CN104839010A - Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line - Google Patents
Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line Download PDFInfo
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Abstract
The invention relates to a breeding method of a high-quality disease-resistant high-temperature-resistant rice two-line sterile line, and belongs to the technical field of crop genetic breeding. The method is involved in rice germplasm breeding with long grain polymerization, high head rice rate and excellent economical characters such as high seed production temperature resistance and disease resistance, and the rice two-line sterile line with long grains, high head rice rate, high seed production temperature resistance, good out-crossing feature, stable fertility, good disease resistance and excellent comprehensive characters are bred. Great assembling is carried out by utilizing the sterile line, and a novel two-line hybrid rice assembly which is high in quality, disease-resistant and beneficial for seed production is screened out hopefully.
Description
Technical field
The present invention is specifically related to the selection of a kind of paddy rice High quality and diseases resistance high temperature resistant two-line sterile line kind matter, belongs to Crop Genetic Breeding technical field.
Background technology
Paddy rice is one of China and even world's staple food crop, therefore improves rice yield and seems particularly important, and wherein, high yield and high quality is disease-resistant is the important breeding objective of hybrid rice.Problems existing increasingly significant in current rice heterosis utilization process, embody a concentrated expression of that per unit area yield increase rate is little, meter Zhi Yu resistance is without the series of problems such as breakthrough, hybrid rice seed production of hybrid seeds production risk be large, especially along with extreme weather is more and more frequent in recent years, greater risk is brought to Rice Production.Be rice production of hybrid seeds safety as Summer Low Temperature has a strong impact on two; Hot conditions constrains ripening rate and the rice quality of Rice Hybrid Seed Production and percentage of seedgermination and the production of hybrid rice large area; Bad weather conditions even induce breaking out of rice blast, result in the underproduction total crop failure etc. of the paddy rice breeding that part large area is produced, bring great production hidden danger to the grain security of China.Therefore, seeking and formulate excellent parental rice germ plasm resource, is the important technical of Genetic and breeding in rice improvement.This patent is intended starting with from two-line sterile line improvement, by being polymerized the excellent economical character such as long grain, high head rice rate and the production of hybrid seeds is high temperature resistant, blast resisting, be bred as the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, the two-line sterile line that disease resistance is good, Comprehensive Traits is excellent, and then by a large amount of combo, expect to obtain high yield, high-quality, disease-resistant, and be suitable for the two-line hybrid rice Combination nova that the multi-environment production of hybrid seeds produces.
Summary of the invention
The object of the present invention is to provide the selection of paddy rice high-quality, disease-resistant, high temperature resistant two-line sterile line kind matter, above-mentioned male sterile line is utilized to carry out the strong advantage hybrid rice new combination of a large amount of assembly, be expected to filter out high yield, high-quality, disease-resistant, and the two-line hybrid rice Combination nova that seed produces cost is low.
