CN104823839B - Breeding method of anthocyanin-rich black rice thermo-sensitive two-line sterile line - Google Patents
Breeding method of anthocyanin-rich black rice thermo-sensitive two-line sterile line Download PDFInfo
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Abstract
The invention discloses a breeding method of an anthocyanin-rich black rice thermo-sensitive two-line sterile line and belongs to the technical field of special rice breeding. The method comprises the following steps: taking black rice with the brown rice anthocyanin color value which is more than 20 as a male parent, and taking a thermo-sensitive two-line sterile line as a female parent, and carrying out artificial hybridization; selecting single plants which has the brown rice anthocyanin color value more than 20, stable fertility, low critical temperature, long stable sterile period, thick and strong plant stalks, strong tillering, up-right leaves, small leaf angles and many spike grains in each generation from the generation F2; and finally, breeding to form the anthocyanin-rich black rice thermo-sensitive two-line sterile line. By virtue of the method, the foundation is laid for the assembling of hybrid black rice high-quality seeds which are rich in anthocyanin and have stable yield and high yield. The bred sterile line is used for breeding hybrid black rice varieties so that the bred hybrid black rice has good plant shape, high yield and stable yield; and the brown rice has bright color and luster, is dull black and is rich in anthocyanin, and has advantages of hybrids.
Description
Technical field
The invention belongs to Special Rices breeding technical field is and in particular to a kind of black rice temperature sensitive two rich in anthocyanin is not
Educate the selection being.
Background technology
With the continuous improvement of our people's living standard, the requirement to rice alimentary health-care function for the people improves constantly.
Rice breeding also switchs to yield and quality from simple attention yield and takes into account, and the orientation of the special Functional Components In Rice of pay attention to day by day
Breeding.
, also known as black rice, nutrition, medical value are all very high, are just acknowledged as excellent tonic product since ancient times, are also the successive dynasties for fructus zizaniae caduciflorae
The tonic that imperial palace is enjoyed, is described as " tribute rice ", have " rice of enriching blood ", " long-lived rice ", " medicine rice ", the laudatory title of " angle's rice ",
China's cultivation history of existing more than 1,500 year.Described in the pharmaceutical texts in successive dynasties, fructus zizaniae caduciflorae energy " nourishing YIN and invigorating YANG, in appetizing benefit,
Strengthening spleen and warming liver, improving eyesight is invigorated blood circulation, and cunning is puckery to mend clearly." measure through science, in fructus zizaniae caduciflorae, protein content is 8.5～8.8%, fat content
For 2.702%, higher more than 2 times than general rice, in black rice b, c vitamin, essential amino acid and ferrum,
Zinc, selenium and other trace elements, all high than general rice, some even general rice are unexistent.Additionally, fructus zizaniae caduciflorae also contains very
Important active component anthocyanin.Black Rice Anthocyanin-rich belongs to flavone compound, is with glucosides by anthocyanidin with various sugar
The glucosides that key combines to form.Research finds, anthocyanin class material has antioxidation, antiinflammatory, blood fat reducing and suppression tumor and generates
Etc. physiological function.
Black rice nourishing health value rich in anthocyanin is high, but black rice yields poorly, different cultivars anthocyanin content difference
Very big, greatly limit the exploitation further of black rice, in order to improve yield and the anthocyanin character of black rice varieties, must
Breed improvement must be carried out.
It is selenium-rich trichroism formula scented rice hybrid paddy rice that the patent of invention of Publication No. cn 103053410 a discloses a kind of two
The selection of kind, the hybrid f that the sterile line of its selection-breeding is joined1For seed plantation after harvesting Oryza glutinosa go out rough after be yellow,
Green, white, not black, it is not rich in anthocyanin yet.The patent of invention of Publication No. cn 102715076 a discloses one
Kind of five-color rice and its selection, the brown rice color of the five-color rice of its selection-breeding is black, sepia, redness, yellow, white
Color, but the black rice varieties of its selection-breeding are conventional Rice, and it is not rich in anthocyanin.The invention of Publication No. cn 102630557 a is special
Profit discloses a kind of selection of functional formula rice variety, and the formula rice kind Oryza glutinosa of its selection-breeding grinds the rice can be simultaneously
Containing fructus zizaniae caduciflorae, purple rice, red rice, Oryza glutinosa, 5 kinds of functional rices of giant embryo rice, but the kind of selection-breeding is also conventional rice variety, and is not rich in
Anthocyanin.The patent of invention of Publication No. cn 102524085 a discloses a kind of giant embryo Black Rice and its selection, is logical
Cross mutation Black Rice and carry out selection-breeding giant embryo Black Rice, but the kind of selection-breeding is also conventional rice variety, is not also rich in anthocyanin.Publication number
Patent of invention for cn 102630557 a discloses a kind of selection having and producing colorful rice function rice, but it is by choosing
Educate fructus zizaniae caduciflorae CMS line to obtain fructus zizaniae caduciflorae hybrid paddy rice, rather than selection-breeding fructus zizaniae caduciflorae two-line sterile line, it is not rich in anthocyanin yet.
