CN112673954B - High-yield breeding method of sesame with limited growth habit - Google Patents
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Abstract
The invention provides a high-yield breeding method of limited growth habit sesame, which comprises the following steps: s1, sowing the three capsule type sesame germplasm resources according to the row spacing of more than or equal to 40cm, and thinning in the true leaf stage at 1-2 times, wherein the seedling spacing is 3-5 cm; performing final singling at the true leaf stage at 3-4, wherein the seedling spacing is more than or equal to 30 cm; s2, screening sesame plants of the pluea carpea; s3, carrying out artificial hybridization by taking the multi-capsule sesame plant obtained in the step S2 and the sesame with limited growth habit as parents, and harvesting F1 generation hybrid seeds; s4, planting and selecting filial generation; the improvement of the limited growth habit sesame varieties can be completed, the per-axillary capsule forming number and the per-plant capsule number of the limited growth habit sesame varieties are greatly increased, the single yield of the limited growth habit sesame is greatly improved, the bottleneck of low single yield of the limited growth habit sesame is broken, and the limited growth habit sesame varieties are expected to be applied in actual production.
Description
Technical Field
The invention relates to the field of sesame breeding, in particular to a high-yield breeding method of sesame with limited growth habit.
Background
Sesame (s.indicum l., 2n ═ 26) is an important traditional high-quality oil crop in China and is also one of the ancient oil crops in the world. Sesame seeds have high nutritional value and wide consumer groups, and are indispensable raw materials for many traditional foods in China. Sesame seeds are high in oil content, rich in unsaturated fatty acids (wherein oleic acid and linoleic acid account for about 85% of the total amount), and also contain natural antioxidant substances such as sesamin (sesamin), sesamol (sesamol), vitamin E and the like, so that the sesame seeds are very beneficial to human health and are important high-quality edible oil sources and nutritional health-care foods.
The common cultivated sesame varieties have unlimited growth habits, the flowering period of the sesame varieties is 30-40d, and the sesame varieties are even longer, can adapt to changeable climatic environments, and are beneficial to the self survival of the species. But the unlimited growth habit greatly influences the yield, the quality, the mechanized harvesting and the like of the sesame in the production and utilization. In the later growth stage of sesame, the dry weight of the hollow tip or yellow tip at the top of a plant accounts for about 5 percent of the total weight of dry matters of a mature single plant due to the unlimited growth habit, so that the ineffective consumption of sesame biomass is increased, and the exertion of the yield potential of seeds of the single plant is reduced. The excessive flowering period causes the maturation inconsistency of capsules and seeds at different parts, the top flowering period is not finished, the bottom capsule is mature and begins to burst, the reasonable harvesting time is difficult to determine, the reduction and loss of seed yield can be caused by early or late harvesting, and the application of the mechanical harvesting of sesame is severely restricted. Meanwhile, the inconsistent maturity of the seeds is also an important reason for influencing the quality traits of sesame oil content, seed color and the like, the oil content, the protein content and the fatty acid composition of the seeds with inconsistent maturity are obviously different, the seed color is also related to the maturity, particularly the color of the black sesame seeds, the color difference of the black sesame seeds with inconsistent maturity is larger, even the seed skin melanin is not as good as the accumulated white seeds and yellow seeds, the appearance quality of the black sesame is seriously influenced, and the bottleneck problem of restricting the production of the black sesame, particularly the export quality is one. In a word, the development and breakthrough of sesame production and cultivation system reformation are restricted to a certain extent by the infinite growth habit. At present, the main method for overcoming the problem is to artificially top the sesame at the later stage, remove the growing point of the stem tip and inhibit unlimited growth, and the existing research shows that proper-period top-cutting can limit the meristem of the hollow tip, reduce ineffective consumption, realize yield increase of 9.8-17.6% and improve the commodity grade by 1-2 grades, but the technology has the defects of difficult control of top-cutting time, time and labor consumption and the like, and is difficult to produce and utilize in a large scale. Since the limited mutants appear, the breeding of sesame varieties with limited growth habits has become one of the most ideal ways to overcome the problems caused by the unlimited growth, and the creation and breeding of sesame mutant materials with limited growth habits has also been an important direction for sesame research.
