CN105746341B - A kind of selection of ensilage upland rice two-line sterile line - Google Patents
A kind of selection of ensilage upland rice two-line sterile line Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention discloses a kind of selection of ensilage upland rice two-line sterile line, belong to Special Rices breeding technical field.The present invention makees basic parent from high yield, the feed rice varieties of high gross protein value, general parent is made with the high glutathione transferasc kind of the crisp rice varieties of stalk, protein contents of straw, High-yield Dry Land Rice kind, two-line sterile line, screened by the hybridization combo and objective trait of science, seed selection can make dry farming cultivation, the feed upland rice two-line sterile line as ensilage of high yield high nutrition, the restorer of the sterile line and same type assembles, and can be bred as the paddy of high yield high nutrition and can do forage rice, rice straw that the two-line hybrid rice upland rice variety of ensilage can be done.The inventive method is scientific and efficient, easily operated, and the seed selection to hybridization ensilage upland rice variety is significant.
Description
Technical field
Present invention relates particularly to a kind of selection of ensilage upland rice two-line sterile line, belong to Special Rices breeding technique
Field.
Background technology
Forage rice refers to breeding time is suitable, on the basis of resistance, using Optimum Cultivation, can realize (667 squares of high yield
More than 550 kilograms of yield of rice), high containing protein, high brown rice yield (being not less than 80%) and be adapted to feeding rice varieties." high egg
White forage rice " requires that brown rice gross protein value is more than or equal to 12%, the Breeding and popularization of " Forage Rice with High Protein Content ", not only may be used
Alleviate Indica rice because of poor taste and difficulty the problem of selling, can also realize that southern area substitutes setting for field corn with forage rice brown rice
Think.The live pig disease resistance raised with forage rice increases and meat taste is good, and brown rice raising is held substantially with corn feeding cost
It is flat, but development potentiality is huge.And all extract the aerial part of rice, grain, stalk, leaf mix as feed,
Custom is referred to as mixed fodder rice, and mixed fodder rice is compared with cutting green grass or young crops, hay, grain, rich in grain and fiber, nutritive value and thing
It is all more good to manage characteristic, is generally gathered in dough stage or full ripe stage, harvest is also more convenient, is the optimal side for making feed with rice
Case.The all more general straw of the gross protein value of Dual-purpose rice straw, protein digestibility, dry digestibility is high, paddy stalk
" physics duricrust " less, Relatively centralized that lignin and hemicellulose are combined closely by fat key in dual-purpose straw, so as to rice
" nutrition closing " degree of nutriment is smaller in grass, thus pepsin, cellulase etc. and glutathione transferasc grass meal connect
Contacting surface is big, and animal can therefrom obtain more nutriment.Thus, it can be made to cultivate mixed fodder rice using glutathione transferasc
Obtain its straw and more meet feed request.2400 tons of China's water resources ownership per capita, it is the 1/ of world's water resources ownership per capita
4, the 109th, the world is occupied, every year because economic loss caused by water shortage exceedes 200,000,000,000 yuan.And China's agricultural water accounts for total use at present
The 80% of water, wherein rice water consumption account for the 70% of agricultural water.It means that the production water consumption of only rice just accounts for the whole nation always
The 56% of water consumption, therefore with the existing water resource in China, it is difficult to support the further development of traditional rice.Upland rice variety need not
Sufficient moisture, water-saving amount can be ensured the another selection of grain security more than 50% as country, be developed using nonirrigated farmland high
Upland rice is produced, the new selection for maintaining national food security should be turned into.
