating method saving of labor provided by the invention, time saving, simple, the method for removing anther is pressed from both sides using tweezers, one skilled worker one day about can be 40 50 with emasculation, and 1.6 2.5 times can be improved with 80 100 flowers of emasculation, efficiency using method 1 skilled worker that is averaged of the present invention.Gametocide is thorough, does not hinder column cap, and hybridization success rate is high, and this method hybridizes success rate 20% 25%, and true cenospecies rate is 75% 80%, compares traditional emasculating method, and hybridization success rate improves 67% 150%, and true cenospecies rate improves 12% 23%.
Description
Technical field
The invention belongs to peanut Biotechnology in Genetic Breeding fields, and in particular to one cultivates peanut emasculating method.
Background technology
Peanut is one of most important oil crops in China, about 70,000,000 mu of annual planting area, peanut because its high yield and high quality,
Efficient economic benefit becomes China's per unit area yield, total yield and the highest oil crops of foreign exchange earning and industrial crops, is that China is high-quality
The important sources of edible vegetable oil and high-quality food plant albumen occupy importantly in food security in China and grease safety
Position.New peanut variety cultivation is to ensure China's Peanut Industry Development premise, and China's new peanut variety cultivates main use at this stage
Sexual hybridization method, under the premise of peanut does not find male sterile, emasculation is to hybridize the pass of success or failure in peanut hybridization process
Key.
Present peanut emasculation mainly still utilizes the method for manually using tweezers, slowly extracing anther.Concrete operations are：Choosing
The bud of yellow petal, i.e., the flower that the next morning can open, left-handed thumb and middle finger are manifested with calyx fine fisssure
The base portion of bud is pinched, the right hand holds tweezers and gently pushes calyx, vexil, ala aside, is pushed down in left-handed index finger and thumb
The petal pushed aside at the curved back of the body for then gently pressing fossil fragments with tweezers, makes pistil expose, and is extractd the anther of 8 stamens with tweezers
Totally, then with finger fossil fragments are pushed back to original position, vexil, ala is made to restore to the original state.Since peanut plant is short, portion of blooming
Position is low, and floral organ is complicated, and this method emasculation is time-consuming, is also easy to hurt column cap.Hybridized using this emasculating method
Success rate is 10%-12%, and true cenospecies rate is 65%-67%, so improving peanut emasculating method is very important.
Invention content
It is time-consuming for current peanut emasculating method, it is easy to hurt column cap, and hybridize that success rate is low, true cenospecies
The low above problem of rate, the present invention provide one and cultivate peanut emasculating method, and this method is time saving, saving of labor, simple, and emasculation effect is good.
The present invention is achieved by the following technical solutions：
One cultivates peanut emasculating method, includes the following steps：
(1) emasculation solution is prepared, which contains the Boratex of 0.01wt%-0.2wt% and the sugarcane of 1wt%-8wt%
Sugar, remaining is water；
(2) heating emasculation solution extremely kills pollen and does not damage the temperature of column cap, is then poured into the guarantor equipped with cotton balls
Warm container inside holding；
(3) petal is removed：Remove 2 peanut petals；
(4) emasculation：The cotton pellet for being soaked with emasculation solution is gently placed on tweezers and is removed in the spending of petal, 5- is placed
10min takes out cotton balls.
Emasculation solution contains boron element, can promote the development of column cap；After sucrose is added in water, the ratio of solution can be reduced
Emasculation solution can be heated to required temperature by heat in a relatively short period of time.
Further, the step (1), the emasculation solution contain the Boratex of 0.1wt% and the sugarcane of 3wt%-5wt%
Sugar.
Further, the temperature of step (2) the emasculation solution is 44-45 DEG C.
The temperature of emasculation solution can be adjusted according to environment temperature, if environment temperature height, the temperature of emasculation solution
It can suitably reduce, conversely, can properly increase.
Advantageous effect：
(1) peanut emasculating method saving of labor provided by the invention, time saving, simple, the method for removing anther using tweezers folder, one
Skilled worker one day about can be 40-50 with emasculation, and being averaged 1 skilled worker using the method for the present invention can be 80-100 with emasculation
Flower, efficiency improve 1.6-2.5 times.
(2) gametocide is thorough, does not hinder column cap, hybridization success rate is high, and this method hybridizes success rate 20%-25%, true cenospecies
Rate is 75%-80%, compares traditional emasculating method, and hybridization success rate improves 67%-150%, and true cenospecies rate improves 12%-
23%.
Specific implementation mode
The present invention is described in further details with reference to specific embodiment.
Embodiment 1
The aqueous solution of the sucrose of the Boratex and 3wt% that contain 0.1wt% is prepared as emasculation solution；Add before emasculation
Then hot emasculation solution is poured into thermal insulation cup or thermos flask equipped with cotton pellet and preserves to 44-45 DEG C, spare；Remove 2 film clips
Raw petal is gently placed on the cotton pellet for being soaked with emasculation solution with tweezers and has removed in the spending of 2 petals, places 8min, take
Go out cotton balls and peanut emasculation work can be completed.
Embodiment 2
The aqueous solution of the sucrose of the Boratex and 5wt% that contain 0.1wt% is prepared as emasculation solution；Add before emasculation
Then hot emasculation solution is poured into thermal insulation cup or thermos flask equipped with cotton pellet and preserves to 44-45 DEG C, spare；Remove 2 film clips
Raw petal is gently placed on the cotton pellet for being soaked with emasculation solution with tweezers and has removed in the spending of 2 petals, places 8min, take
Go out cotton balls and peanut emasculation work can be completed.