However, sesame with limited growth habit has fixed capsule axillary node number, and the phenomenon of ceiling is existed in the single capsule number, thus the improvement of the sesame yield with limited growth habit is seriously limited, and the application of sesame variety with limited growth habit is seriously limited. Therefore, the high-yield breeding method of the limited growth habit sesame is established, and the number of capsules per axillary capsule is greatly increased on the basis of relatively fixed number of axillary nodes, so that the number of single-plant capsules and the yield of a single plant are greatly increased, the high-yield target of the limited growth habit sesame is achieved, and technical support is provided for production and application of the limited growth habit sesame.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the defect of low yield of sesame with limited growth habit in the prior art, thereby providing a high-yield breeding method of sesame with limited growth habit.
The invention provides a high-yield breeding method of sesame with limited growth habit, which comprises the following steps:
s1, sowing the three capsule type sesame germplasm resources according to the row spacing of more than or equal to 40cm, thinning in the true leaf stage at 1-2, wherein the seedling spacing is 3-5 cm; fixing seedlings in the true leaf stage at 3-4 times, wherein the seedling spacing is more than or equal to 30 cm;
s2, observing and recording the flowering condition of the sesame plants in the step S1 after the sesame plants enter the full-bloom stage, and marking the germplasm resources with the axillary flowering number of each leaf being more than 3; investigating and recording the capsule condition after final flowering, and marking the germplasm resources with the number of capsules per axilla being more than 3; after the plants are mature, germplasm resource markers with the axillary flowering number and the capsule number larger than 3 are subjected to seed test analysis, sesame plants with S capsules growing in each axillary are selected from more than or equal to 50% of nodes in the middle of the plants, and S is more than or equal to 5 and less than or equal to 7, so that the sesame germplasm with multiple capsules is obtained;
S3, carrying out artificial hybridization by taking the germplasm of the plutella xylostella obtained in the step S2 and the sesame with limited growth habit as parents, and harvesting F1 generation hybrid seeds;
s4, planting F1 generation hybrid seeds to obtain F1 plants, and after the F1 plants bloom, carrying out mixed harvesting on plants with the number of capsules per axillary capsule more than or equal to 5 in more than or equal to 50% of the nodes in the middle of the F1 plants to obtain F2 generation seeds;
s5, planting F2 generation seeds to obtain an F2 separation population, after the plants of the F2 separation population bloom and capsula, selecting individual plants which have the number of capsules per axillary capsule of more than or equal to 50% of the nodes in the middle of the plants and show limited growth habit, marking and numbering the individual plants, and harvesting the individual plants after maturation to obtain F3 generation seeds; then planting the seeds of the F3-F6 generation according to the method, selecting a plant line which has the characteristics that the number of capsules per leaf in the section which is more than or equal to 50 percent of the sections in the middle of the plant and the limited growth habit are simultaneously numbered, and harvesting the plants according to the single plant after the plants are mature; and finally planting the generation seeds above F7 and harvesting in a mixing way.
In the invention, the plant comprises an upper part (with a height of a), a middle part (with a height of b) and a lower part (with a height of c) from top to bottom, the length of the whole plant is H, and the ratio of b to H is more than or equal to 1/3.
Optionally, in step S1, the row spacing is 40-60cm, and the row spacing after final singling is 30-40 cm.
Optionally, the hybrid is planted in a manner of sparse planting.
Optionally, the thin planting method is as follows: sowing the hybrid seeds according to the row spacing of more than or equal to 40cm, thinning the seedlings at the true leaf stage by 1-2 pairs, and keeping the seedling spacing between 3-5 cm; and (4) final singling is carried out in the true leaf stage at 3-4, and the seedling spacing is more than or equal to 30 cm.
Optionally, the thin planting method is as follows: the row spacing for planting the hybrid seeds is 40-60cm, and the seedling spacing after final singling is 30-40 cm.
Optionally, the number of the plants of each generation of segregating population obtained by artificial hybridization is more than or equal to 30.
Optionally, the artificial hybridization is: the artificial emasculation is carried out on one of the parents in the evening of the previous day, and then pollen of the flower which is opened by the other parent is taken in the morning of the next day to pollinate the pistil of the parent after the artificial emasculation.
