CN102388798A - Breeding method of rice photo-thermo-sensitive genic male sterile line - Google Patents
Breeding method of rice photo-thermo-sensitive genic male sterile line Download PDFInfo
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- CN102388798A CN102388798A CN2011102135160A CN201110213516A CN102388798A CN 102388798 A CN102388798 A CN 102388798A CN 2011102135160 A CN2011102135160 A CN 2011102135160A CN 201110213516 A CN201110213516 A CN 201110213516A CN 102388798 A CN102388798 A CN 102388798A
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Abstract
The invention discloses a breeding method of a rice photo-thermo-sensitive genic male sterile line, belonging to a breeding method of plant seeds. The breeding method comprises the following steps of: (1) selecting an early-ripe indica rice photo-thermo-sensitive genic male sterile line Peiai 64S as a female parent and selecting an early-ripe indica rice photo-thermo-sensitive genic male sterile line Guangzhan 63S as a male parent; hybridizing to obtain F1 seeds; and (2) soaking the F1 seeds, accelerating germination and sowing; carrying out selfing for six generations and selecting a photo-thermo-sensitive genic male sterile line target variety with dense panicle, colorless glume end, short straw and strong lodging resistance. The breeding method provided by the invention has the advantages of moderate plant distribution, short and healthy plants and strong lodging resistance, good out-crossing characteristic, and colorless leaf sheath, glume end and stigma; and according to the breeding method, the purity identification can be easily finished.
Description
Technical field
The present invention relates to a kind of breeding method of plant seed, more specifically to a kind of breeding method of photo-thermo sensitive male nuclear sterile rice line.
Background technology
Paddy rice is the first crops of China, and hybrid rice output is high, wide adaptability, and cultivation easily, cultivated area accounts for 50% of the national paddy rice gross area, and output accounts for 60% of paddy rice gross yield, and applying to China's grain-production of hybrid rice makes a great contribution.China's double-hybrid rice strains high-tech that to create in order to using up temperature sensitive nuclear sterility again be main contents after the Three-line Hybrid paddy rice.Compare with the Three-line Hybrid rice breeding, the double-hybrid rice strains breeding has significant superiority: the one, and male sterile line and recovery are that combo is free, the probability of seed selection fine combination increases; The 2nd, male sterile line one is dual-purpose, under long day hot conditions (summer) can be used for the production of hybrid seeds, under short day cryogenic conditions (spring, autumn) can be used for the breeding of self, need be by maintenance line (two is not to come therefrom).Therefore can simplify breeding, production of hybrid seeds program reduces the seed production cost; The 3rd, because the temperature sensitive sterility nuclear gene heredity of light is irrelevant with cytoplasm, can overcome cytoplasmic negative effect in the three line method male sterile line; The 4th, can open up subspecies indica and japonica hybrid advantage frontier immediately following the seed selection paces of conventional rice, make rice yield on existing hybrid rice basis, realize more high yield target.
Over past ten years, China's double-hybrid rice strains breeding makes much progress, and large quantities of double-hybrid rice strains new varieties are bred in succession and on producing, applied.But it is perfect not enough that existing hybridization is kind, and the lodging resistance that has is not enough, and the fringe type that has is loose, and the clever sharp purple that has, true and false hybrid are difficult to distinguish, and purity is identified relatively difficulty.Therefore, cultivate the dense cluster that makes new advances, the grain husk point is colourless, and the short strong photo-thermo-sensitive genic male sterile line of plant has significant application value in the double-hybrid rice strains breeding.
Summary of the invention
Goal of the invention: the purpose of this invention is to provide a kind of breeding method of photo-thermo sensitive male nuclear sterile rice line, cultivate dense cluster type, colourless, of short stem, the anti-photo-thermo-sensitive genic male sterile line paddy rice of grain husk point.
Technical scheme: for realizing above-mentioned purpose, the breeding method of a kind of photo-thermo sensitive male nuclear sterile rice line of the present invention comprises the steps:
(1) select in close, the clever sharp purple of fringe type, plant type is compact, lodging resistance the is strong precocity indica type photo-thermo-sensitive genic male sterile line to train short 64S as female parent; Select that indica type photo-thermo-sensitive genic male sterile line extensively accounts for 63S as male parent in the precocity best in quality, that colourless, the different friendship characteristic of grain husk point is good, hybridization obtains F
1Seed;
(2) with F
1Seed presoaking and germinating sowing, through the selfing of 6 generations, select that the fringe type is close, colourless, the short bar of grain husk point, photo-thermo-sensitive genic male sterile line target variety that lodging resistance is strong.