Content of the invention
Based on above-mentioned background, it is an object of the invention to provide a kind of rich in the temperature sensitive two-line sterile line of anthocyanin black rice
Selection, is to lay a good foundation rich in anthocyanin, assembling of stable and high yields fructus zizaniae caduciflorae hybrid paddy rice breeding.
For achieving the above object, the present invention adopts the following technical scheme that
The invention provides a kind of selection of the temperature sensitive two-line sterile line of black rice rich in anthocyanin, male parent is selected rough
Rice anthocyanin colour value reaches more than 20 black rice, and female parent is selected temperature sensitive two-line sterile line, carried out artificial hybridization, from f2In generation, starts every
In generation, all carries out the selection of three reselection procedure anthocyanin contents, the selection of fertility, the selection of economical character, and final selection-breeding becomes to be rich in
The temperature sensitive two-line sterile line of black rice of anthocyanin, the temperature sensitive two-line sterile line anthocyanin content of described black rice, stable fertility, faces
Boundary's temperature is low and steady sterile period length, plant stalk are sturdy, tiller is strong, upright blade, leaf angle are little, grain number per spike is many.
Further, described selection comprises the following steps:
1) more than 20 black rice is reached as male parent with brown rice anthocyanin colour value, with temperature sensitive two-line sterile line as female parent, carry out
Artificial hybridization, plantation hybridization f1Generation；
2) plant f2Generation, f2For more than 2000 plants of colony, Oryza sativa L. is from the formation stage of pistil and stamen to pollen mother cell meiosis
I.e. the temperature of the 4-6 phase of ear differentiation, not above 23.5 DEG C, carries out fertility screening, and selection plant stalk is sturdy, tiller is strong, leaf
Piece is upright, leaf angle is little, the sterile line individual plant more than grain number per spike, carries out anthocyanin content mensure to the brown rice of the individual plant chosen seeds, choosing
Select the individual plant of anthocyanin content；
3) f3~f6From generation to generation, each from generation to generation to upper one from generation to generation selected by individual plant press strain and plant, Oryza sativa L. from the formation stage of pistil and stamen to
Pollen mother cell meiosis be the temperature of the 4-6 phase of ear differentiation not above 23.5 DEG C, carry out fertility screening, select to plant
Stalk is sturdy for strain, tiller is strong, upright blade, leaf angle are little, the sterile line individual plant more than grain number per spike, and the brown rice of the individual plant chosen seeds is entered
Row anthocyanin content measures, and selects the individual plant of anthocyanin colour value content；
4) f7The individual plant of the anthocyanin content that generation is chosen to step 3) is planted by strain, selects critical temperature low and steady
The stable strain of fixed sterile phase length；
5) in growth cabinet, the sterile changeover temperature of chosen strain is identified, select sterile line origin temp
≤ 22.5 DEG C of strain, that is, be bred as the temperature sensitive two-line sterile line of black rice rich in anthocyanin.
Preferably, described male parent selects black rice " nantan84 ".
Preferably, described female parent selects temperature sensitive two-line sterile line 272s.
The invention provides a kind of selection rich in the temperature sensitive two-line sterile line of anthocyanin black rice, wherein, the present invention
The selection of parent is the key of the present invention.It is an advantage of the current invention that the fructus zizaniae caduciflorae two-line sterile line anthocyanin colour value of selection-breeding can reach 20
Above, stable fertility, critical temperature are low and steady sterile period length, plant stalk are sturdy, tiller is strong, upright blade, leaf angle are little, fringe
Grain number is many；Institute's selection-breeding be applied to fructus zizaniae caduciflorae Hybrid Rice Varieties selection-breeding rich in anthocyanin fructus zizaniae caduciflorae sterile line, the fructus zizaniae caduciflorae of institute's selection-breeding can be made
Hybrid paddy rice plant forms are good, high yield, stable yields, its brown rice bright in color, pitch-dark, be rich in anthocyanin, and there is heterobeltiosis.