The technical scheme of the invention has the following advantages:
1. the invention provides a high-yield breeding method of limited growth habit sesame, which comprises the following steps: s1, sowing the three capsule type sesame germplasm resources according to the row spacing of more than or equal to 40cm, and thinning in the true leaf stage at 1-2 times, wherein the seedling spacing is 3-5 cm; performing final singling at the true leaf stage at 3-4, wherein the seedling spacing is more than or equal to 30 cm; s2, observing and recording the flowering condition of the sesame plants in the step S1 after the sesame plants enter the full-bloom stage, and marking the germplasm resources with the axillary flowering number of each leaf being more than 3; investigating and recording the capsule condition after final flowering, and marking the germplasm resources with the number of capsules per axilla being more than 3; after the plants are mature, germplasm resource markers with the axillary flowering number and the capsule number larger than 3 are subjected to seed test analysis, sesame plants with S capsules growing in each axillary are selected from more than or equal to 50% of nodes in the middle of the plants, and S is more than or equal to 5 and less than or equal to 7, so that the sesame germplasm with multiple capsules is obtained; s3, carrying out artificial hybridization by taking the multi-capsule sesame germplasm and the sesame with limited growth habit obtained in the step S2 as parents, and harvesting F1 generation hybrid seeds; s4, planting F1 generation hybrid seeds to obtain F1 plants, and after the F1 plants bloom, carrying out mixed harvesting on plants with the number of axillary capsules of each node more than or equal to 5 and more than or equal to 50% of the nodes in the middle of the F1 plants to obtain F2 generation seeds; s5, planting F2 generation seeds to obtain an F2 separation group, after the plants of the F2 separation group bloom and capsize, selecting single plants which have more than or equal to 50% of the nodes in the middle of the plants, have more than or equal to 5 axillary capsules per node and show limited growth habits, marking and numbering, and harvesting according to the single plants after maturation to obtain F3 generation seeds; then planting seeds of F3-F6 generation according to the method, selecting a plant line which has the characteristics of more than or equal to 5 per axillary capsule number of the node of more than or equal to 50% of the nodes in the middle of the plant and limited growth habit at the same time, numbering, and harvesting according to the single plant after maturation; finally, planting the generation seeds above F7 and harvesting in a mixed manner; since sesame with limited growth habit has fixed number of axillary nodes and the characteristics of each axillary polycapsule of the three-capsule type sesame have extremely low occurrence rate and extremely low stability of characteristic expression, researchers neglect the characteristics of each axillary polycapsule as the improvement characteristics of sesame with limited growth habit in the aspect of improving the yield of sesame with limited growth habit, and there is no report on the improvement characteristics of each axillary polycapsule of the related sesame with limited growth habit and the success, since the three-capsule type sesame is a single-rod type sesame, is not usually branched and is suitable for close planting, the researchers have not found that low-density planting will affect the stability of the occurrence rate and the characteristic expression of each axillary polycapsule of the three-capsule type sesame, and the applicants found that the occurrence rate and the stability of the characteristic expression of each axillary polycapsule of the three-capsule type sesame can be obviously improved when the three-capsule type sesame is implemented according to the step S1 in the research process, can efficiently obtain the per-axillary multi-capsule character germplasm resources of the three-capsule type sesame, and provides new favorable character gene resources for the genetic improvement of the sesame; through the step S2, each axillary multi-capsule trait germplasm resource of the three-capsule type sesame can be effectively screened; through the breeding method of the steps S3-S5, the improvement of the limited growth habit sesame variety can be completed, the number of capsules formed in each axilla and the number of capsules in each individual plant of the limited growth habit sesame variety are greatly increased, the single yield of the limited growth habit sesame is greatly improved, the bottleneck of low single yield of the limited growth habit is broken, and the limited growth habit sesame variety is expected to be applied to actual production.
Detailed Description
The following examples are provided to better understand the present invention, not to limit the best mode, and not to limit the content and protection scope of the present invention, and any product that is the same or similar to the present invention and is obtained by combining the present invention with other features of the prior art and the present invention falls within the protection scope of the present invention.
The examples do not indicate specific experimental procedures or conditions, and can be performed according to the procedures or conditions of the conventional experimental procedures described in the literature in the field. The reagents or instruments used are conventional reagent products which are commercially available, and manufacturers are not indicated.
The evening time period referred to in the following examples is from 16:00 to 19:00 and the morning time period is from 7:00 to 9: 00.
