CN105706913A - Breeding method of forage-use dry rice dual-purpose genic male sterility line - Google Patents
Breeding method of forage-use dry rice dual-purpose genic male sterility line Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a breeding method of a forage-use dry rice dual-purpose genic male sterility line, and belongs to the technical field of special rice breeding. The high-yield and high-crude-protein-content forage-use rice species is selected as the basic parent, the dual-purpose rice species with straw suitable for being used as fodder and the high-yield and strong-stress-resistance dry rice species are used as general parents, the dual-purpose maintaining line serves as a recurrent parent, the dry-cultivation, high-yield and high-nutrient dual-purpose mixed fodder rice dual-purpose genic male sterility line can be bred through scientific cross matching and targeted screening, the genic male sterility line is matched with a recovery line of the same type, and the high-yield and high-nutrient dual-purpose mixed fodder dual-purpose hybrid rice species can be bred. The method is scientific, efficient and easy to operate and has great significance in breeding the hybrid forage-use dry rice species.
Description
Technical field
The invention belongs to Special Rices breeding technical field, be specifically related to the selection of a kind of feeding upland rice two-line sterile line.
Background technology
That forage rice refers to is suitable at period of duration, on the basis of resistance, adopt Optimum Cultivation, can realize high yield (667 square metres yield more than 550 kilograms), high containing protein, high brown rice yield (being not less than 80%) and be suitable for feeding rice varieties." Forage Rice with High Protein Content " requires that brown rice gross protein value is more than or equal to 12%, the Breeding and popularization of " Forage Rice with High Protein Content ", not only can alleviate the Indica rice problem that difficulty is sold because of mouthfeel difference, also can realize southern area and substitute the imagination of field corn with forage rice brown rice.The live pig disease resistance raised with forage rice increases and meat taste is good, and feeding cost and Semen Maydis are raised and remained basically stable, and development potentiality is huge.And the aerial parts of Oryza sativa L. is all extracted, grain, straw, leaf mix as feedstuff, it is commonly called as mixed fodder rice, mixed fodder rice is compared with cutting green grass or young crops, Radix Glycyrrhizae, grain, all comparatively good rich in grain and fiber, nutritive value and physical characteristic, generally gather in dough stage or full ripe stage, gather in the crops also more convenient, be the preferred plan making feedstuff with rice.The all more general Caulis et Folium Oryzae of the gross protein value of Dual-purpose rice straw, protein digestibility, dry digestibility is high, " physics duricrust " that in glutathione transferasc grass, lignin and hemicellulose are combined closely by fat key is less, Relatively centralized, thus " nutrition closing " degree of Caulis et Folium Oryzae Middle nutrition material is less, thus pepsin, cellulase etc. are big with the contact surface of glutathione transferasc grass meal, animal can therefrom obtain more nutrient substance.Thus, utilize glutathione transferasc to cultivate mixed fodder rice can so that its Caulis et Folium Oryzae more meets feed request.
China's water resources ownership per capita 2400 tons, for the 1/4 of world's water resources ownership per capita, occupies the 109th, the world, and the economic loss caused due to hydropenia every year exceedes 200,000,000,000 yuan.And China's agricultural water accounts for the 80% of total water consumption at present, wherein Oryza sativa L. water consumption accounts for the 70% of agricultural water.It means that only the water consumption that produces of Oryza sativa L. just accounts for the 56% of whole nation total water consumption, therefore with the existing water resource of China, it is difficult to support the further development of tradition Oryza sativa L..Upland rice variety is without sufficient moisture, and water-saving amount, more than 50%, therefore can guarantee the another selection of grain security as country, utilizes nonirrigated farmland development High-yield Dry Land Rice, should become and maintain the newly selected of national food security.
