CN105746336B - A kind of selection of ensilage upland rice CMS line - Google Patents
A kind of selection of ensilage upland rice CMS line Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
The invention discloses a kind of selection of ensilage upland rice CMS line, belong to Special Rices breeding technical field.The present invention makees basic parent from the feed rice varieties of high yield, high gross protein value, it is that maintainer makees general parent with the crisp rice varieties of stalk, glutathione transferasc kind, High-yield Dry Land Rice kind, three, screened by the hybridization combo and objective trait of science, seed selection can make dry farming cultivation, high yield high nutrition can as ensilage feed upland rice CMS line, the restorer of the sterile line and same type is assembled, and can be bred as paddy can do the ensilage triple crossing upland rice variety that forage rice, rice straw can do the high yield high nutrition of ensilage.The inventive method is scientific and efficient, it is easy to operate, and the seed selection to hybridization ensilage upland rice variety is significant.
Description
Technical field
Present invention relates particularly to a kind of selection of ensilage upland rice CMS line, belong to Special Rices breeding technique
Field.
Background technology
Forage rice refers to breeding time is suitable, on the basis of resistance, using Optimum Cultivation, can realize (667 squares of high yield
More than 550 kilograms of yield of rice), high containing protein, high brown rice yield (being not less than 80%) and be adapted to feeding rice varieties." high egg
White forage rice " requires that brown rice gross protein value is more than or equal to 12%, and the Breeding and popularization of " Forage Rice with High Protein Content " not only may be used
Alleviate Indica rice the problem of difficulty is sold because of poor taste, can also realize that southern area substitutes setting for field corn with forage rice brown rice
Think.The live pig disease resistance raised with forage rice increases and meat taste is good, and brown rice is raised and held substantially with corn feeding cost
It is flat, but development potentiality is huge.And all extract the aerial part of paddy rice, grain, stalk, leaf mix as feed,
Custom is referred to as mixed fodder rice, and mixed fodder rice is compared with cutting green grass or young crops, hay, grain, rich in grain and fiber, nutritive value and thing
Manage characteristic all more good, generally gathered in dough stage or full ripe stage, harvest is also more convenient, is the optimal side for making feed with rice
Case.The all more general straw of the gross protein value of Dual-purpose rice straw, protein digestibility, dry digestibility is high, paddy stalk
" physics duricrust " less, Relatively centralized that lignin and hemicellulose are combined closely by fat key in dual-purpose straw, so as to rice
" nutrition closing " degree of nutriment is smaller in grass, thus pepsin, cellulase etc. and glutathione transferasc grass meal connect
Contacting surface is big, and animal can therefrom obtain more nutriment.Thus, mixed fodder rice is cultivated using glutathione transferasc to be made
Obtain its straw and more meet feed request.2400 tons of China's water resources ownership per capita, is the 1/ of world's water resources ownership per capita
4, the 109th, the world is occupied, every year because the economic loss that water shortage is caused exceedes 200,000,000,000 yuan.And China's agricultural water accounts for total use at present
The 80% of water, wherein paddy rice water consumption account for the 70% of agricultural water.It means that the production water consumption of only paddy rice just accounts for the whole nation always
The 56% of water consumption, therefore with the existing water resource of China, it is difficult to the further development of the traditional paddy rice of support.Upland rice variety need not
Sufficient moisture, water-saving amount can ensure the another selection of grain security more than 50% as country, be developed using nonirrigated farmland high
Upland rice is produced, the new selection for maintaining national food security should be turned into.
The content of cellulose of paddy rice fragile straw mutant is substantially reduced, and has good application prospect in animal feed preparation,
Because plant content of cellulose is lower, animal is higher to its digestibility, in the application test to Fodder Rice, and research finds it
To improving the back fat fat hardness of pig, improving meat quality, the especially lean meat percentage raising to fat meat type acts on obvious, and these are excellent
Property not only contributes to improve the lean meat percentage of meat type pig, also beneficial to the shelf life of extension meat.Ensilage is will be aqueous
Rate for 65%-75% green forage after chopping, under conditions of airtight anoxia, pass through the fermentation of anaerobism lactic acid bacteria, suppression
Make a kind of roughage obtained from the breeding of various miscellaneous bacterias.Ensilage smell acid perfume, soft and succulency, palatability are good, nutrition is rich
It is rich, be domestic animal excellent food source beneficial to long-term preservation.Crisp stalk paddy rice is highly suitable as because straw is easy to shred and digested
The raw material of ensilage.
