CN103053410B - Breeding method of two-line selenium-enriched three-color formula fragrant rice hybrid rice variety - Google Patents
Breeding method of two-line selenium-enriched three-color formula fragrant rice hybrid rice variety Download PDFInfo
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 105
- 235000009566 rice Nutrition 0.000 title claims abstract description 104
- 239000011669 selenium Substances 0.000 title claims abstract description 73
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 66
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 66
- 241000209094 Oryza Species 0.000 title claims abstract 30
- 238000009395 breeding Methods 0.000 title abstract description 8
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 79
- 239000003205 fragrance Substances 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 9
- 241000196324 Embryophyta Species 0.000 claims description 25
- 244000236458 Panicum colonum Species 0.000 claims description 12
- 235000015225 Panicum colonum Nutrition 0.000 claims description 12
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 235000021329 brown rice Nutrition 0.000 claims description 8
- 235000013339 cereals Nutrition 0.000 claims description 8
- OIQPTROHQCGFEF-UHFFFAOYSA-L chembl1371409 Chemical compound [Na+].[Na+].OC1=CC=C2C=C(S([O-])(=O)=O)C=CC2=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 OIQPTROHQCGFEF-UHFFFAOYSA-L 0.000 claims description 8
- 238000009396 hybridization Methods 0.000 claims description 8
- 230000005070 ripening Effects 0.000 claims description 8
- 241000231392 Gymnosiphon Species 0.000 claims description 5
- 125000003118 aryl group Chemical group 0.000 claims description 5
- 238000003306 harvesting Methods 0.000 claims description 5
- 230000035558 fertility Effects 0.000 claims description 4
- 230000036512 infertility Effects 0.000 claims description 4
- 230000008774 maternal effect Effects 0.000 claims description 4
- 230000008775 paternal effect Effects 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 230000008929 regeneration Effects 0.000 claims description 4
- 238000011069 regeneration method Methods 0.000 claims description 4
- 238000004513 sizing Methods 0.000 claims description 4
- 238000009331 sowing Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 235000016709 nutrition Nutrition 0.000 abstract description 7
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 abstract description 6
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 239000003086 colorant Substances 0.000 abstract description 3
- 229960003692 gamma aminobutyric acid Drugs 0.000 abstract description 3
- 230000036541 health Effects 0.000 abstract description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract description 2
- 239000011707 mineral Substances 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 description 75
- 230000035764 nutrition Effects 0.000 description 5
- 244000062793 Sorghum vulgare Species 0.000 description 4
- 235000019713 millet Nutrition 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
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- 230000001502 supplementing effect Effects 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 208000030507 AIDS Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000002177 Cataract Diseases 0.000 description 1
- 241000371652 Curvularia clavata Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000007189 Oryza longistaminata Nutrition 0.000 description 1
- 208000017442 Retinal disease Diseases 0.000 description 1
- 206010038923 Retinopathy Diseases 0.000 description 1
- 206010039921 Selenium deficiency Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
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- 235000013599 spices Nutrition 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
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- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
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Abstract
The invention discloses a breeding method of a two-line selenium-enriched three-color formula fragrant rice hybrid rice variety, and belongs to the breeding technical field of special rice. According to the method provided by the invention, a selenium-enriched green rice variety and a selenium-enriched giant embryo rice variety are hybridized to cultivate fragrant green giant embryo rice variety through selection of characters of multiple generations. A fragrant two-line sterile line and yellow rice are hybridized to cultivate a fragrant yellow rice two-line sterile line through the selection of the characters of the multiple generations. The fragrant yellow rice two-line sterile line and the selenium-enriched fragrant green giant embryo rice are hybridized to cultivate hybrid F1-generation seeds, namely, fragrant yellow and green giant embryo hybrid F1. After the F1 is planted, selenium-enriched fragrant rice with different colors and types can be produced by using harvested rice after being subjected to roughness removal. According to the breeding method, the two-line selenium-enriched three-color formula fragrant rice hybrid rice variety is bred, the production method is simple, high in efficiency and good in effect. The produced functional selenium-enriched three-color formula fragrant rice can integrate fragrance, color, taste and nutritional curative effects. Nutritional ingredients such as selenium element, gamma-aminobutyric acid and various mineral substances can be effectively supplemented for a user after being eaten for a long time so as to promote the human health.