Owing to the research work of forage rice is relatively slow, breeding research is carried out not, lacks theoretical direction in selection-breeding, adds difficulty for selection-breeding work again.The inventive method makees basis parent with high yield, brown rice gross protein value is more than 12%, brown rice yield is more than 80% forage rice kind, the upland rice variety being suitable for doing the glutathione transferasc kind of feedstuff and high yield strong stress resistance with Caulis et Folium Oryzae makees general parent, screened by the hybridization combo of science and objective trait, select can dry farming cultivation, high yield height nutrition the dual-purpose mixed fodder rice varieties of paddy stalk.The method scientific and efficient, the selection-breeding of forage rice kind is significant.
Summary of the invention
It is an object of the invention to provide a kind of can dry farming cultivation, combining go out the selection of the dual-purpose mixed fodder Hybrid Rice Varieties two-line sterile line of paddy stalk of high yield height nutrition.Gained sterile line and same kind of restorer group are joined, and can be bred as the dual-purpose mixed fodder double-line hybrid rice varieties of paddy stalk of high yield height nutrition.The selection scientific and efficient of the present invention easily operates, and the selection-breeding for fodder hybrid rice provides a kind of efficient new method, and the selection-breeding of forage rice kind is significant.
For achieving the above object, the present invention adopts the following technical scheme that
The selection of a kind of feeding upland rice two-line sterile line comprises the following steps:
(1)Juvenile stage: select high yield, brown rice gross protein value is more than 12%, brown rice yield is more than 80% forage rice kind makees basis parent (P), the upland rice variety (P2) of glutathione transferasc kind (P1) and high yield strong stress resistance makees general parent, and two is keep system (R) to make recurrent parent；
(2)Hybridization method for group matching: for making the forage rice kind (P) genetic constitution accounting in hybrid generation big, keep its comprehensive merit of high yield high protein, hybridize method for group matching below adopting:
First time hybridization: P × P1 → F1-1；P × P2 → F1-2；R × P → F1-3；
Second time hybridization: (P × F1-1) → BF1-1；(P × F1-2) → BF1-2；(F1-3× P) → BF1-3；
Third time hybridization: BF1-1×BF1-2→BF1-12；
4th hybridization: BF2-3×BF1-12→BF1-123；
5th hybridization: R × B2F5-123→BC1F1；
6th hybridization: R × BC1F4→BC2F1；
(3)Plantation and filial generation system of selection:
A. plant parent P, P1, P2, R respectively, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, R × P → F1-3；Plantation hybridization F respectively1For F1-1、F1-2、F1-3With parent P, florescence make hybridization P × F1-1、P×F1-2And F1-3× P, must backcross F1For BF1-1、BF1-2And BF1-3；
B. BF is planted respectively1-1、BF1-2And BF1-3, make hybridization BF florescence1-1×BF1-2→BF1-12, BF1-3Sowing obtains BF2-3；Plant BF respectively1-12And BF2-3, make hybridization BF florescence2-3×BF1-12→BF1-123；Plantation BF1-123Obtain BF2-123；
C. light seeding BF on nonirrigated farmland2-123Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain that tillering ability is strong, plants stems stalk and the vigorous rice seedling of leaf growth, select the individual plant 230-280 strain that rice straw is sturdy, blade keeps green not senilism after maturation, sowing obtains BF3-123；
D. BF is planted with strain mode dry farming3-123Each strain sows 90, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, each community retains that tillering ability is strong, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, period of maturation selects individual plant 200 strain that rice straw is sturdy, blade keeps green not senilism, the neat mud of individual plant cuts off sowing, and by Oryza glutinosa and rice straw listing mark respectively, rice straw carries out crude protein content mensuration, screen the seed that 30 parts of the highest rice straws of rice straw crude protein content are corresponding, obtain BF4-123；
E. strain sowing BF is continued4-123, make hybridization P × BF florescence4-123Obtain B2F1-123, dry farming plantation B2F1-123, obtain B2F2-123；Light seeding B on nonirrigated farmland2F2-123Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain that tillering ability is strong, plants stems stalk and the vigorous rice seedling of leaf growth, select individual plant 230 strain that rice straw is sturdy, blade keeps green not senilism after maturation, sowing obtains B2F3-123；
F. B is planted with strain mode dry farming2F3-123, each strain sows 90, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, period of maturation selects rice straw sturdy, blade keeps individual plant 200 strain of green not senilism, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 30 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain B2F4-123；
