CN106818457B - A kind of selection of loose panicle type perfume (or spice) rice CMS line - Google Patents
A kind of selection of loose panicle type perfume (or spice) rice CMS line Download PDFInfo
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- CN106818457B CN106818457B CN201710047684.4A CN201710047684A CN106818457B CN 106818457 B CN106818457 B CN 106818457B CN 201710047684 A CN201710047684 A CN 201710047684A CN 106818457 B CN106818457 B CN 106818457B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of selections of loose panicle type perfume (or spice) rice CMS line, belong to Special rice sterile line breeding technical field.The present invention is with loose panicle type rice strain, fragrant rice three is maintainer, CMS line, three be that ripe restorer is hybrid strain late for Poa linn and three systems, using hybridization and mostly for the method for backcross transformation, and fringe type, fragrance, identification of male sterility through mostly generation, Comprehensive Traits screening, be bred as loose panicle type, rice aroma are strong, sterility is stable, coordinate force is good, breeding, the production of hybrid seeds and the high excellent loose panicle perfume (or spice) rice CMS line of hybrid yield.The hybrid seed outcrossing seed-setting rate that the sterile line is prepared is high, and seed procreation ability is strong, and rice appearance and Cooking Quality are good, and the fringe portions pest and disease damage such as false smut is substantially reduced, can reduce the usage amount of pesticide, increase farmers' income.
Description
Technical field
The invention belongs to Special rice sterile line breeding technical fields, and in particular to a kind of loose panicle type perfume (or spice) rice CMS line
Selection.
Background technology
Breeding Rice for Ideotype is an important directions of breeding in recent years, and one of Ideotype Breeding research focus
It is exactly fringe type problem.Fringe type is one of important morphological feature of rice, and fringe type and rice yield level and group structure situation are equal
There is close relationship, thus fringe type problem is always the focus of Breeding Rice for Ideotype and cultivation research.According to point of fringe
Branch pattern, the dense degree and spatial attitude of the angle of Primary branch and small ear, can be divided into intensity by fringe type（Compact
panicle）, scatter type（Loose panicle type）（Spreading panicle）And osculant（Intermediate panicle）.By
Currently, reporting 7 loose panicle character genes altogether, wherein spr1 and spr8 is controlled by 1 recessive nuclear gene, remaining 5 loose panicle
Character gene is controlled by dominant gene.Studies have shown that the fringe type of rice in claw-like be conducive to increase fringe portion Primary branch between
Space, fringe portion branch obstructs the increase of spreading angle, increases that illumination is penetrating and grain filling characteristics, reduces the white grain of chalk of rice
Rate and total chalkiness degree, green flat seed significantly reduce, and improve exterior quality；Meanwhile loose panicle type branch obstructs that the male character types in Chinese operas, usu. referring tov the bearded character degree is big, fringe shape phase
To it is loose, improve fringe portion miniclimate, the pest and disease damages such as fringe portion false smut are substantially reduced.Selection and breeding loose panicle type Three-line rice sterile line can
Go out the new hybrid rice varieties of high yield and high quality with combo.
Scented rice rice is a kind of high-quality seed rice with tempting special fragrance.Its type includes that perfume Xian, fragrant round-grained rice and perfume (or spice) are glutinous, is had
There is special fragrance, the grain of rice is sparkling and crystal-clear, is rich in a variety of necessary nutritional ingredients of human body, has certain nourishing and medicinal effects.Its fragrance
Can disappear removing fatigue, and help to improve a poor appetite.In addition to it can make rice, various rice wine can be also produced, the food such as rice cake, taste is more
It is good." the ivory scented rice " of Yunnan Province of China, " eight treasures (choice ingredients of certain special dishes) fragrant ", " the fragrant standing grain " in Guizhou, Guangdong " Wan Jiaxiang ", have that " one is cooked ten thousand
The laudatory title of perfume ".Hunan Research Centre for Hybrid Rice is bred as first, China aromatic indica CMS line " new perfume A ", the recovery with non-fragrance
" bright extensive 77 " hybridize allocation to rent, are bred as hybrid paddy rice " new fragrant excellent 77 " of meter Zhi Da two levels for system.But the domestic fragrant rice strain being bred as at present
Fringe type be intensity, there is no loose panicle type scented rice sterile line be bred as report.