Wherein, said female parent selection is annotated in the precocity that leaf attitude is good, coordinate force is high, different friendship characteristic is good indica type photo-thermo-sensitive genic male sterile line and is trained short 64S;
The said selfing of step (2) is from F
2Generation begins to select that the fringe type is close, the grain husk point is colourless, short strong, different friendship characteristic is good, the fertility inversion temperature the is low photoperiod-temperature sensitive genie male-sterile line individual plant of plant.
The said selfing of step (2) is from F
3In generation, begin to carry out the resistance evaluation and screening.
Beneficial effect: the breeding method moderate growth duration of a kind of photo-thermo sensitive male nuclear sterile rice line provided by the invention, plant type is collecting and distributing moderate, tillering ability is strong, plant short strong, lodging resistance is strong, different friendship characteristic is good, the production of hybrid seeds is easy, effectively fringe is many, is beneficial to pollen transmission.Owing to rice leaf sheath, grain husk point, column cap that the present invention cultivates are all colourless, using this male sterile line and leaf sheath, clever recovery sharp, that column cap is purple is the production of hybrid seeds, hybrid F
1Leaf sheath, grain husk point, column cap must show as purple, after the hybrid seed results, through indoor germination test, wherein to show as the plant of purple be true hybrid for bud scale, leaf sheath, and wherein bud scale, the colourless plant of leaf sheath are pseudostationary.
Embodiment
Embodiment
(1) 2003 year spring; In Hainan; Selecting in close, the clever sharp purple of fringe type, plant type is compact, the short strong lodging resistance of plant is strong, coordinate force is high, different friendship characteristic the is good precocity indica type photo-thermo-sensitive genic male sterile line to train short 64S does maternal; Extensively account for 63S hybridization with indica type photo-thermo-sensitive genic male sterile line in the good precocity of colourless, the different friendship characteristic of best in quality, clever point, obtain F
143 in seed.
(2) 2003 year summer is in Zhenjiang, with F
1Seed presoaking and germinating, vacation, 3 strains of bad hybrid are eliminated in sowing, and all the other 17 strains mix receives.
(3) 2004 year summer transplanted F in Zhenjiang
21096 strains according to 15 individual plants of comprehensive agronomy character screening, are gathered in the crops F November
3Seed.
(4) 2005 year spring eliminated 7 strain systems, elected 12 individual plants in Hainan with F3.
(5) 2005 year summer eliminated 6 strain systems, elected 9 individual plants in Zhenjiang with F4.
(6) 2006 year spring eliminated 4 strain systems, elected 10 individual plants in Hainan.
(7) 2006 year summer be in Zhenjiang, selects 6 strains systems and raise No. 6 surveys of rice and join.
(8) 2007 year spring be in Hainan, select 4 strains systems once more with raise No. 6 survey of rice and join, the while breeding seed obtains target variety.
Management method during the breeding is following:
1, the base is selected
When adopting distance isolation, require isolation distance to be no less than 700m, forbid on every side 1000m with interior plantation round-grained rice, glutinous rice variety; When adopting barrier to isolate, require barrier height to reach about 4m, reach in flakes more than the 200m; The employing time can not be less than 25 days with the different kind of the maintenance line time interval at florescence when isolating.The field piece is concentrated in flakes, fertile soil, and irrigation and drainage are convenient, do not have the insect pest of quarantine venereal disease.
2, date of seeding and plant specification
The Yangtze river basin autumn is numerous, goes up general July, sowing in the middle ten days, and numerous general late October to early November in Hainan spring is sowed, numerous still numerous individual plant transplanting that all requires of Hainan spring of continent autumn no matter, and seeding row spacing is controlled at (12～15) cm * (16～19) cm.
3. rich water is planned strategies for
Fertilizer and inorganic fertilizer collocation are used, and are main with fertilizer.General mu is executed pure N12.0～15.0 kilogram, and N, P, K proportioning are 1:0.8:0.5.On fertilizer application, basal plane fertilizer 60%, tillering fertilizer 25～30%, balance fertilizer 10～15%, the intermediary and later stages are generally controlled N fertilizer and use except that being used for the florescence adjusting.Water starch management adopts the shallow water rice transplanting, and deep water is lived, and shallow water is diligent irritates short and early send out, and (18～200,000 seedling/mu) was in time put the field when total stem tiller number reached predetermined spike number, heading flowering period maintenance shoaling layer, and the later stage prevents to cut off the water supply too early.
