CN106332776A - Method for breeding high-quality disease-resistant wild-abortion cytoplasm male sterile rice - Google Patents
Method for breeding high-quality disease-resistant wild-abortion cytoplasm male sterile rice Download PDFInfo
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- CN106332776A CN106332776A CN201611008767.4A CN201611008767A CN106332776A CN 106332776 A CN106332776 A CN 106332776A CN 201611008767 A CN201611008767 A CN 201611008767A CN 106332776 A CN106332776 A CN 106332776A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for breeding high-quality disease-resistant wild-abortion cytoplasm male sterile rice. The method includes the steps that 1) conventional rice D and a existing maintainer line C are hybridized, Q2A serves as female parents, C*D F3-generation separation single plants serve as male parents, the Q2A and the C*D F3-generation separation single plants are hybridized respectively, and the hybridized products are expressed with A and B respectively; 2) in the proper season in Chongqi, the B is divided into two parts, and the two parts are planted in a rice-blast high incidence area and a high-temperature and low day-night temperature difference area respectively; 3) continuous screening, self crossing and back crossing of two growing seasons in Chongqi and self crossing and back crossing of two growing seasons in Hainan are carried out; 4) the A serves as female parents, a wild-abortion restoring line R1 and a wild-abortion restoring line R2 serve as male parents respectively, crossing testing combinations are prepared, and advantages are primarily tested; 5) in the third growing season in Chongqi, crossing testing combinations A*R1 and crossing testing combinations A*R2 are respectively divided into two parts which are planted in the rice-blast high incidence area and the high-temperature and low day-night temperature difference area respectively, and strains obtained after screening are the high-quality disease-resistant wild-abortion cytoplasm male sterile rice which is low in chalkiness ratio and high in transparency and head rice rate.
Description
Technical field
The invention belongs to agricultural breeding field, may be directly applied to " three line method " Parents In Hybrid Rice selection-breeding.
Background technology
Oryza sativa L. wild abortion type sterile series are wide due to its stable fertility, recovery spectrum, are currently Oryza sativa L. " three line method " hybrid vigor profit
Main path.But the universal rice blast resistance of hybrid rice variety being produced by Yebai CMS line in the market
Strong, rice matter not good it is impossible to meet the requirement of stable yields and high-quality.Thus, it is current for cultivating disease-resistant, high-quality hybrid rice variety
Main target.Hybrid paddy rice rice blast resistance is relevant with sterile line and restorer；Rice matter determines, it is by sterile line by grain endosperm
1 gamete of 2 gametes providing and restorer supply is developed.Therefore, compared to restorer, sterile line more can determine miscellaneous
Hand over the quality of rice.To improve disease resistance and the quality of hybrid rice variety simultaneously, cultivate that rice blast resistance is strong, that rice is of fine quality is sterile
System is crucial.In this context, we have carried out the breeding method exploration cultivating High quality and diseases resistance Yebai CMS line.
There is the defect of two aspects in existing Yebai CMS line, one is a meter matter difference, and prominent shows as chalkness ratio
Height, transparency and head rice rate are low, affect palatability and the commodity of hybrid rice variety；Two is that rice blast resistance is weak, gently then
Cause the Oryza sativa L. underproduction, heavy then have no harvest.
Content of the invention
For above-mentioned the shortcomings of the prior art, the invention provides a kind of High quality and diseases resistance Yebai three is not
Educate the selection being.
