CN100394843C - Long-spike neck sterile line selection and breeding method - Google Patents
Long-spike neck sterile line selection and breeding method Download PDFInfo
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- CN100394843C CN100394843C CNB2006100119548A CN200610011954A CN100394843C CN 100394843 C CN100394843 C CN 100394843C CN B2006100119548 A CNB2006100119548 A CN B2006100119548A CN 200610011954 A CN200610011954 A CN 200610011954A CN 100394843 C CN100394843 C CN 100394843C
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Abstract
The present invention belongs to the field of the utilization of the hybrid vigor of paddy rice. The present invention is the breeding technique that a long spike neck gene eui of recessive high straw paddy rice 02428h is led into a two line sterile line to obtain a long spike neck two line sterile line in a backcross mode by the pathway of genetic improvement. The straw of the sterile line is extracted completely. The length of the straw neck is equal to that of the straw neck of the sterile line which does not contain the eui gene and applies 450 to 600 g of '920' per hechare. The outcross characteristic of the sterile line is improved essentiality on inheritance. The present invention enhances the yield of breeding and producing seeds, reduces production cost and reduces environmental pollution.
Description
Technical field
The present invention relates to the selection of long-spike neck sterile line, belong to the rice heterosis utilization field.
Background technology
Cytoplasmic-nuclear male sterility and light temperature sensing male nuclear are sterile to be the important genetic tool that hybrid rice successfully utilizes.Yet, because sterility and fringe neck degree of stretching out are closely related, thereby no matter be three to be sterile line or two-line sterile line, often be accompanied by serious bag neck phenomenon (be fringe base portion grain husk spend be coated with in Flag Leaf Sheath), have a strong impact on breeding and the hybrid seed yield of sterile line.For this reason, people have invented and have sprayed gibberellin (GA
3, ProductName: method 920) and application technique, though solved the bag neck problem of male sterile line, not only increase breeding cost, also can cause environmental pollution.
1981, Rutger and Carnahan were at the F of japonica rice " Mg/Terso "
3Found in the colony that is gone up an internode elongation type recessive tallness mutant 76:4512 most, be eui (elongated uppermostinternode) with the unnamed gene of this proterties of control and it is produced the 4th hereditary key element after relaying male sterile line, maintenance line and the recovery system as the series of three-series hybrid rice seed.After this, people have successively found a plurality of long fringe neck recessive tall height mutants such as Grlc, Mh-1, " 02428h ", eB-1, eB-2, Gh-2 and Sh-3 again, and have carried out heredity and breeding utilization research.Shen Zongtan etc., Virmani etal, Liang Kang Jing etc. adopt the backcross transformation method with eui gene (Rutger J N, Carnahan H L.A forth geneticelement to facilitate hybrid cereal production-a recessive tall in rice.Crop Sci, 1981,21:373～376) import male sterile line, overcome the bag neck phenomenon of male sterile line, but because the burdensome influence of the heredity that separation reorganization and unfavorable gene linkage bring, the effect of removing the bag neck is often not ideal enough, thereby fails so far to obtain utilizing on producing.The e-hybrid rice breeding technology of the propositions such as Cui Yang Ren is directly to obtain the breeding approach that three of long fringe neck is maintainer, restorer and dual-purpose sterile line by radioinduction.
" 02428h " (Li H-B (Li Hebiao), Sun L-H (Sun Lihua), Zou J-S (Zou Jiangshi), et al.Identification and study of recessive tall stalk and wide compatibility rice germplasm 02428h.Jiangsu J.of Agr.Sci. (Jiangsu agricultural journal), 1992,8 (3): 48～50), it is the recessive tallness mutant that Lee and mark and Sun Lihua etc. are found in the Somatic Cell Culture offspring of wide affine paddy rice " 02428 ", because 02428h has kept 02428 wide affinity characteristic, thereby has special value in the research of subspecies indica and japonica hybrid use of advantage.But its breeding utilization is not reported so far.
Training short 64S (public) and introduce from National Engineering and Technological Research Center of Interbreed Paddy, is a low temperature sensitive sterile strain that comprehensive proterties is good.A series of combinations such as " two line system ", " two excellent E32 ", " two is excellent special blue or green " have been bred with this male sterile line combo.Particularly the pioneer who is described as super hybridized rice that breeds of two line system makes up, and the plant type of " two excellent E32 " is regarded as the ideotype of super hybridized rice.Yet we find that in the production of hybrid seeds production practices fringe degree of stretching out of Peiai 64S is relatively poor, if without GA
3Process, have approximately 50% clever perianth to contain in Flag Leaf Sheath, have a strong impact on outcrossing.Therefore, during with this sterile lines seed making, must use GA
3Processing improves outcrossing seed-setting rate to improve its fringe degree of stretching out.Yet, because this male sterile line is to GA
3Susceptibility relatively poor, GA
3Consumption must be at 450g/hm
2Above its fringe degree of stretching out that just can make is effectively improved.Like this, not only greatly increase breeding cost, reduce seed quality, also can cause environmental pollution.
