CN105706913B - A kind of selection of feeding upland rice two-line sterile line - Google Patents

A kind of selection of feeding upland rice two-line sterile line Download PDF

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CN105706913B
CN105706913B CN201610130879.0A CN201610130879A CN105706913B CN 105706913 B CN105706913 B CN 105706913B CN 201610130879 A CN201610130879 A CN 201610130879A CN 105706913 B CN105706913 B CN 105706913B
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rice
seedling
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plants
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CN105706913A (en
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章清杞
陈幼玉
王和寿
朱海彬
郑长林
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Fujian Agriculture and Forestry University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

Abstract

The invention discloses a kind of selection of feeding upland rice two-line sterile line, belong to Special Rices breeding technical field.The present invention makees basic parent from high yield, the feed rice varieties of high gross protein value, the upland rice variety for being adapted to the glutathione transferasc kind and high yield strong stress resistance for making feed with straw makees general parent, two be that maintainer makees recurrent parent, screened by the hybridization combo and objective trait of science, selecting can be with the dual-purpose mixed fodder rice two-line sterile line of the paddy stalk of dry farming cultivation, high yield high nutrition, the restorer of the sterile line and same type assembles, and can be bred as the dual-purpose mixed fodder double-line hybrid rice varieties of paddy stalk of high yield high nutrition.The inventive method is scientific and efficient, easily operated, and the seed selection to the feeding upland rice variety of hybridization is significant.

Description

A kind of selection of feeding upland rice two-line sterile line
Technical field
The invention belongs to Special Rices breeding technical field, and in particular to a kind of seed selection side of feeding upland rice two-line sterile line Method.
Background technology
Forage rice refers to breeding time is suitable, on the basis of resistance, using Optimum Cultivation, can realize high yield（667 squares More than 550 kilograms of rice yield）, it is high containing protein, high brown rice yield（Not less than 80%）And it is adapted to feeding rice varieties." high egg White forage rice " requires that brown rice gross protein value is more than or equal to 12%, the Breeding and popularization of " Forage Rice with High Protein Content ", can not only delay Indica rice is solved because of poor taste and difficulty the problem of selling, can also realize that southern area substitutes the imagination of field corn with forage rice brown rice. The live pig disease resistance raised with forage rice increases and meat taste is good, and feeding cost remains basically stable with corn raising, sends out Exhibition has a high potential.And all extract the aerial part of rice, grain, stalk, leaf mix as feed, and it is mixed to be commonly called as Close forage rice, mixed fodder rice is compared with cutting green grass or young crops, hay, grain, rich in grain and fiber, nutritive value and physical characteristic all compared with To be good, generally gathered in dough stage or full ripe stage, harvest is also more convenient, is the preferred plan for making feed with rice.Paddy stalk two It is high with all more general straw of the gross protein value of rice straw, protein digestibility, dry digestibility, in glutathione transferasc grass " physics duricrust " less, Relatively centralized that lignin and hemicellulose are combined closely by fat key, so as to nutrients in straw Degree is smaller, thus the contact surface of pepsin, cellulase etc. and glutathione transferasc grass meal is big, animal for " the nutrition closing " of matter More nutriment can therefrom be obtained.Thus, it can cause its straw more using glutathione transferasc to cultivate mixed fodder rice Meet feed request.
2400 tons of China's water resources ownership per capita, it is the 1/4 of world's water resources ownership per capita, occupies the 109th, the world, Every year because economic loss caused by water shortage exceedes 200,000,000,000 yuan.And China's agricultural water accounts for the 80% of total water consumption at present, its reclaimed water Rice water consumption accounts for the 70% of agricultural water.It means that the production water consumption of only rice just accounts for the 56% of national total water consumption, therefore With the existing water resource in China, it is difficult to support the further development of traditional rice.Upland rice variety is without sufficient moisture, water-saving amount More than 50%, therefore the another selection of grain security can be ensured as country, develop High-yield Dry Land Rice using nonirrigated farmland, should turned into and maintain The new selection of national food security.
Because the research work of forage rice is slower, breeding research is carried out not enough, and theoretical direction is lacked in seed selection, is again choosing Educate work and add difficulty.The inventive method is more than the feed that 12%, brown rice yield is more than 80% with high yield, brown rice gross protein value Rice varieties make basic parent, and the upland rice variety for being adapted to the glutathione transferasc kind and high yield strong stress resistance for making feed with straw makees one As parent, screened by the hybridization combo and objective trait of science, selecting can be with dry farming cultivation, the paddy stalk two of high yield high nutrition With mixed fodder rice varieties.This method is scientific and efficient, and the seed selection to feed rice varieties is significant.
