CN1799334A - Glume-cutting, anther-cutting, male-killing paddy rice cross breeding method - Google Patents
Glume-cutting, anther-cutting, male-killing paddy rice cross breeding method Download PDFInfo
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- CN1799334A CN1799334A CN 200510137105 CN200510137105A CN1799334A CN 1799334 A CN1799334 A CN 1799334A CN 200510137105 CN200510137105 CN 200510137105 CN 200510137105 A CN200510137105 A CN 200510137105A CN 1799334 A CN1799334 A CN 1799334A
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Abstract
The invention discloses a method of shearing glume and flower pesticide to kill masculinity for rice hybrid, with detail steps below: carrying out sexual hybridization with rice of same species, shearing 1/4-2/5 of the glume of not blooming in the hybrid female parent from the afternoon of last day to today, which shears off the flower pesticide but does not damage the chapiter, spraying clean water immediately to reach effect of killing masculinity; vibrating the rice treated with above steps before pollination to remove the flower pesticide from the chaff, then covering bags, pollinating the pollen of male parent into the glume, and getting the sexual hybridized seed of rice. The invention employs method of shearing plume and flower pesticide to kill masculinity for mass sexual hybridization of rice, which increases the hybridization efficiency, the plume and flower pesticide shearing rate and masculinity killing rate is greater than 98%, and is also characterized by the convenient, fast, and good effect, when comparing with normal sexual hybridization it can get more hybrid seeds and meet the demand of mass hybridization combination, which increases the hybridization efficiency greatly, homologous to the efficiency and effect of employing rice of male sterility line for hybridization.
Description
Technical field
The present invention relates to paddy rice sexual hybridization method category, relate in particular to a kind of paddy rice cross breeding method of glume-cutting, anther-cutting, male-killing.
Background technology
China produces grain big country, paddy rice is a first crop in the grain, in the rice modification breeding, the basic skills of new rice variety seed selection is crossbreeding, in this process, the rice varieties sexual hybridization, it is that employing traditional " lukewarm water " is killed hero and cut clever artificial emasculation that its female parent kills magnificent major part, both inconvenient, wastes time and energy again, sexual hybridization efficient is low, especially in biotechnology breeding research, need a large amount of combos, existing sexual hybridization method is difficult to accomplish, make up a large amount of near isogene introgressive lines such as " global paddy rice molecular breeding ", adopt 118 of donor parents, 2 of donor parents, each donor and donor parent are hybridized and are backcrossed, each was backcrossed and will do 5000 of hybridization quantity from generation to generation in a season, and adopting conventional sexual hybridization method is to accomplish.Tracing it to its cause, is that sexual hybridization efficient is low because of time-consuming taking a lot of work in the artificial emasculation process.
Summary of the invention
The objective of the invention is to: overcome the deficiencies in the prior art, propose a kind of paddy rice cross breeding method of glume-cutting, anther-cutting, male-killing, be same as with the efficient that improves the paddy rice sexual hybridization and utilize male sterible series of rice to carry out hybridization efficiency and effect.
The present invention implements by the following technical solutions: with sexual hybridization between the rice varieties, to hybridize maternal clever flower shears of not blooming before blooming noon before that day to the same day and remove 1/4-3/5, both break flower pesticide, not damage column cap again, and spray clear water immediately, reach gametocidal effect; Before the pollination, the spike of rice behind the glume-cutting, anther-cutting, male-killing is trembled bullet, the flower pesticide during grain husk is spent ejects outside the clever shell, and bagging is then invested paternal pollen in the grain husk of cutting behind the grain husk spends, and obtains paddy rice sexual hybridization seed at last.
Clever flower shears removes 1/4-3/5 in the inventive method, both can wipe out the most of flower pesticide that is stretched to the top, can break the flower pesticide that does not extend the middle and lower part again.Break flower pesticide its interior pollen grain is exposed in the air, can not shed behind the spray clear water outside the flower pesticide, reach and kill male purpose.The grain husk flower shears goes 1/2 for best.
The present invention adopts the glume-cutting, anther-cutting, male-killing method, can carry out the sexual hybridization of paddy rice in a large number, improve the efficient of sexual hybridization, the glume-cutting, anther-cutting, male-killing rate can reach more than 98%, and is convenient and swift, effective, can obtain a large amount of crossbreed with conventional sexual hybridization than more, also can satisfy simultaneously the needs of a large amount of hybridization combos, improve sexual hybridization efficient greatly, be equivalent to efficient and the effect of utilizing male sterible series of rice to do hybridization.
Embodiment
The present invention is described in detail below in conjunction with embodiment.
Embodiment 1:
Present embodiment is with different rice varieties sexual hybridizations, to rice varieties " Gumei2 " as female parent, " M3122 ", " IR72 " cuts off the clever shell of 1/4-3/5 noon before that day in hybridization, accomplish to break or wipe out flower pesticide, and spray clear water immediately, stop pollen to shed, before blooming in second day, right-handed mother refers to make a concerted effort to play tassel several times with forefinger, remain in the flower pesticide of grain husk in spending behind the grain husk and eject cutting, bagging then, award on the female parent of glume-cutting, anther-cutting, male-killing with male parent " 752 " pollen when blooming, just can obtain hybrid seed after 20-25 days, the hybrid seed sowing on hybrid strain (female parent and male parent) next door, is identified true and false hybrid, and the paddy rice cross breeding method of glume-cutting, anther-cutting, male-killing obtains true crossbreed average out to 98.4% as a result.