Because the research work of forage rice is started late, breeding research development is insufficient, and theoretical direction is lacked in seed selection,
Again difficulty is added for seed selection work.The inventive method is more than 12%, brown rice yield with high yield, brown rice gross protein value and is more than 80%
Feed rice varieties make basic parent, the glutathione transferasc kind for being adapted to make feed with straw, the upland rice product of high yield strong stress resistance
Kind make general parent, screened by the hybridization combo and objective trait of science, seed selection can with dry farming cultivation, high yield high nutrition
The dual-purpose mixed fodder rice varieties of paddy stalk.This method is scientific and efficient, and the seed selection to feed rice varieties is significant.
The content of the invention
It is an object of the invention to provide it is a kind of can be with dry farming cultivation, the ensilage that combining goes out high yield high nutrition is miscellaneous
Hand over the selection of the CMS line of rice varieties.
The present invention makees basic parent with feed rice varieties, dual-purpose with crisp stalk rice varieties, the paddy stalk that straw is adapted to do feed
Rice varieties, High-yield Dry Land Rice kind, three are that maintainer makees general parent, are screened by the hybridization combo and objective trait of science, choosing
Dry farming cultivation can be made by educating, the ensilage upland rice CMS line of high yield high nutrition, the sterile line and same type it is extensive
Multiple system assembles, and can be bred as the ensilage hybridization upland rice variety of high yield high nutrition.The scientific and efficient easy behaviour of selection of the present invention
Make, provide a kind of efficient new method for the seed selection of fodder hybrid rice, there is important guiding meaning to the seed selection of feed rice varieties
Justice.
For achieving the above object, the present invention is adopted the following technical scheme that：
（1）Juvenile stage：Select high yield, brown rice gross protein value be more than 12% feed rice varieties " Hunan morning Xian 24 " or
" gold early 47 " makees basic parent（P）, crisp stalk rice varieties " in crisp B " (P1), glutathione transferasc kind " southeast 201 " or " southeast
202 " (P2), the upland rice variety " Zhong Han 3 " of high yield strong stress resistance or " Shanghai drought seven " (P3) make general parent, and three be to keep
System " flower 1B " or " golden 23B "（P4）Make recurrent parent；
The present invention hybridization combo thinking be：With feed rice varieties（P）Based on parent, to make P in hybrid generation
Genetic constitution accounting is big, keeps its high yield high protein to integrate merit, using following hybridization method for group matching：
Hybridize for the first time：P×P1→F1-1；P×P2→F1-2；P×P3→F1-3；
Second of hybridization：P×F1-1→BF1-1；P×F1-2→BF1-2；P×F1-3→BF1-3；
Third time hybridizes：BF1-1×BF1-2→BF1-12；
4th hybridization：BF1-12×BF1-3→BF1-123；
5th hybridization：P4×BF4-123→BC1F1；
6th hybridization：P4×BC1F3→BC2F1；
7th hybridization：Sterile line A × maintainer QCB is simultaneously returned 4 generations → QCA；
（2）Plantation and filial generation system of selection：
A. plant and plant parent P, P1, P2, P3, P4, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, P × P3 →
F1-3；Plantation hybridization F1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1、P×F1-2With P × F1-3, must be returned
F1For BF1-1、BF1-2And BF1-3；
B. plant and plant BF1-1、BF1-2, florescence makees hybridization BF1-1×BF1-2→BF1-12；Plant BF1-12And BF1-3, florescence
Make hybridization BF1-3×BF1-12→BF1-123；Plant BF1-123Sowing obtains BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sowed spray and poured a water, the whole seedling stage after does not water, 28 days seedling stages
Screening of drought resistance is carried out to rice shoot afterwards, tillering ability is poor, plant stalk is thin and delicate seedling is removed, retain tillering ability by force, plant stalk and
The vigorous rice seedling of leaf growth, it is ripe after selection rice straw is sturdy, blade keeps green not early ageing, stalk crisp and frangibility individual plant
180-200 plants of individual plant sowings obtain BF3-123；
D. BF is planted with the dry farming of strain mode3-123, each strain sows 90, sow to spray and poured a water, after
