CN102550394B - Cold-region japonica hybrid rice restorer and variety breeding method - Google Patents
Cold-region japonica hybrid rice restorer and variety breeding method Download PDFInfo
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Abstract
The invention discloses a cold-region (I, II, III and IV rice accumulated temperature zone of Heilongjiang Province) japonica hybrid rice restorer and variety breeding method. Rice variety resources with cold-resistance restoring genes, namely restorer HP121 and restorer MR 18 are selected, and are planted and subjected to artificial cross, cold resistance is indentified at F3 stage after two continuous stages of system breeding, the screened cold-resistance lines are tested and matched with transformed male sterile lines and then are grown in the cold region F1, cold-resistance restorer is selected according to the representation of F1, and then male sterile lines are tested and matched with the restorer and are grown in the cold region F1, the effectiveness and the adaptability are indentified, and cold-region japonica hybrid rice restorer and variety can be obtained finally. According to the invention, the cold-resistant problem of the cold-region japonica hybrid rice restorer can be solved, three-system matching of the cold-region japonica hybrid rice is realized, the blank of applying japonica hybrid rice technology in the cold region of Heilongjiang Province can be filled, the output of cold-region rice can be greatly improved, and the application prospect is wide.
Description
Technical field
The present invention relates to the selection that a kind of cold ground japonica hybrid rice recovers system and kind.
Background technology
The Heilungkiang paddy rice occupies important status at present in China's grain-production.Heilungkiang belongs to typical cold ground rice workspace, and whole district rice made about 343.4 ten thousand hm of area in 2011
2, than 297.3hm in 2010
2Area increases about 46.6 ten thousand hm
2, and the trend of cumulative year after year is arranged.2011 annual output 6.07t/hm
2About, about about 2084.5 ten thousand t of gross output paddy, wherein 80% paddy is commodity food.The paddy rice that plantation is gone up in Heilungkiang production at present all is conventional variety, and unit yield maintains 6～7t/hm
2, the bottleneck that its output breaks through is the limitation of conventional variety.Use the successful experience of rice heterosis according to China south rice workspace, the Heilungkiang paddy rice also can be by the heterotic approach that utilizes, and significantly promotes output and the resistance of paddy rice on the kind, to satisfy market to the demand of Heilungkiang fine quality rice.This shows, present stage the hybrid rice technology to be applied to opportunity on cold ground ripe.
The hybrid rice technical research so far, because Heilungkiang exists objective condition such as year effective accumulated temperature is few, paddy growth crucial three periods (seedling stage, fringe phase, pustulation period) all easily meets with low temperature harm restriction coldly, cause and think always in the breeding research that the climatic environment of this area can not satisfy the needs of hybrid rice growth, so the research of cold ground hybrid rice up to now mostly rests on the technology exploration stage.Look into new result and show, still do not have recovery system (R), the male sterile line (A) of cold ground of the adaptation rice workspace cultivation of breeding at present, do not realize that more the hybrid rice variety of three series mating emerges.
Realize the hybrid rice technology in the application on cold ground, key is to solve the cold resistance problem of recovering system.
Summary of the invention
The purpose of this invention is to provide the selection that a kind of cold ground japonica hybrid rice recovers system and kind, realize cold ground japonica hybrid rice three series mating.