Summary of the invention
Technical problem
The objective of the invention is in the production of hybrid seeds production practices training short 64S fringe degree of stretching out relatively poor, to GA
3The relatively poor problem of susceptibility, by the genetic improvement approach, with the method for backcrossing the eui gene importing two-line sterile line of 02428h is trained among the short 64S, obtain long fringe neck two-line sterile line P8hS, the fringe degree of stretching out of the short 64S of training is thoroughly improved, and main economic characters and the sterile degree of training short 64S do not had a negative impact.
Technical scheme
Content of the present invention is the selection of long-spike neck sterile line, it is characterized in that, the selection of long-spike neck sterile line comprises:
1) take low-temperature-sensitive two-line sterile line Peiai 64S as maternal, 02428h obtains F for the combination of male parent preparing hybrid
1(Peiai 64S/02428h), and take Peiai 64S as female parent and F
1Backcross and obtain B
1F
1(Peiai 64S//Peiai 64S/02428h);
2) plantation B
1F
1(Peiai 64S//Peiai 64S/02428h), the leaf attitude of selected plant is with Peiai 64S, plant height 75～85cm, the straight and upright curls inward of blade when namely earing, pollen fertility is made male parent at the individual plant more than 90% and is continued to backcross with Peiai 64S, obtains to backcross seed B
2F
1With corresponding male parent B
1F
1Selfed seed B
1F
2, gather in the crops in pairs;
3) plant in pairs B
1F
2With B
2F
1If, at B
1F
2Isolate in the strain neck length of spike more than 10cm, plant height is the recessive tallness plant of 105～115cm, then at its corresponding B
2F
1Select plant height 80～95cm, the straight and upright curls inward of blade, pollen fertility to make male parent at the individual plant more than 90% in the strain and continue to backcross with Peiai 64S, obtain to backcross seed B
3F
1Selfed seed B with corresponding male parent
2F
2, gather in the crops in pairs;
4) plant in pairs B
2F
2With B
3F
1If, at B
2F
2Isolate in the strain neck length of spike more than 10cm, plant height is the recessive tallness plant of 105cm～115cm, then at its corresponding B
3F
1Select plant height 80～95cm, the straight and upright curls inward of blade, spike length 24～26cm in the strain, 190～210 of every fringes, pollen sterility degree cut stubble regeneration breeding at the sterile individual plant more than 99%, obtain B
3F
2Seed;
5) plant above-mentioned B
3F
2Single-strain seed, each individual plant kind becomes a strain, allows its selfing separate, and selects neck length of spike in separating strain more than 10cm, plant height 105～115cm, the straight and upright curls inward of blade, spike length 24～26cm, the individual plant that the every fringe of every fringe is 190～210 allows its selfing obtain B
3F
3Seed;
6) plant above-mentioned B
3F
3Strain, at performance recessive tallness, neck length of spike more than 10cm, plant height 105～115cm, strain in select sterile degree to reach 100% individual plant, cut stubble regeneration breeding and obtain a B
3F
4Seed;
7) plantation B
3F
4Strain, select the neck length of spike degree more than 10cm, the straight and upright curls inward of blade, spike length 24～26cm, the strain that every fringe is 190～210 and (namely 9311) (available from the Lixiahe region in Jiangsu institute of agricultural sciences) the test cross combo of raising rice No. 6, and gather in the crops its selfed seed, acquisition B
3F
5Strain;
8) plantation B
3F
5Strain all shows recessive tallness and pollen sterility, selects wherein that sterile degree reaches 100%, plant height 105～115cm, and the straight and upright curls inward of blade, spike length 24～26cm, the strain that every fringe is 190～210 is the long-spike neck sterile line of acquisition, " P8hS " temporarily by name.
Beneficial effect advantage of the present invention and effect be,
The present invention arrives two-line sterile line with the method for backcrossing with the eui gene transformation of " 02428h " by the genetic improvement approach, obtains long fringe neck two-line sterile line.Take photo-thermo-sensitive genic male sterile line as maternal, recessive tall height mutant 02428h is that male parent is hybridized, and take photo-thermo-sensitive genic male sterile line as the recurrent parent continuous backcross 3 times.The method of taking limit selfing isolation identification, limit to backcross in the backcross process to quicken process from generation to generation, improves breeding efficiency.Leave and take selfed seed the plant that backcrosses at every turn, in next generation, every self progeny takes place to separate at the recessive tallness gene, continues roguing and backcross in its corresponding backcross progeny, and so continuous backcross is to B
3F
1The time, the roguing selfing has obtained to carry the long fringe neck two-line sterile line of eui gene through separation and purification.