The content of cellulose of paddy rice fragile straw mutant substantially reduces, and has good application prospect in animal feed preparation,
Because plant content of cellulose is lower, animal is higher to its digestibility, and in application test of the word with rice, research finds it
To improving the back fat fat hardness of pig, improving meat quality, the lean meat percentage raising effect especially to fat meat type is obvious, and these are excellent
Property not only contributes to improve the lean meat percentage of meat type pig, is also beneficial to the shelf life for extending meat.Ensilage is will be aqueous
Rate be 65%-75% green forage after chopping, under conditions of airtight anoxia, pass through the fermentation of anaerobism lactic acid bacteria, suppression
Make the breeding of various miscellaneous bacterias, and a kind of obtained roughage.Ensilage smell acid perfume, soft and succulency, palatability is good, nutrition is rich
It is rich, be domestic animal excellent food source beneficial to long-term preserve.Crisp stalk rice is highly suitable as because straw is easy to shred and digested
The raw material of ensilage.
Because the research work of forage rice is slower, breeding research is carried out not enough, and theoretical direction is lacked in seed selection, is again choosing
Educate work and add difficulty.The inventive method is more than the feed that 12%, brown rice yield is more than 80% with high yield, brown rice gross protein value
Rice varieties make basic parent, the glutathione transferasc kind for being adapted to make feed with straw, and the upland rice variety of high yield strong stress resistance makees one
As parent, screened by the hybridization combo and objective trait of science, seed selection can be with dry farming cultivation, the paddy stalk two of high yield high nutrition
With mixed fodder rice varieties.This method is scientific and efficient, and the seed selection to feed rice varieties is significant.
The content of the invention
It is an object of the invention to provide it is a kind of can with dry farming cultivation, combining go out high yield high nutrition ensilage it is miscellaneous
Hand over the selection of the two-line sterile line of rice varieties.
The present invention makees basic parent with the feed rice varieties of high yield, brown rice gross protein value more than 12%, is adapted to straw
Do the crisp stalk rice varieties of feed, glutathione transferasc kind, High-yield Dry Land Rice kind and make general parent, made not with excellent two system infertility
Educate gene and parent is provided, screened by the hybridization combo and objective trait of science, seed selection can make dry farming cultivation, high yield Gao Ying
The restorer of foster ensilage upland rice two-line sterile line, the sterile line and same type assembles, and can be bred as high yield high nutrition
Ensilage hybridizes upland rice variety.The selection of the present invention is scientific and efficient easy to operate, is provided for the seed selection of fodder hybrid rice
A kind of efficient new method, the seed selection to feed rice varieties have great importance.
Present disclosure：
The selection includes following methods and step：
（1）Juvenile stage：Select high yield, brown rice gross protein value more than 12% feed rice varieties " Hunan morning Xian 24 " or
" gold early 47 " makees basic parent（P）, crisp stalk rice varieties " in crisp B "（Peng Yingcai, crisp A passes through in dominant crisp stalk rice sterile line
Zhejiang Province's technical appraisement, Chinese rice, 2008 (5)：45）(P1), " southeast 201 " (P2), high yield resists glutathione transferasc kind
The inverse strong upland rice variety of property " Zhong Han 3 " or " Shanghai drought 3 " (P3) make general parent, two-line sterile line " Peiai 64S " or " good fortune
eS1”（P4）Make recurrent parent；
（2）Hybridize method for group matching：With feed rice varieties（P）Based on parent, to make genetic constitutions of the P in hybrid generation
Accounting is big, keeps its high yield high protein to integrate merit, using following hybridization method for group matching：
Hybridize for the first time：P×P1→F1-1； P×P2→F1-2；P×P3→F1-3；
Second of hybridization：P×F1-1→BF1-1； P×F1-2→BF1-2 ；P×F1-3→BF1-3 ；
Third time hybridizes：BF1-1 ×BF1-2→BF1-12；
4th hybridization：BF1-12×BF1-3→BF1-123；
5th hybridization：P ×BF3-123→B2F1-123；
6th hybridization：P4×BF4-123→BC1F1；
7th hybridization：P4×BC1F4→BC2F1 ；
（3）Plantation and filial generation system of selection：
A. kind plant parent P, P1, P2, P3, P4, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, P × P3 →
F1-3；Plantation hybridization F1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1 、P×F1-2And F1-3× P, it must return
