CN101637121A - Method for fast selecting glutinous rice seed variety - Google Patents
Method for fast selecting glutinous rice seed variety Download PDFInfo
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- CN101637121A CN101637121A CN200910034173A CN200910034173A CN101637121A CN 101637121 A CN101637121 A CN 101637121A CN 200910034173 A CN200910034173 A CN 200910034173A CN 200910034173 A CN200910034173 A CN 200910034173A CN 101637121 A CN101637121 A CN 101637121A
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Abstract
A method for fast selecting a glutinous rice seed variety is characterized by selecting a recurrent parent and a donor parent for hybridization, harvesting hybrid seeds F1 of the first generation, andsimultaneously harvesting recurrent parent seeds; repeating the operations till the harvest of six generations of backcross hybrid seeds, and simultaneously harvesting the six generations of backcross hybrid seeds BC6 F1 as well as the recurrent parent; planting the six generations of backcross hybrid seeds BC6 and F1 as well as the recurrent parent; taking down spikes from each plant of the BC6F1 group, carrying out indoor pollen chromoscopy, and eliminating and pulling out single plants with unseparated pollen coloration; when selected plants are single plants extremely similar to the recurrent parent, harvesting the backcross hybrid seeds of the second of the six generations according to each of the single plants; planting various BC6 F2 plants, also planting the recurrent parent forcomparison, selecting seeds from a group extremely similar to the recurrent parent, separately harvesting according to each of the single plants, separately threshing, drying and husking, mixing the seeds of glutinous rice plants so as to form a glutinous rice target variety, and carrying out propagation and typing. The method has high selection speed, increases the possibility of variety breedingsuccess and has low operation cost and a clear target market.
Description
Technical field
The present invention relates to a kind of selection of rice varieties, particularly a kind of method for fast selecting glutinous rice seed variety.
Background technology
The selection of rice varieties of the prior art mainly contains:
1, recessive gene backcross transformation method, classical way is the throwback backcross method in the genetics, but transformation speed is slow, and breeding cycle is long.
2, the rice glutinous trait kind is generally by sticking type and glutinous type inter breed crossing, and with the breeding of pedigree back-and-forth method, this method needs colony big, and the offspring is stable slow, and breeding cycle is long, and the probability of success is low.
3, molecular labeling back-and-forth method: can realize quick backcross transformation, but that laboratory condition requires is high, and cost height, detection time are long.
Summary of the invention
Technical problem to be solved by this invention is at the deficiencies in the prior art, and a kind of workable, cost is low, the cycle is short method for fast selecting glutinous rice seed variety is provided.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of method for fast selecting glutinous rice seed variety, is characterized in, its step is as follows:
(1) selects an outstanding non-glutinous type kind,, select the good glutinous rice variety of a comprehensive proterties, make the donor parents of glutinous trait gene wx as recurrent parent; Recurrent parent is done male parent and donor parents hybridization, results first generation of hybrid seed F
1, gather in the crops the recurrent parent seed simultaneously;
(2) the plantation first generation of hybrid and recurrent parent seed, take recurrent parent as maternal, with first generation of hybrid hybridization, results first backcross generation BC
1F
1With the recurrent parent seed;
(3) plantation first backcross generation BC
1F
1With the recurrent parent seed; To add 0.2 gram iodine I in every 100ml distilled water
2With 2 gram KI KI, dissolve, be made into pollen staining liquid fully; Select seemingly individual plant 10 strains of recurrent parent of appearance in the first backcross generation colony, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy; Every fringe is got each 2 grain husk flowers of upper, middle and lower, takes out wherein flower pesticide, puts on the slide, adds one of pollen staining liquid, smashes up flower pesticide, and the test under microscope of 100 times of multiplication factors is put in film-making; When pollen staining in the visual field obviously is depth separation, illustrate that then this individual plant is the glutinous trait gene heterozygous, selecting this individual plant is that male parent and recurrent parent are hybridized, results second backcross generation hybrid BC
2F
1With the recurrent parent seed;
(4) plantation second backcross generation hybrid BC
2F
1With the recurrent parent seed; Repeating step (3) microscopy is selected the individual plant of glutinous trait gene heterozygosis to backcross again and is produced the third backcross generation hybrid; Repeat operation, until results backcross six generation hybrid, results six generation hybrid BC that backcross simultaneously
6F
1With recurrent parent;
(5) plant the six generation hybrid BC that backcross
6F
1With recurrent parent; BC
6F
1Get fringe by strain in the colony, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the utmost point like the individual plant of recurrent parent, singly receives, singly shines, singly goes out rough by strain, and selecting wherein, waxy grain is mixed into the six generation second generation of hybrid BC that backcross
6F
2Seed;
(6) plantation BC
6F
2Each strain, waxy strain divide system and recurrent parent to carry out the residential quarter contrast, and through system identification, the final election major clique recommends to participate in the variety plot examination.