The content of the invention
It is an object of the invention to provide it is a kind of can with dry farming cultivation, combining go out high yield high nutrition paddy stalk it is dual-purpose mixed Close the selection of feed Hybrid Rice Varieties two-line sterile line.The restorer of gained sterile line and same type assembles, and can be bred as The dual-purpose mixed fodder double-line hybrid rice varieties of paddy stalk of high yield high nutrition.The selection of the present invention is scientific and efficient easy to operate, is The seed selection of fodder hybrid rice provides a kind of efficient new method, and the seed selection to feed rice varieties is significant.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of selection of feeding upland rice two-line sterile line comprises the following steps：
（1）Juvenile stage：High yield, brown rice gross protein value is selected to be more than the feed rice varieties that 12%, brown rice yield is more than 80% Make basic parent（P）, glutathione transferasc kind（P1）With the upland rice variety of high yield strong stress resistance（P2）Make general parent, two systems are protected Hold and be（R）Make recurrent parent；
（2）Hybridize method for group matching：To make feed rice varieties（P）Genetic constitution accounting in hybrid generation is big, keeps it High yield high protein integrates merit, using following hybridization method for group matching：
Hybridize for the first time：P×P1→F1-1；P×P2→F1-2；R×P→F1-3；
Second of hybridization：（P×F1-1）→BF1-1；（P×F1-2）→BF1-2；（F1-3×P）→BF1-3；
Third time hybridizes：BF1-1×BF1-2→BF1-12；
4th hybridization：BF2-3×BF1-12→BF1-123；
5th hybridization：R×B2F5-123→BC1F1；
6th hybridization：R×BC1F4→BC2F1；
（3）Plantation and filial generation system of selection：
A. parent P, P1, P2, R are planted respectively, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, R × P → F1-3；Plantation hybridization F respectively1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1、P×F1-2And F1-3× P, is obtained It is returned F1For BF1-1、BF1-2And BF1-3；
B. BF is planted respectively1-1、BF1-2And BF1-3, florescence makees hybridization BF1-1×BF1-2→BF1-12, BF1-3Sowing obtains BF2-3；BF is planted respectively1-12And BF2-3, florescence makees hybridization BF2-3×BF1-12→BF1-123；Plant BF1-123Obtain BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sow spray and poured a water, do not watered in whole seedling stage afterwards, 28 days seedling stages Screening of drought resistance is carried out to rice shoot afterwards, removes tillering ability is poor, plant stalk is thin and delicate seedling, retain tillering ability by force, plant stalk and The vigorous rice seedling of leaf growth, the individual plant 230-280 strains that rice straw is sturdy, blade keeps green not early ageing, sowing are selected after ripe Obtain BF3-123；
D. BF is planted in dry farming in a manner of strain3-123, each strain sows 90, sow to spray and poured a water, afterwards Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade 200 plants of the individual plant of green not early ageing is kept, the neat mud of individual plant cuts off sowing, paddy and rice straw is distinguished into listing mark, rice straw is carried out Crude protein content determines, and seed corresponding to screening rice straw crude protein content 30 parts of rice straws of highest, obtains BF4-123；
E. strain sowing BF is continued4-123, florescence makees hybridization P × BF4-123Obtain B2F1-123, dry farming plantation B2F1-123, obtain B2F2-123；The light seeding B on nonirrigated farmland2F2-123, sowed spray and poured a water, do not watered in whole seedling stage afterwards, after 28 days seedling stages Screening of drought resistance is carried out to rice shoot, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and leaf The eugonic rice seedling of piece, 230 plants of the individual plant that rice straw is sturdy, blade keeps green not early ageing is selected after ripe, sowing obtains B2F3-123；
F. B is planted in dry farming in a manner of strain2F3-123, each strain sowing 90, sowed spray and poured a water, afterwards Whole seedling stage do not water, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes that tillering ability is poor, plant stalk is thin and delicate Seedling, each cell retain 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, maturity period selection rice straw is sturdy, Blade keeps 200 plants of the individual plant of green not early ageing, and the neat mud of individual plant cuts off sowing, and paddy and rice straw are distinguished into listing mark, paddy Go out rough rear and rice straw and carry out crude protein content measure respectively, screening brown rice crude protein content is higher than 12%, and the thick egg of corresponding rice straw Bai Hanliang is higher than 7% 30 parts of strain, obtains B2F4-123；
G. B is planted in dry farming in a manner of strain2F4-123, each strain sowing 50, sowed spray and poured a water, afterwards Whole seedling stage do not water, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes that tillering ability is poor, plant stalk is thin and delicate Seedling, each cell retain 20 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, maturity period selection rice straw is sturdy, Blade keeps green not early ageing, 80 plants of the individual plant that tassel is big, setting percentage is high, and the neat mud of individual plant cuts off sowing, by paddy and rice straw point Other listing mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, And corresponding rice straw crude protein content is higher than 7% 10 parts of strain, obtains B2F5-123；