Technical scheme of the present invention is as follows:
Cold ground japonica hybrid rice recovers the selection of system and kind, it is characterized in that may further comprise the steps:
(1) screening has the variety resources of rice that cold resistance is recovered gene, and selecting the Java type to recover is that HP121 (it is extensive No. 9 of 2//Anhui that Chinese PL/ overcharges), recovery are MR18 (LH422 surveys roguing system);
(2) to recover be HP121 to plantation Java type, to recover be the MR18 variety source, and according to HP121/ // close positive 23/MR18//MR18 artificial hybridization, through continuous 2 generation systematic breeding, the screening economical character meets the fine individual plant of planting coldly, at F
3Identify its cold resistance;
(3) three is male sterile line northern part of the country 1A, backcrosses 4 times to 4 generation (BC after liking to know fragrant A and cultivating rice 12 testcross and screenings
4) transformation obtains; Northern part of the country 2A backcrosses 4 times to 4 generation (BC after liking to know fragrant A and Sha Sha mud testcross and screening
4) transformation obtains;
(4) be 5 generation (F with male sterile line northern part of the country 1A, the northern part of the country 2A of transformation and the cold resistance strain of step (2) seed selection
5) survey extensive combo, planting F coldly
1, the recovery that the screening acquisition has cold resistance is 7 generation (F
7), repeated test is to 8 generation (F
8) ground japonica hybrid rice recovery system obtains to tremble with fear;
(5) with the male sterile line combo of above-mentioned recovery system and transformation, cold ground of seed selection japonica hybrid rice kind.
Described cold ground japonica hybrid rice recovers the selection of system and kind, it is characterized in that described cold ground, refers in particular to China I of Heilongjiang Province, II, III, the IV rice makes the accumulated temperature band and corresponding rice does the ecotope.
Described cold ground japonica hybrid rice recovers the selection of system and kind, it is characterized in that described cold ground japonica hybrid rice recovers system and is numbered: JR10004, JR10012, JR10036 series; Described cold ground japonica hybrid rice kind is: recovering with male sterile line northern part of the country 1A, northern part of the country 2A and JR series is the cold ground japonica hybrid rice combination of combo.
Described cold ground japonica hybrid rice recovers the selection of system and kind, it is characterized in that cold ground japonica hybrid rice recover system as the hybrid strain seed selection similarly cold ground japonica hybrid rice recover system and kind.
The present invention at first seed selection has the japonica hybrid rice recovery system that cold resistance is recovered gene, simultaneously under Yuan Longping academician's's " when the river rises the boat goes up " hybrid rice theoretical direction, utilize local conventional rice resource success transformation male sterile line, then realize three series mating by surveying extensive combo, bred the hybrid rice new varieties that adapt to the different accumulated temperature bands in Heilungkiang.The present invention has cold resistance and recovers the selection that the cold ground japonica hybrid rice of gene recovers system and kind, has all filled up the historical blank in this field at aspects such as core technology, resource material, Hybrids.Can increase substantially paddy rice in cold region output, application prospect is very wide.
Embodiment
Example 1
Parental source
Recovery is HP121: it is that the paddy rice Java type recovery that extensive No. 9 selection cross in 2//Anhui form is HP121 that academy of agricultural sciences, Anhui Province paddy rice research institute overcharged by Chinese PL1/ in 1994.
Recovery is MR18: Xiuqian City Feng He paddy rice research institute was that LH422 surveys roguing system (LH422 is provided by assorted excellent center Mr. Luo Xiaohe in Hunan) from recovering in 1999.
Cultivate rice 12: cultivating rice 12 is that Heilongjiang Province paddy rice research institute of the land-reclaimable academy of sciences is for No. 10 female parent to cultivate rice, is for No. 8 paternal hybrid to cultivate rice, and the pedigree method seed selection forms.Authorization is numbered the black rice 2006009 of examining.Market is bought.
Sha Sha mud: Fangzheng County, Heilongjiang Province seeds company, Suihua Inst. of Agricultural Science, Heilongjiang Prov. Academy of Agricultural are adapted at Heilongjiang Province's second accumulated temperature band plantation from the conventional japonica rice kind of external introducing.Authorization is numbered the black rice 2006011 of examining.Market is bought.
Like to know fragrant A: fragrant and BT type male sterile line 6,000 hot A test crosses are liked to know with Japanese improved seeds by Xiuqian City Feng He paddy rice research institute 1999, and backcross transformation forms, and authorization being numbered Soviet Union and examining rice: 200313.