Method of the present invention comprises the following steps:
1) with long grain type height head rice rate kind-A, column cap is high temperature resistant, easy production of hybrid seeds kind-B and high resistant to rice blast kind-C for parent, make hybrid combination F
a/ F
b, F
a/ F
c, respectively with F
aB, F
aCrepresent; Plantation F
aB, F
aC, and hybridize F
aB/ F
aC, obtain a generation of reestablishing diplomatic relations, with F
aBCrepresent; Plantation F
aBCand gather in the crops F
' 2seed;
2) by F
' 2planting seed is planted in rice blast grave illness district, and employing is brought out kind and carried out bringing out morbidity, and high resistant to rice blast kind C is contrast, therefrom selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading ripening stage, carry out panicle blast qualification, therefrom select high resistant to rice blast and seed is long grain individual plant, results F
3seed;
3) F is sowed spring
3, in the summer high temperature heading maturing stage, select plant annesl good, the strain that ripening rate is high, Comprehensive Traits is excellent, by strain results seed, with long grain, high head rice rate kind A for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the F that grain type is long, head rice rate is high
4for strain;
4) repeat the authentication step of 2,3, therefrom select rice matter outward appearance good, grain type is long, head rice rate is high, the F that disease resistance is good
6strain;
5) with low-temperature-sensitive two-line sterile line for female parent, with selected F
6strain is paternal hybrid, obtains hybrid Fs
1generation; Plantation Fs
1in generation, backcross with corresponding male parent after heading, obtain BCs
1generation;
6) corresponding BCs is planted
1generation, respectively mixed receipts BC
1fs
1for seed; Plantation BC
1fs
1more than colony 2000 strain, therefrom select fertility good, seed elongated, stigma exposing ratio is high, and what Comprehensive Traits was excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, during short-day low temperature in autumn, and the selfed seed BC of the sterile strain of mixing results
1fs
2;
7) by BC
1fs
2direct-seeding planting is in rice blast grave illness district, and employing is brought out kind and carried out bringing out morbidity, and high resistant to rice blast kind C is contrast, therefrom selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading stage carries out panicle blast qualification, therefrom selects high resistant to rice blast and seed is long grain individual plant rice head, cuts root and stem of certain plants regeneration and moves to Hainan in Winter and expand numerous, results Fs
3seed; With long grain, high head rice rate kind A for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the strain that grain type is long, head rice rate is high;
8) repeat the authentication step of 7, until obtain grain long grain, head rice rate is high, outcrossing characteristic is good, stable fertility, the paddy rice two that disease resistance is good, Comprehensive Traits is excellent is sterile strain Fs
6.
9) adopt method that is potted plant, climatic cabinate simulation High Temperature Stress to process, during process, climate box humidity is 60%, and degree/day arranges as follows: 7:00-9:00,37 DEG C; 9:00-14:00,40 DEG C; 14:00-16:00,37 DEG C; 16:00-7:00 next day, 35 DEG C.With the corresponding CMS line of kind B for contrast, by above-mentioned selected sterile strain Fs
6in generation, transplanting was in basin alms bowl respectively by strain, and the strain of every basin 2, cuts off open grain husk flower before immigration, and individual plant bagging, often overlap 2 fringes; Under the prerequisite ensureing sufficient pollen amount, adopt same restorer to pollinate continuously 5 days, during taking-up, measure the spike length degree of blooming in high temperature treatment period from fringe top downwards, and record of listing, manage under whole potted plant being placed in natural conditions after process terminates.Investigate ripening rate after seed maturity, split clever rate and plumpness, select outcrossing seed-setting rate etc. to be better than the strain of the corresponding CMS line of kind B, the easy production of hybrid seeds of high temperature, cut root and stem of certain plants regeneration and move to the numerous acquisition Fs of Hainan in Winter expansion
7generation.
10) season morning low temperature, High aititude and climatic cabinate is adopted to be sterile strain Fs to selected two
7the sterility in generation and can numerous property identifying, and with low-temperature-sensitive two-line sterile line for contrast, select fertility stability and be better than contrast, the low low-temperature-sensitive two of critical temperature is sterile strain; Repeat above-mentioned fertility, head rice rate and resistance and production of hybrid seeds outcrossing rate authentication step, finally obtain the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, the two-line sterile line that disease resistance is good, Comprehensive Traits is excellent.
