CN106035055A - Hybridization method for vigna radiata - Google Patents
Hybridization method for vigna radiata Download PDFInfo
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- CN106035055A CN106035055A CN201610355500.6A CN201610355500A CN106035055A CN 106035055 A CN106035055 A CN 106035055A CN 201610355500 A CN201610355500 A CN 201610355500A CN 106035055 A CN106035055 A CN 106035055A
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- emasculation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a hybridization method for vigna radiata. The method comprises the following steps: (S1) preparing tools used during hybridization, including boxes used during the collection of carina, forceps used during detasseling pollination and plastic marking plates for marking time and paternal and maternal plants after detasseling pollination; (S2) carrying out layout on a hybridization garden: carrying out strain selection on vigna radiata seedlings, dividing the vigna radiata seedlings into paternal plants and maternal plants, taking the paternal plants and the maternal plants as group, separately planting the paternal plants and the maternal plants by rows, and carrying out indicating by using the plastic marking plates for distinguishing, wherein the row spacing of the paternal plants and the maternal plants is 0.5m to 0.6m, the planting distance is 0.20m to 0.25m, and the distance among groups is 1.0m to 1.2m; and (S3) carrying out detasseling: selecting plant flower buds, which are about to blooming, of the maternal plants in the step (2), and carrying out detasseling, wherein the time for carrying out detasseling is 4:00p.m. to 7:00p.m. According to the hybridization method disclosed by the invention, the average success rate of hybridization reaches 65% or more, the number of grains per pod of hybridized pods can reach 8 or more, and the time for early education is saved, so that the hybridization method is applicable to popularization.
Description
Technical field
The present invention relates to beans hybridization technique field, particularly relate to the hybridizing method of a kind of Semen phaseoli radiati.
Background technology
Cross-breeding is the main path of artificial creation's New Crop Varieties, hybridization pollination be cross-breeding work most basic,
Most important link.Semen phaseoli radiati floral organ is little, and anthocaulus is delicate, and major part is cleisogamy, and artificial emasculation pollination is easily because of inherent, outer
Sterile in the impact of factor or come off.Therefore, hybridization difficulty is big, and success rate is low, is typically only 10%, and single pod granulating number is at 1-
Between 3.Have a strong impact on the quantity of configuration combination and effectively selected the formation of colony, and then having have impact on Breeding progress.Domestic
This is studied by outer scholars always, improves success rate.The T.B.Cuka change of the U.S. in 1986 is generally used
Boling Semen phaseoli radiati hybridizing method, uses under room temperature controllable environment, and at alabastrum abdomen ridge base portion about 2/3, strip off flower bud point, exposes post
Head and style, the method simultaneously sealing emasculation alabastrum opening with cellophane after producing Crossbred；Nineteen ninety Li Xiurong used under the previous day
Noon 4-5 point emasculation, the next morning 8-9 point smears pollination, and female parent stigma fills in fossil fragments afterwards, then with cellophane tape or face
Near leaf encases the method for alabastrum, the most not up to ideal effect.
To this end, we the hybridizing method of a kind of Semen phaseoli radiati is proposed.
Summary of the invention
The purpose of invention is to solve shortcoming present in prior art, and the hybridizing method of a kind of Semen phaseoli radiati proposed.
To achieve these goals, present invention employs following technical scheme:
The hybridizing method of a kind of Semen phaseoli radiati, comprises the steps:
S1, prepare hybridization time use instrument: include gather the box of fossil fragments, producing Crossbred time use tweezers,
Labelling time and the plastic nameplate of Parent labelling after producing Crossbred；
S2, the layout in hybridization garden: green bean-leaf is carried out roguing and green bean-leaf is divided into male parent and female parent, with male parent and female parent
It is one group, male parent and female parent are all planted by row and shown to make a distinction with plastic nameplate, the wherein row between male parent and female parent
Spacing is 0.5-0.6 rice, and spacing in the rows is 0.20-0.25 rice, and often the distance between group is 1.0-1.2 rice；
S3, emasculation: selecting the maternal plant alabastrum that will open in described S2 to carry out emasculation, the emasculation time is afternoon 4:
00-7:00；During emasculation, open vexil, ala with tweezers, break fossil fragments from alabastrum back and spill stigma and stamen, and remove
Stamen above stigma and alabastrum about, flower and all of pod；Hang up plastics board on green bean-leaf after emasculation and indicate
The emasculation date；
S4, pollination: after emasculation second day, pollination time is 6:00-10:00 in the morning；During pollination, with tweezers take off with
The fossil fragments of pollen be put in box preserve, and the fossil fragments in box are placed on the stigma of eunuchs can complete pollination；
S5, inspection: within 2-4 days after pollination, can check whether and hybridize successfully；Determine that standard is that pollination successfully flower is the longest
Going out little pod, non-hybridized successful flower comes off.
