CN106939328A - A kind of method that the tawny daylily germplasm of Resistant Gene To Rust germ is cultivated in quick screening - Google Patents
A kind of method that the tawny daylily germplasm of Resistant Gene To Rust germ is cultivated in quick screening Download PDFInfo
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- CN106939328A CN106939328A CN201710118582.7A CN201710118582A CN106939328A CN 106939328 A CN106939328 A CN 106939328A CN 201710118582 A CN201710118582 A CN 201710118582A CN 106939328 A CN106939328 A CN 106939328A
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Abstract
A kind of method that the tawny daylily germplasm of Resistant Gene To Rust germ is cultivated the invention discloses quick screening.Tawny daylily parent refrigerates 30 60 days under the conditions of 48 DEG C immediately after hybridizing the tawny daylily seed collection of gained then；Then sow, planting environment is the greenhouse of artificial regulating and controlling temperature humidity, temperature is 15 30 DEG C；The tawny daylily hybrid seed long sprinkling for starting the 1st Spores suspension to the cm of seedling 10 after germination, Spores spray concentration is controlled 106‑107Plastic sheeting moisturizing is covered after individual/ml, sprinkling 3 days, then remove plastic sheeting, sprinkling in every 30 days once, is sprayed 3 times altogether, is every time wiped out all cm of seedling apical 0.5 1 with secateurs before sprinkling, is manufactured wound, so that Spores are invaded.Whole process of the present invention uses manual intervention, and directive breeding is with clearly defined objective, workable, is easy to implement, as a result reliable and stable.
Description
Technical field
A kind of method that the tawny daylily germplasm of Resistant Gene To Rust germ is cultivated the present invention relates to quick screening, belongs to ornamental plant breeding
Field.
Background technology
Tawny daylily is world-renownedly by class Perennial Flowers.Its spend greatly, color is gorgeous, the overall florescence is long, because it is widely applied,
More than 90,000 kind has been cultivated in the whole world, but most all in European and American developed countries, and mondial tawny daylily provides
All there is serious rust sensitiveness in source, in the season of growth, plant leaf easily rubiginose germ, cause yellow leaf, life
It is long sluggish, have a strong impact on landscape effect.
Tawny daylily breeding of new variety commercially expands numerous mainly by plant division mainly by hybridization.Due to lacking excellent Resistant Gene To Rust parent,
The improved seeds of current all rare high Resistant Gene To Rust both at home and abroad.
The content of the invention
The purpose of the present invention be for the problem of breeder lacks the tawny daylily germplasm of high Resistant Gene To Rust both at home and abroad at present there is provided
A kind of method that the tawny daylily germplasm of Resistant Gene To Rust germ is cultivated in quick screening.
Technical scheme is as follows：
A kind of method that the tawny daylily germplasm of Resistant Gene To Rust germ is cultivated in quick screening, is comprised the following steps that：
Tawny daylily parent refrigerates 30-60 days under the conditions of 4-8 DEG C immediately after hybridizing the tawny daylily seed collection of gained then；Then broadcast
Kind, planting environment is the greenhouse of artificial regulating and controlling temperature humidity, and temperature is 15-30 DEG C；Tawny daylily hybrid seed is grown to children after germination
The cm of seedling 10 starts the sprinkling of the 1st Spores suspension, and Spores spray concentration is controlled 106-107Covered after individual/ml, sprinkling
Plastic sheeting moisturizing 3 days, then removes plastic sheeting, and sprinkling in every 30 days once, is sprayed 3 times altogether, every time will with secateurs before sprinkling
All seedling apical 0.5-1 cm are wiped out, and manufacture wound, so that Spores are invaded.Resistant Gene To Rust seedling disease is not serious very
To death, disease-resistant growth of seedling is normal, and to month in next year 4-5, most kinds can bloom, and accelerate breeding process, obtain
Resistant gerplasm.
The Spores are fresh to be taken from susceptible tawny daylily maternal plant, plants in the greenhouse of artificial regulatory temperature humidity, temperature
For 15-30 DEG C；Every time before sprinkling, fresh pathogen is cut from cause of disease maternal plant to be dissolved in after 121 DEG C of 25-30min autoclavings and steamed
In distilled water, microscope differentiates and counted, and spray concentration is controlled 106-107Individual/ml.
The seedling cultivation is in the cm of bore 15, deep 12 cm plastic tub, until blooming.
Using above technical scheme, compared with prior art, with advantages below：
1）Whole process of the present invention uses manual intervention, and directive breeding is with clearly defined objective, workable, is easy to implement, and as a result stablizing can
Lean on.The seed selection of traditional tawny daylily Resistant gerplasm is all to use crop field seed selection, and pathogeny first is unstable, can only be caught an illness by environment；Its
Secondary evaluation method not science；3rd, susceptible, disease resistance trait is affected by environment larger；4th, the traditional breeding method cycle is long, mesh
Mark is indefinite；5th, even if selecting Resistant variants, it is also necessary to long-term observation, or even artificial infection Spores are evaluated
Its disease resistance.
