CN102783408A - Method for killing male of japonica rice by warm water - Google Patents

Method for killing male of japonica rice by warm water Download PDF

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Publication number
CN102783408A
CN102783408A CN2012102953421A CN201210295342A CN102783408A CN 102783408 A CN102783408 A CN 102783408A CN 2012102953421 A CN2012102953421 A CN 2012102953421A CN 201210295342 A CN201210295342 A CN 201210295342A CN 102783408 A CN102783408 A CN 102783408A
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male
rice
warm water
flower
female parent
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CN102783408B (en
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余波
林添资
景德道
龚红兵
周义文
盛生兰
刁立平
李闯
钱华飞
曾生元
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JINAN GAORUI BIOTECHNOLOGY Co.,Ltd.
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JIANGSU FENGYUAN SEED Co Ltd
Zhenjiang Institute of Agricultural Sciences Jiangsu Hilly Area
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Abstract

The invention belongs to the warm water male-killing technical field of japonica rice, and provides a method for killing male of japonica rice by warm water. The method comprises the steps of digging out to-be-hybridized female parents in the afternoon of the previous day of blooming; cutting too old and too tender tillers; removing bloomed glumous flowers and glumous flowers with tender base part; selecting glumous flowers which will bloom in 2-3 days, directly cutting 1/2 of the glumous flowers; in the morning of day for pollination, preparing one pot of warm water at 45 DEG C; soaking the cut japonica rice ears in the warm water at 45 DEG C in about 2 hours before the female parent blooms normally; after 3-5min, taking out the ears, and flicking extended anthers to enable stigma to be bared, wherein the anthers extend successively; covering the female parent with kraft paper bag after male-killing, transfering the female parent to place near male parent, and taking the male parent to pollinate when blooming; grouping the pollinated female parents for transplanting; cutting the hybrid seeds when maturing. The method for killing male of japonica rice by warm water breaks through hybridizing time limit of the japonica rice, increases hybridization efficiency, has good pollination effect, improves outcrossing maturing rate, is low in glumous flower-cutting technology demand and kills male thoroughly.