H. kind plants 10 parts of B2F5-123Strain and R, florescence make hybridization R × B2F5-123Obtain BC1F1, dry farming plantation BC1F1, obtain BC1F2；The light seeding BC on nonirrigated farmland1F2, sowed spray and poured a water, do not watered in whole seedling stage afterwards, to seedling after 28 days seedling stages Seedling carries out screening of drought resistance, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and blade life Long vigorous rice seedling, florescence select pollen sterility thoroughly 80 plants of listing marks of individual plant, the artificial gas that shift-in temperature is 23 DEG C Wait case and cut pocket regeneration, florescence observation pollen fertility, select 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC1F3；
I. BC is planted in dry farming in a manner of strain1F3, each strain sows 100, sow to spray and poured a water, afterwards Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection pollen sterility is thorough 100 plants of listing marks of individual plant, shift-in temperature is that 23 DEG C of growth cabinet cuts pocket regeneration, florescence observation pollen fertility, choosing 30 plants of the individual plant that pollen fertility is good, setting percentage is high, the neat mud of individual plant cut off sowing, and paddy and rice straw are distinguished into listing mark, paddy Go out rough rear and rice straw and carry out crude protein content measure respectively, screening brown rice crude protein content is higher than 12%, and the thick egg of corresponding rice straw Bai Hanliang is higher than 7% 2 parts of strain, obtains BC1F4；
J. respectively at outdoor cultivation R, growth cabinet plantation BC1F4, florescence growth cabinet temperature setting is 23 DEG C, Make hybridization R × BC1F4, obtain BC2F1；BC is planted in dry farming2F1, obtain BC2F2；The light seeding BC on nonirrigated farmland2F2, sow spray and pour a water, Do not watered in whole seedling stage afterwards, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removal tillering ability is poor, plant stalk is thin Weak seedling, retain the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selects pollen sterility thoroughly individual plant 80 plants of listing marks, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation pollen fertility, select pollen fertility 10 sowings of individual plant good, that setting percentage is high obtain BC2F3；
K. BC is planted in dry farming in a manner of strain2F3, each strain sows 100, sow to spray and poured a water, afterwards Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection pollen sterility is thorough 100 plants of listing marks of individual plant, shift-in temperature is that 23 DEG C of growth cabinet cuts pocket regeneration, florescence observation pollen fertility, choosing 30 plants of the individual plant that pollen fertility is good, setting percentage is high, the neat mud of individual plant cut off sowing, and paddy and rice straw are distinguished into listing mark, paddy Go out rough rear and rice straw and carry out crude protein content measure respectively, screening brown rice crude protein content is higher than 12%, and the thick egg of corresponding rice straw Bai Hanliang is higher than 7% 5 parts of strain, obtains BC2F4；
L. strain plants 5 parts of BC2F4, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, selected Select agronomy and yield traits is excellent, sterile phase pollen sterility is thorough and sterile conversion temperature and parent R similar in single-plant propagation kind Son, that is, it is bred as the dual-purpose feed upland rice two-line sterile line " lyS " of paddy stalk.
The high yield, brown rice gross protein value are more than feed rice varieties of the 12%, brown rice yield more than 80% for " gold early 47 " Or " Hunan morning Xian 24 "；The glutathione transferasc kind is the " southeast 202 "；The upland rice variety of the high yield strong stress resistance is " Shanghai Drought 7 " or " facing drought 1 "；Described two be that maintainer is " Peiai 64S " or " good fortune eS1 ".
Beneficial effects of the present invention：
The present invention is more than feed rice varieties of the 12%, brown rice yield more than 80% with high yield, brown rice gross protein value and makees basis Parent, the glutathione transferasc kind for being adapted to make feed with straw, the upland rice variety of high yield strong stress resistance makees general parent, with excellent Two system's infertility make recurrent parent, are screened by the hybridization combo and objective trait of science, dry farming cultivation, high yield can be made by selecting The dual-purpose mixed fodder upland rice two-line sterile line of paddy stalk of high nutrition.The hybrid paddy rice that the forage rice sterile line assembles out can make upland rice cultivation Training, and its yield is high, the crude protein content of paddy and straw is above common rice varieties, can make full use of dry land and mountain region kind Plant, save kind of a rice water more than 60%, make full use of rice straw resource, every mu can increase income more than 400 yuan.The seed selection side Forensic science, easy, efficient, the seed selection and national food security to feeding upland rice variety are significant.