G. B is planted with strain mode dry farming2F4-123, each strain sows 50, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 20 strain of leaf growth, period of maturation selects rice straw sturdy, blade keeps green not senilism, tassel is big, individual plant 80 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 10 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain B2F5-123；
H. plant and plant 10 parts of B2F5-123Strain and R, make hybridization R × B florescence2F5-123Obtain BC1F1, dry farming plantation BC1F1, obtain BC1F2；Light seeding BC on nonirrigated farmland1F2Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removes the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain tillering ability by force, plants stems stalk and the vigorous rice seedling of leaf growth, select pollen sterility individual plant 80 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, and florescence observes pollen fertility, selects 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC1F3；
I. BC is planted with strain mode dry farming1F3, each strain sows 100, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, select pollen sterility individual plant 100 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, florescence observes pollen fertility, select pollen fertility good, individual plant 30 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 2 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain BC1F4；
J. respectively at outdoor cultivation R, growth cabinet plantation BC1F4, florescence, growth cabinet temperature was set to 23 DEG C, made hybridization R × BC1F4, obtain BC2F1；Dry farming plantation BC2F1, obtain BC2F2；Light seeding BC on nonirrigated farmland2F2Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removes the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain tillering ability by force, plants stems stalk and the vigorous rice seedling of leaf growth, select pollen sterility individual plant 80 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, and florescence observes pollen fertility, selects 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC2F3；
K. BC is planted with strain mode dry farming2F3, each strain sows 100, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, select pollen sterility individual plant 100 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, florescence observes pollen fertility, select pollen fertility good, individual plant 30 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 5 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain BC2F4；
L. 5 parts of BC of strain plantation2F4Carry out investigating screening to its economical character, pollen fertility, sterile conversion temperature, select the single-plant propagation seed that agronomy and the thorough and sterile conversion temperature of yield traits phase pollen sterility excellent, sterile and parent R are close, be namely bred as paddy stalk dual-purpose feedstuff upland rice two-line sterile line " lyS ".
Described high yield, the forage rice kind that brown rice gross protein value is more than 12%, brown rice yield is more than 80% are " golden morning 47 " or " Hunan is Xian 24 early "；Described glutathione transferasc kind is " southeast 202 "；The upland rice variety of described high yield strong stress resistance is " Shanghai drought 7 " or " facing drought 1 "；Described two is keep system for " Peiai 64S " or " good fortune eS1 ".
Beneficial effects of the present invention:
The present invention makees basis parent with high yield, brown rice gross protein value is more than 12%, brown rice yield is more than 80% forage rice kind, it is suitable for doing the glutathione transferasc kind of feedstuff with Caulis et Folium Oryzae, the upland rice variety of high yield strong stress resistance makees general parent, it is sterile make recurrent parent with excellent two, screened by the hybridization combo of science and objective trait, select the dual-purpose mixed fodder upland rice two-line sterile line of paddy stalk that can make dry farming cultivation, high yield height nutrition.The hybrid paddy rice that this forage rice sterile line group is allotted can do upland rice cultivation, and its yield is high, the crude protein content of Oryza glutinosa and Caulis et Folium Oryzae is above common rice varieties, dry land and mountain planting can be made full use of, save kind of a rice use water more than 60%, making full use of rice straw resource, every mu can increase income more than 400 yuan.This selection science, easy, efficient, selection-breeding and national food security to feeding upland rice variety are significant.
Detailed description of the invention
In order to make content of the present invention easily facilitate understanding, below in conjunction with detailed description of the invention, technical solutions according to the invention are described further, but the present invention is not limited only to this.