Described long grain type height head rice rate kind is that the rich sky of C10(is big, high-quality mid-season super hybrid rice praises excellent 99 seed selections and characteristic thereof, Agriculture of Anhui science, 2004,32 (3): 429), the column cap indica type maintainer II-32B of high temperature resistant, easy production of hybrid seeds rice variety or the paddy rich B(summer bright unit, Qi Huaxiong etc., Climatic regionalization is on the impact of the hybrid combination ripening rate that four male sterile lines are prepared. hubei agricultural science, 2004. 02.21-22); High resistant to rice blast kind is the seed selection of wide anti-13B or the wide anti-13A of paddy rich B(Indica Rice CMS line, Sanming City agricultural science and technology, 3rd phase in 2003), long grain type long-grained nonglutinous rice low-temperature-sensitive two-line sterile line Kingsoft s-2(Fujian section mirror No. 116th, word [2004]) be two be sterile gene donor.By hybridization, backcross; Nature and manually combine and wait authentication method, acquisition grain long grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, and outcrossing characteristic is good, stable fertility, the two-line sterile line that disease resistance is good, Comprehensive Traits is excellent.
outstanding advantages of the present invention
The long grain of grain that patent of the present invention is bred as, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, the two-line sterile line that disease resistance is good, Comprehensive Traits is excellent has following advantages: the long grain of (1) male sterile line seed, head rice rate is high, improves the Appearance Quality of Paddy Rice of the indica type first generation of hybrid.(2) male sterile line outcrossing characteristic is good, the production of hybrid seeds is high temperature resistant, it is low to split clever rate, overcomes the shortcoming that breeding of hybrid rice produces male sterile line non-refractory low humidity, improves hybrid seed yield and quality, reduce production cost.(3) two-line sterile line rice blast resistance is improved, overcome current most two-line hybrid rice combination high yield and high quality, but the deficiency of disease resistance difference, reduces the production hidden danger because of disease underproduction total crop failure in producing, and promotes high yield, high-quality, the high resistance two-line hybrid rice Combination nova large-area applications on producing.
Embodiment
embodiment 1:
1) represent with kind A with long grain type height head rice rate kind C10(), column cap is high temperature resistant, easy production of hybrid seeds kind II-32B (representing with kind B) and the wide anti-13B(of high resistant to rice blast kind represents with kind C) for parent, make hybrid combination F
a/ F
b, F
a/ F
c, respectively with F
aB, F
aCrepresent; Plantation F
aB, F
aC, and hybridize F
aB/ F
aC, obtain a generation of reestablishing diplomatic relations, with F
aBCrepresent; Plantation F
aBCand gather in the crops F
, 2seed;
2) by F
, 2seed is planted in rice blast grave illness district, Fujian Longyan Shanghai-Hangzhou tea ground, and employing is brought out No. 6, kind International Rice and carried out bringing out morbidity, and the wide anti-13B of high resistant to rice blast kind is contrast, therefrom selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading ripening stage, carry out panicle blast qualification, therefrom select high resistant to rice blast and seed is long grain individual plant, results F
3seed;
3) F is sowed spring
3, ripe in summer high temperature heading, select plant annesl good, the strain that ripening rate is high, Comprehensive Traits is excellent, by strain results seed, with long grain, high head rice rate kind C10 for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the F that grain type is long, head rice rate is high
4for strain;
4) repeat the authentication step of 2,3, therefrom select rice matter outward appearance good, grain type is long, head rice rate is high, the F that disease resistance is good
6strain;
5) with low-temperature-sensitive two-line sterile line Kingsoft s-2 for female parent, with selected F
6strain is paternal hybrid, obtains hybrid Fs
1generation; Plantation Fs
1in generation, backcross with corresponding male parent after heading, obtain BCs
1generation;
6) corresponding BCs is planted
1generation, respectively mixed receipts BC
1fs
1for seed; Plantation BC
1fs
1colony 2100 strain, therefrom select fertility good, seed elongated, stigma exposing ratio is high, and what Comprehensive Traits was excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, during short-day low temperature in autumn, the selfed seed BC of the sterile strain of mixing results
1fs
2;
7) by BC
1fs
2seed is planted in rice blast grave illness district, Fujian Longyan Shanghai-Hangzhou tea ground, and employing is brought out No. 6, kind International Rice and carried out bringing out morbidity, and the wide anti-13B of high resistant to rice blast kind is contrast, therefrom selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading stage carries out panicle blast qualification, therefrom selects high resistant to rice blast and seed is long grain individual plant rice head, cuts root and stem of certain plants regeneration and moves to Hainan in Winter and expand numerous, results Fs
3seed; With long grain, high head rice rate kind C10 for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the strain that grain type is long, head rice rate is high;
8) repeat the authentication step of 7, until obtain grain long grain, head rice rate is high, outcrossing characteristic is good, stable fertility, the paddy rice two that disease resistance is good, Comprehensive Traits is excellent is sterile strain Fs
6.