With the above-mentioned method of backcrossing two-line sterile line is arrived in the eui gene transformation of " 02428h ", the long fringe neck two-line sterile line fringe that obtains is extracted out fully, the neck length of spike degree is more than 10cm, the effect of using 450-600 gram " 920 " with the male sterile line per hectare that does not contain the eui gene is suitable, can obviously improve breeding, the hybrid seed yield (per hectare improves 2250～3000 kilograms) of male sterile line, reduce production costs, reduce environmental pollution.
Description of drawings
Fig. 1 Pghs (left side) compares with fringe neck degree of stretching out (b) with the strain shape (a) of the short 64S of training (right side)
Embodiment
It is female parent that positive season in 1999 was trained short 64S in Nanjing with low temperature sensitive two-line sterile line, and 02428h is the combination of male parent preparing hybrid.Plant F in Hainan winter then
1(Peiai 64S/02428h), and take Peiai 64S as female parent and F
1Backcross and obtain B
1F
1(Peiai 64S//Peiai 64S/02428h).Positive season plantation B in 2000
1F
1(Peiai 64S//Peiai 64S/02428h), the leaf attitude of selected plant strain is with Peiai 64S, plant height 75～85cm, the straight and upright curls inward of blade when namely earing, pollen fertility is made male parent 13 of the individual plants more than 90% and is continued to backcross with Peiai 64S, and seed B backcrosses after the maturation
2F
1With corresponding male parent B
1F
1Selfed seed gather in the crops in pairs.Planted in pairs B in winter in 2000 in Hainan
1F
2With B
2F
1At 13 B
1F
2Have in the strain 9 strains isolate neck length of spike more than 10cm, plant height is the recessive tallness plant of 105～115cm.At its corresponding 8 B
2F
1Selecting plant height 80～95cm, the straight and upright curls inward of blade, pollen fertility to make male parent at 30 individual plants more than 90% in the strain continues to backcross with Peiai 64S.Seed B backcrosses after the maturation
3F
1Gather in the crops in pairs with the selfed seed of corresponding male parent.Calendar year 2001 in positive season is at 30 couples of B of Nanjing plantation
2F
2With B
3F
1, the result has 18 B
2F
2Strain is isolated the recessive tallness plant.At corresponding 16 B
3F
1Select plant height 80～95cm, the straight and upright curls inward of blade, spike length 24～26cm in the strain, 190～210 of every fringes, pollen sterility degree reach 114 of sterile individual plants 99% or more, cut the stubble breeding seed of regenerating.Winter calendar year 2001 is at 114 B of Hainan plantation
3F
2Strain allows its selfing separate.In 59 separated strain systems, select neck length of spike more than 10cm, plant height 105～115cm, the straight and upright curls inward of blade, spike length 24～26cm, 190 of the individual plants that the every fringe of every fringe is 190～210.190 B of positive season plantation in 2002
3F
3Strain, 32 at the performance recessive tallness, more than the neck length of spike 10cm, select sterile degree to reach 62 of 100% individual plants in the strain of plant height 105～115cm, cut a stubble regeneration breeding seed.Winter in 2002 is at 62 B of Hainan plantation
3F
4Strain, 15 neck length of spike degree more than 10cm, the straight and upright curls inward of blade, about spike length 24～26cm, select economical character stable and consistent, plant height 105～115cm, the straight and upright curls inward of blade in the strain that every fringe is 190～210, spike length 24～26cm, 46 of 190～210 individual plants of every fringe and 9311 test cross combos, and gather in the crops its selfed seed, obtain B
3F
5Strain; 46 B of positive season plantation in 2003
3F
5Strain all shows recessive tallness and pollen sterility, and wherein 5 sterility and economical character are stable, and sterile degree reaches 100%, plant height 105～115cm, the straight and upright curls inward of blade, spike length 24～26cm, the strain that every fringe is 190～210 is the long-spike neck sterile line of acquisition, " P8hS " temporarily by name.
The fringe of the two-line sterile line " P8hS " that obtains by above method is extracted out fully, and the neck length of spike degree is more than 10cm
(Fig. 1), training short 64S per hectare with the two-line sterile line that does not contain the eui gene, to use the effect of 450-600 gram " 920 " suitable, can obviously improve breeding, the hybrid seed yield (per hectare improves 2250～3000 kilograms) of male sterile line, reduce production costs, reduce environmental pollution.
