CN104396723A - Method of reducing content of amylase in hybrid rice - Google Patents

Method of reducing content of amylase in hybrid rice Download PDF

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CN104396723A
CN104396723A CN201410533003.1A CN201410533003A CN104396723A CN 104396723 A CN104396723 A CN 104396723A CN 201410533003 A CN201410533003 A CN 201410533003A CN 104396723 A CN104396723 A CN 104396723A
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glutinous
seed
rice
maintainer
sowing
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黄荣华
范富英
何水林
章清杞
官德义
李美德
杨帆
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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Abstract

The invention discloses a method of reducing the content of amylase in hybrid rice and belongs to the technical field of rice genetic breeding. In the method, an original hybrid rice combination: one of a parent of a non-glutinous sterile line/a non-glutinous restorer is modified into a glutinous parent. The hybrid rice is prepared through a hybridization method of a glutinous sterile line/non-glutinous restorer or a non-glutinous sterile line/a glutinous restorer to obtain a hybrid rice variety in which the content of the amylase is lower than that of the original hybrid rice. In the invention, by means of a genetic effect and a genetic dosage effect of a glutinous gene wx, a near-isogenic line combination is reduced in the content of the amylase. In addition, a growth period, a plant and leaf form, panicle traits, floral organ traits, yield traits, disease resistant performance, adaptability and the like of the near-isogenic line combination are free of significant different when the near-isogenic line combination is compared with the original hybrid rice combination. Through quality analysis and taste evaluation, a new hybrid rice variety being appropriate in the content of the amylase and having the taste and the quality which are suitable for people in different region, thereby achieving an object of genetically improving a poor taste and a poor quality due to a high amylase content in the hybrid rice in the prior art.

Description

A kind of method reducing hybrid rice rice grain amylose content
Technical field
The present invention is specifically related to a kind of method reducing hybrid rice rice grain amylose content, belongs to Genetic and breeding in rice technical field.
Background technology
The over half a century in past, China's rice breeding experienced by the technological innovation that secondary leads over the world: one is start from the fifties not to change the short stalk rice breeding that plant type is main technological route, and two what be that early seventies starts to apply take heterosis utilization as the hybrid rice breeding of main technological route.Rice yield and total the producing of secondary technological innovation Jun Shi China have been turned over, meeting ever-increasing population to the demand of grain having been made great contribution, achieve noticeable achievement.In recent years, along with the raising of living standards of the people and the adjustment of diet structure, rice consumption is changed by " scalar type " to " mass type " in past, simultaneously, after China joined WTO, the international competition that rice market faces increasingly sharpens, and governments at all levels and consumers in general are concern to rice quality ripple, require that the cry improving rice quality is more and more higher, new higher requirement is proposed to rice quality.The problem of kind (special early indica hybrid rice) the rice matter difference of existing rice breeding technology and institute's seed selection thereof highlights, on the basis of stable high yield, how improving rice quality become current rice breeding technology innovation area important topic urgently to be resolved hurrily, is also one of main direction of China's rice breeding from now on.
Amylose content is the center proterties of rice quality, is also the most important factor affecting rice quality.The usual leading indicator using straight chain shallow lake content as qualification rice quality in the world.In order to obtain the suitable rice varieties of amylose content, some breeding men adopt different amylose content parent to hybridize, and expect to screen desirable kind from offspring's segregative generation, owing to there is Endosperm Genetic Effects and dosage effect of gene, produce little effect.Separately there are some researchers to utilize Protocols in Molecular Biology, adopt transgenic technology by waxy gene wximport kind, although obtain some useful materials, cost is high, transgenosis safe has a big risk, and is also difficult in a short time enter the practical stage.The hybrid rice that current production is applied, the group mode of parent is " non-glutinous male sterile line (A or S)/non-glutinous restorer (R) ", F 1, F 2grain endosperm genotype is WxWxWx, due to edible be F 1seed (the F of Dai Suojie 2), as long as reduce F in theory 2the dosage of Wx in grain endosperm WxWxWx genotype, improves wxdosage, just likely reduce amylose content, thus realize the target improving hybrid rice rice eating-quality.But a difficult problem of realizing this goal is the glutinous cytoplasmic mutation obtaining Wx under the immovable prerequisite of other proterties wxand improve wxdosage.