It is parent that this method, which chooses loose panicle type strain and fragrant rice maintainer, using hybridization Backcrossing methods, by loose panicle gene with
Scent gene is aggregated in same maintainer, then sterile by being bred as loose panicle, the fragrant rice of aromatic flavour with sterile line backcross transformation
System.The selection is efficient, profitable, and the sterile line coordinate force of incubation is strong, and the hybrid yield of combo is high, rice is of fine quality, nutrition
The fringe portions pest and disease damages such as good, anti-false smut.
Invention content
The purpose of the present invention is to provide a kind of selections of loose panicle type perfume (or spice) rice CMS line.
The present invention is with loose panicle type rice strain, and fragrant rice three is maintainer, and CMS line, three be Poa linn and three systems
Slow ripe restorer is hybrid strain, is returned by hybridization and mostly generation, and the identification of fringe type and fragrance through mostly generation, sterile line and holding
It is objective trait screening, is bred as loose panicle, the excellent perfume (or spice) that rice aroma is strong, sterility is good, hybrid seed yield is high and hybrid yield is high
Rice CMS line.
To achieve the above objectives, the present invention uses following technical scheme：
1）Juvenile stage：It is hybrid strain P1 to select the rice strain of loose panicle type, and fragrant rice three is that maintainer is P2, and three systems are not
It is for P3 to educate, and three be Poa linn be P4, and ripe restorer is P5 late for three systems；
2）Hybridize combo and screening technique：
a）Make hybridization P1/P2, F must be hybridized1Seed, with F1- 12 indicate；
b）Plant F1- 12 15-25 plants of plant, the maturity period eliminates pseudostationary, mixes and receives true hybrid, obtains F2For seed, with F2-12
It indicates；
c）Plant F2- 12 3500-4000 plants of plant；Heading stage carries out fragrance mirror to the blade for the loose panicle single plant isolated
It is fixed, listing mark；Maturity period, sowing, obtained F respectively to having the single plant of loose panicle and fragrance concurrently3For seed, with F3- 12 indicate；
d）Strain sows F3- 12 seeds, each strain plant 35-40 plants, and full heading time is dissipated to what each strain was isolated
The blade of fringe strain carries out fragrance identification, and single plant and strain listing mark to having loose panicle and fragrance concurrently；Maturity period is scattered to having concurrently
The single plant of fringe and fragrance is that the objective trait that maintainer should have carries out evaluation and screening by three, and objective trait includes：Full fertility
80-125 days phase, 65-85 centimetres of plant height, 20-30 centimetres of spike length, grain number per spike 90-140, spike number 12-18, grain length-width ratio
2.5-4.5,20-30 grams of mass of 1000 kernel, setting percentage 85%-95%；250-300 plants of single plant sowing, obtains F4For seed, with F4- 12 indicate；
e）Strain sows F4- 12 seeds and CMS line P3, each strain plant 30-35 plants, P3 points of CMS line
Three phases were sowed；Full heading time selects the blade of 5 single plants to carry out fragrance identification, and right to not detaching strain with loose panicle character
The strain for having loose panicle and fragrance concurrently is that the objective trait that maintainer should have carries out observation identification by three, selects strain 80-90
Card mark is inserted, each slotting board strain selects 5 plants to carry out pairs of test cross with P3；Maturity period selects have target in slotting card mark strain
150-200 plants of listing marks of single plant of character, be listed strain single plant sowing；F4Brown rice fragrance mirror is carried out after the shelling of -12 strain seeds
It is fixed, British plain spirits single plant is removed, retains and has dulcet single-strain seed；Pairs of test cross marks sowing；Obtain F5For seed, with F5- 12 tables
Show, and test cross 1 generation seed in pairs, with F1- 123 indicate；
f）Strain sows F5- 12 and F1- 123 seeds, each strain plant 30-35 plants, and full heading time is to loose panicle
Shape does not detach strain, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is kept by three systems
It is the objective trait that should have, selects 20-30 slotting card marks of strain；The sterile plant of pairs of test cross ties up to full heading time observation
Flowering habit is concentrated when selection is spent, opens the strain progress pollen sterility degree that the clever time is long, pollen loose powder is good, stigma exposing ratio is high