Embodiment
In order that content of the present invention easily facilitates understanding, with reference to embodiment to of the present invention Technical scheme is described further, but the present invention is not limited only to this.
Embodiment 1：
（1）Juvenile stage：High yield, brown rice gross protein value is selected to be more than the feed rice varieties that 12%, brown rice yield is more than 80% " gold early 47 " makees basic parent（P）, the glutathione transferasc kind " southeast 202 "（P1）, upland rice variety " the Shanghai drought of high yield strong stress resistance No. 7 "（P2）Make general parent, two be maintainer " Peiai 64S "（R）Make recurrent parent；
（2）Plantation and filial generation selection：
A. parent P, P1, P2, R are planted respectively, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, R × P → F1-3；Plantation hybridization F respectively1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1、P×F1-2And F1-3× P, is obtained It is returned F1For BF1-1、BF1-2And BF1-3；
B. BF is planted respectively1-1、BF1-2And BF1-3, florescence makees hybridization BF1-1×BF1-2→BF1-12, BF1-3Sowing obtains BF2-3；BF is planted respectively1-12And BF2-3, florescence makees hybridization BF2-3×BF1-12→BF1-123；Plant BF1-123Obtain BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sow spray and poured a water, do not watered in whole seedling stage afterwards, 28 days seedling stages Screening of drought resistance is carried out to rice shoot afterwards, removes tillering ability is poor, plant stalk is thin and delicate seedling, retain tillering ability by force, plant stalk and The vigorous rice seedling of leaf growth, 230 plants of the individual plant that rice straw is sturdy, blade keeps green not early ageing is selected after ripe, sowing obtains BF3-123；
D. BF is planted in dry farming in a manner of strain3-123, each strain sows 90, sow to spray and poured a water, afterwards Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade 200 plants of the individual plant of green not early ageing is kept, the neat mud of individual plant cuts off sowing, paddy and rice straw is distinguished into listing mark, rice straw is carried out Crude protein content determines, and seed corresponding to screening rice straw crude protein content 30 parts of rice straws of highest, obtains BF4-123；
E. strain sowing BF is continued4-123, florescence makees hybridization P × BF4-123Obtain B2F1-123, dry farming plantation B2F1-123, obtain B2F2-123；The light seeding B on nonirrigated farmland2F2-123, sowed spray and poured a water, do not watered in whole seedling stage afterwards, after 28 days seedling stages Screening of drought resistance is carried out to rice shoot, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and leaf The eugonic rice seedling of piece, 230 plants of the individual plant that rice straw is sturdy, blade keeps green not early ageing is selected after ripe, sowing obtains B2F3-123；
F. B is planted in dry farming in a manner of strain2F3-123, each strain sowing 90, sowed spray and poured a water, afterwards Whole seedling stage do not water, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes that tillering ability is poor, plant stalk is thin and delicate Seedling, each cell retain 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, maturity period selection rice straw is sturdy, Blade keeps 200 plants of the individual plant of green not early ageing, and the neat mud of individual plant cuts off sowing, and paddy and rice straw are distinguished into listing mark, paddy Go out rough rear and rice straw and carry out crude protein content measure respectively, screening brown rice crude protein content is higher than 12%, and the thick egg of corresponding rice straw Bai Hanliang is higher than 7% 30 parts of strain, obtains B2F4-123；
G. B is planted in dry farming in a manner of strain2F4-123, each strain sowing 50, sowed spray and poured a water, afterwards Whole seedling stage do not water, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes that tillering ability is poor, plant stalk is thin and delicate Seedling, each cell retain 20 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, maturity period selection rice straw is sturdy, Blade keeps green not early ageing, 80 plants of the individual plant that tassel is big, setting percentage is high, and the neat mud of individual plant cuts off sowing, by paddy and rice straw point Other listing mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, And corresponding rice straw crude protein content is higher than 7% 10 parts of strain, obtains B2F5-123；
H. kind plants 10 parts of B2F5-123Strain and R, florescence make hybridization R × B2F5-123Obtain BC1F1, dry farming plantation BC1F1, obtain BC1F2；The light seeding BC on nonirrigated farmland1F2, sowed spray and poured a water, do not watered in whole seedling stage afterwards, to seedling after 28 days seedling stages Seedling carries out screening of drought resistance, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and blade life Long vigorous rice seedling, florescence select pollen sterility thoroughly 80 plants of listing marks of individual plant, the artificial gas that shift-in temperature is 23 DEG C Wait case and cut pocket regeneration, florescence observation pollen fertility, select 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC1F3；