We with respectively containing Wx or wxhybrid rice parents near-isogenic line make material, take " glutinous male sterile line ( wxa or wxs)/non-glutinous restorer (R) " and " non-glutinous male sterile line (A or S)/glutinous restorer ( wxr) mode " hybridizes combo, can obtain F 1endosperm genotype is respectively Wx wxwxand WxWx wx, F 2endosperm genotype is all wxwxwx, Wx wxwx, WxWx wxwith the colony of WxWxWx tetra-kinds of genotype mixing, ratio is 1:1:1:1, wherein wxwxwxtype shows as glutinous matter (amylose content≤2%), Wx wxwx, WxWx wxbe non-glutinous matter with WxWxWx type appearance, this is consistent with the result of study of forefathers, but due to wxthe dose effect of gene, three there are differences on amylose content, and wherein WxWxWx type amylose content is the highest, WxWx wxtype takes second place, Wx wxwxtype is minimum.Because the amylose content determining rice cooking and eating quality is F 2the aggregate sample of colony, so we predict F 2endosperm genotype comprises wxwxwx, Wx wxwx, WxWx wxwith the hybrid rice rice grain amylose content of WxWxWX tetra-kinds of mixed types than F 2the standard hybridization rice that endosperm genotype is only WxWxWx has and reduces largely, thus realizes the genetic improvement to hybrid rice rice eating-quality.
Summary of the invention
The object of the present invention is to provide a kind of method reducing hybrid rice rice grain amylose content.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method reducing hybrid rice rice grain amylose content, by former Hybrid Rice Combination: one of parent of non-glutinous male sterile line/non-glutinous restorer is transformed into glutinous matter parent, adopt the Crossing system preparing hybrid rice of glutinous male sterile line/non-glutinous restorer or non-glutinous male sterile line/glutinous restorer, obtain the Hybrid Rice Varieties that amylose content decreases than former hybrid rice.
The concrete steps of described method are as follows:
1) one of parent of former Hybrid Rice Combination is transformed into glutinous matter parent:
If a) one of parent is CMS line A, this male sterile line A is transformed into glutinous matter parent, method first corresponding maintainer B is transformed into glutinous matter maintainer wxb, then by backcrossing with the hybridization of former A system, 2-3 generation, transformation Cheng Yuyuan A system only amylose content significant difference, the glutinous matter near-isogenic line male sterile line of other proterties no significant difference wxa;
I. concrete transformation process is as follows: select high yield, high-quality, disease-resistant glutinous rice variety and the maintainer B applied on producing to hybridize, obtain hybridization F 1seed; Sowing F 1with maintainer B, make recurrent parent with maintainer B, complete hybridization F heading stage 1with backcrossing of maintainer B, obtain first backcross generation B 1f 1seed; By B 1f 1go out rough, select glutinous matter brown rice grain sowing wherein, sow maintainer B simultaneously, select breeding time, individual plant that economical character is the most close with maintainer B heading stage, complete B 1f 1backcross with the secondary of maintainer B, obtain BC 1f 1seed; By BC 1f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows maintainer B simultaneously, selects breeding time, individual plant that economical character is the most close with maintainer B heading stage, completes BC 1f 1with backcrossing for three times of maintainer B, obtain BC 2f 1seed; By BC 2f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows maintainer B simultaneously, selects breeding time, individual plant that economical character is the most close with maintainer B heading stage, completes BC 2f 1with backcrossing for four times of maintainer B, obtain BC 3f 1seed; By BC 3f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, BC 3f 1colony's field planting, each Agronomic neat and consistent, and similar to former B system, be namely bred as glutinous matter maintainer wxb; Then the sowing same period wxb and former A system, heading stage completes wxthe hybridization of B system and former A system, obtains hybridization F 1seed; Sowing F 1with wxb, heading stage completes F 1with wxb backcrosses, and obtains B 1f 1seed; By B 1f 1seed goes out rough, and select glutinous matter brown rice grain sowing wherein, the same period is sowed wxb, heading stage completes B 1f 1with wxb backcrosses, and obtains BC 1f 1seed; BC 1f 1waxy gene isozygotys, field planting, each Agronomic neat and consistent, and similar to former A system, is namely bred as glutinous matter near-isogenic line male sterile line wxa, continues to use wxb backcrosses and breeds this glutinous matter near-isogenic line male sterile line.