Microscopy retains single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；Listed strain F5-
12 and F1- 123 mark sowing in pairs；F5Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits single plant, is retained
Have dulcet single-strain seed, obtains F6For seed, with F6- 12 indicate, and 1 generation seed of backcrossing, with BC1F1- 123 indicate；
g）Strain sows F6- 12 and BC1F1- 123 seeds, each strain plant 30-35 plants, and full heading time is scattered to having
Fringe character does not detach strain, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, by three systems
The objective trait that maintainer should have selects 20-30 slotting card marks of strain；The sterile plant of pairing backcrossing ties up to full heading time
Flowering habit is observed, concentrated when selection is spent, open clever time length, the strain that pollen loose powder is good, stigma exposing ratio is high carries out pollen not
Degree of educating microscopy retains single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；It is listed
Strain F6- 12 and BC1F1- 123 mark sowing in pairs；F6Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits
Single plant retains and has dulcet single-strain seed, obtains F7For seed, with F7- 12 indicate, and 2 generation seeds of backcrossing, with BC2F1-123
It indicates；
h）Strain sows F7- 12 and BC2F1- 123 seeds, each strain plant 30-35 plants, and full heading time is scattered to having
Fringe character does not detach strain, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, by three systems
The objective trait that maintainer should have selects 20-30 slotting card marks of strain；The sterile plant of pairing backcrossing ties up to full heading time
Flowering habit is observed, concentrated when selection is spent, open clever time length, the strain that pollen loose powder is good, stigma exposing ratio is high carries out pollen not
Degree of educating microscopy retains single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；It is listed
Strain F7- 12 and BC2F1- 123 mark sowing in pairs；F7Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits
Single plant retains and has dulcet single-strain seed, obtains F8For seed, with F8- 12 indicate, and 3 generation seeds of backcrossing, with BC3F1-123
It indicates；
i）Continue step h) until obtaining F10- 12 and BC5F1- 123 seeds；Strain sows F10- 12 and BC5F1- 123 seeds, with
And three be Poa linn P4 and three systems ripe restorer P5 late, each strain plants 30-35 plants；Continue backcross transformation, obtains BC6F1-
123 seeds；Using P4 and P5 to BC5F1- 123 carry out test cross, with the breeding time feature and restorability of the sterile strain of identification, obtain
Test cross F1- 1 and F1- 2 seeds；Sowing F10- 12 strains, obtain F11- 12 seeds；
j）Strain sows F11- 12 and BC6F1- 123 seeds and test cross F1-1、F1- 2 each 30；To 30 test cross F1-1
And F1- 2 carry out Yield comparison experiment, are filtered out respectively with the excellent F of loose panicle, aromatic flavour, Comprehensive Traits1-1、F1- 2 groups
It closes, corresponding F11- 12 strains and BC6F1- 123 strains are the maintainer strain being selected in and sterile line strain, that is, have been bred as dissipating
Fringe type perfume (or spice) rice CMS line.
Wherein, the loose panicle type strain（P1）Forspr8（Chen Pingping, Ye Shenghai, Lu Yanting, gold are celebrated one's birthday, Zhang little Ming
Feature and its mutator the positioning nuclear agricultural science reports of 22 loose panicle mutant spr8 of late japonica rice Zhejiang round-grained rice, 2013,27,1-8.
）Orsp（The genetic analysis of Liu Dan, Wang Jiayu, Liu Jin, Ma Dianrong, Zhao Minghui, Chen Wenfu rice bulk fringe mutant sp
And gene just positions Botany Gazettes, 2015,50 (2): 198–205）, fragrant rice three is maintainer（P2）For Lu perfume (or spice) 618B, three
It is sterile line（P3）For the anti-A of gold, three be Poa linn（P4）For Minghui 2155, three systems ripe restorer late（P5）For another name for Sichuan Province extensive 527.
Beneficial effects of the present invention：
Effect of the invention is that hybridization Backcrossing methods are respectively adopted, loose panicle gene and scent gene are aggregated to same guarantor
In holding and being, then pass through backcross transformation be bred as loose panicle, aromatic flavour fragrant rice CMS line.The selection is efficient, benefit
Good, the sterile line coordinate force of incubation is strong, the fringe portions pest and disease damage such as hybrid yield high, rice is of fine quality, nutrition is good, anti-false smut of combo.