In order to solve above-mentioned technical problem, present invention employs following technical scheme:
A kind of selection of High quality and diseases resistance Yebai CMS line, the method comprises the steps:
1) use conventional Rice d and existing maintainer c hybridization, represented with c × d；The continuous selfing of filial generation two generation of c × d；From
Hand over to f3 for when, as female parent, in the f3 of c × d generation, separates individual plant and divides for male parent the Yebai backbone sterile line q2a being cultivated with our unit
Do not hybridize, introduce Cytoplasmic male sterile, realize sterile line and maintainer is supporting, represented with a and b respectively；
2) during the positive season of Chongqing, b is divided into two parts, is planted in rice blast district occurred frequently respectively and is unfavorable for high-quality trait expression
High temperature and low day-night temperature difference area；Meanwhile, high temperature and low day-night temperature difference area select economical character and the good b individual plant of flowering habit with
A is returned；After b yellow maturity, the appropriate seed collecting low altitude area b individual plant carries out Appearance Quality of Paddy Rice and amylose content inspection
Survey, investigate the rice blast incidence of rice blast district occurred frequently b simultaneously；After Chongqing Growing season terminates, in conjunction with every strain rice blast level
Number, chalky grain rate, head rice rate and amylose content, superseded rice blast comprehensive resistance be higher than 5 grades, chalky grain rate > 30%, whole
Polished rice rate<60%, the strain of amylose content<13% or>15%；
3) through selfing, the backcrossing of step sizing, selfing and the backcrossing of 2 Growing season in Chongqing and 2 Hainan Growing season,
A and b is basicly stable；
4) after a and b is basicly stable, made maternal with a, WA-CMS-type restorer r1 and r2 makees male parent respectively and prepare test cross combination
Carry out advantage preliminary survey, test cross group shares a × r1 and a × r2 and represents；
5) in the 3rd Chongqing Growing season, test cross is combined each point two parts of a × r1 and a × r2, plants occurred frequently in rice blast respectively
Area and the high temperature and the low day-night temperature difference area that are unfavorable for high-quality trait expression；After combination yellow maturity, to be combined in rice blast occurred frequently for investigation test cross group
The yield in area's rice blast incidence and high temperature and low day-night temperature difference area and rice matter；Eliminate yield poorly, resistance difference and rice matter difference b
Strain；Through screening the Yebai CMS line that remaining strain is exactly High quality and diseases resistance.
The invention has the beneficial effects as follows: can select that chalkness ratio is low using the inventive method, transparency and head rice rate
Height, and the Rice CMS-WA Type CMS line of energy blast resisting, are also had with the Hybrid Rice Combinations that this sterile line does maternal preparation
There is above-mentioned advantage, existing three lines paddy kind rice matter and the shortcoming of disease resistance difference can be improved, be conducive to Oryza sativa L. high quality and stable yield.
Brief description
Fig. 1 is a kind of flow chart of the selection of High quality and diseases resistance Yebai CMS line.
Specific embodiment
A kind of selection of High quality and diseases resistance Yebai CMS line, its flow process as shown in figure 1, the method include as follows
Step:
1) in order to improve existing maintainer (c represents) rice matter and disease resistance, with conventional Rice d and existing maintainer c hybridization, use
C × d represents；D does not contain the Restore gene of Yebai CMS line simultaneously with the feature that resistance and rice are of fine quality, c × d's
The continuous selfing of filial generation two generation；Selfing to f3 for when, with our unit cultivate Yebai backbone sterile line q2a as female parent, c ×
In the f3 generation of d, separates individual plant and hybridizes respectively for male parent, introduces Cytoplasmic male sterile, realizes sterile line and maintainer is supporting, point
Do not represented with a and b；
2) during the positive season of Chongqing, b is divided into two parts, is planted in rice blast district occurred frequently respectively and is unfavorable for high-quality trait expression
High temperature and low day-night temperature difference area (usually low altitude area)；Meanwhile, economical character and flowering habit are selected in low altitude area
Good b individual plant is returned with a；After b yellow maturity, the appropriate seed collecting low altitude area b individual plant carries out Appearance Quality of Paddy Rice and straight chain
Content of starch detects, investigates the rice blast incidence of rice blast district occurred frequently b simultaneously；After Chongqing Growing season terminates, in conjunction with every plant
It is rice blast series, chalky grain rate, head rice rate and amylose content, superseded rice blast comprehensive resistance is higher than 5 grades, the white grain of chalk
Rate>30%, head rice rate<60%, the strain of amylose content<13% or>15%；
3) through selfing, the backcrossing of step sizing, selfing and the backcrossing of 2 Growing season in Chongqing and 2 Hainan Growing season,
A and b is basicly stable；
4) after a and b is basicly stable, made maternal with a, WA-CMS-type restorer r1 and r2 makees male parent respectively and prepare test cross combination
Carry out advantage preliminary survey, test cross group shares a × r1 and a × r2 and represents；
5) in the 3rd Chongqing Growing season, test cross is combined each point two parts of a × r1 and a × r2, be planted in rice blast respectively high
Send out area and the high temperature and the low day-night temperature difference area that are unfavorable for high-quality trait expression；After combination yellow maturity, investigation test cross group is combined in rice blast height
Send out the rice blast incidence in area and be unfavorable for the high temperature of high-quality trait expression and the yield in low day-night temperature difference area and rice matter；Eliminate
Yield poorly, resistance difference and rice matter difference b strain；Through screening the Yebai CMS line that remaining strain is exactly High quality and diseases resistance.