If b) one of parent is two is photo-thermo-sensitive genic male sterile line S, be transformed into glutinous matter parent, method is as follows: select high yield, high-quality, disease-resistant glutinous rice variety and this male sterile line S applied on producing to hybridize, obtain hybridization F 1seed; Plantation F 1, maturing stage seed is mixed receives F 2; Sowing F 2, select F 2the sterile strain that breeding time in colony, strain Leaf pattern, economical character, sterile gene are similar to former S system, cuts head regeneration, selects the individual plant that Characteristics of Fertility Alteration is consistent with former S system heading stage; Hainan is with to isolate breeding winter the selected rice root and stem of certain plants, individual plant sowing, go out rough, select wherein glutinous matter individual plant sowing, field planting performance breeding time and each economical character similar to former S system, namely be bred as and former S system only amylose content significant difference, the glutinous matter near-isogenic line photo-thermo-sensitive genic male sterile line of the equal no significant difference of other proterties wxs;
If c) one of this parent is restorer R, be transformed into glutinous matter parent, method is as follows: select high yield, high-quality, disease-resistant glutinous rice variety and this restorer R applied on producing to hybridize, obtain hybridization F 1seed; Sowing F 1with R system, make recurrent parent with R system, complete hybridization F heading stage 1with backcrossing of R system, obtain first backcross generation seed B 1f 1; By B 1f 1go out rough, select glutinous matter brown rice grain sowing wherein, sow R system simultaneously, select breeding time, individual plant that economical character is the most close with R system heading stage, complete B 1f 1backcross with the secondary of R system, obtain BC 1f 1seed; By BC 1f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows R system simultaneously, selects breeding time, individual plant that economical character is the most close with R system heading stage, completes BC 1f 1with backcrossing for three times of restorer, obtain BC 2f 1seed; By BC 2f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows R system simultaneously, selects breeding time, individual plant that economical character is the most close with R system heading stage, completes BC 2f 1with backcrossing for four times of R system, obtain BC 3f 1seed; By BC 3f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, BC 3f 1colony's field planting, each Agronomic neat and consistent, and similar to R system, be namely bred as and R system only amylose content significant difference, the glutinous matter near-isogenic line restorer of the equal no significant difference of other proterties wxr;
2) " non-glutinous male sterile line A or S/ glutinous matter near-isogenic line restorer is taked wxr, or glutinous matter near-isogenic line male sterile line wxa or glutinous matter near-isogenic line photo-thermo-sensitive genic male sterile line wxthe non-glutinous restorer R of S/ " Crossing system combo, obtain the Hybrid Rice Varieties that decreases than former hybrid rice of amylose content, F 1grain endosperm genotype is Wx wxwxor WxWx wx, F 2grain endosperm genotype is WxWxWx, WxWx wx, Wx wxwxwith wxwxwxfour kinds of mixed types.
Plant above hybrid rice new combination, results F 2, analyzed by rice matter and the evaluation of boiling food flavor, can amylose content be obtained
Reduce, the improved hybrid rice new varieties of Cooking Quality.The present invention utilizes waxy gene wxhereditary effect and gene dosage
Effect, can obtain except amylose content reduce, other such as breeding time, strain Leaf pattern, panicled characters, FLORAL CHARACTERS OF BIG,
Yield traits, disease resistance, adaptability equalization and former Hybrid Rice Combination do not have the near-isogenic line of notable difference to combine.Pass through product
Matter is analyzed and food flavor evaluation, and can obtain amylose content and be suitable for, Cooking Quality meets the hybridization of different geographical crowd demand
Rice new varieties, thus realize genetic improvement existing hybrid rice rice because of the higher target causing Cooking Quality difference of straight chain shallow lake content.
Beneficial effect of the present invention:
1, the hybrid rice new varieties be bred as and the former hybrid rice difference only on amylose content, other such as breeding time, economical character, panicled characters, yield traits, disease resistance and adaptability etc. are all quite similar, they are a pair near-isogenic line, can promote area directly apply at former hybrid rice.