9) adopt method that is potted plant, climatic cabinate simulation High Temperature Stress to process, during process, climate box humidity is 60%, and degree/day arranges as follows: 7:00-9:00,37 DEG C; 9:00-14:00,40 DEG C; 14:00-16:00,37 DEG C; 16:00-7:00 next day, 35 DEG C.With CMS line II-32A for contrast, by above-mentioned selected sterile strain Fs
6in generation, transplanting was in basin alms bowl respectively by strain, and the strain of every basin 2, cuts off open grain husk flower before immigration, and individual plant bagging, often overlap 2 fringes; Under the prerequisite ensureing sufficient pollen amount, adopt restorer bright extensive 86 continuously pollination 5 days, during taking-up, measure the spike length degree of blooming in high temperature treatment period from fringe top downwards, and record of listing, manage under whole potted plant being placed in natural conditions after process terminates.Investigate ripening rate after seed maturity, split clever rate and plumpness, select outcrossing seed-setting rate etc. to be better than the strain of II-32A, the easy production of hybrid seeds of high temperature, cut root and stem of certain plants regeneration and move to the numerous acquisition Fs of Hainan in Winter expansion
7generation.
10) season morning low temperature, High aititude and climatic cabinate is adopted to be sterile strain Fs to selected two
7the sterility in generation and can numerous property identifying, and with low-temperature-sensitive two-line sterile line for contrast, select fertility stability and be better than contrast, the low low-temperature-sensitive two of critical temperature is sterile strain; Repeat above-mentioned fertility, head rice rate and resistance and production of hybrid seeds outcrossing rate authentication step, finally obtain the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, the two-line sterile line " Kingsoft s-3 " that disease resistance is good, Comprehensive Traits is excellent.
Advantage: two-line sterile line " Kingsoft s-3 " stable fertility (critical temperature≤23.5 DEG C) of the long grain of incubation, high head rice rate, high temperature resistant, the easy production of hybrid seeds, the indica type long grain of seed, bran point purple, grain weighs 26.2g, aspect ratio 2.75, and head rice rate reaches 63.1%; This stigma exposing ratio of sterile line is 71.6%, and wherein bilateral exposing ratio is 43.7%, and production of hybrid seeds reproductive output is high; And high resistant to rice blast, the first generation of hybrid output adopting this male sterile line to prepare is high, better resistance, and rice is of fine quality, and hybrid seed yield is high, and hybrid vigour is obvious.
