It is a kind of to induce the method that the female tree bud differentiation of red bayberry is male flower
Technical field
The invention belongs to the important step of red bayberry crossbreeding, is related to a large amount of sources of red bayberry crossbreeding offer and understands,
The key technology and concrete operation method of the clear and definite red bayberry pollen of fruit phenotype, and in particular to one kind induction female tree bud differentiation of red bayberry
For the method for male flower.
Background technology
Red bayberry (Myrica rubra (Lour.) Sieb.et Zucc.) for Myruca ceas, the perennial fruit tree of Myrica(Myrica), often
Green arbor, it is distinct Chinese characteristics economic fruit.One of favorite fruit of summer people, and have and promote the production of body fluid to quench thirst, be aid digestion, relieving the effect of alcohol
Solve the curative effects such as hot summer weather.Waxberry fruit is nutritious, except rich in nutrient necessary to a variety of human bodies such as vitamin, carbohydrate and
Outside the mineral matters such as calcium, phosphorus, sodium, iron, zinc, also containing substantial amounts of anthocyanidin and polyphenols, therefore there is strong freedom
Base Scavenging activity.Waxberry fruit can also be processed into the food such as canned arbutu in syrup, preserved fruit, fruit juice, dried fruit, fruit wine in addition to eating raw,
Therefore red bayberry combines many excellent characteristics, has high economic value.
Red bayberry has the cultivation history of more than 2000 years in China, accounts for whole world red bayberry cultivation face in national cultivated area at present
Product more than 99%, there are the provinces and regions such as Zhejiang, Fujian, Hunan, Jiangsu, Yunnan, Guangxi, Guizhou and Chongqing in Main Cultivation province, due in
State's red bayberry cultivation history is long, and ecological environment is various, thus generates the kind, strain and type of character polyisocyanate.
Red bayberry is dioecism, hermaphrodite phenomenon seldom be present.Larger only more than the ten individual product of cultivated area at present
Kind, it is followed successively by by the order of morning and evening in maturity period:Early shepherd's purse honey plum, big live charcoal plum, the early great Mei that borders on the sea, early color, fourth Ao plums, Western Hills crow
Plum, water chestnut, sweet tea aspen plum, the thin bases of a fruit of great Ye, the thin base of a fruit of leaflet, the crisp core of crow, live charcoal plum, late shepherd's purse honey plum, late rice red bayberry, east chief.Wherein
Water chestnut kind and the cultivated area in the east chief whole nation are maximum.Above all of cultivar is all female plant.
Crossbreeding is to cultivate an important method of new varieties, is that the merit of two or more kinds is passed through into friendship
With concentration together, then by selection and cultivate, obtain new varieties, crossbreeding can not only obtain the new product of concentration parents' advantage
Kind, moreover it is possible to the small gene of many effects is concentrated, and shows the super close phenomenon of quantitative character, current many varieties of crops are all
The new varieties cultivated by crossbreeding.
Carry out the fruit properties of the necessary clear and definite Parent of crossbreeding, could targetedly select male parent and maternal development
Crossbreeding, because red bayberry is dioecism, cause the fruit properties that we can only be clearly maternal, the fruit of male parent cannot be identified
Feature, so as to which crossbreeding can not be carried out by Parent of the wish selection with complementary traits of people, so current red bayberry institute
Some cultivars all pass through bud mutation or suitable different cultivated of changing.
Therefore, by cultivating treatment measures, current red bayberry female plant kind part female flower is transformed into male flower, so that it may be poplar
Plum hybridization offer source understands, the clear and definite paternal pollen of character, so as to ensure that red bayberry carries out the smooth implementation of crossbreeding,
Basis is provided to cultivate the more diversified red bayberry kind for meeting people and the market demand.
The content of the invention
The technical problem to be solved in the present invention is overcome the deficiencies in the prior art, there is provided it is a kind of it is simple and convenient, fast and effective,
It can hybridize for red bayberry and provide that source understands, the clear and definite paternal pollen of character, guarantee red bayberry can carry out the induction poplar of crossbreeding
The female tree bud differentiation of plum is the method for male flower.