Hand over F1For BF1-1 、BF1-2And BF1-3；
B. kind plants BF1-1 、BF1-2, florescence makees hybridization BF1-1 ×BF1-2→BF1-12；Plant BF1-12And BF1-3, bloom
Phase makees hybridization BF1-3 ×BF1-12→BF1-123；Plant BF1-123Obtain BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sow spray and poured a water, do not watered in whole seedling stage afterwards, 28 days seedling stages
Screening of drought resistance is carried out to rice shoot afterwards, removes tillering ability is poor, plant stalk is thin and delicate seedling, retain tillering ability by force, plant stalk and
The vigorous rice seedling of leaf growth, selects that rice straw is sturdy, blade keeps green not early ageing, stalk crisp and the individual plant of frangibility after ripe
200-220 strain individual plant sowings obtain BF3-123；
D. BF is planted in dry farming in a manner of strain3-123, each strain sows 90, sow to spray and poured a water, afterwards
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade
The green not early ageing of holding, stalk is crisp and 200 plants of neat mud of individual plant of the individual plant of frangibility cut off sowing, and paddy and rice straw are listed respectively
Mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly
Rice straw crude protein content be higher than 7% 30 parts of strain, obtain BF4-123；
E. strain mode dry farming plantation BF4-123, each strain sows 50, while plants parent P4, and florescence work hybridizes
P4×BF4-123Obtain BCF1, dry farming plantation BCF1, sowing obtains BCF2；The light seeding BCF on nonirrigated farmland2, sow spray and pour a water,
Whole seedling stage afterwards is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, and removal tillering ability is poor, plant stalk is thin and delicate
Seedling, retain tillering ability strong, plant stalk and the vigorous rice seedling of leaf growth, florescence selection stalk is crisp and frangibility, pollen
Thoroughly 80 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation pollen to infertility
Fertility, 10 sowings of individual plant that pollen fertility is good, setting percentage is high are selected to obtain BCF3；
F. BCF is planted in dry farming in a manner of strain3, each strain sows 100, sow to spray and poured a water, afterwards
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection stalk is crisp and easy to break
Thoroughly 100 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence to disconnected, pollen sterility
Pollen fertility is observed, selects 30 neat mud of strain of individual plant that pollen fertility is good, setting percentage is high to cut off sowing, paddy and rice straw is hung respectively
Card mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and right
The rice straw crude protein content answered is higher than 7% 2 parts of strain, obtains BCF4；
G. outdoor cultivation P4 and growth cabinet plantation BCF are distinguished4, florescence growth cabinet temperature setting is 23 DEG C,
Make hybridization P4 × BCF4, obtain BC1F1；BC is planted in dry farming1F1, obtain BC1F2；The light seeding BC on nonirrigated farmland1F2, sow spray and pour a water,
Do not watered in whole seedling stage afterwards, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removal tillering ability is poor, plant stalk is thin
Weak seedling, retain the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selection pollen sterility is thorough, stalk
Crisp and frangibility 80 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation flower
Powder fertility, 10 sowings of individual plant that pollen fertility is good, setting percentage is high are selected to obtain BCF3；
H. BCF is planted in dry farming in a manner of strain3, each strain sows 100, sow to spray and poured a water, afterwards
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selection pollen sterility thoroughly,
Stalk is crisp and 100 plants of listing marks of the individual plant of frangibility, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, and florescence is seen
Pollen fertility is examined, selects 30 neat mud of strain of individual plant that pollen fertility is good, setting percentage is high to cut off sowing, paddy and rice straw is listed respectively
Mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly
Rice straw crude protein content be higher than 7% 5 parts of strain, obtain BC1F4；
I. strain plants 5 parts of BC1F4, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, selected
Select agronomy and yield traits is excellent, sterile phase pollen sterility is thorough and sterile conversion temperature and parent P4 similar in single-plant propagation kind
Son is bred as ensilage upland rice two-line sterile line " ensiling S ".