Description

A kind of japonica rice lukewarm water kills male method
Technical field
The invention belongs to the japonica rice lukewarm water and kill male technical field, relate in particular to a kind of japonica rice lukewarm water and kill male method.
Background technology
The hybridization of paddy rice can be divided on the step kills male and pollination, and the male method of killing that adopts of breeding units has much at present, cuts grain husk and chooses that medicine method, chemical emasculation method, vacuum castration method, hot gas are killed male method, lukewarm water kills male method.
Lukewarm water kills hero owing to simple to operate, low for equipment requirements, receives the welcome of breeding units most, and method is to pollinate the same day, handles spike of rice 5 minutes for 45 ℃ with warm water, can reach the purpose of killing hero; Its shortcoming is to receive time restriction, and a people has only the time in 3-4 hour morning to cut grain husk every day, has limited the pace of progress of paddy rice cross breeding breeding.
Summary of the invention
The invention provides a kind of japonica rice lukewarm water and kill male method; The lukewarm water that being intended to solve prior art provides kills male method and receives time restriction to a great extent; A people has only the time in 3-4 hour morning to cut grain husk every day; Limited the pace of progress of paddy rice cross breeding breeding, production efficiency is lower, the problem that labour intensity is bigger.
The object of the present invention is to provide a kind of japonica rice lukewarm water to kill male method, this method may further comprise the steps:
Step 1; The female parent band soil of noon before that day needs being done hybridization of blooming digs out, and cuts off old and tender excessively tillering, and keeps the spike of rice of opening grain husk 1/3 to 2/3; Reject grain husk flower and the tender excessively grain husk flower of base portion opened; The grain husk that in 2-3 days, can bloom selected and remain is spent and is directly cut grain husk 1/2, and the plant that shears is left in the adapt circumstance, and the fringe head sprays in right amount and shines clear water and preserves moisture;
Step 2 prepares the warm water of 45 ℃ in a kettle at pollination morning on the same day, and normally bloom at female parent preceding about 2 hours, the spike of rice that shears immersed in 45 ℃ of warm water take out after 3-5 minute, flower pesticide extends successively, flicks the flower pesticide of elongation, makes column cap exposed, is convenient to pollination;
Step 3 puts kraft paper bag with the female parent that kills behind the hero, moves near the male parent, gets the male parent pollination when containing flower;
Step 4 is concentrated the female parent after the pollination and is planted together, so that management is made regular check on simultaneously, performs insect protected, mildew-resistant work, when crossbreed is ripe, cuts receipts.
Further, in step 1, the extremely male female parent of selection is a japonica rice.
Further, in step 1, cut grain husk and must reject the grain husk flower of having opened clean before.
Further, in step 2, took out back flower pesticide in 3-5 minute and do not extend if the spike of rice that shears immersed in 45 ℃ of warm water, can be again with spike of rice as in 40 ℃ the hot gas bottle 5-8 minute, stimulate the flower pesticide elongation.
Further, in step 2, kill male temperature and be controlled between 43-45 ℃, the time was controlled at 3-5 minute, killed male visual kind of temperature and time and weather condition appropriateness simultaneously and changed.
Further, the time of killing hero will be controlled at the hybridization female parent and bloom preceding about 2 hours, is convenient to stimulate the flower pesticide elongation.
Japonica rice lukewarm water provided by the invention kills male method; The female parent of needs being done hybridization in the noon before that day of blooming digs out; Cut off old and tender excessively tillering, and rejected the grain husk flower and the tender excessively grain husk flower of base portion opened, the grain husk that in 2-3 days, can bloom selected and remain is spent and is directly cut clever 1/2; In pollination morning on the same day, prepare the warm water of 45 ℃ in a kettle, normally bloom at female parent preceding about 2 hours, the spike of rice that shears immersed in 45 ℃ of warm water take out after 3-5 minute, flower pesticide extends successively, flicks the flower pesticide of elongation, makes column cap exposed outside, is convenient to pollination; The female parent that kills behind the hero is put kraft paper bag, move near the male parent, get the male parent pollination when containing flower; Female parent after the pollination concentrated plant together,, perform insect protected, mildew-resistant work, when crossbreed is ripe, cut receipts so that management is made regular check on simultaneously; This japonica rice lukewarm water kills the restriction that male method has broken through the japonica rice hybridization time, has improved hybridization efficiency, and it is effective to pollinate; Outcrossing seed-setting rate improves; Compare with the glume-cutting, anther-cutting, male-killing method, low to cutting clever specification requirement,, glume-cutting, anther-cutting, male-killing method male extremely with lukewarm water compared; It is more thorough to kill hero, has stronger popularization and using value.
Description of drawings