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains strong tillering ability, plant stalk and 40 plants vigorous of rice seedling of leaf growth, and maturity period selection rice straw is sturdy, blade
Keep green not early ageing, stalk crisp and frangibility 180 plants of neat mud of individual plant of individual plant cut off sowing, paddy and rice straw are listed respectively
Mark, paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly
Rice straw crude protein content be higher than 7% 20 parts of strain, obtain BF4-123；
E. BF is planted in the dry farming of strain mode4-123, each strain sows 50, while planting parent P4, florescence selection is excellent
Different individual plant makees hybridization P4 × BF4-123Obtain BC1F1, dry farming plantation BC1F1, sowing obtains BC1F2；The light seeding BC on nonirrigated farmland1F2, sowed
A water is poured in spray, and the whole seedling stage after does not water, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes tillering ability
The thin and delicate seedling of difference, plant stalk, retains strong tillering ability, plant stalk and the vigorous rice seedling of leaf growth, maturity period selection rice straw
Sturdy, blade keeps green not early ageing, stalk crisp and frangibility 160 plants of neat mud of individual plant of individual plant cut off sowing, by paddy and rice
Stalk distinguishes listing mark, and paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than
12%, and corresponding rice straw crude protein content is higher than 7% 10 parts of strain, obtains BC1F3；
F. BC is planted with the dry farming of strain mode1F3, each strain sows 50, sow to spray and poured a water, after
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains strong tillering ability, plant stalk and 20 plants vigorous of rice seedling of leaf growth, and florescence selection stalk is crisp and easy to break
Disconnected elite seedling makees hybridization P4 × BC1F3Obtain BC2F1, plant BC2F1, sowing obtains BC2F2；The light seeding BC on nonirrigated farmland2F2, sowing
A water is poured in complete spray, and the whole seedling stage after does not water, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes tillering ability
The thin and delicate seedling of difference, plant stalk, retains strong tillering ability, plant stalk and the vigorous rice seedling of leaf growth, maturity period selection rice straw
Sturdy, blade keeps green not early ageing, stalk crisp and frangibility 140 plants of neat mud of individual plant of individual plant cut off sowing, by paddy and rice
Stalk distinguishes listing mark, and paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than
12%, and corresponding rice straw crude protein content is higher than 7% 10 parts of strain, obtains BC2F3；
G. BC is planted with the dry farming of strain mode2F3, each strain sows 100, sow to spray and poured a water, after
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains strong tillering ability, plant stalk and 20 plants vigorous of rice seedling of leaf growth, the high list of maturity period selection setting percentage
60 plants of neat mud of strain cut off sowing, and paddy and rice straw are distinguished into listing mark, and paddy goes out rough rear and rice straw to carry out crude protein and contain respectively
It is fixed to measure, and screening brown rice crude protein content is higher than 12%, and corresponding rice straw crude protein content is higher than 7% 1-2 parts of strain, that is, educates
It is maintainer QCB into ensilage upland rice three；
H. plant and plant QCB former sterile line A corresponding with its, make hybridization A × QCB and be returned for 4 generations, that is, be bred as ensilage upland rice
CMS line QCA.
Beneficial effects of the present invention：
Using the inventive method can select high yield, high nutrition ensilage upland rice CMS line, the sterile line group
The crude protein content that the hybrid paddy rice matched somebody with somebody can make upland rice cultivation, high yield, paddy and straw is above common rice varieties, can be fully sharp
With dry land and mountain planting, kind of a rice water more than 60% is saved, rice straw resource is made full use of, every mu can increase by 400 yuan of income
More than；The selection science, easy, efficient, seed selection and national food security to ensilage upland rice variety have important
Meaning.
Embodiment
The present invention further illustrates the present invention with the following example, but protection scope of the present invention is not limited to following reality
Apply example.