I. BC is planted in dry farming in a manner of strain1F3, each strain sows 100, sow to spray and poured a water, afterwards Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection pollen sterility is thorough 100 plants of listing marks of individual plant, shift-in temperature is that 23 DEG C of growth cabinet cuts pocket regeneration, florescence observation pollen fertility, choosing 30 plants of the individual plant that pollen fertility is good, setting percentage is high, the neat mud of individual plant cut off sowing, and paddy and rice straw are distinguished into listing mark, paddy Go out rough rear and rice straw and carry out crude protein content measure respectively, screening brown rice crude protein content is higher than 12%, and the thick egg of corresponding rice straw Bai Hanliang is higher than 7% 2 parts of strain, obtains BC1F4；
J. respectively at outdoor cultivation R, growth cabinet plantation BC1F4, florescence growth cabinet temperature setting is 23 DEG C, Make hybridization R × BC1F4, obtain BC2F1；BC is planted in dry farming2F1, obtain BC2F2；The light seeding BC on nonirrigated farmland2F2, sow spray and pour a water, Do not watered in whole seedling stage afterwards, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removal tillering ability is poor, plant stalk is thin Weak seedling, retain the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selects pollen sterility thoroughly individual plant 80 plants of listing marks, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation pollen fertility, select pollen fertility 10 sowings of individual plant good, that setting percentage is high obtain BC2F3；
K. BC is planted in dry farming in a manner of strain2F3, each strain sows 100, sow to spray and poured a water, afterwards Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection pollen sterility is thorough 100 plants of listing marks of individual plant, shift-in temperature is that 23 DEG C of growth cabinet cuts pocket regeneration, florescence observation pollen fertility, choosing 30 plants of the individual plant that pollen fertility is good, setting percentage is high, the neat mud of individual plant cut off sowing, and paddy and rice straw are distinguished into listing mark, paddy Go out rough rear and rice straw and carry out crude protein content measure respectively, screening brown rice crude protein content is higher than 12%, and the thick egg of corresponding rice straw Bai Hanliang is higher than 7% 5 parts of strain, obtains BC2F4；
L. strain plants 5 parts of BC2F4, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, selected Select agronomy and yield traits is excellent, sterile phase pollen sterility is thorough and sterile conversion temperature and parent R similar in single-plant propagation kind Son, that is, it is bred as the dual-purpose feed upland rice two-line sterile line " lyS-1 " of paddy stalk.
" lyS-1 " can assemble out the dual-purpose mixed fodder hybridization upland rice variety of paddy stalk, the hybridization upland rice with same type restorer Kind can make upland rice cultivation and yield height, the crude protein content of paddy and straw is above common rice varieties, can make full use of drought Field and mountain planting, save kind of a rice water more than 60%, make full use of rice straw resource, every mu can increase by 400 yuan of income with On.
Embodiment 2：
（1）Juvenile stage：High yield, brown rice gross protein value is selected to be more than the feed rice varieties that 12%, brown rice yield is more than 80% " Hunan morning Xian 24 " makees basic parent（P）, the glutathione transferasc kind " southeast 202 "（P1）, the upland rice variety of high yield strong stress resistance " facing drought 1 "（P2）Make general parent, two be maintainer " good fortune eS1 "（R）Make recurrent parent；
（2）Plantation and filial generation selection：
A. parent P, P1, P2, R are planted respectively, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, R × P → F1-3；Plantation hybridization F respectively1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1、P×F1-2And F1-3× P, is obtained It is returned F1For BF1-1、BF1-2And BF1-3；
B. BF is planted respectively1-1、BF1-2And BF1-3, florescence makees hybridization BF1-1×BF1-2→BF1-12, BF1-3Sowing obtains BF2-3；BF is planted respectively1-12And BF2-3, florescence makees hybridization BF2-3×BF1-12→BF1-123；Plant BF1-123Obtain BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sow spray and poured a water, do not watered in whole seedling stage afterwards, 28 days seedling stages Screening of drought resistance is carried out to rice shoot afterwards, removes tillering ability is poor, plant stalk is thin and delicate seedling, retain tillering ability by force, plant stalk and The vigorous rice seedling of leaf growth, 280 plants of the individual plant that rice straw is sturdy, blade keeps green not early ageing is selected after ripe, sowing obtains BF3-123；
D. BF is planted in dry farming in a manner of strain3-123, each strain sows 90, sow to spray and poured a water, afterwards Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade 200 plants of the individual plant of green not early ageing is kept, the neat mud of individual plant cuts off sowing, paddy and rice straw is distinguished into listing mark, rice straw is carried out Crude protein content determines, and seed corresponding to screening rice straw crude protein content 30 parts of rice straws of highest, obtains BF4-123；
E. strain sowing BF is continued4-123, florescence makees hybridization P × BF4-123Obtain B2F1-123, dry farming plantation B2F1-123, obtain B2F2-123；The light seeding B on nonirrigated farmland2F2-123, sowed spray and poured a water, do not watered in whole seedling stage afterwards, after 28 days seedling stages Screening of drought resistance is carried out to rice shoot, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and leaf The eugonic rice seedling of piece, 230 plants of the individual plant that rice straw is sturdy, blade keeps green not early ageing is selected after ripe, sowing obtains B2F3-123；
