CN110692513A - Method for cultivating indica-japonica hybrid rice variety with low amylose content - Google Patents
Method for cultivating indica-japonica hybrid rice variety with low amylose content Download PDFInfo
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Abstract
The invention discloses a method for cultivating indica-japonica hybrid rice varieties with low amylose content, which comprises the following steps: s1, breeding a glutinous rice sterile line; s2, breeding a partially indica japonica rice restorer; s3, hybridizing the sterile line of glutinous rice in the step S1 as female parent with the restorer line of indica type japonica rice in the step S2 to obtain F1(ii) a S4 and planting F1Thus obtaining the indica-japonica hybrid rice variety with low amylose content. The invention cultivates a new indica-japonica hybrid rice variety, the amylose content of the produced rice is kept about 10%, the technical difficulty of the quality deviation of the indica-japonica hybrid rice is solved, and the variety types of the hybrid rice are enriched; on the other hand, the invention can rapidly reduce the amylose content of rice by one-time seed production, omits the complicated steps of multi-generation selection after hybridization in the soft rice cultivation process in the prior art, can meet the market demand of rice, and makes certain contribution to the field of hybrid rice.
Description
Technical Field
The invention relates to the field of cultivation of new rice varieties, in particular to a method for cultivating indica-japonica hybrid rice varieties with low amylose content.
Background
The cooking and taste quality of rice refers to the characteristic characteristics of rice in the cooking and eating process, and is generally evaluated by several physical and chemical indexes such as amylose content, gelatinization temperature, gum consistency and the like.
The amylose content is the percentage of the amylose content in the polished rice and is an important grading index of the cooking taste quality of the rice. Japonica and indica varieties can be classified into four types, namely, extremely low (< 6%), low (6-15%), medium (15-25%) and high (> 25%), according to amylose content. With the improvement of living standard and the reduction of the amount of rice in the public, rice with 10% of amylose content is favored by more and more consumers because of soft taste and elasticity.
The amylose content is an important factor influencing the taste quality of rice, and researches show that the food taste quality of rice is influenced by the amylose content, the higher the amylose content is, the shorter the gum consistency is, the higher the gelatinization temperature is, the lower the disintegration value, the viscosity and the elasticity are, the harder the rice is, and the bad taste quality is. For example, the lower the amylose content, the shorter the consistency of the gum, the lower the gelatinization temperature, the higher the disintegration value, viscosity and elasticity, and the softer the rice. The amylose content of the edible rice is preferably 6 to 15 percent, and the edible rice has a good taste about 10 percent of amylose. The soft rice with 10% amylose content is soft but not sticky, has elasticity, good palatability and puffing property, is convenient to process, and is a first-class raw material for conditioning rice, puffed food and various rice snacks. Therefore, the soft rice has good market prospect and great potential.
However, the soft rice variety cultivated at present mainly comprises conventional rice, can be left in the seed, and is difficult to control; in addition, in the prior art, the soft rice cultivation process needs multi-generation selection after hybridization, and also needs continuous multi-generation tracking of amylose content, which wastes time and labor and can not meet the supply demand of the soft rice market.
Disclosure of Invention
The invention aims to solve the problems and provides a method for breeding indica-japonica hybrid rice with low amylose content, which is simple in breeding and easy in controlling the variety right.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a method for cultivating indica-japonica hybrid rice varieties with low amylose content comprises the following steps:
s1, breeding a glutinous rice sterile line;
s2, breeding a partially indica japonica rice restorer;
s3, hybridizing the sterile line of glutinous rice in the step S1 as female parent with the restorer line of indica type japonica rice in the step S2 to obtain F1；
S4 and planting F1Thus obtaining the indica-japonica hybrid rice variety with low amylose content.
Further, the amylose content of the sterile line of the glutinous rice in the step S1 is 1.5-2.5%.
Further, the sterile line of glutinous rice in step S1 is zhe glutinous 1A.
Further, the selecting and breeding of the sterile line of glutinous rice in the step S1 includes the following steps:
s11, crossing sterile cytoplasm donor 7627A with high-quality rice intermediate material ZP01-169 to obtain F1；
S12 selection of early-flowering sterility F1Backcrossing with intermediate material ZP01-169 to obtain BC1F1；
S13, sleeving self-crossing bags on the sterile line and the maintainer line during each backcross, harvesting sterile line backcross seeds with zero selfing setting rate and corresponding bag-sleeved maintainer line seeds, and ensuring the recovery and maintenance relationship of the next generation and the purity of the maintainer line;
s14, selecting sterile BC with early flowering, excellent rice quality and strong resistance1F1Backcrossing with intermediate material with ZP01-169 for 17 times to obtain sterile Zhenuo 1A.