The fragrant rice hybridization rice combination for planting sterile line preparation, can reduce the usage amount of pesticide, reduce recruitment, reduce environmental pollution, increase
Add farmers' income.The perfume rice hybridization rice varieties are planted, increase yield 4-7% than common rice kind per acre, reduce dispenser cost
60-90 members, scented rice paddy purchasing price increase 20-22%, can be that peasant increases economic benefit 320-400 members per acre, reduce simultaneously
Pollution of the pesticide to environment.
Specific implementation mode
Embodiment 1：
1）Select the rice strain of loose panicle typespr8（P1）, it is maintainer Lu perfume (or spice) 618B to have fragrance three（P2）, three systems infertility
The anti-A of system's gold（P3）, three be Poa linn Minghui 2155（P4）With three systems ripe restorer another name for Sichuan Province extensive 527 late（P5）For hybrid strain；
2）Make hybridization P1/P2, F must be hybridized1Seed, with F1- 12 indicate；
3）Plant F1- 12 15 plants of plant, the maturity period eliminates pseudostationary, mixes and receives true hybrid, obtains F2For seed, with F2- 12 tables
Show；
4）Plant F2- 12 3840 plants of plant.Heading stage carries out fragrance identification to the blade for the loose panicle single plant isolated, and hangs
Card mark；Maturity period, sowing, obtained F respectively to having the single plant of loose panicle and fragrance concurrently3For seed, with F3- 12 indicate；
5）Strain sows F3- 12 seeds, each strain plant 35 plants, the loose panicle strain that full heading time isolates each strain
Blade carry out fragrance identification, and single plant and strain listing mark to having loose panicle and fragrance concurrently；Maturity period to have concurrently loose panicle and
The single plant of fragrance is that the objective trait that maintainer should have carries out evaluation and screening by three, and objective trait includes：The time of infertility 85
It, 85 centimetres of plant height, 25 centimetres of spike length, grain number per spike 145, spike number 14, grain length-width ratio 3.8,23 grams of mass of 1000 kernel, setting percentage
88% etc..292 plants of single plant sowing, obtains F4For seed, with F4- 12 indicate；
6）Strain sows F4- 12 seeds and CMS line P3, each strain plant 35 plants, P3 points of three phases of CMS line
Sowing.Full heading time selects the blade of 5 single plants to carry out fragrance identification, and to having concurrently to not detaching strain with loose panicle character
The strain of loose panicle and fragrance is that the objective trait that maintainer should have carries out observation identification by three, selects the slotting board mark of strain 88
Note each inserts board strain and selects 5 plants to carry out pairs of test crosses with P3.Maturity period selection in inserting card mark strain has objective trait
192 plants of listing marks of single plant, be listed strain single plant sowing.F4Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, is removed without perfume (or spice)
Taste single plant retains and has dulcet single-strain seed；Pairs of test cross marks sowing.Obtain F5For seed, with F5- 12 indicate, and in pairs
Test cross seed, with F1- 123 indicate；
7）Strain sows F5- 12 and F1- 123 seeds, each strain plant 35 plants, and full heading time is to loose panicle character
Strain is not detached, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is maintainer by three
The objective trait that should have selects 28 slotting card marks of strain；The sterile plant of pairs of test cross ties up to full heading time observation and blooms
Habit is concentrated when selection is spent, opens the strain progress pollen sterility degree microscopy that the clever time is long, pollen loose powder is good, stigma exposing ratio is high,
Retain single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；Listed strain F5- 12 and
BC1F1- 123 mark sowing in pairs.F5Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits single plant, is retained
Have dulcet single-strain seed, obtains F6For seed, with F6- 12 indicate, and 1 generation seed of backcrossing, with BC1F1- 123 indicate；
8）Strain sows F6- 12 and BC1F1- 123 seeds, each strain plant 35 plants.Full heading time is to loose panicle
Shape does not detach strain, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is kept by three systems
It is the objective trait that should have, selects 25 slotting card marks of strain；The sterile plant of pairing backcrossing ties up to full heading time observation and opens
Flower habit is concentrated when selection is spent, opens the strain progress pollen sterility degree mirror that the clever time is long, pollen loose powder is good, stigma exposing ratio is high
Inspection retains single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；Listed strain F6-12