Embodiment 3
The aqueous solution of the sucrose of the Boratex and 8wt% that contain 0.2wt% is prepared as emasculation solution；Add before emasculation
Then hot emasculation solution is poured into thermal insulation cup or thermos flask equipped with cotton pellet and preserves to 44-45 DEG C, spare；Remove 2 film clips
Raw petal is gently placed on the cotton pellet for being soaked with emasculation solution with tweezers and has removed in the spending of 2 petals, places 5min, take
Go out cotton balls and peanut emasculation work can be completed.
Embodiment described above is only that the preferred embodiment of the present invention is described, not to the scope of the present invention
It is defined, under the premise of not departing from design spirit of the present invention, those of ordinary skill in the art are to technical scheme of the present invention
The various modifications made and improvement should all be fallen into the protection domain of claims of the present invention determination.
Claims (3)
1. emasculating method of cultivating peanut, which is characterized in that include the following steps：
(1) emasculation solution is prepared, which contains the Boratex of 0.01wt%-0.2wt% and the sucrose of 1wt%-8wt%,
Yu Weishui；
(2) heating emasculation solution extremely kills pollen and does not damage the temperature of column cap, is then poured into the heat preservation equipped with cotton balls and holds
Device inside holding；
(3) petal is removed：Remove 2 peanut petals；
(4) emasculation：The cotton pellet for being soaked with emasculation solution is gently placed on tweezers and is removed in the spending of petal, 5-10min is placed, takes
Go out cotton balls.
2. peanut emasculating method according to claim 1, which is characterized in that the step (1), the emasculation solution contain
The Boratex of 0.1wt% and the sucrose of 3wt%-5wt%.
3. peanut emasculating method according to claim 1, which is characterized in that the temperature of step (2) the emasculation solution is
44-45℃。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810332471.0A CN108476975A (en) | 2018-04-13 | 2018-04-13 | One cultivates peanut emasculating method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810332471.0A CN108476975A (en) | 2018-04-13 | 2018-04-13 | One cultivates peanut emasculating method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108476975A true CN108476975A (en) | 2018-09-04 |
Family
ID=63315855
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810332471.0A Pending CN108476975A (en) | 2018-04-13 | 2018-04-13 | One cultivates peanut emasculating method |
Country Status (1)
| Country | Link |
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| CN (1) | CN108476975A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101493A (en) * | 1993-10-13 | 1995-04-19 | 张力田 | Rice breeding technology of improving seed properties |
| CN1418536A (en) * | 2002-11-13 | 2003-05-21 | 中国科学院长春地理研究所 | Simple and high efficiency device for castration for hybridization of rice |
| CN101647387A (en) * | 2009-08-13 | 2010-02-17 | 陈启康 | Distant hybridization breeding method among three categories of shoal spartina alterniflora loisel, maize and rice |
| CN102499057A (en) * | 2011-10-26 | 2012-06-20 | 龚辉 | Cotton castration hybrid seed production method |
| CN103583246A (en) * | 2013-11-25 | 2014-02-19 | 合江县彭家河荔枝专业合作社 | Method for fast improving survival rate for hybridization of longan and litchi |
| CN103875495A (en) * | 2014-02-08 | 2014-06-25 | 苏州市阳澄湖现代农业发展有限公司 | Close-planting high-yield cultivation method for early bearing walnuts |
| CN106508670A (en) * | 2017-01-06 | 2017-03-22 | 商丘市农林科学院 | Method for improving peanut hybridization efficiency |
| CN107034230A (en) * | 2017-06-01 | 2017-08-11 | 山西省农业科学院经济作物研究所 | A kind of ultrasonic-assisted pollen mediated plant genetic transformation method |
-
2018
- 2018-04-13 CN CN201810332471.0A patent/CN108476975A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101493A (en) * | 1993-10-13 | 1995-04-19 | 张力田 | Rice breeding technology of improving seed properties |
| CN1418536A (en) * | 2002-11-13 | 2003-05-21 | 中国科学院长春地理研究所 | Simple and high efficiency device for castration for hybridization of rice |
| CN101647387A (en) * | 2009-08-13 | 2010-02-17 | 陈启康 | Distant hybridization breeding method among three categories of shoal spartina alterniflora loisel, maize and rice |
| CN102499057A (en) * | 2011-10-26 | 2012-06-20 | 龚辉 | Cotton castration hybrid seed production method |
| CN103583246A (en) * | 2013-11-25 | 2014-02-19 | 合江县彭家河荔枝专业合作社 | Method for fast improving survival rate for hybridization of longan and litchi |
| CN103875495A (en) * | 2014-02-08 | 2014-06-25 | 苏州市阳澄湖现代农业发展有限公司 | Close-planting high-yield cultivation method for early bearing walnuts |
| CN106508670A (en) * | 2017-01-06 | 2017-03-22 | 商丘市农林科学院 | Method for improving peanut hybridization efficiency |
| CN107034230A (en) * | 2017-06-01 | 2017-08-11 | 山西省农业科学院经济作物研究所 | A kind of ultrasonic-assisted pollen mediated plant genetic transformation method |
Non-Patent Citations (2)
| Title |
|---|
| 张祖明，路兆英: "影响花生杂交成活率的几个气象因子分析 ", 《花生科技》 * |
| 徐庆年等: "花生有性杂交技术的改进及几点看法 ", 《花生学报》 * |
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Application publication date: 20180904 |