Example 1
The materials used in this example are 107 tripaque sesame germplasm resources (germplasm resources are provided by crop institute of agricultural academy of sciences of the Jiangxi province and the crop center of the Jiangxi province, germplasm resources are shown in Table 2, and part of germplasm resources are named and numbered by crop institute of agricultural academy of the Jiangxi province and the crop institute of agricultural academy of the Jiangxi province) and a sesame line with limited growth habit "NFS 23" (provided by crop germplasm resources center of the Jiangxi province and the crop institute of agricultural academy of the Jiangxi province and named by number), "NFS 23" is obtained by hybridizing Ganzhi 14 with sesame DS899 with sesame with limited growth habit, and the breeding method is that Ganzhi 14 is used as a female parent, DS899 is used as a male parent, and is artificially hybridized with continuous generations (F2-F8), each plant with limited growth habit is selected, and the DS parent strain 899 is introduced from sesame research center of agricultural academy of the Henan province, and the specific operation steps are as follows:
(1) Sparse planting identification of three capsule type sesame germplasm resources. Selecting a land suitable for sesame planting, sowing 107 parts of sesame germplasm resources at a row spacing of 40-60cm (in the embodiment, a row spacing of 40cm is selected), thinning at a main leaf stage of 1-2, and requiring a seedling spacing of 3-5cm (in the embodiment, a seedling spacing of 3cm is selected); performing final singling at the true leaf stage at 3-4, wherein the plant spacing is 30-40cm (in the embodiment, 30cm row spacing is selected); and observing and recording the properties of the capsules after the full-bloom period of the plants. Other field management is basically consistent with that of normal sesame planting.
(2) Screening the variant types of the pluea capsulata. Observing and recording the flowering condition of the plants after the sesame germplasm resources sparsely planted in the step (1) enter the full-bloom stage, and marking the germplasm resources with the axillary flowering number of more than 3; surveying and recording the capsule condition again after final flowering, and marking the germplasm resources with the effective capsule number of each axilla being more than 3; and (4) after the plants are mature, performing seed test analysis on the germplasm resource markers with the axillary flowering number and the capsule number of each leaf larger than 3. 107 portions of the resources, the flowering number of each axillary is between 1.0 and 4.35, the average capsule forming number of each axillary is between 1.0 and 3.66, and sesame germplasm resources meeting the requirement that more than or equal to 50 percent of nodes in the middle of a plant are positioned to grow 5 to 7 capsules are selected from the resources, in the sesame germplasm resource meeting the requirement that more than or equal to 50 percent of nodes in the middle of the plant are occupied with 5-7 capsules, "RX 786" (provided by Jiangxi crop germplasm resource center & Jiangxi agricultural science institute and named by number) is the optimal sesame germplasm resource of the multi-capsule, specifically, 1-5 capsules are planted in the upper and lower axilla, the average flowering number of the whole plant per axilla is 4.35, the average capsule forming number of the whole plant per axilla is 3.66, the average single capsule number is 300.11, and the average single yield is 41.51g, so that RX786 is selected as the sesame germplasm resource of the multi-capsule in the embodiment.
(3) And (4) artificial hybridization. And (3) identifying and screening the obtained pluriclosa maxima sesame "RX 786" and the sesame with limited growth habit "NFS 23" as parents by utilizing the step (2) and carrying out artificial hybridization. The specific method comprises the following steps: artificial castration is carried out on one of the parents at 16:00-19:00 in the evening of the first day, pollen of the flower which is opened by the other parent is taken at 7:00-9:00 in the morning of the next day, and the pistil of the parent which is subjected to artificial castration is pollinated.
(4) And (4) planting and selecting filial generation. And (3) sparsely planting the seeds (F1 generation hybrid seeds) harvested after hybridization in the step (3) (the line spacing, the thinning seedling spacing and the final seedling spacing are the same in the step (1)) in the soil suitable for sesame planting, and after the flowering seeds of the F1 plants are mature, mixing and harvesting the multi-capsule plants with the number of axillary capsules of each node being more than or equal to 5 and more than or equal to 50% of the nodes in the middle of the plants to obtain the F2 generation seeds. And then, thinly planting seeds (2 generation seeds) harvested from the F1 plant to obtain an F2 segregation population, selecting single plants which have the axillary capsule number of more than or equal to 50% of nodes in the middle of the plant and show limited growth habits for marking and numbering after the plants bloom and capsula, and harvesting according to the single plants after the plants are mature. And then, continuously carrying out thin planting on the F3-F6 generation plant lines according to the method for thin planting in the step (1), wherein the row spacing, the thinning seedling spacing and the final singling seedling spacing are the same, selecting the plant lines which have the characteristics of the number of axillary capsules per leaf of greater than or equal to 50% of the nodes in the middle of the plant and the limited growth habit, numbering the plant lines, and considering the characteristics of plant line resistance, seed quality and the like. Mixed harvest was then carried out on plants of limited growth habit of the polycarpa in generations above F7 (generation F7 was selected in this example).