2, this invention utilizes waxy gene wxhereditary effect and dosage effect of gene, by reducing hybrid rice rice grain amylose content, realize the genetic improvement to current high yield hybrid rice rice quality inferior.
Embodiment
embodiment 1: reducing by three is excellent 3301 amylose contents of High-Yielding Hybrid Rice combination II
method 1: maternal II-32A is transformed into glutinous matter male sterile line
The glutinous rice variety Fujian rock that selection production is applied is glutinous hybridizes with maintainer II-32B, obtains hybridization F 1seed; Sowing F 1and II-32B, make recurrent parent with II-32B, complete hybridization F heading stage 1with backcrossing of II-32B, obtain first backcross generation B 1f 1seed; By B 1f 1go out rough, select glutinous matter brown rice grain sowing wherein, sow II-32B simultaneously, select the individual plant that breeding time, economical character are the most similar to maintainer heading stage, complete B 1f 1backcross with the secondary of II-32B, obtain BC 1f 1seed; By BC 1f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows II-32B simultaneously, selects the individual plant that breeding time, economical character are the most similar to II-32B heading stage, completes BC 1f 1with backcrossing for three times of maintainer, obtain BC 2f 1seed; By BC 2f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows II-32B simultaneously, selects the individual plant that breeding time, economical character are the most similar to maintainer heading stage, completes BC 2f 1with backcrossing for four times of II-32B, obtain BC 3f 1seed; By BC 3f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, BC 3f 1the each proterties neat and consistent of colony's variable rate technology, is glutinous matter maintainer II-32 wxb.The same period sows II-32 wxb and former male sterile line II-32A, heading stage completes II-32 wxthe hybridization of B and II-32A, obtains hybridization F 1; Sowing F 1and II-32 wxb, heading stage completes F 1with II-32 wxb backcrosses, and obtains B 1f 1seed; By B 1f 1seed goes out rough, and select glutinous matter brown rice grain sowing wherein, the same period sows II-32 wxb, heading stage completes B 1f 1with II-32 wxb backcrosses, and obtains BC 1f 1; According to this knowledge of heredity, BC 1f 1waxy gene isozygotys, field planting, each Agronomic neat and consistent, is glutinous matter male sterile line II-32 wxa, continues to use II-32 wxb backcrosses and can breed this glutinous matter male sterile line.This glutinous matter male sterile line II-32 wxa and former male sterile line II-32A (II-32 except amylose content significant difference wxa amylose content is 1.9%, II-32A amylose content is 26.8%), the equal no significant difference of other proterties, both are near-isogenic line each other.Use II-32 wxextensive 3301 combos of A and Fujian, can obtain a kind of hybrid rice new varieties, F 1grain endosperm genotype is Wx wxwx, edible F 2grain endosperm comprises WxWxWx, WxWx wx, Wx wxwx, and wxwxwxfour kinds of genotype, former hybrid rice (II-32A/ Fujian extensive 3301) rice grain amylose content is 22.7%, improved hybrid rice (II-32 wxa/ Fujian extensive 3301) rice grain amylose content only 17.2%.