Beneficial effects of the present invention：
Using the inventive method can select high yield, high nutrition ensilage upland rice two-line sterile line, the sterile line group
The crude protein content that the hybrid paddy rice matched somebody with somebody can make upland rice cultivation, yield height, paddy and straw is above common rice varieties, can be fully sharp
With dry land and mountain planting, kind of a rice water more than 60% is saved, makes full use of rice straw resource, every mu can increase by 300 yuan of income
More than.The selection science, easy, efficient, seed selection and national food security to ensilage upland rice variety have important
Meaning.
Embodiment
Embodiment 1：
（1）Juvenile stage：Basic parent is made in choosing " Hunan morning Xian 24 "（P）, " in crisp B " (P1), " southeast 201 " (P2),
" Zhong Han 3 " (P3) makees general parent, " Peiai 64S "（P4）Make recurrent parent；
（2）Plantation and filial generation selection：
A. kind plant parent P, P1, P2, P3, P4, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, P × P3 →
F1-3；Plantation hybridization F1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1 、P×F1-2And F1-3× P, it must return
Hand over F1For BF1-1 、BF1-2And BF1-3；
B. kind plants BF1-1 、BF1-2, florescence makees hybridization BF1-1 ×BF1-2→BF1-12；Plant BF1-12And BF1-3, bloom
Phase makees hybridization BF1-3 ×BF1-12→BF1-123；Plant BF1-123Obtain BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sow spray and poured a water, do not watered in whole seedling stage afterwards, 28 days seedling stages
Screening of drought resistance is carried out to rice shoot afterwards, removes tillering ability is poor, plant stalk is thin and delicate seedling, retain tillering ability by force, plant stalk and
The vigorous rice seedling of leaf growth, selects that rice straw is sturdy, blade keeps green not early ageing, stalk crisp and the individual plant of frangibility after ripe
200 plants of individual plant sowings obtain BF3-123；
D. BF is planted in dry farming in a manner of strain3-123, each strain sows 90, sow to spray and poured a water, afterwards
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade
The green not early ageing of holding, stalk is crisp and 200 plants of neat mud of individual plant of the individual plant of frangibility cut off sowing, and paddy and rice straw are listed respectively
Mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly
Rice straw crude protein content be higher than 7% 30 parts of strain, obtain BF4-123；
E. strain mode dry farming plantation BF4-123, each strain sows 50, while plants parent P4, and florescence work hybridizes
P4×BF4-123Obtain BCF1, dry farming plantation BCF1, sowing obtains BCF2；The light seeding BCF on nonirrigated farmland2, sow spray and pour a water,
Whole seedling stage afterwards is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, and removal tillering ability is poor, plant stalk is thin and delicate
Seedling, retain tillering ability strong, plant stalk and the vigorous rice seedling of leaf growth, florescence selection stalk is crisp and frangibility, pollen
Thoroughly 80 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation pollen to infertility
Fertility, 10 sowings of individual plant that pollen fertility is good, setting percentage is high are selected to obtain BCF3；
F. BCF is planted in dry farming in a manner of strain3, each strain sows 100, sow to spray and poured a water, afterwards
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection stalk is crisp and easy to break
Thoroughly 100 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence to disconnected, pollen sterility
Pollen fertility is observed, selects 30 neat mud of strain of individual plant that pollen fertility is good, setting percentage is high to cut off sowing, paddy and rice straw is hung respectively
Card mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and right
The rice straw crude protein content answered is higher than 7% 2 parts of strain, obtains BCF4；
G. outdoor cultivation P4 and growth cabinet plantation BCF are distinguished4, florescence growth cabinet temperature setting is 23 DEG C,
Make hybridization P4 × BCF4, obtain BC1F1；BC is planted in dry farming1F1, obtain BC1F2；The light seeding BC on nonirrigated farmland1F2, sow spray and pour a water,
Do not watered in whole seedling stage afterwards, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removal tillering ability is poor, plant stalk is thin
Weak seedling, retain the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selection pollen sterility is thorough, stalk
Crisp and frangibility 80 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation flower