Embodiment 1
（1）Juvenile stage：" Hunan morning Xian 24 " is selected to make basic parent（P）, " in crisp B " (P1)（Peng Yingcai, dominant crisp stalk
Crisp A passes through Zhejiang Province's technical appraisement, Chinese rice, 2008 (5) in rice sterile line：45）, " southeast 201 " (P2), " middle non-irrigated 3
Number " (P3), " flower 1B " make general parent；
（2）Plantation and filial generation selection：
A. plant and plant parent P, P1, P2, P3, P4, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, P × P3 →
F1-3；Plantation hybridization F1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1、P×F1-2And F1-3× P, must be returned
F1For BF1-1、BF1-2And BF1-3；
B. plant and plant BF1-1、BF1-2, florescence makees hybridization BF1-1×BF1-2→BF1-12；Plant BF1-12And BF1-3, florescence
Make hybridization BF1-3×BF1-12→BF1-123；Plant BF1-123Sowing obtains BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sowed spray and poured a water, the whole seedling stage after does not water, 28 days seedling stages
Screening of drought resistance is carried out to rice shoot afterwards, tillering ability is poor, plant stalk is thin and delicate seedling is removed, retain tillering ability by force, plant stalk and
The vigorous rice seedling of leaf growth, it is ripe after selection rice straw is sturdy, blade keeps green not early ageing, stalk crisp and frangibility individual plant
180 plants of individual plant sowings obtain BF3-123；
D. BF is planted with the dry farming of strain mode3-123, each strain sows 90, sow to spray and poured a water, after
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains strong tillering ability, plant stalk and 40 plants vigorous of rice seedling of leaf growth, and maturity period selection rice straw is sturdy, blade
Keep green not early ageing, stalk crisp and frangibility 180 plants of neat mud of individual plant of individual plant cut off sowing, paddy and rice straw are listed respectively
Mark, paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly
Rice straw crude protein content be higher than 7% 20 parts of strain, obtain BF4-123；
E. BF is planted in the dry farming of strain mode4-123, each strain sows 50, while planting parent P4, florescence selection is excellent
Different individual plant makees hybridization P4 × BF4-123Obtain BC1F1, dry farming plantation BC1F1, sowing obtains BC1F2；The light seeding BC on nonirrigated farmland1F2, sowed
A water is poured in spray, and the whole seedling stage after does not water, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes tillering ability
The thin and delicate seedling of difference, plant stalk, retains strong tillering ability, plant stalk and the vigorous rice seedling of leaf growth, maturity period selection rice straw
Sturdy, blade keeps green not early ageing, stalk crisp and frangibility 160 plants of neat mud of individual plant of individual plant cut off sowing, by paddy and rice
Stalk distinguishes listing mark, and paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than
12%, and corresponding rice straw crude protein content is higher than 7% 10 parts of strain, obtains BC1F3；
F. BC is planted with the dry farming of strain mode1F3, each strain sows 50, sow to spray and poured a water, after
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains strong tillering ability, plant stalk and 20 plants vigorous of rice seedling of leaf growth, and florescence selection stalk is crisp and easy to break
Disconnected elite seedling makees hybridization P4 × BC1F3Obtain BC2F1, plant BC2F1, sowing obtains BC2F2；The light seeding BC on nonirrigated farmland2F2, sowing
A water is poured in complete spray, and the whole seedling stage after does not water, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes tillering ability
The thin and delicate seedling of difference, plant stalk, retains strong tillering ability, plant stalk and the vigorous rice seedling of leaf growth, maturity period selection rice straw
Sturdy, blade keeps green not early ageing, stalk crisp and frangibility 140 plants of neat mud of individual plant of individual plant cut off sowing, by paddy and rice
Stalk distinguishes listing mark, and paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than
12%, and corresponding rice straw crude protein content is higher than 7% 10 parts of strain, obtains BC2F3；
G. BC is planted with the dry farming of strain mode2F3, each strain sows 100, sow to spray and poured a water, after
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains strong tillering ability, plant stalk and 20 plants vigorous of rice seedling of leaf growth, the high list of maturity period selection setting percentage
60 plants of neat mud of strain cut off sowing, and paddy and rice straw are distinguished into listing mark, and paddy goes out rough rear and rice straw to carry out crude protein and contain respectively
It is fixed to measure, and screening brown rice crude protein content is higher than 12%, and corresponding rice straw crude protein content is higher than 7% 1-2 parts of strain, that is, educates
It is maintainer QCB-1 into ensilage upland rice three；
H. plant and plant QCB-1 and flower 1A, make hybridization flower 1A × QCB and be returned for 4 generations, that is, it is infertility to be bred as ensilage upland rice three
It is QCA-1；
QCA-1 can assemble the dual-purpose ensilage hybridization upland rice variety of paddy stalk, the hybridization upland rice variety with same type restorer
The crude protein content that upland rice cultivation, high yield, paddy and straw can be made is above common rice varieties, can make full use of dry land and mountain
Ground is planted, and is saved kind of a rice water more than 60%, is made full use of rice straw resource, every mu can increase income more than 400 yuan.