Further, in the step S2, the amylose content of the partially indica japonica rice restorer line is 16.2 to 17.4%.
Further, the line name of the indica type japonica rice restorer line in the step S2 is zhe nong hui 1726.
Further, the selecting and breeding of the indica type indica rice restorer line in the step S2 includes the following steps:
s21, hybridizing the strain named Shaohui 093-1 with the strain named Zhenonghui 1012 to obtain F1；
S22, selecting F with big spike, thick stem and strong tillering1Performing sowing, planting and selfing to obtain F2；
S23, selecting F with vigorous growth, big spike and thick stalk2Performing sowing, planting and selfing to obtain F3Selfing is continuously planted for 5 times in the planting mode till F8And (4) shaping, namely, breeding the indica japonica rice restorer line Zhe nonghui 1726.
Compared with the prior art, the invention has the advantages and positive effects that:
according to the method for cultivating the indica-japonica hybrid rice variety with low amylose content, the glutinous rice sterile line Zhe glutinous 1A is selected as a female parent, and the partially indica japonica rice restorer line Zhe nonghui 1726 is selected as a male parent to be hybridized and matched, so that a new indica-japonica hybrid rice variety is cultivated, the variety of hybrid rice is enriched, the amylose content of the produced new indica-japonica hybrid rice variety is kept at about 10%, and the technical difficulty of the quality deviation of the indica-japonica hybrid rice is solved; on the other hand, the invention can rapidly reduce the amylose content of rice by one-time seed production, omits the complex steps of multi-generation selection after hybridization in the soft rice cultivation process and continuous multi-generation tracking of the amylose content in the prior art, can rapidly produce rice with low amylose content and good taste, meets the market demand of rice, and makes certain contribution to the field of hybrid rice.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a breeding process diagram of sterile line Zhe glutinous rice 1A of glutinous rice;
FIG. 2 is a process diagram of the breeding of restorer line Zhe nong Hui 1726 of indica type japonica rice;
FIG. 3 is a production flow chart of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived from the embodiments of the present invention by a person skilled in the art without any creative effort, should be included in the protection scope of the present invention.
As shown in fig. 1, 2 and 3, example 1, a method for breeding indica-japonica hybrid rice varieties with low amylose content, comprising the steps of:
s1, breeding a sterile line Zhe glutinous rice 1A of glutinous rice, wherein the amylose content is 1.5%;
s11, hybridizing the intermediate material with the variety name of 7627A and the code number of ZP01-169 to obtain F1；
S12, selecting early-flowering F1Backcrossing with intermediate material ZP01-169 to obtain BC1F1；
S13, selecting BC with early flowering, excellent rice quality and excellent resistance1F1And continuously backcrossing with an intermediate material with the code number of ZP01-169, and continuously backcrossing for 17 times in the planting way to obtain the sterile line Zhe glutinous 1A of the glutinous rice.
S2, breeding a restorer line Zhe nong hui of indica type japonica rice 1726, wherein the amylose content of the restorer line Zhe nong hui is 17.4%;
s21, hybridizing the strain named Shaohui 093-1 with the strain named Zhenonghui 1012 to obtain F1；
S22, selectingSelecting F with big ear, thick stem and strong tillering1Sowing and selfing to obtain F2；
S23, selecting F with vigorous growth, big spike and thick stalk2Sowing and selfing to obtain F3And the indica rice restorer line Zhe nong hui 1726 is obtained by planting and selfing for 5 times in the planting way.
S3, hybridizing the sterile line Zhe glutinous 1A of glutinous rice in the step S1 as female parent with the restorer line Zhe nonghui 1726 of indica type japonica rice in the step S2 to obtain F1Is named as Zhe Jing you 1726;
s4, planting Zhe Jing you 1726 to obtain the indica and japonica hybrid rice with low amylose content.