Description
Technical field
It is the selection of rich selenium three look formula scented rice Hybrid Rice Varieties that the present invention is specifically related to a kind of two, belongs to special rice breeding field.
Background technology
In recent years, along with the aggravation of environmental pollution, the remarkable change of people life style and meals mode, mineral element nutrient imbalance has become the key factor that affects human health.Selenium is the human body necessary trace element that grows; to disease preventing and treating with prevent old and feeble significant; be described as " king of anti-cancer and cancer-preventing " by scientist; the various diseases such as carninomatosis, cardiopathy, diabetes, retinopathy, cataract, cardiovascular disease are had to unique prevention and result for the treatment of, and appropriate selenium is taken in the effect that can also promote growth, protection vision, delay senility and have opposing AIDS.There is selenium deficiency in various degree in the population of China 2/3, and the side effect of medicine benefit selenium is large, expensive, is difficult to effectively supplement the selemium nutrition of human body.Paddy rice selenium content height is closely related with human body selemium nutrition situation, and at present selenium-rich rice to produce maximum be the content that enriches external source selenium and improve selenium in rice, but this spraying method is produced not only expensive effort of rich selenium rice and not environmental protection.Utilize the enrichment effect of paddy rice to selenium itself, the selenium-rich rice kind that seed selection rice Se content is high is the most effective way.The seed coat of green rice is green, and it is nutritious, and particularly selenium content is high, and selenium content is up to 23.2mg/100g.In addition, green rice also contains trace element and the vitamin of a large amount of needed by human, and people often eat green rice can reach benefit selenium health care object.Glutinous millet is to produce yellow pericarp by having yellow anthocyan in pericarp, and anthocyan is described as " the special effect element of life ".The various amino acid contents of Characters of Giant Embryo Rice are high, and are rich in functional component γ-aminobutyric acid, selenium and various mineral matter.Scented rice kind has special fragrant, makes rice more nice good to eat.If these several rice formulas are edible,, to supplementing human nutrition, ensure healthy benefiting.Current market formula rice preparation method, by dissimilar rice separate plant development again mechanical mixture form, be all mainly that black rice, glutinous rice, red rice are mixed in proportion, this kind of method operation is loaded down with trivial details, and production cost is higher.There is no at present report both at home and abroad and once plant kind and the breeding method that just can produce the formula rice that green rice, glutinous millet, rice and giant embryo, the fragrance etc. of rich selenium combines.
Summary of the invention
The object of the present invention is to provide a kind of two to be the selection of rich selenium three look formula scented rice Hybrid Rice Varieties, the method is utilized Genetic and breeding in rice method, efficiently the hybrid rice seed of the fragrant rice of the rich selenium of seed selection different colours.Reach as long as plant a kind of hybrid rice seed, the paddy of its harvest can contain the object of the fragrant rice of rich selenium of number of different types and color simultaneously, not only can produce the functional fragrant rice of the high nutrition of rich selenium, and production process is simple, and production cost is low.
Content of the present invention is two to be the selection of rich selenium three looks formula scented rice Hybrid Rice Varieties, the method seed selection efficiently two is rich selenium three look aromatic rices, and this kind plantation can be produced the little embryo of yellow of rich selenium, yellow giant embryo, green little embryo, green giant embryo, white little embryo, white giant embryo rice simultaneously.Its principle is: the rich selenium characteristic of paddy rice is relevant to the hereditary capacity of paddy rice, the proterties such as fragrance proterties, yellow seed coat, green seed coat and the giant embryo of paddy rice are controlled by gene, it is in the male parent or female parent of paddy rice that these genes are imported respectively to two, the paddy (F that the first generation of hybrid seed of its preparation is gathered in the crops after plantation on producing
2) produce the fragrant rice of rich selenium dissimilar and color because controlling the separation of these character genes.