Specific embodiment
In order that content of the present invention easily facilitates understanding, with reference to specific embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
Embodiment 1:
1) 20.60 black rice " nantan84 " is reached as male parent with anthocyanin colour value, to contribute to the accumulation of hybrid anthocyanin
Temperature sensitive two-line sterile line 272s be female parent, carry out artificial hybridization, plantation hybridization f1Generation；
2) plant f2Generation, f2For more than 2000 plants of colony, planting site selects Oryza sativa L. from the formation stage of pistil and stamen to pollen mother cell
Reduction division phase (i.e. the 4-6 phase of ear differentiation) temperature can be maintained at less than 23.5 DEG C of place, carries out fertility screening, selects to plant
Stalk is sturdy for strain, tiller is strong, upright blade, leaf angle are little, the sterile line individual plant more than grain number per spike, and the brown rice of the individual plant chosen seeds is entered
Row anthocyanin content measures, and selects anthocyanin colour value to reach more than 20 individual plant；
3) f3~f6From generation to generation, the individual plant that each reaches more than 20 from generation to generation to the anthocyanin colour value chosen is planted by strain, each
42 plants of strain, planting site selects Oryza sativa L. from the formation stage of pistil and stamen to pollen mother cell meiosis (the i.e. 4-6 of ear differentiation
Phase) temperature can be maintained at less than 23.5 DEG C of place, carry out fertility screening, select that plant stalk is sturdy, tiller is strong, blade is straight
Vertical, leaf angle is little, the sterile line individual plant more than grain number per spike, carries out anthocyanin content mensure to the brown rice of the individual plant chosen seeds, selects flower
Color glycosides colour value reaches more than 20 individual plant；
4) f7The individual plant that generation reaches more than 20 to the anthocyanin colour value chosen was observed by the anniversary that strain plantation carries out system,
Select that critical temperature is low and the stable strain of steady sterile period length.
5) in growth cabinet, the sterile changeover temperature of chosen strain is identified, select sterile line origin temp
≤ 22.5 DEG C of strain d38s.
22.5 DEG C of the black rice two-line sterile line d38s critical temperature of institute's selection-breeding, stable fertility and steady sterile period is long, plant
Stalk is sturdy for strain, tiller is strong, upright blade, leaf angle are little, and its brown rice anthocyanin colour value is up to 24.29.
Embodiment 2:
1) 20.60 black rice " nantan84 " is reached as male parent with anthocyanin colour value, wide with light temperature sensitive two-line sterile line product
Accounting for 63s is female parent, carries out artificial hybridization, plantation hybridization f1Generation；
2) plant f2Generation, f2For more than 2000 plants of colony, planting site selects Oryza sativa L. from the formation stage of pistil and stamen to pollen mother cell
Reduction division phase (i.e. the 4-6 phase of ear differentiation) temperature can be maintained at less than 23.5 DEG C of place, carries out fertility screening, selects to plant
Stalk is sturdy for strain, tiller is strong, upright blade, leaf angle are little, the sterile line individual plant more than grain number per spike, and the brown rice of the individual plant chosen seeds is entered
Row anthocyanin content measures, and selects the individual plant of anthocyanin content, but selected individual plant anthocyanin colour value is below 20；
3) f3~f6From generation to generation, each is planted by strain to the individual plant chosen from generation to generation, 42 plants of each strain, and planting site selects
Oryza sativa L. can be maintained at 23.5 DEG C from the formation stage of pistil and stamen to pollen mother cell meiosis (i.e. the 4-6 phase of ear differentiation) temperature
Following place, carries out fertility screening, select that plant stalk is sturdy, tiller is strong, upright blade, leaf angle are little, more than grain number per spike not
Educating is individual plant, carries out anthocyanin content mensure to the brown rice of the individual plant chosen seeds, selects the individual plant of anthocyanin content, but selected
Individual plant anthocyanin colour value be below 20；
4) f7In generation, is observed by the anniversary that strain plantation carries out system to the individual plant chosen, and selects critical temperature low and stable
The stable strain of sterile phase length.
5) in growth cabinet, the sterile changeover temperature of chosen strain is identified, select sterile line origin temp
≤ 22.5 DEG C of strain d47s.