method 2: be transformed into glutinous matter restorer by extensive for male parent Fujian 3301
Select glutinous No. 6 of glutinous rice variety Hubei Province chaste tree of applying on producing to hybridize with Fujian extensive 3301, obtain hybridization F 1seed; Sowing F 1with Fujian extensive 3301, make recurrent parent with Fujian extensive 3301, complete hybridization F heading stage 1with backcrossing of Fujian extensive 3301, obtain first backcross generation seed B 1f 1; By B 1f 1go out rough, select glutinous matter brown rice grain sowing wherein, sow Fujian extensive 3301 simultaneously, select the extensive 3301 the most similar individual plants in breeding time, economical character and Fujian heading stage, complete B 1f 1backcross with the secondary in Fujian extensive 3301, obtain BC 1f 1seed; By BC 1f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows Fujian extensive 3301 simultaneously, selects the extensive 3301 the most similar individual plants in breeding time, economical character and Fujian heading stage, completes BC 1f 1with backcrossing for three times of Fujian extensive 3301, obtain BC 2f 1seed; By BC 2f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows Fujian extensive 3301 simultaneously, selects the extensive 3301 the most similar individual plants in breeding time, economical character and Fujian heading stage, completes BC 2f 1with backcrossing for four times of R system, obtain BC 3f 1seed; By BC 3f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, BC 3f 1colony's field planting, each Agronomic neat and consistent, with reference to former restorer Fujian extensive 3301 breeding times, strain Leaf pattern, panicled characters, flowering habit, pollen amount, loose powder characteristic, select 3301 the most similar individual plants extensive to Fujian, eliminate bad individual plant, through 2-3 from generation to generation field plot test, extensive 3301 only amylose content significant differences can be bred as with Fujian, the glutinous matter near-isogenic line restorer Fujian extensive 3301 of the equal no significant difference of other proterties wxextensive 3301 amylose contents in R(Fujian are 17.5%, Fujian extensive 3301 wxr amylose content is 1.6%).With II-32A and Fujian extensive 3301 wxr combo, can obtain a kind of hybrid rice new varieties, F 1grain endosperm genotype is WxWx wx, edible F 2grain endosperm comprises WxWxWx, WxWx wx, Wx wxwx, and wxwxwxfour kinds of genotype, former hybrid rice (II-32A/ Fujian extensive 3301) rice grain amylose content is 22.7%, improved hybrid rice (II-32A/ Fujian extensive 3301 wxr) rice grain amylose content only 17.5%.
embodiment 2: reducing by three is excellent 527 amylose contents of High-Yielding Hybrid Rice combination D
method 1: maternal D62A is transformed into glutinous matter male sterile line
The glutinous rice variety Fujian rock that selection production is applied is glutinous hybridizes with maintainer D62B, obtains hybridization F 1seed; Sowing F 1and D62B, make recurrent parent with D62B, complete hybridization F heading stage 1with backcrossing of D62B, obtain first backcross generation B 1f 1seed; By B 1f 1go out rough, select glutinous matter brown rice grain sowing wherein, sow D62B simultaneously, select the individual plant that breeding time, economical character are the most similar to D62B heading stage, complete B 1f 1backcross with the secondary of D62B, obtain BC 1f 1seed; By BC 1f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows D62B simultaneously, selects the individual plant that breeding time, economical character are the most similar to II-32B heading stage, completes BC 1f 1with backcrossing for three times of maintainer, obtain BC 2f 1seed; By BC 2f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows D62B simultaneously, selects the individual plant that breeding time, economical character are the most similar to D62B heading stage, completes BC 2f 1with backcrossing for four times of II-32B, obtain BC 3f 1seed; By BC 3f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, BC 3f 1colony's field planting, each Agronomic neat and consistent, is namely bred as glutinous matter maintainer D62 wxb.The same period sows D62 wxb and former male sterile line D62A, heading stage completes D62 wxthe hybridization of B and D62A, obtains hybridization F 1; Sowing F 1and D62 wxb, heading stage completes F 1with D62 wxb backcrosses, and obtains B 1f 1seed; By B 1f 1seed goes out rough, and select glutinous matter brown rice grain sowing wherein, the same period sows D62 wxb, heading stage completes B 1f 1with D62 wxb backcrosses, and obtains BC 1f 1seed; According to this knowledge of heredity, BC 1f 1waxy gene isozygotys, each Agronomic neat and consistent of field planting, is namely bred as glutinous matter male sterile line D62 wxa, continues to use D62 wxb backcrosses and can breed this glutinous matter male sterile line, this glutinous matter male sterile line D62 wxexcept amylose content significant difference, (D62A amylose content is 27.5%, D62 to A and former male sterile line D62A wxa amylose content is 1.5%), the equal no significant difference of other proterties, both are near-isogenic line each other.Use D62 wxextensive 527 combos of A and another name for Sichuan Province, can obtain a kind of hybrid rice new varieties, F 1grain endosperm genotype is Wx wxwx, edible F 2grain endosperm comprises WxWxWx, WxWx wx, Wx wxwx, and wxwxwxfour kinds of genotype, former hybrid rice (D62A/ another name for Sichuan Province extensive 527) rice grain amylose content is 22.7%, improved hybrid rice (D62 wxa/ another name for Sichuan Province extensive 527) rice grain amylose content only 16.5%.