4. anti-cure the disease, worm, crop smothering
According to disease, worm, crop smothering pests occurrence rule, prevent curing rice thrips, snout moth's larva, rice fulgorid, banded sclerotial blight, and carry out the preventing and controlling of seedling, big field weed.Emphasis is done a good job of it the preventing and controlling of rice kernel smut, false smut.
Through statistics, in 125 ~ 135 days time of infertility of this kind, sowing on May 11 is broadcast neat fate 89 days, 17.0 in the total blade of stem in the Jurong, Jiangsu; May 21, sowing was broadcast neat fate 85 days, 16.0 in the total blade of stem.The short 64S of training that broadcasts the sowing of neat fate and stem total leaf number and the same period is suitable.Plant type is collecting and distributing moderate, and tillering ability is strong, and the percentage of earbearing tiller is high, and radical leaves leaf angle is bigger, and upper blade is tall and straight, and the leaf look moderate, and the stem stalk is sturdy, and lodging resistance is strong.Single 4～5 days Honoka phases, bloom 7～8 days phases of going through in single cave, goes out the sheath grain husk the flowers are in blossom clever rate 86.78%; Go out the sheath grain husk and spend and open clever rate in 2 days and reach 75.64%, bag neck granule rate 28.6% is to " 92 〇 " sensitivity; The bag neck can be removed with 15～20g " 92 〇 " in the production of hybrid seeds, breeding field.Concentrate on the peak of blooming, and flower accounted for and opened grain husk sum 58% same day morning, went out sheath grain husk chapiter exposing ratio 86.76%, wherein monolateral exposing ratio 34.22%, bilateral exposing ratio 52.52%; Spray and go out sheath grain husk chapiter exposing ratio after " 92 〇 " and can reach 98.54%, wherein monolateral exposing ratio 31.30%, bilateral exposing ratio 67.24%.Column cap reaches, long 3.00mm, and wide 1.0mm helps catching the pollen of male parent.
The rice varieties that utilizes method provided by the invention to cultivate, leaf sheath, grain husk point, column cap are all colourless, are the production of hybrid seeds with this male sterile line and leaf sheath, grain husk is sharp, column cap is purple recovery, hybrid F
1Leaf sheath, grain husk point, column cap must show as purple, after the hybrid seed results, through indoor germination test, wherein to show as the plant of purple be true hybrid for bud scale, leaf sheath, and wherein bud scale, the colourless plant of leaf sheath are pseudostationary.
The above only is a preferred implementation of the present invention; Be noted that for those skilled in the art; Under the prerequisite that does not break away from the principle of the invention, can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (3)
1. the breeding method of a photo-thermo sensitive male nuclear sterile rice line is characterized in that comprising the steps:
(1) select in close, the clever sharp purple of fringe type, plant type is compact, lodging resistance the is strong precocity indica type photo-thermo-sensitive genic male sterile line to train short 64S as female parent; Select that indica type photo-thermo-sensitive genic male sterile line extensively accounts for 63S as male parent in the precocity best in quality, that colourless, the different friendship characteristic of grain husk point is good, hybridization obtains F
1Seed;
(2) with F
1Seed presoaking and germinating sowing, through the selfing of 6 generations, select, cultivate that the fringe type is close, colourless, the short bar of grain husk point, photo-thermo-sensitive genic male sterile line target variety that lodging resistance is strong.
2. the breeding method of a kind of photo-thermo sensitive male nuclear sterile rice line according to claim 1, it is characterized in that: the said selfing of step (2) is from F
2Generation begins to select that the fringe type is close, the grain husk point is colourless, short strong, different friendship characteristic is good, the fertility inversion temperature the is low photoperiod-temperature sensitive genie male-sterile line individual plant of plant.