Embodiment 1:
(1)Juvenile stage: select high yield, brown rice gross protein value is more than 12%, forage rice kind that brown rice yield is more than 80% " gold early 47 " makees basis parent (P), glutathione transferasc kind " southeast 202 " (P1), the upland rice variety " Shanghai drought 7 " (P2) of high yield strong stress resistance makees general parent, and two is keep system " Peiai 64S " (R) to make recurrent parent；
(2)Plantation and filial generation select:
A. plant parent P, P1, P2, R respectively, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, R × P → F1-3；Plantation hybridization F respectively1For F1-1、F1-2、F1-3With parent P, florescence make hybridization P × F1-1、P×F1-2And F1-3× P, must backcross F1For BF1-1、BF1-2And BF1-3；
B. BF is planted respectively1-1、BF1-2And BF1-3, make hybridization BF florescence1-1×BF1-2→BF1-12, BF1-3Sowing obtains BF2-3；Plant BF respectively1-12And BF2-3, make hybridization BF florescence2-3×BF1-12→BF1-123；Plantation BF1-123Obtain BF2-123；
C. light seeding BF on nonirrigated farmland2-123Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain that tillering ability is strong, plants stems stalk and the vigorous rice seedling of leaf growth, select individual plant 230 strain that rice straw is sturdy, blade keeps green not senilism after maturation, sowing obtains BF3-123；
D. BF is planted with strain mode dry farming3-123Each strain sows 90, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, each community retains that tillering ability is strong, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, period of maturation selects individual plant 200 strain that rice straw is sturdy, blade keeps green not senilism, the neat mud of individual plant cuts off sowing, and by Oryza glutinosa and rice straw listing mark respectively, rice straw carries out crude protein content mensuration, screen the seed that 30 parts of the highest rice straws of rice straw crude protein content are corresponding, obtain BF4-123；
E. strain sowing BF is continued4-123, make hybridization P × BF florescence4-123Obtain B2F1-123, dry farming plantation B2F1-123, obtain B2F2-123；Light seeding B on nonirrigated farmland2F2-123Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain that tillering ability is strong, plants stems stalk and the vigorous rice seedling of leaf growth, select individual plant 230 strain that rice straw is sturdy, blade keeps green not senilism after maturation, sowing obtains B2F3-123；
F. B is planted with strain mode dry farming2F3-123, each strain sows 90, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, period of maturation selects rice straw sturdy, blade keeps individual plant 200 strain of green not senilism, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 30 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain B2F4-123；
G. B is planted with strain mode dry farming2F4-123, each strain sows 50, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 20 strain of leaf growth, period of maturation selects rice straw sturdy, blade keeps green not senilism, tassel is big, individual plant 80 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 10 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain B2F5-123；
H. plant and plant 10 parts of B2F5-123Strain and R, make hybridization R × B florescence2F5-123Obtain BC1F1, dry farming plantation BC1F1, obtain BC1F2；Light seeding BC on nonirrigated farmland1F2Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removes the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain tillering ability by force, plants stems stalk and the vigorous rice seedling of leaf growth, select pollen sterility individual plant 80 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, and florescence observes pollen fertility, selects 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC1F3；
I. BC is planted with strain mode dry farming1F3, each strain sows 100, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, select pollen sterility individual plant 100 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, florescence observes pollen fertility, select pollen fertility good, individual plant 30 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 2 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain BC1F4；
J. respectively at outdoor cultivation R, growth cabinet plantation BC1F4, florescence, growth cabinet temperature was set to 23 DEG C, made hybridization R × BC1F4, obtain BC2F1；Dry farming plantation BC2F1, obtain BC2F2；Light seeding BC on nonirrigated farmland2F2Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removes the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain tillering ability by force, plants stems stalk and the vigorous rice seedling of leaf growth, select pollen sterility individual plant 80 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, and florescence observes pollen fertility, selects 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC2F3；
K. BC is planted with strain mode dry farming2F3, each strain sows 100, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, select pollen sterility individual plant 100 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, florescence observes pollen fertility, select pollen fertility good, individual plant 30 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 5 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain BC2F4；
L. 5 parts of BC of strain plantation2F4Carry out investigating screening to its economical character, pollen fertility, sterile conversion temperature, select the single-plant propagation seed that agronomy and the thorough and sterile conversion temperature of yield traits phase pollen sterility excellent, sterile and parent R are close, be namely bred as paddy stalk dual-purpose feedstuff upland rice two-line sterile line " lyS-1 ".