And BC2F1- 123 mark sowing in pairs.F6Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits single plant, is protected
The dulcet single-strain seed of tool is stayed, F is obtained7For seed, with F7- 12 indicate, and 2 generation seeds of backcrossing, with BC2F1- 123 indicate；
9）Strain sows F7- 12 and BC2F1- 123 seeds, each strain plant 35 plants, and full heading time is to loose panicle
Shape does not detach strain, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is kept by three systems
It is the objective trait that should have, selects 26 slotting card marks of strain；The sterile plant of pairing backcrossing ties up to full heading time observation and opens
Flower habit is concentrated when selection is spent, opens the strain progress pollen sterility degree mirror that the clever time is long, pollen loose powder is good, stigma exposing ratio is high
Inspection retains single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；Listed strain F7-12
And BC2F1- 123 mark sowing in pairs.F7Brown rice fragrance identification is carried out after the shelling of -12 seeds, removes British plain spirits single plant, retains tool
Dulcet single-strain seed, obtains F8For seed, with F8- 12 indicate, and 3 generation seeds of backcrossing, with BC3F1- 123 indicate；
10）Continue step 9）Until obtaining F10- 12 and BC5F1- 123 seeds.Strain sows F10- 12 and BC5F1- 123 seeds,
And three be Poa linn P4 and three systems ripe restorer P5 late.Continue backcross transformation, obtains BC6F1- 123 seeds；Utilize P4 and P5
To BC5F1- 123 carry out test cross, with the breeding time feature and restorability of the sterile strain of identification, obtain test cross F1- 1 and F1- 2 seeds；
Sowing F10- 12 strains, obtain F11- 12 seeds；
11）Strain sows F11- 12 and BC6F1- 123 seeds and test cross F1- 1 and F1- 2 each 30.To 30 test cross F1-
1 and F1- 2 carry out Yield comparison experiment, are filtered out respectively with the excellent F of loose panicle, aromatic flavour, Comprehensive Traits1-1、F1- 2 groups
It closes, corresponding F11- 12 and BC6F1- 123 strains are the maintainer strain being selected in and sterile line strain, that is, have been bred as loose panicle type
Fragrant rice CMS line " dissipating perfume 1A "；
" dissipating perfume 1A " coordinate force is strong, the fringe portions pest and disease damage such as hybrid yield high, rice is of fine quality, nutrition is good, anti-false smut of combo.
" dissipating perfume 1A " " dissipates 1 excellent 527 " of perfume, yield increased kind II excellent bright 86 increases per acre with the extensive 527 combo scented rice Hybrid Rice Combination in another name for Sichuan Province
Add yield 4%, reduce 60 yuan of dispenser cost, scented rice paddy purchasing price increases by 20%, can be that peasant increases economic benefit 320 per acre
Member.
Embodiment 2：
1）Select the rice strain of loose panicle typesp（P1）, it is maintainer Lu perfume (or spice) 618B to have fragrance three（P2）, CMS line
The anti-A of gold（P3）, three be Poa linn Minghui 2155（P4）With three systems ripe restorer another name for Sichuan Province extensive 527 late（P5）For hybrid strain；
2）Make hybridization P1/P2, F must be hybridized1Seed, with F1- 12 indicate；
3）Plant F1- 12 25 plants of plant, the maturity period eliminates pseudostationary, remaining true hybrid mixes sowing, obtains F2For seed, with
F2- 12 indicate；
4）Plant F2- 12 3620 plants of plant.Heading stage carries out fragrance identification to the blade for the loose panicle single plant isolated, and is listed
Label；Maturity period, sowing, obtained F respectively to having the single plant of loose panicle and fragrance concurrently3For seed, with F3- 12 indicate；
5）Strain sows F3- 12 seeds, each strain plant 35 plants, the loose panicle strain that full heading time isolates each strain
Blade carry out fragrance identification, and single plant and strain listing mark to having loose panicle and fragrance concurrently；Maturity period to have concurrently loose panicle and
The single plant of fragrance is that the objective trait that maintainer should have carries out evaluation and screening by three, and objective trait includes：The time of infertility 75
It, 80 centimetres of plant height, 25 centimetres of spike length, grain number per spike 115, spike number 15, grain length-width ratio 3.5,21 grams of mass of 1000 kernel, setting percentage
85% etc..292 plants of single plant sowing, obtains F4For seed, with F4- 12 indicate；
6）Strain sows F4- 12 seeds and CMS line P3, each strain plant 35 plants, P3 points of three phases of CMS line
Sowing.Full heading time selects the blade of 5 single plants to carry out fragrance identification, and to having concurrently to not detaching strain with loose panicle character