F. B is planted in dry farming in a manner of strain2F3-123, each strain sowing 90, sowed spray and poured a water, afterwards Whole seedling stage do not water, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes that tillering ability is poor, plant stalk is thin and delicate Seedling, each cell retain 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, maturity period selection rice straw is sturdy, Blade keeps 200 plants of the individual plant of green not early ageing, and the neat mud of individual plant cuts off sowing, and paddy and rice straw are distinguished into listing mark, paddy Go out rough rear and rice straw and carry out crude protein content measure respectively, screening brown rice crude protein content is higher than 12%, and the thick egg of corresponding rice straw Bai Hanliang is higher than 7% 30 parts of strain, obtains B2F4-123；
G. B is planted in dry farming in a manner of strain2F4-123, each strain sowing 50, sowed spray and poured a water, afterwards Whole seedling stage do not water, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes that tillering ability is poor, plant stalk is thin and delicate Seedling, each cell retain 20 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, maturity period selection rice straw is sturdy, Blade keeps green not early ageing, 80 plants of the individual plant that tassel is big, setting percentage is high, and the neat mud of individual plant cuts off sowing, by paddy and rice straw point Other listing mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, And corresponding rice straw crude protein content is higher than 7% 10 parts of strain, obtains B2F5-123；
H. kind plants 10 parts of B2F5-123Strain and R, florescence make hybridization R × B2F5-123Obtain BC1F1, dry farming plantation BC1F1, obtain BC1F2；The light seeding BC on nonirrigated farmland1F2, sowed spray and poured a water, do not watered in whole seedling stage afterwards, to seedling after 28 days seedling stages Seedling carries out screening of drought resistance, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and blade life Long vigorous rice seedling, florescence select pollen sterility thoroughly 80 plants of listing marks of individual plant, the artificial gas that shift-in temperature is 23 DEG C Wait case and cut pocket regeneration, florescence observation pollen fertility, select 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC1F3；
I. BC is planted in dry farming in a manner of strain1F3, each strain sows 100, sow to spray and poured a water, afterwards Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection pollen sterility is thorough 100 plants of listing marks of individual plant, shift-in temperature is that 23 DEG C of growth cabinet cuts pocket regeneration, florescence observation pollen fertility, choosing 30 plants of the individual plant that pollen fertility is good, setting percentage is high, the neat mud of individual plant cut off sowing, and paddy and rice straw are distinguished into listing mark, paddy Go out rough rear and rice straw and carry out crude protein content measure respectively, screening brown rice crude protein content is higher than 12%, and the thick egg of corresponding rice straw Bai Hanliang is higher than 7% 2 parts of strain, obtains BC1F4；
J. respectively at outdoor cultivation R, growth cabinet plantation BC1F4, florescence growth cabinet temperature setting is 23 DEG C, Make hybridization R × BC1F4, obtain BC2F1；BC is planted in dry farming2F1, obtain BC2F2；The light seeding BC on nonirrigated farmland2F2, sow spray and pour a water, Do not watered in whole seedling stage afterwards, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removal tillering ability is poor, plant stalk is thin Weak seedling, retain the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selects pollen sterility thoroughly individual plant 80 plants of listing marks, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation pollen fertility, select pollen fertility 10 sowings of individual plant good, that setting percentage is high obtain BC2F3；
K. BC is planted in dry farming in a manner of strain2F3, each strain sows 100, sow to spray and poured a water, afterwards Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection pollen sterility is thorough 100 plants of listing marks of individual plant, shift-in temperature is that 23 DEG C of growth cabinet cuts pocket regeneration, florescence observation pollen fertility, choosing 30 plants of the individual plant that pollen fertility is good, setting percentage is high, the neat mud of individual plant cut off sowing, and paddy and rice straw are distinguished into listing mark, paddy Go out rough rear and rice straw and carry out crude protein content measure respectively, screening brown rice crude protein content is higher than 12%, and the thick egg of corresponding rice straw Bai Hanliang is higher than 7% 5 parts of strain, obtains BC2F4；
L. strain plants 5 parts of BC2F4, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, selected Select agronomy and yield traits is excellent, sterile phase pollen sterility is thorough and sterile conversion temperature and parent R similar in single-plant propagation kind Son, that is, it is bred as the dual-purpose feed upland rice two-line sterile line " lyS-2 " of paddy stalk.