Paddy rice is divided into sticking glutinous two kinds of rice types, and non-glutinous rice is a basic model, shows as dominance, and genotype is WxWx or Wxwx (wx is cured plasmagene, and Wx is the allelomorph of wx); Glutinous rice is non-basic model, shows as recessiveness, and genotype is wxwx.Wxwx type individual plant can be isolated two kinds of gamete: Wx, wx, two kinds of gamete I
2-KI equilibrium liquid dyeing performance is different, and Wx genotype pollen staining is black, and wx genotype pollen staining is shallow, namely two kinds of dyeing types significantly occur on individual plant pollen staining eyeglass, and it is the Wxwx genotype that the individual plant of two kinds of dyeing pollen grains is arranged.Select the hybridization of Wxwx genotype and sticking type recurrent parent, can be combined into two kinds of genotype: WxWx, Wxwx.The individual plant that WxWx type pollen staining does not separate, pollen staining is black is eliminated, and Wxwx genotype pollen staining separates, with backcross as male parent.Backcross 4-5 time, in inbreeding population, select wxwx homozygous genotype (glutinous rice) individual plant then, breed the glutinous rice variety similar to recurrent parent.With the inventive method transformation glutinous rice variety, similar dominant gene backcross transformation method can be carried out the continuous backcross transformation, does not need selfing to identify, can shorten the seed selection time of glutinous rice variety effectively.
Recurrent parent described in the present invention can be that sticking type kind is successfully promoted in one in the target market, is the output height of newly breeding, the kind that Comprehensive Traits is good.Whether the number of times of backcrossing among the present invention has been satisfied with on the similarity of a backcross hybrid generation and recurrent parent and has been decided.When finishing, still need pseudostationary is pulled out in hybrid pollen chromoscopy when backcrossing.True hybrid plant division goes out rough, chooses glutinous property grain, and the plant division mixing is built and is.Respectively be to compare with recurrent parent, the glutinous type isogenic line of final election supplies to participate in the experiment, marketing is used.
Compared with prior art, the present invention has the following advantages:
1, breeding speeds up: can utilize Hainan Island to add the condition in generation winter, realize backcrossing in season more than a year, round-grained rice type kind can plant for three seasons in 1 year, if manual light control's condition is arranged, can realize backcrossing throughout the year, so this method biggest advantage is quick.Just can the finalize the design glutinous type isogenic line of good sticking type kind of general triennium, fast two years of person also can become good glutinous type kind and glue the type isogenic line.
2, improved variety possibility increases: use this law seed selection glutinous rice variety, as long as the kind that recurrent parent is high yield, Comprehensive Traits is good, target variety is easily seed selection success just.
3, implementation cost is low: this method can be omitted molecule laboratory and its person skilled without Markers for Detection, as long as be equipped with microscope and relevant necessary instrument and medicament, simple to operate, cost is low, and detection speed is fast, and accurately.
4, the target market is clear and definite: can carry out orderly improvement at the adapted breed in the target market, breeding objective is clear and definite, and is workable, and improved variety can be promoted fast.This breeding method comes down to a kind of kind quick improvement method.
Description of drawings
Fig. 1 is a kind of breeding schematic flow sheet of the present invention.Among the figure: BCn represents to backcross n time, and Fm represents hybrid m generation.
Embodiment
Referring to accompanying drawing, further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not consist of its Copyright law.