2）The present invention uses Seeds preprocess technology, and deepfreeze 30-60 days drastically increases seed germination rate and gone out
Seedling regularity.
Traditional tawny daylily breeding, is all that seminal propagation is substantially all using vegetative propagations such as plant division, tissue cultures in addition to breeding
Be adopt broadcast or adopt after refrigerate, sow after year.Adopting to broadcast can cause germination very irregular, or even germination percentage is low
Under, bring very big inconvenience to final-period management.Refrigerate and sowed after year, although ensure that and emerge neatly, but postponed breeding significantly
In the cycle, at least half a year growth time is wasted.And the present invention proposes that refrigeration allows for tawny daylily seed maturity for 30-60 days
Phase is inconsistent, about differs 30 days, in order to harvest in batches, concentrates sowing, refrigerates 30-60 days, can meet the after-ripening, low of seed
It is warm to require, and can unified, concentration sowing, raising breeding, raising seedling efficiency.
3）Pathogenic strain used in the present invention, from the maternal disease in the tawny daylily normal plants stage, obtained every time
Opportunistic pathogen is fresh material, pathogenic strong, without numerous and diverse artificial culture, and existing enchashment is used, convenient and reliable, simple and easy to apply.Tradition
The seed selection of tawny daylily Resistant gerplasm, is substantially lived at the mercy of the elements, and natural selection is occasionally found, without engineer and participation.
4）The present invention about the Breeding Process of tawny daylily Resistant gerplasm is carried out in the greenhouse of temperature-controllable, temperature control
At 15-30 DEG C, it is adapted to tawny daylily plant strain growth, winter not dormancy, most kinds can bloom in month in next year 4-5, can greatly contract
Short seed is seeded into the ripe growth cycle bloomed.Traditional tawny daylily crossbreeding, is usually in autumn sowing then or the beginning of spring in next year
Sowing, outdoor plantation, no matter any, being typically necessary can just yield positive results across 3 years, and breeding cycle is very long.
Embodiment
The present invention is further described with reference to specific embodiment, following examples are used to illustrate the present invention, but
It is not used to
Limit the scope of the invention.
Embodiment 1
Our unit has collected tawny daylily cultivars and strains more than 300, and annual early April enters the early flowering season, and late June enters Flowers ending.
Artificial pollination is carried out during this period, 6-8 months, seed is ripe successively, refrigerator is immediately placed in after maturation, it is cold under the conditions of 4-8 DEG C
Hide processing 30-60 days.This is to ensure seed bud rate occurred frequently, the premise for neat and consistent of emerging.
1000 Seed Treatments will be amounted to finish, sowed.Using 128 hole disk sowings, substrate composition is peat：It is precious
Zhu Yan：Vermiculite=10:3:2, per 2, cave seed, water, 20-30 DEG C of progress nursery are poured after broadcasting.About 15-20 days, seedling was basic
All sprout.
10 the end of month then, tawny daylily seedling is moved into wide 15 cm, deep 12 cm plastic tub, substrate composition is, peat：It is precious
Zhu Yan：Commercially available decomposed manure=10:4:3.Routine Management after field planting is good, basin soil see it is dry see wet, temperature control is in 15-30
DEG C, while attention control pest and disease damage.
Then 11 the middle ten days and the last ten days, when tawny daylily seedling length is to more than 10cm, start to carry out germ inoculation to it.Symptom is chosen first
Obvious maternal plant, intersection clip 0.5-1cm is good for sterilizing scissors from disease2The susceptible tissue of size is some, puts into known volume
Sterile purified water in, take back laboratory and analyzed and identified, and calculate bacterial concentration.Bacterial concentration is finally diluted to 106-
107Individual/ml or so, it is standby.
All tawny daylily seedling are carried out to make wound, that is, cut off plant top blade 0.5-1cm, it is then that above-mentioned bacterium solution is uniform
It is sprayed on tawny daylily plant, then sets up shed, plastic covering film keeps humidity and temperature in canopy, removed after 3 days.One week
Different degrees of susceptible symptom, normal management occurs in left and right, most of tawny daylily seedling.
Hereafter bacterium solution sprinkling processing was carried out to tawny daylily seedling every 30 days, method is with the 1st time, coprocessing 3 times.
Next year by the end of February, through statistics, germinates 823 plants altogether, germination percentage 82.3%, wherein there is 11 plants of tawny daylilies to have significantly rust
Resistance, they are in addition to naturally-aged blade, almost without obvious rust symptom.Remaining plant, has 9.5% death, shows as leaf
Piece whole yellows, butt rot, the tawny daylily of residue 89.1% is susceptible serious, and the old and new's blade is all with rust spot, growth retardation,
But root is substantially all healthy.