embodiment 2:
1) represent with kind A with long grain type height head rice rate kind C10(), column cap is high temperature resistant, the easy production of hybrid seeds and the rich B of disease-resistant variety paddy (representing with kind B) and the wide anti-13B(of high resistant to rice blast kind represent with kind C) for parent, make hybrid combination F
a/ F
b, F
a/ F
c, respectively with F
aB, F
aCrepresent; Plantation F
aB, F
aC, and hybridize F
aB/ F
aC, obtain a generation of reestablishing diplomatic relations, with F
aBCrepresent; Plantation F
aBCand gather in the crops F
, 2seed;
2) by F
, 2seed is planted in rice blast grave illness district, Fujian Longyan Shanghai-Hangzhou tea ground, and employing is brought out No. 6, kind International Rice and carried out bringing out morbidity, and the wide anti-13B of high resistant to rice blast kind is contrast, therefrom selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading ripening stage, carry out panicle blast qualification, therefrom select high resistant to rice blast and seed is long grain individual plant, results F
3seed;
3) F is sowed spring
3, ripe in summer high temperature heading, select plant annesl good, the strain that ripening rate is high, Comprehensive Traits is excellent, by strain results seed, with long grain, high head rice rate kind C10 for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the F that grain type is long, head rice rate is high
4strain;
4) repeat the authentication step of 2,3, therefrom select rice matter outward appearance good, grain type is long, head rice rate is high, the F that disease resistance is good
6strain;
5) with low-temperature-sensitive two-line sterile line Kingsoft s-2 for female parent, with selected F
6strain is paternal hybrid, obtains hybrid Fs
1generation; Plantation hybrid Fs
1in generation, backcross with corresponding male parent after heading, obtain BCs
1generation;
6) corresponding BCs is planted
1generation, respectively mixed receipts BC
1fs
1for seed; Plantation BC
1fs
1colony 2000 strain, therefrom select fertility to be seed elongated, stigma exposing ratio is high, and what Comprehensive Traits was excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, during short-day low temperature in autumn, the selfed seed BC of the sterile strain of mixing results
1fs
2;
7) by BC
1fs
2seed is planted in rice blast grave illness district, Fujian Longyan Shanghai-Hangzhou tea ground, and employing is brought out No. 6, kind International Rice and carried out bringing out morbidity, and the wide anti-13B of high resistant to rice blast kind is contrast, therefrom selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading stage carries out panicle blast qualification, therefrom selects high resistant to rice blast and seed is long grain individual plant rice head, cuts root and stem of certain plants regeneration and moves to Hainan in Winter and expand numerous, results Fs
3seed; With long grain, high head rice rate kind C10 for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the strain that grain type is long, head rice rate is high;
8) repeat the authentication step of 7, until obtain grain long grain, head rice rate is high, outcrossing characteristic is good, stable fertility, the paddy rice two that disease resistance is good, Comprehensive Traits is excellent is sterile strain Fs
6.
9) adopt method that is potted plant, climatic cabinate simulation High Temperature Stress to process, during process, climate box humidity is 60%, and degree/day arranges as follows: 7:00-9:00,37 DEG C; 9:00-14:00,40 DEG C; 14:00-16:00,37 DEG C; 16:00-7:00 next day, 35 DEG C.With the rich A of CMS line paddy for contrast, by above-mentioned selected sterile strain Fs
6in generation, transplanting was in basin alms bowl respectively by strain, and the strain of every basin 2, cuts off open grain husk flower before immigration, and individual plant bagging, often overlap 2 fringes; Under the prerequisite ensureing sufficient pollen amount, adopt restorer Fujian extensive 3301 continuously pollination 5 days, during taking-up, measure the spike length degree of blooming in high temperature treatment period from fringe top downwards, and record of listing, manage under whole potted plant being placed in natural conditions after process terminates.Investigate ripening rate after seed maturity, split clever rate and plumpness, select outcrossing seed-setting rate etc. to be better than the strain of Gu Feng A, the easy production of hybrid seeds of high temperature, cut root and stem of certain plants regeneration and move to the numerous acquisition Fs of Hainan in Winter expansion
7generation.
10) season morning low temperature, High aititude and climatic cabinate is adopted to be sterile strain F to selected two
7the sterility in generation and can numerous property identifying, and with low-temperature-sensitive two-line sterile line for contrast, select fertility stability and be better than contrasting Kingsoft s-2, the low low-temperature-sensitive two of critical temperature is sterile strain; Repeat above-mentioned fertility, head rice rate and resistance and production of hybrid seeds outcrossing rate authentication step, finally obtain the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, the two-line sterile line " poly s " that disease resistance is good, Comprehensive Traits is excellent.