Process of the test
1, in August, 2006 is in the Xuancheng, Anhui
Recovering with the Java type is that HP121 makes maternal (the offspring F of close positive 23/MR18//MR18
6) be paternal hybrid.
2, in March, 2007 in the Sanya, in August, 2007 is in the Xuancheng, Anhui
Through continuous 2 generation the systematic breeding economical character meet the fine individual plant of planting coldly.
3, in December, 2007～2008 year March is in Sanya, Hainan
Plantation F
3, utilize the low condition (table 1) of Hainan Island mean temperature in February: (1) filters out cold resistance strain system according to ripening rate from strain system, and makes up the F of the suitable cold resistance strain system of planting of economical character coldly
4Inbred line population; (2) cultivate rice 12, Sha Sha mud and respectively select dissimilar individual plants from the conventional rice of plantation simultaneously, like to know that fragrant A carries out test cross with it with BT type Japonica Hybrid male sterile line, structure male sterile line transformation colony.
4, in August, 2008 is respectively in 5 constant virtues, Heilungkiang and Funing County, Jiangsu
5 constant virtues, Heilungkiang: the suitable F that plants of selected economical character from the inbred line population that the cold resistance strain of planting is coldly
5Strain system.
Funing County, Jiangsu: know fragrant A and the test cross F that cultivates rice 12, the different individual plants of Sha Sha mud from the love of plantation
1Select in the colony individual plant all sterile, microscopy dye and lose (black dying) pollen rate and carry out individual plant again with the test cross male parent and backcross, and structure is cultivated the BC of rice 12 and 2 types of Sha Sha mud in the system of the strain 5% below
1Backcross transformation colony.
5, in December, 2008～2009 year March is in Sanya, Hainan
(1) plantation cold resistance inbred line population; (2) BC of rice 12 and 2 types of Sha Sha mud is cultivated in plantation
1Backcross transformation colony is from BC
1In select individual plant all sterile, microscopy dye and lose (black dying) pollen rate 5% below and the backcross sterile strain of male parent of economical character trend, backcross with corresponding male parent individual plant, and structure is cultivated the BC of rice 12 and 2 types of Sha Sha mud
2Backcross transformation colony; (3) respectively with the BC that cultivates rice 12 and 2 types of Sha Sha mud
1Backcross sterile strain and the cold resistance strain of male parent of economical character trend is F in the colony
5The inbred line individual plant is surveyed extensive combo F
1
6, in August, 2009 is in 5 constant virtues, Heilungkiang and Hefei ,Anhui
5 constant virtues, Heilungkiang: the F of extensive combo is surveyed in (1) plantation
1, utilize paddy rice booting on 15～September 15 in July then, heading, condition (table 2) that the pustulation period temperature is on the low side, 29 F that survey extensive combos
1Middle ripening rate reaches have only 3 more than 75%, and ripening rate reaches have only 1 more than 85%, according to F
1The low temperature ripening rate filters out the corresponding JR09095 of being numbered, JR09161, the JR09169 cold resistance is recovered system's (table 3); (2) plantation cold resistance inbred line, according to low temperature ripening rate The selection result, having made up the cold resistance recovery is F
6Inbred line population.
The Hefei ,Anhui: the BC of rice 12 and 2 types of Sha Sha mud is cultivated in plantation
2Backcross transformation colony is from BC
2In select individual plant all sterile, microscopy dye and lose (black dying) pollen rate 5% below and the backcross sterile strain of male parent of economical character trend, backcross with corresponding male parent individual plant, and structure is cultivated the BC of rice 12 and 2 types of Sha Sha mud
3Backcross transformation colony.