Preferably, described S3 selects the upper position of maternal plant and is in the swollen alabastrum stretching the phase and carries out emasculation.
Preferably, described S4 selects female parent be in the swollen alabastrum stretching the phase to pollinate.
Preferably, in described S2, humidity that soil to be kept is suitable and attention control pest and disease damage, and spray anti-bean-pod borer,
The medicine of thrips；Using sufficient base manure in soil, wherein base manure is based on compound fertilizer, farm organic fertilizer, according to soil fertility
Determine amount of application.
Preferably, in described S3 and S4, if maternal, string powder phenomenon occurring, available ethanol spreads upon the stigma that string powder is maternal
On carry out spermicidal.
Preferably, in the pollinating process of S4, need to first observe in fossil fragments and whether have enough fresh pollen.
The hybridizing method of a kind of Semen phaseoli radiati that the present invention proposes, during practice, this method is by emasculation, pollination
The suitableeest alabastrum cotton buds and bolls size, maternal florescence the suitableeest emasculation period, maternal plant most preferably hybridize position, emasculation Best Times and
The content of simple and easy to do hybridizing method is studied, and the hybridizing method determined makes it be hybridized to power averaging and is up to more than 65%,
And the Grain number per pod of pod of striking a bargain is up to more than 8, saves the early education time, it is suitable for promoting.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
The hybridizing method of a kind of Semen phaseoli radiati, comprises the steps:
S1, prepare hybridization time use instrument: include gather the box of fossil fragments, producing Crossbred time use tweezers,
Labelling time and the plastic nameplate of Parent labelling after producing Crossbred；
S2, the layout in hybridization garden: green bean-leaf is carried out roguing and green bean-leaf is divided into male parent and female parent, with male parent and female parent
It is one group, male parent and female parent are all planted by row and shown to make a distinction with plastic nameplate, the wherein row between male parent and female parent
Spacing is 0.5 meter, and spacing in the rows is 0.2 meter, and often the distance between group is 1 meter；
S3, emasculation: selecting the maternal plant alabastrum that will open in described S2 to carry out emasculation, the emasculation time is afternoon 4:
00；During emasculation, open vexil, ala with tweezers, break fossil fragments from alabastrum back and spill stigma and stamen, and remove on stigma
The stamen in face and alabastrum, flower and all of pod about；Hang up plastics board on green bean-leaf after emasculation and indicate emasculation day
Phase；
S4, pollination: after emasculation second day, pollination time is 6:00 in the morning；During pollination, take off band with tweezers polliniferous
Fossil fragments be put in box preserve, and the fossil fragments in box are placed on the stigma of eunuchs can complete pollination；
S5, inspection: within latter 2 days, can check whether in pollination and hybridize successfully；Determine that standard is that pollination successfully flower grows
Little pod, non-hybridized successful flower comes off.
Embodiment 2
The hybridizing method of a kind of Semen phaseoli radiati, comprises the steps:
S1, prepare hybridization time use instrument: include gather the box of fossil fragments, producing Crossbred time use tweezers,
Labelling time and the plastic nameplate of Parent labelling after producing Crossbred；
S2, the layout in hybridization garden: green bean-leaf is carried out roguing and green bean-leaf is divided into male parent and female parent, with male parent and female parent
It is one group, male parent and female parent are all planted by row and shown to make a distinction with plastic nameplate, the wherein row between male parent and female parent
Spacing is 0.53 meter, and spacing in the rows is 0.22 meter, and often the distance between group is 1.06 meters；
S3, emasculation: selecting the maternal plant alabastrum that will open in described S2 to carry out emasculation, the emasculation time is afternoon 5:
00；During emasculation, open vexil, ala with tweezers, break fossil fragments from alabastrum back and spill stigma and stamen, and remove on stigma
The stamen in face and alabastrum, flower and all of pod about；Hang up plastics board on green bean-leaf after emasculation and indicate emasculation day
Phase；
S4, pollination: after emasculation second day, pollination time is 7:00 in the morning；During pollination, take off band with tweezers polliniferous
Fossil fragments be put in box preserve, and the fossil fragments in box are placed on the stigma of eunuchs can complete pollination；
S5, inspection: within latter 2.5 days, can check whether in pollination and hybridize successfully；Determine that standard is that pollination successfully flower is the longest
Going out little pod, non-hybridized successful flower comes off.