Embodiment 1.With reference to Fig. 1.A kind of method for fast selecting glutinous rice seed variety, its step is as follows:
(1) selects an outstanding non-glutinous type kind,, select the good glutinous rice variety of a comprehensive proterties, make the donor parents of glutinous trait gene wx as recurrent parent; Recurrent parent is done male parent and donor parents hybridization, results first generation of hybrid seed F
1, gather in the crops the recurrent parent seed simultaneously;
(2) the plantation first generation of hybrid and recurrent parent seed, take recurrent parent as maternal, with first generation of hybrid hybridization, results first backcross generation BC
1F
1With the recurrent parent seed;
(3) plantation first backcross generation BC
1F
1With the recurrent parent seed; To add 0.2 gram iodine I in every 100ml distilled water
2With 2 gram KI KI, dissolve, be made into pollen staining liquid fully; Select seemingly individual plant 10 strains of recurrent parent of appearance in the first backcross generation colony, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy; Every fringe is got each 2 grain husk flowers of upper, middle and lower, takes out wherein flower pesticide, puts on the slide, adds one of pollen staining liquid, smashes up flower pesticide, and the test under microscope of 100 times of multiplication factors is put in film-making; When pollen staining in the visual field obviously is depth separation, illustrate that then this individual plant is the glutinous trait gene heterozygous, selecting this individual plant is that male parent and recurrent parent are hybridized, results second backcross generation hybrid BC
2F
1With the recurrent parent seed;
(4) plantation second backcross generation hybrid BC
2F
1With the recurrent parent seed; Repeating step (3) microscopy is selected the individual plant of glutinous trait gene heterozygosis to backcross again and is produced the third backcross generation hybrid; Repeat operation, until results backcross six generation hybrid, results six generation hybrid BC that backcross simultaneously
6F
1With recurrent parent;
(5) plant the six generation hybrid BC that backcross
6F
1With recurrent parent; BC
6F
1Get fringe by strain in the colony, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the utmost point like the individual plant of recurrent parent, singly receives, singly shines, singly goes out rough by strain, and selecting wherein, waxy grain is mixed into the six generation second generation of hybrid BC that backcross
6F
2Seed;
(6) plantation BC
6F
2Each strain, waxy strain divide system and recurrent parent to carry out the residential quarter contrast, and through system identification, the final election major clique recommends to participate in the variety plot examination.
Embodiment 2.With reference to Fig. 1.A kind of method for fast selecting glutinous rice seed variety, its step is as follows:
(1) selects an outstanding non-glutinous type kind such as Xu rice No. 3, as recurrent parent, select the good glutinous rice variety Anhui rice 68 of a Comprehensive Traits, make the donor parents of glutinous trait gene wx; Xu rice does male parent and donor parents hybridization for No. 3, results first generation of hybrid seed F
1, gather in the crops simultaneously No. 3 seeds of Xu rice;
(2) the plantation first generation of hybrid and Xu's rice No. 3 seeds for maternal, with first generation of hybrid hybridization, are gathered in the crops first backcross generation BC take Xu rice No. 3
1F
1With No. 3 seeds of Xu rice;
(3) plantation first backcross generation BC
1F
1With No. 3 seeds of Xu rice; To add 0.2 gram iodine I in every 100ml distilled water
2With 2 gram KI KI, dissolve, be made into pollen staining liquid fully; Select appearance in the first backcross generation colony like individual plant 10 strains of Xu rice No. 3, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy; Every fringe is got each 2 grain husk flowers of upper, middle and lower, takes out wherein flower pesticide, puts on the slide, adds one of pollen staining liquid, smashes up flower pesticide, and the test under microscope of 100 times of multiplication factors is put in film-making; When pollen staining in the visual field obviously is depth separation, illustrate that then this individual plant is the glutinous trait gene heterozygous, selecting this individual plant is that male parent is hybridized for No. 3 with Xu rice, results second backcross generation hybrid BC
2F
1With No. 3 seeds of Xu rice;
(4) plantation second backcross generation hybrid BC
2F
1With No. 3 seeds of Xu rice; Repeating step (3) microscopy is selected the individual plant of glutinous trait gene heterozygosis to backcross again and is produced the third backcross generation hybrid; Repeat operation, until results backcross five generation hybrid, the results six generation hybrid BC that backcross
6F
1With Xu rice No. 3;
(5) plant the six generation hybrid BC that backcross
6F
1With Xu rice No. 3; BC
6F
1Get fringe by strain in the colony, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the utmost point like the individual plant of Xu rice No. 3, by the single harvest of the strain six generation second generation of hybrid BC that backcross
6F
2Seed;
(6) plantation BC
6F
2Each strain, waxy strain divide system and recurrent parent to carry out the residential quarter contrast, and through system identification, the final election major clique recommends to participate in the variety plot examination.