Embodiment
Spring in 2008 existed in Hainan q2b (c) and Guangdong disease-resistant high-quality conventional Rice remaining boat silk Seedling (d) hybridization, in March, 2008
Chongqing plantation f1 generation (c × d), the autumn in 2008 Hainan plant f2 generation, the spring in 2009 Hainan f3 generation in select fine individual plant with
Q2a hybridizes, and introduces Cytoplasmic male sterile, and sterile line (a) and maintainer (b) are supporting.
2010 March in 2009 August selects the excellent of tool retentivity in 2, Chongqing Growing season and 2, Hainan Growing season
Good individual plant annual 3 season continuous backcross add generation.Wherein, maintainer strain is divided into two parts by 2 Growing season in Chongqing, plants respectively
Divide in Nanchuan of Chongqing (rice blast district occurred frequently) and Chongqing Ba Nan (being unfavorable for high temperature and the low day-night temperature difference area of high-quality trait expression)
Do not carry out rice blast screening and quality-screening.Superseded rice blast comprehensive resistance be higher than 5 grades, chalky grain rate>30%, head rice rate<
60%th, the strain of amylose content<13% or>15%.
Starting after autumn strain is stable within 2010 with Yebai three is Indica Rice Restorer Lines r28 (r1) and another name for Sichuan Province extensive 527 (r2) prepares
Cross combination carries out combining ability test.Test cross is combined (a × r1) and (a × r2) and is planted in Nanchuan of Chongqing respectively by March, 2011
Carry out rice blast, yield and quality screening with Chongqing Ba Nan.In conjunction with test cross result, obtain yield, exterior quality, rice blast resistance
The all more satisfactory stable strain with outcrossing habit, it is 9a that field is numbered.This sterile line has been returned 20 times at present, and maintainer adds generation
To f22 generation.
Finally illustrate, above example only in order to technical scheme to be described and unrestricted, although with reference to relatively
Good embodiment has been described in detail to the present invention, it will be understood by those within the art that, can be to the skill of the present invention
Art scheme is modified or equivalent, the objective without deviating from technical solution of the present invention and scope, and it all should be covered at this
In the middle of the right of invention.