Technical scheme of the present invention is as follows:
Two is a selection for rich selenium three look formula scented rice Hybrid Rice Varieties, comprises the following steps:
(1) with the Characters of Giant Embryo Rice inter breed crossing of green aromatic rice and the rich selenium of rich selenium, plantation hybridization F
1generation, F
2-F
5go out rough selection and have simultaneously the brown rice of green seed coat, giant embryo and fragrance proterties for pedigree, and to F
2generation-F
5select for pedigree economical character, yield traits, Se content, selection criteria is that plant cane is sturdy, tiller moderate (10-13/clump), grain number per spike is many, ripening rate is high and selenium content is high individual plant, be bred as the odor type green rice Characters of Giant Embryo Rice " fragrant green Characters of Giant Embryo Rice " of rich selenium, containing the gene of controlling fragrance, green seed coat and giant embryo proterties;
(2) select odor type two-line sterile line to do maternal, make paternal hybrid with glutinous millet rice, plantation hybridization F
1generation, F
2generation-F
6in generation, is pressed pedigree method plantation, plant bore seeds and all went out the brown rice plantation that rough selection has yellow seed coat and fragrance proterties simultaneously per generation, per generation therefrom selects that plant cane is sturdy again, 100 of the sterile plants of tiller moderate (10-12/clump), big panicle many grains per panicle, cut 23 ℃ of regeneration of shift-in climatic cabinate after pocket, select the individual plant sowing that pollen loose powder is good, ripening rate is high, Se content is high, F
6in generation, selects population characteristics to stablize, do not separate, the strain that sterility is stable and fertility is changed, and select wherein plant cane individual plant sizing sturdy, tiller moderate (10-12/clump), big panicle many grains per panicle to be bred as odor type glutinous millet rice two-line sterile line " fragrant yellow S ";
(3) produce hybrid F with odor type glutinous millet rice two-line sterile line " fragrant yellow S " and odor type green rice Characters of Giant Embryo Rice " the fragrant green Characters of Giant Embryo Rice " hybridization of rich selenium
1for seed " fragrant yellowish green giant embryo F
1", it is simultaneously containing controlling fragrance (isozygotying), yellow race's skin (heterozygosis), green seed coat (heterozygosis) and giant embryo (heterozygosis) gene;
(4) hybrid F
1for seed " fragrant yellowish green giant embryo F
1" as producing with planting.
Described hybrid F
1for seed " fragrant yellowish green giant embryo F
1" paddy of harvesting goes out after rough to produce following 6 kinds of fragrant rice of rich selenium dissimilar and color after plantation: yellow little embryo, yellow giant embryo, green little embryo, green giant embryo, white little embryo, white giant embryo.
Beneficial effect of the present invention:
Effect of the present invention is that the gene pyramiding of the proterties such as the rich selenium of handle control simultaneously, fragrance, green seed coat, yellow race's skin, giant embryo is in hybrid F
1generation, this hybrid F of plantation in production
1the separation of the paddy Yin Jiyin that Dai Houqi gathers in the crops and restructuring and produce the fragrant rice of rich selenium of different colours and embryo size combinations.Advantage of the present invention is the double-line hybrid rice that can directly produce " rich selenium three look scented rices " by breeding method seed selection, plants this seed and can produce selenium-rich function type three look scented rices.Production method is simple, efficiency is high, profitable, the rice of production is rich in the various nutritive elements such as selenium, γ-aminobutyric acid, to supplementing the selemium nutrition of human body, ensures healthy benefiting.