Claims (1)
1, a kind of method for fast selecting glutinous rice seed variety is characterized in that, its step is as follows:
(1) selects an outstanding non-glutinous type kind,, select the good glutinous rice variety of a comprehensive proterties, make the donor parents of glutinous trait gene wx as recurrent parent; Recurrent parent is done male parent and donor parents hybridization, results first generation of hybrid seed F
1, gather in the crops the recurrent parent seed simultaneously;
(2) the plantation first generation of hybrid and recurrent parent seed, take recurrent parent as maternal, with first generation of hybrid hybridization, results first backcross generation BC
1F
1With the recurrent parent seed;
(3) plantation first backcross generation BC
1F
1With the recurrent parent seed; To add 0.2 gram iodine I in every 100ml distilled water
2With 2 gram KI KI, dissolve, be made into pollen staining liquid fully; Select seemingly individual plant 10 strains of recurrent parent of appearance in the first backcross generation colony, get the fringe that just goes out fringe 1/2-1/3, take back indoor microscopy; Every fringe is got each 2 grain husk flowers of upper, middle and lower, takes out wherein flower pesticide, puts on the slide, adds one of pollen staining liquid, smashes up flower pesticide, and the test under microscope of 100 times of multiplication factors is put in film-making; When pollen staining in the visual field obviously is depth separation, illustrate that then this individual plant is the glutinous trait gene heterozygous, selecting this individual plant is that male parent and recurrent parent are hybridized, results second backcross generation hybrid BC
2F
1With the recurrent parent seed;
(4) plantation second backcross generation hybrid BC
2F
1With the recurrent parent seed; Repeating step (3) microscopy is selected the individual plant of glutinous trait gene heterozygosis to backcross again and is produced the third backcross generation hybrid; Repeat operation, until results backcross six generation hybrid, results six generation hybrid BC that backcross simultaneously
6F
1With recurrent parent;
(5) plant the six generation hybrid BC that backcross
6F
1With recurrent parent; BC
6F
1Get fringe by strain in the colony, indoor pollen staining inspection, superseded pollen staining does not separate individual plant, and pulls out; Elected selecting good strains in the field for seed selected the utmost point like the individual plant of recurrent parent, singly receives, singly shines, singly goes out rough by strain, and selecting wherein, waxy grain is mixed into the six generation second generation of hybrid BC that backcross
6F
2Seed;
(6) plantation BC
6F
2Each strain, waxy strain divide system and recurrent parent to carry out the residential quarter contrast, and through system identification, the final election major clique recommends to participate in the variety plot examination.
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|---|---|---|---|
| CN200910034173A CN101637121A (en) | 2009-09-02 | 2009-09-02 | Method for fast selecting glutinous rice seed variety |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102396415A (en) * | 2011-09-05 | 2012-04-04 | 连云港市农业科学院 | Sterilant herbicide-resistant glutinous rice rapid breeding method |
| CN102405827A (en) * | 2011-08-16 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
| CN102405828A (en) * | 2011-08-19 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of glutinous-rice sterile line of three lines |
| CN102630557A (en) * | 2012-05-12 | 2012-08-15 | 福建农林大学 | Method for breeding functional formula rice varieties |
| CN104396723A (en) * | 2014-10-11 | 2015-03-11 | 福建农林大学 | Method of reducing content of amylase in hybrid rice |
| CN104823657A (en) * | 2015-04-30 | 2015-08-12 | 黔西南布依族苗族自治州农业科学研究所 | Breeding method for glutinous coix |
| CN105325283A (en) * | 2015-09-28 | 2016-02-17 | 杭州中泽生物科技有限公司 | Breeding method of health-care paddy rice variety |
| CN108388766A (en) * | 2018-01-19 | 2018-08-10 | 江汉大学 | A kind of method that molecular difference is identified between kind |
-
2009
- 2009-09-02 CN CN200910034173A patent/CN101637121A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405827A (en) * | 2011-08-16 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
| CN102405828A (en) * | 2011-08-19 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of glutinous-rice sterile line of three lines |
| CN102396415A (en) * | 2011-09-05 | 2012-04-04 | 连云港市农业科学院 | Sterilant herbicide-resistant glutinous rice rapid breeding method |
| CN102630557A (en) * | 2012-05-12 | 2012-08-15 | 福建农林大学 | Method for breeding functional formula rice varieties |
| CN102630557B (en) * | 2012-05-12 | 2013-06-19 | 福建农林大学 | Method for breeding functional formula rice varieties |
| CN104396723A (en) * | 2014-10-11 | 2015-03-11 | 福建农林大学 | Method of reducing content of amylase in hybrid rice |
| CN104823657A (en) * | 2015-04-30 | 2015-08-12 | 黔西南布依族苗族自治州农业科学研究所 | Breeding method for glutinous coix |
| CN105325283A (en) * | 2015-09-28 | 2016-02-17 | 杭州中泽生物科技有限公司 | Breeding method of health-care paddy rice variety |
| CN108388766A (en) * | 2018-01-19 | 2018-08-10 | 江汉大学 | A kind of method that molecular difference is identified between kind |
| CN108388766B (en) * | 2018-01-19 | 2021-11-16 | 江汉大学 | Method for identifying molecular difference between varieties |
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Application publication date: 20100203 |