7, year Sanya, Hainan in March, in December, 2009～2010
(1) recovery of plantation cold resistance is inbreeding population; (2) BC of rice 12 and 2 types of Sha Sha mud is cultivated in plantation
3Transformation colony is from BC
3In select individual plant all sterile, microscopy dye and lose (black dying) pollen rate 5% below and the backcross sterile strain of male parent of economical character trend, carries out individual plant again with male parent and backcross, and structure is cultivated the BC of rice 12 and 2 types of Sha Sha mud
4Backcross transformation colony; (3) respectively with the BC that cultivates rice 12 and 2 types of Sha Sha mud
3The backcross sterile strain of male parent of economical character trend is F with the cold resistance recovery in the colony
7The inbred line individual plant is surveyed extensive combo F
1
8, in August, 2010 is in Hefei ,Anhui and Jiamusi, Heilungkiang
The Hefei ,Anhui: the BC of rice 12 and 2 types of Sha Sha mud is cultivated in plantation
4Transformation colony, BC
4Performance complete sterility, economical character do not have separation and consistent with the male parent of backcrossing, with BC
4It is numerous to mix system's expansion, will cultivate the BC of rice 12 types
4Name the BC for northern part of the country 1A, Sha Sha mud type
4Name and be northern part of the country 2A.
Jiamusi, Heilungkiang: extensive combo F is surveyed in plantation
1, from 78 test cross F
1In selected 4 combination ripening rates reach 80% or more, moderate growth duration, tillering ability and grain number per spike have clear superiority kind (combination) northern part of the country 2A/JR10004, northern part of the country 1A/JR10012, northern part of the country 2A/JR10012, northern part of the country 2A/JR10036, and corresponding cold resistance recovery is (F
8) be numbered series such as JR10004, JR10012, JR10036 (table 4).
Sanya, table 1 Hainan in 2008 daily temperature data in February 15～29
Annotate: according to weather forecast record at that time.
5 constant virtues, table 2 Heilungkiang in 2009 daily temperature data in 15～September of July 15
Annotate: the data that provides according to the general meteorological station of 5 constant virtues country.
Table 3 japonica hybrid rice F1 in 2009 Other Main Agronomic Characters is investigated the result
Annotate: April 20 sowing time, transplant May 27 phase, plant specification 26.4cm * 16.5cm, every cave 1 seedling.
Table 4 japonica hybrid rice F1 in 2010 Other Main Agronomic Characters is investigated the result
Annotate: April 20 sowing time, transplant May 25 phase, plant specification 26.4cm * 16.5cm, every cave 1 seedling.
Example 2
The seed selection process;
1, sterile line breeding: northern part of the country 1A, northern part of the country 2A backcrossed 4 times to 4 generation (BC respectively in 2008～2010 years after liking to know fragrant A and cultivating rice 12, Sha Sha mud testcross and screening
4) transformation obtains.
2, to recover be seed selection to cold ground japonica hybrid rice: in 2006～2010 years with Java type HP121 (it is extensive No. 9 of 2//Anhui that Chinese PL/ overcharges) do maternal, make male parent with " close positive 23 make maternal and MR18 does the offspring that paternal hybrid is backcrossed with MR18 again ", carry out artificial hybridization, through continuous 2 generation systematic breeding, the screening economical character meets the fine individual plant of planting coldly, at 3 generation (F
3) identify its cold resistance; The plantation screening is to 5 generation (F
5), survey after the extensive combo planting coldly with male sterile line northern part of the country 1A, the northern part of the country 2A of transformation and to survey the F that joins
1, according to F
1Recovery with cold resistance of ripening rate screening be 7 generation (F
7), repeated test is to 8 generation (F
8) ground japonica hybrid rice recovery system obtains to tremble with fear.
3, hybrid rice variety seed selection: with the male sterile line combo of above-mentioned recovery system and transformation, cold ground of seed selection japonica hybrid rice kind.
