CN102783408B - Method for killing male of japonica rice by warm water - Google Patents
Method for killing male of japonica rice by warm water Download PDFInfo
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- CN102783408B CN102783408B CN201210295342.1A CN201210295342A CN102783408B CN 102783408 B CN102783408 B CN 102783408B CN 201210295342 A CN201210295342 A CN 201210295342A CN 102783408 B CN102783408 B CN 102783408B
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Abstract
The invention belongs to the warm water male-killing technical field of japonica rice, and provides a method for killing male of japonica rice by warm water. The method comprises the steps of digging out to-be-hybridized female parents in the afternoon of the previous day of blooming; cutting too old and too tender tillers; removing bloomed glumous flowers and glumous flowers with tender base part; selecting glumous flowers which will bloom in 2-3 days, directly cutting 1/2 of the glumous flowers; in the morning of day for pollination, preparing one pot of warm water at 45 DEG C; soaking the cut japonica rice ears in the warm water at 45 DEG C in about 2 hours before the female parent blooms normally; after 3-5min, taking out the ears, and flicking extended anthers to enable stigma to be bared, wherein the anthers extend successively; covering the female parent with kraft paper bag after male-killing, transfering the female parent to place near male parent, and taking the male parent to pollinate when blooming; grouping the pollinated female parents for transplanting; cutting the hybrid seeds when maturing. The method for killing male of japonica rice by warm water breaks through hybridizing time limit of the japonica rice, increases hybridization efficiency, has good pollination effect, improves outcrossing maturing rate, is low in glumous flower-cutting technology demand and kills male thoroughly.
Description
Technical field
The invention belongs to japonica rice lukewarm water gametocide technical field, particularly relate to a kind of method of japonica rice lukewarm water gametocide.
Background technology
The hybridization of paddy rice can be divided into gametocide and pollination step, and the gametocide method that current breeding units adopts has a lot, cuts grain husk and chooses medicine method, chemical emasculation method, vacuum emasculation method, hot gas gametocide method, lukewarm water gametocide method.
Lukewarm water gametocide is due to simple to operate, low for equipment requirements, is subject to the welcome of breeding units most, and method is being pollinated the same day, processes spike of rice 5 minutes, can reach the object of gametocide with 45 DEG C, warm water; Its shortcoming is by time restriction, and a people only has the time in 3-4 hour morning to cut grain husk every day, limits the pace of progress of paddy rice cross breeding breeding.
Summary of the invention
The invention provides a kind of method of japonica rice lukewarm water gametocide, the method being intended to solve the lukewarm water gametocide that prior art provides is subject to time restriction to a great extent, a people only has the time in 3-4 hour morning to cut grain husk every day, limit the pace of progress of paddy rice cross breeding breeding, production efficiency is lower, the problem that labour intensity is larger.
The object of the present invention is to provide a kind of method of japonica rice lukewarm water gametocide, the method comprises the following steps:
Step one, bloom and dig out needing to do the female parent band soil of hybridizing noon before that day, cut off old and excessively tender tillering, retain the spike of rice opening grain husk 1/3 to 2/3, reject the grain husk that grain husk is spent and base portion is the excessively tender flower opened, the grain husk that can bloom in 2-3 days selected and remain is spent and is directly cut grain husk 1/2, leaves in adapt circumstance by the plant sheared, and fringe head sprays in right amount and shines clear water moisturizing;
Step 2, pollinated morning on the same day, prepared the warm water of 45 DEG C, a kettle, and at front 2 hours that female parent is normally bloomed, immersed by the spike of rice sheared in 45 DEG C of warm water and take out after 3-5 minute, flower pesticide extends successively, flicks the flower pesticide of elongation, makes column cap exposed, is convenient to pollination;
Step 3, puts kraft paper bag by the female parent after gametocide, moves near male parent, gets male parent pollination when containing flower;
Step 4, concentrates the female parent after pollination and plants together, so that management, make regular check on simultaneously, perform insect protected, mildew-resistant work, cuts receipts when crossbreed is ripe.
Further, in step one, the gametocide female parent of selection is japonica rice.
Further, in step one, must the grain husk flower opened be rejected clean before cutting grain husk.
Further, in step 2, after within 3-5 minute, taking out if to be immersed by the spike of rice sheared in 45 DEG C of warm water, flower pesticide does not extend, can again by spike of rice as 5-8 minute in the hot gas bottle of 40 DEG C, stimulate flower pesticide to extend.