Powder fertility, 10 sowings of individual plant that pollen fertility is good, setting percentage is high are selected to obtain BCF3；
H. BCF is planted in dry farming in a manner of strain3, each strain sows 100, sow to spray and poured a water, afterwards
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selection pollen sterility thoroughly,
Stalk is crisp and 100 plants of listing marks of the individual plant of frangibility, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, and florescence is seen
Pollen fertility is examined, selects 30 neat mud of strain of individual plant that pollen fertility is good, setting percentage is high to cut off sowing, paddy and rice straw is listed respectively
Mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly
Rice straw crude protein content be higher than 7% 5 parts of strain, obtain BC1F4；
I. strain plants 5 parts of BC1F4, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, selected
Select agronomy and yield traits is excellent, sterile phase pollen sterility is thorough and sterile conversion temperature and parent P4 similar in single-plant propagation kind
Son is bred as ensilage upland rice two-line sterile line " ensiling S-1 "；" ensiling S-1 " can assemble paddy stalk two with same type restorer
Hybridize upland rice variety with ensilage, the crude protein that the hybridization upland rice variety can make upland rice cultivation, yield height, paddy and straw contains
Amount is above common rice varieties, can make full use of dry land and mountain planting, saves kind of a rice water more than 60%, makes full use of rice straw
Resource, every mu can increase income more than 300 yuan.
Embodiment 2：
（1）Juvenile stage：" gold early 47 " makees basic parent（P）, " in crisp B " (P1), " southeast 201 " (P2), " Shanghai
Drought 3 " (P3) makees general parent, " good fortune eS1 "（P4）Make recurrent parent；
（2）Plantation and filial generation selection：
A. kind plant parent P, P1, P2, P3, P4, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, P × P3 →
F1-3；Plantation hybridization F1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1 、P×F1-2And F1-3× P, it must return
Hand over F1For BF1-1 、BF1-2And BF1-3；
B. kind plants BF1-1 、BF1-2, florescence makees hybridization BF1-1 ×BF1-2→BF1-12；Plant BF1-12And BF1-3, bloom
Phase makees hybridization BF1-3 ×BF1-12→BF1-123；Plant BF1-123Obtain BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sow spray and poured a water, do not watered in whole seedling stage afterwards, 28 days seedling stages
Screening of drought resistance is carried out to rice shoot afterwards, removes tillering ability is poor, plant stalk is thin and delicate seedling, retain tillering ability by force, plant stalk and
The vigorous rice seedling of leaf growth, selects that rice straw is sturdy, blade keeps green not early ageing, stalk crisp and the individual plant of frangibility after ripe
220 plants of individual plant sowings obtain BF3-123；
D. BF is planted in dry farming in a manner of strain3-123, each strain sows 90, sow to spray and poured a water, afterwards
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade
The green not early ageing of holding, stalk is crisp and 200 plants of neat mud of individual plant of the individual plant of frangibility cut off sowing, and paddy and rice straw are listed respectively
Mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly
Rice straw crude protein content be higher than 7% 30 parts of strain, obtain BF4-123；
E. strain mode dry farming plantation BF4-123, each strain sows 50, while plants parent P4, and florescence work hybridizes
P4×BF4-123Obtain BCF1, dry farming plantation BCF1, sowing obtains BCF2；The light seeding BCF on nonirrigated farmland2, sow spray and pour a water,
Whole seedling stage afterwards is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, and removal tillering ability is poor, plant stalk is thin and delicate
Seedling, retain tillering ability strong, plant stalk and the vigorous rice seedling of leaf growth, florescence selection stalk is crisp and frangibility, pollen
Thoroughly 80 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation pollen to infertility
Fertility, 10 sowings of individual plant that pollen fertility is good, setting percentage is high are selected to obtain BCF3；
F. BCF is planted in dry farming in a manner of strain3, each strain sows 100, sow to spray and poured a water, afterwards
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection stalk is crisp and easy to break
Thoroughly 100 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence to disconnected, pollen sterility
Pollen fertility is observed, selects 30 neat mud of strain of individual plant that pollen fertility is good, setting percentage is high to cut off sowing, paddy and rice straw is hung respectively