Fig. 1 is that the japonica rice lukewarm water that the embodiment of the invention provides kills the realization flow figure of male method.
Embodiment
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further specified below in conjunction with accompanying drawing and embodiment.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in the qualification invention.
The japonica rice lukewarm water that Fig. 1 shows the embodiment of the invention to be provided kills the realization flow of male method.
This method may further comprise the steps:
In step S101; The female parent band soil of noon before that day needs being done hybridization of blooming digs out, and cuts off old and tender excessively tillering, and keeps the spike of rice of opening grain husk 1/3 to 2/3; Reject grain husk flower and the tender excessively grain husk flower of base portion opened; The grain husk that in 2-3 days, can bloom selected and remain is spent and is directly cut grain husk 1/2, and the plant that shears is left in the adapt circumstance, and the fringe head sprays in right amount and shines clear water and preserves moisture;
In step S102, pollinated morning on the same day, prepare the warm water of 45 ℃ in a kettle; Normally bloom at female parent preceding about 2 hours, the spike of rice that shears immersed in 45 ℃ of warm water take out after 3-5 minute, flower pesticide extends successively; Flick the flower pesticide of elongation, make column cap exposed, be convenient to pollination;
In step S103, the female parent that kills behind the hero is put kraft paper bag, move near the male parent, get the male parent pollination when containing flower;
In step S104, the female parent after the pollination concentrated plant together, so that management is made regular check on simultaneously, perform insect protected, mildew-resistant work, when crossbreed is ripe, cut receipts.
In embodiments of the present invention, in step S101, the extremely male female parent of selection is a japonica rice.
In embodiments of the present invention, in step S101, cut grain husk and must reject the grain husk flower of having opened clean before.
In embodiments of the present invention, in step S102, took out back flower pesticide in 3-5 minute and do not extend if the spike of rice that shears immersed in 45 ℃ of warm water, can be again with spike of rice as in 40 ℃ the hot gas bottle 5-8 minute, stimulate the flower pesticide elongation.
In embodiments of the present invention, in step S102, kill male temperature and be controlled between 43-45 ℃, the time was controlled at 3-5 minute, killed male visual kind of temperature and time and weather condition appropriateness simultaneously and changed.
In embodiments of the present invention, the time of killing hero will be controlled at the hybridization female parent and bloom preceding about 2 hours, is convenient to stimulate the flower pesticide elongation.
Below in conjunction with accompanying drawing and specific embodiment application principle of the present invention is further described.
The objective of the invention is to solve the problems and shortcomings that exist in the prior art, advance to kill male technology fast development, the japonica rice lukewarm water that the embodiment of the invention provides kills male method and may further comprise the steps:
A) the female parent band soil of noon before that day needs being done hybridization of blooming digs out; Cut off old and tender excessively tillering; Keep the spike of rice open grain husk 1/3 to 2/3, reject the grain husk flower and the tender excessively grain husk flower of base portion opened, the grain husk that in 2-3 days, can bloom selected and remain is spent and is directly cut clever 1/2; The plant that shears is left in the adapt circumstance, and the fringe head sprays the solarization clear water in right amount preserves moisture;
B) prepared the warm water of 45 ℃ in a kettle pollination morning on the same day, normally bloom at female parent preceding about 2 hours; The spike of rice that shears immersed in 45 ℃ of warm water takes out after 3-5 minute, flower pesticide extend successively (if flower pesticide does not extend, can be again with spike of rice as in 40 ℃ the hot gas bottle 5-8 minute; Stimulate the flower pesticide elongation); Flick the flower pesticide of elongation, make column cap exposed, be convenient to pollination;
The female parent that C) will kill behind the hero puts kraft paper bag, moves near the male parent, gets the male parent pollination when containing flower;
D) female parent after will pollinating is concentrated and is planted together, so that management is made regular check on simultaneously, performs insect protected, mildew-resistant work, when crossbreed is ripe, cuts receipts.
Be for No. 14 example with town rice 88 and town rice below, this japonica rice lukewarm water of simple declaration kills the specific embodiment step of male method:
Town rice 88 is an anti-bar breeding in early days of Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute, precocious japonica rice variety.
The town rice is for No. 14 that article are of fine quality, output is high, the japonica rice variety of better resistance for of newly breeding in Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute.
Cross method:
A) select to have high-quality, anti-stripe virus disease, yielding ability is good, colony self regulating power strong, late ripe in round-grained rice town rice No. 14 maternal for hybridization, good, ripe mutually good, the strong root of the leaf attitude of selected plant strengthen stalk, bud gesture strong, press down rice 88 and make male parent.