" lyS-2 " can assemble out the dual-purpose mixed fodder hybridization upland rice variety of paddy stalk, the hybridization upland rice with same type restorer Kind can make upland rice cultivation and yield height, the crude protein content of paddy and straw is above common rice varieties, can make full use of drought Field and mountain planting, save kind of a rice water more than 60%, make full use of rice straw resource, every mu can increase by 400 yuan of income with On.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification, it should all belong to the covering scope of the present invention.

Claims (1)

  1. A kind of 1. selection of feeding upland rice two-line sterile line, it is characterised in that：The selection comprises the following steps：
    （1）Juvenile stage：Select high yield, brown rice gross protein value to be more than feed rice varieties of the 12%, brown rice yield more than 80% and make base The upland rice variety P2 of plinth parent P, glutathione transferasc kind P1 and high yield strong stress resistance makees general parent, and two be that maintainer R takes turns Return parent；
    （2）Plantation and filial generation system of selection：
    A. parent P, P1, P2, R are planted respectively, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, R × P → F1-3；Point F Zhong Zhi not hybridized1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1、P×F1-2And F1-3× P, F must be returned1 For BF1-1、BF1-2And BF1-3；
    B. BF is planted respectively1-1、BF1-2And BF1-3, florescence makees hybridization BF1-1×BF1-2→BF1-12, BF1-3Sowing obtains BF2-3；Point BF is not planted1-12And BF2-3, florescence makees hybridization BF2-3×BF1-12→BF1-123；Plant BF1-123Obtain BF2-123；
    C. the light seeding BF on nonirrigated farmland2-123, sow spray and poured a water, do not watered in whole seedling stage afterwards, it is right after 28 days seedling stages Rice shoot carries out screening of drought resistance, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and blade Eugonic rice seedling, selects the individual plant 230-280 strains that rice straw is sturdy, blade keeps green not early ageing after ripe, sowing obtains BF3-123；
    D. BF is planted in dry farming in a manner of strain3-123, each strain sows 90, sow to spray and poured a water, whole afterwards Seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, each Cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade is kept 200 plants of the individual plant of green not early ageing, the neat mud of individual plant cut off sowing, paddy and rice straw are distinguished into listing mark, rice straw carries out thick egg White assay, seed corresponding to screening rice straw crude protein content 30 parts of rice straws of highest, obtains BF4-123；
    E. strain sowing BF is continued4-123, florescence makees hybridization P × BF4-123Obtain B2F1-123, dry farming plantation B2F1-123, obtain B2F2-123；The light seeding B on nonirrigated farmland2F2-123, sowed spray and poured a water, do not watered in whole seedling stage afterwards, after 28 days seedling stages Screening of drought resistance is carried out to rice shoot, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and leaf The eugonic rice seedling of piece, 230 plants of the individual plant that rice straw is sturdy, blade keeps green not early ageing is selected after ripe, sowing obtains B2F3-123；
    F. B is planted in dry farming in a manner of strain2F3-123, each strain sows 90, sow to spray and poured a water, whole afterwards Individual seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, often Individual cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade is protected Hold 200 plants of the individual plant of green not early ageing, the neat mud of individual plant cuts off sowing, and paddy and rice straw distinguished into listing mark, paddy go out it is rough after Crude protein content measure is carried out respectively with rice straw, and screening brown rice crude protein content is higher than 12%, and corresponding rice straw crude protein content 30 parts of strain higher than 7%, obtains B2F4-123；
    G. B is planted in dry farming in a manner of strain2F4-123, each strain sows 50, sow to spray and poured a water, whole afterwards Individual seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, often Individual cell retains 20 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade is protected Green not early ageing, 80 plants of the individual plant that tassel is big, setting percentage is high are held, the neat mud of individual plant cuts off sowing, paddy and rice straw are listed respectively Mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly Rice straw crude protein content be higher than 7% 10 parts of strain, obtain B2F5-123；