Further, in step 2, the temperature of gametocide controls between 43-45 DEG C, and time controling is at 3-5 minute, and simultaneously the visual kind of temperature and time of gametocide and weather condition appropriateness change.
Further, the time of gametocide will control 2 hours before hybridization female parent is bloomed, and is convenient to stimulate flower pesticide to extend.
The method of japonica rice lukewarm water gametocide provided by the invention, one afternoon needs are done the female parent of hybridizing before flowering to dig out, cut off old and excessively tender tillering, and rejected the grain husk flower opened and the excessively tender grain husk flower of base portion, the grain husk that can bloom in 2-3 days selected and remain is spent and is directly cut clever 1/2; Pollinating morning on the same day, prepare the warm water of 45 DEG C, a kettle, at front 2 hours that female parent is normally bloomed, immersed by the spike of rice sheared in 45 DEG C of warm water and take out after 3-5 minute, flower pesticide extends successively, flicks the flower pesticide of elongation, makes column cap outside exposed, is convenient to pollination; Female parent after gametocide is put kraft paper bag, moves near male parent, when containing flower, get male parent pollination; Female parent after pollination is concentrated and plants together, so that management, make regular check on simultaneously, perform insect protected, mildew-resistant work, cut receipts when crossbreed is ripe; The method of this japonica rice lukewarm water gametocide breaches the restriction of japonica rice hybridization time, improve hybridization efficiency, Pollination Effect is good, outcrossing seed-setting rate improves, compare with glume-cutting, anther-cutting, male-killing method, to cutting, clever technical requirement is low, compares with lukewarm water gametocide, glume-cutting, anther-cutting, male-killing method, gametocide is more thorough, has stronger propagation and employment and is worth.
Accompanying drawing explanation
Fig. 1 is the realization flow figure of the method for the japonica rice lukewarm water gametocide that the embodiment of the present invention provides.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is described in further detail.Should be appreciated that specific embodiment described herein only in order to explain the present invention, and be not used in restriction invention.
Fig. 1 shows the realization flow of the method for the japonica rice lukewarm water gametocide that the embodiment of the present invention provides.
The method comprises the following steps:
In step S101, bloom and dig out needing to do the female parent band soil of hybridizing noon before that day, cut off old and excessively tender tillering, retain the spike of rice opening grain husk 1/3 to 2/3, reject the grain husk that grain husk is spent and base portion is the excessively tender flower opened, the grain husk that can bloom in 2-3 days selected and remain is spent and is directly cut grain husk 1/2, leaves in adapt circumstance by the plant sheared, and fringe head sprays in right amount and shines clear water moisturizing;
In step s 102, pollinated morning on the same day, prepared the warm water of 45 DEG C, a kettle, at front 2 hours that female parent is normally bloomed, immersed by the spike of rice sheared in 45 DEG C of warm water and take out after 3-5 minute, flower pesticide extends successively, flick the flower pesticide of elongation, make column cap exposed, be convenient to pollination;
In step s 103, the female parent after gametocide is put kraft paper bag, move near male parent, when containing flower, get male parent pollination;
In step S104, the female parent after pollination is concentrated and plants together, so that management, make regular check on simultaneously, perform insect protected, mildew-resistant work, cut receipts when crossbreed is ripe.
In embodiments of the present invention, in step S101, the gametocide female parent of selection is japonica rice.
In embodiments of the present invention, in step S101, must the grain husk flower opened be rejected clean before cutting grain husk.
In embodiments of the present invention, in step s 102, after within 3-5 minute, taking out if to be immersed by the spike of rice sheared in 45 DEG C of warm water, flower pesticide does not extend, can again by spike of rice as 5-8 minute in the hot gas bottle of 40 DEG C, stimulate flower pesticide to extend.
In embodiments of the present invention, in step s 102, the temperature of gametocide controls between 43-45 DEG C, and time controling is at 3-5 minute, and simultaneously the visual kind of temperature and time of gametocide and weather condition appropriateness change.