B) afternoon August 26, will press down No. 14 companies of rice soil and dig out, pruned the branch evil that old Buddhist monk is not eared, select and remain and open the spike of rice of clever 1/3-2/3, prune to have opened and spend with tender excessively Ying, remaining Ying spends and directly cuts 1/2 Ying's shell, treats to kill in second day male the processing.
C) beginning in the mornings 9: 30 on August 27; The fringe head of the maternal town of the hybridization of cutting Ying rice No. 14 poured in 45 ℃ the warm-water bottle and to take out in 3-5 minute; Flower pesticide extends successively, flicks flower pesticide, and the hot gas bottle that the fringe head that after half an hour flower pesticide is not extended inserts in about 40 degree took out in 5-8 minute; If do not extend flower pesticide in addition, then directly wipe out.
The female parent town rice that D) will kill behind the hero puts kraft paper bag No. 14, moves near the male parent town rice 88, waits to press down and gets the fringe pollination when rice 88 is contained flower.
E) maternal the concentrating of the hybridization after will pollinating planted together, so that management is made regular check on simultaneously, regularly sprays agricultural chemicals, performs insect protected, mildew-resistant work, 9/18, cuts fringe and receives kind.
Above steps summarize is: August 26, following westerly zone soil dug out town rice 461, and cut clever shell 1/2; Morning August 27,45 ℃ of warm water killed male 3-5 minute, the flower pesticide bullet that extends flown, and bagging, town rice 88 is the male parent pollination; Received September 19 and plant.
The present invention has following beneficial effect:
1. break through the restriction of japonica rice hybridization time, improved hybridization efficiency
It is to cut grain husk the same day in pollination that conventional lukewarm water kills hero, and the method after the improvement can be cut grain husk in noon before that day and even evening, has prolonged greatly and has cut the grain husk time, and the hybridization quantity that the people did can double above.
2. it is effective to pollinate, and outcrossing seed-setting rate improves
It is to kill hero morning on the same day in pollination that common lukewarm water kills hero, because the maternal natural time of blooming of distance is also long, and the difficulty elongation of most of japonica rice flower pesticide, during pollination, flower pesticide stops up column cap, influences the male parent pollination; And the method after the improvement is killed in preceding 2 hours malely normal natural the blooming of female parent with warm water, can stimulate the elongation of maternal flower pesticide rapidly, as long as flick flower pesticide, just can make column cap exposed, has greatly improved the outcrossing seed-setting rate that male parent is pollinated.
3. compare with the glume-cutting, anther-cutting, male-killing method, low to cutting clever specification requirement
The glume-cutting, anther-cutting, male-killing method should be broken flower pesticide, can not damage column cap again, only need cut clever shell and the lukewarm water of improvement is male extremely, whether flower pesticide is not broken require.
4., glume-cutting, anther-cutting, male-killing method male extremely with lukewarm water compared, and hero is more thorough extremely.
After the warm water processing, most flower pesticide are killed, even a small amount of pollen has vigor, also eject clever shell after the flower pesticide elongation easily, and, glume-cutting, anther-cutting, male-killing method male extremely with lukewarm water compared, and pseudostationary is considerably less.
The japonica rice lukewarm water that the embodiment of the invention provides kills male method; The female parent of needs being done hybridization in the noon before that day of blooming digs out; Cut off old and tender excessively tillering, and rejected the grain husk flower and the tender excessively grain husk flower of base portion opened, the grain husk that in 2-3 days, can bloom selected and remain is spent and is directly cut clever 1/2; In pollination morning on the same day, prepare the warm water of 45 ℃ in a kettle, normally bloom at female parent preceding about 2 hours, the spike of rice that shears immersed in 45 ℃ of warm water take out after 3-5 minute, flower pesticide extends successively, flicks the flower pesticide of elongation, makes column cap exposed outside, is convenient to pollination; The female parent that kills behind the hero is put kraft paper bag, move near the male parent, get the male parent pollination when containing flower; Female parent after the pollination concentrated plant together,, perform insect protected, mildew-resistant work, when crossbreed is ripe, cut receipts so that management is made regular check on simultaneously; This japonica rice lukewarm water kills the restriction that male method has broken through the japonica rice hybridization time, has improved hybridization efficiency, and it is effective to pollinate; Outcrossing seed-setting rate improves; Compare with the glume-cutting, anther-cutting, male-killing method, low to cutting clever specification requirement,, glume-cutting, anther-cutting, male-killing method male extremely with lukewarm water compared; It is more thorough to kill hero, has stronger popularization and using value.
The above is merely preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of within spirit of the present invention and principle, being done, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.