Embodiment 2
（1）Juvenile stage：" gold early 47 " makees basic parent（P）, " in crisp B " (P1), " southeast 202 " (P2), " and Shanghai drought
No. seven " (P3), " golden 23B "（P4）Make general parent；
（2）Plantation and filial generation selection：
A. plant and plant parent P, P1, P2, P3, P4, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, P × P3 →
F1-3；Plantation hybridization F1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1、P×F1-2And F1-3× P, must be returned
F1For BF1-1、BF1-2And BF1-3；
B. plant and plant BF1-1、BF1-2, florescence makees hybridization BF1-1×BF1-2→BF1-12；Plant BF1-12And BF1-3, florescence
Make hybridization BF1-3 ×BF1-12→BF1-123；Plant BF1-123Sowing obtains BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sowed spray and poured a water, the whole seedling stage after does not water, 28 days seedling stages
Screening of drought resistance is carried out to rice shoot afterwards, tillering ability is poor, plant stalk is thin and delicate seedling is removed, retain tillering ability by force, plant stalk and
The vigorous rice seedling of leaf growth, it is ripe after selection rice straw is sturdy, blade keeps green not early ageing, stalk crisp and frangibility individual plant
200 plants of individual plant sowings obtain BF3-123；
D. BF is planted with the dry farming of strain mode3-123, each strain sows 90, sow to spray and poured a water, after
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains strong tillering ability, plant stalk and 40 plants vigorous of rice seedling of leaf growth, and maturity period selection rice straw is sturdy, blade
Keep green not early ageing, stalk crisp and frangibility 180 plants of neat mud of individual plant of individual plant cut off sowing, paddy and rice straw are listed respectively
Mark, paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than 12%, and correspondingly
Rice straw crude protein content be higher than 7% 20 parts of strain, obtain BF4-123；
E. BF is planted in the dry farming of strain mode4-123, each strain sows 50, while planting parent P4, florescence selection is excellent
Different individual plant makees hybridization P4 × BF4-123Obtain BC1F1, dry farming plantation BC1F1, sowing obtains BC1F2；The light seeding BC on nonirrigated farmland1F2, sowed
A water is poured in spray, and the whole seedling stage after does not water, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes tillering ability
The thin and delicate seedling of difference, plant stalk, retains strong tillering ability, plant stalk and the vigorous rice seedling of leaf growth, maturity period selection rice straw
Sturdy, blade keeps green not early ageing, stalk crisp and frangibility 160 plants of neat mud of individual plant of individual plant cut off sowing, by paddy and rice
Stalk distinguishes listing mark, and paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than
12%, and corresponding rice straw crude protein content is higher than 7% 10 parts of strain, obtains BC1F3；
F. BC is planted with the dry farming of strain mode1F3, each strain sows 50, sow to spray and poured a water, after
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains strong tillering ability, plant stalk and 20 plants vigorous of rice seedling of leaf growth, and florescence selection stalk is crisp and easy to break
Disconnected elite seedling makees hybridization P4 × BC1F3Obtain BC2F1, plant BC2F1, sowing obtains BC2F2；The light seeding BC on nonirrigated farmland2F2, sowing
A water is poured in complete spray, and the whole seedling stage after does not water, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes tillering ability
The thin and delicate seedling of difference, plant stalk, retains strong tillering ability, plant stalk and the vigorous rice seedling of leaf growth, maturity period selection rice straw
Sturdy, blade keeps green not early ageing, stalk crisp and frangibility 140 plants of neat mud of individual plant of individual plant cut off sowing, by paddy and rice
Stalk distinguishes listing mark, and paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than
12%, and corresponding rice straw crude protein content is higher than 7% 10 parts of strain, obtains BC2F3；
G. BC is planted with the dry farming of strain mode2F3, each strain sows 100, sow to spray and poured a water, after