The strain of loose panicle and fragrance is that the objective trait that maintainer should have carries out observation identification by three, selects the slotting board mark of strain 75
Note each inserts board strain and selects 5 plants to carry out pairs of test crosses with P3.Maturity period selection in inserting card mark strain has objective trait
175 plants of listing marks of single plant；Listed strain single plant sowing, F4Brown rice fragrance identification is carried out after the shelling of -12 seeds, removes British plain spirits list
Strain retains and has dulcet single-strain seed；Pairs of test cross marks sowing.Obtain F5For seed, with F5- 12 expressions and pairs of test cross
Seed, with F1- 123 indicate；
7）Strain sows F5- 12 and F1- 123 seeds, each strain plant 35 plants, and full heading time is to loose panicle character
Strain is not detached, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is maintainer by three
The objective trait that should have selects 25 slotting card marks of strain；The sterile plant of pairs of test cross ties up to full heading time observation and blooms
Habit is concentrated when selection is spent, opens the strain progress pollen sterility degree microscopy that the clever time is long, pollen loose powder is good, stigma exposing ratio is high,
Retain pollen sterility degree up to 100% single plant, then corresponding male parent backcross transformation, and inserts card mark；Listed strain F5- 12 with
F1- 123 mark sowing in pairs.F5Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits single plant, reservation has
The single-strain seed of fragrance, obtains F6For seed, with F6- 12 indicate, and 1 generation seed of backcrossing, with BC1F1- 123 indicate；
8）Strain sows F6- 12 and BC1F1- 123 seeds, each strain plant 35 plants, and full heading time is to loose panicle
Shape does not detach strain, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is kept by three systems
It is the objective trait that should have, selects 28 slotting card marks of strain；The sterile plant of pairing backcrossing ties up to full heading time observation and opens
Flower habit is concentrated when selection is spent, opens the strain progress pollen sterility degree mirror that the clever time is long, pollen loose powder is good, stigma exposing ratio is high
Inspection retains single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；Listed strain F6-12
With BC1 F1- 123 mark sowing in pairs.F6Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits single plant, is protected
The dulcet single-strain seed of tool is stayed, F is obtained7For seed, with F7- 12 indicate, and 2 generation seeds of backcrossing, with BC2F1- 123 indicate；
9）Strain sows F7- 12 and BC2F1- 123 seeds, each strain plant 35 plants, and full heading time is to loose panicle
Shape does not detach strain, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is kept by three systems
It is the objective trait that should have, selects 23 slotting card marks of strain；The sterile plant of pairing backcrossing ties up to full heading time observation and opens
Flower habit is concentrated when selection is spent, opens the strain progress pollen sterility degree mirror that the clever time is long, pollen loose powder is good, stigma exposing ratio is high
Inspection retains single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；Listed strain F7-12
With BC2F1- 123 mark sowing in pairs.F7Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits single plant, is protected
The dulcet single-strain seed of tool is stayed, F is obtained8For seed, with F8- 12 indicate, and 3 generation seeds of backcrossing, with BC3F1- 123 indicate；
10）Continue step 9) until obtaining F10- 12 and BC5F1Seed.Strain sows F10- 12 and BC5F1- 123 seeds, and
Three be Poa linn P4 and three systems ripe restorer P5 late.Continue backcross transformation, obtains BC6F1- 123 seeds；Utilize P4 and P5 couples
BC5F1- 123 carry out test cross, with the breeding time feature and restorability of the sterile strain of identification, obtain test cross F1- 1 and F1- 2 seeds；It receives
Kind F10- 12 strains, obtain F11- 12 seeds；
11）Strain sows F11- 12 and BC6F1- 123 seeds and test cross F1-1、F1- 2 each 36.To 36 test cross F1-1
And F1- 2 carry out Yield comparison experiment, are filtered out respectively with the excellent F of loose panicle, aromatic flavour, Comprehensive Traits1-1、F1- 2 groups
It closes, corresponding F11- 12 strains and BC6F1- 123 strains are the maintainer strain being selected in and sterile line strain, that is, have been bred as dissipating
Fringe type perfume (or spice) rice CMS line " dissipates perfume 2A "；
" dissipating perfume 2A " coordinate force is strong, the fringe portions pest and disease damage such as hybrid yield high, rice is of fine quality, nutrition is good, anti-false smut of combo.