In embodiments of the present invention, the time of gametocide will control 2 hours before hybridization female parent is bloomed, and is convenient to stimulate flower pesticide to extend.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
The object of the invention is to solve problems of the prior art with not enough, advance gametocide technology fast development, the method for the japonica rice lukewarm water gametocide that the embodiment of the present invention provides comprises the following steps:
A) bloom and dig out needing to do the female parent band soil of hybridizing noon before that day, cut off old and excessively tender tillering, retain the spike of rice opening grain husk 1/3 to 2/3, reject the grain husk that grain husk is spent and base portion is the excessively tender flower opened, the grain husk that can bloom in 2-3 days selected and remain is spent and is directly cut grain husk 1/2, leave in adapt circumstance by the plant sheared, fringe head sprays in right amount and shines clear water moisturizing;
B) pollinated morning on the same day, prepare the warm water of 45 DEG C, a kettle, at front 2 hours that female parent is normally bloomed, the spike of rice sheared to be immersed in 45 DEG C of warm water after 3-5 minute and takes out, flower pesticide extends successively (if flower pesticide does not extend, can again by spike of rice as 5-8 minute in the hot gas bottle of 40 DEG C, flower pesticide is stimulated to extend), flick the flower pesticide of elongation, make column cap exposed, be convenient to pollination;
C) female parent after gametocide is put kraft paper bag, move near male parent, when containing flower, get male parent pollination;
D) plant concentrated for the female parent after pollination together, so that management, make regular check on simultaneously, perform insect protected, mildew-resistant work, cut receipts when crossbreed is ripe.
Below for Dao88Ji town, town rice No. 14, the specific embodiment step of the method for this japonica rice lukewarm water gametocide of simple declaration:
Town rice 88 is an anti-bar, precocious japonica rice varieties that Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute is bred as in early days.
Town rice No. 14 is that product are of fine quality, output is high, the japonica rice variety of better resistance for of being newly bred as in Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute.
Cross method:
A) select that there is high-quality, anti-stripe virus disease, yielding ability be good, colony's Self-regulation ability strong, Japonica rice town rice No. 14 for hybridization maternal, good, ripe mutually good, the strong root of selected plant Leaf pattern strengthens that stalk, bud gesture are strong, town rice 88 makes male parent.
B) afternoon August 26, dug out by town rice No. 14 even soil, point evil of pruning always and not yet earing, selects and remain and opens the spike of rice of clever 1/3-2/3, and prune to have opened and spend with excessively tender Ying, remaining Ying spends and directly cuts 1/2 Ying's shell, treats gametocide process in second day.
C) start the morning 9: 30 August 27, the fringe head of the hybridization maternal town rice No. 14 of cutting Ying is poured in the warm-water bottle of 45 DEG C 3-5 minute take out, flower pesticide extends successively, flick flower pesticide, the fringe head do not extended by flower pesticide after half an hour inserts in the hot gas bottle taking-up in 5-8 minute of about 40 degree, if do not extend flower pesticide in addition, then directly wipe out.
D) the maternal town rice No. 14 after gametocide is put kraft paper bag, move near male parent town rice 88, when town rice 88 contains flower, get fringe pollination.
E) plant concentrated for the hybridization female parent after pollination together, so that management, make regular check on simultaneously, regularly spray agricultural chemicals, perform insect protected, mildew-resistant work, 9/18, cut fringe sowing.
Above step is summarized as: August 26, lower westerly zone soil dug out town rice 461, and cut clever shell 1/2; Morning August 27,45 DEG C of warm water gametocide 3-5 minute, flew the flower pesticide bullet of elongation, bagging, and town rice 88 is male parent pollination; September 19 sowing.
The present invention has following beneficial effect:
1. break through the restriction of japonica rice hybridization time, improve hybridization efficiency
Conventional lukewarm water gametocide cut grain husk the same day in pollination, and the method after improvement can cut grain husk in noon before that day and even evening, substantially prolongs and cuts the grain husk time, and the hybridization quantity that people does can double above.
2. Pollination Effect is good, and outcrossing seed-setting rate improves
Common lukewarm water gametocide is in pollination gametocide in morning on the same day, and because distance is maternal, natural to open the time spent also long, the more difficult elongation of most of japonica rice flower pesticide, and during pollination, flower pesticide blocking column cap, affects male parent and pollinate; And method after improvement female parent normally natural bloom before use warm water gametocide in 2 hours, the elongation of maternal flower pesticide can be stimulated rapidly, as long as flick flower pesticide, column cap just can be made exposed, greatly improve the outcrossing seed-setting rate that male parent is pollinated.
3. compare with glume-cutting, anther-cutting, male-killing method, to cutting, clever technical requirement is low
Glume-cutting, anther-cutting, male-killing method, should break flower pesticide, can not damage column cap again, and whether the lukewarm water gametocide of improvement only needs to cut clever shell, break do not require flower pesticide.