3. the breeding method of a kind of photo-thermo sensitive male nuclear sterile rice line according to claim 1, it is characterized in that: the said selfing of step (2) is from F
3In generation, begin to carry out the resistance evaluation and screening.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960235A (en) * | 2012-10-24 | 2013-03-13 | 北京金色农华种业科技有限公司 | Production method of hybrid rice seed Guangliangyou 674 |
| CN103598085A (en) * | 2013-10-28 | 2014-02-26 | 江苏沿海地区农业科学研究所 | Breeding method of photo-thermo-sensitive genic male sterile line of quality rice |
| CN103960119A (en) * | 2013-11-06 | 2014-08-06 | 仲恺农业工程学院 | Method for breeding PTGMS (photo-thermo-sensitive genie male sterile) line with longer-term low temperature resistance |
| CN104663423A (en) * | 2015-02-05 | 2015-06-03 | 广德县农业科学研究所 | Method for breeding thermo-photoperiod sensitive dual-purpose genic male sterile Guangxuan S |
| CN105941128A (en) * | 2016-03-16 | 2016-09-21 | 安徽省农业科学院水稻研究所 | Method of selectively breeding dwarf indica rice nuclear sterile line with pyramided minor dominant gene |
| CN106879451A (en) * | 2017-03-01 | 2017-06-23 | 广东省农业科学院水稻研究所 | A kind of improved paddy rice chemical emasculation method breeding method |
| CN108476969A (en) * | 2018-02-12 | 2018-09-04 | 贵州省农作物品种资源研究所 | Rice homogeneity oozes the selection of round-grained rice restorer |
| CN110050685A (en) * | 2019-04-09 | 2019-07-26 | 武汉弘耕种业有限公司 | A kind of seed choosing method of photo-thermo sensitive male nuclear sterile rice line |
| CN111543314A (en) * | 2020-06-29 | 2020-08-18 | 四川发生种业有限责任公司 | Breeding method of high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line |
| CN112753564A (en) * | 2020-12-29 | 2021-05-07 | 湖南省春云农业科技股份有限公司 | Breeding method of high-quality two-line sterile line of rice |
-
2011
- 2011-07-28 CN CN2011102135160A patent/CN102388798A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960235A (en) * | 2012-10-24 | 2013-03-13 | 北京金色农华种业科技有限公司 | Production method of hybrid rice seed Guangliangyou 674 |
| CN102960235B (en) * | 2012-10-24 | 2014-02-26 | 北京金色农华种业科技有限公司 | Production method of hybrid rice seed Guangliangyou 674 |
| CN103598085A (en) * | 2013-10-28 | 2014-02-26 | 江苏沿海地区农业科学研究所 | Breeding method of photo-thermo-sensitive genic male sterile line of quality rice |
| CN103598085B (en) * | 2013-10-28 | 2015-09-23 | 江苏沿海地区农业科学研究所 | A kind of selection of photo-thermo sensitive male nuclear sterile rice line |
| CN103960119A (en) * | 2013-11-06 | 2014-08-06 | 仲恺农业工程学院 | Method for breeding PTGMS (photo-thermo-sensitive genie male sterile) line with longer-term low temperature resistance |
| CN104663423A (en) * | 2015-02-05 | 2015-06-03 | 广德县农业科学研究所 | Method for breeding thermo-photoperiod sensitive dual-purpose genic male sterile Guangxuan S |
| CN105941128A (en) * | 2016-03-16 | 2016-09-21 | 安徽省农业科学院水稻研究所 | Method of selectively breeding dwarf indica rice nuclear sterile line with pyramided minor dominant gene |
| CN106879451A (en) * | 2017-03-01 | 2017-06-23 | 广东省农业科学院水稻研究所 | A kind of improved paddy rice chemical emasculation method breeding method |
| CN108476969A (en) * | 2018-02-12 | 2018-09-04 | 贵州省农作物品种资源研究所 | Rice homogeneity oozes the selection of round-grained rice restorer |
| CN110050685A (en) * | 2019-04-09 | 2019-07-26 | 武汉弘耕种业有限公司 | A kind of seed choosing method of photo-thermo sensitive male nuclear sterile rice line |
| CN111543314A (en) * | 2020-06-29 | 2020-08-18 | 四川发生种业有限责任公司 | Breeding method of high-quality, high-yield and disease-resistant rice photo-thermo-sensitive genic male sterile line |
| CN112753564A (en) * | 2020-12-29 | 2021-05-07 | 湖南省春云农业科技股份有限公司 | Breeding method of high-quality two-line sterile line of rice |
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Application publication date: 20120328 |