" dissipating perfume 2A " " dissipates 2 excellent 527 " of perfume, yield increased kind II excellent bright 86 increases per acre with the extensive 527 combo scented rice Hybrid Rice Combination in another name for Sichuan Province
Add yield 7%, reduce 90 yuan of dispenser cost, scented rice paddy purchasing price increases by 22%, can be that peasant increases economic benefit 400 per acre
Member.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification should all belong to the covering scope of the present invention.
Claims (2)
1. a kind of selection of loose panicle type perfume (or spice) rice CMS line, it is characterised in that the selection include following methods with
Step：
1）Juvenile stage：It is hybrid strain P1 to select the rice strain of loose panicle type, and fragrant rice three is that maintainer is P2, CMS line
For P3, three be Poa linn be P4, and ripe restorer is P5 late for three systems；
2）Hybridize combo and screening technique：
a）Make hybridization P1/P2, F must be hybridized1Seed, with F1- 12 indicate；
b）Plant F1- 12 15-25 plants of plant, the maturity period eliminates pseudostationary, mixes and receives true hybrid, obtains F2For seed, with F2- 12 indicate；
c）Plant F2- 12 3500-4000 plants of plant；Heading stage carries out fragrance identification to the blade for the loose panicle single plant isolated, and is listed
Label；Maturity period, sowing, obtained F respectively to having the single plant of loose panicle and fragrance concurrently3For seed, with F3- 12 indicate；
d）Strain sows F3- 12 seeds, each strain plant 35-40 plants, the loose panicle strain that full heading time isolates each strain
Blade carries out fragrance identification, and single plant and strain listing mark to having loose panicle and fragrance concurrently；Maturity period is to having loose panicle and perfume (or spice) concurrently
The single plant of taste is that the objective trait that maintainer should have carries out evaluation and screening by three, and objective trait includes：Time of infertility 80-
125 days, 65-85 centimetres of plant height, 20-30 centimetres of spike length, grain number per spike 90-140, spike number 12-18, grain length-width ratio 2.5-
4.5,20-30 grams of mass of 1000 kernel, setting percentage 85%-95%；250-300 plants of single plant sowing, obtains F4For seed, with F4- 12 indicate；
e）Strain sows F4- 12 seeds and CMS line P3, each strain plant 30-35 plants, and P3 points of three phases of CMS line are broadcast
Kind；Full heading time selects the blade of 5 single plants to carry out fragrance identification, and scattered to having concurrently to not detaching strain with loose panicle character
The strain of fringe and fragrance is that the objective trait that maintainer should have carries out observation identification by three, selects 80-90 slotting board marks of strain
Note each inserts board strain and selects 5 plants to carry out pairs of test crosses with P3；Maturity period selection in inserting card mark strain has objective trait
150-200 plants of listing marks of single plant, be listed strain single plant sowing；F4Brown rice fragrance identification, removal are carried out after the shelling of -12 strain seeds
British plain spirits single plant retains and has dulcet single-strain seed；Pairs of test cross marks sowing；Obtain F5For seed, with F5- 12 indicate, and
Pairs of test cross 1 generation seed, with F1- 123 indicate；
f）Strain sows F5- 12 and F1- 123 seeds, each strain plant 30-35 plants, full heading time to loose panicle character not
Strain is detached, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is that maintainer is answered by three
The objective trait having selects 20-30 slotting card marks of strain；The sterile plant of pairs of test cross ties up to full heading time observation and blooms
Habit is concentrated when selection is spent, opens the strain progress pollen sterility degree microscopy that the clever time is long, pollen loose powder is good, stigma exposing ratio is high,
Retain single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；Listed strain F5- 12 and
F1- 123 mark sowing in pairs；F5Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits single plant, reservation has