The present invention carries out Resistant gerplasm choosing by carrying out artificially controlling temperature cultivation to tawny daylily seedling, and spraying rust pathogen
Educate, the tawny daylily seedling that there are about 1.3% through statistics has good Resistant Gene To Rust ability, this is just to be entered from now on using these Resistant variants
Row crossbreeding, cultivating Resistant Gene To Rust tawny daylily, there is provided may.
Claims (3)
1. a kind of method that the tawny daylily germplasm of Resistant Gene To Rust germ is cultivated in quick screening, it is characterised in that：Comprise the following steps that：
Tawny daylily parent refrigerates 30-60 days under the conditions of 4-8 DEG C immediately after hybridizing the tawny daylily seed collection of gained then；Then broadcast
Kind, planting environment is the greenhouse of artificial regulating and controlling temperature humidity, and temperature is 15-30 DEG C；Tawny daylily hybrid seed is grown to children after germination
The cm of seedling 10 starts the sprinkling of the 1st Spores suspension, and Spores spray concentration is controlled 106-107Covered after individual/ml, sprinkling
Plastic sheeting moisturizing 3 days, then removes plastic sheeting, and sprinkling in every 30 days once, is sprayed 3 times altogether, every time will with secateurs before sprinkling
All seedling apical 0.5-1 cm are wiped out, and manufacture wound, so that Spores are invaded.
2. according to the method described in claim 1, it is characterised in that：The Spores are fresh to be taken from susceptible tawny daylily maternal plant,
Plant in the greenhouse of artificial regulatory temperature humidity, temperature is 15-30 DEG C；Every time before sprinkling, from cause of disease maternal plant by fresh pathogen
Cut and be dissolved in after 121 DEG C of 25-30min autoclavings in distilled water, microscope differentiates and counted, spray concentration is controlled 106-
107Individual/ml.
3. according to the method described in claim 1, it is characterised in that：The seedling cultivation is in the cm of bore 15, deep 12 cm modeling
In feed basin, until blooming.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710118582.7A CN106939328A (en) | 2017-03-01 | 2017-03-01 | A kind of method that the tawny daylily germplasm of Resistant Gene To Rust germ is cultivated in quick screening |
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| CN201710118582.7A CN106939328A (en) | 2017-03-01 | 2017-03-01 | A kind of method that the tawny daylily germplasm of Resistant Gene To Rust germ is cultivated in quick screening |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511415A (en) * | 2019-01-18 | 2019-03-26 | 江苏省农业科学院宿迁农科所 | A kind of inoculation cultural method of day lily rust |
| CN112056199A (en) * | 2020-09-14 | 2020-12-11 | 浙江省萧山棉麻研究所 | Seedling storage and breeding method for hemerocallis fulva |
Citations (4)
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|---|---|---|---|---|
| CN101911895A (en) * | 2010-07-20 | 2010-12-15 | 云南省农业科学院甘蔗研究所 | Artificial inoculation method for authenticating resistance of sugarcane rust diseases |
| WO2014135682A1 (en) * | 2013-03-08 | 2014-09-12 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing mybtf |
| CN104604516A (en) * | 2015-03-02 | 2015-05-13 | 漯河市农业科学院 | Method for wheat disease resistance breeding |
| CN105557347A (en) * | 2014-10-13 | 2016-05-11 | 江西省农业科学院植物保护研究所 | Gray mold resistance identified seedling stage inoculation method for capsicum |
-
2017
- 2017-03-01 CN CN201710118582.7A patent/CN106939328A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101911895A (en) * | 2010-07-20 | 2010-12-15 | 云南省农业科学院甘蔗研究所 | Artificial inoculation method for authenticating resistance of sugarcane rust diseases |
| WO2014135682A1 (en) * | 2013-03-08 | 2014-09-12 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing mybtf |
| CN105557347A (en) * | 2014-10-13 | 2016-05-11 | 江西省农业科学院植物保护研究所 | Gray mold resistance identified seedling stage inoculation method for capsicum |
| CN104604516A (en) * | 2015-03-02 | 2015-05-13 | 漯河市农业科学院 | Method for wheat disease resistance breeding |
Non-Patent Citations (2)
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| 何淑玲: "《高寒药用植物栽培学》", 30 June 2016, 甘肃科学技术出版社 * |
| 杰夫·霍奇: "《英国皇家园艺学会植物学指南》", 31 May 2016, 重庆大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511415A (en) * | 2019-01-18 | 2019-03-26 | 江苏省农业科学院宿迁农科所 | A kind of inoculation cultural method of day lily rust |
| CN112056199A (en) * | 2020-09-14 | 2020-12-11 | 浙江省萧山棉麻研究所 | Seedling storage and breeding method for hemerocallis fulva |
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Application publication date: 20170711 |