22.5 DEG C of the black rice two-line sterile line d47s critical temperature of institute's selection-breeding, stable fertility and steady sterile period is long, plant
Stalk is sturdy for strain, tiller is strong, upright blade, leaf angle are little, but its brown rice anthocyanin colour value is only 15.23.
Embodiment 3:
1) 16.37 black rice " Sanguis Anas domestica is glutinous " is reached as male parent with anthocyanin colour value, with temperature sensitive two-line sterile line 272s as mother
This, carry out artificial hybridization, plantation hybridization f1Generation；
2) plant f2Generation, f2For more than 2000 plants of colony, planting site selects Oryza sativa L. from the formation stage of pistil and stamen to pollen mother cell
Reduction division phase (i.e. the 4-6 phase of ear differentiation) temperature can be maintained at less than 23.5 DEG C of place, carries out fertility screening, selects to plant
Stalk is sturdy for strain, tiller is strong, upright blade, leaf angle are little, the sterile line individual plant more than grain number per spike, and the brown rice of the individual plant chosen seeds is entered
Row anthocyanin content measures, and selects the individual plant of anthocyanin content, but selected individual plant anthocyanin colour value is below 20；
3) f3~f6From generation to generation, each is planted by strain to the individual plant chosen from generation to generation, 42 plants of each strain, and planting site selects
Oryza sativa L. can be maintained at 23.5 DEG C from the formation stage of pistil and stamen to pollen mother cell meiosis (i.e. the 4-6 phase of ear differentiation) temperature
Following place, carries out fertility screening, select that plant stalk is sturdy, tiller is strong, upright blade, leaf angle are little, more than grain number per spike not
Educating is individual plant, carries out anthocyanin content mensure to the brown rice of the individual plant chosen seeds, selects the individual plant of anthocyanin content, but selected
Individual plant anthocyanin colour value be below 20；
4) f7In generation, is observed by the anniversary that strain plantation carries out system to the individual plant chosen, and selects critical temperature low and stable
The stable strain of sterile phase length.
5) in growth cabinet, the sterile changeover temperature of chosen strain is identified, select sterile line origin temp
≤ 22.5 DEG C of strain d4s.
22.5 DEG C of the black rice two-line sterile line d4s critical temperature of institute's selection-breeding, stable fertility and steady sterile period is long, plant
Stalk is sturdy, tiller is strong, upright blade, leaf angle are little, but its brown rice anthocyanin colour value is only 17.80.
The black rice two-line sterile line contrast and experiment of table 1 embodiment 1-3 institute selection-breeding
As seen from Table 1, the black rice two-line sterile line brown rice anthocyanin colour value of 1 selection-breeding of embodiment reaches 24.29, is assembled
Black rice hybridization rice anthocyanin colour value to reach 20.99(higher than general rice more than 30.5 times, more black than choose at random on market
Rice is high more than 0.8 times).
Additionally, contrasted visible by embodiment 1 and embodiment 2,3, the black rice two-line sterile line of 1 selection-breeding of embodiment is rough
Rice anthocyanin colour value 2,3 0.59 times, 0.36 times of embodiment of ratio respectively, and flavones content is also than embodiment 2,3 respectively 0.45
Again, 0.27 times；The hybrid paddy rice brown rice anthocyanin color that the black rice two-line sterile line of 1 selection-breeding of embodiment and Xinyang fructus zizaniae caduciflorae are assembled
Valency is respectively than 2,3 0.76 times, 0.38 times of embodiment；The black rice two-line sterile line of 1 selection-breeding of embodiment and Xinyang fructus zizaniae caduciflorae institute
The super middle advantage of hybrid paddy rice assembling is 43.05%, the black rice two-line sterile line of 2,3 selection-breeding of embodiment and Xinyang fructus zizaniae caduciflorae institute group
The super middle advantage of hybrid paddy rice joined is respectively -12.72%, 10.88%, shows the black rice two-line sterile line institute of 1 selection-breeding of embodiment
The black rice brown rice anthocyanin colour value assembling has obvious hybrid vigor.
Present invention determine that selection-breeding is rich in anthocyanin black rice two-line sterile line selection, the selection of parent is the present invention
Key.Brown rice anthocyanin colour value can be selected by the present invention and reach that more than 20, stable fertility, critical temperature be low and stable sterile
Phase length, plant stalk are sturdy, tiller is strong, upright blade, leaf angle are little, the black rice two-line sterile line more than grain number per spike, institute's selection-breeding
Rich in the hybrid that anthocyanin two-line sterile line is assembled, hybrid vigor is not only had on yield, in nutrition such as anthocyanin, flavone
Hybrid vigor is also had on composition.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modify, all should belong to the covering scope of the present invention.