Embodiment 3
The hybridizing method of a kind of Semen phaseoli radiati, comprises the steps:
S1, prepare hybridization time use instrument: include gather the box of fossil fragments, producing Crossbred time use tweezers,
Labelling time and the plastic nameplate of Parent labelling after producing Crossbred；
S2, the layout in hybridization garden: green bean-leaf is carried out roguing and green bean-leaf is divided into male parent and female parent, with male parent and female parent
It is one group, male parent and female parent are all planted by row and shown to make a distinction with plastic nameplate, the wherein row between male parent and female parent
Spacing is 0.56 meter, and spacing in the rows is 0.23 meter, and often the distance between group is 1.1 meters；
S3, emasculation: selecting the maternal plant alabastrum that will open in described S2 to carry out emasculation, the emasculation time is afternoon 6:
00；During emasculation, open vexil, ala with tweezers, break fossil fragments from alabastrum back and spill stigma and stamen, and remove on stigma
The stamen in face and alabastrum, flower and all of pod about；Hang up plastics board on green bean-leaf after emasculation and indicate emasculation day
Phase；
S4, pollination: after emasculation second day, pollination time is 8:00 in the morning；During pollination, take off band with tweezers polliniferous
Fossil fragments be put in box preserve, and the fossil fragments in box are placed on the stigma of eunuchs can complete pollination；
S5, inspection: within latter 3 days, can check whether in pollination and hybridize successfully；Determine that standard is that pollination successfully flower grows
Little pod, non-hybridized successful flower comes off.
Embodiment 4
The hybridizing method of a kind of Semen phaseoli radiati, comprises the steps:
S1, prepare hybridization time use instrument: include gather the box of fossil fragments, producing Crossbred time use tweezers,
Labelling time and the plastic nameplate of Parent labelling after producing Crossbred；
S2, the layout in hybridization garden: green bean-leaf is carried out roguing and green bean-leaf is divided into male parent and female parent, with male parent and female parent
It is one group, male parent and female parent are all planted by row and shown to make a distinction with plastic nameplate, the wherein row between male parent and female parent
Spacing is 0.6 meter, and spacing in the rows is 0.25 meter, and often the distance between group is 1.2 meters；
S3, emasculation: selecting the maternal plant alabastrum that will open in described S2 to carry out emasculation, the emasculation time is afternoon 6:
50；During emasculation, open vexil, ala with tweezers, break fossil fragments from alabastrum back and spill stigma and stamen, and remove on stigma
The stamen in face and alabastrum, flower and all of pod about；Hang up plastics board on green bean-leaf after emasculation and indicate emasculation day
Phase；
S4, pollination: after emasculation second day, pollination time is 9:30 in the morning；During pollination, take off band with tweezers polliniferous
Fossil fragments be put in box preserve, and the fossil fragments in box are placed on the stigma of eunuchs can complete pollination；
S5, inspection: within latter 3.5 days, can check whether in pollination and hybridize successfully；Determine that standard is that pollination successfully flower is the longest
Going out little pod, non-hybridized successful flower comes off.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope that the invention discloses, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (6)
1. the hybridizing method of a Semen phaseoli radiati, it is characterised in that comprise the steps:
The instrument used when S1, preparation hybridization: the tweezers of use, emasculation when including gathering the box of fossil fragments, producing Crossbred
Labelling time and the plastic nameplate of Parent labelling after pollination；
S2, the layout in hybridization garden: green bean-leaf is carried out roguing and green bean-leaf is divided into male parent and female parent, with male parent and female parent for one
Group, is all planted male parent and female parent by row and shows to make a distinction with plastic nameplate, the wherein line space between male parent and female parent
For 0.5-0.6 rice, spacing in the rows is 0.20-0.25 rice, and often the distance between group is 1.0-1.2 rice；
S3, emasculation: selecting the maternal plant alabastrum that will open in described S2 to carry out emasculation, the emasculation time is 4:00-7 in afternoon:
00；During emasculation, open vexil, ala with tweezers, break fossil fragments from alabastrum back and spill stigma and stamen, and remove on stigma
The stamen in face and alabastrum, flower and all of pod about；Hang up plastics board on green bean-leaf after emasculation and indicate emasculation day
Phase；
S4, pollination: after emasculation second day, pollination time is 6:00-10:00 in the morning；During pollination, take off with pollen with tweezers
Fossil fragments be put in box preserve, and the fossil fragments in box are placed on the stigma of eunuchs can complete pollination；
S5, inspection: within 2-4 days after pollination, can check whether and hybridize successfully；Determine that standard is that pollination successfully flower has grown little
Pod, non-hybridized successful flower comes off.