" lyS-1 " can allot the dual-purpose mixed fodder hybridization upland rice variety of paddy stalk with same type restorer group, this hybridization upland rice variety can be made the crude protein content of upland rice cultivation and yield height, Oryza glutinosa and Caulis et Folium Oryzae and be above common rice varieties, dry land and mountain planting can be made full use of, save kind of a rice use water more than 60%, making full use of rice straw resource, every mu can increase income more than 400 yuan.
Embodiment 2:
(1)Juvenile stage: select high yield, brown rice gross protein value is more than 12%, forage rice kind that brown rice yield is more than 80% " Hunan is Xian 24 early " makees basis parent (P), glutathione transferasc kind " southeast 202 " (P1), the upland rice variety " facing drought 1 " (P2) of high yield strong stress resistance makees general parent, and two is keep system " good fortune eS1 " (R) to make recurrent parent；
(2)Plantation and filial generation select:
A. plant parent P, P1, P2, R respectively, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, R × P → F1-3；Plantation hybridization F respectively1For F1-1、F1-2、F1-3With parent P, florescence make hybridization P × F1-1、P×F1-2And F1-3× P, must backcross F1For BF1-1、BF1-2And BF1-3；
B. BF is planted respectively1-1、BF1-2And BF1-3, make hybridization BF florescence1-1×BF1-2→BF1-12, BF1-3Sowing obtains BF2-3；Plant BF respectively1-12And BF2-3, make hybridization BF florescence2-3×BF1-12→BF1-123；Plantation BF1-123Obtain BF2-123；
C. light seeding BF on nonirrigated farmland2-123Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain that tillering ability is strong, plants stems stalk and the vigorous rice seedling of leaf growth, select individual plant 280 strain that rice straw is sturdy, blade keeps green not senilism after maturation, sowing obtains BF3-123；
D. BF is planted with strain mode dry farming3-123Each strain sows 90, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, each community retains that tillering ability is strong, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, period of maturation selects individual plant 200 strain that rice straw is sturdy, blade keeps green not senilism, the neat mud of individual plant cuts off sowing, and by Oryza glutinosa and rice straw listing mark respectively, rice straw carries out crude protein content mensuration, screen the seed that 30 parts of the highest rice straws of rice straw crude protein content are corresponding, obtain BF4-123；
E. strain sowing BF is continued4-123, make hybridization P × BF florescence4-123Obtain B2F1-123, dry farming plantation B2F1-123, obtain B2F2-123；Light seeding B on nonirrigated farmland2F2-123Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain that tillering ability is strong, plants stems stalk and the vigorous rice seedling of leaf growth, select individual plant 230 strain that rice straw is sturdy, blade keeps green not senilism after maturation, sowing obtains B2F3-123；
F. B is planted with strain mode dry farming2F3-123, each strain sows 90, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, period of maturation selects rice straw sturdy, blade keeps individual plant 200 strain of green not senilism, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 30 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain B2F4-123；
G. B is planted with strain mode dry farming2F4-123, each strain sows 50, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 20 strain of leaf growth, period of maturation selects rice straw sturdy, blade keeps green not senilism, tassel is big, individual plant 80 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 10 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain B2F5-123；
H. plant and plant 10 parts of B2F5-123Strain and R, make hybridization R × B florescence2F5-123Obtain BC1F1, dry farming plantation BC1F1, obtain BC1F2；Light seeding BC on nonirrigated farmland1F2Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removes the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain tillering ability by force, plants stems stalk and the vigorous rice seedling of leaf growth, select pollen sterility individual plant 80 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, and florescence observes pollen fertility, selects 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC1F3；
I. BC is planted with strain mode dry farming1F3, each strain sows 100, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, select pollen sterility individual plant 100 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, florescence observes pollen fertility, select pollen fertility good, individual plant 30 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 2 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain BC1F4；
J. respectively at outdoor cultivation R, growth cabinet plantation BC1F4, florescence, growth cabinet temperature was set to 23 DEG C, made hybridization R × BC1F4, obtain BC2F1；Dry farming plantation BC2F1, obtain BC2F2；Light seeding BC on nonirrigated farmland2F2Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removes the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain tillering ability by force, plants stems stalk and the vigorous rice seedling of leaf growth, select pollen sterility individual plant 80 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, and florescence observes pollen fertility, selects 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC2F3；
K. BC is planted with strain mode dry farming2F3, each strain sows 100, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, select pollen sterility individual plant 100 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, florescence observes pollen fertility, select pollen fertility good, individual plant 30 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 5 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain BC2F4；
L. 5 parts of BC of strain plantation2F4Carry out investigating screening to its economical character, pollen fertility, sterile conversion temperature, select the single-plant propagation seed that agronomy and the thorough and sterile conversion temperature of yield traits phase pollen sterility excellent, sterile and parent R are close, be namely bred as paddy stalk dual-purpose feedstuff upland rice two-line sterile line " lyS-2 ".