embodiment
Embodiment 1:
(1) the green aromatic rice of rich selenium " precious No. one of medium green " is hybridized with the Characters of Giant Embryo Rice kind " gewx1 " of rich selenium, F
2-F
5in generation, goes out rough selection and has simultaneously the brown rice of green seed coat, giant embryo and fragrance proterties; F
2generation-F
5select for pedigree that plant cane is sturdy, the individual plants moderate, that grain number per spike is many, ripening rate is high and selenium content is high of tillering, be bred as the green meter of Characters of Giant Embryo Rice of odor type " No. 1, fragrant green Characters of Giant Embryo Rice " of rich selenium;
(2) select odor type two-line sterile line " bright fragrant 10S " to do maternal, make paternal hybrid, F with glutinous millet rice " glutinous millet is glutinous "
2generation-F
6plant for pedigree method, plant bore seeds and all went out the brown rice plantation that rough selection has yellow seed coat and fragrance proterties simultaneously per generation, per generation more therefrom selection traits show shift-in climatic cabinate 23 degree regeneration after the complete sterile individual plant of 100 → pollen of excellent sterile plant microexamination → select → cut pocket → select pollen loose powder good, the individual plant sowing that ripening rate is high, Se content is high.F
6generation selection population characteristics is stable not to be separated, and the strain that sterility is stable and fertility is changed also selects the wherein individual plant sizing of excellence to be bred as odor type glutinous millet rice two-line sterile line " No. 1, fragrant yellow S ";
(3) produce hybrid F with odor type glutinous millet rice two-line sterile line " No. 1, fragrant yellow S " and odor type green rice Characters of Giant Embryo Rice " No. 1, the fragrant green Characters of Giant Embryo Rice " hybridization of rich selenium
1for seed " No. 1, fragrant yellowish green giant embryo ", it is simultaneously containing controlling fragrance (isozygotying), yellow race's skin (heterozygosis), green seed coat (heterozygosis) and giant embryo (heterozygosis) gene;
(4) hybrid F
1for seed " No. 1, fragrant yellowish green giant embryo " as produce with kind, after its plantation, paddy of harvesting goes out can produce after rough the fragrant rice of rich selenium of following several types: yellow little embryo, yellow giant embryo, green little embryo, green giant embryo, white little embryo, white giant embryo.
Embodiment 2:
(1) the green aromatic rice of rich selenium " precious No. one of medium green " is hybridized with the Characters of Giant Embryo Rice kind " ge3 " (document: Zhang Yanhua, University Of Agriculture and Forestry In Fujian's master thesis,, 35 pages in 2010) of rich selenium, F
2-F
5in generation, goes out rough selection and has simultaneously the brown rice of green seed coat, giant embryo and fragrance proterties; F
2generation-F
5select for pedigree that plant cane is sturdy, the individual plants moderate, that grain number per spike is many, ripening rate is high and selenium content is high of tillering, be bred as the green meter of Characters of Giant Embryo Rice of odor type " No. 2, fragrant green Characters of Giant Embryo Rice " of rich selenium;
(2) select odor type two-line sterile line " fine jade perfume (or spice)-1S " to do maternal, make paternal hybrid, F with glutinous millet rice " glutinous millet is glutinous "
2generation-F
6plant for pedigree method, plant bore seeds and all went out the brown rice plantation that rough selection has yellow seed coat and fragrance proterties simultaneously per generation, per generation more therefrom selection traits show shift-in climatic cabinate 23 degree regeneration after the complete sterile individual plant of 100 → pollen of excellent sterile plant microexamination → select → cut pocket → select pollen loose powder good, the individual plant sowing that ripening rate is high, Se content is high.F
6generation selection population characteristics is stable not to be separated, and the strain that sterility is stable and fertility is changed also selects the wherein individual plant sizing of excellence to be bred as odor type glutinous millet rice two-line sterile line " No. 2, fragrant yellow S ";
(3) produce hybrid F with odor type glutinous millet rice two-line sterile line " No. 2, fragrant yellow S " and rich odor type green rice Characters of Giant Embryo Rice " No. 2, fragrant green Characters of Giant Embryo Rice " hybridization
1for seed " No. 2, fragrant yellowish green giant embryo ", it is simultaneously containing controlling fragrance (isozygotying), yellow race's skin (heterozygosis), green seed coat (heterozygosis) and giant embryo (heterozygosis) gene;
(4) hybrid F