    H. kind plants 10 parts of B2F5-123Strain and R, florescence make hybridization R × B2F5-123Obtain BC1F1, dry farming plantation BC1F1, obtain BC1F2；The light seeding BC on nonirrigated farmland1F2, sowed spray and poured a water, do not watered in whole seedling stage afterwards, to seedling after 28 days seedling stages Seedling carries out screening of drought resistance, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and blade life Long vigorous rice seedling, florescence select pollen sterility thoroughly 80 plants of listing marks of individual plant, the artificial gas that shift-in temperature is 23 DEG C Wait case and cut pocket regeneration, florescence observation pollen fertility, select 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BC1F3；
    I. BC is planted in dry farming in a manner of strain1F3, each strain sows 100, sow to spray and poured a water, whole afterwards Seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, each Cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection pollen sterility is thoroughly single 100 plants of listing marks of strain, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation pollen fertility, select pollen 30 plants of the individual plant that fertility is good, setting percentage is high, the neat mud of individual plant cut off sowing, paddy and rice straw are distinguished into listing mark, paddy goes out rough Carry out crude protein content measure respectively with rice straw afterwards, screening brown rice crude protein content is higher than 12%, and corresponding rice straw crude protein contains Strain 2 part of the amount higher than 7%, obtains BC1F4；
    J. respectively at outdoor cultivation R, growth cabinet plantation BC1F4, florescence growth cabinet temperature setting is 23 DEG C, is made miscellaneous Hand over R × BC1F4, obtain BC2F1；BC is planted in dry farming2F1, obtain BC2F2；The light seeding BC on nonirrigated farmland2F2, sowed spray and poured a water, at it Whole seedling stage afterwards is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, and removal tillering ability is poor, plant stalk is thin and delicate Seedling, retain the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selects pollen sterility thoroughly 80 plants of individual plant Listing mark, shift-in temperature are that 23 DEG C of growth cabinet cuts pocket regeneration, florescence observation pollen fertility, select pollen fertility it is good, High 10 sowings of individual plant of setting percentage obtain BC2F3；
    K. BC is planted in dry farming in a manner of strain2F3, each strain sows 100, sow to spray and poured a water, whole afterwards Seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, each Cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection pollen sterility is thoroughly single 100 plants of listing marks of strain, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation pollen fertility, select pollen 30 plants of the individual plant that fertility is good, setting percentage is high, the neat mud of individual plant cut off sowing, paddy and rice straw are distinguished into listing mark, paddy goes out rough Carry out crude protein content measure respectively with rice straw afterwards, screening brown rice crude protein content is higher than 12%, and corresponding rice straw crude protein contains Strain 5 part of the amount higher than 7%, obtains BC2F4；
    L. strain plants 5 parts of BC2F4, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, selects agriculture Skill and yield traits is excellent, sterile phase pollen sterility is thorough and sterile conversion temperature and parent R similar in single-plant propagation seed, i.e., It is bred as the dual-purpose feed upland rice two-line sterile line " lyS " of paddy stalk;
    The high yield, brown rice gross protein value are more than feed rice varieties of the 12%, brown rice yield more than 80% for " gold early 47 " or " Hunan Early Xian 24 "；The glutathione transferasc kind is the " southeast 202 "；The upland rice variety of the high yield strong stress resistance is " Shanghai drought 7 " Or " facing drought 1 "；Described two be that maintainer is " Peiai 64S " or " good fortune eS1 ".
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1985576A (en) * 2006-08-15 2007-06-27 陈立云 Bilinear process of producing early rice hybrid seed
CN104137765A (en) * 2014-07-14 2014-11-12 福建农林大学 Breeding method of high-stress resistance elongated upper internode two-line genic male sterile line
CN104488702A (en) * 2015-01-15 2015-04-08 福建农林大学 Breeding method of dual-purpose sterile lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice
CN104839010A (en) * 2015-05-21 2015-08-19 福建金山都发展有限公司 Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1985576A (en) * 2006-08-15 2007-06-27 陈立云 Bilinear process of producing early rice hybrid seed
CN104137765A (en) * 2014-07-14 2014-11-12 福建农林大学 Breeding method of high-stress resistance elongated upper internode two-line genic male sterile line
CN104488702A (en) * 2015-01-15 2015-04-08 福建农林大学 Breeding method of dual-purpose sterile lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice
CN104839010A (en) * 2015-05-21 2015-08-19 福建金山都发展有限公司 Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line

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