Claims (1)
1. the selection of long-spike neck sterile line is characterized in that, this method comprises the steps:
1) take low-temperature-sensitive two-line sterile line Peiai 64S as maternal, 02428h obtains F for the combination of male parent preparing hybrid
1, and take Peiai 64S as female parent and F
1Backcross and obtain B
1F
1
2) plantation B
1F
1, the leaf attitude of selected plant is with Peiai 64S, and plant height 75～85cm, the straight and upright curls inward of blade and pollen fertility are made male parent at the individual plant more than 90% and are continued to backcross with Peiai 64S when namely earing, and obtain to backcross seed B
2F
1B
2F
1With corresponding male parent B
1F
1Selfed seed B
1F
2, gather in the crops in pairs;
3) plant in pairs B
1F
2With B
2F
1, at B
1F
2Isolate neck length of spike in the strain more than 10cm and plant height is the recessive tallness plant of 105～115cm, then with B
1F
2The corresponding B of strain
2F
1Select plant height 80～95cm, the straight and upright curls inward of blade and pollen fertility to make male parent at the individual plant more than 90% in the strain and continue to backcross with Peiai 64S, obtain to backcross seed B
3F
1B
3F
1Selfed seed B with corresponding male parent
2F
2, gather in the crops in pairs;
4) plant in pairs B
2F
2With B
3F
1, at B
2F
2Isolate neck length of spike in the strain more than 10cm and plant height is the recessive tallness plant of 105cm～115cm, then with B
2F
2The corresponding B of strain
3F
1Select plant height 80～95cm, the straight and upright curls inward of blade, spike length 24～26cm, 190～210 of every fringes and pollen sterility degree at the sterile individual plant more than 99% in the strain, cut stubble regeneration breeding, obtain B
3F
2Seed;
5) plant above-mentioned B
3F
2Single-strain seed, each individual plant kind becomes a strain, allows its selfing separate, in separating strain, select neck length of spike more than 10cm, the individual plant of 190～210 of plant height 105～115cm, the straight and upright curls inward of blade, spike length 24～26cm and every fringes, allow its selfing obtain B
3F
3Seed;
6) plant above-mentioned B
3F
3Strain is showing recessive tallness, is selecting sterile degree to reach 100% individual plant more than the neck length of spike 10cm and in the strain of plant height 105～115cm, cuts stubble regeneration breeding and obtains a B
3F
4Seed;
7) plantation B
3F
4Strain, select the neck length of spike degree more than 10cm, strain and the 9311 test cross combos of 190～210 of the straight and upright curls inward of blade, spike length 24～26cm and every fringes, and gather in the crops selected B
3F
4The selfed seed of strain obtains B
3F
5Strain;
8) plantation B
3F
5Strain all shows recessive tallness and pollen sterility; Select that sterile degree wherein reaches 100%, the strain of 190～210 of plant height 105～115cm, the straight and upright curls inward of blade, spike length 24～26cm and every fringes, be the long-spike neck sterile line of acquisition.
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|---|---|---|---|
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| CN100394843C true CN100394843C (en) | 2008-06-18 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103524608B (en) * | 2013-10-15 | 2015-05-13 | 中国水稻研究所 | Rice spike neck node regulation gene SUI1 (shorted uppermost Internode 1) and application thereof |
| CN104137767B (en) * | 2014-07-14 | 2016-01-20 | 福建金山都发展有限公司 | The selection of high resistance to cold and diseases long fringe neck CMS line |
| CN104137765B (en) * | 2014-07-14 | 2015-11-18 | 福建农林大学 | The selection of high resistance to cold and diseases long fringe neck two-line sterile line |
| CN104823841B (en) * | 2015-05-28 | 2019-02-05 | 浙江大学 | A kind of dedicated double-linear hybrid rice sterile line breeding method of starch matrix |
| CN107801629B (en) * | 2017-11-01 | 2021-01-15 | 安徽友鑫农业科技有限公司 | Breeding method of japonica two-line temperature-sensitive genic male sterile line |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1198298A (en) * | 1998-06-05 | 1998-11-11 | 福建农业大学 | Selective breeding method for ear covering free male-sterile line paddy rice |
| CN1326673A (en) * | 2001-07-24 | 2001-12-19 | 四川省农业科学院作物研究所 | Seed production method of loose-ear short-stalk long-ear-neck rice sterile line |
-
2006
- 2006-05-22 CN CNB2006100119548A patent/CN100394843C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1198298A (en) * | 1998-06-05 | 1998-11-11 | 福建农业大学 | Selective breeding method for ear covering free male-sterile line paddy rice |
| CN1326673A (en) * | 2001-07-24 | 2001-12-19 | 四川省农业科学院作物研究所 | Seed production method of loose-ear short-stalk long-ear-neck rice sterile line |
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