Embodiment 2, a method for breeding indica-japonica hybrid rice varieties with low amylose content, comprising the following steps:
s1, breeding a sterile line Zhe glutinous 1A of glutinous rice, wherein the amylose content is 2.2%;
s11, hybridizing the seeds with the variety name of 7627A with the intermediate material with the code number of ZP01-169 to obtain F1；
S12, selecting early-flowering F1Backcrossing with intermediate material ZP01-169 to obtain BC1F1；
S13, selecting BC with early flowering, excellent rice quality and excellent resistance1F1And continuously backcrossing with an intermediate material with the code number of ZP01-169, and continuously backcrossing for 17 times in the planting way to obtain the sterile line Zhe glutinous 1A of the glutinous rice. S2, breeding a restorer line Zhe nong hui of indica type japonica rice 1726, wherein the amylose content is 16.4%;
s21, hybridizing the strain named Shaohui 093-1 with the strain named Zhenonghui 1012 to obtain F1；
S22, selecting F with big spike, thick stem and strong tillering1Sowing and selfing to obtain F2；
S23, selecting F with vigorous growth, big spike and thick stalk2Sowing and selfing to obtain F3And the indica rice restorer line Zhe nong hui 1726 is obtained by planting and selfing for 5 times in the planting way.
S3, mixingHybridizing the sterile line Zhe glutinous 1A of glutinous rice in the step S1 as a female parent with the restorer line Zhe nonghui 726 of indica type japonica rice in the step S2 to obtain F1Is named as Zhe Jing you 1726;
s4, planting Zhe Jing you 1726 to obtain the indica and japonica hybrid rice with low amylose content.
Embodiment 3, a method for breeding indica-japonica hybrid rice varieties with low amylose content, comprising the following steps:
s1, breeding a sterile line Zhe glutinous 1A of glutinous rice, wherein the amylose content is 2.5%;
s11, hybridizing the intermediate material with the variety name of 7627A and the code number of ZP01-169 to obtain F1；
S12, selecting early-flowering F1Backcrossing with intermediate material ZP01-169 to obtain BC1F1；
S13, selecting BC with early flowering, excellent rice quality and excellent resistance1F1And continuously backcrossing with an intermediate material with the code number of ZP01-169, and continuously backcrossing for 17 times in the planting way to obtain the sterile line Zhe glutinous 1A of the glutinous rice.
S2, breeding a restorer line Zhe nong hui of indica type japonica rice 1726, wherein the amylose content is 16.2%;
s21, hybridizing the strain named Shaohui 093-1 with the strain named Zhenonghui 1012 to obtain F1；
S22, selecting F with big spike, thick stem and strong tillering1Sowing and selfing to obtain F2；
S23, selecting F with vigorous growth, big spike and thick stalk2Sowing and selfing to obtain F3And the indica rice restorer line Zhe nong hui 1726 is obtained by planting and selfing for 5 times in the planting way.
S3, hybridizing the sterile line Zhe glutinous 1A of glutinous rice in the step S1 as female parent with the restorer line Zhe nonghui 1726 of indica type japonica rice in the step S2 to obtain F1Is named as Zhe Jing you 1726;
s4, planting Zhe Jing you 1726 to obtain the indica and japonica hybrid rice with low amylose content.
After the seed is matureThe Zhe Jing you 1726 is harvested, the harvested rice is milled to obtain polished rice, 100 grains are randomly selected from the polished rice and are repeatedly detected for three times, and the detection result is as follows: 71.67 transparent round-grained nonglutinous rice grains and 28.33 opaque glutinous rice grains, which are tested by chi-square test2＝0.59<3.84, separation ratio of 3: 1; the amylose content of the harvested rice is measured, the amylose content is 9.6 percent, and the amylose content meets the following requirements of japonica in Zhe japonica you 1726 rice: amylose content of waxy-3: 1 mixed rice.
The sterile line Zhejiang glutinous rice 1A in the embodiment of the invention is identified by the crop variety approval committee of Zhejiang province in 12-month and 5-day 2011, and the identification number is as follows: zhe Yu Zhe Zi 2011008.
The Zhejiang glutinous rice 1A, Zhejiang nonghui 1726 and Zhejiang nonghui 1726 produced by hybridizing the Zhejiang glutinous rice 1A and the Zhejiang nonghui 1726 in the embodiment of the invention are sent to the detection center for rice quality inspection and detection in the ministry of agriculture for detection after more than 1 kg of seeds are harvested after maturation, the content of amylose in Zhejiang glutinous 1A is 1.5-2.5%, the content of amylose in Zhejiang nonghui 1726 is 16.2-17.4%, and the content of amylose in Zhejiang nonghui 1726 is 9.6-10.2%, so that the Zhejiang nonghui 1726 meets the.