In order to solve the above technical problems, the present invention uses following technical scheme:
A kind of to induce the method that the female tree bud differentiation of red bayberry is male flower, methods described is the flower bud differentiation period in the female tree of red bayberry to poplar
The branch of the female tree of plum applies plant growth inhibitor, and induction red bayberry bud differentiation is male inflorescence, the plant growth inhibitor bag
Include the one or more in paclobutrazol, uniconazole P, Prohexadione calcium and Triiodobenzoic acid.
The above-mentioned female tree bud differentiation of induction red bayberry is in the method for male flower, it is preferred that the plant growth inhibitor
Time of application is July~November.
The female tree bud differentiation of above-mentioned induction red bayberry is in the method for male flower, it is preferred that the plant growth inhibitor with
The mode of the aqueous solution containing plant growth inhibitor is administered, when plant growth inhibitor uses paclobutrazol, in the aqueous solution
The concentration of paclobutrazol is 200mg/kg~1200mg/kg, when plant growth inhibitor uses uniconazole P, uniconazole P in the aqueous solution
Concentration be 200mg/kg~1200mg/kg, when plant growth inhibitor uses Prohexadione calcium, Prohexadione calcium in the aqueous solution
Concentration is 60mg/kg~200mg/kg, when plant growth inhibitor uses Triiodobenzoic acid, Triiodobenzoic acid in the aqueous solution
Concentration is 60mg/kg~200mg/kg.Specific concentration can carry out concentration adjustment according to the growth potential difference of tree body, and growth potential is strong
Concentration should be high, otherwise the concentration of plant growth inhibitor should be reduced.
The above-mentioned female tree bud differentiation of induction red bayberry is in the method for male flower, it is preferred that the mode of the administration is to spray.
The above-mentioned female tree bud differentiation of induction red bayberry is in the method for male flower, it is preferred that and the weather of the administration is the cloudy day,
And ensure to rain using nothing in 24 hours after plant growth inhibitor.
The above-mentioned female tree bud differentiation of induction red bayberry is in the method for male flower, it is preferred that the object of the administration is red bayberry
The part branch of female tree, it is impossible to which whole strain is applied.
The kind of the female tree of red bayberry of the present invention includes cultivar or wild varieties.
Compared with prior art, the advantage of the invention is that:
1st, method of the invention carries out regulation and control processing using plant growth inhibitor in flower bud differentiation period to the female branch bar of red bayberry, into
It is male inflorescence to induce red bayberry bud differentiation work(, so as to for red bayberry hybridization provide source understand, the clear and definite male parent flower of character
Powder, ensure that red bayberry carries out the smooth implementation of crossbreeding, basis is provided to cultivate more diversified red bayberry kind.The present invention is logical
The selection of plant growth inhibitor is crossed, i.e., by carrying out spraying examination to vegetable depressors such as paclobutrazol, uniconazole P and Prohexadione calciums
Test and show, it is male inflorescence to suppress the growth regulator of plant growth to be catalyzed bud differentiation during red bayberry bud differentiation.
2nd, time of application must be administered before blooming in method of the invention, i.e., during bud differentiation, typically
The latter moon is plucked to before then for Waxberry fruit, i.e. 7-11 months.
3rd, in method of the invention application concentration select plant growth inhibitor for paclobutrazol and/or uniconazole P when, it is dense
Spend for 200mg/kg~1200mg/kg, when plant growth inhibitor is Prohexadione calcium and/or Triiodobenzoic acid, concentration 60mg/
Kg~200mg/kg, preferably it can be divided into male flower by floral bud induction, and to tree body without any damage, Second Year is without growth
In the case of inhibitor processing, can normal differentiation be female flower, normal outcome.