The single-strain seed of fragrance, obtains F6For seed, with F6- 12 indicate, and 1 generation seed of backcrossing, with BC1F1- 123 indicate；
g）Strain sows F6- 12 and BC1F1- 123 seeds, each strain plant 30-35 plants, and full heading time is to loose panicle
Shape does not detach strain, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is kept by three systems
It is the objective trait that should have, selects 20-30 slotting card marks of strain；The sterile plant of pairing backcrossing ties up to full heading time observation
Flowering habit is concentrated when selection is spent, opens the strain progress pollen sterility degree that the clever time is long, pollen loose powder is good, stigma exposing ratio is high
Microscopy retains single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；Listed strain F6-
12 and BC1F1- 123 mark sowing in pairs；F6Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits single plant,
Retain and have dulcet single-strain seed, obtains F7For seed, with F7- 12 indicate, and 2 generation seeds of backcrossing, with BC2F1- 123 indicate；
h）Strain sows F7- 12 and BC2F1- 123 seeds, each strain plant 30-35 plants, and full heading time is to loose panicle
Shape does not detach strain, selects the blade of 5 single plants to carry out fragrance identification, and the strain to having loose panicle and fragrance concurrently, is kept by three systems
It is the objective trait that should have, selects 20-30 slotting card marks of strain；The sterile plant of pairing backcrossing ties up to full heading time observation
Flowering habit is concentrated when selection is spent, opens the strain progress pollen sterility degree that the clever time is long, pollen loose powder is good, stigma exposing ratio is high
Microscopy retains single plant of the pollen sterility degree up to 100%, then corresponding male parent backcross transformation, and listing mark；Listed strain F7-
12 and BC2F1- 123 mark sowing in pairs；F7Brown rice fragrance identification is carried out after the shelling of -12 strain seeds, removes British plain spirits single plant,
Retain and have dulcet single-strain seed, obtains F8For seed, with F8- 12 indicate, and 3 generation seeds of backcrossing, with BC3F1- 123 indicate；
i）Continue step h) until obtaining F10- 12 and BC5F1- 123 seeds；Strain sows F10- 12 and BC5F1- 123 seeds, Yi Jisan
It is Poa linn P4 and three systems ripe restorer P5 late, each strain plants 30-35 plants；Continue backcross transformation, obtains BC6F1-123
Seed；Using P4 and P5 to BC5F1- 123 carry out test cross, with the breeding time feature and restorability of the sterile strain of identification, must survey
Hand over F1- 1 and F1- 2 seeds；Sowing F10- 12 strains, obtain F11- 12 seeds；
j）Strain sows F11- 12 and BC6F1- 123 seeds and test cross F1-1、F1- 2 each 30；To 30 test cross F1- 1 and F1-2
Yield comparison experiment is carried out, is filtered out respectively with the excellent F of loose panicle, aromatic flavour, Comprehensive Traits1-1、F1- 2 combinations, it is right
The F answered11- 12 strains and BC6F1- 123 strains are the maintainer strain being selected in and sterile line strain, that is, have been bred as loose panicle type perfume (or spice) rice
CMS line.
2. a kind of selection of loose panicle type perfume (or spice) rice CMS line according to claim 1, it is characterised in that described to dissipate
Fringe type strain P1 isspr8Orsp, fragrance three is that maintainer P2 is Lu perfume (or spice) 618B, and CMS line P3 is the anti-A of gold, and three systems are precocious
Restorer P4 is Minghui 2155, and ripe restorer P5 is another name for Sichuan Province extensive 527 late for three systems.
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| CN112075336B (en) * | 2020-07-30 | 2023-06-16 | 常德市农林科学研究院 | Method for efficiently breeding rice aroma type three-line sterile line |
| CN114731945A (en) * | 2022-04-07 | 2022-07-12 | 四川农业大学 | Fertility improvement method for sterile line of three-line hybrid rice and sterile line of three-line hybrid rice |
| CN114957420A (en) * | 2022-04-29 | 2022-08-30 | 福建省农业科学院水稻研究所 | Rice false smut related panicle gene spr9, protein and application |
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