The hybridizing method of a kind of Semen phaseoli radiati the most according to claim 1, it is characterised in that select maternal plant in described S3
Upper position is also in the swollen alabastrum stretching the phase and carries out emasculation.
The hybridizing method of a kind of Semen phaseoli radiati the most according to claim 1, it is characterised in that select female parent to be in swollen in described S4
The alabastrum stretching the phase is pollinated.
The hybridizing method of a kind of Semen phaseoli radiati the most according to claim 1, it is characterised in that in described S2, soil to be kept is fitted
When humidity and attention control pest and disease damage, and spray the medicine of anti-bean-pod borer, thrips；Base manure, wherein base manure is used sufficient in soil
Based on compound fertilizer, farm organic fertilizer, determine amount of application according to soil fertility.
The hybridizing method of a kind of Semen phaseoli radiati the most according to claim 1, it is characterised in that in described S3 and S4, if maternal
String powder phenomenon occurs, and available ethanol spreads upon and carries out spermicidal on the stigma that string powder is maternal.
The hybridizing method of a kind of Semen phaseoli radiati the most according to claim 1, it is characterised in that in the pollinating process of S4, need elder generation
Observe in fossil fragments and whether have enough fresh pollen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610355500.6A CN106035055A (en) | 2016-05-25 | 2016-05-25 | Hybridization method for vigna radiata |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610355500.6A CN106035055A (en) | 2016-05-25 | 2016-05-25 | Hybridization method for vigna radiata |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853171A (en) * | 2017-12-04 | 2018-03-30 | 柏绿山 | One kind food leaf grass and its implantation methods |
| CN109105250A (en) * | 2018-08-06 | 2019-01-01 | 重庆市农业科学院 | A kind of efficient mung bean cross breeding method |
| CN109105254A (en) * | 2018-09-25 | 2019-01-01 | 中国科学院东北地理与农业生态研究所 | A method of improving soybean hybridization efficiency |
| CN116210583A (en) * | 2021-08-23 | 2023-06-06 | 吉林省农业科学院 | Breeding method of vertical green bean pod multi-pod long-grain large new germplasm |
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2016
- 2016-05-25 CN CN201610355500.6A patent/CN106035055A/en active Pending
Non-Patent Citations (2)
| Title |
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| 刘玉平等: ""关于绿豆杂交方法的探讨"", 《河北农业科学》 * |
| 马锡伯等: ""浅谈绿豆育种及产量构成"", 《辽宁农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853171A (en) * | 2017-12-04 | 2018-03-30 | 柏绿山 | One kind food leaf grass and its implantation methods |
| CN109105250A (en) * | 2018-08-06 | 2019-01-01 | 重庆市农业科学院 | A kind of efficient mung bean cross breeding method |
| CN109105254A (en) * | 2018-09-25 | 2019-01-01 | 中国科学院东北地理与农业生态研究所 | A method of improving soybean hybridization efficiency |
| CN116210583A (en) * | 2021-08-23 | 2023-06-06 | 吉林省农业科学院 | Breeding method of vertical green bean pod multi-pod long-grain large new germplasm |
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Application publication date: 20161026 |