Card mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and right
The rice straw crude protein content answered is higher than 7% 2 parts of strain, obtains BCF4；
G. outdoor cultivation P4 and growth cabinet plantation BCF are distinguished4, florescence growth cabinet temperature setting is 23 DEG C,
Make hybridization P4 × BCF4, obtain BC1F1；BC is planted in dry farming1F1, obtain BC1F2；The light seeding BC on nonirrigated farmland1F2, sow spray and pour a water,
Do not watered in whole seedling stage afterwards, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removal tillering ability is poor, plant stalk is thin
Weak seedling, retain the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selection pollen sterility is thorough, stalk
Crisp and frangibility 80 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation flower
Powder fertility, 10 sowings of individual plant that pollen fertility is good, setting percentage is high are selected to obtain BCF3；
H. BCF is planted in dry farming in a manner of strain3, each strain sows 100, sow to spray and poured a water, afterwards
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, florescence selection pollen sterility thoroughly,
Stalk is crisp and 100 plants of listing marks of the individual plant of frangibility, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, and florescence is seen
Pollen fertility is examined, selects 30 neat mud of strain of individual plant that pollen fertility is good, setting percentage is high to cut off sowing, paddy and rice straw is listed respectively
Mark, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly
Rice straw crude protein content be higher than 7% 5 parts of strain, obtain BC1F4；
I. strain plants 5 parts of BC1F4, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, selected
Select agronomy and yield traits is excellent, sterile phase pollen sterility is thorough and sterile conversion temperature and parent P4 similar in single-plant propagation kind
Son is bred as ensilage upland rice two-line sterile line " ensiling S-2 "；" ensiling S-2 " can assemble paddy stalk two with same type restorer
Hybridize upland rice variety with mixed fodder, the crude protein that the hybridization upland rice variety can make upland rice cultivation, yield height, paddy and straw contains
Amount is above common rice varieties, can make full use of dry land and mountain planting, saves kind of a rice water more than 60%, makes full use of rice straw
Resource, every mu can increase income more than 300 yuan.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (1)
- A kind of 1. selection of ensilage upland rice two-line sterile line, it is characterised in that：The selection is included with lower section Method and step：（1）Juvenile stage：Select the feed rice varieties " Hunan morning Xian 24 " of high yield, brown rice gross protein value more than 12% or " gold is early 47 " make basic parent-P, crisp stalk rice varieties " in crisp B "-P1, glutathione transferasc the kind "-P2 of the southeast 201 ", high yield resistance Strong upland rice variety " Zhong Han 3 " or " Shanghai drought 3 "-P3 make general parent, two-line sterile line " Peiai 64S " or " good fortune eS1 "- P4 makees recurrent parent；（2）Hybridize method for group matching：The parent based on feed rice varieties P, to make genetic constitution accountings of the P in hybrid generation Greatly, its high yield high protein is kept to integrate merit, using following hybridization method for group matching：Hybridize for the first time：P×P1→F1-1； P×P2→F1-2；P×P3→F1-3；Second of hybridization：P×F1-1→BF1-1； P×F1-2→BF1-2 ；P×F1-3→BF1-3 ；Third time hybridizes：BF1-1 ×BF1-2→BF1-12；4th hybridization：BF1-12×BF1-3→BF1-123；5th hybridization：P ×BF3-123→B2F1-123；6th hybridization：P4×BF4-123→BC1F1；7th hybridization：P4×BC1F4→BC2F1 ；（3）Plantation and filial generation system of selection：A. kind plants parent P, P1, P2, P3, P4, makees hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, P × P3 → F1-3； Plantation hybridization F1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1、P×F1-2And F1-3× P, F must be returned1Generation BF1-1、BF1-2And BF1-3；B. kind plants BF1-1、BF1-2, florescence makees hybridization BF1-1×BF1-2→BF1-12；Plant BF1-12And BF1-3, florescence is made miscellaneous Hand over BF1-3 ×BF1-12→BF1-123；Plant BF1-123Obtain BF2-123；C. the light seeding BF on nonirrigated farmland2-123, sow spray and poured a water, do not watered in whole seedling stage afterwards, it is right after 28 days seedling stages Rice shoot carries out screening of drought resistance, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and blade Eugonic rice seedling, selects that rice straw is sturdy, blade keeps green not early ageing, stalk crisp