4th, method of the invention all has extensive adaptability to different cultivars, to current cultivar or wild
The female tree of red bayberry all has universal adaptability, but different cultivars growth potential has certain difference, therefore uses growth inhibitor
Concentration there is also certain difference, but the present invention concentration range in be attained by promote bud differentiation into male flower mesh
Mark.
5th, method of the invention can only carry out spraying processing to the branch of female tree, and it is male flower to play the female tree bud differentiation of induction
Effect, it is impossible to whole strain or plant are sprayed on a large scale, using improper whole tree body may be caused weak, in addition induction dead-arm
It is dead.
Brief description of the drawings
Fig. 1 is the red bayberry female inflorescence photo normally bloomed.
Fig. 2 is the photo that red bayberry bud partial differentiation is male inflorescence in the embodiment of the present invention.
Fig. 3 is the photo that red bayberry bud is all divided into male inflorescence in the embodiment of the present invention.
Embodiment
Below in conjunction with Figure of description and specific preferred embodiment, the invention will be further described, but not therefore and
Limit the scope of the invention.
Material and instrument employed in following examples are commercially available.
Embodiment:
A kind of female tree bud differentiation of induction red bayberry of the invention is the method for male flower, is comprised the following steps:
The 7-11 months during the bud differentiation of the female tree of red bayberry, the present embodiment are specifically implemented in October, plant growth inhibitor are matched somebody with somebody
The aqueous solution containing plant growth inhibitor is made, when plant growth inhibitor uses paclobutrazol and/or uniconazole P, using 200,
600th, 1200mg/kg concentration, when plant growth inhibitor uses Prohexadione calcium and/or Triiodobenzoic acid, using 60,100,
160mg/kg concentration, after preparing, the spring tip is given birth to then to the female tree of red bayberry with watering can(On branch and blade)Sprayed, blade
Drip, be selected in cloudy progress, and it is no rainy in 24 hours after spray plant growth inhibitor, and Second Year sprays at the beginning of 2 months
Cross bud on the branch of plant growth inhibitor and will differentiate into male flower, acquired pollen, carry out bagging hybridization pollination and show to induce
The male flower gone out has reproduction vigor, can carry out pollination to female flower and grow normal fruit.The tree body and branch sprayed
Do not use within 2 years this method handled after plant will keep original female tree feature, open normal female flower, normal outcome.
Fig. 1 is the situation of blooming for not using plant growth inhibitor to spray red bayberry branch, as illustrated, when blooming
Whole bud differentiation of the branch are red bayberry female flower.
Fig. 2 is to use low-consistency plant growth inhibitor in the present embodiment(For example, it is when paclobutrazol and/or uniconazole P
It is 60mg/kg when 200mg/kg, Prohexadione calcium and/or Triiodobenzoic acid)The situation of blooming after red bayberry branch is sprayed, as schemed institute
Show, the part bud differentiation of red bayberry branch is red bayberry male flower, and part inflorescence keeps the characteristic of the female tree of red bayberry, continues to be divided into red bayberry female
Flower.
Fig. 3 is the present embodiment middle and high concentration plant growth inhibitor(For example, it is 600mg/ when paclobutrazol and/or uniconazole P
It is 160mg/kg when kg, Prohexadione calcium and/or Triiodobenzoic acid)The situation of blooming of red bayberry branch is sprayed, as illustrated, blooming
When be all divided into red bayberry male flower.
The above described is only a preferred embodiment of the present invention, any formal limitation not is made to the present invention.Though
So the present invention is disclosed as above with preferred embodiment, but is not limited to the present invention.It is any to be familiar with those skilled in the art
Member, in the case where not departing from the Spirit Essence of the present invention and technical scheme, all using in the methods and techniques of the disclosure above
Appearance makes many possible changes and modifications to technical solution of the present invention, or is revised as the equivalent embodiment of equivalent variations.Therefore,
Every content without departing from technical solution of the present invention, the technical spirit according to the present invention is to made for any of the above embodiments any simple
Modification, equivalent substitution, equivalence changes and modification, still fall within technical solution of the present invention protection in the range of.