According to the method for cultivating the indica-japonica hybrid rice variety with low amylose content, the glutinous rice sterile line Zhe glutinous 1A is selected as a female parent and the partially indica japonica rice restorer line Zhe nonghui 1726 are hybridized and matched, so that a new indica-japonica hybrid rice variety is cultivated, the variety of hybrid rice is enriched, the amylose content of the produced new indica-japonica hybrid rice variety is kept at about 10%, and the technical difficulty of the quality deviation of the indica-japonica hybrid rice is solved; on the other hand, the invention can rapidly reduce the amylose content of rice by one-time seed production, omits the complex steps of multi-generation selection after hybridization in the soft rice cultivation process and continuous multi-generation tracking of the amylose content in the prior art, can rapidly produce rice with low amylose content and good taste, meets the market demand of rice, and makes certain contribution to the field of hybrid rice.
Claims (7)
1. A method for cultivating indica-japonica hybrid rice varieties with low amylose content is characterized in that: the method comprises the following steps:
s1, breeding a glutinous rice sterile line;
s2, breeding a partially indica japonica rice restorer;
s3, hybridizing the sterile line of glutinous rice in the step S1 as female parent with the restorer line of indica type japonica rice in the step S2 to obtain F1；
S4 and planting F1Thus obtaining the indica-japonica hybrid rice variety with low amylose content.
2. The method of breeding indica-japonica hybrid rice varieties with low amylose content according to claim 1, wherein: in the step S1, the amylose content of the sterile line of the glutinous rice is 1.5-2.5%.
3. The method for breeding indica-japonica hybrid rice varieties with low amylose content according to claim 2, wherein the method comprises the following steps: the sterile line of the glutinous rice in the step S1 is Zhenuo 1A.
4. The method for breeding indica-japonica hybrid rice varieties with low amylose content according to claim 3, wherein the method comprises the following steps: the step S1 of selecting and breeding the sterile line of the glutinous rice comprises the following steps:
s11, crossing sterile cytoplasm donor 7627A with high-quality rice intermediate material ZP01-169 to obtain F1；
S12 selection of early-flowering sterility F1Backcrossing with intermediate material ZP01-169 to obtain BC1F1；
S13, sleeving self-crossing bags on the sterile line and the maintainer line during each backcross, harvesting sterile line backcross seeds with zero selfing setting rate and corresponding bag-sleeved maintainer line seeds, and ensuring the recovery and maintenance relationship of the next generation and the purity of the maintainer line;
s14, selecting sterile BC with early flowering, excellent rice quality and strong resistance1F1Backcrossing with intermediate material with ZP01-169 for 17 times to obtain sterile Zhenuo 1A.
5. The method of breeding indica-japonica hybrid rice varieties with low amylose content according to claim 4, wherein: in the step S2, the amylose content of the partially indica japonica rice restorer line is 16.2-17.4%.
6. The method of breeding indica-japonica hybrid rice varieties with low amylose content according to claim 5, wherein: the line name of the partially indica japonica rice restorer line in the step S2 is zhe nonghui 1726.
7. The method of breeding indica-japonica hybrid rice varieties with low amylose content according to claim 6, wherein: the selection and breeding of the indica-biased japonica rice restorer line in the step S2 comprises the following steps:
s21, hybridizing the strain named Shaohui 093-1 with the strain named Zhenonghui 1012 to obtain F1；
S22, selecting F with big spike, thick stem and strong tillering1Performing sowing, planting and selfing to obtain F2；
S23, selecting F with vigorous growth, big spike and thick stalk2Performing sowing, planting and selfing to obtain F3Selfing is continuously planted for 5 times in the planting mode till F8And (4) shaping, namely, breeding the indica japonica rice restorer line Zhe nonghui 1726.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114503909A (en) * | 2022-02-17 | 2022-05-17 | 江苏沿江地区农业科学研究所 | Method for directly producing polished round-grained glutinous rice formula rice by using special rice variety |
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| CN104396723A (en) * | 2014-10-11 | 2015-03-11 | 福建农林大学 | Method of reducing content of amylase in hybrid rice |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396723A (en) * | 2014-10-11 | 2015-03-11 | 福建农林大学 | Method of reducing content of amylase in hybrid rice |
Non-Patent Citations (2)
| Title |
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| 唐昌华等: "杂交稻浙糯优1号特征特性及栽培技术", 《浙江农业科学》 * |
| 徐伟东等: "水稻亚种间强优新组合生物学特性及利用价值", 《浙江农业学报》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114503909A (en) * | 2022-02-17 | 2022-05-17 | 江苏沿江地区农业科学研究所 | Method for directly producing polished round-grained glutinous rice formula rice by using special rice variety |
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Application publication date: 20200117 |