Whole seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate,
Each cell retains strong tillering ability, plant stalk and 20 plants vigorous of rice seedling of leaf growth, the high list of maturity period selection setting percentage
60 plants of neat mud of strain cut off sowing, and paddy and rice straw are distinguished into listing mark, and paddy goes out rough rear and rice straw to carry out crude protein and contain respectively
It is fixed to measure, and screening brown rice crude protein content is higher than 12%, and corresponding rice straw crude protein content is higher than 7% 1-2 parts of strain, that is, educates
It is maintainer QCB-2 into ensilage upland rice three；
H. plant and plant QCB-2 and gold 23A, make hybridization gold 23A × QCB-2 and be returned for 4 generations, that is, be bred as ensilage upland rice three
It is sterile line QCA-2；
QCA-2 can assemble the dual-purpose mixed fodder hybridization upland rice variety of paddy stalk, the hybridization upland rice variety with same type restorer
The crude protein content that upland rice cultivation, high yield, paddy and straw can be made is above common rice varieties, can make full use of dry land and mountain
Ground is planted, and is saved kind of a rice water more than 60%, is made full use of rice straw resource, every mu can increase income more than 400 yuan.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (1)
1. a kind of selection of ensilage upland rice CMS line, it is characterised in that：The selection includes following step
Suddenly：
（1）Juvenile stage：The feed rice varieties " Hunan morning Xian 24 " or " golden early for selecting high yield, brown rice gross protein value to be more than 12%
47 " make basic parent P, crisp stalk rice varieties " in crisp B " P1, glutathione transferasc kind " southeast 201 " or " P2 of the southeast 202 ", it is high
The upland rice variety " Zhong Han 3 " or " Shanghai drought seven " P3 for producing strong stress resistance make general parent, and three be maintainer " flower 1B " or " gold
23B " P4 make recurrent parent；
（2）Plantation and the selection of filial generation：
A. plant and plant parent P, P1, P2, P3, P4, make hybridization P × P1 → F in florescence1-1, P × P2 → F1-2, P × P3 → F1-3；
Plantation hybridization F1For F1-1、F1-2、F1-3With parent P, make hybridization P × F in florescence1-1 、P×F1-2With P × F1-3, F must be returned1
For BF1-1、BF1-2And BF1-3；
B. plant and plant BF1-1、BF1-2, florescence makees hybridization BF1-1×BF1-2→BF1-12；Plant BF1-12And BF1-3, florescence is made miscellaneous
Hand over BF1-3×BF1-12→BF1-123；Plant BF1-123Sowing obtains BF2-123；
C. the light seeding BF on nonirrigated farmland2-123, sowed spray and poured a water, the whole seedling stage after does not water, right after 28 days seedling stages
Rice shoot carries out screening of drought resistance, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, retains strong tillering ability, plant stalk and blade
Eugonic rice seedling, it is ripe after selection rice straw is sturdy, blade keeps green not early ageing, stalk crisp and frangibility individual plant sowing
Obtain BF3-123；
D. BF is planted with the dry farming of strain mode3-123, sowed spray and poured a water, the whole seedling stage after does not water, seedling stage 28
Screening of drought resistance is carried out to rice shoot after it, tillering ability is poor, plant stalk is thin and delicate seedling is removed, each cell retain tillering ability by force,
Plant stalk and the vigorous rice seedling of leaf growth, maturity period selection rice straw is sturdy, blade keeps green not early ageing, stalk crisp and easy
The neat mud of individual plant fractureed cuts off sowing, and paddy and rice straw are distinguished into listing mark, and paddy goes out rough rear and rice straw and carries out thick egg respectively
White assay, screening brown rice crude protein content is higher than 12%, and corresponding rice straw crude protein content is higher than 7% strain, obtains
BF4-123；
E. BF is planted in the dry farming of strain mode4-123, while planting parent P4, florescence selection elite seedling makees hybridization P4 × BF4-123
Obtain BC1F1, dry farming plantation BC1F1, sowing obtains BC1F2；The light seeding BC on nonirrigated farmland1F2, sow spray and pour a water, it is whole after