Claims (2)
1. a kind of selection of the temperature sensitive two-line sterile line of black rice rich in anthocyanin it is characterised in that: male parent selects brown rice
Anthocyanin colour value reaches more than 20 black rice, and female parent is selected temperature sensitive two-line sterile line, carried out artificial hybridization, from f2In generation, starts per generation
All carry out the selection of three reselection procedure anthocyanin contents, the selection of fertility, the selection of economical character, final selection-breeding becomes rich in flower
The temperature sensitive two-line sterile line of black rice of color glycosides, the temperature sensitive two-line sterile line anthocyanin content of described black rice, stable fertility, critical
Temperature is low and steady sterile period length, plant stalk are sturdy, tiller is strong, upright blade, leaf angle are little, grain number per spike is many；
Described male parent selects black rice " nantan84 "；
Described female parent selects temperature sensitive two-line sterile line 272s.
2. according to claim 1 be rich in anthocyanin the temperature sensitive two-line sterile line of black rice selection it is characterised in that:
Described selection comprises the following steps:
1) more than 20 black rice is reached as male parent with brown rice anthocyanin colour value, with temperature sensitive two-line sterile line as female parent, carry out artificial
Hybridization, plantation hybridization f1Generation；
2) plant f2Generation, f2For more than 2000 plants of colony, Oryza sativa L. i.e. children from the formation stage of pistil and stamen to pollen mother cell meiosis
The temperature of the 4-6 phase of Spike development, not above 23.5 DEG C, carries out fertility screening, selection plant stalk is sturdy, tiller is strong, blade is straight
Vertical, leaf angle is little, the sterile line individual plant more than grain number per spike, carries out anthocyanin content mensure to the brown rice of the individual plant chosen seeds, selects flower
The individual plant of color glycosides content；
3) f3~f6From generation to generation, each is pressed strain to individual plant selected by upper one generation from generation to generation and plants, and Oryza sativa L. is from the formation stage of pistil and stamen to pollen
Blast cell be reduction division phase the temperature of the 4-6 phase of ear differentiation not above 23.5 DEG C, carry out fertility screening, select plants stems
Stalk is sturdy, tiller is strong, upright blade, leaf angle are little, the sterile line individual plant more than grain number per spike, and the brown rice of the individual plant chosen seeds is spent
Color glycosides assay, selects the individual plant of anthocyanin colour value content；
4) f7The individual plant of the anthocyanin content that generation is chosen to step 3) is planted by strain, selects critical temperature low and stablizes not
Educate the stable strain of phase length；
5) in growth cabinet, the sterile changeover temperature of chosen strain is identified, selection sterile line origin temp≤
22.5 DEG C of strain, that is, be bred as the temperature sensitive two-line sterile line of black rice rich in anthocyanin.
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| CN107047283B (en) * | 2017-03-22 | 2019-03-12 | 上海交通大学 | A kind of take color color value as the pyramiding breeding method of colored hyacinth bean breeding standard |
| CN106857233A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of two is the breeding method of Black rice sterile line |
| CN106942046A (en) * | 2017-03-22 | 2017-07-14 | 湖南杂交水稻研究中心 | A kind of two be the breeding method of Glutinous CMS line |
| CN109042289B (en) * | 2018-07-17 | 2021-08-03 | 浙江大学 | Breeding method of black endosperm high-anthocyanin rice |
| CN109618917B (en) * | 2019-02-02 | 2022-09-06 | 福建农林大学 | Breeding method of long grain odor type disease-resistant high-quality black rice three-line sterile line |
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| CN1465228A (en) * | 2002-07-03 | 2004-01-07 | 杨长登 | Breeding method for high-yields colored brown rice |
| CN102524047A (en) * | 2011-12-26 | 2012-07-04 | 杨玉梁 | Cultivation and product production method of natural vitamin A containing fragrant rice type photo-thermo-sensitive sterile line and restorer line of special rice |
| CN102524052B (en) * | 2012-01-11 | 2013-01-09 | 福建农林大学 | Breeding method for producing paddy rice with colorful rice producing function |
| CN102742498A (en) * | 2012-06-18 | 2012-10-24 | 芦溪县一村食品有限责任公司 | Cultivation method of hybrid high-quality purple rice seed |
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