advantage:two-line sterile line " poly s " stable fertility (critical temperature≤23.5 DEG C) of the long grain be bred as, high head rice rate, high temperature resistant, the easy production of hybrid seeds, this male sterile line high resistant to rice blast, the long grain of seed is indica type, bran point purple, grain weighs 28.1g, aspect ratio 2.71, head rice rate 60.6%; Stigma exposing ratio is 68.2%, and wherein bilateral exposing ratio is 41.2%, and production of hybrid seeds reproductive output is high; The two-line hybrid rice output adopting this male sterile line to prepare is high, better resistance, and rice is of fine quality, and breeding cost is low, and production application potentiality are large.
embodiment 3:
1) represent with kind A with long grain type height head rice rate water kind C10(), column cap is high temperature resistant, easy production of hybrid seeds maintainer II-32B (representing with kind B) and the rich B(of high resistant to rice blast kind paddy represents with kind C) for parent, make hybrid combination F
a/ F
b, F
a/ F
c, respectively with F
aB, F
aCrepresent; Plantation F
aB, F
aC, and hybridize F
aB/ F
aC, obtain a generation of reestablishing diplomatic relations, with F
aBCrepresent; Plantation F
aBCand gather in the crops F
, 2seed;
2) by F
, 2seed is planted in rice blast grave illness district, Fujian Longyan Shanghai-Hangzhou tea ground, and employing is brought out kind " 946 " and carried out bringing out morbidity, and the rich B of high resistant to rice blast kind paddy is contrast, therefrom selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading ripening stage, carry out panicle blast qualification, therefrom select high resistant to rice blast and seed is long grain individual plant, results F
3seed;
3) F is sowed spring
3, ripe in summer high temperature heading, select plant annesl good, the strain that ripening rate is high, Comprehensive Traits is excellent, by strain results seed, with long grain, high head rice rate kind C10 for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the F that grain type is long, head rice rate is high
4strain;
4) repeat the authentication step of 2,3, therefrom select rice matter outward appearance good, grain type is long, head rice rate is high, the F that disease resistance is good
6strain;
5) with low-temperature-sensitive two-line sterile line Kingsoft s-2 for female parent, with selected F
6strain is paternal hybrid, obtains hybrid Fs
1generation; Plantation hybrid Fs
1in generation, backcross with corresponding male parent after heading, obtain BCs
1generation;
6) corresponding BCs is planted
1generation, respectively mixed receipts BC
1fs
1for seed; Plantation BC
1fs
1colony 2000 strain, therefrom select fertility to be seed elongated, stigma exposing ratio is high, and what Comprehensive Traits was excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, during short-day low temperature in autumn, the selfed seed BC of the sterile strain of mixing results
1fs
2;
7) by BC
1fs
2planting seed is planted in rice blast grave illness district, Fujian Longyan Shanghai-Hangzhou tea ground, and employing is brought out kind " 946 " and carried out bringing out morbidity, and the rich B of high resistant to rice blast kind paddy is contrast, selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading stage carries out panicle blast qualification, therefrom selects high resistant to rice blast and seed is long grain individual plant rice head, cuts root and stem of certain plants regeneration and moves to Hainan in Winter and expand numerous, results Fs
3seed; With long grain, high head rice rate kind C10 for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the strain that grain type is long, head rice rate is high;
8) repeat the authentication step of 7, until obtain grain long grain, head rice rate is high, outcrossing characteristic is good, stable fertility, the paddy rice two that disease resistance is good, Comprehensive Traits is excellent is sterile strain Fs
6.