" lyS-2 " can allot the dual-purpose mixed fodder hybridization upland rice variety of paddy stalk with same type restorer group, this hybridization upland rice variety can be made the crude protein content of upland rice cultivation and yield height, Oryza glutinosa and Caulis et Folium Oryzae and be above common rice varieties, dry land and mountain planting can be made full use of, save kind of a rice use water more than 60%, making full use of rice straw resource, every mu can increase income more than 400 yuan.
The foregoing is only presently preferred embodiments of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to the covering scope of the present invention.
Claims (2)
1. the selection of a feeding upland rice two-line sterile line, it is characterised in that: described selection comprises the following steps:
(1)Juvenile stage: select high yield, brown rice gross protein value is more than 12%, brown rice yield is more than 80% forage rice kind makees basis parent (P), the upland rice variety (P2) of glutathione transferasc kind (P1) and high yield strong stress resistance makees general parent, and two is keep system (R) to make recurrent parent；
(2)Plantation and filial generation system of selection:
A. plant parent P, P1, P2, R respectively, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, R × P → F1-3；Plantation hybridization F respectively1For F1-1、F1-2、F1-3With parent P, florescence make hybridization P × F1-1、P×F1-2And F1-3× P, must backcross F1For BF1-1、BF1-2And BF1-3；
B. BF is planted respectively1-1、BF1-2And BF1-3, make hybridization BF florescence1-1×BF1-2→BF1-12, BF1-3Sowing obtains BF2-3；Plant BF respectively1-12And BF2-3, make hybridization BF florescence2-3×BF1-12→BF1-123；Plantation BF1-123Obtain BF2-123；
C. light seeding BF on nonirrigated farmland2-123Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain that tillering ability is strong, plants stems stalk and the vigorous rice seedling of leaf growth, select the individual plant 230-280 strain that rice straw is sturdy, blade keeps green not senilism after maturation, sowing obtains BF3-123；
D. BF is planted with strain mode dry farming3-123Each strain sows 90, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, each community retains that tillering ability is strong, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, period of maturation selects individual plant 200 strain that rice straw is sturdy, blade keeps green not senilism, the neat mud of individual plant cuts off sowing, and by Oryza glutinosa and rice straw listing mark respectively, rice straw carries out crude protein content mensuration, screen the seed that 30 parts of the highest rice straws of rice straw crude protein content are corresponding, obtain BF4-123；
E. strain sowing BF is continued4-123, make hybridization P × BF florescence4-123Obtain B2F1-123, dry farming plantation B2F1-123, obtain B2F2-123；Light seeding B on nonirrigated farmland2F2-123Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, remove the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain that tillering ability is strong, plants stems stalk and the vigorous rice seedling of leaf growth, select individual plant 230 strain that rice straw is sturdy, blade keeps green not senilism after maturation, sowing obtains B2F3-123；
F. B is planted with strain mode dry farming2F3-123, each strain sows 90, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, period of maturation selects rice straw sturdy, blade keeps individual plant 200 strain of green not senilism, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 30 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain B2F4-123；
G. B is planted with strain mode dry farming2F4-123, each strain sows 50, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 20 strain of leaf growth, period of maturation selects rice straw sturdy, blade keeps green not senilism, tassel is big, individual plant 80 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 10 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain B2F5-123；
H. plant and plant 10 parts of B2F5-123Strain and R, make hybridization R × B florescence2F5-123Obtain BC1F1, dry farming plantation BC1F1, obtain BC1F2；Light seeding BC on nonirrigated farmland1F2Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removes the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain tillering ability by force, plants stems stalk and the vigorous rice seedling of leaf growth, select pollen sterility individual plant 80 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, and florescence observes pollen fertility, selects 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC1F3；