Claims (1)
1. a kind of selection of High quality and diseases resistance Yebai CMS line is it is characterised in that the method comprises the steps:
1) use conventional Rice d and existing maintainer c hybridization, represented with c × d；The continuous selfing of filial generation two generation of c × d；Selfing is extremely
F3 for when, with self-fertile Yebai backbone sterile line q2a as female parent, in the f3 of c × d generation, separates individual plant and hybridizes respectively for male parent, introducing
Cytoplasmic male sterile, realizes sterile line and maintainer is supporting, is represented with a and b respectively；
2) during the positive season of Chongqing, b is divided into two parts, is planted in rice blast district occurred frequently and the high temperature being unfavorable for high-quality trait expression respectively
And low day-night temperature difference area；Meanwhile, select economical character in the high temperature being unfavorable for high-quality trait expression and low day-night temperature difference area and open
The good b individual plant of flower habit and a are returned；After b yellow maturity, the appropriate seed collecting low altitude area b individual plant carries out Appearance Quality of Paddy Rice
With amylose content detection, investigate the rice blast incidence of rice blast district occurred frequently b simultaneously；After Chongqing Growing season terminates, knot
Close every strain rice blast level, chalky grain rate, head rice rate and amylose content, superseded rice blast comprehensive resistance is higher than 5 grades, chalk
White grain rate>30%, head rice rate<60%, the strain of amylose content<13% or>15%；
3) through selfing, the backcrossing of step sizing, selfing and the backcrossing of 2 Growing season in Chongqing and 2 Hainan Growing season, a and b
Basicly stable；
4) after a and b is basicly stable, made maternal with a, WA-CMS-type restorer r1 and r2 makees male parent preparation test cross combination respectively and carry out
Advantage preliminary survey, test cross group shares a × r1 and a × r2 and represents；
5) in the 3rd Chongqing Growing season, test cross is combined each point two parts of a × r1 and a × r2, plant respectively in rice blast district occurred frequently and
It is unfavorable for high temperature and the low day-night temperature difference area of high-quality trait expression；After combination yellow maturity, investigation test cross group is combined in rice blast district occurred frequently
Rice blast incidence and be unfavorable for the high temperature of high-quality trait expression and the yield in low day-night temperature difference area and rice matter；Superseded yield
The b strain that low, resistance is poor and rice matter is poor；Through screening the Yebai CMS line that remaining strain is exactly High quality and diseases resistance.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611008767.4A CN106332776A (en) | 2016-11-16 | 2016-11-16 | Method for breeding high-quality disease-resistant wild-abortion cytoplasm male sterile rice |
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| CN201611008767.4A CN106332776A (en) | 2016-11-16 | 2016-11-16 | Method for breeding high-quality disease-resistant wild-abortion cytoplasm male sterile rice |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818457A (en) * | 2017-01-22 | 2017-06-13 | 福建农林大学 | A kind of selection of loose panicle type perfume rice CMS line |
| CN108235944A (en) * | 2017-05-08 | 2018-07-03 | 张亮 | A kind of indica Hybrid Rice CMS line breeding method |
| CN109006468A (en) * | 2018-10-16 | 2018-12-18 | 江西天涯种业有限公司 | A kind of Yebai three is the selection of indica CMS line rice |
Citations (2)
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| CN1659954A (en) * | 2004-02-27 | 2005-08-31 | 广东省农业科学院水稻研究所 | Method for making seeds through hybridizing among three series of paddy rice |
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| CN1659954A (en) * | 2004-02-27 | 2005-08-31 | 广东省农业科学院水稻研究所 | Method for making seeds through hybridizing among three series of paddy rice |
| CN105475121A (en) * | 2016-01-29 | 2016-04-13 | 重庆市农业科学院 | Breeding method for high-temperature-resistant and disease-resistant rice two-line sterile line |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818457A (en) * | 2017-01-22 | 2017-06-13 | 福建农林大学 | A kind of selection of loose panicle type perfume rice CMS line |
| CN106818457B (en) * | 2017-01-22 | 2018-10-23 | 福建农林大学 | A kind of selection of loose panicle type perfume (or spice) rice CMS line |
| CN108235944A (en) * | 2017-05-08 | 2018-07-03 | 张亮 | A kind of indica Hybrid Rice CMS line breeding method |
| CN109006468A (en) * | 2018-10-16 | 2018-12-18 | 江西天涯种业有限公司 | A kind of Yebai three is the selection of indica CMS line rice |
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Application publication date: 20170118 |