Individual seedling stage is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, is protected
Strong tillering ability, plant stalk and the vigorous rice seedling of leaf growth are stayed, maturity period selection rice straw is sturdy, blade keeps green late
Decline, stalk is crisp and the neat mud of individual plant of frangibility cuts off sowing, paddy and rice straw distinguished into listing mark, paddy go out it is rough after and rice straw
Crude protein content measure is carried out respectively, and screening brown rice crude protein content is higher than 12%, and corresponding rice straw crude protein content is higher than 7%
Strain, obtain BC1F3；
F. BC is planted with the dry farming of strain mode1F3, sowed spray and poured a water, the whole seedling stage after does not water, seedling stage 28
Screening of drought resistance is carried out to rice shoot after it, tillering ability is poor, plant stalk is thin and delicate seedling is removed, each cell retain tillering ability by force,
Plant stalk and the vigorous rice seedling of leaf growth, florescence selects stalk crisp and frangibility elite seedling makees hybridization P4 × BC1F3
Obtain BC2F1, plant BC2F1, sowing obtains BC2F2；The light seeding BC on nonirrigated farmland2F2, sow spray and pour a water, the whole seedling after
Phase is not watered, and screening of drought resistance is carried out to rice shoot after 28 days seedling stages, removes the seedling that tillering ability is poor, plant stalk is thin and delicate, is retained and is divided
Tiller power is strong, plant stalk and the vigorous rice seedling of leaf growth, and maturity period selection rice straw is sturdy, blade keeps green not early ageing, stem
Stalk is crisp and the neat mud of individual plant of frangibility cuts off sowing, and paddy and rice straw distinguished into listing mark, paddy go out it is rough after and rice straw distinguish
Crude protein content measure is carried out, screening brown rice crude protein content is higher than 12%, and corresponding rice straw crude protein content is higher than 7% product
System, obtains BC2F3；
G. BC is planted with the dry farming of strain mode2F3, sowed spray and poured a water, the whole seedling stage after does not water, seedling stage 28
Screening of drought resistance is carried out to rice shoot after it, tillering ability is poor, plant stalk is thin and delicate seedling is removed, each cell retain tillering ability by force,
Plant stalk and the vigorous rice seedling of leaf growth, the high neat mud of individual plant of maturity period selection setting percentage cut off sowing, by paddy and rice
Stalk distinguishes listing mark, and paddy goes out rough rear and rice straw and carries out crude protein content measure respectively, and screening brown rice crude protein content is higher than
12%, and corresponding rice straw crude protein content is higher than 7% strain, that is, it is maintainer QCB to be bred as ensilage upland rice three；
H. plant and plant QCB former sterile line A corresponding with its, make hybridization A × QCB and be returned for 4 generations, that is, being bred as ensilage upland rice three is
Sterile line QCA.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102630556A (en) * | 2012-05-12 | 2012-08-15 | 福建农林大学 | Breeding method of multi-resistance glutinous rice restoring line |
| CN103125368A (en) * | 2013-03-13 | 2013-06-05 | 福建农林大学 | Breeding method of insect-resistant big-stigma three-line sterile line |
| CN103229713A (en) * | 2013-05-06 | 2013-08-07 | 福建农林大学 | Selective breeding method for high-nutrition rice varieties |
| CN103931490A (en) * | 2014-04-04 | 2014-07-23 | 福建农林大学 | Breeding method of resistance-variable rice variety |
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| CN102630556A (en) * | 2012-05-12 | 2012-08-15 | 福建农林大学 | Breeding method of multi-resistance glutinous rice restoring line |
| CN103125368A (en) * | 2013-03-13 | 2013-06-05 | 福建农林大学 | Breeding method of insect-resistant big-stigma three-line sterile line |
| CN103229713A (en) * | 2013-05-06 | 2013-08-07 | 福建农林大学 | Selective breeding method for high-nutrition rice varieties |
| CN103931490A (en) * | 2014-04-04 | 2014-07-23 | 福建农林大学 | Breeding method of resistance-variable rice variety |
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