Claims (4)
1. cold ground japonica hybrid rice recovers the selection of system and kind, it is characterized in that may further comprise the steps:
(1) screening has the variety resources of rice that cold resistance is recovered gene, and selecting the Java type to recover is that HP121, recovery are MR18;
(2) to recover be HP121 to plantation Java type, to recover be the MR18 variety source, and according to HP121/ // close positive 23/MR18//MR18 artificial hybridization, through continuous 2 generation systematic breeding, the screening economical character meets the fine individual plant of planting coldly, at F
3Identify its cold resistance;
(3) three is male sterile line northern part of the country 1A, backcrosses 4 times to 4 generation (BC after liking to know fragrant A and cultivating rice 12 testcross and screenings
4) transformation obtains; Northern part of the country 2A backcrosses 4 times to 4 generation (BC after liking to know fragrant A and Sha Sha mud testcross and screening
4) transformation obtains;
(4) be 5 generation (F with male sterile line northern part of the country 1A, the northern part of the country 2A of transformation and the cold resistance strain of step (2) seed selection
5) survey extensive combo, planting F coldly
1, the recovery that screening has cold resistance is 7 generation (F
7), repeated test is to 8 generation (F
8) ground japonica hybrid rice recovery system obtains to tremble with fear;
(5) with the male sterile line combo of above-mentioned recovery system and transformation, cold ground of seed selection japonica hybrid rice kind.
2. cold ground as claimed in claim 1 japonica hybrid rice recovers the selection of system and kind, it is characterized in that described cold ground, refers in particular to China's Heilongjiang Province's I, II, III, the IV rice makes the accumulated temperature band and corresponding rice does the ecotope.
3. the selection of cold ground japonica hybrid rice recovery system as claimed in claim 1 and kind is characterized in that described cold ground japonica hybrid rice recovers system and is numbered: JR10004, JR10012, JR10036 series; Described cold ground japonica hybrid rice kind is: recovering with male sterile line northern part of the country 1A, northern part of the country 2A and JR series is the cold ground japonica hybrid rice combination of combo.
4. the described cold ground of claim 1 japonica hybrid rice recovers the selection of system and kind, it is characterized in that cold ground japonica hybrid rice recover system as the hybrid strain seed selection similarly cold ground japonica hybrid rice recover system and kind.
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| CN104429750B (en) * | 2013-09-18 | 2017-02-15 | 中国农业大学 | Method used for identifying cold tolerance of rice at booting stage |
| CN106332770A (en) * | 2015-12-29 | 2017-01-18 | 湖南杂交水稻研究中心 | Cold region hybrid japonica rice breeding method |
| CN112586342B (en) * | 2020-12-04 | 2022-03-01 | 国家粳稻工程技术研究中心 | Breeding method for improving cold resistance of japonica rice of restorer line |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1411701A (en) * | 2002-11-21 | 2003-04-23 | 江西省农业科学院水稻研究所 | Screening method for breeding strong cold-resisting new rice variety |
| CN1939122A (en) * | 2005-09-28 | 2007-04-04 | 袁宏伟 | Cultivation of excellent rice with huge plumule in cold temperate zone |
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2011
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1411701A (en) * | 2002-11-21 | 2003-04-23 | 江西省农业科学院水稻研究所 | Screening method for breeding strong cold-resisting new rice variety |
| CN1939122A (en) * | 2005-09-28 | 2007-04-04 | 袁宏伟 | Cultivation of excellent rice with huge plumule in cold temperate zone |
Non-Patent Citations (4)
| Title |
|---|
| 张培江等.水稻爪哇型恢复系HP121 的选育和应用.《安徽农业科学》.1998,第26卷(第2期),第98-100页. |
| 李永祥等.黑龙江寒地杂交水稻育种研究初报.《现代农业科技》.2012,(第7期),第84，88页. |
| 水稻爪哇型恢复系HP121 的选育和应用;张培江等;《安徽农业科学》;19981231;第26卷(第2期);第98-100页 * |
| 黑龙江寒地杂交水稻育种研究初报;李永祥等;《现代农业科技》;20121231(第7期);第84，88页 * |
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