method 2: be transformed into glutinous matter restorer by extensive for male parent another name for Sichuan Province 527
Select glutinous No. 6 of glutinous rice variety Hubei Province chaste tree of applying on producing to hybridize with another name for Sichuan Province extensive 527, obtain hybridization F 1seed; Sowing F 1with another name for Sichuan Province extensive 527, make recurrent parent with another name for Sichuan Province extensive 527, complete hybridization F heading stage 1with backcrossing of another name for Sichuan Province extensive 527, obtain first backcross generation seed B 1f 1; By B 1f 1go out rough, select glutinous matter brown rice grain sowing wherein, sow another name for Sichuan Province extensive 527 simultaneously, select the extensive 527 the most similar individual plants in breeding time, economical character and another name for Sichuan Province heading stage, complete B 1f 1backcross with the secondary in another name for Sichuan Province extensive 527, obtain BC 1f 1seed; By BC 1f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows another name for Sichuan Province extensive 527 simultaneously, selects the extensive 527 the most similar individual plants in breeding time, economical character and another name for Sichuan Province heading stage, completes BC 1f 1with backcrossing for three times of another name for Sichuan Province extensive 527, obtain BC 2f 1seed; By BC 2f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows another name for Sichuan Province extensive 527 simultaneously, selects the extensive 527 the most similar individual plants in breeding time, economical character and another name for Sichuan Province heading stage, completes BC 2f 1with backcrossing for four times of R system, obtain BC 3f 1seed; By BC 3f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, BC 3f 1colony's field planting, each Agronomic neat and consistent, with reference to former restorer another name for Sichuan Province extensive 527 breeding times, strain Leaf pattern, panicled characters, flowering habit, pollen amount, loose powder characteristic, select 527 the most similar individual plants extensive to another name for Sichuan Province, eliminate bad individual plant, through 2-3 from generation to generation field plot test, the glutinous matter near isogene restorer another name for Sichuan Province extensive 527 of extensive 527 only amylose content significant differences can be bred as with another name for Sichuan Province wxextensive 527 amylose contents in R(another name for Sichuan Province are 17.2%, another name for Sichuan Province extensive 527 wxr amylose content is 1.7%).With D62A and another name for Sichuan Province extensive 527 wxr combo, can obtain a kind of hybrid rice new varieties, F 1grain endosperm genotype is WxWx wx, edible F 2grain endosperm comprises WxWxWx, WxWx wx, Wx wxwx, and wxwxwxfour kinds of genotype, former hybrid rice (D62A/ another name for Sichuan Province extensive 527) rice grain amylose content is 22.7%, improved hybrid rice (D62 wxa/ another name for Sichuan Province extensive 527 wxr) rice grain amylose content only 17.2%
embodiment 3: reducing by two is High-Yielding Hybrid Rice combination two line system amylose content
method 1: maternal Peiai 64S is transformed into glutinous matter male sterile line
Select glutinous No. 6 of glutinous rice variety Hubei Province chaste tree of applying on producing to hybridize with Peiai 64S, obtain hybridization F 1seed; Plantation F 1, maturing stage seed is mixed receives F 2; Sowing F 2, select F 2the sterile strain that breeding time in colony, strain Leaf pattern, economical character, sterile gene are the most similar to Peiai 64S, cuts head regeneration, selects the individual plant that Characteristics of Fertility Alteration is consistent with Peiai 64S heading stage; By on select the rice root and stem of certain plants to be with winter Hainan to isolate breeding, individual plant sowing, goes out rough, selects wherein glutinous matter individual plant, is glutinous matter photo-thermo-sensitive genic male sterile line (Peiai 64S wxs).Peiai 64S wxs is compared with Peiai 64S, and except amylose content significant difference, (Peiai 64S amylose content is 22.5% short 64S wxs amylose content 1.9%), the equal no significant difference of other proterties, both are near-isogenic line each other; Use Peiai 64S wxs and 9311 combos, can obtain a kind of hybrid rice new varieties, F 1grain endosperm genotype is Wx wxwx, edible F 2grain endosperm comprises WxWxWx, WxWx wx, Wx wxwx, and wxwxwxfour kinds of genotype, former hybrid rice (Peiai 64S/9311) rice grain amylose content is 21.2%, improved hybrid rice (Peiai 64 wxs/9311) rice grain amylose content only 16.4%.