Identifying the stress resistances of various strains such as disease resistance, drought resistance, moisture resistance and the like and the seed quality (comparison among strains) of the seeds obtained by mixing and harvesting the F7 generation in the step (4) in the field planting period, and obtaining the strains with better comprehensive properties and limited capsules to carry out a comparison test, wherein the comparison test method comprises the following steps: the test plot is 3 m long and 2m wide, and is designed in random block mode, the three times of the test plots are repeated, the planting row spacing is 40cm, 1-2 pairs of true leaf period seedlings are planted, 3-4 pairs of true leaf period seedlings are set, the plant spacing is 10cm, and other field management is performed as usual. The main traits are shown in Table 1. The obtained improved strains SR06, SR09 and SR24 (self-naming) with limited growth habit and plutella xylostella trait have the average yield increased by 40.05%, 41.22% and 40.91% respectively compared with the parents in two years, and the high-yield improvement target of the limited growth habit sesame is realized.
TABLE 1 comparison of traits in NFS23 and improved progeny lines
TABLE 2 sesame seed resources
Example 2
The materials used in this example are 107 sesame germplasm resources and a limited growth habit sesame line "ST 04" (ST04 provided by crop germplasm resources center of Jiangxi province and crop research institute of Jiangxi province academy of agricultural sciences and named by number; ST04 obtained by hybrid breeding of dt45 and Zhongzhi No. 11 stored in middle-stage bank of oil crop germplasm resources of the institute of oil crop of Chinese academy of agricultural sciences) in example 1, and the specific operation steps are as follows:
(1) Artificial hybridization was carried out using the sesame seeds of the multi-capsule type "RX 786" and the sesame seeds of limited growth habit "ST 04" obtained in steps (1) and (2) of example 1 as parents. The specific method comprises the following steps: and (3) manually emasculating one of the parents in the evening of the first day, and pollinating the pistil of the artificially emasculated parent by taking pollen of a flower which is opened by the other parent in the morning of the next day.
(2) The specific operation of planting and selecting each generation of the filial generation is the same as that of the step (4) in the example 1.
Identifying the stress resistances of various strains such as disease resistance, drought resistance, moisture resistance and the like and the seed quality (comparison among strains) of the seeds obtained by the mixed harvest of the F7 generation obtained in the step (2) in the field planting period, and obtaining the strains with better comprehensive properties and limited capsules to carry out a comparison test, wherein the comparison test method comprises the following steps: the test cell is 3 meters long and 2 meters wide, random block design is carried out, three times of repetition are carried out, the planting row spacing is 40cm, 1-2 pairs of seedlings in the true leaf stage, 3-4 pairs of seedlings in the true leaf stage are set, the plant spacing is 10cm, other field management is as usual, the two-year average yield of 3 improved strains TR19, TR28 and TR39 (self-naming) with limited growth habit and multi-capsule character obtained as shown in the table 3 is respectively increased by 36.62%, 39.10% and 37.46% compared with that of the parent, and the detailed table 3 shows that the two-year average yield of the improved strains TR19, TR28 and TR39 is higher than that of the parent. Realizes the goal of high yield improvement of the sesame with limited growth habit.
TABLE 3 comparison of traits in ST04 with improved progeny lines
Experimental example 1
The experimental example investigates the influence of different planting densities on the character occurrence rate of each axillary polychaellus of the RX786 sesame, wherein the character occurrence rate of each axillary polychaes of the RX786 plant (the character occurrence rate of a polychaes plant) refers to the percentage of the number of sesame plants, in which 5-7 capsules appear in each axillary epipolar position, of more than or equal to 50% of the middle nodes, to the total number of sesame plants in a cell.
The test method comprises the following steps: the cells were set according to the row spacing in table 3, with a cell width of 2m, 10 rows were planted in each cell, and the blocks were randomly organized and repeated three times. Seedlings are grown in 1-2 pairs of true leaf stages, and seedlings are grown in 3-4 pairs of true leaf stages.