9) adopt method that is potted plant, climatic cabinate simulation High Temperature Stress to process, during process, climate box humidity is 60%, and degree/day arranges as follows: 7:00-9:00,37 DEG C; 9:00-14:00,40 DEG C; 14:00-16:00,37 DEG C; 16:00-7:00 next day, 35 DEG C.With CMS line II-32A for contrast, by above-mentioned selected sterile strain Fs
6in generation, transplanting was in basin alms bowl respectively by strain, and the strain of every basin 2, cuts off open grain husk flower before immigration, and individual plant bagging, often overlap 2 fringes; Under the prerequisite ensureing sufficient pollen amount, adopt restorer bright extensive 63 continuously pollination 5 days, during taking-up, measure the spike length degree of blooming in high temperature treatment period from fringe top downwards, and record of listing, manage under whole potted plant being placed in natural conditions after process terminates.Investigate ripening rate after seed maturity, split clever rate and plumpness, select outcrossing seed-setting rate etc. to be better than the strain of II-32A, the easy production of hybrid seeds of high temperature, cut root and stem of certain plants regeneration and move to the numerous acquisition Fs of Hainan in Winter expansion
7generation.
10) season morning low temperature, High aititude and climatic cabinate is adopted to be sterile strain Fs to selected two
7the sterility in generation and can numerous property identifying, and with low-temperature-sensitive two-line sterile line for contrast, select fertility stability and be better than contrasting Kingsoft s-2, the low low-temperature-sensitive two of critical temperature is sterile strain; Repeat above-mentioned fertility, head rice rate and resistance and production of hybrid seeds outcrossing rate authentication step, finally obtain the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, the two-line sterile line " the poly-s-2 of gold " that disease resistance is good, Comprehensive Traits is excellent.
advantage:two-line sterile line " the poly-s-2 of the gold " stable fertility (critical temperature≤23.5 DEG C) of the long grain be bred as, high head rice rate, high temperature resistant, the easy production of hybrid seeds, this male sterile line rice quality is excellent, high resistant to rice blast, bran point purple, and grain weighs 26.3g, aspect ratio 2.81, head rice rate reaches 65.5%; Stigma exposing ratio is 73.8%, and wherein bilateral exposing ratio is 46.1%, and production of hybrid seeds reproductive output is high; The two-line hybrid rice output adopting this male sterile line to prepare is high, better resistance, and head rice rate is high, and mouthfeel is good, and hybrid seed yield is high, and cost is low, is expected to the two-line hybrid rice Combination nova being bred as large scale application.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. a selection for the high temperature resistant disease-resistant two-line sterile line kind matter of paddy rice high-quality, is characterized in that: comprise the following steps:
1) with long grain type, high head rice rate rice varieties-A, column cap is high temperature resistant, easy production of hybrid seeds kind-B and high resistant to rice blast kind-C for parent, make hybrid combination F
a/ F
b, F
a/ F
c, respectively with F
aB, F
aCrepresent; Plantation F
aB, F
aC, and hybridize F
aB/ F
aC, obtain a generation of reestablishing diplomatic relations, with F
aBCrepresent; Plantation F
aBCand gather in the crops F
, 2seed;
2) by F
, 2planting seed is planted in rice blast grave illness district, and employing is brought out kind and carried out bringing out morbidity, and high resistant to rice blast kind C is contrast, therefrom selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading-ripening stage, carry out panicle blast qualification, therefrom select high resistant to rice blast and seed is long grain individual plant, results F
3seed;
3) F is sowed spring
3, in the summer high temperature heading maturing stage, select plant annesl good, the strain that ripening rate is high, Comprehensive Traits is excellent, by strain results seed, with long grain, high head rice rate kind A for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the F that grain type is long, head rice rate is high
4strain;
4) repeat the authentication step of 2,3, therefrom select rice matter outward appearance good, grain type is long, head rice rate is high, the F that disease resistance is good
6strain;
5) with low-temperature-sensitive two-line sterile line for female parent, with selected F
6strain is paternal hybrid, obtains hybrid Fs
1generation; Plantation hybrid Fs