method 2: male parent 9311 is transformed into glutinous matter restorer
Select glutinous No. 6 of glutinous rice variety Hubei Province chaste tree of applying on producing to hybridize with 9311, obtain hybridization F 1seed; Sowing F 1with 9311, make recurrent parent with 9311, complete hybridization F heading stage 1with 9311 backcross, obtain first backcross generation seed B 1f 1; By B 1f 1go out rough, select glutinous matter brown rice grain sowing wherein, sow 9311 simultaneously, select the individual plant that breeding time, economical character are the most similar to 9311 heading stage, complete B 1f 1backcross with the secondary of 9311, obtain BC 1f 1seed; By BC 1f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows 9311 simultaneously, selects the individual plant that breeding time, economical character are the most similar to 9311 heading stage, completes BC 1f 1with 9311 backcross for three times, obtain BC 2f 1seed; By BC 2f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows 9311 simultaneously, selects the individual plant that breeding time, economical character are the most similar to 9311 heading stage, completes BC 2f 1with backcrossing for four times of R system, obtain BC 3f 1seed; By BC 3f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, BC 3f 1colony's field planting, each the Characters neat and consistent, with reference to former restorer 9311 breeding time, strain Leaf pattern, panicled characters, flowering habit, pollen amount, loose powder characteristic, select the individual plant similar to 9311, eliminate bad individual plant, through 2-3 from generation to generation field plot test, can be bred as with 9311 only amylose content significant difference (9311 amylose contents are 16.5% short 64S wxs amylose content O F1.9%) glutinous matter near isogene restorer 9311 wxr.With Peiai 64S and 9311 wxr combo, can obtain a kind of hybrid rice new varieties, F 1grain endosperm genotype is WxWx wx, edible F 2grain endosperm comprises WxWxWx, WxWx wx, Wx wxwx, and wxwxwxfour kinds of genotype, former hybrid rice (Peiai 64S/9311) rice grain amylose content is 21.2%, improved hybrid rice (Peiai 64S/9311 wxr) rice grain amylose content only 16.9%.

Claims (3)

1. one kind is reduced the method for hybrid rice rice grain amylose content, it is characterized in that: by former Hybrid Rice Combination: one of parent of non-glutinous male sterile line/non-glutinous restorer is transformed into glutinous matter parent, adopt the Crossing system preparing hybrid rice of glutinous male sterile line/non-glutinous restorer or non-glutinous male sterile line/glutinous restorer, obtain the Hybrid Rice Varieties that amylose content decreases than former hybrid rice.
2. the method for reduction hybrid rice rice grain amylose content according to claim 1, is characterized in that: the concrete steps of described method are as follows:
1) one of parent of former Hybrid Rice Combination is transformed into glutinous matter parent:
If a) one of parent is CMS line A, this male sterile line A is transformed into glutinous matter parent, method first corresponding maintainer B is transformed into glutinous matter maintainer wxb, then by backcrossing with the hybridization of former A system, 2-3 generation, transformation Cheng Yuyuan A system only amylose content significant difference, the glutinous matter near-isogenic line male sterile line of other proterties no significant difference wxa;
If b) one of parent is two is photo-thermo-sensitive genic male sterile line S, be transformed into glutinous matter parent, method is as follows: select high yield, high-quality, disease-resistant glutinous rice variety and this male sterile line S applied on producing to hybridize, obtain hybridization F 1seed; Plantation F 1, maturing stage seed is mixed receives F 2; Sowing F 2, select F 2the sterile strain that breeding time in colony, strain Leaf pattern, economical character, sterile gene are similar to former S system, cuts head regeneration, selects the individual plant that Characteristics of Fertility Alteration is consistent with former S system heading stage; Hainan is with to isolate breeding winter the selected rice root and stem of certain plants, individual plant sowing, go out rough, select wherein glutinous matter individual plant sowing, field planting performance breeding time and each economical character similar to former S system, namely be bred as and former S system only amylose content significant difference, the glutinous matter near-isogenic line photo-thermo-sensitive genic male sterile line of the equal no significant difference of other proterties wxs;