The sesame germplasm resource of RX786 is implemented according to the steps (1) and (2) in the example 1, wherein the row spacing and the seedling spacing after the final singling in the step (1) are shown in the following table 4, and the statistical occurrence rate of the behavior of the oxpockmula of RX786 plants per leaf is shown in the following table 4.
TABLE 4 RX786 plants axillary Populus capsularis trait incidence results
As is clear from the above Table 4, the occurrence of the per-capsule character of the triple-capsule type sesame can be remarkably improved by controlling the row pitch/cm and the plant pitch/cm.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (5)
1. A high-yield breeding method of limited growth habit sesame is characterized by comprising the following steps:
s1, sowing the three capsule type sesame germplasm resources according to the row spacing of more than or equal to 40cm, and thinning in the true leaf stage at 1-2 times, wherein the seedling spacing is 3-5 cm; performing final singling at the true leaf stage at 3-4, wherein the seedling spacing is more than or equal to 30 cm;
s2, observing and recording the flowering condition of the sesame plants in the step S1 after the sesame plants enter the full-bloom stage, and marking the germplasm resources with the axillary flowering number of each leaf being more than 3; investigating and recording the capsule condition after final flowering, and marking the germplasm resources with the number of capsules per axilla being more than 3; after the plants are mature, germplasm resource markers with the axillary flowering number and the capsule number larger than 3 are subjected to seed test analysis, sesame plants with S capsules growing in each axillary are selected from more than or equal to 50% of nodes in the middle of the plants, and S is more than or equal to 5 and less than or equal to 7, so that the sesame germplasm with multiple capsules is obtained;
s3, carrying out artificial hybridization by taking the multi-capsule sesame germplasm and the sesame with limited growth habit obtained in the step S2 as parents, and harvesting F1 generation hybrid seeds;
s4, planting F1 generation hybrid seeds to obtain F1 plants, and after the F1 plants bloom, carrying out mixed harvesting on plants with the number of axillary capsules of each node more than or equal to 5 and more than or equal to 50% of the nodes in the middle of the F1 plants to obtain F2 generation seeds; the hybrid seeds are planted in a sparse planting mode during planting, wherein the sparse planting mode is as follows: sowing the hybrid seeds according to the row spacing of more than or equal to 40cm, thinning the seedlings at the true leaf stage by 1-2 pairs, and keeping the seedling spacing between 3-5 cm; performing final singling at the true leaf stage at 3-4, wherein the seedling spacing is more than or equal to 30 cm;
S5, planting F2 generation seeds to obtain an F2 separation population, after the plants of the F2 separation population bloom and capsula, selecting individual plants which have the number of capsules per axillary capsule of more than or equal to 50% of the nodes in the middle of the plants and show limited growth habit, marking and numbering the individual plants, and harvesting the individual plants after maturation to obtain F3 generation seeds; then planting the seeds of the F3-F6 generation according to the method, selecting a plant line which has the characteristics that the number of capsules per leaf in the section which is more than or equal to 50 percent of the sections in the middle of the plant and the limited growth habit are simultaneously numbered, and harvesting the plants according to the single plant after the plants are mature; and finally planting the generation seeds above F7 and harvesting in a mixing way.
2. The high yield breeding method of sesame having a limited growth habit according to claim 1, wherein the row spacing is 40-60cm and the row spacing after final singling is 30-40cm in step S1.
3. A high-yield breeding method of limited growth habit sesame as claimed in claim 1, wherein said hybrid seeds are planted with a row spacing of 40-60cm and a seedling spacing of 30-40cm after final singling.
4. A high-yield breeding method of sesame with limited growth habit according to claim 1, 2 or 3, characterized in that the number of segregating population plants per generation obtained by artificial hybridization is more than or equal to 30.
5. A high yield breeding method of sesame having a limited growth habit according to claim 1, 2 or 3, wherein the artificial hybridization is: the artificial castration is carried out on one of the parents in the evening of the previous day, and then pollen of the flower which is opened by the other parent is taken in the morning of the next day to pollinate the pistil of the parent after the artificial castration.
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| Inheritance of number of capsules per leaf axil and hairiness on stem, leaf and capsule of sesame (Sesamum indicum L.);Engin Yol等;《Australian journal of crop science》;20111231;第78-81页 * |
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