1in generation, backcross with corresponding male parent after heading, obtain BCs
1generation;
6) corresponding BCs is planted
1generation, respectively mixed receipts BC
1fs
1for seed; Plantation BC
1fs
1more than colony 2000 strain, therefrom select fertility good, seed elongated, stigma exposing ratio is high, and what Comprehensive Traits was excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, during short-day low temperature in autumn, and the selfed seed BC of the sterile strain of mixing results
1fs
2;
7) by BC
1fs
2seed is planted in rice blast grave illness district, and employing is brought out kind and carried out bringing out morbidity, and high resistant to rice blast kind C is contrast, therefrom selects leaf pest onset grade at the rice shoot of 0-1 level, transplants rice transplanting; Heading stage carries out panicle blast qualification, therefrom selects high resistant to rice blast and seed is long grain individual plant rice head, cuts root and stem of certain plants regeneration and moves to Hainan in Winter and expand numerous, results Fs
3seed; With long grain, high head rice rate kind A for contrast, adopt small rice polisher to process, therefrom select rice matter outward appearance good, the strain that grain type is long, head rice rate is high;
8) repeat the authentication step of 7, until obtain grain long grain, head rice rate is high, outcrossing characteristic is good, stable fertility, the paddy rice two that disease resistance is good, Comprehensive Traits is excellent is sterile strain Fs
6;
9) adopt method that is potted plant, climatic cabinate simulation High Temperature Stress to process, during process, climate box humidity is 60%, and degree/day arranges as follows: 7:00-9:00,37 DEG C; 9:00-14:00,40 DEG C; 14:00-16:00,37 DEG C; 16:00-7:00 next day, 35 DEG C, with the corresponding CMS line of kind B for contrast, by above-mentioned selected sterile strain F
6in generation, transplanting was in basin alms bowl respectively by strain, and the strain of every basin 2, cuts off open grain husk flower before immigration, and individual plant bagging, often overlap 2 fringes; Under the prerequisite ensureing sufficient pollen amount, same restorer is adopted to pollinate continuously 5 days, measure the spike length degree of blooming in high temperature treatment period downwards from fringe top during taking-up, and record of listing, manage under whole potted plant being placed in natural conditions after process terminates, investigate ripening rate after seed maturity, split clever rate and plumpness, select outcrossing seed-setting rate etc. to be better than the corresponding CMS line of kind B, the strain of the easy production of hybrid seeds of high temperature, cut root and stem of certain plants regeneration and move to the numerous acquisition Fs of Hainan in Winter expansion
7generation;
10) season morning low temperature, High aititude and climatic cabinate is adopted to be sterile strain Fs to selected two
7the sterility in generation and can numerous property identifying, and with low-temperature-sensitive two-line sterile line for contrast, select fertility stability and be better than contrast, the low low-temperature-sensitive two of critical temperature is sterile strain; Repeat above-mentioned fertility, head rice rate and resistance and production of hybrid seeds outcrossing rate authentication step, finally obtain the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, the two-line sterile line that disease resistance is good, Comprehensive Traits is excellent.
2. the selection of a kind of paddy rice High quality and diseases resistance high temperature resistant two-line sterile line kind matter according to claim 1, is characterized in that: described long grain type height head rice rate kind be new hybrid rice varieties praise excellent 99 male parent C10, column cap is high temperature resistant, the indica type maintainer II-32B of easy production of hybrid seeds rice variety or the rich B of paddy; High resistant to rice blast kind is wide anti-13B or the rich B of paddy, and long grain type rice variety is low-temperature-sensitive two-line sterile line is Kingsoft s-2.
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Address after: 354200 Tongyou Industrial Park, North Fujian Economic Development Zone, Jianyang City, Nanping City, Fujian Province, Phase 2, 2A-2 Patentee after: Fujian Jinshandu Seed Industry Development Co., Ltd. Address before: 354200 Tongyou Industrial Park, North Fujian Economic Development Zone, Jianyang City, Nanping City, Fujian Province, Phase 2, 2A-2 Patentee before: Kingsoft, Fujian all Development Co., Ltds |
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