If c) one of this parent is restorer R, be transformed into glutinous matter parent, method is as follows: select high yield, high-quality, disease-resistant glutinous rice variety and this restorer R applied on producing to hybridize, obtain hybridization F 1seed; Sowing F 1with R system, make recurrent parent with R system, complete hybridization F heading stage 1with backcrossing of R system, obtain first backcross generation seed B 1f 1; By B 1f 1go out rough, select glutinous matter brown rice grain sowing wherein, sow R system simultaneously, select breeding time, individual plant that economical character is the most close with R system heading stage, complete B 1f 1backcross with the secondary of R system, obtain BC 1f 1seed; By BC 1f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows R system simultaneously, selects breeding time, individual plant that economical character is the most close with R system heading stage, completes BC 1f 1with backcrossing for three times of restorer, obtain BC 2f 1seed; By BC 2f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows R system simultaneously, selects breeding time, individual plant that economical character is the most close with R system heading stage, completes BC 2f 1with backcrossing for four times of R system, obtain BC 3f 1seed; By BC 3f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, BC 3f 1colony's field planting, each Agronomic neat and consistent, and similar to R system, be namely bred as and R system only amylose content significant difference, the glutinous matter near-isogenic line restorer of the equal no significant difference of other proterties wxr;
2) " non-glutinous male sterile line A or S/ glutinous matter near-isogenic line restorer is taked wxr, or glutinous matter near-isogenic line male sterile line wxa or glutinous matter near-isogenic line photo-thermo-sensitive genic male sterile line wxthe non-glutinous restorer R of S/ " Crossing system combo, obtain the Hybrid Rice Varieties that decreases than former hybrid rice of amylose content, F 1grain endosperm genotype is Wx wxwxor WxWx wx, F 2grain endosperm genotype is WxWxWx, WxWx wx, Wx wxwxwith wxwxwxfour kinds of mixed types.
3. the method for reduction hybrid rice rice grain amylose content according to claim 2, it is characterized in that: the concrete transformation process of step a) is as follows: select high yield, high-quality, disease-resistant glutinous rice variety and the maintainer B applied on producing to hybridize, obtain hybridization F 1seed; Sowing F 1with maintainer B, make recurrent parent with maintainer B, complete hybridization F heading stage 1with backcrossing of maintainer B, obtain first backcross generation B 1f 1seed; By B 1f 1go out rough, select glutinous matter brown rice grain sowing wherein, sow maintainer B simultaneously, select breeding time, individual plant that economical character is the most close with maintainer B heading stage, complete B 1f 1backcross with the secondary of maintainer B, obtain BC 1f 1seed; By BC 1f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows maintainer B simultaneously, selects breeding time, individual plant that economical character is the most close with maintainer B heading stage, completes BC 1f 1with backcrossing for three times of maintainer B, obtain BC 2f 1seed; By BC 2f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, sows maintainer B simultaneously, selects breeding time, individual plant that economical character is the most close with maintainer B heading stage, completes BC 2f 1with backcrossing for four times of maintainer B, obtain BC 3f 1seed; By BC 3f 1seed goes out rough, selects glutinous matter brown rice grain sowing wherein, BC 3f 1colony's field planting, each Agronomic neat and consistent, and similar to former B system, be namely bred as glutinous matter maintainer wxb; Then the sowing same period wxb and former A system, heading stage completes wxthe hybridization of B system and former A system, obtains hybridization F 1seed; Sowing F 1with wxb, heading stage completes F 1with wxb backcrosses, and obtains B 1f 1seed; By B 1f 1seed goes out rough, and select glutinous matter brown rice grain sowing wherein, the same period is sowed wxb, heading stage completes B 1f 1with wxb backcrosses, and obtains BC 1f 1seed; BC 1f 1waxy gene isozygotys, field planting, each Agronomic neat and consistent, and similar to former A system, is namely bred as glutinous matter near-isogenic line male sterile line wxa, continues to use wxb backcrosses and breeds this glutinous matter near-isogenic line male sterile line.
CN201410533003.1A 2014-10-11 2014-10-11 Method of reducing content of amylase in hybrid rice Pending CN104396723A (en)

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