4. compare with lukewarm water gametocide, glume-cutting, anther-cutting, male-killing method, gametocide is more thorough.
After warm water process, most flower pesticide is killed, even if a small amount of pollen has vigor, flower pesticide also easily ejects clever shell after extending, and compare with lukewarm water gametocide, glume-cutting, anther-cutting, male-killing method, pseudostationary is considerably less.
The method of the japonica rice lukewarm water gametocide that the embodiment of the present invention provides, one afternoon needs are done the female parent of hybridizing before flowering to dig out, cut off old and excessively tender tillering, and rejected the grain husk flower opened and the excessively tender grain husk flower of base portion, the grain husk that can bloom in 2-3 days selected and remain is spent and is directly cut clever 1/2; Pollinating morning on the same day, prepare the warm water of 45 DEG C, a kettle, at front 2 hours that female parent is normally bloomed, immersed by the spike of rice sheared in 45 DEG C of warm water and take out after 3-5 minute, flower pesticide extends successively, flicks the flower pesticide of elongation, makes column cap outside exposed, is convenient to pollination; Female parent after gametocide is put kraft paper bag, moves near male parent, when containing flower, get male parent pollination; Female parent after pollination is concentrated and plants together, so that management, make regular check on simultaneously, perform insect protected, mildew-resistant work, cut receipts when crossbreed is ripe; The method of this japonica rice lukewarm water gametocide breaches the restriction of japonica rice hybridization time, improve hybridization efficiency, Pollination Effect is good, outcrossing seed-setting rate improves, compare with glume-cutting, anther-cutting, male-killing method, to cutting, clever technical requirement is low, compares with lukewarm water gametocide, glume-cutting, anther-cutting, male-killing method, gametocide is more thorough, has stronger propagation and employment and is worth.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (1)
1. a method for japonica rice lukewarm water gametocide, is characterized in that, the method comprises the following steps:
Step one, bloom and dig out needing to do the female parent band soil of hybridizing noon before that day, cut off old and excessively tender tillering, retain the spike of rice opening grain husk 1/3 to 2/3, reject the grain husk that grain husk is spent and base portion is the excessively tender flower opened, the grain husk that can bloom in 2-3 days selected and remain is spent and is directly cut grain husk 1/2, and leave in adapt circumstance by the plant sheared, fringe head sprays clear water moisturizing in right amount;
Step 2, pollinated morning on the same day, prepared the warm water of 45 DEG C, a kettle, and at front 2 hours that female parent is normally bloomed, immersed by the spike of rice sheared in 45 DEG C of warm water and take out after 3-5 minute, flower pesticide extends successively, flicks the flower pesticide of elongation, makes column cap exposed, is convenient to pollination;
Step 3, puts kraft paper bag by the female parent after gametocide, moves near male parent, gets male parent pollination when containing flower;
Step 4, concentrates the female parent after pollination and plants together, so that management, make regular check on simultaneously, perform insect protected, mildew-resistant work, cuts receipts when crossbreed is ripe;
In step one, the gametocide female parent of selection is japonica rice;
In step one, must the grain husk flower opened be rejected clean before cutting grain husk;
In step 2, after within 45 DEG C of warm water 3-5 minute, taking out if immersed by the spike of rice sheared, flower pesticide does not extend, then spike of rice is placed in the hot gas bottle 5-8 minute of 40 DEG C, stimulates flower pesticide to extend;
In step 2, the temperature of gametocide controls at 45 DEG C, and time controling is at 3-5 minute, and the temperature and time of gametocide looks kind and the change of weather condition appropriateness simultaneously;
The time of gametocide will control 2 hours before hybridization female parent is bloomed, and is convenient to stimulate flower pesticide to extend.
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| CN103858748A (en) * | 2012-12-12 | 2014-06-18 | 东港市农业技术推广中心 | Breeding method of new rice variety |
| CN104885928A (en) * | 2015-06-14 | 2015-09-09 | 安徽徽大农业有限公司 | Rice hybridization and castration method |
| CN105123496A (en) * | 2015-07-17 | 2015-12-09 | 浙江省农业科学院 | Paddy rice hybridization method |
| CN106171945A (en) * | 2016-07-11 | 2016-12-07 | 合肥扬扬农业科技有限公司 | A kind of castration method for rice hyberdization |
| CN107360965A (en) * | 2017-08-28 | 2017-11-21 | 贵州省农作物品种资源研究所 | A kind of quick test cross compound formulation of rice |
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