I. BC is planted with strain mode dry farming1F3, each strain sows 100, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, select pollen sterility individual plant 100 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, florescence observes pollen fertility, select pollen fertility good, individual plant 30 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 2 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain BC1F4；
J. respectively at outdoor cultivation R, growth cabinet plantation BC1F4, florescence, growth cabinet temperature was set to 23 DEG C, made hybridization R × BC1F4, obtain BC2F1；Dry farming plantation BC2F1, obtain BC2F2；Light seeding BC on nonirrigated farmland2F2Sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removes the Seedling that tillering ability is poor, plants stems stalk is thin and delicate, retain tillering ability by force, plants stems stalk and the vigorous rice seedling of leaf growth, select pollen sterility individual plant 80 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, and florescence observes pollen fertility, selects 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC2F3；
K. BC is planted with strain mode dry farming2F3, each strain sows 100, sow spray and water a water, whole seedling stage later does not water, after 28 days seedling stages, rice shoot is carried out screening of drought resistance, removal tillering ability is poor, the Seedling that plants stems stalk is thin and delicate, it is strong that each community retains tillering ability, plants stems stalk and vigorous rice seedling 40 strain of leaf growth, select pollen sterility individual plant 100 strain listing mark thoroughly florescence, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, florescence observes pollen fertility, select pollen fertility good, individual plant 30 strain that setting percentage is high, the neat mud of individual plant cuts off sowing, by Oryza glutinosa and rice straw listing mark respectively, Oryza glutinosa go out rough after and rice straw carry out crude protein content mensuration respectively, screening brown rice crude protein content is higher than 12%, and the strain 5 parts that the rice straw crude protein content of correspondence is higher than 7%, obtain BC2F4；
L. 5 parts of BC of strain plantation2F4Carry out investigating screening to its economical character, pollen fertility, sterile conversion temperature, select the single-plant propagation seed that agronomy and the thorough and sterile conversion temperature of yield traits phase pollen sterility excellent, sterile and parent R are close, be namely bred as paddy stalk dual-purpose feedstuff upland rice two-line sterile line " lyS ".
2. the selection of feeding upland rice two-line sterile line according to claim 1, it is characterised in that: described high yield, the forage rice kind that brown rice gross protein value is more than 12%, brown rice yield is more than 80% are " gold early 47 " or " Hunan is Xian 24 early "；Described glutathione transferasc kind is " southeast 202 "；The upland rice variety of described high yield strong stress resistance is " Shanghai drought 7 " or " facing drought 1 "；Described two is keep system for " Peiai 64S " or " good fortune eS1 ".
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1985576A (en) * | 2006-08-15 | 2007-06-27 | 陈立云 | Bilinear process of producing early rice hybrid seed |
| CN104137765A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of high-stress resistance elongated upper internode two-line genic male sterile line |
| CN104488702A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of dual-purpose sterile lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
| CN104839010A (en) * | 2015-05-21 | 2015-08-19 | 福建金山都发展有限公司 | Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1985576A (en) * | 2006-08-15 | 2007-06-27 | 陈立云 | Bilinear process of producing early rice hybrid seed |
| CN104137765A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of high-stress resistance elongated upper internode two-line genic male sterile line |
| CN104488702A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of dual-purpose sterile lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
| CN104839010A (en) * | 2015-05-21 | 2015-08-19 | 福建金山都发展有限公司 | Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line |
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