Embodiment 2:
Before hybridizing the same day, blooming, cut off clever shell 1/2 as the rice varieties " I R58025 " of hybridizing female parent, " purple extensive 100 ", " special blue or green ", to break flower pesticide or to wipe out the flower pesticide that extends the top, and the even clear water of spray immediately, stop pollen to shed, bagging then, maternal self-fruitful rate after checking glume-cutting, anther-cutting, male-killing after 20 days, average out to 0.2% as a result.
Claims (4)
1, a kind of paddy rice cross breeding method of glume-cutting, anther-cutting, male-killing is characterized in that: during the paddy rice sexual hybridization, will hybridize maternal clever flower shears of not blooming before blooming noon before that day to the same day and remove 1/4-3/5, both broken flower pesticide, do not damage column cap again, and spray clear water immediately, reach gametocidal effect; Before the pollination, the spike of rice behind the glume-cutting, anther-cutting, male-killing is trembled bullet, the flower pesticide during grain husk is spent ejects outside the clever shell, and bagging is pollinated with the hybridization male parent then.
2, according to the paddy rice cross breeding method of claims 1 described glume-cutting, anther-cutting, male-killing, it is characterized in that: clever flower shears removes 1/4-3/5, both can wipe out the most of flower pesticide that is stretched to the top, can break the flower pesticide that does not extend the middle and lower part again.
3, according to the paddy rice cross breeding method of claims 1 or 2 described glume-cutting, anther-cutting, male-killings, it is characterized in that: break flower pesticide its interior pollen grain is exposed in the air, can not shed behind the spray clear water outside the flower pesticide, reach and kill male purpose.
4, according to the paddy rice cross breeding method of claims 1 or 2 described glume-cutting, anther-cutting, male-killings, it is characterized in that: clever flower shears goes 1/2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510137105 CN1799334A (en) | 2005-12-29 | 2005-12-29 | Glume-cutting, anther-cutting, male-killing paddy rice cross breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510137105 CN1799334A (en) | 2005-12-29 | 2005-12-29 | Glume-cutting, anther-cutting, male-killing paddy rice cross breeding method |
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| CN1799334A true CN1799334A (en) | 2006-07-12 |
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| CN 200510137105 Pending CN1799334A (en) | 2005-12-29 | 2005-12-29 | Glume-cutting, anther-cutting, male-killing paddy rice cross breeding method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907314A (en) * | 2012-11-09 | 2013-02-06 | 韦炳辉 | Selection and breeding method for early rice variety |
| CN103430833A (en) * | 2013-08-23 | 2013-12-11 | 江西省农业科学院水稻研究所 | Novel male-killing method for sexual hybridization of rice |
| CN107711491A (en) * | 2017-11-02 | 2018-02-23 | 郴州市农业科学研究所 | A kind of method of rice sexual hybridization |
| CN109169264A (en) * | 2018-10-25 | 2019-01-11 | 江西省超级水稻研究发展中心 | A kind of paddy rice cross breeding pollinating method |
| CN109287352A (en) * | 2018-09-04 | 2019-02-01 | 山东省林业科学研究院 | A method of improving elder artificialpollination percentage of fertile fruit |
| CN111657137A (en) * | 2019-03-07 | 2020-09-15 | 内蒙古农业大学 | Oat hybridization method |
| CN116439124A (en) * | 2023-03-29 | 2023-07-18 | 永州市农业科学研究所 | Water-immersed glume Quan Ying emasculation method and application thereof in rice crossbreeding |
-
2005
- 2005-12-29 CN CN 200510137105 patent/CN1799334A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907314A (en) * | 2012-11-09 | 2013-02-06 | 韦炳辉 | Selection and breeding method for early rice variety |
| CN103430833A (en) * | 2013-08-23 | 2013-12-11 | 江西省农业科学院水稻研究所 | Novel male-killing method for sexual hybridization of rice |
| CN103430833B (en) * | 2013-08-23 | 2015-07-29 | 江西省农业科学院水稻研究所 | A kind of paddy rice sexual hybridization gametocide new method |
| CN107711491A (en) * | 2017-11-02 | 2018-02-23 | 郴州市农业科学研究所 | A kind of method of rice sexual hybridization |
| CN109287352A (en) * | 2018-09-04 | 2019-02-01 | 山东省林业科学研究院 | A method of improving elder artificialpollination percentage of fertile fruit |
| CN109169264A (en) * | 2018-10-25 | 2019-01-11 | 江西省超级水稻研究发展中心 | A kind of paddy rice cross breeding pollinating method |
| CN111657137A (en) * | 2019-03-07 | 2020-09-15 | 内蒙古农业大学 | Oat hybridization method |
| CN116439124A (en) * | 2023-03-29 | 2023-07-18 | 永州市农业科学研究所 | Water-immersed glume Quan Ying emasculation method and application thereof in rice crossbreeding |
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