Claims (6)

1. a japonica rice lukewarm water kills male method, it is characterized in that this method may further comprise the steps:
Step 1; The female parent band soil of noon before that day needs being done hybridization of blooming digs out, and cuts off old and tender excessively tillering, and keeps the spike of rice of opening grain husk 1/3 to 2/3; Reject grain husk flower and the tender excessively grain husk flower of base portion opened; The grain husk that in 2-3 days, can bloom selected and remain is spent and is directly cut grain husk 1/2, and the plant that shears is left in the adapt circumstance, and the fringe head sprays in right amount and shines clear water and preserves moisture;
Step 2 prepares the warm water of 45 ℃ in a kettle at pollination morning on the same day, and normally bloom at female parent preceding about 2 hours, the spike of rice that shears immersed in 45 ℃ of warm water take out after 3-5 minute, flower pesticide extends successively, flicks the flower pesticide of elongation, makes column cap exposed, is convenient to pollination;
Step 3 puts kraft paper bag with the female parent that kills behind the hero, moves near the male parent, gets the male parent pollination when containing flower;
Step 4 is concentrated the female parent after the pollination and is planted together, so that management is made regular check on simultaneously, performs insect protected, mildew-resistant work, when crossbreed is ripe, cuts receipts.
2. the method for claim 1 is characterized in that, in step 1, the extremely male female parent of selection is a japonica rice.
3. the method for claim 1 is characterized in that, in step 1, cuts grain husk and must reject the grain husk flower of having opened clean before.
4. the method for claim 1 is characterized in that, in step 2, takes out back flower pesticide in 3-5 minute and does not extend if the spike of rice that shears immersed in 45 ℃ of warm water, can be again with spike of rice as in 40 ℃ the hot gas bottle 5-8 minute, stimulate the flower pesticide elongation.
5. the method for claim 1 is characterized in that, in step 2, kills male temperature and is controlled between 43-45 ℃, and the time was controlled at 3-5 minute, kills male visual kind of temperature and time and weather condition appropriateness simultaneously and changes.
6. the method for claim 1 is characterized in that, the time of killing hero will be controlled at the hybridization female parent and bloom preceding about 2 hours, is convenient to stimulate the flower pesticide elongation.
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Publication number Priority date Publication date Assignee Title
CN103858748A (en) * 2012-12-12 2014-06-18 东港市农业技术推广中心 Breeding method of new rice variety
CN104885928A (en) * 2015-06-14 2015-09-09 安徽徽大农业有限公司 Rice hybridization and castration method
CN105123496A (en) * 2015-07-17 2015-12-09 浙江省农业科学院 Paddy rice hybridization method
CN106171945A (en) * 2016-07-11 2016-12-07 合肥扬扬农业科技有限公司 A kind of castration method for rice hyberdization
CN107360965A (en) * 2017-08-28 2017-11-21 贵州省农作物品种资源研究所 A kind of quick test cross compound formulation of rice
CN116439124A (en) * 2023-03-29 2023-07-18 永州市农业科学研究所 Water-immersed glume Quan Ying emasculation method and application thereof in rice crossbreeding

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103858748A (en) * 2012-12-12 2014-06-18 东港市农业技术推广中心 Breeding method of new rice variety
CN104885928A (en) * 2015-06-14 2015-09-09 安徽徽大农业有限公司 Rice hybridization and castration method
CN105123496A (en) * 2015-07-17 2015-12-09 浙江省农业科学院 Paddy rice hybridization method
CN106171945A (en) * 2016-07-11 2016-12-07 合肥扬扬农业科技有限公司 A kind of castration method for rice hyberdization
CN107360965A (en) * 2017-08-28 2017-11-21 贵州省农作物品种资源研究所 A kind of quick test cross compound formulation of rice
CN116439124A (en) * 2023-03-29 2023-07-18 永州市农业科学研究所 Water-immersed glume Quan Ying emasculation method and application thereof in rice crossbreeding

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