and the individual plant 200- of frangibility after ripe 220 plants of individual plant sowings obtain BF3-123；D. BF is planted in dry farming in a manner of strain3-123, each strain sows 90, sow to spray and poured a water, whole afterwards Seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, each Cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and maturity period selection rice straw is sturdy, blade is kept Green not early ageing, stalk is crisp and 200 plants of neat mud of individual plant of the individual plant of frangibility cut off sowing, and paddy and rice straw are listed respectively and marked Note, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and corresponding Rice straw crude protein content is higher than 7% 30 parts of strain, obtains BF4-123；E. strain mode dry farming plantation BF4-123, each strain sows 50, while plants parent P4, florescence make hybridization P4 × BF4-123Obtain BCF1, dry farming plantation BCF1, sowing obtains BCF2；The light seeding BCF on nonirrigated farmland2, sowed spray and poured a water, afterwards Whole seedling stage do not water, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes that tillering ability is poor, plant stalk is thin and delicate Seedling, retains tillering ability strong, plant stalk and the vigorous rice seedling of leaf growth, florescence selection stalk is crisp and frangibility, pollen not Thoroughly 80 plants of listing marks of individual plant are educated, the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, and florescence observation pollen is educated Property, select 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BCF3；F. BCF is planted in dry farming in a manner of strain3, each strain sows 100, sow to spray and poured a water, whole afterwards Seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, each Cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selects stalk crisp and frangibility, flower Thoroughly 100 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation flower to powder infertility Powder fertility, select 30 neat mud of strain of individual plant that pollen fertility is good, setting percentage is high to cut off sowing, paddy and rice straw are listed respectively and marked Note, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and corresponding Rice straw crude protein content is higher than 7% 2 parts of strain, obtains BCF4；G. outdoor cultivation P4 and growth cabinet plantation BCF are distinguished4, florescence growth cabinet temperature setting is 23 DEG C, makees to hybridize P4×BCF4, obtain BC1F1；BC is planted in dry farming1F1, obtain BC1F2；The light seeding BC on nonirrigated farmland1F2, sowed spray and poured a water, afterwards Whole seedling stage do not water, screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes that tillering ability is poor, plant stalk is thin and delicate Seedling, retain tillering ability strong, plant stalk and the vigorous rice seedling of leaf growth, florescence selection pollen sterility is thorough, stalk is crisp and 80 plants of listing marks of individual plant of frangibility, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, and florescence observation pollen is educated Property, select 10 sowings of individual plant that pollen fertility is good, setting percentage is high to obtain BCF3；H. BCF is planted in dry farming in a manner of strain3, each strain sows 100, sow to spray and poured a water, whole afterwards Seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, each Cell retains 40 plants of the rice seedling that tillering ability is strong, plant stalk and leaf growth are vigorous, and florescence selection pollen sterility is thorough, stalk Crisp and frangibility 100 plants of listing marks of individual plant, the growth cabinet that shift-in temperature is 23 DEG C cut pocket regeneration, florescence observation flower Powder fertility, select 30 neat mud of strain of individual plant that pollen fertility is good, setting percentage is high to cut off sowing, paddy and rice straw are listed respectively and marked Note, paddy go out rough rear and rice straw and carry out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and corresponding Rice straw crude protein content is higher than 7% 5 parts of strain, obtains BC1F4；I. strain plants 5 parts of BC1F4, investigation screening is carried out to its economical character, pollen fertility, sterile conversion temperature, selects agriculture Skill and yield traits is excellent, sterile phase pollen sterility is thorough and sterile conversion temperature and parent P4 similar in single-plant propagation seed be It is bred as ensilage upland rice two-line sterile line " ensiling S ".
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