1for seed " No. 2, fragrant yellowish green giant embryo " as produce with kind, after its plantation, paddy of harvesting goes out can produce after rough the fragrant rice of rich selenium of following several types: yellow little embryo, yellow giant embryo, green little embryo, green giant embryo, white little embryo, white giant embryo.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. two is a selection for rich selenium three look formula scented rice Hybrid Rice Varieties, it is characterized in that: described selection comprises the following steps:
(1) with the Characters of Giant Embryo Rice inter breed crossing of green aromatic rice and the rich selenium of rich selenium, plantation hybridization F
1generation, F
2-F
5go out rough selection and have simultaneously the brown rice of green seed coat, giant embryo and fragrance proterties for pedigree, and to F
2generation-F
5select for pedigree economical character, yield traits, Se content, selection criteria is that plant cane is sturdy, the individual plants moderate, that grain number per spike is many, ripening rate is high and selenium content is high of tillering, be bred as the odor type green rice Characters of Giant Embryo Rice " fragrant green Characters of Giant Embryo Rice " of rich selenium, containing the gene of controlling fragrance, green seed coat and giant embryo proterties;
(2) select odor type two-line sterile line to do maternal, make paternal hybrid with glutinous millet rice, plantation hybridization F
1generation, F
2generation-F
6in generation, is pressed pedigree method plantation, plant bore seeds and all went out the brown rice plantation that rough selection has yellow seed coat and fragrance proterties simultaneously per generation, per generation therefrom selects that plant cane is sturdy again, 100 of the sterile plants of moderate, the big panicle many grains per panicle of tillering, cut 23 ℃ of regeneration of shift-in climatic cabinate after pocket, select the individual plant sowing that pollen loose powder is good, ripening rate is high, Se content is high, F
6generation select population characteristics stable, do not separate the strain that sterility is stable and fertility is changed, and select wherein that plant cane is sturdy, the individual plant sizing moderate, big panicle many grains per panicle of tillering is bred as odor type glutinous millet rice two-line sterile line " fragrant yellow S ";
(3) produce hybrid F with odor type glutinous millet rice two-line sterile line " fragrant yellow S " and odor type green rice Characters of Giant Embryo Rice " the fragrant green Characters of Giant Embryo Rice " hybridization of rich selenium
1for seed " fragrant yellowish green giant embryo F
1", containing controlling, fragrance isozygotys simultaneously for it, yellow race's skin heterozygosis, green seed coat heterozygosis and giant embryo heterozygous genes;
(4) hybrid F
1for seed " fragrant yellowish green giant embryo F
1" as producing with planting.
2. according to claim 1 a kind of two is the selection of rich selenium three look formula scented rice Hybrid Rice Varieties, it is characterized in that: described hybrid F
1for seed " fragrant yellowish green giant embryo F
1" paddy of harvesting goes out after rough to produce following 6 kinds of fragrant rice of rich selenium dissimilar and color after plantation: yellow little embryo, yellow giant embryo, green little embryo, green giant embryo, white little embryo, white giant embryo.
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| CN107027620B (en) * | 2016-10-27 | 2020-08-25 | 江西省农业科学院农产品质量安全与标准研究所 | Breeding method of plant rich in selenium and gamma-aminobutyric acid simultaneously |
| CN107951014A (en) * | 2017-12-26 | 2018-04-24 | 王静婵 | Selenium-rich Herba Andrographitis health care nutrition powder |
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| 功能稻育种进展与产品开发;何琴等;《福建稻麦科技》;20041231;第22卷(第4期);第41-44页 * |
| 巨胚水稻W025 在贵州省的适应性及其品质分析;张玉珊等;《贵州农业科学》;20111231;第39卷(第5期);第4-7页 * |
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Inventor after: Bie Chao Inventor before: Zhang Qingqi Inventor before: Zheng Zhi Inventor before: Huang Ronghua Inventor before: Li Meide Inventor before: Yang Fan |
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Effective date of registration: 20170808 Address after: 710016 Shaanxi province Xi'an Weiyang road Fengcheng two times square B block 1616 Patentee after: Bie Chao Address before: Cangshan District of Fuzhou City, Fujian province 350002 to build a new town, Jinshan District Patentee before: FUJIAN AGRICULTURE AND FORESTRY University |
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Effective date of registration: 20180508 Address after: 415000 eight groups of Guanyin Bridge village, Taoyuan Town, Taoyuan, Changde, Hunan Patentee after: CHANGDE LONGFENG RICE Co.,Ltd. Address before: 710016 B 1616, Tiandi Times Plaza, Fengcheng two road, Weiyang District, Xi'an, Shaanxi. Patentee before: Bie Chao |
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Granted publication date: 20140528 |